CN103168119B - 用于产生、确认和使用单克隆抗体的方法和系统 - Google Patents

用于产生、确认和使用单克隆抗体的方法和系统 Download PDF

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Publication number
CN103168119B
CN103168119B CN201180039055.8A CN201180039055A CN103168119B CN 103168119 B CN103168119 B CN 103168119B CN 201180039055 A CN201180039055 A CN 201180039055A CN 103168119 B CN103168119 B CN 103168119B
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China
Prior art keywords
antibody
antibodies
library
protein
antigens
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English (en)
Chinese (zh)
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CN103168119A (zh
Inventor
杰夫·D·伯克
恒·朱
塞斯·布莱克肖
丹尼尔·J·艾兴格
伊格纳西奥·皮诺
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Cdi Lab Corp
Johns Hopkins University
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Cdi Lab Corp
Johns Hopkins University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180039055.8A 2010-06-16 2011-06-16 用于产生、确认和使用单克隆抗体的方法和系统 Active CN103168119B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35532910P 2010-06-16 2010-06-16
US61/355,329 2010-06-16
PCT/US2011/040789 WO2011159959A2 (en) 2010-06-16 2011-06-16 Methods and systems for generating, validating and using monoclonal antibodies

Publications (2)

Publication Number Publication Date
CN103168119A CN103168119A (zh) 2013-06-19
CN103168119B true CN103168119B (zh) 2016-05-11

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Country Link
US (3) US20130288910A1 (https=)
EP (1) EP2582862B1 (https=)
JP (2) JP2013533865A (https=)
CN (1) CN103168119B (https=)
CA (1) CA2802868A1 (https=)
WO (1) WO2011159959A2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012103260A2 (en) * 2011-01-25 2012-08-02 Cdi Laboratories High-throughput methods to produce, validate and characterize mmabs
US20140307931A1 (en) * 2013-04-15 2014-10-16 Massachusetts Institute Of Technology Fully automated system and method for image segmentation and quality control of protein microarrays
EP3011061B1 (en) * 2013-06-21 2021-12-29 The Johns Hopkins University Virion display array for profiling functions and interactions of human membrane proteins
WO2017177140A1 (en) * 2016-04-07 2017-10-12 Rowan University Methods and kits for detecting myo/nog cells in human adipose tissue and methods of use thereof
CN109470852A (zh) * 2018-12-07 2019-03-15 华南农业大学 一种母乳多克隆抗体免疫亲和柱及其制备方法
WO2025101487A1 (en) * 2023-11-06 2025-05-15 Bruker Cellular Analysis, Inc. Affinity assay for microfluidic devices

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1786298A (zh) * 2005-10-28 2006-06-14 上海博昂抗体生物科技有限公司 抗细胞或组织蛋白质单克隆抗体库的制备方法

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DE4134297A1 (de) * 1991-10-17 1993-04-22 Behringwerke Ag Monoclonale antikoerper gegen mycoplasma pneumoniae, diese produzierende hybridome, verfahren zu deren herstellung sowie deren verwendung
CA2319828A1 (en) * 1998-01-29 1999-08-05 Miller, Samuel High density arrays for proteome analysis and methods and compositions therefor
US6794132B2 (en) * 1999-10-02 2004-09-21 Biosite, Inc. Human antibodies
CA2506127C (en) * 2002-11-15 2013-07-09 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
WO2006033972A2 (en) * 2004-09-15 2006-03-30 Protometrix, Inc. Protein arrays and methods of use thereof
ATE553385T1 (de) * 2004-10-23 2012-04-15 Biosystems Technology Corp Plattformtechnologie zur expressionsprofilierung
MX2007006441A (es) * 2004-11-30 2007-08-14 Johnson & Johnson Prognosis de cancer de pulmon.
WO2006124644A2 (en) * 2005-05-12 2006-11-23 Board Of Regents, The University Of Texas System Protein and antibody profiling using small molecule microarrays
EP1921142A1 (en) * 2006-11-07 2008-05-14 Cytos Biotechnology AG Selection of human monoclonal antibodies by eukaryotic cell display
WO2009108170A2 (en) * 2007-11-16 2009-09-03 Invitrogen Corporation Compositions and methods for determining immune status
US20100034807A1 (en) * 2008-07-25 2010-02-11 Matthew Moyle Methods and compositions for discovery of target-specific antibodies using antibody repertoire array (ara)
JP2013506138A (ja) * 2009-09-25 2013-02-21 オリジーン・テクノロジーズ・インコーポレイテツド タンパク質アレイおよびその使用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1786298A (zh) * 2005-10-28 2006-06-14 上海博昂抗体生物科技有限公司 抗细胞或组织蛋白质单克隆抗体库的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Analyzing antibody specificity with whole proteome microarrays;Michaud 等;《Nature Biotechnology》;20031109;第21卷(第12期);第1509-1512页 *

Also Published As

Publication number Publication date
EP2582862A4 (en) 2014-01-15
JP2013533865A (ja) 2013-08-29
US20180030086A1 (en) 2018-02-01
EP2582862B1 (en) 2016-04-13
WO2011159959A2 (en) 2011-12-22
US20220363712A1 (en) 2022-11-17
WO2011159959A3 (en) 2013-03-28
US20130288910A1 (en) 2013-10-31
HK1182745A1 (zh) 2013-12-06
CN103168119A (zh) 2013-06-19
CA2802868A1 (en) 2011-12-22
EP2582862A2 (en) 2013-04-24
JP2016222725A (ja) 2016-12-28

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