CN203551507U - Simple temperature control PCR-SSCP gel electrophoresis device - Google Patents

Simple temperature control PCR-SSCP gel electrophoresis device Download PDF

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Publication number
CN203551507U
CN203551507U CN201320756394.4U CN201320756394U CN203551507U CN 203551507 U CN203551507 U CN 203551507U CN 201320756394 U CN201320756394 U CN 201320756394U CN 203551507 U CN203551507 U CN 203551507U
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China
Prior art keywords
gel electrophoresis
sscp
temperature control
case body
control pcr
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Expired - Fee Related
Application number
CN201320756394.4U
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Chinese (zh)
Inventor
刘彬
罗卫星
孟源
宋桃伟
孙岩岩
康建兵
钱成
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Guizhou University
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Guizhou University
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Priority to CN201320756394.4U priority Critical patent/CN203551507U/en
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Publication of CN203551507U publication Critical patent/CN203551507U/en
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Abstract

The utility model discloses a simple temperature control PCR-SSCP gel electrophoresis device which comprises a gel electrophoresis apparatus (1), a gel electrophoresis tank (8) and a gel electrophoresis box. The gel electrophoresis tank (8) is located in a gel electrophoresis box body (3). A gap (7) is formed between the gel electrophoresis tank (8) and the inner wall of the gel electrophoresis box body. A positive electrode port and a negative electrode port (11) in the gel electrophoresis tank (8) are respectively electrically connected with a positive electrode port and a negative electrode port (10) on the gel electrophoresis apparatus (1) through a power line (9) and a power line outlet (6) at the lower end of the gel electrophoresis box body (3). An observing window (4) is arranged in the front side of the gel electrophoresis box body (3), and a thermometer (5) is disposed on the inner side of the observing window. The simple temperature control PCR-SSCP gel electrophoresis device has the advantages of solving the problems that the operation based on existing experiment methods is not convenient, price is too high, and the low-temperature electrophoresis condition cannot be met, and also has the advantages of being simple in structure, convenient, practical, low in price, and enabling temperature to be conveniently adjusted.

Description

Simple and easy temperature control PCR-SSCP gel electrophoresis apparatus
Technical field
The utility model relates to a kind of simple and easy PCR-SSCP gel electrophoresis apparatus of experiment gel electrophoresis apparatus, especially controllable temperature.
Background technology
Existing laboratory gel electrophoresis apparatus often can only be satisfied with the gel electrophoresis experiment under normal temperature state, and at present large quantity research shows, effect is carried out in PCR-SSCP gel electrophoresis experiment under cryogenic conditions can be better.Most laboratory adopts the interior electrophoresis of 4 ℃ of refrigerators to meet the condition of low temperature now, and the method exists many deficiencies, and for example refrigerator volume is excessive, not easy to operate; Refrigerator price is too high, fragile; Gel electrophoresis groove need be placed in operation in refrigerator, adjusts inconvenience, cannot monitor etc.Therefore, for better realizing PCR-SSCP gel electrophoresis experiment, carry out at low temperatures, be necessary to study the simple and easy PCR-SSCP gel electrophoresis apparatus of controllable temperature.
Summary of the invention
The technical problems to be solved in the utility model: a kind of simple and easy temperature control PCR-SSCP gel electrophoresis apparatus is provided, simple in structure, convenient and practical, cheap, can make PCR-SSCP gel electrophoresis experiment carry out at low temperatures, to overcome the deficiencies in the prior art.
The technical solution of the utility model:
Simple and easy temperature control PCR-SSCP gel electrophoresis apparatus, comprise gel-electrophoretic apparatus, gel electrophoresis groove and gel electrophoresis case, described gel electrophoresis case comprises gel electrophoresis box cover and gel electrophoresis case body, gel electrophoresis box cover buckle closure is square with it at gel electrophoresis case, described gel electrophoresis groove is positioned at gel electrophoresis case body, gapped between gel electrophoresis groove and gel electrophoresis case body inwall, both positive and negative polarity interface on gel electrophoresis groove is electrically connected to the both positive and negative polarity interface on gel-electrophoretic apparatus respectively by power lead and the power lead outlet that is positioned at gel electrophoresis case body lower end, described gel electrophoresis case body front is also provided with watch window, in this window inner side, be also provided with a thermometer.
Above-mentioned gel electrophoresis case sheathing material is plastic foam.
Aforesaid watch window material is clear glass.
The beneficial effects of the utility model:
The simple and easy temperature control PCR-SSCP of the utility model gel electrophoresis apparatus, during use, can observe gel electrophoresis situation by the watch window on gel electrophoresis groove, also can understand the temperature in gel electrophoresis case by thermometer, if temperature, not within the scope of requirement of experiment, can be placed ice cube, ice bag etc. by the gap toward between gel electrophoresis groove and gel electrophoresis case and regulate the temperature in gel electrophoresis case; Because gel electrophoresis case shell adopts plastic foam, make, gel electrophoresis case insulating power is strong simultaneously, can make experiment carry out in metastable temperature range.Compared with prior art, the utlity model has simple in structure, convenient and practical, cheap, the advantage that temperature is easy to adjust, overcome existing experimental technique inconvenient operation, price too high, cannot meet the problems such as low temperature deposition condition, promote the use of in the areas such as Ke school, hospital, factory, benefit is obvious.
accompanying drawing explanation:
Fig. 1 is structural representation of the present utility model;
Fig. 2 is vertical view of the present utility model.
embodiment:
Embodiment:
With reference to figure 1 and Fig. 2, the simple and easy temperature control PCR-SSCP of the utility model gel electrophoresis apparatus, comprise gel-electrophoretic apparatus 1, gel electrophoresis groove 8 and gel electrophoresis case, gel electrophoresis case comprises gel electrophoresis box cover 2 and gel electrophoresis case body 3, gel electrophoresis box cover 2 buckle closures are above gel electrophoresis case body 3, gel electrophoresis case shell adopts plastic foam to make, insulating power is stronger, gel electrophoresis groove 8 is arranged in gel electrophoresis case body 3, gap 7 between gel electrophoresis groove 8 and gel electrophoresis case body 3 inwalls is used as temperature adjusting chamber, both positive and negative polarity interface 11 on gel electrophoresis groove 8 is electrically connected to the both positive and negative polarity interface 10 on gel-electrophoretic apparatus 1 respectively by power lead 9 and the power lead outlet 6 that is positioned at gel electrophoresis case body 3 lower ends, right side, gel electrophoresis case body 3 fronts are provided with the watch window 4 that clear glass is made, to observe gel electrophoresis situation, in this window inner side, a thermometer 5 is also installed, so that the temperature in monitoring gel electrophoresis case.
During use, gel electrophoresis offset plate is fixed on gel electrophoresis groove 8, by watch window 4, observes and loadings, if find that experimental temperature is not in claimed range, ice cube, ice bag etc. can be positioned over and in temperature conditioning chamber, regulate the temperature in gel electrophoresis case.
The utility model is applied in low temperature PCR-SSCP gel electrophoresis experiment, have simple in structure, easy to operate, cheap, can regulate and control and monitor the advantages such as gel electrophoresis temperature, observable gel electrophoresis situation.

