CN103141449A - Method for improving organophosphorus pesticide resistance of silkworms - Google Patents
Method for improving organophosphorus pesticide resistance of silkworms Download PDFInfo
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- CN103141449A CN103141449A CN2013100601418A CN201310060141A CN103141449A CN 103141449 A CN103141449 A CN 103141449A CN 2013100601418 A CN2013100601418 A CN 2013100601418A CN 201310060141 A CN201310060141 A CN 201310060141A CN 103141449 A CN103141449 A CN 103141449A
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Abstract
The invention discloses a method for improving the organophosphorus pesticide resistance of silkworms, which comprises the following steps: first, preparing nanometer oxide into an additive solution with the concentration of 5.0+/-0.5 mg/L; second, spraying the additive solution on proper mature leaves according to the use dosage that 1.0+/-0.2 L of additive solution is added on the mulberry leaves of 100 kg; and finally, adding the silkworms after the mulberry leaves are dried. According to the utility model, based on the biological functions of nanometer oxides and physiological features of the silkworm, the additive containing a plurality of nanometer oxides are prepared for the silkworms, the raw materials are wide in source, and the cost is low. The method has the advantages of strong enabling performance, convenience in operation, and obvious effect, and therefore is suitable for being applied in silkworm areas in our country.
Description
Technical field
The present invention relates to a kind ofly improve silkworm to the method for organophosphorus pesticide resistance, be specifically related to a kind ofly improve silkworm to the method for organophosphorus pesticide resistance by the nano particle effect.
Background technology
Silkworm (
Bombyx mori) be important economic insects, raised more than 5700 by domestication, its performance of producing silk has obtained significantly raising, but its to the resistance of chemical pesticide a little less than.In recent years, being widely used on agricultural and forestry production due to chemical pesticide, the silkworm pesticide poisoning is of common occurrence, cause silk cocoon seriously in poor harvest, even No kernels or seeds are gathered, as in a year of scarcity for some silkworm raisers, greatly dampened silkworm raiser's enthusiasm for production, serious threat silkworm and mulberry prodution and development, pesticide poisoning causes that silkworm does not cocoon to have become affects the key factor that Silk Industry is produced.Organophosphorous pesticide is efficient owing to having, the feature of wide spectrum and low toxicity, is widely used on producing, because organophosphorous pesticide causes poisoning more than 8 one-tenth of silkworm poisoning that account for of silkworm.So in the urgent need to taking the necessary measures, improve silkworm to the resistance of organophosphorous pesticide in production, realize that China's silk develops in a healthy way.
On the other hand, nano-oxide has thermal and magnetic, light-sensitive characteristic and the surface stability that is better than conventional particle due to its unique small-size effect, skin effect, quantum size effect and macro quanta tunnel effect, thereby be applied to various aspects, as the coating in industry and pigment field, pottery in daily life, sun care preparations and field of food are used for air cleaning, waste water treatment and antibiotic except malicious field in environmental improvement.Antibacterial agent is with nano-size dispersion in nano anti-biotic material, have high-specific surface area and high reaction activity, the more traditional antibacterial agent of the antibacterial effect of anti-biotic material integral body is significantly increased, microbial growth and the breedings such as anti-bacteria, fungi more significantly, simultaneously the efficient catalytic performance of nano-oxide can impel again the gas degraded (Li Yiling etc. nano anti-biotic material application study progress. scientific and technical innovation Leader 2009 (3): 1).
Therefore it is necessary to the method for organophosphorus pesticide resistance that the combining nano properties of materials is developed the raising silkworm of a kind of low cost, instant effect.
Summary of the invention
The purpose of this invention is to provide and a kind ofly improve silkworm to the method for organophosphorus pesticide resistance, to promote the sound development of Silk Industry.
To achieve the above object of the invention, the technical solution used in the present invention is: a kind ofly improve silkworm to the method for organophosphorus pesticide resistance, comprise the following steps:
(1) preparation of additive solution: with nano-oxide be mixed with the solution that concentration is 4.5～5.5mg/L after water mixes; Wherein nano-oxide is the mixture of nano titanium oxide, nano silicon, nano zine oxide, nano aluminium oxide, nano-cerium oxide, nano-sized iron oxide;
(2) add the food silkworm: the solution of preparation in step (1) is added 0.8～1.2L solution dosage sprinkling proper mature (ripe) leaf according to the 100kg mulberry leaf, add the food silkworm and can improve silkworm to organophosphorus pesticide resistance after the proper mature (ripe) leaf dries.
In technique scheme step (1), nano titanium oxide (TiO
2), nano silicon (SiO
2), nano zine oxide (ZnO), nano aluminium oxide (Al
2O
3), nano-cerium oxide (CeO
2), nano-sized iron oxide (Fe
2O
3) mol ratio be 1.0: 0.8: 0.7: 0.8: 1.0: 0.6.
