CN102919120A - Breeding method for disease-resistant, insect-resistant and herbicide-resistant cotton variety - Google Patents
Breeding method for disease-resistant, insect-resistant and herbicide-resistant cotton variety Download PDFInfo
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- CN102919120A CN102919120A CN2012105113524A CN201210511352A CN102919120A CN 102919120 A CN102919120 A CN 102919120A CN 2012105113524 A CN2012105113524 A CN 2012105113524A CN 201210511352 A CN201210511352 A CN 201210511352A CN 102919120 A CN102919120 A CN 102919120A
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Abstract
The invention provides a breeding method for a disease-resistant, insect-resistant and herbicide-resistant cotton variety, comprising the following step of: by taking bivalence-converted chitinase and glucanase disease-resistant gene cotton as female parent, and bivalence-converted Bt crystal protein insect-resistant gene and 5-enol form pyruvic acid base shikimic acid-3-phosphoric acid synthetase glyphosate-resistant gene cotton as male parent, hybridizing to obtain F1 generation hybrid cotton seeds. The hybrid cotton bred by the breeding method of the cotton with three resistances provided by the invention is high in fusarium vasinfectum resistance and greensickness resistance, wherein the resistance indexes are respectively 2.08 and 1.50; the hybrid cotton is intermediate in cotton bollworm, wherein the resistance index L is less than 30 and greater than or equal to 10; and the hybrid cotton is high in glyphosate resistance, wherein the resistance efficiency is 74.5%. The single boll weight is 6.1g, and the ginning outturn is 43.0%, which are equal to those of the current high-quality hybrid inset-resistant cotton, and the unginned cotton yield is 238.7kg/667m<2>, and the ginned cotton yield is 104.3kg/667m<2>, which are respectively increased by 6.71% and 8.30% compared with the hybrid No.1 Jiangxi cotton of the control variety in the locality.
Description
Technical field
The present invention relates to a kind of three resistant cotton breed breeding methods of disease-resistant, pest-resistant, antiweed.
Technical background
Utilize at present domestic having worked out of genetically modified organism technology to turn Two antidisease genes (Chi+Glu) cotton, turn divalent insect-resistant resistance glyphosate weed killer herbicide gene (Cry1Ac+EPSPS) cotton, and unit price to turn the Bt anti insect gene cotton.These kinds are gone back imperfection in resistance on producing, or disease-resistant not pest-resistant not antiweed, or not disease-resistant (fusarium wilt, verticillium wilt) pest-resistant resistance glyphosate weed killer herbicide only, in addition pest-resistant, the not disease-resistant also glyphosate herbicidal of anti-broad spectrum activity not only.The efficient light Cotton Production pattern of simplifying now requires to work out a kind of both resisting cotton blights, verticillium wilt, again the cotton yield new varieties with three kinds of resistances of bollworm resisting and anti-broad spectrum weeding agent glyphosate.
Summary of the invention
Purpose of the present invention just provides a kind of both resisting cotton blights wither disease, verticillium wilt, the lepidoptera pest such as bollworm resisting again, the simultaneously selection of three resistant cottons of anti-broad spectrum weeding agent glyphosate also.
Three resistant cotton breed breeding methods of disease-resistant, pest-resistant, antiweed of the present invention may further comprise the steps:
1, cotton as maternal to turn bivalent chitinase and dextranase disease-resistant gene (Chi+Glu), turn bivalent Bt crystalline protein anti insect gene and 5-enol pyruvic acid base shikimic acid-3-phosphate synthase Antiglyphosate gene (Cry1Ac+EPSPS) cotton and hybridize for male parent, obtain F1 for the hybrid cotton seed;
2, F1 directly being sowed plantation for seed, what grow is the cotton with three kinds of resistances.
The hybrid cotton high resistance cotton that the selection of three resistant cottons of the present invention is cultivated is withered, verticillium wilt, and anti-finger is respectively 2.08 and 1.50; Middle bollworm resisting, anti-finger 30＞L 〉=10; High-resistance glyphosate, anti-effect 74.5%.The heavy 6.1g of single bell, ginning outturn 43.0%, suitable with present high-quality hybrid Insect Resistant Cotton, seed cotton yield 238.7kg/667m
2, lint yield 104.3kg/667m
2, respectively than cotton assorted No. 1 volume increase 6.71% and 8.30% in contrast kind of Jiangxi, locality.
