CN103083459B - Traditional Chinese medicinal compound preparation for treating constipation, and preparation method and application thereof - Google Patents
Traditional Chinese medicinal compound preparation for treating constipation, and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicinal compound preparation for treating constipation. The preparation is prepared through using the following raw materials, by weight, 5-10 parts of ochre, 10-15 parts of Chinese angelica, 3-10 parts of sodium sulfate powder, 15-20 parts of prepared rehmannia root, 15-25 parts of Rehmannia glutinosa Libosch, 3-10 parts of Rheum officinale, 15-25 parts of Fructus Cannabis, 5-10 parts of safflower, 5-10 parts of Peach Seed, 1-10 parts of Rhizoma Cimicifugae and 1-10 parts of licorice. In the above formula, ochre and Chinese angelica which have the efficacies of blood generating and nourishing, dryness moisturizing and intestine lubricating, and the prepared rehmannia root and the sodium sulfate powder which have the efficacies of blood nourishing, yin nourishing, heat clearing and fire purging are used as monarch medicines; Rehmannia glutinosa Libosch having yin nourishing and body liquid engendering efficacies, the Fructus Cannabis having intestine lubricating and bowel relaxing efficacies, and the Rheum officinale having a purgation efficacy are used as minister medicines to assist the ochre, Chinese angelica, the prepared rehmannia root and the sodium sulfate powder to realize the dryness moisturizing, intestine lubricating, hardness softening and bowel relaxing; the Peach Seed and the safflower which have the blood circulation invigorating and blood nourishing efficacies are used as assistant medicines to assist the ochre and the Chinese angelica to realize blood generating and nourishing; and Rhizoma Cimicifugae has the yang invigorating and spleen helping efficacies, and introduces all other medicines to the spleen and lung channels, and licorice coordinates the medicine actions. The traditional Chinese medicinal compound preparation for treating constipation prepared in the invention has the advantages of simple technology control, high biological availability, good medicine effect, fast curative effect and low cost.
Description
Technical field
The present invention relates to field of traditional Chinese medicine pharmacy, be specifically related to a kind of compound Chinese medicinal preparation for the treatment of constipation and its preparation method and application.
Background technology
Theory of Chinese medical science thinks that constipation is that constipation is obstructed, and defecation time extends or wish is defecated and involved and abstruse not smooth a kind of disease.As far back as Han dynasty, doctor just proposes FU QI being obstructed and causes and decline and the opinion of constipation.Ancient Chinese medicine doctor thinks that constipation is all relevant with kidney, spleen, stomach, large intestine, lung, qi-blood-body fluid, cold and heat and asthenia and sthenia etc.Not normal and the QI failing to descend of becoming thoroughly decomposed of stomach, transporting mistake department and the clear QI failing to ascend of spleen, small intestinal secrete not Shi Zhi, the conduction abnormalities of large intestine; The main liquid of kidney, lung governing qi, also can cause constipation, and the disease of dryness accumulated in the stomach and intestine differs in the time suffering from a deficiency of the kidney the dryness of the lung, has dry, the wind-dryness of heat, constipation caused by accumulation of heat and constipation of YIN type, has again the old deficiency of vital energy, deficiency of body fluid and dryness accumulated in the stomach and intestine person; Also about heat and the cold discussion that can cause constipation: " constipation person, is got along well by three warmers the five internal organs, and cold and hot gas is uncomfortable, and steam enters the intestines and stomach partially, and body fluid exhausts dry, therefore make the no knot of waste matter, obstruction also." doctor of the Qing Dynasty proposed the constipation viewpoint relevant with the dryness of the lung: " and stool closes knot, and people thinks large dryness of the intestine very, and who knows it is that lung qi is dry? clear respectful gas can not be descending in large intestine for the dryness of the lung." more have doctor to propose all relevant discussions such as constipation and sexual life, diet, seven emotions etc.Aspect treatment, motherland medical science propose " as absurd with high sharp medicine by it, body fluid is walked, QI and blood consumption, though logical and be secret temporarily." except therapeutic scheme, also utilize guiding art.
Along with social senilization, the change of modern life rhythm and dietary habit etc., constipation more and more comes into one's own.Constipation is not only affects one of important benign disease of modern's quality of life, and close with colorectal cancer onset relation, and fatality rate is higher indirectly.But due to the difference of the factors such as race, bowl evacuation habit, dietary structure, environment, widely different to the concept of constipation and diagnosis, its accurate sickness rate cannot be added up.In the U.S., just exceed 2,500,000, the patient who more takes aperient in addition with the constipation medical patient that is chief complaint.The use self-appraisal way of questionnaires such as domestic Dai Fei are investigated 1535 healthy populations, and chronic constipation crowd's total prevalence rate is 9.18%, and male is 7.28%, and women is that more than 11.04%, 60 years old person is 14.84%.Prolonged constipation harm has four: when the stiff stool of one is discharged, scratch intestinal mucosa, causes having blood in stool and anal fissure; Its two hard stool is piled up at internal rectum, and oppresses the blood vessel on rectal wall, makes the venous return generation obstacle of rectal wall, and the vein at this place will tortuously be expanded, and forms internal hemorrhoid or external hemorrhoid; Its three constipation with dry stool and defecation are not smooth, and patient often has some subjective symptomss, following abdominal distention, and just sense or aerofluxus do not increase etc. only, and affect digestive function, and therefore appetite also go down; Its four for having a heart disease, the patient of cerebrovascular, can bring out heart failure or rupture of blood vessel in brain is hemorrhage, when serious, can jeopardize patients ' lives.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, compound Chinese medicinal preparation of the treatment constipation of provide a kind of and act on steadily, low price, curative effect are definite and preparation method thereof.
