CN105392492B - Composition for preventing hair loss or promoting hair growth containing wheat bran extract as active ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth, which contains an extract of wheat bran as an active ingredient. The pharmaceutical composition containing the wheat bran extract as an active ingredient according to the present invention is recognized for its effects of inhibiting and preventing hair loss and promoting hair growth, and can be variously used in the fields of medicines, cosmetics, foods, beauty, and the like.

Description

Composition for preventing hair loss or promoting hair growth containing wheat bran extract as active ingredient

Technical Field

The present invention relates to a composition for preventing hair loss or promoting hair growth, containing an extract of wheat bran as an active ingredient.

Background

About 10 to 15 ten thousand hairs of a human body are formed in "hair follicles". The hair follicle has a papilla, in which small blood vessels are distributed to supply nutrients necessary for hair growth, and a cortical gland for supplying oil and fat for hair wetting is present near the top of the papilla.

The individual hairs have mutually different cycles, and grow and fall through a growth period (anagen), a catagen (catagen), and a telogen (telogen). The period is repeated after 3-6 years, and the average hair of 50-100 hairs is normally shed every day.

Alopecia is usually referred to as alopecia because of a short proportion of hairs in the anagen phase, many hairs in the catagen or telogen phase, and an abnormally large number of hairs that are shed.

The causes of alopecia include male hormone hyperactivity, sebum hypersecretion, poor blood circulation, scalp hypofunction caused by peroxides, bacteria, etc., genetic factors, aging, stress, etc., which are frequently mentioned.

Specifically, testosterone (testosterone), which is one of male hormones, is activated by an enzyme called 5 α -reductase (5 α -reductase) to Dihydrotestosterone (DHT), which binds to a specific receptor to induce a protein that causes alopecia, resulting in alopecia, and based on this mechanism, excess sebum is produced to cause acne, seborrheic dermatitis, and the like, and alopecia accompanied with inflammation appears on the scalp.

Minoxidil (minoxidil), mucopolysaccharide auxin (tricholaccharide), finasteride (finasteride) and the like have been developed as a vasodilator for treating hypertension at first but have been developed as a hair-growing agent because of its reported side effects of hirsutism.

Korean laid-open patent No. 2010-0111640 describes hair loss prevention and hair growth promotion effects of a composition comprising one or more selected from the group consisting of brown rice, black rice and rice. However, there is no description in any of the prior art documents about the hair loss preventing and hair growth promoting effects of wheat bran.

In this regard, the present inventors have found that a wheat bran extract plays an important role in hair growth, and have continued research to complete the present invention.

Disclosure of Invention

Technical problem to be solved by the invention

The present invention aims to provide a pharmaceutical composition containing an extract of wheat bran as an active ingredient, which is safe to the skin, and can prevent alopecia and promote hair growth.

Another object of the present invention is to provide a cosmetic composition containing an extract of wheat bran as an active ingredient.

Another object of the present invention is to provide a functional health food containing an extract of wheat bran as an active ingredient.

Another object of the present invention is to provide a method for preventing hair loss and promoting hair growth, comprising the step of treating a hair loss site with a pharmaceutical composition comprising an extract of wheat bran.

Another object of the present invention is to provide a use of an extract from wheat bran for preparing a pharmaceutical composition for preventing hair loss and promoting hair growth.

Means for solving the problems of the present invention

The present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth, which contains an extract of wheat bran as an active ingredient.

The content of the wheat bran extract is 0.01-50% by weight relative to the total weight of the composition, but is not limited thereto. If the amount is outside the above range, the effects of preventing hair loss and promoting hair growth may not be achieved.

The pharmaceutical composition may be selected from the group consisting of tablets, capsules, injections, creams, gels, patches, sprays, ointments, plasters, lotions, liniments, pastes and poultices.

From another aspect, the present invention provides a cosmetic composition for preventing hair loss or promoting hair growth, comprising an extract of wheat bran as an active ingredient.

The content of the wheat bran extract is 0.01-50% by weight relative to the total weight of the composition, but is not limited thereto. If the amount is outside the above range, the effects of preventing hair loss and promoting hair growth may not be achieved.

The cosmetic composition may be in a form selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair tonic, shampoo, hair rinse, hair treatment, hair cream, hair tonic, hair cream, hair massage cream, hair wax, hair aerosol, hair mask, hair tonic, hair soap, hair cleansing foam, hair oil, hair drying agent, hair conditioner, hair dye, hair curling preparation, hair bleach, hair gel, hair glaze, hair cosmetics, hair sticker, hair moisturizer, hair mousse, and hair spray.

From another aspect, the present invention provides a functional health food for preventing hair loss or promoting hair growth, comprising an extract of wheat bran as an active ingredient.

The term "health functional food" as defined in the present invention means a food prepared and processed from functional materials or ingredients which are useful for the human body according to the law No. 6727 concerning health functional foods, and the term "functional" means a food which is ingested for the purpose of regulating nutrients or obtaining health effects such as physiological actions for the structure and functions of the human body.

The content of the wheat bran extract is 0.01-50% by weight relative to the total weight of the composition, but is not limited thereto. If the amount is outside the above range, the effects of preventing hair loss and promoting hair growth may not be achieved.

The health functional food may be a powder, a granule, a tablet, a capsule or a beverage.

From another aspect, there is provided a method for preventing hair loss and promoting hair growth, comprising the step of treating a hair loss site with a pharmaceutical composition containing an extract of wheat bran.

From another aspect, there is provided a use of an extract of wheat bran for preparing a pharmaceutical composition for preventing hair loss and promoting hair growth.

Effects of the invention

The composition containing the wheat bran extract as an active ingredient has the effects of inhibiting and preventing alopecia and promoting hair growth. The composition containing a wheat bran extract of the present invention can be used in various fields such as pharmaceuticals, cosmetics, foods, and cosmetics.