Embodiment
A kind of three resistant cotton breed breeding methods of disease-resistant, pest-resistant, antiweed comprise the following steps:
1, the parent organizes collection: collect 3 parts of resistance glyphosate materials: the 206(resistance glyphosate), the 207(resistance glyphosate), the anti-potassium deficiency of 208(resistance glyphosate); 1 part of the pest-resistant two anti-material of resistance glyphosate: the 209(resistance glyphosate is pest-resistant); 5 parts of disease-resistant materials: bivalent disease-resistant is cotton, in plant cotton No. 2, in plant cotton No. 6, in plant cotton No. 8, planting cotton No. 5; Many parts of pest-resistant materials: 021,022,023, anti-cotton No. 1 of Jiangxi, anti-cotton No. 3 of Jiangxi, in 50, in 58 etc.; Some parts of other various proterties good quality and high output materials.In the planting experiment ground, observe its economical character, output, quality etc., resistant material is made Resistance Identification simultaneously.
2, resistance glyphosate evaluation and screening: the resistance glyphosate material is used respectively 40 times of liquid Direct sprayings of 10% glyphosate before seedbed and cotton budding, anti-cotton plant is not killed, be tested and appraised each material resistance between 16～65%, dial except dead strain and injured strain, keep normal strain, continue plantation next year, identify again, purify by screening in continuous 4 years, resistance brings up to 80～95%.
3, anti-withered, verticillium wilt evaluation and screening: large Tanaka's conventional seed planting outside the sick garden, feeding block seedlings raising.Cotton seedling is transplanted into respectively during tri-leaf period in cotton withered, the verticillium wilt garden, 4 repetitions of each kind material and contrast (establish Ji cotton 11 and be susceptible contrast, Henan cotton 21 be disease-resistant contrast), randomized arrangement, repetition of every behavior, spacing in the rows 30cm, line-spacing 80cm, cotton 20 strains are planted in every residential quarter.Cotton is withered, verticillium wilt was investigated 1-2 time from the rear every month of falling ill, and indoor species test is produced in theoretical survey in late October.Morbidity strain investigation adopts domestic withered, verticillium wilt to unify grade scale.
4, resistance to insects evaluation and screening: the polyurethane artificial sponge that cuts is inserted the polypropylene plastic box bottom, and with the sealing of scalpel equidistant stroke of three long 2cm on the artificial sponge, after injecting afterwards the 15ml pure water and making artificial sponge's uniform water absorption, with tweezers cotton leaf is uprightly inserted in the box in the sponge sealing, every box is inserted three on leaf, and every leaf connects 2 of newly hatched larvaes, and every box connects 6 of worms, take a box as a repetition, each test material is that a processing is done in three repetitions (three boxes).Give birth to survey culturing room's temperature control at 26-28 ℃, relative moisture is controlled at 70-80%, take natural lighting as periodicity of illumination, connects behind the worm 5 days and checks the borer population of living.
5, combo is closed: take proterties complementary with stable resistant material (resistance glyphosate material, disease-resistant material, pest-resistant material) and other merit material, the principle of mutual supplement with each other's advantages, phase mutual cross, 30 of preparation combinations: be respectively 1, the large peach of Dean * 023; 2,207 * Jiangxi 999; 3,207 * FJA; 4, FJA * 207; 5,207 * 023; 6,023 * 207; 7,208 * 201; 8,201 * 208; 9,208 * Soviet Union is cotton; 10, Soviet Union cotton * 208; 11,208 * 022; 12,022 * 208; 13,208 * anti-3; 14, anti-3 * 208; 15,208 * 205; 16,205 * 208; 17,209 * Jiangxi 11; 18, Jiangxi 11 * 209; 19,209 * 023; 20,209 * 022; 21,209 * bivalent disease-resistant is cotton; 22, bivalent disease-resistant cotton * 209; 23,209 * FJA; 24, FJA * 209; 25,209 * Soviet Union is cotton; 26, Soviet Union cotton * 209; 27, the large peach of 209 * Dean; 28, the large peach of Dean * 209; 29,209 * LKS; 30, LKS * 209.