To achieve these goals, technical scheme provided by the invention is: a kind of compound Chinese medicinal preparation for the treatment of constipation, described preparation is made up of the raw material of following weight proportion: Haematitum 5-10 part, Radix Angelicae Sinensis 10-15 part, Matrii Sulfas Exsiccatus 3-10 part, Radix Rehmanniae Preparata 15-20 part, Radix Rehmanniae 15-25 part, Radix Et Rhizoma Rhei 3-10 part, Fructus Cannabis 15-25 part, Flos Carthami 5-10 part, Semen Persicae 5-10 part, Rhizoma Cimicifugae 1-10 part, Radix Glycyrrhizae 1-10 part.
Further, above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, preferably, described preparation is made up of the raw material of following weight proportion: 9 parts of Haematitums, 15 parts of Radix Angelicae Sinensis, 4.5 parts of Matrii Sulfas Exsiccatus, 15 parts of Radix Rehmanniae Preparata, 20 parts of Radix Rehmanniae, 4.5 parts of Radix Et Rhizoma Rhei, 20 parts of Fructus Cannabiss, 6 parts, Flos Carthami, 9 parts, Semen Persicae, 3 parts of Rhizoma Cimicifugaes, 3 parts, Radix Glycyrrhizae.
Further, above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, described preparation is granule.
Second object of the present invention has been to provide the preparation method of the compound Chinese medicinal preparation of above-mentioned treatment constipation, comprises the following steps:
1) take Radix Angelicae Sinensis, the Flos Carthami in raw material according to above-mentioned proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adding quality is powder 2-10 water soaking 0.5-2 hour doubly after sieving, adopt vapor distillation 5-15 hour, collect volatile oil by volatile oil extractor, the remaining medicinal liquid of isolated by filtration and medicinal residues, obtain medicinal liquid one;
2) take Haematitum, Radix Rehmanniae Preparata, Radix Rehmanniae, Rhizoma Cimicifugae and the Radix Glycyrrhizae in raw material according to the above-mentioned proportional quantity of power, after pulverizing, crossing aperture is 20 object sieves, add the medicinal residues that obtain in step 1), add water and reflux 3 times, the water at every turn adding is 3-10 times of medicinal residues quality, each backflow 0.5-2 hour, reflux temperature is 100
oc, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues, obtain medicinal liquid two;
3) medicinal liquid one and the step 2 that step 1) are obtained) medicinal liquid two merging that obtain, with quality percentage composition be 60-80% ethanol precipitation 8-10 hour, get clear liquid medicine, obtain medicinal liquid three;
4) take raw material Radix Et Rhizoma Rhei, Semen Persicae and Fructus Cannabis according to above-mentioned proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adding quality percentage composition is 50-80% alcohol reflux 3 times, add at every turn ethanol quality be medicinal powder 2-10 doubly, each backflow 0.3-2 hour, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues, obtain medicinal liquid four;
5) medicinal liquid four merging that the medicinal liquid three step 3) being obtained and step 4) obtain, are condensed into the extractum that relative density is 1.28-1.36, then 50 DEG C of vacuum dryings 10 hours, pulverize and obtain extract powder;
6) in volatile oil step 1) being obtained, adding quality is that the cyclodextrin of 6 times of volatile oil carries out clathration, and enclose temperature is 60 DEG C, and enclose makes volatile oil clathrate compound for 1 hour;
7) take raw material Matrii Sulfas Exsiccatus according to the proportional quantity described in claim 1 or 2, after pulverizing, crossing aperture is 20 object sieves, after the extract powder obtaining with step (5), the volatile oil clathrate compound that step 6) obtains mix, add the soluble starch of 0.5 times of mixed-powder quality, then make soft material after adding adhesive;
8) it is that 14 object sieve series become wet granular that soft material step 7) being obtained is crossed aperture, and wet granular is dried after 1 hour with 20 mesh sieve granulate, subpackage at 40-45 DEG C, obtains granule.
Further, the preparation method of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, in described step 1), is that to add quality be the water soaking 1 hour of 8 times, powder after sieving, and adopts vapor distillation 10 hours.
Further, the preparation method of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, described step 2) add water and reflux in 3 processes, the water adding for 3 times is respectively 8 times, 6 times and 6 times of medicinal residues quality, refluxes 1 hour at every turn.
