CN103126970A - Composition for preventing hair loss or improving hair growth - Google Patents
Composition for preventing hair loss or improving hair growth Download PDFInfo
- Publication number
- CN103126970A CN103126970A CN2012104954931A CN201210495493A CN103126970A CN 103126970 A CN103126970 A CN 103126970A CN 2012104954931 A CN2012104954931 A CN 2012104954931A CN 201210495493 A CN201210495493 A CN 201210495493A CN 103126970 A CN103126970 A CN 103126970A
- Authority
- CN
- China
- Prior art keywords
- radix
- compositions
- ginseng
- weight
- bran koji
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention relates to a composition which is used for hair and scalp improvement and comprises more than one selected from the group consisting of scrophularia ningpoensis, ginseng, salviae miltiorrhizae, astragalus membranaceus, codonopsis pilosulae, radix sophorae flavescentis, and adenophora stricta. The scalp and hair cosmetic composition containing more than one mouldy bran fermentation extract selected from the group consisting of extracts of scrophularia ningpoensis, ginseng, salviae miltiorrhizae, astragalus membranaceus, codonopsis pilosulae, radix sophorae flavescentis, and adenophora stricta displays effects of papilla cell proliferation, blood circulation promotion, and antioxidation, thereby being capable of providing effects of preventing hair loss or improving hair growth.
Description
Technical field
More than one the hair of bran koji fermented thing and the scalp that the present invention relates to contain in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae) improves and uses compositions.
Background technology
Alopecia is divided into telogen effluvium and growing period alopecia according to growth cycle, the alopecia that telogen effluvium has male pattern baldness, women's puerperal alopecia, endocrinopathy that androgen causes to cause; (Kuag, 2001) such as alopecias that growing period alopecia has circular alopecia, cicatricial alopecia and tinea capitis to cause.In addition, known local infection (Muller et al., 1980), dyshormonia (Bergfeld, 1978), genetic factor (Selman, wilzet al., 1974) and oneself's immunity (Friedman, 1981) etc. also can cause the generation of alopecia.
At present, representational Anti-hair loss agent has finasteride that U.S. FDA admits and Minoxidil etc.Although this medicine shows clear and definite effect clinically, have the side effect such as sexual dysfunction and skin irritation, therefore forbid or limit the women and use.Recently, people actively develop the new hair growth promoter that can supply these medicine shortcomings and the natural goods raw material that not used by the sex restriction.
Disclose in Korean registered patent 10-0581316 contain fill second (
), the Anti-hair loss of the mixed extract of Nardostachys chinensis Batal., Flos Chrysanthemi, Semen Persicae, Fructus Corni, Semen Cuscutae, Fructus Schisandrae Chinensis, the Rhizoma Atractylodis Macrocephalae, Folium Pini and several Korea Spro's medical materials of Radix Angelicae Sinensis and promote the compositions of hair growth promoting; The Korean registered patent discloses the ethanol extraction that contains angelica root and Radix Arctii No. 10-0609210, by the testosterone in angelica root element composition-Anti-hair loss shampoo composite that the improving blood flow effect of the inulin composition of alpha-reductase inhibition and Radix Arctii has the Anti-hair loss effect.But much all can't clearly define the mechanism of action of the Anti-hair loss effect that shows clinically in above-mentioned example, yet there are no propagation, blood circulation facilitation effect and scalp antioxidant effect that the report bran koji fermented extract that utilizes 7 kinds of medicine conopsea extractions of the present invention has the papilla cell.
Summary of the invention
Goal of the invention
The present inventor provides the compositions of the natural goods raw material with Anti-hair loss effect and effort safely in order to develop conveniently have no side effect from the daily life aspect the same with shampoo of a kind of utilization, found that will be selected from more than one in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae) carries out bran koji fermented, can give propagation, blood circulation promotion, antioxidant effect to the papilla showed cell, thereby complete the present invention.
Technical scheme
In order to achieve the above object, the invention provides and contain more than one the compositions that is used for hair and scalp condition improvement of bran koji fermented thing that is selected from Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae).
Below describe the present invention in detail.
The Radix Scrophulariae (Scrophularia buergeriana) that uses according to the compositions of the embodiment of the present invention is the herbaceos perennial of Scrophulariaceae, is called again Chong Tai, Xuan Tai, terrible Tibetan, pursues horse.In South Korea, its root is called Radix Scrophulariae, is used for pharyngolaryngitis, furuncle, lymphadenitis as antipyretic.
