CN103202128A - Indoor puritytesting method of glyphosate-resistant cotton seed - Google Patents
Indoor puritytesting method of glyphosate-resistant cotton seed Download PDFInfo
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- CN103202128A CN103202128A CN2013101058471A CN201310105847A CN103202128A CN 103202128 A CN103202128 A CN 103202128A CN 2013101058471 A CN2013101058471 A CN 2013101058471A CN 201310105847 A CN201310105847 A CN 201310105847A CN 103202128 A CN103202128 A CN 103202128A
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Abstract
The invention discloses an indoor purity testing method of glyphosate-resistant cotton seeds, including: step one, break seed dormancy, then divide the seeds into two parts; step two, have the washed and drained yellow sand undergone high temperature sterilization; step three, prepare composite seed coating chemicals(SCC) when required for use; step four, respectively coat the two parts of seeds, dormancy of which have been broken , then conduct the germination test; step five, sow the seeds, and cultivate them under variable temperature for 10 days; step six, determine whether the cotton seedlings are glyphosate-resistant based on two standards including the lengths of seedling roots and whether the hypocotyls of the cotton seedlings have normal elongation growth, if elongation growth of the tap roots and hypocotyls with branched roots and root hairs of the cotton seedlings is normal , the cotton seedings are glyphosate-resistant, otherwise they are not, and the purity test for the glyphosate-resistant cotton seeds is achieved. The invention can complete the purity identification of glyphosate-resistant cotton seeds successfully with reliable results, simple process and short periodic time, which provides high application value.
Description
Technical field
The present invention relates to a kind of authentication method of plant seed purity, be specifically related to a kind of method of indoor detection resistance glyphosate cotton seed purity, belong to field of plant variety breeding technology.
Background technology
At present, for fear of the injury of weed killer herbicide to cotton, carried out the cultivation research of the cotton seeds of antiweed by several different methods, and obtained certain achievement.Because glyphosate has low toxicity, noresidue, and is very effective to the prennical root weeds, and is used widely.But the improper use of glyphosate also can damage cotton, thereby reduces output of cotton.Therefore, the cotton seeds that at present the cultivation research of the cotton seeds of antiweed is mainly concentrated on resistance glyphosate is cultivated.
And after cultivating the cotton seeds of resistance glyphosate, need carry out the cotton seed purity of resistance glyphosate to identify, adopt the evaluation mode of common cultivation at present basically, the time is long, and identification result is poor.
Summary of the invention
The object of the present invention is to provide the method for simple, the effective indoor detection resistance glyphosate cotton seed purity of a kind of mode.
The present invention is achieved in that
A kind of method of indoor detection resistance glyphosate cotton seed purity comprises the steps:
Step 1, seed is put into mesh bag or cloth bag, place in the electric heating air blast thermostatic drying chamber and carry out low-temperature heat, temperature is set at 38 ℃, heats 48 hours, breaks seed dormancy, seed is divided into two parts then;
Step 2, will clean the yellow ground that drains and put into canister and be built in high temperature heating in the angry temperature drying box of electric heating air blast, temperature is set at 200 ℃, 8 hours heat time heating times, carries out high-temperature sterilization;
Step 3, get disease preventing type kind clothing agent 0.1kg, add 41% glyphosate isopropyl amine salt aqua 0.025kg and mix, be mixed with compound seed coating material, with joining with usefulness;
Step 4, with two parts of seeds of breaking dormancy, a ratio that adds the 100g prophylaxis agent for coating seeds according to every 5kg seed is carried out dressing in contrast, another part carries out dressing in the ratio that every 5kg seed adds the 125g compound seed coating material, does germination test behind the dressing;
After step 5, the yellow ground after will sterilizing and an amount of temperature boiling water or pure water are mixed all, the seed of getting behind the step 4 dressing is sowed respectively, each sample is done four repetitions, after planting place and carry out alternating temperature cultivation in 10 days in the incubator, be 12 hours every day in photostage, 30 ℃ of temperature, cultivation that every day is unglazed 12 hours, 28 ℃ of temperature;
Step 6, according to cotton shoot root be length and hypocotyl normally two indexs of elongation growth determine whether resistance glyphosate of cotton seedling, all main roots normally extend, and there is the cotton seedling hypocotyl of supporting root and Gen Mao normally to extend, be the cotton seedling of resistance, otherwise be exactly not anti-cotton seedling, namely finished the evaluation of resistance glyphosate cotton seed purity.