6, the advantage combination production of hybrid seeds and advantage are measured: the experimental field plantation that is combined in of preparation, carrying out advantage measures, reasonable combination has: 207 * 023,208 * and anti-3,209 * Jiangxi 11, Jiangxi 11 * 209,209 * bivalent disease-resistant is cotton, bivalent disease-resistant is cotton * 209, the large peach of 209 * Dean etc., the combination of will do very well, resistance is stable repeats combo, measure again, and increase new combo and close.
7, transgressive inheritance test: with the Its Reciprocal Hybrids of the pest-resistant material 209 of resistance glyphosate and bivalent disease-resistant material formulation (209 * bivalent disease-resistant is cotton, bivalent disease-resistant is cotton * 209), do together super close test with its parent (209, bivalent disease-resistant cotton) and contrast kind of (Jiangxi is cotton mixes No. 1), the result not only surpasses parent and contrast at yield and quality, have superiority, and resistance glyphosate, the proterties such as disease-resistant, pest-resistant also can preserve, and is more satisfactory how anti-(3 is anti-) hybrid cotton.
8, best three anti-cotton F1 the selection result: advantage three anti-hybrid combinations screening F1 crossbreed: disease-resistant, antiweed, bollworm-resistance hybrid cotton test: process 1, the resistance glyphosate Insect Resistant Cotton; 2, disease-resistant cotton; 3, resistance glyphosate Insect Resistant Cotton * disease-resistant cotton F1; 4, disease-resistant cotton * resistance glyphosate Insect Resistant Cotton F1; 5, Jiangxi cotton assorted No. 1 (Insect Resistant Cotton ck) carries out the advantage comparative measurements with above-mentioned kind and hybrid combination in plantation experimental field, and situation is as follows:
Growing process:
Economical character:
The species test result:
| Numbering | Dan Lingchong | Ginning outturn | Son refers to | Clothing refers to | Boll is heavy |
| 1 | 5.4 | 41.5 | 10.8 | 7.8 | 2.0 |
| 2 | 5.2 | 41.6 | 10.4 | 7.4 | 1.8 |
| 3 | 5.9 | 43.0 | 11.1 | 8.3 | 1.9 |
| 4 | 6.1 | 43.7 | 10.8 | 8.3 | 2.1 |
| 5ck | 5.6 | 43.1 | 11.6 | 8.4 | 1.9 |
Residential quarter unginned cotton's output (area: 0.03 mu):
Residential quarter lint yield (area: 0.03 mu):
Investigation on May 20 resistance glyphosate effect:
Annotate: May 13,40 times of liquid of 10% glyphosate were sprayed in the seedbed.
Anti-withered, verticillium wilt disease index
:
| Kind | Fusarium wilt disease index (investigation on June 23) | Verticillium wilt disease index (investigation on July 8) |
| 1 | 5.58 | 6.23 |
| 2 | 1.75 | 1.33 |
| 3 | 1.92 | 2.67 |
| 4 | 2.08 | 1.50 |
| 5CK | 8.33 | 9.25 |
Pest-resistant effect:
| Kind | Resistance to insects |
| 1 | Anti- |
| 2 | Sense |
| 3 | Anti- |
| 4 | In anti- |
| 5CK | Anti- |
The fiber quality testing result:
The result: mensuration filters out to turn bivalent chitinase (Chi) and dextranase (Glu) disease-resistant gene is cotton for maternal, turn bivalent Bt crystalline protein (Cry1Ac) anti insect gene and 5-enol pyruvic acid base shikimic acid-3-phosphate synthase (EPSPS) Antiglyphosate gene cotton and hybridize (kind 4) for male parent, the Hybrid F1 of cultivating is on behalf of best three anti-hybrid cotton kinds.F1 is directly sowed plantation for seed, and what grow is the cotton with three kinds of resistances.