Drawings

Figure 1 shows the dermal papilla cell (dermal papilla cell) proliferation efficacy of the wheat bran hexane fraction.

FIG. 2 is a graph showing the appearance of cyclin and Wnt/β -catenin signaling protein on dermal papilla cells by the wheat bran hexane fraction.

Fig. 3 shows the hair fiber (hair-fiber) growth effect of the wheat bran hexane fraction in the nose hair follicle (vibrisa folliculles).

Fig. 4 is a graph showing the confirmation of hair growth effect by the wheat bran hexane fraction in vivo.

A: hair growth effect over time for the control group, the hexanes fraction treated group, and the minoxidil treated group (MINOXYLTM) on mice; and

b: skin area (black part)%, relative to the long hair of the whole skin.

Detailed Description

The present invention is described in detail below.

In the present invention, "wheat (Triticum aestivum L.)" refers to an annual plant belonging to the gramineae family of the order oryzales, which is a monocotyledonous plant. It is known that wheat has excellent anticancer and antioxidant effects.

The "wheat bran" is the fraction remaining after the flour and germ have been separated from the grist, mostly the seed coat, but still containing a small amount of endosperm, germ.

The "extract" as defined in the present invention includes an extract soluble in a solvent selected from water including purified water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, diethyl ether, chloroform, preferably water or a mixed solvent of methanol.

The "wheat bran extract" is not particularly limited as long as it is a product obtained by extracting an active ingredient from wheat bran. For example, the wheat bran is put in water or an organic solvent, and the active ingredient is eluted by standing, stirring, pressurizing, heating or the like. Further, a freeze-dried product obtained by freeze-drying the liquid extract obtained in the above manner is also included.

Further, the freeze-dried product may be pulverized to obtain a powder. In addition, the extract obtained by any means capable of extracting the effective components by those skilled in the art, and the product obtained by freeze-drying or the like after extraction are also included. The extract may be an extract obtained by a conventional extraction method such as decoction or extraction at room temperature, or an extract obtained by a general extraction method described in korean medical books or textbooks.

Also, fractionated extracts obtained by employing a specific extraction method for isolating a specific active compound, i.e., the Stass Otto extraction method, various column chromatography (column chromatography) methods, or the like, are also included.

The wheat bran extract may be collected, commercially available, or the like without limitation, and may be used by washing with water after collection to remove foreign matter and then drying.

The wheat bran extract can be obtained by a common extraction method in the art such as an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like.

Preferably, the extract is obtained by extracting testa Tritici with solvent selected from water, C1-4 lower alcohol or their mixture, hexane, butanediol, propylene glycol, glycerol, ethyl acetate, diethyl ether, and chloroform.

"alopecia" refers to the phenomenon of hair falling from the scalp or the state of hair becoming thin or thin. "preventing alopecia" means preventing and inhibiting the phenomenon of alopecia as described above. "promoting hair growth" means not only promoting new hair growth but also healthy growth of existing hair.

The hair cycle can be divided into 3 main steps of a growth period, a return period and a rest period. In the anagen phase, with the rapid proliferation of cells, hair follicles grow deeply into the skin to form hairs. The next phenomenon is the catagen, which is a prominent transitional phase in which the interruption of cell division occurs, during which the hair follicle gradually regresses and hair growth is interrupted. In the telogen phase of the next phenomenon, the degenerative hair follicles comprise embryos (germ) with densely packed dermal papilla (dermal papillala) cells. In the telogen phase, the onset of a new growth phase is induced by rapid cell proliferation of the embryo, expansion of the dermal papilla and synthesis of basement membrane elements.

In general, "hair growth" is developed in the anagen phase, promoted by induction from the telogen phase to the anagen phase and delay from the anagen phase to the catagen phase.

Next, it is necessary to prevent hair loss, that is, to prevent hair loss, or to induce regrowth of hair, that is, to promote hair growth, by promoting or extending the anagen phase.

The composition according to an embodiment of the present invention has one or more of an effect of preventing existing hair from falling off, an effect of improving existing hair such as thickening the existing hair, and an effect of generating new hair. In addition, the composition based on the present invention activates dermal papilla cells to induce hair growth.

In the specific example of the present invention, experiments were conducted to determine whether wheat bran hexane (hexane) fractionation has hair growth efficacy using culture of dermal papilla cells derived from hair follicles and rat nasal hair follicles (ravibrissa follilexes).

In one embodiment of the present invention, in order to confirm whether or not the effect of proliferation of dermal papilla cells is exhibited, an investigation was conducted using immortalized dermal papilla cells (rat fibroblast). Dermal papilla cell growth and proliferation increased by 115.1 + -5.2% (P <0.01), 139.3 + -5.4% (P <0.001), 190.5 + -6.5% (P <0.001), and 183.3 + -10.2% (P <0.0001) when wheat bran hexane fractions were treated at concentrations of 1, 10, 50, and 100 μ g/ml. The growth and proliferation effect of dermal papilla cells was higher than that of dermal papilla cells of minoxidil (minoxidil) used as a positive control substance.

In one embodiment of the present invention, it was confirmed that proteins involved in signal transmission pathways, which play important roles in hair growth and regulation of cell proliferation, appear in dermal papilla cells. It is well known that mammalian cells require activation of cyclin E/CDK2complex, increased cyclin D1 and increased phosphorylation of pRB for the major process of cell proliferation, i.e., cell cycle progression, etc. (Sherr, C.J. (1996) Science274: 1672-. Minoxidil is known to increase the level of dermal papilla cells by cyclin E and CDK2, p27^kip1The level is reduced to promote cell cycle progression, proliferation of dermal papilla cells is induced (Kang, j.i., Kim, e.j., Kim, m.k., Jeon, y.j., Kang, s.m., Koh, y.s., Yoo, e.s.and Kang, H.K (2013), mar.drug 11: 1783. other.)) and the Wnt/β -catenin signaling pathway plays an important role in hair growth, regulation of cell proliferation (Kwack, m.h., Kang, b.m., Kim, m.k., Kim, j.c.and Sung, Y.K. (2011), j.dermotol.sci.62: 154. D.159. of Digend, ngefjord, s. stedt, braedson, J.p. and Y.K, Y.K. J.d.p., J.d.8. 12. 94. 12. d.p. and the like, after phosphorylation of cells, the cell cycle is induced by the level of stem cells, activation of stem cells, the stem cell cycle is induced by the level reduction, activation of stem, the stem, stem cells, the stemOn the other hand, KWack et al suggested that minoxidil showed hair growth efficacy in human DPCs through modulation of the activated β -catenin signaling pathway through PKA, Akt and GSK3 β.