The Radix Ginseng that uses according to the compositions of the embodiment of the present invention be Araliaceae (
) root of Radix Ginseng (Panax ginseng C.A.Meyer), refer to the root that radicula and phellem layer are removed.Especially, Radix Ginseng divided into Radix Ginseng (stranger's ginseng), Radix Ginseng Rubra (Radix Ginseng of steaming) and tail ginseng (thin following) and put down in writing in Korea S, but also being divided into long brain ginseng and SHANSHEN at wild-growing ginseng among the people.Refer to root and the rhizome of Radix Ginseng in China, divide into Park Ginseng (supporting ginseng), Radix Ginseng Rubra, SHANSHEN (wild-growing ginseng).Radix Ginseng is because the shape of its root with humanoid similar and gain the name, because have magical effect, also is called as SHENCAO, belongs to senior medicinal herbs and the people that is otherwise known as rank, also is called as Coprinus atramentarius (Bull.) Fr. (Coprinus sterquilinus Fr.) because detesting sun happiness the moon.This medical instrument has special abnormal smells from the patient, distinguishes the flavor of little sweet, and the character tepor.The Radix Ginseng invigorating primordial QI for weak, asthenia, fatigue, inappetence, vomiting, diarrhoea, helps the pulmonary function lung benefiting to promote the production of body fluid, and can calm the nerves, and improve renal function.The pharmacological action that is in the news has corticocerebral excitement and inhibition, balance, resisting fatigue, aging resistance, immunostimulant, heart contraction, gonad to promote, hyperglycemia suppresses, protein synthesis promotes, dynamic equilibrium is kept, anticancer, Detoxication etc.Outward appearance is elongated cylindrical or spindle, has 2-5 root lateral root, and skin is yellowish-brown, has horizontal wrinkle and root trace.The root head has stem trace reed bowl.
The Radix Salviae Miltiorrhizae that uses according to the compositions of the embodiment of the present invention is the medical material (Korea S, China) of the root manufacturing of Radix Salviae Miltiorrhizae (Salvia miltiorrhiza Bunge), does not use in Japan.Five seed ginsengs-Radix Ginseng, Radix Sophorae Flavescentis, Radix Salviae Miltiorrhizae, Radix Scrophulariae, Radix Adenophorae (Radix Glehniae)-be the five colors adhere to the five internal organs separately, and Radix Salviae Miltiorrhizae enters heart channel, and Radix Salviae Miltiorrhizae is otherwise known as.In addition, Radix Salviae Miltiorrhizae also because the treatment syndrome caused by wind pathogen is eliminated weakness of the lower extremities, can make the people catch up with a dry goods and gain the name to Herba Setariae Plicatae.This medical instrument has slightly special fragrance, and the property of medicine is pained, is slightly cold.Help blood circulation, except congestion, alleviate extremities joint pain.Treatment women's physiology has some setbacks, dysmenorrhea, puerperal abdonimal pain, congestion trusted subordinate pain, traumatic injury etc.Also treat the spirit dusk that high heat causes dim, say ravings, agitation, hyposomnia, skin dermexanthesis, cardiopalmus etc.The pharmacological action that is in the news has coronary artery expansion, cholesterol reducing, blood pressure lowering, liver function activation, calm antiinflammatory, anticancer, antibacterial action.
The Radix Astragali that uses according to the compositions of the embodiment of the present invention is the herbaceos perennial of pulse family, is called again Huang Qi.The two bucket scented teas of Korai period
In be called several plates fiber crops, Korea is called sweet plate fiber crops the initial stage, becomes Radix Sophorae Flavescentis without concealing anything after entering for the 1600's
Begin to be called as the Radix Astragali to the 1700's.This is because and the name that be endowed similar to the Radix Sophorae Flavescentis taste.Formal name used at school is Astragalusmembranaceus BUNGE.Give birth in the mountain region, root is as medical material, and effective ingredient is folic acid (folic acid), choline (choline) etc.Excitation and diuresis to the central nervous system in zoopery are remarkable, can suppress the generation of nephritis when giving a large amount of powder of white mouse, postpone the generation of albuminuria and hyperlipemia, also have the effect of blood pressure lowering.This medicine property of medicine is gentle little sweet.To weak, voice are overcast, a little less than pulse, the patient such as hyperhidrosis has significant effect, the Radix Ginsengs that replace use more from ancient times.Also be widely used in addition uterine prolapse, gastroptosis, proctoptosis, metrorrhagia, physical strength reinforcing, the tensity of raising whole-body muscle.Can not use when serious but generate heat.Among the people pass on from one to another and chicken together Baoshang take and will treat sweating due to debility, physical strength reinforcing and being widely used.Representational prescription has multiplex people's in feeling weak, lose appetite BUZHONG YIQI TANG.