Further scheme is: in the step 5, sowing is to adopt cotton seed germination test box, yellow ground is put into cotton seed germination test box sow then, and the seed amount that each repetition is sowed in cotton seed germination test box is identical.
Among the present invention, adopt yellow ground after the high-temperature sterilization as the sowing medium of seed, raw material sources are very general, and cost is low, and through sterilization, can avoid in the qualification process harmful germ etc. that qualification result is produced harmful effect.In addition, during cultivation, the setting of illumination, temperature is that the inventor obtains through long-term practice, by adopting culture parameters setting of the present invention, can promote cotton seedling growth to greatest extent.The present invention adopts the method for indoor culture identification, can finish the evaluation of resistance glyphosate cotton seed purity smoothly, reliable results, and method is simple, and the cycle is short, has very strong application value.
Embodiment
The method of indoor detection resistance glyphosate cotton seed purity comprises the steps:
Step 1, seed is put into mesh bag or cloth bag, place in the electric heating air blast thermostatic drying chamber and carry out low-temperature heat, temperature is set at 38 ℃, heats 48 hours, breaks seed dormancy, seed is divided into two parts then;
Step 2, will clean the yellow ground that drains and put into canister and be built in high temperature heating in the angry temperature drying box of electric heating air blast, temperature is set at 200 ℃, 8 hours heat time heating times, carries out high-temperature sterilization;
Step 3, get disease preventing type kind clothing agent 0.1kg, add 41% glyphosate isopropyl amine salt aqua 0.025kg and mix, be mixed with compound seed coating material, with joining with usefulness; Described prophylaxis agent for coating seeds is the kind clothing agent with effects such as sterilization, desinsections, for example is the active component that can contain such as Metalaxyl-M, Imidacloprid, Difenoconazole, carbendazim, tmtd, carbofuran etc.
Step 4, with two parts of seeds of breaking dormancy, a ratio that adds the agent of 100g disease preventing type kind clothing according to every 5kg seed is carried out dressing in contrast, another part carries out dressing in the ratio that every 5kg seed adds the 125g compound seed coating material, does germination test behind the dressing;
After step 5, the yellow ground after will sterilizing and an amount of temperature boiling water or pure water are mixed all, the seed of getting behind the step 4 dressing is sowed respectively, each sample is done four repetitions, after planting place and carry out alternating temperature cultivation in 10 days in the incubator, be 12 hours every day in photostage, 30 ℃ of temperature, cultivation that every day is unglazed 12 hours, 28 ℃ of temperature; Sowing is to adopt cotton seed germination test box, yellow ground is put into cotton seed germination test box sow then, and the seed amount that each repetition is sowed in cotton seed germination test box is identical;
Step 6, according to cotton shoot root be length and hypocotyl normally two indexs of elongation growth determine whether resistance glyphosate of cotton seedling, all main roots normally extend, and there is the cotton seedling hypocotyl of supporting root and Gen Mao normally to extend, be the cotton seedling of resistance, otherwise be exactly not anti-cotton seedling, namely finished the evaluation of resistance glyphosate cotton seed purity.