In addition, in one embodiment of the present invention, 3-week-old mice were cultured for nasal hair follicles (rat vibrissa folliles), and the hair growth efficacy of the wheat bran hexane fraction-treated group was investigated. In particular, the hexane fraction treated group of wheat bran showed higher growth efficacy of hair fibers (hair-fiber) than 10 μ M minoxidil as a positive control group at concentrations of 10 and 50 μ g/ml.

The examples show that the wheat bran extract has excellent effects of preventing alopecia and promoting hair growth.

In conclusion, the wheat bran extract of the present invention has the following effects: promote the growth and proliferation of dermal papilla cells, which play a very important role in hair growth, and induce or maintain the anagen phase of hair follicles.

The pharmaceutical composition of the present invention containing the wheat bran extract as an active ingredient may be used in the form of a tablet, capsule, injection, cream, gel, patch, spray, ointment, plaster, lotion, liniment, paste or poultice for preventing alopecia and promoting hair growth, but is not limited thereto.

The preferred dose of the wheat bran extract of the present invention may vary depending on the state and body weight of the patient, the severity of the disease, the type and route of use of the drug and the duration, and can be appropriately selected by those skilled in the art. However, to achieve the preferred effect, the extract of the present invention may be administered daily at a dose of 0.0001 to 100mg/kg, preferably 0.001 to 10 mg/kg. The administration may be carried out once daily or in divided doses per day. Thus, the dosages are not intended to limit the scope of the invention in any respect.

In addition, the wheat bran extract of the present invention can be variously used in cosmetics having effects of preventing hair loss and activating hair. Examples of products to which the present composition can be added include cosmetics such as hair tonic, hair conditioner, hair essence, hair lotion, hair tonic, shampoo, hair rinse, hair cream, hair tonic, hair cream, hair massage cream, hair wax, hair aerosol, hair mask, hair tonic, hair soap, hair cleansing foam, hair oil, hair drying agent, hair conditioner, hair dye, hair curling preparation, hair bleach, hair gel, hair glaze, hair dressing, hair setting product, hair rinse, hair moisturizer, hair mousse, hair spray, and the like.

The cosmetic of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular peptides, high molecular polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamins are not particularly limited as long as they can be mixed with cosmetics, and preferably include vitamin B1, vitamin B2, vitamin B6, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinamide, folic acid, vitamin C, vitamin H, and the like, and salts (thiamine hydrochloride, sodium ascorbate, and the like) or derivatives (sodium ascorbyl-2-phosphate, magnesium ascorbyl-2-phosphate, and the like) of the above components are also included in the water-soluble vitamins that can be used in the present invention. The water-soluble vitamin can be obtained by a common method such as a microbial transformation method, a purification method in which purification is performed from a culture solution of a microorganism, an enzymatic method, or a chemical synthesis method.

As the oil-soluble vitamin, any oil-soluble vitamin may be used without limitation as long as it can be mixed with cosmetics, and preferably, vitamin a, carotene, vitamin D2, vitamin D3, vitamin E (D1-tocopherol, D-tocopherol) and the like are mentioned, and derivatives of these components (ascorbyl palmitate, ascorbyl stearate, ascorbyl dipalmitate, dl-tocopherol acetate, dl-tocopherol nicotinate, vitamin E, DL-panthenol, D-panthenol, panthenyl ethyl ether and the like) and the like are also included in the oil-soluble vitamin used in the present invention. The oil-soluble vitamin can be obtained by a common method such as a microbial transformation method, a purification method in which purification is performed from a culture solution of a microorganism, an enzymatic method, or a chemical synthesis method.

The polymer peptide may be used without limitation as long as it can be mixed with a cosmetic. Preferred examples thereof include collagen, hydrated collagen, gelatin, elastin, hydrated elastin, keratin and the like. The polymer peptide can be obtained by purification by a common method such as purification from a culture solution of a microorganism, an enzymatic method, a chemical synthesis method, or the like, or can be generally used by purification from natural products such as dermis of pig, cow, or the like, silk fibroin derived from silkworm, or the like.

The polymer polysaccharide may be used without limitation as long as it can be mixed with a cosmetic. Preferred examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate and salts thereof (such as sodium salt). For example, chondroitin sulfate or a salt thereof can be used by purifying it from a normal mammal or fish.

The sphingolipid can be used without limitation as long as it can be mixed with a cosmetic. Preferably, ceramide, phytosphingosine, glycosphingolipid and the like are mentioned. The sphingolipid can be obtained by purifying from mammals, fishes, shellfishes, yeasts, plants, or the like by a usual method, or by chemical synthesis.

The seaweed extract may be used without limitation as long as it can be mixed with a cosmetic. Preferably, brown algae extract, red algae extract, green algae extract, and the like are mentioned, and carrageenan, alginic acid, sodium alginate, potassium alginate, and the like purified from the seaweed extract are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained from the seaweed by purification using a usual method.

The cosmetic composition of the present invention may contain, in addition to essential ingredients, other ingredients which can be blended into conventional cosmetic compositions, if necessary.