The climing ginseng of using according to the compositions of the embodiment of the present invention is dicotyledon, and the root of the tendril property perennial herb Radix Codonopsis of Campanulales campanulaceae (Codonopsis pilosula Nannfeldt) is called again Radix Codonopsis or ginseng, as expectorant or edible.Climing ginseng replaces Radix Ginseng to be used for Radix Ginseng is had the people of side effect, is to have the famous medicine of identical effect with Radix Ginseng.This medical instrument has special fragrance, distinguishes the flavor of little sweet, and property is gentle and not inclined to one side.Radix Codonopsis to the insufficiency of the spleen and physical fatigue that produces, digest and assimilate bad, cough, thirsty, the deficiency of vital energy, blood deficiency and produce effect.The pharmacological action that is in the news has the hyperfunction effect of strong effect, resisting fatigue and immunologic function, erythrocyte number increasing action, peptic ulcer, blood pressure lowering and heart, brain, lower limb, SBF increasing action.
The Radix Sophorae Flavescentis that uses according to the compositions of the embodiment of the present invention is the root after Radix Sophorae Flavescentis (Sophora flavescens Aiton) is removed perithelium.The name of Radix Sophorae Flavescentis is because bitter in the mouth uses bitter word, and its effect and Radix Ginseng are similar and use and join word.The plant name that has thief's crutch due to the shape of root.This medical instrument has special abnormal smells from the patient, has residual, and bitter in the mouth is cold in nature.Be used for dysentery that damp-heat in lower-JIAO causes, leukorrhagia, pudendal pruritus, skin pruritus etc., be used for also that urine that bladder heat causes has some setbacks and when pain disease is arranged.The pharmacological effect that is in the news has leukopenia treatment and radiation resistance, coronary artery blood flow increase, cardiac muscle reinforcement, hypoglycemic activity, antitumor, antibiotic, immunologic function inhibitory action.
The Radix Adenophorae (Radix Glehniae) that uses according to the compositions of the embodiment of the present invention is the medical material (Korea S) that the root of liliaceous Adenophora triphylla var.japonica Hara (Adenophora triphylla var.japonica Hara) or its congener is done, at Japan fine leaf adenophora (Adenophora triphylla(Thunb.) A.DC.), at middle national expenditures Radix Glehniae (Adenophora stricta Miq) and adenophora tetraphylla (Adenophora tetraphylla(THUNB.) FISCH) make.The same Radix Ginseng of described Radix Adenophorae (Radix Glehniae), Radix Scrophulariae, Radix Salviae Miltiorrhizae, Radix Sophorae Flavescentis together are called as five ginsengs, although this is that all different effect is similar because of form.The name of Radix Adenophorae (Radix Glehniae) is because white and be grown in sand ground and the name that rises.Be sheep mother-in-law milk because root is rich in white juice by valgar person.This medical instrument has special fragrance, has chewed mucus, little sweet, cold nature of distinguishing the flavor of.Radix Adenophorae (Radix Glehniae) promoting the production of body fluid for nourishing the lung, fall heat, eliminate the phlegm, be used for dry laryngopharynx, dry cough, expectorant, cough, asthma etc.Be used for constipation symptom that xerostomia, larynx that deficiency of stomach-Yin causes do, be hard and dry etc.The pharmacological action that is in the news has phlegm-dispelling functions, antibacterial action, haemolysis, cardiotonic etc.
In the present invention, above-mentioned 7 kinds of medicines ginseng is the medical material with blood (Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali), nutrition (Radix Scrophulariae, climing ginseng), protection (Radix Sophorae Flavescentis, Radix Adenophorae (Radix Glehniae)) effect that filters out from Dong-eui-bo-gam and Korea S's book on Chinese herbal medicine illustrated handbook.
The compositions of using in the present invention is made by the following method.
At first, thus more than one in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae) ferment with Fuqu makes compositions according to the embodiment of the present invention.
Also have, after obtaining more than one the extract in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae), carry out the said extracted thing bran koji fermented and make compositions according to the embodiment of the present invention.