The present invention, has developed silkworm and has added agent with nanometer in conjunction with the physiological characteristic of silkworm according to the biological function of each oxide; The solution of preparation abundant mixing before use, add 1.0 ± 0.2L dosage according to the 100kg mulberry leaf and evenly spray the proper mature (ripe) leaf in step (1), and I haven't seen you for ages affect result of use for amount, and amount is high can the increase use cost; Add the food silkworm after mulberry leaf dry naturally, avoid eating lower moisture content too much, affect digestion and the absorption of feed, the same with usual usage amount to the mulberry amount; Nanometer additive can mix use with other silkworm medicines and additive, need not affect result of use.
The present invention adopts leaf dipping method to detect silkworm to the pesticide resistance of organophosphorous pesticide: detect test region and check plot silkworm to the half lethal concentration LC50 of organophosphorous pesticide.
In technique scheme, the computational methods of LC50 are by open (the Bing Li of Bing Li, Yanhong Wang, Haitao Liu, YaXiang Xu, Zhengguo Wei, YuHua Chen and Weide Shen. Resistance comparison of domesticated silkworm (
Bombyx moriL.) and wild silkworm (
Bombyx mandarinaM.) to phoxim insecticide. African Journal of Biotechnology 2,010 9 (12): 1771), those skilled in the art can reference.
Because technique scheme is used, the present invention has following advantages:
The present invention utilize nano-oxide as additive feeding silkworm to improve its organophosphorus pesticide resistance, effect is remarkable, can improve silkworm to organophosphorus pesticide resistance more than 10 times, and applicable all cultivated silkworm breed varieties and all length of time, has versatility;
2. the additive amount of using in method disclosed by the invention is few, wide material sources, cost are low; Need not to change production law, easy to operate, be easy to apply.
Embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment one: a kind ofly improve silkworm to the method for organophosphorus pesticide resistance
⑴ material and medicine:
Cultivated silkworm breed variety: Su Xiu * spring is rich, is bred and is preserved by this laboratory.
Nano-oxide: nano titanium oxide (TiO
2), nano silicon (SiO
2), nano zine oxide (ZnO), nano aluminium oxide (Al
2O
3), nano-cerium oxide (CeO
2), nano-sized iron oxide (Fe
2O
3) be Hangzhou ten thousand scape new material Co., Ltd products, get above-mentioned oxide and mix with water and be mixed with the additive that concentration is 5.0mg/L.
Organophosphorous pesticide is phoxim, Sigma company product.
⑵ the use of additive
Adopt abundant mixing additive before use, add 1.0L dosage according to the 100kg mulberry leaf and spray the proper mature (ripe) leaf in each length of time, mulberry tree breed adds the food silkworm for educating 711 after mulberry leaf dry, and is the same with usual usage amount to the mulberry amount.
⑶ pesticide resistance detects
Adopt leaf dipping method, the silkworm in the 3rd day 5 ages is carried out pesticide resistance detect: phoxim is mixed with different concentration gradients, and every district 30 boss silkworms are established three duplicate blocks.With reference to the disclosed method of Bing Li, the pesticide resistance of test region and check plot (not adding the food nano-oxide) silkworm is detected, result is as shown in table 1.
Table 1 virulence detection statistics table
Above result is carried out Chi-square Test, result of calculation shows the silkworm LC50(24h that adds the food nano-oxide) be 92.45mg/L, 95% confidence limit is 87.86 mg/L ~ 97.28mg, the check plot is LC50(24h) be 8.76mg/L, 95% confidence limit is 8.40 mg/L ~ 9.14mg, and resistance increases by 10.55 times.
Claims (4)
1. one kind is improved silkworm to the method for organophosphorus pesticide resistance, it is characterized in that, comprises the steps:
(1) preparation of additive solution: with nano-oxide be mixed with the solution that concentration is 4.5～5.5mg/L after water mixes; Wherein nano-oxide is the mixture of nano titanium oxide, nano silicon, nano zine oxide, nano aluminium oxide, nano-cerium oxide, nano-sized iron oxide;
(2) add the food silkworm: the solution of preparation in step (1) is added 0.8～1.2L solution dosage sprinkling proper mature (ripe) leaf according to the 100kg mulberry leaf, add the food silkworm and can improve silkworm to organophosphorus pesticide resistance after the proper mature (ripe) leaf dries.
2. the method for raising according to claim 1 silkworm to organophosphorus pesticide resistance, it is characterized in that: the mol ratio of nano titanium oxide, nano silicon, nano zine oxide, nano aluminium oxide, nano-cerium oxide, nano-sized iron oxide is 1.0: 0.8: 0.7: 0.8: 1.0: 0.6.