In addition to the above ingredients, the mixing ingredients to be added thereto may include fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, herbal extracts, pH adjusters, alcohols, colorants, fragrances, blood flow stimulants, coolants, antiperspirants, purified water, and the like.

The fat component may comprise ester fat, hydrocarbon fat, silicone fat, fluorine fat, animal fat, vegetable fat, etc.

Examples of the ester fat include tri-2-ethylhexylglyceride, 2-ethylhexylcetyl ester, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isopropyl pivalate, glyceryl tri (caprylic acid, capric acid), tri-2-ethylhexyltrimethylolpropane, triisotrimethylolpropane stearate, tetra-2-ethylhexylpentaerythritol, cetyl octanoate, dodecyl laurate, hexyl laurate, dodecyl myristate, Myristyl myristate, cetyl myristate, stearyl stearate, lauryl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexane cetostearyl, 2-ethylhexane stearyl, hexyl isostearate, ethylene glycol dicaprylate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (caprylic acid, capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dicaprylate, glyceryl tricaprate, glyceryl triundecanoate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, glyceryl dicaprylate, glyceryl monostearate, glyceryl stearate, glyceryl sebacate, glyceryl stearate, Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate, polyglycerol isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesterol stearate, cholesterol isostearate, cholesterol hydroxystearate, cholesterol oleate, cholesterol dihydrooleate cholesteryl oleate, Phytosterol isostearate, phytosterol oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, isostearyl 12-stearoylhydroxystearate, and the like.

Examples of the hydrocarbon fat include squalene, liquid paraffin, α -olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based fat include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylstearyloxysiloxane copolymer, alkyl-modified silicone oil, amino-modified silicone oil, and the like.

Examples of the fluorine-containing fat include perfluoropolyether.

Examples of the animal or vegetable fat include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, safflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, coconut oil, wheat germ oil, rice germ oil, shea butter, evening primrose oil, macadamia nut oil, meadowfoam seed oil, egg yolk oil, beef tallow, horse oil, mink oil, orange roughy oil, jojoba oil, candelilla wax, carnauba wax, liquid lanolin, hydrogenated castor oil, and the like.

Examples of the moisturizer include a water-soluble low-molecular moisturizer, a fat-soluble low-molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer. Examples of the water-soluble low-molecular-weight moisturizer include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1, 3-butylene glycol, ethylene glycol, polyethylene glycol B (having a polymerization degree (n) of at least 2), polypropylene glycol (having a polymerization degree (n) of at least 2), polyglycerol B (having a polymerization degree (n) of at least 2), lactic acid, lactate, and the like.

Examples of the fat-soluble low-molecular-weight moisturizer include cholesterol and cholesterol ester. Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth gum, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, reduced mannonic acid (chitonic acid), dextrin, and the like.

Examples of the fat-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, polymer silicon, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesterol ester, cholesterol hydroxystearate, 12-hydroxystearic acid, stearic acid, abietic acid, and lanolin fatty acid cholesterol ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self-emulsified glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, Polyoxyethylene (POE) sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE/POP (polyoxyethylene-polyoxypropylene) copolymer, POE/POP alkyl ether, polyether-modified silicon, lauric alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, and the like.

Examples of the anionic surfactant include fatty acid soap, α -acyl sulfonate, alkyl sulfonate, alkylaryl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkanoylalkyl taurine, N-acylamino acid salt, POE alkyl ether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrated collagen peptide salt, and perfluoroalkyl phosphate ester.

Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, and quaternary ammonium salt derivatives of lanolin.

Examples of the amphoteric surfactant include carboxybetaine type, amidobetaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type amphoteric surfactants, and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silica, magnesium silicate, talc, sericite, mica, kaolin, rouge, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, hemite, and a composite thereof; organic pigments such as polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silica resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene/styrene copolymer, silk powder, cellulose, CI pigment yellow or CI pigment orange; and composite pigments of inorganic pigments and organic pigments.

Examples of the organic powder include metal soaps such as calcium stearate, metal alkylphosphates such as sodium cetylate, zinc laurate and calcium laurate, metal salts of polyvalent acylamino acids such as calcium N-lauroyl- β -alaninate, zinc N-lauroyl- β -alaninate and calcium N-lauroyl glycinate, metal salts of polyvalent amidosulfonates such as calcium N-lauroyl-taurate and calcium N-palmitoyl-taurate, N-acyl basic amino acids such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoyl lysine, N- α -palmitoyl ornithine, N- α -lauroyl arginine and N- α -hydrogenated tallow fatty acid acyl arginine, N-acyl polypeptides such as N-lauroyl glycinyl glycine, α -amino fatty acids such as α -aminocaprylic acid and α -aminolauric acid, polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, tetrafluoroethylene, and the like.

Examples of the UV absorber include p-aminobenzoic acid, ethyl-p-benzoate, amyl p-aminobenzoate, octyl p-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, trimethylcyclohexyl salicylate, benzyl cinnamate, 2-ethoxyethyl p-methoxycinnamate, octyl p-methoxycinnamate, mono-2-ethylhexanetriyl di-p-methoxycinnamate, isopropyl p-methoxycinnamate, a diisopropyl/diisopropyl cinnamate mixture, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, sodium dihydroxybenzophenonedisulfonate, sodium dihydroxybenzophenone disulfonate, and the like, Dihydroxybenzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4 ' -methoxydibenzoylmethane, 2, 4, 6-trianilino-p- (carbonyl-2 ' -ethylhexyl-1 ' -oxy) -1, 3, 5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole and the like.

Examples of the bactericide include hinokitiol, triclosan (triclosan), triclosan, chlorhexidine gluconate, phenoxyethanol, resorcinol, isopropylmethylphenol, azulene (azulene), salicylic acid, zinc pyrithione, benzalkonium chloride, photosensitizer 301, sodium mononitroguaiacolate, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, propyl gallate, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, and disodium hydrogen phosphate.