Fuqu as used herein is representational natural maturation leaven, as far back as invention in period in China spring autumn, and is that three states are before the epoch in Korea S.Fuqu is that the Fuqu of enzyme with wine brewing is mould and yeast is bred in the frumentum Fructus Hordei Vulgaris, the mould kind more than 50 of having an appointment of known Fuqu.Wherein, representational Aspergullus oryzae(Aspergillus flavus arranged) and Aspergillus niger(aspergillus niger), they have the enzyme with the brute force of rice starch saccharifying, therefore can carry out to be disaccharides and monosaccharide, finally to be converted into the enzyme conversion reaction of ethanol amylolysis.Except wine fermentation, these enzymes can be converted into the glycocide of isopropyl alcohol and polyphenol the non-glycoside body that increases the physiologically active form.The result that the fact that hands the operator of factory's work of making salty sauce or wine brewing is bleached is studied has been found the composition kojic acid that Fuqu is mould.Yeast not only is used for wine brewing, and also containing with biosis has the plurality of enzymes of profound relation and vitamin B complex, protein, inanimate matter etc.
Above-mentioned manufacture method is further described as follows, for example, more than one in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis, Radix Adenophorae (Radix Glehniae) are got up with Gunny Bale (Hessian Cloth Bag), imbed in the cylinder that is mixed with Fuqu and rice.After 35 ℃ of bottom fermentation 2-3 days, carry out the heat extraction and obtain compositions, but be not limited to this.
Another manufacture method can be that for example, more than one in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis, Radix Adenophorae (Radix Glehniae) are imbedded this in cylinder that is mixed with Fuqu and rice after obtaining extract by common natural goods preparation method.After 35 ℃ of bottom fermentation 2-3 days, carry out the heat extraction and obtain compositions, but be not limited to this.
The method that further describe to obtain the medicine conopsea extraction is as follows, for example, more than one in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis, Radix Adenophorae (Radix Glehniae) is washed, after drying, this is carried out solvent extraction, filters after extraction and obtains.Described solvent can be selected from more than one in the group of compositions of mixtures of water, organic solvent and water and organic solvent.At this, water comprises distilled water or purified water, and described organic solvent is the lower alcohol of C1-C5, for example comprises more than one in the group that is selected from alcohol, acetone, ether, ethyl acetate, ether, methyl ethyl ketone and chloroform composition, but is not limited to this.
The bran koji fermented phase of the medicine ginseng of more than one in the present invention in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae) is preferably the 0.01-10 % by weight for the gross weight of compositions, in the situation that there is no scalp irritation agent allergy in above-mentioned scope, have excellent hair and scalp condition improvement effect.
Beneficial effect
More than one scalp and the cosmetic hair composition of bran koji fermented extract that contains in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae) extract of the present invention, demonstrate papilla cell proliferation, blood circulation promotion and antioxidant effect, thereby anti-loss phenomenon, trichogenous effect can be provided.
Description of drawings
Fig. 1 means that compositions according to the present invention is to the collection of illustrative plates of hair follicle papilla cell proliferation facilitation effect.
Fig. 2 means the active collection of illustrative plates that increases effect of calcium channel related according to compositions of the present invention pair and blood circulation facilitation effect.
Fig. 3 means the collection of illustrative plates according to the antioxidant effect of compositions of the present invention.
The specific embodiment
Reach by the following examples experimental example the present invention is specifically described, but protection scope of the present invention and can't help these restrictions.
The manufacturing of Production Example 1 medicine conopsea extraction
The extract of Radix Scrophulariae of the present invention, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and 7 kinds of medicine ginsengs of Radix Adenophorae (Radix Glehniae) is made by the following method.Above-mentioned medicine ginseng respectively with after the flowing water washing, is filtered with 250 orders (mesh) filter, and drying is 24 hours under 50 ℃.Add 70% ethanol of 1L in each medicine ginseng 50g of drying, at 7 kinds of each 50g of dried drugs ginseng mixture 350g() in 70% ethanol of interpolation 10L, carry out the reflux, extract, of 48 hours under the temperature conditions of 4 ℃.Filter with 250 order filters afterwards, carry out sedimentation, again filter with 0.5 μ m filter, obtain each medicine conopsea extraction and mixed extract.
