KR20230140008A - Composition for preventing hair loss, promoting hair growth or improving scalp condition comprising pinitol as an active ingredient - Google Patents

Abstract

Pinitol (D-Pinitol, 3-O-methyl-D-chiro-inositol) of the present invention promotes cell proliferation in dermal papilla cells, which are important for hair growth, and expression of alkaline phosphatase, a major biomarker. And by proving the effect of suppressing active oxygen, it has the effect of preventing hair loss and improving scalp condition/promoting hair growth through proliferation of human dermal papilla cells, which are important for hair loss. Therefore, pinitol of the present invention can be used in cosmetics, quasi-drugs, health functional foods, or pharmaceutical compositions. This project (result) is the result of a regional innovation project based on local government-university cooperation conducted in 2021 with funding from the Ministry of Education and support from the National Research Foundation of Korea (2021RIS-001). This results was supported by "Regional Innovation Strategy (RIS)" through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE)(2021RIS-001).

Description

Composition for preventing hair loss, promoting hair growth or improving scalp condition comprising pinitol as an active ingredient}

The present invention relates to a composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing Pinitol (D-Pinitol, 3-O-methyl-D-chiro-inositol) as an active ingredient, which is used for cosmetics, quasi-drugs, and health. It can be used in functional foods or pharmaceutical compositions.

Human hair protects the head from sunlight, cold, and external shocks, and is also important from a cosmetic perspective. In a modern society that places importance on appearance, hair loss is the biggest obstacle to external expression and is recognized as a disease, so research and development in various fields are being conducted. Due to increased stress, westernized diet, and internal and external causes, hair loss symptoms appear in more than 30% of men in their 40s and 50s, and the prevalence of hair loss in women is estimated to be more than 5%. Additionally, the age at which hair loss occurs is decreasing to those in their 20s and 30s.

Hair follicles, a physiological organ in the body that produces hair, produce hair during the postnatal development process, including the anagen phase, when the generated hair actively grows, the catagen phase, when hair degenerates, and when hair falls out. It repeats the hair growth cycle, which is divided into the telogen phase, which lasts until the end, and the exogen phase, when hair loss occurs, and is involved in the growth, maintenance, and shedding of hair. Many studies have shown that the activity of these hair follicles is caused by dermal papilla cells, and in particular, the proliferation and differentiation of dermal papilla cells are primarily involved in the progression of the hair growth cycle and hair formation. During the growth phase, when hair is actively growing, active proliferation and differentiation of dermal papilla cells occur, hair growth stops, and during the catagen, telogen, and hair loss phases when hair loss occurs, these cells die.

Therefore, since hair growth and hair loss are closely related to the proliferation and death of dermal papilla cells, lengthening the growth phase by inducing their proliferation or suppressing cell death and shortening the catagen phase, telogen phase, and hair loss phase is expected to be a way to improve and treat hair loss. It is understandable. In addition, cell division and migration near the dermal papilla are closely related to hair growth. During the growth phase, new hair is generated from the dermal papilla. The cells are activated by various cytokines, hormones, etc., resulting in cell migration to the dermal papilla, thereby producing hair. affects the growth of Therefore, proliferation of dermal papilla cells is a very important factor in suppressing hair loss and hair growth.

Currently, drugs that promote hair growth that have been approved by the U.S. Food and Drug Administration (FDA) include Minoxidil and Finasteride. However, these drugs have a short duration of effect, and some side effects include decreased libido, a risk for pregnant women in the case of finasteride, hair loss that occurs again compared to before drug administration when drug administration is stopped, and hormonal imbalance. It has been reported that there is a need for the development of new natural hair loss prevention agents.

Meanwhile, Pinitol (D-Pinitol, 3-O-methyl-D-chiro-inositol) is a natural blood sugar control ingredient contained in legumes and pine needles, and is a structural isomer of myo-inositol (chiro-inositol). -inositol) is a substance with a methyl group attached to carbon 3 through an ether bond. Pinitol exists in a variety of plants, including legumes, that exist in nature. In particular, it has been reported to be present in large quantities in soybeans, which are in the spotlight as folk remedies, as well as soybeans, acanthophytes, silkworm powder, and Hovenia chinensis. Pinitol has been found to be an active ingredient present in various plants used as folk remedies for diabetics around the world.