Claims (1)
1. three resistant cotton breed breeding methods of disease-resistant, pest-resistant a, antiweed, it is characterized in that: it may further comprise the steps:
(1), to turn bivalent chitinase and dextranase disease-resistant gene cotton as maternal, turn bivalent Bt crystalline protein anti insect gene and 5-enol pyruvic acid base shikimic acid-3-phosphate synthase Antiglyphosate gene cotton and hybridize for male parent, obtain F1 for the hybrid cotton seed;
(2), F1 directly being sowed plantation for seed, what grow is the cotton with three kinds of resistances.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202128A (en) * | 2013-03-19 | 2013-07-17 | 盐城市育新种业有限公司 | Indoor puritytesting method of glyphosate-resistant cotton seed |
| CN104012398A (en) * | 2014-06-13 | 2014-09-03 | 中国农业科学院棉花研究所 | Method for screening high-glyphosate-resistant semi-wild cotton material |
| CN106520823A (en) * | 2016-09-29 | 2017-03-22 | 湖南省棉花科学研究所 | Culture method of transgenic insect-resistant new cotton line |
| CN106538373A (en) * | 2016-09-29 | 2017-03-29 | 湖南省棉花科学研究所 | A kind of breeding method of Transgenic Resistant Herbicide, high temperature resisting cotton new lines |
| CN112470919A (en) * | 2020-12-18 | 2021-03-12 | 四川省农业科学院经济作物育种栽培研究所 | Method for breeding new transgenic insect-resistant and glyphosate-resistant cotton strain |
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| CN1373992A (en) * | 2002-04-28 | 2002-10-16 | 南京农业大学 | Breeding process of transgenic bollworm resisting cotton hybrid |
| CN1375188A (en) * | 2001-03-20 | 2002-10-23 | 浙江大学 | Breeding process of three-line transgenic cotton hybrid |
| CN102119654A (en) * | 2010-11-29 | 2011-07-13 | 山东冠丰种业科技有限公司 | Method for breeding new variety of insect-resistant cotton |
| US20110225670A1 (en) * | 2010-03-11 | 2011-09-15 | Monsanto Technology Llc | Cotton variety 09r605b2r2 |
| US20120233721A1 (en) * | 2012-05-18 | 2012-09-13 | House Of Agriculture Spirou Aebe | Cotton Cultivar L-1851 |
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| CN1375188A (en) * | 2001-03-20 | 2002-10-23 | 浙江大学 | Breeding process of three-line transgenic cotton hybrid |
| CN1373992A (en) * | 2002-04-28 | 2002-10-16 | 南京农业大学 | Breeding process of transgenic bollworm resisting cotton hybrid |
| US20110225670A1 (en) * | 2010-03-11 | 2011-09-15 | Monsanto Technology Llc | Cotton variety 09r605b2r2 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202128A (en) * | 2013-03-19 | 2013-07-17 | 盐城市育新种业有限公司 | Indoor puritytesting method of glyphosate-resistant cotton seed |
| CN104012398A (en) * | 2014-06-13 | 2014-09-03 | 中国农业科学院棉花研究所 | Method for screening high-glyphosate-resistant semi-wild cotton material |
| CN104012398B (en) * | 2014-06-13 | 2016-05-04 | 中国农业科学院棉花研究所 | The method of screening high-resistance glyphosate semi-wild cotton material |
| CN106520823A (en) * | 2016-09-29 | 2017-03-22 | 湖南省棉花科学研究所 | Culture method of transgenic insect-resistant new cotton line |
| CN106538373A (en) * | 2016-09-29 | 2017-03-29 | 湖南省棉花科学研究所 | A kind of breeding method of Transgenic Resistant Herbicide, high temperature resisting cotton new lines |
| CN112470919A (en) * | 2020-12-18 | 2021-03-12 | 四川省农业科学院经济作物育种栽培研究所 | Method for breeding new transgenic insect-resistant and glyphosate-resistant cotton strain |
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Application publication date: 20130213 |