3. the method for raising silkworm according to claim 1 to organophosphorus pesticide resistance, is characterized in that: drying for naturally drying in described step (2).
4. the method for raising according to claim 1 silkworm to organophosphorus pesticide resistance is characterized in that: the same with usual usage amount to the mulberry amount when adding the food silkworm in described step (2).
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| CN201310060141.8A CN103141449B (en) | 2013-02-26 | 2013-02-26 | Method for improving organophosphorus pesticide resistance of silkworms |
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|---|---|---|---|
| CN201310060141.8A CN103141449B (en) | 2013-02-26 | 2013-02-26 | Method for improving organophosphorus pesticide resistance of silkworms |
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|---|---|
| CN103141449A true CN103141449A (en) | 2013-06-12 |
| CN103141449B CN103141449B (en) | 2014-07-16 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103564196A (en) * | 2013-11-15 | 2014-02-12 | 苏州大学 | Additive for improving bombyx mori yield |
| CN104839202A (en) * | 2015-05-05 | 2015-08-19 | 贺州学院 | Nanometer iron oxide composite triazophos type pesticide and preparation method thereof |
| CN104855342A (en) * | 2015-04-29 | 2015-08-26 | 东华大学 | Solution containing high-biocompatibility nanometer copper sulphide and preparation method of same |
| CN108308127A (en) * | 2018-05-17 | 2018-07-24 | 江苏科技大学 | A method of silkworm is improved to BmCPV virus resistances |
| CN113142384A (en) * | 2021-04-13 | 2021-07-23 | 苏州大学 | Method for improving pesticide resistance of silkworms to phoxim |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468937A (en) * | 2002-07-18 | 2004-01-21 | 青岛海洋大学 | Residual pesticide degrading agent |
| CN101822975A (en) * | 2009-03-06 | 2010-09-08 | 五邑大学 | Composite photocatalyst for degrading pesticide residues |
| CN101822974A (en) * | 2009-03-06 | 2010-09-08 | 五邑大学 | Method for preparing pesticide residue degradation agent |
| CN102107946A (en) * | 2009-12-29 | 2011-06-29 | 陈皓琳 | Technique for treating organophosphorus pesticide wastewater under photocatalysis |
| CN102499335A (en) * | 2011-11-15 | 2012-06-20 | 湖南农业大学 | Method for degrading organophosphorus pesticide in fruits and vegetables with nanometer degradation agent |
| CN103081733A (en) * | 2012-10-17 | 2013-05-08 | 王浅肃 | Method for eliminating pesticide residues |
-
2013
- 2013-02-26 CN CN201310060141.8A patent/CN103141449B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468937A (en) * | 2002-07-18 | 2004-01-21 | 青岛海洋大学 | Residual pesticide degrading agent |
| CN101822975A (en) * | 2009-03-06 | 2010-09-08 | 五邑大学 | Composite photocatalyst for degrading pesticide residues |
| CN101822974A (en) * | 2009-03-06 | 2010-09-08 | 五邑大学 | Method for preparing pesticide residue degradation agent |
| CN102107946A (en) * | 2009-12-29 | 2011-06-29 | 陈皓琳 | Technique for treating organophosphorus pesticide wastewater under photocatalysis |
| CN102499335A (en) * | 2011-11-15 | 2012-06-20 | 湖南农业大学 | Method for degrading organophosphorus pesticide in fruits and vegetables with nanometer degradation agent |
| CN103081733A (en) * | 2012-10-17 | 2013-05-08 | 王浅肃 | Method for eliminating pesticide residues |
Non-Patent Citations (1)
| Title |
|---|
| 李兵等: "家蚕和野桑蚕对辛硫磷农药抗性的研究", 《中国蚕学会第六届青年学术研讨会论文集（3）》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103564196A (en) * | 2013-11-15 | 2014-02-12 | 苏州大学 | Additive for improving bombyx mori yield |
| CN104855342A (en) * | 2015-04-29 | 2015-08-26 | 东华大学 | Solution containing high-biocompatibility nanometer copper sulphide and preparation method of same |
| CN104855342B (en) * | 2015-04-29 | 2017-03-22 | 东华大学 | Solution containing high-biocompatibility nanometer copper sulphide and preparation method of same |
| CN104839202A (en) * | 2015-05-05 | 2015-08-19 | 贺州学院 | Nanometer iron oxide composite triazophos type pesticide and preparation method thereof |
| CN108308127A (en) * | 2018-05-17 | 2018-07-24 | 江苏科技大学 | A method of silkworm is improved to BmCPV virus resistances |
| CN113142384A (en) * | 2021-04-13 | 2021-07-23 | 苏州大学 | Method for improving pesticide resistance of silkworms to phoxim |
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| Publication number | Publication date |
|---|---|
| CN103141449B (en) | 2014-07-16 |
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