CN106973786B - A kind of anti-head-smut Sorghum Breeding method - Google Patents
A kind of anti-head-smut Sorghum Breeding method Download PDFInfo
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- CN106973786B CN106973786B CN201710306923.3A CN201710306923A CN106973786B CN 106973786 B CN106973786 B CN 106973786B CN 201710306923 A CN201710306923 A CN 201710306923A CN 106973786 B CN106973786 B CN 106973786B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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Abstract
A kind of anti-silk dust-brand Sorghum Breeding method, belong to agriculture rearing new variety field, its step includes being combined to be maternal by seedling identification in hybridization, the anti-silk dust-brand hybrid Population room of the means being selfed creation and the identification of field strain with anti-silk dust-brand sorghum strain, realize the breeding method of generation Resistance Identification selection twice, rapidly and accurately the anti-silk dust-brand sorghum variety of breeding.The anti-head-smut strain that the present invention is bred as can be used as the male parent or maternal breeding hybridized for anti-head-smut of sorghum hybrid, the anti-head-smut sorghum of incubation, the situation that upper sorghum field head smut occurs on a large scale that produces is avoided, achievees the purpose that increasing both production and income.
Description
Technical field
The invention belongs to agriculture field, especially anti-silk dust-brand Sorghum Breeding method.
Background technique
Head smut is one of the Major Diseases for influencing the development of China's sorghum production, has generation every year in sorghum producing region,
Caused harm with northeast and North China the most serious, disease incidence is sometime up to 70%, causes very big loss to production.Production practices
Prove, had using medicament or cultivation step prevention and treatment should not carry out, not enough economy, control efficiency are undesirable and cause environmental pollution etc.
Limitation.Studies at home and abroad show that the major measure for preventing and treating the disease is plantation disease-resistant variety.The anti-silk dust-brand kind of breeding sorghum has
Necessity invents anti-silk dust-brand Sorghum Breeding method, currently, disease-resistant test is mainly field test, when single field test exists
Field area is big, needs the problems such as time is long, working efficiency is low.
Summary of the invention
It is big in order to which there is field areas when solving the problems, such as anti-head-smut identification, need the time long, ineffective,
The present invention provides a kind of anti-head-smut Sorghum Breeding method, and specially field test and indoor growing cone identifies one combined
Kind method, it is quick, accurate to have many advantages, such as.
Technical solution of the present invention: a kind of anti-head-smut Sorghum Breeding method, it is characterised in that with anti-silk dust-brand sorghum
Strain is that female parent passes through hybridization, selfing creates anti-silk dust-brand hybrid Population, and fights a dust-brand hybrid Population progress indoor growing
Cone identification and the identification of field strain.
A kind of anti-head-smut Sorghum Breeding method, includes the following steps:
(1) preparation of anti-head-smut combination obtains F1 seed
Hybridized with the strong material R111 of the anti-head-smut and material L17 of excellent character, harvests Hybrid F1 seed.
(2) foundation of anti-silk dust-brand sorghum group
With Hybrid F1 generation kind in field, it was selfed for 2 generations, harvest F2, F3 is anti-head-smut group for seed.
(3) identification of anti-silk dust-brand sorghum single plant
F2, F3 are for seed, and continuous to be selfed for 2 generations, progress field anti-disease enzyme selects disease-resistant single plant, harvest F4 for seed.
(4) identification of anti-silk dust-brand sorghum strain
It is combined by the identification of indoor growing cone and field test and F4 generation is identified twice, eliminate the high strain of disease incidence
System, selects disease incidence in 10% strain below.
(5) acquisition of anti-silk dust-brand sorghum strain
The strain seed that step (4) are obtained repeats the identification method of step (4), until by the agronomy for the strain being selected in
As anti-head-smut sorghum strain when the basicly stable and anti-silk dust-brand disease incidence of shape is below 10%.
Step (4) the anti-head-smut identification method, the specific steps are as follows:
A. indoor growing cone identification
As unit of anti-head-smut strain of the F4 for seed, 100 seeds are taken on each fringe, carry out resistance mirror indoors
Fixed, with 0.5%(mass percent) bacterium soil (i.e. the sick fringe bacterium powder of the black disease of silk: fine earth=0.5:95.5) be seeded in and cultivate in box,
30 days after emergence, take seedling peel off siphonal lobe, leave growth cone, with aniline blue lactic acid staining reagent (formula: aniline blue 0.1g,
50 milliliters of lactic acid, 100 milliliters of water), microscopy growth of seedling bores base portion separate living tissue, and whether there is or not be dyed to navy blue mycelia.Select seedling
Disease incidence is in 10% strain below, as Resistant variants.
B. field test
By bacterium soil (i.e. the sick fringe bacterium powder of the black disease of silk: fine earth=0.5:95.5) plastic cloth covering 5-7 days.Using bunch planting
Method, when sowing, is first sowed by every cave 2-3, after cover bacterium 100 g of soil again, finally cover field soil again and tread.It is maturity period, high
After the completely aobvious disease of fine strain of millet head smut, plant disease incidence is investigated, selects disease incidence in 10% strain below, as Resistant variants.
Of the invention has the advantage that compared with existing breeding method
(1) for the present invention with the strong material R111 of anti-head-smut as female parent, R111 exempts from 1,2,4 biological strain of silk dust-brand
Epidemic disease, to No. 3 biological strain highly resistances, and other economical characters are also preferable, easily select ideal anti-head-smut strain.
(2) present invention is not influenced using indoor seedling growth cone identification, indoor identification by factors such as seasonal climates, and
Early, not anti-head-smut strain accurately has been eliminated, has reduced field breeding population, improves breeding efficiency.
(3) present invention is combined using the selection of indoor and field, a generation identify twice, the breeding method of selection twice, can
Strong operability accelerates the breeding speed of resistance strain.
The anti-head-smut strain that the present invention is bred as can be used as the male parent or maternal for anti-head-smut of sorghum hybrid
Breeding hybridized, the anti-head-smut sorghum of incubation avoids the situation that upper sorghum field head smut occurs on a large scale that produces,
Achieve the purpose that increasing both production and income.
The strong material of the anti-head-smut refer to sorghum by its group's head smut disease incidence after soil inoculation 10% with
Under highly resistance strain or disease incidence 0 immune strain.
The material of the excellent character refers to several Xiang Zhibiao of all or part: high yield, high-quality, anti-for meeting Sorghum Breeding target
Property good, long-rhizome, push up strong strong native power, deep seeding tolerance, resistance to dense planting, photosynthesis characteristics, anti-abortion, the property such as hybrid vigour is strong, coordinate force is strong
The good strain of shape.
Detailed description of the invention
Fig. 1 is anti-head-smut Sorghum Breeding technology path flow chart.
Specific embodiment
The present invention is described in more detail below in conjunction with specific embodiment, R111, L17 come in the application
Material is saved from Sorghum Research Institute, Shanxi Academy of Agricultural Sciences, wherein test makees resistance mirror with No. 3 biological strains of head smut
Fixed, R111 shows as being immunized to No. 3 biological strains of head smut, and L17 is the sorghum backbone system in research and production.
Embodiment 1:R111*L17 breeding
R111 and L17 hybridization, obtains F1 seed, and by the F1 generation seed kind of acquisition in field, continuous plantation two generations of selfing are obtained
F3 is obtained for seed, two generation seeds all select anti-head-smut single plant, harvest the seed in F4 generation.
The anti-head-smut in indoor identification F4 generation: as unit of the resistance list fringe in F4 generation, every fringe takes 100 seeds in temperature
25 DEG C, the indoor carry out Resistance Identification of relative humidity 70%.
It by sorghum seeds kind in the culture box of dress sandy soil, is inoculated with 0.5% bacterium soil, inserts card mark source.30 after emergence
It, takes seedling to peel off siphonal lobe, leaves growth cone, and with aniline blue lactic acid staining reagent, microscopy growth of seedling bores mitogenetic group of base portion
It is woven with nothing and is dyed to navy blue mycelia.Select seedling head smut disease incidence in 10% strain below, as Resistant variants.
Resistant variants kind in field, field anti-disease enzyme eliminates not Resistant variants, repeats above-mentioned evaluation program, until
In F8 generation, economical character and the anti-head-smut for being selected in single plant are basicly stable, are set to new anti-silk according to the field of its offspring number
Smut stablizes sorghum strain.
F1 is first-filial generation in the present invention；
F2 is second filial generation；
F3 is hybridization three generations；
F4 is four generations of hybridization；
F5 is five generations of hybridization；
F6 is six generations of hybridization；
F7 is seven generations of hybridization；
F8 is eight generations of hybridization.
The partial size of heretofore described fine earth is not more than 0.075mm.