The manufacturing of Production Example 2 bran koji fermented extracts
The compositions of using in the present invention contains the bran koji fermented extract of described medicine ginseng or their mixed extract as effective ingredient, makes by the following method.After 7 kinds of medicine ginsengs (Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis, Radix Adenophorae (Radix Glehniae)) are cleaned with flowing water, filter with 250 order filters, drying is 24 hours under 50 ℃.Wrap up respectively the medicine of above-mentioned drying with burlap and join each 50g and 7 kinds of each 50g of dried drug ginseng mixture 350g(), imbed in the cylinder of Fuqu and rice 1:1 mixing.Burlap is taken out after 3 days at 35 ℃ of bottom fermentations.Add the purified water of 20 times of fermentation medicine ginseng weight, the heat of carrying out under 80 ℃ 24 hours is extracted, and obtains bran koji fermented extract.
The manufacturing of reference example 1 comparative example 1-9 and embodiment 1-8
According to the composition of following table 1, use the extract of Production Example 1 to make comparative example 1-9, use the bran koji fermented extract of Production Example 2 to make embodiment 1-8.
Table 1
Experimental example 1 hair follicle papilla cell proliferation effect test
Consist of the keratin of hair by Keratinocyte (keratinocyte) generation of Rhizoma Imperatae section, this Keratinocyte is from the papilla cell differentiation.In order to estimate the papilla cytoactive according to compositions of the present invention, used in the present invention DP6(mice Immortalized dermal papilla cell (rat immortalized dermal papilla cell)) cell strain (Wendy Filsell, Journal of Cell Science 107,1761-1772 (1994)).This papilla cell strain is the cell strain that utilizes microdissection (microdissection) method separation and Culture from the beard Rhizoma Imperatae of male PVG mice, is containing 10% hyclone (Fetal bovine serum) DMEM (Dulbecco's modified Eagle's medium) (DMEM (FBS); Gibco BRL, Gaithersburg, MD, USA) in, keeping 5%CO
2, cultivated 24 hours in the calorstat (incubator) of 37 ℃.
With the comparative example 1-9 of table 1 composition and the DP6 of the above-mentioned cultivation of embodiment 1-8 processing, be placed in 96 orifice plates (96-well plate), cultivated 24 hours at the calorstats of 37 ℃.Comparative example 1 is the non-processed group of not using extract-treated.After drug treating 24 hours, use WST-1 test kit (WST-1kit) (Roche) to measure ability of cell proliferation, and shown in Figure 1.
Can find out from the result of Fig. 1, compare the comparative example 2-9 of medicine conopsea extraction own, remarkable with the papilla cell proliferation effect of the embodiment 1-8 of bran koji fermented extract-treated; With the embodiment 8 that the mixed extract of a kind of extract same concentrations is processed, compare that only the embodiment 1-7 effect with a kind of extract is remarkable, have better effect when therefore confirming the mixing use.
The active effect assessment that increases of experimental example 2 calcium channels
Alopeciaing therapeutic agent Minoxidil is known as potential mito-KATP channel opener (calcium channel opener), is the representative drugs of using in the treatment of androgen alopecia.In order to estimate the mechanism of this Minoxidil, process with the tolbutamide (Tolbutamide, SIGMA AlDRICH, T0891) that stops up the fibroblastic KATP passage that consists of scalp corium and suppress cell proliferation, then open the laboratory method that calcium channel recovers cell proliferation.
In order to estimate compositions of the present invention as the function of KATP channel opener, used fibroblast strain NIH3T3(mouse embryo fibroblast strain (Mouse embryonic fibroblast cell line) in the present invention) cell strain.This cell strain be separate from NIH Switzerland's mouse embryo (NIH Swiss mouse embryo) carry out the cell strain of nature infinite multiplication with 3T3 agreement (3T3 protocol).Described cell strain is at the DMEM (DMEM that contains 10% hyclone (FBS); Gibco BRL, Gaithersburg, MD, USA) in, keeping 5%CO
2, cultivated 24 hours in the calorstat of 37 ℃.NIH3T3 is placed in 96 orifice plates, cultivate after 24 hours in the calorstat of 37 ℃, process with the 2.5mM tolbutamide, composition according to table 2 and table 3 after 10 minutes is processed with the medicine conopsea extraction of positive controls Minoxidil 10 μ M and Production Example 1 and the bran koji fermented extract of Production Example 2 respectively, the process drug treating is after 48 hours, use WST-1 test kit (Roche) to measure the cell proliferation function, and with result shown in table 2.