Examples of the Alcohol include higher alcohols such as Cetyl Alcohol (Cetyl Alcohol).

Further, the mixed ingredients that can be added thereto are not limited to the above-mentioned ingredients, and any ingredients may be mixed within a range that does not hinder the object and effect of the present invention, the ingredients being preferably mixed in an amount of 0.01 to 50% by weight based on the total weight of the composition.

The cosmetic of the present invention may take the form of a solution, emulsion, viscous mixture, or the like.

As the active ingredient contained in the cosmetic composition of the present invention, in addition to the above-mentioned extract, ingredients generally used in cosmetic compositions, such as conventional adjuvants and carriers, for example, stabilizers, solubilizers, vitamins, pigments, and fragrances, may be contained.

The cosmetic composition of the present invention can be prepared into any preparation generally prepared in the field, and examples thereof include a lotion, a cream, a lotion, a pack, a foundation cream, a lotion, a essence, a hair care composition, and the like.

When the formulation of the present invention is in the form of a paste, cream or gel, as a carrier ingredient, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth (tragant), a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide may be used.

When the formulation of the present invention is in the form of a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier ingredient. Especially when the formulation of the invention is in the form of a spray, the formulation may also contain propellants such as chlorofluorocarbons, propane/butane or dimethylether.

When the preparation of the present invention is in the form of a solution or an emulsion, as a carrier ingredient, a solvent, a solvating agent or an emulsifying agent can be used, and for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butyl glycol oil, aliphatic glyceride, polyethylene glycol or sorbitol fatty acid ester can be mentioned.

When the formulation of the present invention is in the form of a suspension, as a carrier ingredient, a liquid diluent such as water, ethanol or propylene glycol; suspensions of ethoxylated isostearyl alcohols, polyethylene oxide sorbitol esters and polyethylene oxide sorbitan esters; microcrystalline cellulose, aluminum hydroxide oxide, bentonite, agar, or tragacanth, and the like.

When the formulation of the present invention is in the form of a surfactant-containing cleanser, as a carrier ingredient, fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazole derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolein derivative, ethoxylated glycerol fatty acid ester, or the like can be used.

The present invention also provides a functional health food for preventing hair loss or promoting hair growth, which contains the wheat bran extract as an active ingredient.

Examples of foods to which the wheat bran extract of the present invention can be added include various foods, beverages, chewing gums, teas, vitamin complexes, health functional foods, and the like, and can be used in the form of powders, granules, tablets, capsules, or beverages.

The wheat bran extract of the present invention has no toxicity or side effect, and can be used safely for a long-term use for the purpose of prevention.

The wheat bran extract of the present invention can be added to foods or beverages for the purpose of preventing and treating alopecia. In the case of the health functional food composition of the present invention, the wheat bran extract in the food or beverage may be generally added in an amount of 0.01 to 50% by weight based on the total weight of the food. In addition, in the case of a health drink composition, it may be added in a proportion of 0.02 to 10g, preferably 0.3 to 1g, per 100 ml.

The health drink composition of the present invention comprises the above extract as an essential component in a predetermined ratio, and other liquid components other than the above are not particularly limited, and may contain various additives such as a flavoring agent and a natural carbohydrate as in a general drink. Examples of the above natural carbohydrates include monosaccharides (e.g., glucose), disaccharides (e.g., fructose), polysaccharides (e.g., maltose or sucrose), conventional sugars (e.g., dextrin or cyclodextrin), and sugar alcohols (e.g., xylitol, sorbitol or erythritol). In addition to the above ingredients, as the flavoring agent, natural flavoring agents (thaumatin, stevia extract, rebaudioside a, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. As the above natural carbohydrate, it may be generally present in an amount of about 1 to 20g, preferably about 5 to 12g, based on 100ml of the composition of the present invention.

In addition to the above ingredients, the compositions of the present invention may contain various nutrients, vitamins, minerals (electrolytes), flavoring agents (e.g., synthetic or natural flavoring agents), coloring agents and modifiers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, and carbonating agents used in carbonated beverages, and the like. In addition, the composition of the present invention may comprise pulp for preparing natural fruit juices, fruit juice beverages, and vegetable beverages. These ingredients may be used alone or in combination. The amount of such additives is not critical but is generally selected from the range of 0 to about 20 parts by weight based on 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail with reference to examples for easy understanding. However, the following examples are only for illustrating the present invention and are not intended to limit the scope of the present invention to the examples. The embodiments of the present invention are provided for complete description to those skilled in the art.

Detailed Description

< example 1> obtaining and extraction of wheat bran

The wheat bran used in the present invention is wheat bran of wheat of China cultivated in northern Qingshang of Korea obtained from rice production. 5kg of total wheat bran samples were extracted by decocting with 90% ethanol in Water Bath (Water Bath) 3 times every 4 hours. Thereafter, the upper layer liquid was recovered, and concentrated under reduced pressure using a rotary vacuum concentrator (rotary evaporator), to obtain 337g of a crude extract. Of these, 300g of the sample was subjected to solvent fractionation using n-hexane (n-hexane) to obtain 133g of an n-hexane layer, and the fraction was used as a sample for an experiment.

< example 2> proliferation efficacy of dermal papilla cells

< example 2-1> confirmation of proliferation of dermal papilla cell

Immortalized dermal papilla cells (Rat vibrisa immortal dermal papilla cells) isolated from white mouse beard were cultured in DMEM (Hyclone Inc, USA) medium containing 100units/ml penicillin-100. mu.g/ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovine serum (Heat-inactivated fetal bovine serum server, FBS; GibcoInc, NY, USA) at 37 ℃ in 5% CO₂Culturing in a constant temperature machine, and carrying out passage every 3 days.

