CN103083206A - Sweet wormwood herb extract, preparation method and uses thereof - Google Patents
Sweet wormwood herb extract, preparation method and uses thereof Download PDFInfo
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- CN103083206A CN103083206A CN2011103336576A CN201110333657A CN103083206A CN 103083206 A CN103083206 A CN 103083206A CN 2011103336576 A CN2011103336576 A CN 2011103336576A CN 201110333657 A CN201110333657 A CN 201110333657A CN 103083206 A CN103083206 A CN 103083206A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention belongs to the field of chemical and daily chemical products, and relates to a sweet wormwood herb extract, a preparation method and uses thereof. Specifically the sweet wormwood herb extract preparation method comprises adopting supercritical CO2 extraction, wherein a used entrainer is peppermint oil. The present invention further relates to a composition containing the sweet wormwood herb extract, mosquito repelling daily chemical products and mosquito repelling agents, a use of the peppermint oil as the entrainer for preparing the sweet wormwood herb extract through supercritical CO2 extraction, and a mosquito repelling method. The sweet wormwood herb extract can be used for preparing mosquito repelling daily chemical products or mosquito repelling agents, and has a good mosquito repelling effect. In addition, the sweet wormwood herb extract has low viscosity, and is not easily adhered to equipment pipelines, wherein extraction efficiency is high. The mosquito repelling daily chemical products or the mosquito repelling agents have characteristics of good mosquito repelling effect, long mosquito repelling time, safety and no toxicity.
Description
Technical field
The invention belongs to chemistry and daily chemical products field, relate to a kind of Herba Artemisiae Annuae extract, Preparation Method And The Use.The invention still further relates to the compositions, anophelifuge daily chemical products and the anophelifuge that contain this Herba Artemisiae Annuae extract, Oleum menthae is as supercritical CO
2The purposes of the entrainer in extraction preparation Herba Artemisiae Annuae extract, and a kind of anophelifuge method.
Background technology
Domestic and international widely used anophelifuge has floral water, essential balm, mosquito incense etc. at present, although they have good repelling effect, but it is synthetic that the effective ingredient of most of product wherein is artificial chemistry, often contains the harmful substances such as benzene, if long-term suction meeting damages human body.
The effective ingredient of mosquito repellent product belongs to pesticide mostly at present, the part mosquito repellent product all carries out clear and definite mark to its contained effective ingredient and content at present, as meeting mark printed words such as " dimefluthrin content: 0.62% " in some mosquito incense liquid, and most mosquito incense has also identified " little poison " printed words.
The effective ingredient of most of mosquito incenses (0.2%-0.4%) is the pyrethrin insecticide.Containing the ultrafine dust particulate matter of 2.5 microns (diameter less than) in the cigarette of mosquito incense burning can enter and stay in lung, therefore, may cause asthma in a short time, for a long time may cause cancer; Polycyclic aromatic hydrocarbon (PAHs), they are mosquito incense base material products of incomplete combustion, belonging to may carcinogen.
Though the tradition of China's always useful plant among the people anophelifuge, most methods that adopt the plant sootiness, the method are limited by the raw material source place, and in the family of easily igniting, thing is assorted, has hidden danger, and long one offending smoke that leaves also between the sootiness back room.
Therefore, need a kind of mosquito repellent product of safety non-toxic badly.
Supercritical fluid extraction is the new technique that occurs in the chemical separating in modern age, is called for short SFE.At a lower temperature, when constantly increasing the pressure of gas, gas can change into liquid, when increase in temperature, the volume of liquid increases, for a certain specific material, a total critical temperature (Tc) and critical pressure (Pc) of existing, after critical temperature and critical pressure, material can not become liquid or gas, and this point is exactly critical point.In scope more than critical point, state of matter is between gas and liquid, and the fluid within this scope becomes supercritical fluid (SF).Supercritical fluid have similar gas than strong penetrating power and be similar to greater density and the dissolubility of liquid, have good solvent properties, can be used as that solvent extracts, separating monomer.