Table 2
Table 3
Can confirm from the result of Fig. 2, when processing with embodiment 9-16, fibroblastic propagation is recovered to the level of the comparative example 12 of processing with Minoxidil, than the comparative example 13-20 that makes medication conopsea extraction itself, active to increase effect more excellent with the calcium channel of the embodiment 9-16 of bran koji fermented extract-treated, with the embodiment 16 that processes with the mixed extract of a kind of extract same concentrations, only compare the more remarkable effect with the embodiment 9-15 of a kind of extract, confirm thus to mix and have better effect when using.
The manufacturing of reference example 2 comparative example 21-31 and embodiment 17-24
According to the composition of following table 4 and 5, use the medicine conopsea extraction of above-mentioned Production Example 1 to make comparative example 21-31; Use the bran koji fermented extract of above-mentioned Production Example 2 to make embodiment 17-24.
Table 4
Table 5
Experimental example 3 antioxidant effect evaluations
In order to estimate antioxidant effect of the present invention, used the evaluation methodology of DPPH antioxidation.Esoteric oxidation reaction is mainly to be caused by free radical, and this effect can cause cell senescence and cancer.Thereby, free radical in body generates and suppresses finally can associate with antioxidation, aging resistance in body, this antioxidant effect is difficult is that object is tested with the free radical in body, therefore generally use DPPH(2,2-biphenyl-1-nitro hydrazyl (2,2-diphenyl-1-picrylhydrazyl)) free radical.The DPPH free radical is darkviolet, contacts with the antioxygenic effect material to become buff, measures absorbance at the 517nm wavelength and estimates the antioxidation energy.The material yellow that oxidation resistance is higher is more shallow, absorbance is lower.
Add the DPPH solution of 0.1mM in this experiment in 96 orifice plates after, with the comparative example 21-31 of described reference example 2 and embodiment 17-24 reaction 30 minutes in 37 ℃ of incubators respectively, measure absorbance at 517nm, and result is shown in Figure 3.Comparative example 21 is non-processor groups, and comparative example 22-23 uses respectively vitamin C (SIGMA, (+)-Sodium L-ascorbate) 50 μ g/ml, 100 μ g/ml to process as positive controls.
As can be seen from Figure 3, compare the matched group antioxidant effect with the embodiment 17-24 of bran koji fermented extract-treated of the present invention remarkable, compare the comparative example 24-31 that makes medication conopsea extraction itself, remarkable with the antioxidant effect of the embodiment 17-24 of bran koji fermented extract-treated.In addition, use the embodiment 24 that processes with the mixed extract of a kind of extract same concentrations, compare that only the embodiment 17-23 effect with a kind of extract is remarkable, have better effect in the time of can confirming thus on antioxidant effect the mixing use.
Claims (10)
1. one kind is used for Anti-hair loss or trichogenous compositions, it is characterized in that, said composition contains more than one bran koji fermented thing in Radix Scrophulariae, Radix Ginseng, Radix Salviae Miltiorrhizae, the Radix Astragali, climing ginseng, Radix Sophorae Flavescentis and Radix Adenophorae (Radix Glehniae) as effective ingredient.
2. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of the described Radix Scrophulariae of 0.01-10 % by weight.
3. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of the described Radix Ginseng of 0.01-10 % by weight.
4. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of the described Radix Salviae Miltiorrhizae of 0.01-10 % by weight.
5. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of the described Radix Astragali of 0.01-10 % by weight.
6. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of the described climing ginseng of 0.01-10 % by weight.
7. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of the described Radix Sophorae Flavescentis of 0.01-10 % by weight.
8. compositions according to claim 1, wherein, with respect to the gross weight of described compositions, described compositions contains the bran koji fermented thing of described Radix Adenophorae (Radix Glehniae) of 0.01-10 % by weight.
9. compositions according to claim 1, wherein, described compositions blood circulation promoting.