Proliferation of dermal papilla cells (dermal papilla cells) was determined using MTT Assay. Dermal papilla cells (dermal papilla cells) 1.0X 10 in DMEM medium containing 1% FBS⁴cells/ml were turbid, placed in a 96-well plate (well plate), and after 24 hours of incubation, the wheat bran hexane was fractionated at concentrations of 0.1, 1, 10, 50 and 100. mu.g/ml. Minoxidil (minoxidil, Sigma, MO, USA) as a positive control group was treated with a concentration of 10. mu.M.

After 4 days of culture, 50. mu.l of MTT (Sigma, MO, USA) was added and the reaction was allowed to proceed for 4 hours. The supernatant was removed, DMSO was added in an amount of 200. mu.l, and after the pellet was dissolved, the absorbance was measured at 540nm using a VERSAmax microplate reader (VERSAmax microplate reader, Molecular Devices, CA, USA). The average absorbance value of each sample addition group was calculated and compared with the absorbance value of the control group to investigate the degree of proliferation.

All the measurement results are expressed as mean ± standard deviation, and the statistical significance test was performed using Student's t-test (Student's t-test), and the significance was confirmed in the case where the p-value was 0.05 or less. The statistical processing used SPSS 12.0K (Release 12.0.1.SPSS inc. usa) of the Windows operating system.

As a result, when the wheat bran hexane fractions were treated at concentrations of 0.1, 1, 10, 50 and 100 μ g/ml, the proliferation of dermal papilla cells (dermal papilla cells) increased to 106.9 ± 11.5%, 115.1 ± 5.2% (P <0.01), 139.3 ± 5.4% (P <0.001), 190.5 ± 6.5% (P <0.001) and 183.3 ± 10.2% (P <0.001), respectively, compared to the control group (table 1, fig. 1).

[ TABLE 1 ]

In particular, the proliferation effect of the test group treated with the wheat bran hexane fraction, i.e., 112.3 ± 2.7% (P <0.01), showed higher proliferation efficacy than that of 10 μ M minoxidil used as a positive control group at concentrations of 10, 50 and 100 μ g/ml, and the wheat bran hexane fraction showed similar hair growth efficacy to that of minoxidil.

< example 2-2> confirmation of cell cycle protein expression in dermal papilla cells

To confirm the molecular mechanism by which the wheat bran hexane (hexane) extract increases growth and proliferation of dermal papilla cells, the development of cell cycle (cell cycle) proteins was observed. Specifically, Dermal papilla cells (Dermal papillacell) (1.0X 10) were cultured in DMEM medium containing 1% FBS⁵cell/ml in 100mm dish) for 24 hours, and then the wheat bran extracts (1, 10 and 50. mu.g/ml) and 10. mu.M minoxidil were treated, respectively, for 24 hours. After washing the cells 2 times with PBS, 200. mu.l of lysine buffer [50mM Tris-HCl (pH 7.5),150mM NaCl,2mM EDTA,1mM EGTA,1mM NaVO3,10mM NaF,1mM Dithiothioredol (DTT),1mM phenylmethylisul fonylfluoride (PMSF), 25. mu.g/mL aprotinin, 25. mu.g/mL leupeptin and 1% NP-40 ] were added]Then, the cells were pulverized at 4 ℃ for 30 minutes. The cell lysate (lysate) was centrifuged at 15,000rpm for 15 minutes to obtain an upper layer solution, which was stored at-20 ℃ until used in the experiment. Protein concentration bsa (bone serum albumin) was quantified using standard water using the coomassie brilliant blue method (Bradford) method. Denaturing and separating 20-30. mu.g of cell lysate with 8-12% mini gel SDS-PAGE (sodium dedocyl sulfate polyacrylamide gel electrophoresis),after 2 hours of reaction with PVDF (polyvinylidene fluoride) membrane (Bio-Rad, Hercules, Calif., USA) in 200mA transfer (transfer) solution, blocking of the membrane was carried out for 2 hours in Tween-20-TBS (T-TBS) (50mM Tris, pH 7.6,150mM NaCl, 0.1% Tween-20) solution containing 5% nonfat drieldilk, after which antibodies to Cyclin D45 (1: 1000), phospho-2 (1: 1000), Cyclin E (1: 2000), phospho (ser780) -pRB (1: 1000), phospho-ERK1/2 (1: 1000), ERK 1/dyn (1: 1000), phospho (ser) -2-carban (1: 1000), phospho- β/2 (1: 1000), anti-phospho antibody (IgG) (Biocide, Hercules, Calif., USA) and anti-phospho antibody (Biocide) were added to the membrane after once dilution with GS7 mM Tris, pH 567 mM NaCl, 0.1.1% Tween-20, after reaction for 2 hours, after the addition of antibody to the membrane, phospho-5% serum D45 (1: 1000), binding to the antibody to 12, after once (Biocide, Biocide for 2, Biocide, 1: 1000, and anti-5, and anti-15, and anti-20, and Biocide for 2, and after the binding to the antibody to react with the membrane after the antibody to.

As a result, when dermal papilla cells were treated with the wheat bran hexane extract at concentrations of 1, 10 and 50 μ g/ml for 24 hours, it was confirmed that the expression of cyclin D1, cyclin E, phosphor-CDK 2 and phosphor-pRB was increased, and that the treatment with minoxidil showed similar results to the wheat bran hexane extract (fig. 2A), and that the treatment with wheat bran hexane extract showed an increase in activation of Phospho (ser552) - β -catenin, Phospho (ser675) - β -catenin, Phospho (ser9) -GSK3 β, and that the treatment with minoxidil was able to activate much of the Wnt/β -catenin pathway (fig. 2B), and that the wheat bran hexane extract showed an increase in the proliferation activity of proteins not only through cyclin D1, cyclin E, phosphor-CDK 2, phosphor-493b, etc., but also through the proliferation pathway of proteins, Wnt a-Akt 23, and the proliferation pathway of dermal papilla cells.