SFE integrally combines traditional distillation and organic solvent extraction, utilizes supercritical CO
2Good solvent power, with substrate effectively separate with extract, extraction and purification.SFE uses supercritical CO
2Material is extracted.CO
2Safe, nontoxic, cheap liquid, supercritical CO
2Have the diffusion coefficient of similar gas, the dissolving power of liquid, surface tension is zero, can advance among solid matter by rapid permeability, extracts its elite, have efficient, be difficult for oxidation, pure natural, without characteristics such as chemical contaminations.
When using pure gas, dissolubility or selectivity often are subject to certain restrictions, and at this moment can select with the affine strong component of extract to add supercritical fluid, and to improve its selectivity and dissolubility to being extracted component, this class material is called entrainer.The principle of entrainer effect is that entrainer can affect from two aspects dissolubility and the selectivity of solute supercritical fluid, the i.e. density of solvent fluid and solute and entrainer Molecular interaction.
Usually in use consumption is less for entrainer, Effects of Density to solvent fluid is little, even also can reduce the density of supercritical fluid, and affect dissolubility and determiner be exactly that Van der Waals force between entrainer and solute molecule or entrainer and solute have specific intermolecular electing property use, as hydrogen bond, weak complexation and other motive powers.At the Near The Critical Point of solvent, solute solubility is the most responsive to the variation of temperature, pressure, after adding entrainer, can make the corresponding change of critical point of mixed solvent, more near extraction temperature in addition.Strengthen solute solubility to the sensitivity of temperature, pressure, separated component is separated from recyclegas by temperature, pressure, to avoid the high energy consumption of gas recompression, entrainer not only can increase dissolubility and the selectivity of solute in supercritical fluid, can also be conducive to as cosurfactant the formation of supercritical fluid microemulsion simultaneously.
Relevant for utilizing SFE to extract the report of arteannuin, it has mainly inquired into pressure, time, CO at present
2Impact (Ge Fahuan, Zhang Jingcheng, display, the supercritical CO of Artemisinin from Artemisia Annua L of the factors such as flow on product yield
2The fluid extraction technical study. Chinese Journal of Pharmaceuticals, 2000,31 (6): 250-253; Li Shu supports, Wu Zuxiang, Huang Jian etc., the research of arteannuin production technology [J]. Chinese herbal medicine communication, 1979, (12): 11-15.)。
However, still need and want new Herba Artemisiae Annuae extract, its when itself is nontoxic, effective anophelifuge, and extraction efficiency is higher.
Summary of the invention
The inventor has obtained a kind of preparation method of Herba Artemisiae Annuae extract through deep research and performing creative labour.And the inventor is surprised to find, and the Herba Artemisiae Annuae extract that the method makes can be used in preparation anophelifuge daily chemical products or anophelifuge and has good repelling effect; In addition, the inventor also finds, Herba Artemisiae Annuae extract viscosity of the present invention is low, is not easy to be bonded in equipment pipe, and extraction efficiency is high.Following invention is provided thus:
One aspect of the present invention relates to a kind of preparation method of Herba Artemisiae Annuae extract, comprises the use supercritical CO
2The step of extraction, wherein, entrainer used is Oleum menthae.Particularly, described preparation method is supercritical CO
2Extraction.
The described preparation method of any one according to the present invention, wherein, the consumption of described Oleum menthae is the 0.1%-30% of Herba Artemisiae Annuae weight; Preferably, be 0.2%-20%; More preferably, be 0.5%-10%; Further preferably, be 1%-5%, for example 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5%.