Accordingly, the present inventors conducted intensive research to develop a composition for preventing hair loss, promoting hair growth, or improving scalp condition containing excellent pinitol as an active ingredient, and as a result, it was confirmed that pinitol is effective in preventing hair loss, promoting hair growth, or improving scalp condition. And thus, the present invention was completed.

This project (result) is the result of a regional innovation project based on local government-university cooperation conducted in 2021 with funding from the Ministry of Education and support from the National Research Foundation of Korea (2021RIS-001). This results was supported by "Regional Innovation Strategy (RIS)" through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE)(2021RIS-001).

10-2012-0087084

The purpose of the present invention is to provide a cosmetic composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

In addition, another object of the present invention is to provide a quasi-drug composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

In addition, another object of the present invention is to provide a health functional food composition containing pinitol as an active ingredient for preventing hair loss, promoting hair growth, or improving scalp condition.

Finally, another object of the present invention is to provide a pharmaceutical composition for preventing or treating hair loss containing pinitol as an active ingredient.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

In one embodiment of the present invention, the pinitol may be represented by Formula 1:

[Formula 1]

.

In one embodiment of the present invention, the composition may have an effect of improving wound healing of dermal papilla cells, but is not limited thereto.

In one embodiment of the present invention, the composition may increase the expression of alkaline phosphatase in dermal papilla cells, but is not limited thereto.

In one embodiment of the present invention, the composition may have an effect of suppressing free radicals in dermal papilla cells, but is not limited thereto.

Next, the present invention provides a quasi-drug composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

Furthermore, the present invention provides a health functional food composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

Finally, the present invention provides a pharmaceutical composition for preventing or treating hair loss containing pinitol as an active ingredient.

Pinitol (D-Pinitol, 3-O-methyl-D-chiro-inositol) of the present invention promotes cell proliferation in dermal papilla cells, which are important for hair growth, and expression of alkaline phosphatase, a major biomarker. And by proving the effect of suppressing active oxygen, it has the effect of preventing hair loss and improving scalp condition/promoting hair growth through proliferation of human dermal papilla cells, which are important for hair loss.

Figure 1 is a diagram showing the effect of D-pinitol on improving wound healing of human scalp-derived dermal papilla cells, which are hair loss cell models, in one embodiment of the present invention.
Figure 2 is a diagram showing the effect of D-pinitol on increasing the expression of the key biomarker alkaline phosphatase in human scalp-derived dermal papilla cells, which are a hair loss cell model, in one embodiment of the present invention.
Figure 3 is a diagram showing the effect of D-pinitiol on suppressing free radicals on human scalp-derived dermal papilla cells, which are hair loss cell models, in one embodiment of the present invention.

Hereinafter, the present invention will be described in detail through embodiments of the present invention with reference to the attached drawings. However, the following implementation examples are provided as examples of the present invention, and if it is judged that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. , the present invention is not limited thereby. The present invention is capable of various modifications and applications within the description of the patent claims described below and the scope of equivalents interpreted therefrom.

In addition, terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when it is said that a part “includes” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

Throughout this specification, '%' used to indicate the concentration of a specific substance means (w/w) % for solid/solid, (w/v) % for solid/liquid, and Liquid/liquid is (v/v) %.

In one aspect, the present invention provides a cosmetic composition for preventing hair loss, promoting hair growth, or improving scalp condition, comprising pinitol as an active ingredient.

The composition containing pinitol of the present invention can be used in various ways, such as preventing hair loss, promoting hair growth, or improving scalp condition. Products to which this composition can be added include, for example, cosmetics such as various creams, lotions, skins, and essences, as well as shampoos, rinses, cleansers, face washes, soaps, treatments, packs, and beauty essences.

The cosmetic of the present invention includes a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, sphingolipids, and seaweed extracts.