10. compositions according to claim 1, wherein, described compositions is for being used for scalp antioxidative compositions.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020110125710A KR101903745B1 (en) | 2011-11-29 | 2011-11-29 | Composition for improving hair growth or preventing hair loss |
| KR10-2011-0125710 | 2011-11-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103126970A true CN103126970A (en) | 2013-06-05 |
| CN103126970B CN103126970B (en) | 2017-07-18 |
Family
ID=48487853
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210495493.1A Active CN103126970B (en) | 2011-11-29 | 2012-11-28 | For Anti-hair loss or trichogenous composition |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP6139866B2 (en) |
| KR (1) | KR101903745B1 (en) |
| CN (1) | CN103126970B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105392492A (en) * | 2013-05-16 | 2016-03-09 | 济州大学校产学协力团 | Composition for preventing hair loss or promoting hair growth by containing bran extract as active ingredient |
| CN109908273A (en) * | 2019-04-28 | 2019-06-21 | 江苏医药职业学院 | It is a kind of for preventing or the pharmaceutical compositions of hair growth |
| CN115252696A (en) * | 2021-04-29 | 2022-11-01 | 新生活化妆品科技(上海)有限公司 | Composition capable of promoting collagen and angiogenesis and application |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102462458B1 (en) * | 2019-10-31 | 2022-11-02 | 주식회사 큐롬바이오사이언스 | Composition for preventing or treating of benign prostatic hyperplasia or alopecia comprising extracts of Salvia miltiorrhiza Bunge as an effective ingredient |
| KR102384238B1 (en) * | 2020-11-13 | 2022-04-07 | 대구한의대학교산학협력단 | Composition for preventing hair loss or promoting hair growth containing essential oil mixture of Schisandra fruit, cornus fruit, and Leonurus japonicus Houtt. |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05163121A (en) * | 1991-12-12 | 1993-06-29 | Soken:Kk | Hair restoring agent |
| JP2002255823A (en) * | 2001-02-27 | 2002-09-11 | Daicho Kikaku:Kk | Nutrient preparation, gastrointestinal preparation |
| KR20020084718A (en) * | 2001-05-02 | 2002-11-11 | 박의석 | Making Method Of Hair Tonic for Growimg Hair, Hair Removing Protection and Cleaning Head Skin |
| CN1765353A (en) * | 2004-10-28 | 2006-05-03 | 张震 | Anti-shed hair growth liquid prepared by apple, barley extract and Chinese medicine |
| CN101019816A (en) * | 2006-02-16 | 2007-08-22 | 茹国军 | Prepn process of hair blackening shampoo |
| WO2010051918A2 (en) * | 2008-11-04 | 2010-05-14 | Pharmasol Gmbh | Compositions containing lipid micro- or nanoparticles for the enhancement of the dermal action of solid particles |
| KR20120010699A (en) * | 2010-07-27 | 2012-02-06 | 닥터후 주식회사 | Composition for preventing hair loss or promoting hair growth comprising an extract of fermented ginseng |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0611686B2 (en) * | 1985-05-17 | 1994-02-16 | ポーラ化成工業株式会社 | Cosmetics |
| JP2676154B2 (en) * | 1988-08-11 | 1997-11-12 | 株式会社資生堂 | Hair restoration |
| JPH03123720A (en) * | 1989-10-06 | 1991-05-27 | Kanebo Ltd | Hair tonic cosmetic |
| JP2952627B2 (en) * | 1991-11-29 | 1999-09-27 | 株式会社タニサケ | Hair restoration liquid and method for producing the same |
| JP3283642B2 (en) * | 1993-06-16 | 2002-05-20 | 株式会社創研 | Hair restorer from rice |
| JPH083021A (en) * | 1994-06-16 | 1996-01-09 | Yoshito Hasegawa | Hair growth stimulant |
| JPH11158042A (en) * | 1997-12-03 | 1999-06-15 | Pola Chem Ind Inc | Trichogen |
| DE60137978D1 (en) * | 2001-09-28 | 2009-04-23 | Lg Household & Health Care Ltd | COMPOSITION FOR PROMOTING HAIR GROWTH |
| JP4075495B2 (en) | 2002-04-17 | 2008-04-16 | 三菱化学株式会社 | Polyurethane modified polypropylene resin solution |
| JP4693623B2 (en) * | 2005-03-07 | 2011-06-01 | 共栄化学工業株式会社 | Cosmetics |
| JP2007008965A (en) * | 2006-10-23 | 2007-01-18 | Pola Chem Ind Inc | Hair growing agent |
-
2011
- 2011-11-29 KR KR1020110125710A patent/KR101903745B1/en active IP Right Grant
-
2012
- 2012-11-27 JP JP2012258834A patent/JP6139866B2/en active Active
- 2012-11-28 CN CN201210495493.1A patent/CN103126970B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05163121A (en) * | 1991-12-12 | 1993-06-29 | Soken:Kk | Hair restoring agent |
| JP2002255823A (en) * | 2001-02-27 | 2002-09-11 | Daicho Kikaku:Kk | Nutrient preparation, gastrointestinal preparation |
| KR20020084718A (en) * | 2001-05-02 | 2002-11-11 | 박의석 | Making Method Of Hair Tonic for Growimg Hair, Hair Removing Protection and Cleaning Head Skin |