The described preparation method of any one according to the present invention is characterized in that any one in following (1)-(6) or multinomial:
(1) extracting pressure is 15-35MPa; Preferably, be 18-35MPa; More preferably, be 22-35MPa; Further preferably, be 28-32MPa;
(2) extraction temperature is 40 ℃-70 ℃; Preferably, be 45-69 ℃; More preferably, be 50-68 ℃; Further preferably, be 55-65 ℃;
(3) extraction time is 1-5 hour; Preferably, be 1-4 hour; More preferably, be 1.5-3.5 hour; Further preferably, be 2-3 hour;
(4) resolving pressure is 5-10MPa; Preferably, be 6-10MPa; More preferably, be 6.5-9.5MPa; Further preferably, be 7-9MPa;
(5) resolution temperature is 40 ℃-70 ℃; Preferably, be 50-70 ℃; More preferably, be 60-70 ℃; Further preferably, be 60-68 ℃.
The described preparation method of any one according to the present invention, wherein, Herba Artemisiae Annuae used is through pulverizing.
The described preparation method of any one according to the present invention, wherein, the mean diameter after Herba Artemisiae Annuae is pulverized is the 10-80 order, is preferably the 24-65 order; 24-50 order more preferably.
The described preparation method of any one according to the present invention, wherein, Herba Artemisiae Annuae used meets the requirement of Chinese Pharmacopoeia (for example Chinese Pharmacopoeia of version in 2010).
Another aspect of the present invention relates to the Herba Artemisiae Annuae extract that the described preparation method of any one makes according to the present invention.
Of the present inventionly relate in one aspect to a kind of compositions, it comprises Herba Artemisiae Annuae extract of the present invention again; Alternatively, described compositions also comprises pharmaceutically or the upper acceptable carrier of physiology or adjuvant.Particularly, described compositions is for being used for the compositions of anophelifuge.
Anophelifuge daily chemical products or the anophelifuge that comprises Herba Artemisiae Annuae extract of the present invention, for example bath foam, cosmetic water, handwashing liquid, fancy soap, perfume, the mosquito-repellent water of relating in one aspect to again of the present invention.
The described anophelifuge daily chemical products of any one or anophelifuge according to the present invention, wherein, the content of described Herba Artemisiae Annuae extract is 0.1%-10% (w/w).
The described anophelifuge daily chemical products of any one or anophelifuge according to the present invention, its component and as follows according to the content of weight percent meter:
First type surface activating agent 10%-20%, auxiliary surfactant 0%-8%, suds booster 2%-5%, pH adjusting agent is considered, viscosity modifier 0%-0.5%, pearling agent 0%-2%, stabilizing agent 0.05%-1%, essence 0-2% adds water to 100%.
The described anophelifuge daily chemical products of any one or anophelifuge according to the present invention, wherein,
described first type surface activating agent is selected from: fatty alcohol sulfate sodium, the fatty alcohol sulfate ammonium, the fatty alcohol sulfate triethanolamine, polyoxyethylene alkyl ether sulfate sodium, polyoxyethylene alkyl ether sulfate, polyoxyethylene alkyl ether sulfate magnesium, the polyoxyethylene alkyl ether sulfate triethanolamine, sulfosuccinate sodium, the sulfosuccinate ammonium, the sulfosuccinate triethanolamine, sarcosinate sodium, the sarcosinate ammonium, the sarcosinate triethanolamine, N-acyl group taurine, sodium isethionate, the hydroxyethylsulfonic acid. ammonium, hydroxyethylsulfonic acid. triethanolamine etc.,
Described auxiliary surfactant is selected from: betanin, cocos nucifera oil both sexes sodium propionate, glucoside derivative etc.;
Described suds booster is selected from: alkylolamides;
Described pH adjusting agent is selected from: citric acid;
Described viscosity modifier is selected from: the two stearates of PEG-6000, PEG-50 polypropylene glycol oleate, polyacrylic resin, cellulose ether, alkylolamides, HANSHENGJIAO etc.;
Described pearling agent is selected from: ethylene glycol monostearate, Ethylene Glycol Distearate, T
iO
2Cover pigments, mica etc.;
Described stabilizing agent is selected from: parabens, imidazolidinyl urea, dehydroacetic acid, sorbic acid etc.;
Described essence is selected from: Fructus Citri Limoniae essence, Mint Essence, Fructus Citri tangerinae essence, strawberry essence etc.
The described anophelifuge daily chemical products of any one or anophelifuge according to the present invention is characterized in that any one or multinomial in the item of following (1)-(4):
(1) described first type surface activating agent is Dodecyl Polyoxyethylene Ether ammonium sulfate and fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium;
When the first type surface activating agent was selected Dodecyl Polyoxyethylene Ether ammonium sulfate (AESA, 70%) and fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium (MES), the extremely low and foaminess of their composite rear zests and rinsability were outstanding.
(2) described auxiliary surfactant is cocamido propyl betaine;
Amphoteric surfactant cocamido propyl betaine (CAB, 35%) can improve foam quality, further reduces the zest of first type surface activating agent.
(3) described suds booster and viscosity modifier are the fatty acid distribution of coconut oil diglycollic amide;
Fatty acid distribution of coconut oil diglycollic amide (6501,70%) can play suds booster and viscosity modifier simultaneously.
(4) described pH adjusting agent is citric acid.
Anophelifuge daily chemical products of the present invention or anophelifuge repelling effect are good, and safety non-toxic.
The purposes of the described Herba Artemisiae Annuae extract of any one of the present invention in preparation anophelifuge daily chemical products or anophelifuge that relate in one aspect to again of the present invention.
The Oleum menthae that relates in one aspect to again of the present invention is as supercritical CO
2The purposes of the entrainer in extraction preparation Herba Artemisiae Annuae extract.
A kind of method that relates in one aspect to again anophelifuge or prevention mosquito bite of the present invention comprises the step of the described Herba Artemisiae Annuae extract of any one of the present invention that uses effective dose, the described compositions of any one of the present invention or the described anophelifuge daily chemical products of any one of the present invention or anophelifuge.
The beneficial effect of the invention
Herba Artemisiae Annuae extract of the present invention can be used in preparation anophelifuge daily chemical products or anophelifuge, has good repelling effect, and the anophelifuge time is long; In addition, Herba Artemisiae Annuae extract viscosity of the present invention is low, is not easy to be bonded in equipment pipe, and extraction efficiency is high.Anophelifuge daily chemical products of the present invention or anophelifuge repelling effect are good, effect lasting (the anophelifuge time is long), and safety non-toxic.
The specific embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only is used for explanation the present invention, and should not be considered as limiting scope of the present invention.Unreceipted actual conditions person in embodiment carries out according to the condition of normal condition or manufacturer's suggestion.The unreceipted person of production firm of agents useful for same or instrument, being can be by the conventional products of commercial acquisition.
Embodiment 1: the preparation of Herba Artemisiae Annuae extract sample 1
Artemisia annua is pulverized and to be coarse powder (24 orders-65 order), take the Oleum menthae of medical material amount 1% as entrainer, carries out supercritical CO
2Extraction, extracting pressure is 30MPa, and extraction temperature is 60 ℃, and parsing pressure is 8MPa, and resolution temperature is 65 ℃, extraction time is 2 hours.Make Herba Artemisiae Annuae extract sample 1.
Embodiment 2: the preparation of Herba Artemisiae Annuae extract sample 2
Artemisia annua is pulverized and to be coarse powder (24 orders-65 order), take the Oleum menthae of medical material amount 0.1% as entrainer, carries out supercritical CO
2Extraction, extracting pressure is 30MPa, and extraction temperature is 60 ℃, and parsing pressure is 8MPa, and resolution temperature is 65 ℃, extraction time is 2 hours.Make Herba Artemisiae Annuae extract sample 2.
Embodiment 3: the preparation of Herba Artemisiae Annuae extract sample 3
Artemisia annua is pulverized and to be coarse powder (24 orders-65 order), take the Oleum menthae of medical material amount 15% as entrainer, carries out supercritical CO
2Extraction, extracting pressure is 30MPa, and extraction temperature is 60 ℃, and parsing pressure is 8MPa, and resolution temperature is 65 ℃, extraction time is 2 hours.Make Herba Artemisiae Annuae extract sample 3.
Embodiment 4: the preparation of Herba Artemisiae Annuae extract sample 4
Artemisia annua is pulverized and to be coarse powder (24 orders-65 order), take the Oleum menthae of medical material amount 30% as entrainer, carries out supercritical CO
2Extraction, extracting pressure is 30MPa, and extraction temperature is 60 ℃, and parsing pressure is 8MPa, and resolution temperature is 65 ℃, extraction time is 2 hours.Make Herba Artemisiae Annuae extract sample 4.
Embodiment 5: the preparation of mosquito repellent product 1 (bath foam)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 0.1%, AESA (70%) 11%, MES 5%, CAB (35%) 9%, fatty acid distribution of coconut oil diglycollic amide (6501) 3%, citric acid 0.1%, glycerol 2%, pearlescent flake 3.5%, essence, antiseptic are appropriate, surplus is deionized water.
Preparation method:
Deionized water is put into agitator, heat to 70-80 ℃, add MES and AESA, be stirred to and be dissolved into clear solution fully.Cool to 60 ℃, add except 6501 and essence other composition, stirring and dissolving is even, drops to below 40 ℃ to add essence, then adds 6501 thickenings.Make the product froth breaking more than standing 24h, the canned finished product that gets.
Embodiment 6: the preparation of mosquito repellent product 2 (bath foam)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 1%, AESA (70%) 11%, MES5%, CAB (35%) 9%, fatty acid distribution of coconut oil diglycollic amide (6501) 3%, citric acid 0.1%, glycerol 2%, pearlescent flake 3.5%, essence, antiseptic are appropriate, the deionized water surplus.
Preparation method:
Deionized water is put into agitator, heat to 70-80 ℃, add MES and AESA, be stirred to and be dissolved into clear solution fully.Cool to 60 ℃, add except 6501 and essence other composition, stirring and dissolving is even, drops to below 40 ℃ to add essence, then adds 6501 thickenings.Make the product froth breaking more than standing 24h, the canned finished product that gets.
Embodiment 7: the preparation of mosquito repellent product 3 (bath foam)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 5%, AESA (70%) 11%, MES5%, CAB (35%) 9%, fatty acid distribution of coconut oil diglycollic amide (6501) 3%, citric acid 0.1%, glycerol 2%, pearlescent flake 3.5%, essence, antiseptic are appropriate, the deionized water surplus.
Preparation method:
Deionized water is put into agitator, heat to 70-80 ℃, add MES and AESA, be stirred to and be dissolved into clear solution fully.Cool to 60 ℃, add except 6501 and essence other composition, stirring and dissolving is even, drops to below 40 ℃ to add essence, then adds 6501 thickenings.Make the product froth breaking more than standing 24h, the canned finished product that gets.
Embodiment 8: the preparation of mosquito repellent product 4 (bath foam)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 10%, AESA (70%) 11%, MES 9%, CAB (35%) 9%, fatty acid distribution of coconut oil diglycollic amide (6501) 3%, citric acid 0.1%, glycerol 2%, pearlescent flake 3.5%, essence, antiseptic are appropriate, the deionized water surplus.
Preparation method:
Deionized water is put into agitator, heat to 70-80 ℃, add MES and AESA, be stirred to and be dissolved into clear solution fully.Cool to 60 ℃, add except 6501 and essence other composition, stirring and dissolving is even, drops to below 40 ℃ to add essence, then adds 6501 thickenings.Make the product froth breaking more than standing 24h, the canned finished product that gets.