Water-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, and vitamin H. and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate, etc.) are also water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic methods, or chemical synthesis.

Oil-soluble vitamins may be any that can be mixed into cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. , their derivatives (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microbial cultures, enzymatic or chemical synthesis.

The polymer peptide may be any peptide that can be blended into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. High molecular weight peptides can be purified and obtained by conventional methods such as purification from microbial culture media, enzymatic methods, or chemical synthesis, or they can usually be purified and used from natural products such as dermis of pigs or cows or silk fiber of silkworms.

The polymer polysaccharide may be any one that can be blended into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or its salts (sodium salt, etc.). For example, chondroitin sulfate or its salt can usually be purified and used from mammals or fish.

The sphingolipid may be any one that can be blended into cosmetics, but preferably includes ceramide, phytosphingosine, and sphingolipid. Sphingolipids can usually be purified by conventional methods from mammals, fish, shellfish, yeast, or plants, or obtained by chemical synthesis.

The seaweed extract may be any one that can be blended into cosmetics, but preferably includes brown algae extract, red algae extract, green algae extract, etc. Also, calrageenan, arginic acid, sodium alginate, etc. purified from these seaweed extracts. Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above-mentioned essential ingredients, the cosmetic of the present invention may contain other ingredients usually blended in cosmetics as needed. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, disinfectants, antioxidants, plant extracts, pH adjusters, alcohol, colorants, fragrances, Examples include blood circulation promoters, cooling agents, antiperspirants, and purified water. Examples of oil and fat components include ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, fluorine-based fats and oils, animal fats and oils, and vegetable fats and oils.

Ester-based oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine. Diisopropyl acid, isoalkyl neopentanoate, tri(caprylic, capric acid)glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelislitol tetra2-ethylhexanoate , cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostear laurate. Reyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetoester 2-ethylhexanoate Aryl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanate, ethylene glycol dioleate, propylene glycol dicapric acid, di(capryl, capric acid) propylene glycol, propylene glycol dicaprylate, dicapric acid Neopentyl glycol prinate, neopentyl glycol dioctanate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostear octanoate. Reyl, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleic acid ester, polyglycerol isostearic acid ester. , Triisocetyl citrate, Triisoalkyl citrate, Triisooctyl citrate, Lauryl lactate, Myristyl lactate, Cetyl lactate, Octyldecyl lactate, Triethyl citrate, Acetyltriethyl citrate, Acetyltributyl citrate, Trioctyl citrate, Mal. Diisostearyl acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate. , cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytsteryl isostearate, phytsteryl oleate, 12-stealoyl hydroxystearate isocetyl, 12-stealoyl Ester systems such as stearyl monohydroxystearate and isostearyl 12-stealoylhydroxystearate can be mentioned.

Hydrocarbon-based fats and oils include squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybudene, microcrystalline wax, and petroleum jelly.

Silicone oils include polymethyl silicone, methylphenyl silicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methylstealoxysiloxane copolymer, and alkyl. Modified silicone oil, amino-modified silicone oil, etc. can be mentioned.

Examples of fluorine-based fats and oils include perfluoropolyether.

Animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, birdflower oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, and grape seed oil. , cottonseed oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum oil, marker damia nut oil, meadow oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil. , animal or plant oils such as candelier wax, carnaba wax, liquid lanolin, and hydrogenated castor oil.

Moisturizers include water-soluble low-molecular-weight moisturizers, oil-soluble molecular moisturizers, water-soluble polymers, and fat-soluble polymers.

Water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (degree of polymerization n = 2 or more), and polypropylene. Examples include glycol (degree of polymerization n = 2 or more), polyglycerin B (degree of polymerization n = 2 or more), lactic acid, lactate, etc.

Examples of fat-soluble low-molecular-weight moisturizers include cholesterol and cholesterol esters.

Water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. You can.

Examples of oil-soluble polymers include polyvinylpyrrolidone and eicosene copolymers, polyvinylpyrrolidone and hexadecene copolymers, nitrocellulose, dextrin fatty acid ester, and polymer silicone. Examples of emollients include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

Examples of surfactants include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE. Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE and POP (polyoxyethylene and polyoxypropylene) copolymer, POE and POP alkyl ether, polyether modified silicone, lauric acid. Examples include alkanolamide, alkylamine oxide, and hydrogenated soybean phospholipid.

Anionic surfactants include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkylnaphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate industry, alkyl amide. Phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. there is.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, and cationic surfactant. Examples include benzalkonium, diethylaminoethylamide stearate, dimethylaminopropyl stearate, and quaternary ammonium salts of lanolin derivatives. Amphoteric surfactants include carboxy beta type, amide beta type, sulfo beta type, hydroxy sulfo beta type, amide sulfo betaine type, phosphobetain type, aminocarboxylate type, imidazoline derivative type, and amidamine type. Amphoteric surfactants, etc. can be mentioned.

Organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium-coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and aluminum oxide. Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene and styrene copolymer, Examples include organic pigments such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments.

Examples of organic powder include metal soap such as calcium stearate; Alkyl phosphate metal salts such as sodium zinc cetilate, zinc laurylate, and calcium laurylate; Acyl amino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amidesulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylizine, N-alpha-paritoylolnithine, N-alpha-lauroylarginine, N-alpha-hydrogenated beef tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Examples include polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene and styrene copolymers, and ethylene tetrafluoride.

UV absorbers include para-aminobenzoic acid, ethyl para-aminobenzoate, amyl para-aminobenzoate, octyl para-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, and benzyl cinnamate. , paramethoxycinnamic acid-2-ethoxyethyl, octyl paramethoxycinnamic acid, mono-2-ethylhexane glyceryl diparamethoxycinnamic acid, isopropyl paramethoxycinnamic acid, diisopropyl and diisopropylcinnamic acid ester mixture, Urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxybenzoate Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Disinfectants include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincphyllithione, benzalkonium chloride, and photosensitive. Sub-No. 301, mononitroguaiacol sodium, undecirenic acid, etc. can be mentioned.

Antioxidants include butylhydroxyanisole, propyl gallate, and elisorbic acid.

Examples of pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, fumal acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, and sodium monohydrogen phosphate.

Examples of alcohol include higher alcohols such as cetyl alcohol.

Additionally, the ingredients that may be added are not limited to this, and any of the above ingredients can be blended within the range that does not impair the purpose and effect of the present invention.

The cosmetic of the present invention may take the form of a solution, emulsion, viscous mixture, etc.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions as active ingredients, for example, conventional auxiliaries and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances. Includes.

The cosmetic composition for preventing hair loss, promoting hair growth, or improving scalp condition of the present invention can be prepared in any formulation commonly manufactured in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, serum, hair Cosmetics, etc. can be mentioned.

Specifically, the cosmetic composition of the present invention includes skin lotion, skin softener, skin toner, milk lotion, astringent, lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, It includes formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, hair lotions, hair tonics, hair essences, hair shampoos, hair rinses, hair treatments, body lotions, and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier ingredient. You can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

In one aspect, the present invention provides a quasi-drug composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

The quasi-drug composition of the present invention refers to products that have a milder effect than pharmaceuticals among products used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases in humans or animals. For example, according to the Pharmaceutical Affairs Act, quasi-drugs are drugs. Excluding articles used for the following purposes, textile and rubber products used for the treatment or prevention of diseases in humans and animals, products that have a minor or no direct effect on the human body, and are not instruments or machines or similar, and products used to prevent infectious diseases. This includes disinfectants, pesticides, etc. The type or formulation of the quasi-drug composition of the present invention is not particularly limited, but is preferably a composition for preventing hair loss, promoting hair growth, or improving scalp condition.

The term "composition for preventing hair loss, promoting hair growth, or improving scalp condition" as used in the present invention refers to a composition that prevents hair loss, promotes hair growth, and prevents or alleviates damage to the scalp and hair. The formulation of the composition for preventing hair loss, promoting hair growth, or improving scalp condition may be shampoo, rinse, essence, nutritional supplement, serum, massage cream, lotion, pack, etc., but is not limited thereto.

The quasi-drug composition of the present invention may further include auxiliaries commonly used in cosmetic compositions to the extent that they do not impair the effect of the present invention. The type of auxiliary that can be used in the present invention is not particularly limited, and any auxiliary commonly used in this technical field can be used. The auxiliaries may include customary auxiliaries such as, for example, gelling agents, activators, preservatives, fillers, antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings.

When the pinitol of the present invention is added to a quasi-drug composition for the purpose of preventing hair loss, promoting hair growth, or improving scalp condition, the pinitol can be added as is or used together with other quasi-drug ingredients, and can be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.

In addition to the above ingredients, the quasi-drug composition of the present invention may further include pharmaceutically acceptable carriers, excipients, or diluents as needed. The pharmaceutically acceptable carrier, excipient, or diluent is not limited as long as it does not impair the effect of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. It can be included.

In one aspect, the present invention provides a health functional food composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient.

In addition to containing the active ingredient pinitol, the food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients like a typical food composition.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose, etc.; Disaccharides such as maltose, sucrose, etc.; and polysaccharides, such as common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The above-described flavoring agents include natural flavoring agents (thaumatin), stevia extracts (e.g. rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.). The food composition of the present invention can be formulated in the same way as the pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gum, candy, ice cream, alcoholic beverages, vitamin complexes, health supplements, etc. There is.

In addition to the extract as an active ingredient, the food composition contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, It may contain alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the food composition of the present invention may contain pulp for the production of natural fruit juice, fruit juice beverages, and vegetable beverages.

The functional food composition of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. In the present invention, “health functional food composition” refers to food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with Act No. 6727 on Health Functional Food, and refers to food that is manufactured and processed with respect to the structure and function of the human body. It means taking it for the purpose of controlling nutrients or obtaining useful health effects such as physiological effects. The health functional food of the present invention may contain common food additives, and its suitability as a food additive is determined in accordance with the general provisions and general test methods of the food additive code approved by the Food and Drug Administration, unless otherwise specified. Judgment is made according to specifications and standards. Items listed in the 'Food Additive Code' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as dark pigment, licorice extract, crystalline cellulose, high-quality pigment, and guar gum; Examples include mixed preparations such as sodium L-glutamate preparations, noodle additive alkaline preparations, preservative preparations, and tar coloring preparations. For example, the health functional food in the form of a tablet is made by granulating a mixture of the active ingredient of the present invention with excipients, binders, disintegrants and other additives in a conventional manner, and then adding a lubricant and compression molding, or The mixture can be directly compression molded. In addition, the health functional food in the form of tablets may contain flavoring agents, etc., if necessary. Among capsule-type health functional foods, hard capsules can be manufactured by filling a regular hard capsule with a mixture of the active ingredient of the present invention mixed with additives such as excipients, and soft capsules can be prepared by mixing the active ingredient of the present invention with additives such as excipients. It can be manufactured by filling the mixture with a capsule base such as gelatin. The soft capsule may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, etc., if necessary. The health functional food in the form of a pill can be prepared by molding a mixture of the active ingredient of the present invention and excipients, binders, disintegrants, etc., using a known method. If necessary, it can be coated with white sugar or other coating agent. Alternatively, the surface can be coated with substances such as starch or talc. Health functional food in the form of granules can be manufactured into granules by mixing a mixture of excipients, binders, disintegrants, etc. of the active ingredients of the present invention by a known method, and may contain flavoring agents, flavoring agents, etc., if necessary. You can.

In one aspect, the present invention provides a pharmaceutical composition for preventing or treating hair loss containing pinitol as an active ingredient.

The pharmaceutical composition of the present invention may further include adjuvants in addition to the active ingredients. Any adjuvant known in the art may be used without limitation, but, for example, Freund's complete adjuvant or incomplete adjuvant may be further included to increase immunity.

The pharmaceutical composition according to the present invention can be prepared by incorporating the active ingredient into a pharmaceutically acceptable carrier. Here, pharmaceutically acceptable carriers include carriers, excipients, and diluents commonly used in the pharmaceutical field. Pharmaceutically acceptable carriers that can be used in the pharmaceutical composition of the present invention include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, Examples include calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical composition of the present invention can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions according to conventional methods. .

When formulated, it can be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations contain the active ingredient plus at least one excipient, such as starch, calcium carbonate, sucrose, lactose, and gelatin. It can be prepared by mixing etc. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc can also be used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups. In addition to the commonly used diluents such as water and liquid paraffin, they contain various excipients such as wetting agents, sweeteners, fragrances, and preservatives. You can. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, tween 61, cacao, laurel, glycerogelatin, etc. can be used.

The pharmaceutical composition according to the present invention can be administered to an individual through various routes. All modes of administration are contemplated, for example, by oral, intravenous, intramuscular, subcutaneous, or intraperitoneal injection.

The dosage of the pharmaceutical composition according to the present invention is selected taking into account the age, weight, gender, physical condition, etc. of the individual. It is obvious that the concentration of the active ingredient included in the pharmaceutical composition can be selected in various ways depending on the target, and is preferably included in the pharmaceutical composition at a concentration of 0.01 to 5,000 μg/ml. If the concentration is less than 0.01 ㎍/ml, pharmaceutical activity may not appear, and if it exceeds 5,000 ㎍/ml, it may be toxic to the human body.

The pharmaceutical composition may be formulated into various oral or parenteral dosage forms.

Dosage forms for oral administration include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, granules, etc. These dosage forms contain diluents (e.g. lactose, dextrose, water) in addition to the active ingredient. crose, mannitol, sorbitol, cellulose and/or glycine), lubricants (e.g. silica, talc, stearic acid and its magnesium or calcium salts and/or polyethylene glycol). Additionally, the tablets may contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and/or polyvinylpyrrolidine, and in some cases starch, agar, alginic acid. or disintegrants such as sodium salts thereof or effervescent mixtures and/or absorbents, colorants, flavoring and sweetening agents. The formulation can be prepared by conventional mixing, granulating or coating methods.

In addition, representative formulations for parenteral administration are injectable formulations, and solvents for injectable formulations include water, Ringer's solution, isotonic saline solution, or suspension. The sterile fixed oil of the injectable preparation can be used as a solvent or suspending medium, and any non-irritating fixed oil, including mono- and di-glycerides, can be used for this purpose.

Additionally, the injectable preparation may use fatty acids such as oleic acid.

Hereinafter, the present invention will be described in more detail through examples. However, the above examples and experimental examples are presented as examples of the present invention, and if a detailed description of a technique or configuration well known to those skilled in the art is judged to unnecessarily obscure the gist of the present invention, the detailed description is omitted. It can be done, and the present invention is not limited thereby. The present invention is capable of various modifications and applications within the description of the claims described below and the scope of equivalents interpreted therefrom.

<Example 1> Effect of D-pinitol on improving wound healing of dermal papilla cells

In order to confirm the effect of pinitol of the present invention on preventing hair loss, promoting hair growth, or improving scalp condition, an experiment was conducted on the effect of D-pinitol on improving wound healing in human scalp-derived dermal papilla cells, which are a hair loss cell model.

Human dermal papilla cells, which are key cells in preventing human hair loss, were cultured at 5 × 10 ⁴ /ml in a 35 mm dish cell culture dish, then scratched with the end of the yellow tip in the center, and then treated with H as a negative control. ₂ After treating the O ₂ (375 μM) treatment group and D-pinitol (10, 50, and 100 μM) for 24 hours, the cell number and shape of human dermal papilla cells were observed using a microscope (Phase contrast microscopy), and the resulting image is is shown in Figure 1.

As a result, as can be seen in Figure 1, compared to the negative control group treated with H ₂ O ₂ (375 μM), the number of human tumor cells treated with D-pinitol increased, resulting in significant recovery of the central region. (Each data is a representative image, n=3).

<Example 2> Effect of D-pinitol on increasing Alkaline Phosphatase expression in dermal papilla cells

In order to confirm the effect of pinitol of the present invention on preventing hair loss, promoting hair growth, or improving scalp condition, an experiment was conducted on the effect of D-pinitol on increasing the expression of alkaline phosphatase, a key biomarker in human scalp-derived dermal papilla cells, which are a hair loss cell model. did.

Human dermal papilla cells, which are key cells in preventing human hair loss, were cultured at 5 × 10 ⁴ /ml in a 24-well cell culture dish, and then treated with H ₂ O ₂ (375 μM) as a negative control group and a positive control group. After treatment with phosphorus minoxidil (10 nM) and D-pinitol (10, 50 μM) for 24 hours, the culture medium was removed and the fixation solution was treated for 5 minutes. Meanwhile, in negative control group 1, human dermal papilla cells were treated with a fixing solution for 5 minutes.

After removing the fixing solution from each group, each group was treated with alkaline phosphatase substrate (Abcam) for 30 minutes, the stained cells were washed with distilled water, and analyzed under a microscope (Phase contrast). Cells stained blue were observed using microscopy, and the resulting image is shown in Figure 2.

As a result, as can be seen in Figure 2, compared to the negative control group treated with H ₂ O ₂ (375 μM), in the human tumor cells treated with D-pinitol (10 and 50 μM), blue cells were observed. The number of cells was significantly increased, and an increase in alkaline phosphatase was observed (each data is a representative image, n=3).

<Example 3> Reactive oxygen species (ROS) inhibition effect of D-pinitiol on dermal papilla cells

In order to confirm the effectiveness of pinitol of the present invention in preventing hair loss, promoting hair growth, or improving scalp condition, an experiment was conducted on the inhibitory effect of D-pinitiol on human scalp-derived dermal papilla cells, which are a hair loss cell model.

Human dermal papilla cells, which are key cells in preventing human hair loss, were cultured in a 96 well cell culture dish at ^2.5 After pre-treatment with H ₂ O ₂ (375 μM) for 24 hours, reactive oxygen species were induced for 1 hour. DCF (2', 7' -dichlorofluorescein)-DA ROS assay was performed as follows (Abcam, Cambridge, UK). Remove the cell culture medium, wash out the 1X buffer per well, add 100 μl of DCF-DA solution (20 μM) to each well, cover with aluminum foil to block light, and incubate in a cell incubator at 37 degrees for 45 minutes. During incubation. After the reaction was completed, the DCF-DA solution was removed, washed out with 1X buffer, and Excitation/Emission was measured at 485/535 nm using a microplate reader (Biotek, CA, USA).

As a result, as can be seen in Figure 3, the value of reactive oxygen species (ROS) increased by H ₂ O ₂ (375 μM) was reduced by treatment with D-pinitol (10, 50 μM). was confirmed (n=5).

Therefore, through the above examples, it was confirmed that pinitol of the present invention can be used as a composition for preventing hair loss, promoting hair growth, or improving scalp condition, thereby completing the present invention.

As seen above, the present invention has been described as the preferred embodiment mentioned above, but those skilled in the art will find that other regressive inventions can be found by adding, changing, deleting, etc. other components within the same technical scope. However, other embodiments that are included within the scope of the present invention can be easily proposed. Therefore, the embodiments described above are illustrative in all respects and do not limit the scope of the present invention.

Claims (8)

A cosmetic composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient. According to paragraph 1,
A composition wherein the pinitol is represented by Formula 1:
[Formula 1]
. According to paragraph 1,
The composition has an effect of improving wound healing of dermal papilla cells. According to paragraph 1,
The composition increases the expression of alkaline phosphatase in dermal papilla cells. According to paragraph 1,
The composition has an effect of suppressing free radicals in dermal papilla cells. A quasi-drug composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient. A health functional food composition for preventing hair loss, promoting hair growth, or improving scalp condition, containing pinitol as an active ingredient. A pharmaceutical composition for preventing or treating hair loss containing pinitol as an active ingredient.