Claims (3)

1. a simple and easy temperature control PCR-SSCP gel electrophoresis apparatus, comprise gel-electrophoretic apparatus (1), gel electrophoresis groove (8) and gel electrophoresis case, described gel electrophoresis case comprises gel electrophoresis box cover (2) and gel electrophoresis case body (3), gel electrophoresis box cover (2) buckle closure is in gel electrophoresis case body (3) top, it is characterized in that: described gel electrophoresis groove (8) is positioned at gel electrophoresis case body (3), between gel electrophoresis groove (8) and gel electrophoresis case body (3) inwall gapped (7), both positive and negative polarity interface (11) on gel electrophoresis groove (8) is electrically connected to the both positive and negative polarity interface (10) on gel-electrophoretic apparatus (1) respectively by power lead (9) and the power lead outlet (6) that is positioned at gel electrophoresis case body (3) lower end, described gel electrophoresis case body (3) front is also provided with watch window (4), in this window inner side, be also provided with a thermometer (5).
2. simple and easy temperature control PCR-SSCP gel electrophoresis apparatus according to claim 1, is characterized in that: described gel electrophoresis case sheathing material is plastic foam.
3. simple and easy temperature control PCR-SSCP gel electrophoresis apparatus according to claim 1, is characterized in that: described watch window (4) material is clear glass.
CN201320756394.4U 2013-11-27 2013-11-27 Simple temperature control PCR-SSCP gel electrophoresis device Expired - Fee Related CN203551507U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201320756394.4U CN203551507U (en) 2013-11-27 2013-11-27 Simple temperature control PCR-SSCP gel electrophoresis device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201320756394.4U CN203551507U (en) 2013-11-27 2013-11-27 Simple temperature control PCR-SSCP gel electrophoresis device

Publications (1)

Publication Number Publication Date
CN203551507U true CN203551507U (en) 2014-04-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201320756394.4U Expired - Fee Related CN203551507U (en) 2013-11-27 2013-11-27 Simple temperature control PCR-SSCP gel electrophoresis device

Country Status (1)

Country Link
CN (1) CN203551507U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105116042A (en) * 2015-09-25 2015-12-02 贵州大学 PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) gel electrophoresis low-temperature operation box

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105116042A (en) * 2015-09-25 2015-12-02 贵州大学 PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) gel electrophoresis low-temperature operation box

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140416

Termination date: 20151127