CN103168680A - Method for rapidly and selectively breeding restoring line of hybrid rice - Google Patents
Method for rapidly and selectively breeding restoring line of hybrid rice Download PDFInfo
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- CN103168680A CN103168680A CN2013101291705A CN201310129170A CN103168680A CN 103168680 A CN103168680 A CN 103168680A CN 2013101291705 A CN2013101291705 A CN 2013101291705A CN 201310129170 A CN201310129170 A CN 201310129170A CN 103168680 A CN103168680 A CN 103168680A
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Abstract
The invention discloses a method for rapidly and selectively breeding a restoring line of hybrid rice. The method comprises the steps of: (1) hybridizing F1 generation; (2) determining the restoring capacity of F2 generation; (3) implementing screening based on rice blast resistance on F3 generation and identifying and screening bacterial blight resistance of F4 generation; and (4) identifying and screening hybrid vigour to select strain lines with strong hybrid vigour and consistent characteristics, implementing mixed harvesting breeding, thereby achieving the purpose of rapidly breeding the restoring line. The method has the beneficial effects of: determining that all selected materials contain strong restoring genes in early generation, and greatly improving the accuracy and the success power of the selective breeding of the restoring line since the restoring genes can not be lost due to generational selection. Compared with a conventional breeding method, The method has the advantages that 2-3 generations are ahead of time, and therefore, the breeding time is shortened by 1-2 years; and the restoring line of the hybrid rice can be rapidly bred, so that a great amount of human resources and financial resources are saved and a big effect of cost saving is achieved.
Description
Technical field
The invention belongs to agricultural technology field, more specifically, relate to a kind of method of hybrid rice restorer line seed selection.
Background technology
Hybrid rice produces by different seed rice phase mutual crosses, dependence be that the hybridization of male sterile line (female parent) and restorer (male parent) produces super parents' advantage, reach the purpose of high yield.And to carry out hybrid rice production, and just must select the strong parent of hybrid vigour, restorer is exactly one of parent.The seed selection of restorer and improvement, can be by the method for hybridization, enlarge genetic diversity, thereby fully play the hybrid vigour of hybrid rice, producing at present the upper restorer of promoting is all that extensive selection cross forms basically, the time of extensive selection cross is short, but hereditary difference is little, and a large amount of outstanding conventional Rices is arranged now, but lacks strong recovery gene, can not use restorer, fully excavate the beneficial gene of conventional Rice, be used for the restorer of hybrid rice, enlarge the affiliation of restorer, with the male sterile line combo, better bring into play the hybrid vigour of hybrid rice again.Import conventional Rice and will recover now gene, select time is long, also might lose the recovery gene, causes the seed selection failure.
Summary of the invention
The present invention mainly for be will recover gene in prior art to import conventional Rice, select time is long, also might lose the recovery gene, causes the problem of seed selection failure, provide a kind of breed fast have the conventional Rice blood relationship, and restorer that can use on producing.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method of quick breeding hybrid rice restorer line is characterized in that comprising the following steps:
(1) hybridization: obtain F1 for seed with conventional Rice and the hybridization of strong restorer, F1 is for seed in plantation, mixed gather in the crops F2 for seed.
(2) survey extensive: at F2 generation and male sterile line test cross commonly used, purpose is to select strain material with strong recovery capability, gathers in the crops simultaneously corresponding F3 for seed.
Further, carry out (2) survey extensive after, carry out the blast resisting evaluation and screening.
Further, carry out (2) survey extensive after, carry out the bacterial blight screening.
Further, carry out (2) survey extensive after, first carry out the blast resisting evaluation and screening, then carry out the bacterial blight screening.
Further, after carrying out the bacterial blight screening, then carry out the hybrid vigour screening.
Further, the method of described blast resisting evaluation and screening is: F3 is for seed minute strain plantation, plant simultaneously corresponding test cross seed, the material artificial infection rice blast pathogen that divides the strain plantation, select in having the strain of recovery capability that the good individual plant of blast resisting, the leaf attitude of strain is mixed to be received, obtain F4 for seed.
Further, the method of described bacterial blight screening is: mixed seeding F4 is for seed, artificial infection bacterial leaf-blight germ, select bacterial blight-resisting, the leaf attitude fine individual plant of strain and male sterile line test cross, maturing stage is carried out primary screening again, remove the poor individual plant of bacterial blight-resisting, F5 is for seed and corresponding test cross seed for results.
Further, described hybrid vigour screening technique is: minute strain sowing F5 selects the strong corresponding F5 of test cross kind hybrid vigour for mixed receipts of the consistent strain of feature for seed and corresponding test cross seed, breeds restorer.