< example 3> hair growth efficacy assay: hair fiber (Hair-fiber) Length growth assay

< example 3-1> isolation and culture of nasal hair follicles (Rat vibrissa follicles) of rats

Three-week-old male Wistar rats (Japan SLC, Hamamatsu, Japan) were purchased from central laboratory animals, anesthetized with ethyl ether, and sacrificed by cervical dislocation. Left and right facial whisker pads (mystatic pads) of rats (Rat) were removed and placed in E/P buffer ((Earle's balanced lipids solution (EBSS, Sigma MO, USA) + phosphate-buffered saline (PBS, SigmaMO, USA)) supplemented with 100units/ml penicillin and 100. mu.g/ml streptomycin (streptomycin, Gibco Inc, NY, USA). after complete separation of the follicles under a stereomicroscope, the follicles were placed in Petri dishes containing E/P buffer and incubated at 37 ℃ with 5% CO₂Incubate in a thermostat for 1 hour. Mu.l of William E medium (William E medium, Gibco Inc, NY, USA) containing 2 mML-glutamine (Gibco Inc, NY, USA), 10. mu.g/ml insulin (Sigma MO, USA), 50nM hydrocortisone (hydrocortisone, Sigma MO, USA) and 100units/ml penicillin-100. mu.g/ml streptomycin was added to each well of a 24-well plate, one follicle was added to each well, 5% CO at 37 ℃₂Culturing in a thermostat. During the cultivation, the medium was replaced with fresh medium every 3 days, and the wheat bran hexane was fractionated at concentrations of 1, 10 and 50. mu.g/ml. Minoxidil as a positive control was treated at a concentration of 10. mu.M (Kang et al, 2012Biomol Ther20(1), 118-124).

< example 3-2> measurement of growth of nose hair follicle (Vibrissa follicles)

The shape of the nasal hair follicle (vibrissa folliculle) in culture was photographed using a microscope (Olympus, Japan). The length of the hair follicle was measured using an image analyzer (DP controller; Olympus, Japan). The average change in hair follicle length was calculated and compared to the average length of the control group to determine the length of growth.

All the measurement results are expressed as mean ± standard deviation, and the statistical significance test was performed using Student's t-test (Student's t-test), and the significance was confirmed in the case where the p-value was 0.05 or less. The statistical processing used SPSS 12.0K (Release 12.0.1.SPSS inc. usa) of the Windows operating system.

On day 21, the results of comparing the difference (%) in length of hair follicles between the mesothelial hexane-fractionated treated group and the control group increased the hair fiber growth to 150.0. + -. 21.7% (P <0.001), 203.6. + -. 7.6% (P <0.001) and 246.4. + -. 19.8% (P <0.001) compared to the control group when treated at 1, 10 and 50. mu.g/ml, respectively (Table 2, FIG. 3).

[ TABLE 2 ]

In particular, the hair fiber (hair-fiber) growth increasing effect of the wheat bran hexane fraction was higher than 156.3 + -11.5% (P <0.05) of minoxidil (10. mu.M) used as a positive control substance at concentrations of 10 and 50. mu.g/ml.

< example 4> hair growth efficacy assay: in vivo experiment

< example 4-1> Experimental animals

Female C57BL/6 mice, 6 weeks old, were purchased from Orientbio inc, acclimated in an animal feeding room environment for 1 week for experimentation. The animal feeding room was maintained at a temperature of 23 + -3 deg.C for 12 hours each day. The experimental animals were kept in isolation mouse cages and given experimental specimens freely. Experimental group 5 groups were set as control group, positive control group MINOXYL^TM(modern medicine) and wheat bran hexane extract (10, 50 and 100 μ g/ml). In one experimental group, 8 mice were used for the experiment, respectively.

< example 4-1> Hair growth efficacy assay

To test the hair growth effect, 7 weeks of age of resting body hair with a dorsal skin tone pink was used. All hairs on the back of the mouse were removed by a clipper using a small animal. Mu.l of the test material was dropped once a day to the hair removal part, and patted and further coated with 50% ethanol on the control group. After the start of the experiment, the hair growth state on day 1, day 15, day 22, day 29 and day 35 was confirmed, and an image was taken, and dotmatrix planimetry was performed for quantitative evaluation.

As a result, in the barley peel hexane extract-coated group, skin color change was observed from day 22 after hair removal, and skin color change and hair growth were observed on days 29 and 35 after hair removal. As a result of quantitative analysis of anagen phase-inducing efficacy of hair of the horridix extract-coated group using dotmatrix area assay, it was confirmed that the horridix extract-coated group showed superior anagen phase-inducing efficacy compared to the control group at a concentration of 50 μ g/ml on day 35 (fig. 4). In addition, the MINOXYLTM (containing 5% minoxidil) coated group as the positive control group was observed to have a change in skin color and a significant hair growth effect from day 15 after hair removal (fig. 4). The results indicate that the wheat bran hexane extract is able to induce the anagen phase of hair in vivo.

< example 5> statistical analysis

All measurements are expressed as mean ± standard deviation and tested for statistical significance using student's t-test. Here, when the p value is 0.05 or less, it is proved to be significant. Statistical processing used SPSS 12.0K for windows (Release 12.0.1.SPSS inc. usa).

In the present invention, the wheat bran hexane extract has been found to be useful as a therapeutic and prophylactic agent for alopecia, because it promotes the growth and proliferation of cells by activating cell cycle proteins and Wnt/β -catenin signaling pathways, and induces the anagen phase of hair follicles or maintains the anagen phase.

The following examples are provided to illustrate the present invention in more detail, and are not intended to limit the scope of the present invention.