Claims (8)
1. a kind of anti-head-smut Sorghum Breeding method, it is characterised in that the following steps are included:
(1) the strong material R111 of the anti-head-smut and material L17 of excellent character is hybridized, harvests Hybrid F1 seed；
(2) Hybrid F1 generation kind was selfed for 2 generations in field, harvests F3 for seed, two generation seeds all select anti-head-smut list
Strain harvests the seed in F4 generation；
(3) indoor growing cone identification is carried out for seed to F4, selects head smut disease incidence in 10% strain seed below, then
Field test is carried out, not anti-head-smut strain is eliminated；
(4) above-mentioned evaluation program is repeated, until F8 generation, economical character and the anti-head-smut for being selected in single plant are basicly stable, the strain
System is anti-head-smut sorghum strain.
2. a kind of anti-head-smut Sorghum Breeding method according to claim 1, it is characterised in that: the indoor growing cone
Identification method is: as unit of anti-head-smut strain of the F4 for seed, 100 seeds is taken on each fringe, are seeded in bacterium soil
It cultivates in box, 30 days after emergence, takes seedling to peel off siphonal lobe, leave growth cone, growth cone is dyed with aniline blue lactic acid coloring agent,
Whether there is or not be dyed to navy blue mycelia for microscopy growth cone base portion separate living tissue；Select seedling disease incidence in 10% strain below, as
Resistant variants.
3. a kind of anti-head-smut Sorghum Breeding method according to claim 1, it is characterised in that: the field test side
Method is: bacterium soil plastic cloth is covered 5-7 days；Using the method for bunch planting, every cave 2-3, when sowing, first sow, after cover again
Bacterium 100 g of soil, finally cover field soil again and tread；Maturity period after head smut of sorghum shows disease completely, investigates plant disease incidence,
Select disease incidence in 10% strain below, as Resistant variants.
4. a kind of anti-head-smut Sorghum Breeding method according to claim 2, it is characterised in that: the aniline blue lactic acid
Dye agent prescription are as follows: aniline blue 0.1g, 50 milliliters of lactic acid, 100 milliliters of water.
5. a kind of anti-head-smut Sorghum Breeding method according to claim 2, it is characterised in that: the bacterium soil formula is
Mass percent is the sick fringe bacterium powder of head smut: fine earth=0.5%:95.5%.
6. a kind of anti-head-smut Sorghum Breeding method according to claim 2, it is characterised in that the indoor growing cone
Identify that temperature is 25 DEG C, relative humidity is 70%.
7. a kind of anti-head-smut Sorghum Breeding method according to claim 1, it is characterised in that anti-head-smut is stablized
The grade of strain includes immune strain, highly resistance strain.
8. a kind of anti-head-smut Sorghum Breeding method according to claim 7, it is characterised in that the immune strain is
100% single plant seedling does not have head smut；The highly resistance strain is that 90% or more single plant seedling does not have head smut.
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| CN201710306923.3A CN106973786B (en) | 2017-05-04 | 2017-05-04 | A kind of anti-head-smut Sorghum Breeding method |
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| CN201710306923.3A CN106973786B (en) | 2017-05-04 | 2017-05-04 | A kind of anti-head-smut Sorghum Breeding method |
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| CN106973786B true CN106973786B (en) | 2019-10-08 |
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| CN111500470A (en) * | 2020-04-20 | 2020-08-07 | 山西省农业科学院高粱研究所 | Propagation method for different physiological races of sorghum head smut bacteria |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824479A (en) * | 2010-05-07 | 2010-09-08 | 沈阳师范大学 | SCAR markerer of sorghum head smut resistance germ No. 3 physiological strain |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824479A (en) * | 2010-05-07 | 2010-09-08 | 沈阳师范大学 | SCAR markerer of sorghum head smut resistance germ No. 3 physiological strain |
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| 抗高粱丝黑穗病3号小种的品系鉴选与利用研究;赵淑坤等;《国外农学—杂粮作物》;19971231(第2期);第39页右栏最后一段 * |
| 玉米丝黑穗病及病菌生理分化研究进展;贺字典;《玉米科学》;20051231;第13卷(第4期);第117-120,131页 * |
| 玉米品种对丝黑穗病的抗病性和幼苗诊断的研究;刘钖若等;《植物保护学报》;19831231;第10卷(第4期);第275页第2-3段和最后一段、第276页第1-2段 * |
| 高抗高粱丝黑穗病品种晋杂103的选育;田承华等;《中国种业》;20101231(第9期);全文，尤其是左栏第3段 * |
| 高粱幼苗感染丝黑穗病的鉴别技术及系抚侵染过程的观察;白金铠等;《植物病理学报》;19800630;第10卷(第1期);摘要和第39页最后一段到第40页第1段 * |
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