The invention has the beneficial effects as follows:
1, F2 is extensive for surveying: the material of early determining to contain strong recovery gene.And the conventional herd breeding method is just to begin to survey extensive for the left and right at F5, according to the conventional herd breeding method, guarantee to recover gene not lose because of selection, just necessarily require colony large, increased like this sowing quantity, need the area of plantation large, expended a large amount of financial resources and manpower, working strength is large; Survey extensive also might causing because screening recovers the risk that gene is lost at Gao Daicai in addition, thereby cause the seed selection failure.The method has just determined that selected materials all contains strong recovery gene in generation early, can be because of selection from generation to generation the loss recovery gene, the accurate power that has greatly improved the restorer seed selection with become power, save a large amount of manpower, financial resources, reached the cost saving effect grand.
2, F3 is for carrying out the blast resisting evaluation and screening, F4 is for carrying out the bacterial blight screening: rice blast is one of important disease of paddy rice, each evenly generation of rice district of the world, this disease all has generation in various places, wherein occur as many with leaf section, fringe neck, can cause the underproduction in various degree after generation, especially panicle blast, can cause spike of rice to form sky paddy or not plump paddy so that total crop failure; At first bacterial blight of rice 19th-century end is found in Japan, has become the important disease in Rice In Asian Cultivated Rice district at present.In China, Japan, Korea, Philippine, India and other countries in Southeast Asia, generation is arranged all, especially region following the line of the sea, the easiest large tracts of land generation bacterial leaf-blight, after rice pathogenesis, cause that generally blade is withered, unreal rate increases, rice matter is crisp, thousand kernel weight reduces, the general underproduction 10-20%, and the serious underproduction is more than 50%; Dead Cong Xianxiang is often caused in the rice field that wilting type bacterial leaf-blight occurs, and loss is serious.The present invention had both carried out the blast resisting inoculated identification, also carried out the bacterial blight-resisting inoculated identification, had avoided general breeder only to carry out rice blast evaluation or bacterial leaf-blight evaluation, even do not carry out the disease inoculated identification; And the present invention carries out inoculated identification in generation morning, utilization takes full advantage of resistant gene, be unlikely to because thinking that screening is lost resistant gene, and at F3, F4 is for just having determined that selected materials contains resistant gene, realized that the seed selection of restorer disease resistance and strong advantage seed selection develop simultaneously, this is that the conventional herd breeding method is not accomplished.
3, F5 is for measuring hybrid vigour:, shifted to an earlier date 2-3 than general conventional breeding and shortened breeding time 1-2 from generation to generation for just having carried out heterotic mensuration at F5, reached quick improved crossing rice restorer.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment
1, hybridize to get F1: utilize artificial emasculation, after the conventional Rice artificial emasculation, award the pollen of strong restorer, the hybridization combo obtains F1 generation, plants F1 generation, the mixed sub-F2 of sowing.
2, F2 is for measuring recovery capability: according to genetics principle, F2 is for producing natural separation, the F2 that sowing mixes is for seed, the select target type is strong for tillering from the plant that separates, big panicle many grains per panicle, proper height, cane is sturdy, the leaf good individual plant of strain and male sterile line hybridization, the results seed, corresponding restorer is also gathered in the crops separately.
3, F3 identifies for blast resisting: F3 plants for plant division, carries out the rice blast artificial infection and identifies, plants simultaneously corresponding generation kind, selects by force to rice blast resistance, and the outstanding individual plant of the strain that recovery capability is strong is mixed to be received, and F4 is for seed in acquisition.
4, F4 is for bacterial blight: F4 is for mixed seeding, artificial infection bacterial leaf-blight germ selects bacterial blight-resisting, the leaf attitude fine individual plant of strain and a plurality of male sterile line test cross, and the maturing stage is carried out primary screening again, remove the poor individual plant of bacterial blight-resisting, F5 is for seed and corresponding test cross seed for results
5, F5 selects for hybrid vigour: a minute strain is broadcast F5 for seed and corresponding test cross seed, selection hybrid vigour is strong, the mixed receipts of strain that feature is consistent, breed new restorer, simultaneously carrying out a plurality of male sterile lines is measured again, advantage is strong, little system is carried out in the cooperation of increase yield significantly, becomes new varieties through participating in regional test above the provincial level after qualified.
Experimental example: seed selection gets extensive 988
Hainan in 2007 gets extensive 529 as maternal take strong restorer, and the conventional Rice of (recovery capability is very weak, long fringe, rice blast resistance is strong) is the male parent conventional hybridization to be numbered 0988, gathers in the crops F1 for seed.
Remove pseudostationary 3 strains in Deyang plantation 21 strains in 2008, and mixed the seed of results 18 strains, obtain F2 for seed.
Winter in 2008 for seed, was selected outstanding individual plant and male sterile line assembly at Hainan plantation F2, gathered in the crops respectively individual plant and obtained F3 for seed and corresponding generation kind seed.
2009 at Deyang plantation F3 for individual plant and corresponding generation kind seed, and carry out the blast resisting inoculated identification, select generation kind ripening rate high during results, the corresponding strain that the male parent rice blast resistance is strong is mixed gather in the crops F4 for seed.
Summer in 2010 is in Hainan mixed seeding, carry out the bacterial blight-resisting inoculated identification, the selection bacterial leaf spot resistance is strong, the individual plant that the leaf attitude of strain is good and male sterile line test cross, maturing stage screens again, select good individual plant F5 generation of results bacterial leaf spot resistance and corresponding test cross seed, eliminate bad individual plant and the test cross seed of resistance of test cross.
Planted F5 in 2011 in the Deyang for seed and corresponding test cross seed, select in the generation kind maturing stage that hybrid vigour is strong, output is high, corresponding strain feature is mixed receipts of strain of neat and consistent also, carries out next step research, new restorer is bred, name extensive 988.Become new varieties through participating in regional test above the provincial level after qualified.
Claims (8)
1. the method for a quick breeding hybrid rice restorer line, its feature comprises the following steps:
(1) hybridization: obtain F1 for seed with conventional Rice and the hybridization of strong restorer, F1 is for seed in plantation, mixed gather in the crops F2 for seed.
(2) survey is extensive: the F2 that sowing mixes is for seed, and individual plant and the male sterile line test cross commonly used of select target type from the plant that separates are gathered in the crops respectively individual plant and obtained F3 for seed and corresponding generation kind seed.
2. the method for a kind of quick breeding hybrid rice restorer line according to claim 1 is characterized in that: carry out step (2) survey extensive after, carry out the blast resisting evaluation and screening.
3. the method for a kind of quick breeding hybrid rice restorer line according to claim 1 is characterized in that: carry out step (2) survey extensive after, carry out the bacterial blight screening.
4. the method for a kind of quick breeding hybrid rice restorer line according to claim 1 is characterized in that: carry out step (2) survey extensive after, first carry out the blast resisting evaluation and screening, then carry out the bacterial blight screening.
5. the method for a kind of quick breeding hybrid rice restorer line according to claim 4, is characterized in that: after carrying out the bacterial blight screening, then carry out the hybrid vigour screening.
6. the method for the described a kind of quick breeding hybrid rice restorer line of any one according to claim 2-5, it is characterized in that: the method for described blast resisting evaluation and screening is: F3 is for seed minute strain plantation, plant simultaneously corresponding test cross seed, the material artificial infection rice blast pathogen that divides the strain plantation, select in having the strain of recovery capability that the good individual plant of blast resisting, the leaf attitude of strain is mixed to be received, obtain F4 for seed.
7. the method for the described a kind of quick breeding hybrid rice restorer line of any one according to claim 3-5, it is characterized in that: the method for described bacterial blight screening is: mixed seeding F4 is for seed, artificial infection bacterial leaf-blight germ, select bacterial blight-resisting, the leaf attitude fine individual plant of strain and male sterile line test cross, maturing stage is carried out primary screening again, remove the poor individual plant of bacterial blight-resisting, F5 is for seed and corresponding test cross seed for results.
8. the method for a kind of quick breeding hybrid rice restorer line according to claim 5, it is characterized in that: described hybrid vigour screening technique is: minute strain sowing F5 is for seed and corresponding test cross seed, select the strong corresponding F5 of test cross kind hybrid vigour for mixed receipts of the consistent strain of feature, breed restorer.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108476969A (en) * | 2018-02-12 | 2018-09-04 | 贵州省农作物品种资源研究所 | Rice homogeneity oozes the selection of round-grained rice restorer |
| CN116210585A (en) * | 2022-08-26 | 2023-06-06 | 海南省农业科学院粮食作物研究所 | Method for molecular marker assisted selection of Hainan disease and pest resistant rice restorer line |
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| US20120240285A1 (en) * | 2011-03-18 | 2012-09-20 | Honda Motor Co., Ltd. | Method for producing rice f1 seed, rice f1 seed, and rice male sterile line |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108476969A (en) * | 2018-02-12 | 2018-09-04 | 贵州省农作物品种资源研究所 | Rice homogeneity oozes the selection of round-grained rice restorer |
| CN116210585A (en) * | 2022-08-26 | 2023-06-06 | 海南省农业科学院粮食作物研究所 | Method for molecular marker assisted selection of Hainan disease and pest resistant rice restorer line |
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Denomination of invention: Method for rapidly and selectively breeding restoring line of hybrid rice Effective date of registration: 20170919 Granted publication date: 20141126 Pledgee: Sichuan Tianfu bank Limited by Share Ltd Deyang branch Pledgor: Sichuan Deyue Technology Seed Industry Co., Ltd. Registration number: 2017510000063 |
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Date of cancellation: 20181016 Granted publication date: 20141126 Pledgee: Sichuan Tianfu bank Limited by Share Ltd Deyang branch Pledgor: Sichuan Deyue Technology Seed Industry Co., Ltd. Registration number: 2017510000063 |
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Denomination of invention: Method for rapidly and selectively breeding restoring line of hybrid rice Effective date of registration: 20181018 Granted publication date: 20141126 Pledgee: Sichuan Tianfu bank Limited by Share Ltd Deyang branch Pledgor: Sichuan Deyue Technology Seed Industry Co., Ltd. Registration number: 2018510000104 |