Preparation example 1 preparation of Hair growth oil

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	10.0
Resorcinol (Resorcinol)	0.1
		Menthol (menthol)	0.05
Panthenol (panthenol)	0.2
		Salicylic acid (salicylic acid)	0.1
Tocopheryl Acetate (Tocopherol Acetate)	0.1
		Pyridoxine hydrochloride	0.1
Castor oil (caster oil)	5.0
		Pigment	Proper amount of
Perfume	Proper amount of
		Ethanol	Proper amount of
Purified water	Balance of
		Total up to	100

Preparation example 2 preparation of Hair conditioner

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	2.5
Cetyl alcohol	3.5
		Self-emulsifying glyceryl monostearate	1.5
Propylene glycol	2.5
		Stearyl methylbenzyl ammonium chloride (25%)	7.0
Para-oxybenzoic acid methyl ester	0.3
		Pigment	Proper amount of
Perfume	Proper amount of
		Purified water	Balance of
Total up to	100

Preparation example 3 preparation of Hair lotion

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	5.0
Resorcinol	2.0
		Panthenol	0.5
Piroctone Olamine (Piroctone Olamine)	0.1
		Pigment	Proper amount of
Perfume	Proper amount of
		Purified water	Balance of
Total up to	100

Preparation example 4 preparation of shampoo soap

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	0.1
Titanium dioxide	0.2
		Polyethylene glycol	0.8
Glycerol	0.5
		Ethylenediaminetetraacetic acid	0.05
Sodium salt	1.0
		Pigment	Proper amount of
Essence for soap	Proper amount of
		Cosmetic soap base (moisture 13%, weight portion)	Balance of
Total up to	100

Formulation example 5 hydrophilic ointment

Preparation example 6 preparation of tablet

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	33.3
Lactose	30
		Microcrystalline cellulose	13
Magnesium stearate	1
		Corn starch	Balance of
Total up to	100

According to a usual method for producing tablets, the above components are added in the above amounts, uniformly mixed and stirred, and then granulated. After drying, a tablet press was used to prepare a desired tablet containing 33.3 wt% of an active ingredient, i.e., an extract of wheat bran per tablet.

Preparation example 7 preparation of capsules

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	33
Lactose	30
		Microcrystalline cellulose	13
Magnesium stearate	1
		Corn starch	Balance of
Total up to	100

According to a usual method for producing capsules, the above components are added in the above amounts, mixed uniformly, and filled into gelatin capsules of an appropriate size so that 33% by weight of an active ingredient, i.e., a wheat bran extract, is contained in each capsule, thereby producing desired capsules.

Preparation example 8 preparation of liquid preparation

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	20
Isomerized sugar	10
		Mannitol	5
Purified water	Balance of
		Total up to	100

According to a general method for producing a liquid formulation, a liquid formulation is prepared by dissolving each of the above-mentioned ingredients in purified water, adding a lemon flavor to it, mixing all the ingredients, adding pure water to the resulting mixture so that the final amount of the mixture is adjusted to 100ml, filling the mixture in a brown vial and sterilizing the mixture.

Preparation example 9 preparation of health functional food

The composition ratio of the vitamin and mineral mixture is obtained by using the relatively suitable ingredients for the health functional food in the preferred experimental examples, but may be optionally changed. Subsequently, the above ingredients are mixed according to a conventional method for preparing a health functional food to prepare granules, and can be used for preparing a health functional food according to a conventional method.

Preparation example 10 preparation of health drink

Mixing the ingredients	Content (wt%)
		Wheat bran extract of example 1	50
Citric acid	0.15
		Liquid phase fructose	7
Fruit concentrate	14.4
		Taurine	2
Purified water	Balance of
		Total up to	100

According to a conventional method for preparing a health drink, the above ingredients are mixed and heated at 85 ℃ for about 1 hour with stirring. The resulting mixture was filtered and placed in a 2L sterile container. The sterile container is then sealed, sterilized and refrigerated. This mixture is then used to prepare the health drink composition of the present invention.

The compositional ratio of these ingredients is obtained by mixing relatively suitable ingredients for health beverages in the preferred embodiment, but may be optionally changed according to the country of the category and need, and the preference (e.g., use) of the region and country.

Claims (9)

1. A pharmaceutical composition for preventing hair loss or promoting hair growth comprises hexane fraction containing ethanol extract of wheat bran at a concentration of 10 μ g/ml to 100 μ g/ml as an effective ingredient.

2. The pharmaceutical composition according to claim 1, wherein the hexane fraction of the ethanol extract of wheat bran is contained in an amount of 0.01 to 50% by weight relative to the total weight of the composition.

3. The pharmaceutical composition according to claim 1 or 2, which is in a dosage form selected from the group consisting of tablets, capsules, injections, creams, gels, patches, sprays, ointments, plasters, lotions, liniments, pastes and poultices.

4. A cosmetic composition for preventing hair loss or promoting hair growth contains hexane fraction containing ethanol extract of wheat bran at a concentration of 10 μ g/ml to 100 μ g/ml as an effective ingredient.

5. The cosmetic composition according to claim 4, wherein the hexane fraction of the ethanol extract of wheat bran is contained in an amount of 0.01 to 50% by weight relative to the total weight of the composition.

6. The cosmetic composition for preventing hair loss or promoting hair growth according to claim 4 or 5, which is in a dosage form selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair tonic, shampoo, hair rinse, hair treatment gel, hair cream, hair moisturizer, hair massage cream, hair wax, hair aerosol, hair tonic, hair soap, hair cleansing foam, hair drying agent, hair conditioner, hair curling preparation, hair bleach, hair gel, hair glaze, hair mucilage, and hair mousse.

7. A health functional food for preventing alopecia and promoting hair growth contains hexane fraction containing ethanol extract of testa Tritici as effective component at a concentration of 10 μ g/ml to 100 μ g/ml.

8. The health functional food according to claim 7, wherein the hexane fraction of the ethanol extract of wheat bran is contained in an amount of 0.01 to 50% by weight with respect to the total weight of the composition.

9. The functional health food for preventing hair loss and promoting hair growth according to claim 7, which is a powder, granules, tablets, capsules or beverage.

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