Number of seedling in the foundation contrast cotton seed germination test box and the cotton seedling number of the cotton seedling of resistance calculate the ratio of the cotton seedling of resistance, namely
Claims (2)
1. the method for an indoor detection resistance glyphosate cotton seed purity is characterized in that comprising the steps:
Step 1, seed is put into mesh bag or cloth bag, place in the electric heating air blast thermostatic drying chamber and carry out low-temperature heat, temperature is set at 38 ℃, heats 48 hours, breaks seed dormancy, seed is divided into two parts then;
Step 2, will clean the yellow ground that drains and put into canister and be built in high temperature heating in the angry temperature drying box of electric heating air blast, temperature is set at 200 ℃, 8 hours heat time heating times, carries out high-temperature sterilization;
Step 3, get disease preventing type kind clothing agent 0.1kg, add 41% glyphosate isopropyl amine salt aqua 0.025kg and mix, be mixed with compound seed coating material, with joining with usefulness;
Step 4, with two parts of seeds of breaking dormancy, a ratio that adds the 100g prophylaxis agent for coating seeds according to every 5kg seed is carried out dressing in contrast, another part carries out dressing in the ratio that every 5kg seed adds the 125g compound seed coating material, does germination test behind the dressing;
After step 5, the yellow ground after will sterilizing and an amount of temperature boiling water or pure water are mixed all, the seed of getting behind the step 4 dressing is sowed respectively, each sample is done four repetitions, after planting place and carry out alternating temperature cultivation in 10 days in the incubator, be 12 hours every day in photostage, 30 ℃ of temperature, cultivation that every day is unglazed 12 hours, 28 ℃ of temperature;
Step 6, according to cotton shoot root be length and hypocotyl normally two indexs of elongation growth determine whether resistance glyphosate of cotton seedling, all main roots normally extend, and there is the cotton seedling hypocotyl of supporting root and Gen Mao normally to extend, be the cotton seedling of resistance, otherwise be exactly not anti-cotton seedling, namely finished the evaluation of resistance glyphosate cotton seed purity.
2. according to the method for the described indoor detection resistance glyphosate cotton seed purity of claim 1, it is characterized in that: in the step 5, sowing is to adopt cotton seed germination test box, yellow ground is put into cotton seed germination test box sow then, the seed amount that each repetition is sowed in cotton seed germination test box is identical.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101058471A CN103202128A (en) | 2013-03-19 | 2013-03-19 | Indoor puritytesting method of glyphosate-resistant cotton seed |
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| CN2013101058471A CN103202128A (en) | 2013-03-19 | 2013-03-19 | Indoor puritytesting method of glyphosate-resistant cotton seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108552214A (en) * | 2018-04-10 | 2018-09-21 | 河南省农业科学院 | A kind of resistance glyphosate corn glyphosate coating agent and its application |
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| CN1394472A (en) * | 2001-07-10 | 2003-02-05 | 江苏省农业科学院经济作物研究所 | Transgenic cotton seed purity identification method |
| EP2311856A1 (en) * | 2005-12-09 | 2011-04-20 | BASF Plant Science GmbH | Polypeptides involved in regulation of sugar and lipid metabolism and methods of use VIII |
| CN102165885A (en) * | 2010-12-15 | 2011-08-31 | 中国农业科学院植物保护研究所 | Method for identifying glyphosate resistance of cotton |
| WO2012100963A1 (en) * | 2011-01-28 | 2012-08-02 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Seed trait prediction by activity-based protein profiling |
| CN102919120A (en) * | 2012-12-04 | 2013-02-13 | 江西省棉花研究所 | Breeding method for disease-resistant, insect-resistant and herbicide-resistant cotton variety |
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2013
- 2013-03-19 CN CN2013101058471A patent/CN103202128A/en active Pending
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| CN1394472A (en) * | 2001-07-10 | 2003-02-05 | 江苏省农业科学院经济作物研究所 | Transgenic cotton seed purity identification method |
| EP2311856A1 (en) * | 2005-12-09 | 2011-04-20 | BASF Plant Science GmbH | Polypeptides involved in regulation of sugar and lipid metabolism and methods of use VIII |
| CN102165885A (en) * | 2010-12-15 | 2011-08-31 | 中国农业科学院植物保护研究所 | Method for identifying glyphosate resistance of cotton |
| WO2012100963A1 (en) * | 2011-01-28 | 2012-08-02 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Seed trait prediction by activity-based protein profiling |
| CN102919120A (en) * | 2012-12-04 | 2013-02-13 | 江西省棉花研究所 | Breeding method for disease-resistant, insect-resistant and herbicide-resistant cotton variety |
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| 付志远 等: "利用种子发芽试验检测抗草甘膦棉花新品系", 《种子》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108552214A (en) * | 2018-04-10 | 2018-09-21 | 河南省农业科学院 | A kind of resistance glyphosate corn glyphosate coating agent and its application |
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Application publication date: 20130717 |