Further, the preparation method of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, in described step 3), is the ethanol precipitation that is 70% with quality percentage composition 9 hours; In described step 4), be that to add quality percentage composition be 60% alcohol reflux 3 times, add for 3 times the quality of 60% ethanol to be respectively 7 times, 6 times and 6 times of medicinal residues quality, 20min refluxes at every turn.
Further, the preparation method of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, the cyclodextrin in described step 6) is beta-schardinger dextrin-.
Further, the preparation method of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, the adhesive in described step 7) is 80% ethanol.
The 3rd object of the present invention has been to provide the application of above-mentioned compound Chinese medicinal preparation in the medicine of preparation treatment constipation.
Raw material of Chinese medicine in Chinese medicine preparation of the present invention, especially adjuvant drug and make medicine can with suitably to have the identical property of medicine Chinese medicine replace, its drug effect of the Chinese medicine preparation after replacement is constant.
Beneficial effect of the present invention is: a kind of compound Chinese medicinal preparation for the treatment of constipation provided by the invention, and wherein, taking Haematitum, Radix Angelicae Sinensis as passage, nourishing blood, moisturize laxation, Radix Rehmanniae Preparata, Matrii Sulfas Exsiccatus are 4 taste monarchs drug of nourishing the blood and yin, clearing away heat-fire; Taking Radix Rehmanniae as YIN nourishing and the production of body fluid promoting, Fructus Cannabis is the auxiliary Haematitum of loosening bowel to relieve constipation, Radix Et Rhizoma Rhei 3 taste ministerial drugs under rushing down, Radix Angelicae Sinensis; With Radix Rehmanniae Preparata, Matrii Sulfas Exsiccatus for moisturizing laxation, softening the hard mass relieving constipation; Taking Semen Persicae, Flos Carthami as nourishing blood and promoting blood circulation, helping Haematitum, Radix Angelicae Sinensis passage, nourishing blood is adjuvant drug; Rhizoma Cimicifugae is that yang invigorating helps spleen, draws all medicines and enters spleen, lung two warps, is just accord with the opinion of " The spleen has the function to transport and transform nutrients " and " clear respectful gas can not be descending in large intestine for the dryness of the lung "; Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription.All medicines share, play altogether nourish blood, effect of blood stasis expelling, relaxing the bowels with lubricant drugs.Modern medicine shows, the main component in Radix Et Rhizoma Rhei with catharsis effect is anthraquinone glycoside and dianthracene ketoside, its discharge function act as by force compared with its relevant aglycone, and anthraquinone glycoside has: chrysophanol-1-glucoside or chrysophan, emodin-6-glucoside, aloe-emodin-8-glucoside, Physcione-monoglucoside, chrysophanic acid-8-glucoside.The diarrhea inducing effect of Radix Et Rhizoma Rhei is directly proportional to associativity Rhein Content wherein, and free anthraquinone component is without catharsis effect.The compound Chinese medicinal preparation technology controlling and process for the treatment of constipation prepared by the present invention is simple, and bioavailability is high, good drug efficacy, and curative effect is fast, and cost is low.
Below data declaration beneficial effect of the present invention by experiment.In experiment, Chinese medicine preparation used is the preparation obtaining prepared by embodiment 2.
effect experiment:
1, the impact on test-type mice with constipation intestinal propulsion function:
1.1 experimentations:
group one:
Get 50 of mices, fasting be can't help after water 12h, and random packet is as table 1.Except normal group, the equal lumbar injection adrenalin hydrochloride of each treated animal 0.01mL.g
-1after, gastric infusion immediately.After 10min, cervical vertebra dislocation is put to death, and measures small intestinal total length and pylorus to prepared Chinese ink Front distance, calculates intestinal propulsion percentage rate.Adopt
tcheck analysis, result is as shown in table 1 is the impact of Chinese traditional compound medicine on epinephrine load mouse small intestine ahead running.
Table 1 result shows, epinephrine group and normal group comparison, its intestinal propulsion rate obviously reduce (
p< 0.01).Administration group and the comparison of epinephrine group, intestinal propulsion percentage rate compared with epinephrine group obviously accelerate (
p< 0.01), point out this medicine to cause that to epinephrine intestinal propulsion suppresses to have antagonism, and there is obvious dose-effect relationship.
group two:
Get 50 of mices, water 12h is can't help in fasting, is divided at random 5 groups as table 2.Administration group is the 1d each dosage Chinese traditional compound medicine of gavage aqueous solution in advance before experiment, and Normal group and morphine group are to equal-volume distilled water.15min before experiment administration on the same day, morphine group and administration group lumbar injection morphine hydrochloride (5mg.kg
-1), normal group injecting normal saline, 10 min after administration, put to death animal, measure and calculate intestinal propulsion rate.Adopt
tcheck analysis.Result is as shown in table 2 is the impact that Chinese traditional compound medicine advances morphine hydrochloride load mouse small intestine.
The data of table 2 show, morphine group intestinal propulsion percentage rate compared with normal matched group obviously reduce (
p< 0.01), 3 dosage group intestinal propulsion percentage rate of administration compared with morphine group obviously accelerate (
p< 0.01), point out the intestinal propulsion that this medicine causes morphine to suppress there is obvious antagonism, and there is certain dose-effect relationship.
group three:
Get 50 of mices, water 12h is can't help in fasting, is divided at random 5 groups as table 3.Administration group is the each dosage Chinese traditional compound medicine of gavage 1d in advance, and Normal group and atropine group are to equal-volume distilled water.15min before experiment administration on the same day, blank group and administration group lumbar injection atropine (0.02mg.kg
-1), normal group injecting normal saline.10min after administration, puts to death animal, measures and calculate intestinal propulsion rate.Adopt
tcheck analysis.Result is as shown in table 3 is the impact that Chinese traditional compound medicine advances atropine load mouse small intestine.
Wherein, △ △ and Normal group comparison
p< 0.01; * and the comparison of atropine group
p< 0.01.
The data of table 3 show, atropine group intestinal propulsion percentage rate compared with normal matched group obviously reduce (
p< 0.01), 3 dosage group intestinal propulsion percentage rate of administration compared with atropine group obviously accelerate (
p< 0.01), point out the intestinal propulsion that this medicine causes atropine to suppress there is remarkable antagonism, and there is obvious dose-effect relationship.
group four:
Get 50 of mices, be divided at random 5 groups, 10 every group, wherein press literature method modeling for 4 groups, mice gavages 10% suspension 1mL/ that makes with self feces, 4d continuously every day.1h after last administration, gavages respectively 3 dosage (26.52g.kg of Chinese traditional compound medicine aqueous solution using 3 groups as administration treated animal
-1, 13.26g.kg
-1and 6.63g.kg
-1, in crude drug, lower same).The 4th group is model group, and model group and normal group are to equal-volume normal saline.All contain 2% prepared Chinese ink in the medicinal liquid of giving and normal saline.After administration, after 10min, cervical vertebra dislocation is put to death, and measures small intestinal total length and pylorus to prepared Chinese ink Front distance, calculates intestinal propulsion percentage rate.Adopt
tcheck analysis.Result is as shown in table 4 is the impact of Chinese traditional compound medicine on excess-heat type mice with constipation intestinal propulsion.
Wherein, △ △ and Normal group comparison
p< 0.01; * and model group comparison
p< 0.01.
The data of table 4 show, model group intestinal propulsion percentage rate compared with normal matched group obviously reduce (
p< 0.01), and the hardening of model group feces quality, outward appearance is garden pearl, and hints model is reliable, and modeling success is described.3 dosage group intestinal propulsion percentage rate of administration compared with model group obviously accelerate (
p< 0.01), the obvious deliquescing of feces quality, points out this medicine to have and significantly add fastoperation excess-heat type mice with constipation intestinal propulsion function, and has certain dose-effect relationship.
group five:
Get 50 of mices, be divided at random 5 groups, grouping is in table 5, and wherein 1 group of mice is as normal group, and routine is raised.All the other 4 groups of modelings, prohibit water and only feed 3d with rice.Before experiment, water 12h is prohibited in the equal fasting of each group mice.Administration treated animal gavages respectively 3 dosage of Chinese traditional compound medicine aqueous solution, and model group and normal group are to equal-volume distilled water.All contain 2% prepared Chinese ink in the medicinal liquid of giving and water.After administration, after 10min, cervical vertebra dislocation is put to death, and measures intestinal propulsion percentage rate.After mice administration, mice is put in Mus basin, the lower berth absorbent paper of Mus basin, observes and records mice and occur first incubation period and the interior total number of particles occurring containing powdered carbon feces of 6h containing powdered carbon feces.Result is as shown in table 5 is the impact of Chinese traditional compound medicine on intestinal movement function dryness accumulated in the stomach and intestine model.
As can be seen from Table 5, the large and small enterokinesia of model group mice, make the obvious compared with normal group of powdered carbon ink propulsive rate reduce (
p< 0.01), modeling success is described.High, medium and low 3 dosage compound Chinese medicinal preparation groups all can obviously promote the large and small enterokinesia of dryness accumulated in the stomach and intestine type Constipation Model mice, make powdered carbon ink propulsive rate obviously improve (
p<0.01), occur first obviously shortening the incubation period that contains powdered carbon feces (
pthe total number of particles showed increased containing powdered carbon feces that <0.01), makes to discharge in 6h (
p<0.01).
group six:
Get 50 of mices, be divided at random 5 groups: be respectively Chinese traditional compound medicine high, medium and low 3 dosage groups, model group and Normal groups.Water 12h is can't help in animal fasting, and administration group and model group gavage give compound diphenoxylate (DC) suspension 50mg.kg
-1, normal group is given equal-volume distilled water.After 30 min, administration treated animal gavage gives tested medicine, and model group and normal group are to equal-volume distilled water.All contain 2% prepared Chinese ink in the medicinal liquid of giving and water.After administration, after 15min, cervical vertebra dislocation is put to death, and measures intestinal propulsion percentage rate.Adopt
tcheck analysis.Result is as shown in table 6 is the impact of Chinese traditional compound medicine on DC model mice defecation.
As can be seen from Table 6, the intestinal propulsion percentage rate compared with normal group of DC model mice obviously reduces, and modeling success is described.High, medium and low 3 the dosage groups of compound Chinese medicinal preparation and model group comparison small intestinal ink propulsive rate significantly increase, and illustrate that this liquid medicine solution has obvious facilitation to DC model mice intestinal propulsion function.
, impact on test-type mice with constipation defecation:
2.1 experimentations:
group seven:
Get 60 of mices, be divided at random 6 groups, grouping is in table 7, and wherein 1 group of mice is as normal group, and routine is raised.All the other 5 groups of modelings, prohibit water and only feed 3d with rice.Before experiment, water 12h is prohibited in the equal fasting of each group mice.Administration treated animal gavages respectively 3 dosage of Chinese traditional compound medicine aqueous solution.(26.52g.kg
-1, 13.26g.kg
-1and 6.63g.kg
-1, in crude drug, lower with), constipation relieving preparation (14.7g.kg
-1), model group and normal group are to equal-volume distilled water.All contain 2% prepared Chinese ink in the medicinal liquid of giving and water.After administration, mice is placed in Continuous Observation 4h in the bell jar that is covered with filter paper, and record mice the 1st row's melena time, fecal grains and weigh argol weight, the difference of more each treated animal These parameters, and use
tcheck analysis.Result is as shown in table 7 is the impact of compound Chinese medicinal preparation on dryness accumulated in the stomach and intestine model mice defecation.
As can be seen from Table 7, model group mice, discharge melena time compared with normal treated animal its 1st time and obviously increase (P < 0.01), defecation grain number, the equal compared with normal treated animal of defecation weight obviously reduce (P < 0.01), and modeling success is described.The aqueous solution of Chinese traditional compound medicine makes the 1st row's melena time of dryness accumulated in the stomach and intestine model mice obviously shorten (P < 0.01), fecal grains and stool weight obviously increase (P < 0.01), illustrate that this drug solns has good promotion defecation to dryness accumulated in the stomach and intestine type mice with constipation, and there is certain dose-effect relationship.
group eight:
50 mices are divided into 5 groups at random, 10 every group, wherein press literature method modeling for 4 groups, mice gavages 10% suspension 1mL/ that makes with self feces, 4d continuously every day.1h after last administration, gavages respectively 3 dosage (26.52g.kg of compound Chinese medicinal preparation aqueous solution using 3 groups as administration treated animal
-1, 13.26g.kg
-1and 6.63g.kg
-1, in crude drug, lower same).The 4th group is model group, and model group and normal group are to equal-volume normal saline.All contain 2% prepared Chinese ink in the medicinal liquid of giving and normal saline.After administration, mice is placed in Continuous Observation 4h in the bell jar that is covered with filter paper, and record mice the 1st row's melena time, fecal grains and weigh argol weight, the difference of more each treated animal These parameters, and use
tcheck analysis.Result is as shown in table 8 is the impact of compound Chinese medicinal preparation on excess-heat type mice with constipation bowel movement function.
As can be seen from Table 8, model group mice, its 1st time discharge melena time compared with normal normal saline treated animal obviously increase (
p< 0.01), defecation grain number, the equal compared with normal treated animal of defecation weight obviously reduce (
p< 0.01), and the hardening of model group feces quality, outward appearance is garden pearl, and hints model is reliable, and modeling success is described.Compound Chinese medicinal preparation aqueous solution make the 1st time of excess-heat model mice row's melena time obviously shorten (
p< 0.01), fecal grains and stool weight obviously increase (
p< 0.01); Said preparation aqueous solution high dose group (26.52g.kg
-1) can obviously increase defecation number and the defecation Mus number of excess-heat Constipation Model mice, and can increase stool weight, make the deliquescing of feces quality; Illustrate that this drug solns has good promotion defecation to dryness accumulated in the stomach and intestine type mice with constipation.And the 1st discharge melena time, stool weight impact are had to certain dose-effect relationship, on significantly dose-effect relationship of fecal grains impact nothing.
group nine:
Get 60 of mices, be divided at random 6 groups: be respectively the high, medium and low dosage of compound Chinese medicinal preparation (26.52 g.kg
-1, 13.26g.kg
-1, 6.63g.kg
-1) group; Positive drug constipation relieving preparation group; Model group and Normal group.Water 12h is can't help in animal fasting, and gavage gives DC suspension 50mg.kg
-1, normal group is given equal-volume distilled water.After 30 min, each treated animal gavage gives tested medicine, and administration volume is 25mL.kg
-1body weight, model group and normal group are to equal-volume distilled water.The argol that every group of mice of observed and recorded discharged is counted, loose stool is counted and the number of animals of defecation not, Continuous Observation 12h, and the difference of the These parameters of more each treated animal, and use
tcheck analysis.Result is as shown in table 9 is the impact of compound Chinese medicinal preparation on DC model mice defecation.
As can be seen from Table 9, the defecation of DC model mice count and not the number of animals compared with normal treated animal of defecation obviously reduce, modeling success is described.Effect and the model group comparison of compound Chinese medicinal preparation aqueous solution to DC model mice, the argol of its discharge count showed increased (
p< 0.05), loose stool count increase (
p< 0.01), defecation number of animals does not obviously reduce.Illustrate that this liquid medicine solution has obvious promotion defecation to DC model mice.
conclusion:
In the U.S., just exceed 2,500,000, the patient who more takes aperient in addition with the constipation medical patient that is chief complaint.The use self-appraisal way of questionnaires such as domestic Dai Fei are investigated 1535 healthy populations, and chronic constipation crowd's total prevalence rate is 9.18%, and male is 7.28%, and women is that more than 11.04%, 60 years old person is 14.84%.Prolonged constipation is very harmful, especially for having a heart disease, the gerontal patient of cerebrovascular, can bring out the diseases such as heart failure or rupture of blood vessel in brain are hemorrhage, often can threat to life.And compound Chinese medicinal preparation has effect of the blood stasis expelling of nourishing blood, relaxing the bowels with lubricant drugs relieving constipation, treatment chronic senile constipation determined curative effect.Above-mentioned mouse experiment is to cure mainly according to the function of this Chinese traditional compound medicine, observe the impact of this medicine on the dehydration dryness accumulated in the stomach and intestine type similar to clinical symptoms and excess-heat type Constipation Model and non-specific constipation (DC) model mice defecation, result shows that this medicine all has significant promotion defecation to above-mentioned 3 kinds of Constipation Model mices, illustrates that this medicine has obvious promotion defecation effect.
Detailed description of the invention
embodiment 1:
Treat a compound Chinese medicinal preparation for constipation, described preparation is made up of the raw material of following weight proportion: Haematitum 5g, Radix Angelicae Sinensis 10g, Matrii Sulfas Exsiccatus 3g, Radix Rehmanniae Preparata 15g, Radix Rehmanniae 15g, Radix Et Rhizoma Rhei 3g, Fructus Cannabis 15g, Flos Carthami 5g, Semen Persicae 5g, Rhizoma Cimicifugae 1g, Radix Glycyrrhizae 1g.
Said preparation is granule.
The preparation technology of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, step is as follows:
(1) take raw material Radix Angelicae Sinensis, Flos Carthami according to proportional quantity, after pulverizing, crossing aperture is 20 objects sieves, and adding quality is the water soaking 0.5 hour of 4 times, powder after sieving, and adopts vapor distillation 5 hours, collect volatile oil by volatile oil extractor, isolated by filtration residue medicinal liquid and medicinal residues;
(2) take raw material Haematitum, Radix Rehmanniae Preparata, Radix Rehmanniae, Rhizoma Cimicifugae and Radix Glycyrrhizae according to proportional quantity, after pulverizing, crossing aperture is 20 object sieves, add the medicinal residues that obtain in step (1), add water and reflux 3 times, the water adding for 3 times is respectively 5 times, 3 times and 3 times of medicinal residues quality, refluxes 0.5 hour at every turn, and reflux temperature is 100oC, after extraction finishes, isolated by filtration medicinal liquid separates with medicinal residues;
(3) medicinal liquid of step (1) and (2) gained is merged, precipitate 8 hours with 50% ethanol, get clear liquid medicine;
(4) take raw material Radix Et Rhizoma Rhei, Semen Persicae and Fructus Cannabis according to proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adds 50% alcohol reflux 3 times, add ethanol quality is 6 times, 4 times and 4 times of medicinal powder quality at every turn, each backflow 20 minutes, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues;
(5) medicinal liquid of step (3) and (4) gained is merged, be condensed into relative density and be 1.14 extractum, then, 40 DEG C of vacuum dryings 10 hours, pulverize and obtain extract powder;
(6) in volatile oil step (1) being obtained, adding quality is the cyclodextrin of 6 times of volatile oil, and enclose temperature 50 C, makes volatile oil clathrate compound for 1 hour;
(7) volatile oil clathrate compound step (6) being obtained mixes with the extract powder that step (5) obtains, then adds soluble starch, is 0.5 times of mixed-powder quality, then makes soft material after adding adhesive;
(8) it is 14 objects sieves granulations that soft material step (7) being obtained is crossed aperture, by wet granular 40-45 DEG C dry 1 hour after with 20 mesh sieve granulate, subpackage, obtain granule.
embodiment 2
Treat a compound Chinese medicinal preparation for constipation, described preparation is made up of the raw material of following weight proportion: Haematitum 9g, Radix Angelicae Sinensis 15g, Matrii Sulfas Exsiccatus 4.5g, Radix Rehmanniae Preparata 15g, Radix Rehmanniae 20g, Radix Et Rhizoma Rhei 4.5g, Fructus Cannabis 20g, Flos Carthami 6g, Semen Persicae 9g, Rhizoma Cimicifugae 3g, Radix Glycyrrhizae 3g.
Said preparation is granule.
The preparation technology of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, step is as follows:
(1) take raw material Radix Angelicae Sinensis, Flos Carthami according to proportional quantity, after pulverizing, crossing aperture is 20 objects sieves, and adding quality is the water soaking 1 hour of 8 times, powder after sieving, and adopts vapor distillation 5 hours, collect volatile oil by volatile oil extractor, isolated by filtration residue medicinal liquid and medicinal residues;
(2) take raw material Haematitum, Radix Rehmanniae Preparata, Radix Rehmanniae, Rhizoma Cimicifugae and Radix Glycyrrhizae according to proportional quantity, after pulverizing, crossing aperture is 20 object sieves, add the medicinal residues that obtain in step (1), add water and reflux 3 times, the water adding for 3 times is respectively 8 times, 6 times and 6 times of medicinal residues quality, refluxes 0.5 hour at every turn, and reflux temperature is 100oC, after extraction finishes, isolated by filtration medicinal liquid separates with medicinal residues;
(3) medicinal liquid of step (1) and (2) gained is merged, precipitate 10 hours with 50% ethanol, get clear liquid medicine;
(4) take raw material Radix Et Rhizoma Rhei, Semen Persicae and Fructus Cannabis according to proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adds 50% alcohol reflux 3 times, add ethanol quality is 7 times, 5 times and 5 times of medicinal powder quality at every turn, each backflow 20 minutes, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues;
(5) medicinal liquid of step (3) and (4) gained is merged, be condensed into relative density and be 1.14 extractum, then, 50 DEG C of vacuum dryings 10 hours, pulverize and obtain extract powder;
(6) in volatile oil step (1) being obtained, adding quality is the cyclodextrin of 6 times of volatile oil, and enclose temperature 50 C, makes volatile oil clathrate compound for 1 hour;
(7) volatile oil clathrate compound step (6) being obtained mixes with the extract powder that step (5) obtains, then adds soluble starch, is 0.5 times of mixed-powder quality, then makes soft material after adding adhesive;
(8) it is 14 objects sieves granulations that soft material step (7) being obtained is crossed aperture, by wet granular 40-45 DEG C dry 1 hour after with 20 mesh sieve granulate, subpackage, obtain granule.
embodiment 3
Treat a compound Chinese medicinal preparation for constipation, described preparation is made up of the raw material of following weight proportion: Haematitum 10g, Radix Angelicae Sinensis 15g, Matrii Sulfas Exsiccatus 10g, Radix Rehmanniae Preparata 20g, Radix Rehmanniae 25g, Radix Et Rhizoma Rhei 10g, Fructus Cannabis 25g, Flos Carthami 10g, Semen Persicae 10g, Rhizoma Cimicifugae 10g, Radix Glycyrrhizae 10g.
Said preparation is granule.
The preparation technology of above-mentioned a kind of compound Chinese medicinal preparation for the treatment of constipation, step is as follows:
(1) take raw material Radix Angelicae Sinensis, Flos Carthami according to proportional quantity, after pulverizing, crossing aperture is 20 objects sieves, and adding quality is the water soaking 1 hour of 10 times, powder after sieving, and adopts vapor distillation 12 hours, collect volatile oil by volatile oil extractor, isolated by filtration residue medicinal liquid and medicinal residues;
(2) take raw material Haematitum, Radix Rehmanniae Preparata, Radix Rehmanniae, Rhizoma Cimicifugae and Radix Glycyrrhizae according to proportional quantity, after pulverizing, crossing aperture is 20 object sieves, add the medicinal residues that obtain in step (1), add water and reflux 3 times, the water adding for 3 times is respectively 10 times, 8 times and 8 times of medicinal residues quality, refluxes 0.5 hour at every turn, and reflux temperature is 100oC, after extraction finishes, isolated by filtration medicinal liquid separates with medicinal residues;
(3) medicinal liquid of step (1) and (2) gained is merged, precipitate 10 hours with 50% ethanol, get clear liquid medicine;
(4) take raw material Radix Et Rhizoma Rhei, Semen Persicae and Fructus Cannabis according to proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adds 50% alcohol reflux 3 times, add ethanol quality is 8 times, 6 times and 6 times of medicinal powder quality at every turn, each backflow 20 minutes, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues;
(5) medicinal liquid of step (3) and (4) gained is merged, be condensed into relative density and be 1.14 extractum, then, 60 DEG C of vacuum dryings 10 hours, pulverize and obtain extract powder;
(6) in volatile oil step (1) being obtained, adding quality is the cyclodextrin of 6 times of volatile oil, and enclose temperature 50 C, makes volatile oil clathrate compound for 1 hour;
(7) volatile oil clathrate compound step (6) being obtained mixes with the extract powder that step (5) obtains, then adds soluble starch, is 0.5 times of mixed-powder quality, then makes soft material after adding adhesive;
(8) it is 14 objects sieves granulations that soft material step (7) being obtained is crossed aperture, by wet granular 40-45 DEG C dry 1 hour after with 20 mesh sieve granulate, subpackage, obtain granule.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (6)