| CN1765353A (en) * | 2004-10-28 | 2006-05-03 | 张震 | Anti-shed hair growth liquid prepared by apple, barley extract and Chinese medicine |
| CN101019816A (en) * | 2006-02-16 | 2007-08-22 | 茹国军 | Prepn process of hair blackening shampoo |
| WO2010051918A2 (en) * | 2008-11-04 | 2010-05-14 | Pharmasol Gmbh | Compositions containing lipid micro- or nanoparticles for the enhancement of the dermal action of solid particles |
| KR20120010699A (en) * | 2010-07-27 | 2012-02-06 | 닥터후 주식회사 | Composition for preventing hair loss or promoting hair growth comprising an extract of fermented ginseng |
Non-Patent Citations (2)
| Title |
|---|
| C.X.PENG ET.AL: "Production of gastrodin through biotransformation of p-hydroxybenzyl alcohol using hairy root cultures of Datura tatula L.", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 * |
| 李东光 主编: "《日用化学品配方与制备手册》", 28 February 2010, 中国纺织出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105392492A (en) * | 2013-05-16 | 2016-03-09 | 济州大学校产学协力团 | Composition for preventing hair loss or promoting hair growth by containing bran extract as active ingredient |
| CN105392492B (en) * | 2013-05-16 | 2020-04-21 | 济州大学校产学协力团 | Composition for preventing hair loss or promoting hair growth containing wheat bran extract as active ingredient |
| CN109908273A (en) * | 2019-04-28 | 2019-06-21 | 江苏医药职业学院 | It is a kind of for preventing or the pharmaceutical compositions of hair growth |
| CN115252696A (en) * | 2021-04-29 | 2022-11-01 | 新生活化妆品科技(上海)有限公司 | Composition capable of promoting collagen and angiogenesis and application |
| CN115252696B (en) * | 2021-04-29 | 2023-10-03 | 上海如妍化妆品有限公司 | Composition capable of promoting collagen and angiogenesis and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6139866B2 (en) | 2017-05-31 |
| KR101903745B1 (en) | 2018-10-05 |
| JP2013112684A (en) | 2013-06-10 |
| CN103126970B (en) | 2017-07-18 |
| KR20130059640A (en) | 2013-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102533052B1 (en) | Korean traditional herbs shampoo composition | |
| CN101082017A (en) | Green grape health care wine | |
| CN103126970B (en) | For Anti-hair loss or trichogenous composition | |
| KR20180110939A (en) | Composition for preventing hair loss and promoting hair growth comprising plant extract | |
| CN102424625A (en) | Edible and medical fungi blackening agent, its preparation method and application | |
| CN101693862B (en) | Kudzu-vine root and codyceps healthcare wine and brewing method thereof | |
| CN103859521B (en) | Natural drinks of a kind of Morinda and preparation method thereof | |
| CN103173323A (en) | Preparation method of healthcare wine | |
| CN102465081A (en) | Beautifying health-care wine | |
| CN106106934A (en) | A kind of health protecting tea and preparation method thereof | |
| CN101530567A (en) | Traditional Chinese medicine composition for nourishing blood, tonifying kidney, growing hair and blacking hair and preparation method thereof | |
| CN102266498A (en) | Hair-blackening medicinal liquor and preparation process thereof | |
| CN101810328A (en) | Polygonatum fine dried noodles and manufacture method thereof | |
| CN104323953A (en) | Natural moisturizing and hydrating cream containing grifola frondosa extract | |
| CN101700355A (en) | Traditional Chinese medicine preparation for treating seborrheic alopecia | |
| CN101167897B (en) | Whitening and speckle-removing traditional Chinese medicinal beverage and preparation method thereof | |
| CN100400636C (en) | Notoginseng leaf beer brewing method | |
| CN105586224A (en) | Xiaoyao black soybean wine, honey wine black soybeans and preparation methods thereof | |
| CN107334937A (en) | A kind of preparation method for the health products for preventing and treating depression | |
| CN104222373A (en) | Rhizoma polygonati health tea and preparation method thereof | |
| CN103705728A (en) | Hair growing liquid and preparation method thereof | |
| CN103989846A (en) | Medicinal liquor for reducing blood pressure | |
| CN108686185A (en) | A kind of skin beautifying soup and its preparation method and application | |
| CN107951739A (en) | A kind of small peptide combinations of chicken embryo with white-skinned face function | |
| CN106344796A (en) | Passion fruit buccal tablet capable of lowering blood sugar level |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |