CN103125518A - Controlling agrochemical for kiwifruit canker and controlling method thereof - Google Patents
Controlling agrochemical for kiwifruit canker and controlling method thereof Download PDFInfo
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- CN103125518A CN103125518A CN2013100980344A CN201310098034A CN103125518A CN 103125518 A CN103125518 A CN 103125518A CN 2013100980344 A CN2013100980344 A CN 2013100980344A CN 201310098034 A CN201310098034 A CN 201310098034A CN 103125518 A CN103125518 A CN 103125518A
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Abstract
The invention relates to a controlling agrochemical for kiwifruit canker and a controlling method thereof, and belongs to the protective technical field of agricultural plants. The controlling agrochemical for kiwifruit canker aims at solving the problems that the kiwifruit canker has the characteristics of being disguised, fulminic and destructive, and resistance is generated by using the single medicine for a long time. Pathogenic bacterium target is confirmed by adopting the molecular biological technique; a plurality of commodity drugs which are effective to a plurality of targets are screened indoors by adopting an inhibition zone method to find six efficient medicines; the efficient medicines are prepared into drugs for being applied to the fields; and the kiwifruit orchard canker caused by the rainy weather in spring is prevented and controlled well by spraying and coating the controlling agrochemical.
Description
Technical field
This research relates to the agriculture plant protection field, specifically is used for prevention and control medicament and the preventing control method of Prospect on Kiwifruit Bacterial Canker.
Background technology
By the pseudomonas syringae kiwi fruit cause a disease mutation (
Pseudomonas syringae pv.Actinidae, the Prospect on Kiwifruit Bacterial Canker that PSA) causes is the disease of a kind of serious threat kiwi fruit plant husbandry, the main limiting factor that threatens especially the kiwi fruit industry development.This disease has disguise, burst, destructive characteristics, and the present situation of the generation resistance of long-time single drug.Therefore, the prevention and control of this disease must be comprised of reasonably efficient medicament and method.At first the present invention confirms the pathogenic bacteria target by Protocols in Molecular Biology, and, Vivo Studies on Screening stripped through efficient medicament in conjunction with preventive measure, reach effective prevention and control ulcer disease purpose.
Summary of the invention
The object of the invention is at first confirm the pathogenic bacteria target by Protocols in Molecular Biology, pass through inhibition zone method, with multiple, the effective commodity medicament of bacteriosis is carried out lab screening, filter out highly effective pesticide and the highly effective pesticide that filters out is mixed with medicament, effectively be applied in the anti-control techniques of Prospect on Kiwifruit Bacterial Canker.
At first the present invention carries out bacterium colony PCR by selectivity cultivation, separation, purifying, use 16s rRNA, obtain 16s r RNA sequence, by comparing at NCBI gene pool Blast, obtain high homology sequence, draw the object bacteria kind according to the high homology sequence of searching for and belong to the pseudomonas syringae genus, in conjunction with the Physiology and biochemistry experiment of this bacterial classification, further confirm, thereby obtain target.Pass through inhibition zone method, with multiple, the effective commodity medicament of bacteriosis is carried out lab screening, find 6 kinds of efficient medicaments, it is followed successively by Prospect on Kiwifruit Bacterial Canker bacterium inhibitory action size: 20% bronopol＞72% streptomycin sulphate＞64% is disliked white MnZn＞75% tpn＞80% mancozeb＞30% S-Ethyl ethylthio sulfonate, and finds that 70% thiophanate-methyl, 45% fenaminosulf, 20% copper acetate, 20% Yekuzuo, 20% Thiodiazole-copper, 30% tiger glue adipic acid copper, 2% kasugarnycin, 20% Fluoxastrobin and 3% fenaminosulf are all without bacteriostasis.6 kinds of efficient medicament inhibitions see Table 1.Then the efficient medicine of gained is made medicament and be applied to the field, and continue rainy characteristics in conjunction with spring in Guizhou, use the efficient medicine of screening gained to mix with vaseline, lard and the plant tissue promoter (one or both in methyl α-naphthyl acetate, heteroauxin) of resistance of rainwater washing against scab is struck off, smears.
The indoor inhibition of 6 kinds of medicaments of table 1 to the Prospect on Kiwifruit Bacterial Canker bacterium
The present invention is used for the prevention and control medicament of Prospect on Kiwifruit Bacterial Canker, its prevention and control medicament is: the liniment that medicament active ingredient and callus promoter and substrate combination form, or be that the spraying agent of medicament active ingredient preparation, its Chinese medicine active ingredient refer to that 20% bronopol, 75% agricultural streptomycin, 64% dislike the mixture of any one or any two kinds in white MnZn, 75% tpn, 80% mancozeb or 30% S-Ethyl ethylthio sulfonate; Callus promoter refers to one or both the mixture in methyl α-naphthyl acetate, heteroauxin, matrix is one or both mixtures in lard, vaseline, and liniment quality proportioning is: medicament active ingredient: callus promoter: matrix=1:0.5:98.5.Mix in proportion and get final product, spray agent concentration is 800 ~ 1200 times of medicament active ingredient dilutions.In any two kinds of mixtures of medicament active ingredient, 20% bronopol, 75% agricultural streptomycin, the 80% shared mass ratio of mancozeb are that to dislike the shared mass ratio of white MnZn be 50% ~ 30% for 50 ~ 70%, 30% S-Ethyl ethylthio sulfonate, 75% tpn, 64%; In the callus accelerator mixture, methyl α-naphthyl acetate and heteroauxin mass ratio are 1:1; In substrate mixture, lard and vaseline mass ratio are 7:3.
The present invention is used for the preventing control method of Prospect on Kiwifruit Bacterial Canker, be after the kiwi fruit fruit picking, beta pruning, clear garden and after the beginning of spring, every 15 days, one or both the composite mixtures in medicament active ingredient are sprayed, until temperature rises to more than 20 ℃ to kiwi fruit plant and orchard; After canker occurs, with lesion scraping, with liniment, focus is smeared, smear once weekly, until till focus stops flowing out red liquid.
Said method adopts respectively 6 kinds of agricultural chemicals alternately spray or alternately spray or replace sprinkling with one or more mixtures in 6 kinds of agricultural chemicals with two kinds of mixtures in 6 kinds of agricultural chemicals when being to make the made spray agent of with medicament active ingredient.
Said method is when using liniment, and one adopts the liniment of single medicament active ingredient and single callus and single substrate preparation to be used alternatingly; Its two, also or adopt the liniment of any two kinds of medicament active ingredients and two kinds of callus and two kinds of substrate preparations to be used alternatingly; Its three, also or with one and its two be used alternatingly.
Such scheme is implemented at Xiuwen County, Guizhou Province Chinese gooseberry garden year February in October, 2010 to 2013, and the prevention and control area is 10 mu.The prevention and control data showed in 2011 ~ 2012 years, and what canker occured for whole prevention and control districts only has 24 strains, 33.2% dropped to 8.67% after control before prevent and treat; Canker wound sum drops to 56 places from 212 of spring in 2011, illustrates that control efficiency is good.After using liniment, the Wound healing rate reaches more than 97%.The use of also finding compound drug in research more only uses the prevention and control districts effect of agricultural streptomycin to want obviously, and the incidence of disease is lower than 13%.
Embodiment
Implement to set respectively Three regions (contrast, prevention and control 1st district, prevention and control 2nd district) and carry out dispenser.
Embodiment one (prevention and control 1st district)
Spraying compound drug is:
1. 7:3 is composite in mass ratio for 20% bronopol and 30% S-Ethyl ethylthio sulfonate, 1000 times of liquid;
2. 5:5 is composite in mass ratio for 72% streptomycin sulphate and 75% tpn, 1500 times of liquid;
3. 7:3 is composite in mass ratio for 80% mancozeb and the 64% white MnZn of evil, 800 times of liquid;
Daubing medicament is:
Antipathogenic composition: 20% bronopol and 75% tpn 7:3 in mass ratio mix;
Callus promoter: methyl α-naphthyl acetate and heteroauxin 1:1 in mass ratio mix;
Matrix: lard and vaseline 7:3 in mass ratio mix;
Antipathogenic composition, callus promoter, matrix are mixed in mass ratio at 1: 0.5: 98.5.
Concrete using method is:
Use compound drug 1. the fruit tree complete stool to be sprayed after fruit picking; In the orchard the complete rear use compound drug of beta pruning is 2. to spraying in plant and orchard comprehensively, and to plant below 1.2 meters and larger clip whitewash processing, clear garden.After temperature rises to 15 ℃ of left and right, use respectively three kinds of compound drugs at interval of 15 days, plant to be sprayed.
Find that on March 7th, 2012 canker occurs, first use scraper with the affected part scraping, use liniment that affected part is smeared, weekly, occur until no longer see red liquid.
By above-mentioned measure, in the prevention and control districts, the canker incidence of disease is 6.11%, and the check plot is 33.2%; The canker wound is 47 places, and the check plot is 212 places.After using liniment, the Wound healing rate reaches 97%.
Embodiment two (prevention and control 2nd district)
Spraying medicament is:
1. 5:5 is composite in mass ratio for 20% bronopol and 75% tpn, 800 times of liquid;
2. 7:3 is composite in mass ratio for 72% streptomycin sulphate and 30% S-Ethyl ethylthio sulfonate, 1200 times of liquid;
3. 7:3 is composite in mass ratio for 80% mancozeb and the 64% white MnZn of evil, 800 times of liquid;
Daubing medicament is:
Antipathogenic composition: 72% streptomycin sulphate and 20% bronopol 5:5 in mass ratio mix;
Callus promoter: methyl α-naphthyl acetate and heteroauxin 1:1 in mass ratio mix;
Matrix: lard and vaseline 7:3 in mass ratio mix;
Antipathogenic composition, callus promoter, matrix are mixed in mass ratio at 1: 0.5: 98.5.
Concrete using method is:
Use after fruit picking and spray medicament (1) the fruit tree complete stool is sprayed; In the orchard, the complete rear use of beta pruning sprays medicament (2) to spraying in plant and orchard comprehensively, and to plant below 1.2 meters and larger clip whitewash processing, clear garden.After temperature rises to 15 ℃ of left and right, use respectively three kinds of compound drugs at interval of rotation in 15 days, plant to be sprayed.
Find that on March 19th, 2012 canker occurs, first use scraper with the affected part scraping, use liniment that affected part is smeared, every 7 days once, occurs until no longer see red liquid.
By above-mentioned measure, in the prevention and control districts, the canker incidence of disease is 5.37%, and the check plot is 33.2%; The canker wound is 43 places, and the check plot is 212 places.After using liniment, the Wound healing rate reaches more than 97%.
Claims (4)
1. be used for the prevention and control medicament of Prospect on Kiwifruit Bacterial Canker, it is characterized in that the prevention and control medicament is: the liniment that medicament active ingredient and callus promoter and substrate combination form, or be that the spraying agent of medicament active ingredient preparation, its Chinese medicine active ingredient refer to that 20% bronopol, 75% agricultural streptomycin, 64% dislike the mixture of any one or any two kinds in white MnZn, 75% tpn, 80% mancozeb or 30% S-Ethyl ethylthio sulfonate; Callus promoter refers to one or both the mixture in methyl α-naphthyl acetate, heteroauxin, matrix is one or both mixtures in lard, vaseline, liniment quality proportioning is: medicament active ingredient: callus promoter: matrix=1:0.5:98.5, mix in proportion and get final product, spray agent concentration is 800～1500 times of medicament active ingredient dilutions; In any two kinds of mixtures of medicament active ingredient, 20% bronopol, 75% agricultural streptomycin, the 80% shared mass ratio of mancozeb are that to dislike the shared mass ratio of white MnZn be 50%～30% for 50～70%, 30% S-Ethyl ethylthio sulfonate, 75% tpn, 64%; In the callus accelerator mixture, methyl α-naphthyl acetate and heteroauxin mass ratio are 1:1; In substrate mixture, lard and vaseline mass ratio are 7:3.
2. according to the preventing control method for Prospect on Kiwifruit Bacterial Canker claimed in claim 1, it is characterized in that after the kiwi fruit fruit picking, beta pruning, clear garden and after the beginning of spring, every 15 days, one or both composite mixtures in medicament active ingredient are sprayed, until temperature rises to more than 20 ℃ to kiwi fruit plant and orchard; After canker occurs, with lesion scraping, with liniment, focus is smeared, smear once weekly, until till focus stops flowing out red liquid.
3. the preventing control method for Prospect on Kiwifruit Bacterial Canker according to claim 2 adopts respectively 6 kinds of agricultural chemicals alternately spray or alternately spray or replace sprinkling with one or more mixtures in 6 kinds of agricultural chemicals with two kinds of mixtures in 6 kinds of agricultural chemicals when it is characterized in that making the made spray agent of with medicament active ingredient.
4. the preventing control method for Prospect on Kiwifruit Bacterial Canker according to claim 2 is characterized in that when using liniment, and one adopts the liniment of single medicament active ingredient and single callus and single substrate preparation to be used alternatingly; Its two, also or adopt the liniment of any two kinds of medicament active ingredients and two kinds of callus and two kinds of substrate preparations to be used alternatingly; Its three, also or with one and its two be used alternatingly.
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604609A (en) * | 2015-02-10 | 2015-05-13 | 贵州三福生物科技有限公司 | Method for controlling and eliminating kiwifruit cankers |
| CN104628481A (en) * | 2015-02-10 | 2015-05-20 | 贵州三福生物科技有限公司 | Fertilizer and pesticide combination reagent for preventing and killing bacterial canker of kivifruit and preparation method of fertilizer and pesticide combination |
| CN104782423A (en) * | 2015-05-13 | 2015-07-22 | 西安拓达农业科技有限公司 | Three-dimensional prevention and treatment method of bacterial canker of kiwifruits |
| CN104782436A (en) * | 2015-03-30 | 2015-07-22 | 牟定县大丰农业开发有限责任公司 | Red-heart kiwi fruit canker control method |
| CN104982262A (en) * | 2015-06-15 | 2015-10-21 | 重庆市万州区科技信息中心 | Method for prevention and control of kiwifruit canker disease |
| CN105010432A (en) * | 2015-07-17 | 2015-11-04 | 广西南宁碧湾园林工程有限公司 | Camellia canker treatment drug and treatment method thereof |
| CN105379589A (en) * | 2015-10-23 | 2016-03-09 | 四川禾丰源农资连锁有限公司 | Method for preventing and treating kiwifruit canker |
| CN106212506A (en) * | 2016-07-29 | 2016-12-14 | 赵冰丽 | A kind of prevention and controls of paulownia Peptic Ulcers |
| CN107347335A (en) * | 2017-06-09 | 2017-11-17 | 李永权 | A kind of method based on transfusion preventing and treating Kiwi berry fruit tree canker |
| CN107926405A (en) * | 2017-11-16 | 2018-04-20 | 贵阳学院 | A kind of Synthetical prevention method of Kiwi berry soft rot |
| CN108124723A (en) * | 2018-01-27 | 2018-06-08 | 苏州艾兰绿化工程有限公司 | Improve the soil ball size matching process of transplanting trees surviving rate |
| CN111202082A (en) * | 2020-01-17 | 2020-05-29 | 中国科学院武汉植物园 | Prevention and control agent for kiwifruit canker and method thereof |
| CN111226997A (en) * | 2020-01-17 | 2020-06-05 | 广西壮族自治区农业科学院花卉研究所 | Medicine for preventing and treating kiwifruit canker and using method thereof |
| CN111345318A (en) * | 2020-03-30 | 2020-06-30 | 陕西齐峰农资电商科技有限公司 | Kiwi fruit ulcer disease preventing sealing paint and synthesis process and use method thereof |
| CN114916357A (en) * | 2022-05-16 | 2022-08-19 | 十堰市农业科学院 | Integrated method of field green comprehensive prevention and control technology for kiwifruit canker |
| CN115281186A (en) * | 2022-08-06 | 2022-11-04 | 贵州大学 | Smearing preparation for preventing and treating kiwifruit canker and preparation method thereof |
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2013
- 2013-03-26 CN CN2013100980344A patent/CN103125518A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604609B (en) * | 2015-02-10 | 2017-05-31 | 贵州三福生物科技有限公司 | A kind of method for preventing and kill off Prospect on Kiwifruit Bacterial Canker |
| CN104628481A (en) * | 2015-02-10 | 2015-05-20 | 贵州三福生物科技有限公司 | Fertilizer and pesticide combination reagent for preventing and killing bacterial canker of kivifruit and preparation method of fertilizer and pesticide combination |
| CN104604609A (en) * | 2015-02-10 | 2015-05-13 | 贵州三福生物科技有限公司 | Method for controlling and eliminating kiwifruit cankers |
| CN104782436A (en) * | 2015-03-30 | 2015-07-22 | 牟定县大丰农业开发有限责任公司 | Red-heart kiwi fruit canker control method |
| CN104782423A (en) * | 2015-05-13 | 2015-07-22 | 西安拓达农业科技有限公司 | Three-dimensional prevention and treatment method of bacterial canker of kiwifruits |
| CN104982262A (en) * | 2015-06-15 | 2015-10-21 | 重庆市万州区科技信息中心 | Method for prevention and control of kiwifruit canker disease |
| CN105010432A (en) * | 2015-07-17 | 2015-11-04 | 广西南宁碧湾园林工程有限公司 | Camellia canker treatment drug and treatment method thereof |
| CN105010432B (en) * | 2015-07-17 | 2017-08-25 | 广西南宁碧湾园林工程有限公司 | The medicine and its treatment method of camellia canker |
| CN105379589A (en) * | 2015-10-23 | 2016-03-09 | 四川禾丰源农资连锁有限公司 | Method for preventing and treating kiwifruit canker |
| CN106212506A (en) * | 2016-07-29 | 2016-12-14 | 赵冰丽 | A kind of prevention and controls of paulownia Peptic Ulcers |
| CN107347335A (en) * | 2017-06-09 | 2017-11-17 | 李永权 | A kind of method based on transfusion preventing and treating Kiwi berry fruit tree canker |
| CN107926405A (en) * | 2017-11-16 | 2018-04-20 | 贵阳学院 | A kind of Synthetical prevention method of Kiwi berry soft rot |
| CN108124723A (en) * | 2018-01-27 | 2018-06-08 | 苏州艾兰绿化工程有限公司 | Improve the soil ball size matching process of transplanting trees surviving rate |
| CN111202082A (en) * | 2020-01-17 | 2020-05-29 | 中国科学院武汉植物园 | Prevention and control agent for kiwifruit canker and method thereof |
| CN111226997A (en) * | 2020-01-17 | 2020-06-05 | 广西壮族自治区农业科学院花卉研究所 | Medicine for preventing and treating kiwifruit canker and using method thereof |
| CN111345318A (en) * | 2020-03-30 | 2020-06-30 | 陕西齐峰农资电商科技有限公司 | Kiwi fruit ulcer disease preventing sealing paint and synthesis process and use method thereof |
| CN114916357A (en) * | 2022-05-16 | 2022-08-19 | 十堰市农业科学院 | Integrated method of field green comprehensive prevention and control technology for kiwifruit canker |
| CN115281186A (en) * | 2022-08-06 | 2022-11-04 | 贵州大学 | Smearing preparation for preventing and treating kiwifruit canker and preparation method thereof |
| CN115281186B (en) * | 2022-08-06 | 2023-06-27 | 贵州大学 | Smearing preparation for preventing and treating kiwi fruit canker and preparation method thereof |
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Application publication date: 20130605 |