1. a preparation method for the treatment of the compound Chinese medicinal preparation of constipation, is characterized in that, comprises the following steps:
1) preparation is made up of the raw material of following weight proportion: 9 parts of Haematitums, 15 parts of Radix Angelicae Sinensis, 4.5 parts of Matrii Sulfas Exsiccatus, 15 parts of Radix Rehmanniae Preparata, 20 parts of Radix Rehmanniae, 4.5 parts of Radix Et Rhizoma Rhei, 20 parts of Fructus Cannabiss, 6 parts, Flos Carthami, 9 parts, Semen Persicae, 3 parts of Rhizoma Cimicifugaes, 3 parts, Radix Glycyrrhizae;
Take Radix Angelicae Sinensis, the Flos Carthami in raw material according to above-mentioned proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adding quality is powder 2-10 water soaking 0.5-2 hour doubly after sieving, adopt vapor distillation 5-15 hour, collect volatile oil by volatile oil extractor, the remaining medicinal liquid of isolated by filtration and medicinal residues, obtain medicinal liquid one;
2) take Haematitum, Radix Rehmanniae Preparata, Radix Rehmanniae, Rhizoma Cimicifugae and the Radix Glycyrrhizae in raw material according to above-mentioned proportional quantity, after pulverizing, crossing aperture is 20 object sieves, add the medicinal residues that obtain in step 1), add water and reflux 3 times, the water at every turn adding is 3-10 times of medicinal residues quality, each backflow 0.5-2 hour, reflux temperature is 100
oc, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues, obtain medicinal liquid two;
3) medicinal liquid one and the step 2 that step 1) are obtained) medicinal liquid two merging that obtain, with quality percentage composition be 60-80% ethanol precipitation 8-10 hour, get clear liquid medicine, obtain medicinal liquid three;
4) take raw material Radix Et Rhizoma Rhei, Semen Persicae and Fructus Cannabis according to above-mentioned proportional quantity, after pulverizing, crossing aperture is 20 object sieves, adding quality percentage composition is 50-80% alcohol reflux 3 times, add at every turn ethanol quality be medicinal powder 2-10 doubly, each backflow 0.3-2 hour, after extraction finishes, isolated by filtration medicinal liquid and medicinal residues, obtain medicinal liquid four;
5) medicinal liquid four merging that the medicinal liquid three step 3) being obtained and step 4) obtain, are condensed into the extractum that relative density is 1.28-1.36, then 50 DEG C of vacuum dryings 10 hours, pulverize and obtain extract powder;
6) in volatile oil step 1) being obtained, adding quality is that the cyclodextrin of 6 times of volatile oil carries out clathration, and enclose temperature is 60 DEG C, and enclose makes volatile oil clathrate compound for 1 hour;
7) take raw material Matrii Sulfas Exsiccatus according to above-mentioned proportional quantity, after pulverizing, crossing aperture is 20 object sieves, after the extract powder obtaining with step (5), the volatile oil clathrate compound that step 6) obtains mix, add the soluble starch of 0.5 times of mixed-powder quality, then make soft material after adding adhesive;
8) it is that 14 object sieve series become wet granular that soft material step 7) being obtained is crossed aperture, and wet granular is dried after 1 hour with 20 mesh sieve granulate, subpackage at 40-45 DEG C, obtains granule.
2. the preparation method of a kind of compound Chinese medicinal preparation for the treatment of constipation according to claim 1, is characterized in that, in described step 1), is that to add quality be the water soaking 1 hour of 8 times, powder after sieving, and adopts vapor distillation 10 hours.
3. the preparation method of a kind of compound Chinese medicinal preparation for the treatment of constipation according to claim 2, is characterized in that, described step 2) add water and reflux in 3 processes, the water adding for 3 times is respectively 8 times, 6 times and 6 times of medicinal residues quality, refluxes 1 hour at every turn.
4. the preparation method of a kind of compound Chinese medicinal preparation for the treatment of constipation according to claim 3, is characterized in that,
In described step 3), be the ethanol precipitation that is 70% with quality percentage composition 9 hours;
In described step 4), be that to add quality percentage composition be 60% alcohol reflux 3 times, add for 3 times the quality of 60% ethanol to be respectively 7 times, 6 times and 6 times of medicinal residues quality, 20min refluxes at every turn.
5. the preparation method of a kind of compound Chinese medicinal preparation for the treatment of constipation according to claim 4, is characterized in that, the cyclodextrin in described step 6) is beta-schardinger dextrin-.
6. the preparation method of a kind of compound Chinese medicinal preparation for the treatment of constipation according to claim 5, is characterized in that, the adhesive in described step 7) is 80% ethanol.
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| JPH09121810A (en) * | 1995-08-03 | 1997-05-13 | Hida Aroe:Kk | Granular health food produced by compounding powder of aloe arborescens and powder of angelica utilis |
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| KR100720695B1 (en) * | 2004-06-02 | 2007-05-29 | 주식회사 브레인가드 | Composition for treating and preventing constipation |
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