Embodiment 9: the preparation of mosquito repellent product 5 (mosquito-repellent water)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 5%, Flos Chrysanthemi Indici extract 5%, tween 10%, medical alcohol 50%, citric acid 0.1%, Mentholum 2%, essence, antiseptic are appropriate, the deionized water surplus.
Preparation method:
First mix with tween, Herba Artemisiae Annuae extract, Mentholum, slowly add pure water, make water-soluble elite, add 95% medical alcohol 40%, dilution adds glycerol, essence, antiseptic, stirs, and adds remaining deionized water, in the bottle of packing into.
Embodiment 10: the preparation of mosquito repellent product 6 (mosquito-repellent water)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 10%, tween 10%, medical alcohol 50%, citric acid 0.1%, Mentholum 2%, essence, antiseptic are appropriate, the deionized water surplus.
Preparation method:
First mix with tween, Herba Artemisiae Annuae extract, Mentholum, slowly add pure water, make water-soluble elite, add 95% medical alcohol 40%, dilution adds glycerol, essence, antiseptic, stirs, and adds remaining deionized water, in the bottle of packing into.
Embodiment 11: the preparation of mosquito repellent product 7 (mosquitofugal oil)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract 10% (embodiment 1 preparation sample 1), olive oil 90%, essence are appropriate.
Preparation method:
Herba Artemisiae Annuae extract and mixed with olive oil is even, add appropriate essence, in the bottle of packing into.
Embodiment 12: the preparation of mosquito repellent product 8 (mosquitofugal oil)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract (embodiment 1 preparation sample 1) 5%, Oleum menthae 5%, olive oil 90%, essence are appropriate.
Preparation method:
Herba Artemisiae Annuae extract, Oleum menthae, mixed with olive oil is even, add appropriate essence, in the bottle of packing into.
Embodiment 13: the preparation of mosquito repellent product 9 (mosquitofugal oil)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract 5%, Oleum Rosae Rugosae 5%, olive oil 90%.
Preparation method:
Herba Artemisiae Annuae extract, Oleum Rosae Rugosae, mixed with olive oil is even, in the bottle of packing into.
Embodiment 14: the preparation of mosquito repellent product 10
According to the method preparation identical with embodiment 5, be the Herba Artemisiae Annuae extract sample 2 of embodiment 2 preparations except Herba Artemisiae Annuae extract used.Obtain mosquito repellent product 10.
Embodiment 15: the preparation of mosquito repellent product 11
According to the method preparation identical with embodiment 5, be the Herba Artemisiae Annuae extract sample 3 of embodiment 3 preparations except Herba Artemisiae Annuae extract used.Obtain mosquito repellent product 11.
Embodiment 16: the preparation of mosquito repellent product 12
According to the method preparation identical with embodiment 5, be the Herba Artemisiae Annuae extract sample 4 of embodiment 4 preparations except Herba Artemisiae Annuae extract used.Obtain mosquito repellent product 12.
Embodiment 17: the preparation of mosquito repellent product 13 (mosquito-expelling scented soap)
Composite formula (in weight portion):
Herba Artemisiae Annuae extract 0.1% (sample 1 of embodiment 1 preparation), 30% sodium hydroxide solution 20%, olive oil 18%, Adeps Bovis seu Bubali 18%, sucrose 10%, 95% ethanol 6%, water 12.4%, glycerol 3.5%, crystal sugar 2%.
Preparation method:
Take 75 ℃ of heating of fatty acid, add 30%NaOH solution, 95% ethanol, crystal sugar, at the uniform velocity stirred 2-3 hour, add glycerol, sucrose, distilled water, stir evenly, be cooled to 60 ℃, add Herba Artemisiae Annuae extract, after continuing to stir evenly, discharging, pour cold water-cooled cold mould into, solidify rapidly, obtain the Herba Artemisiae Annuae mosquito-expelling scented soap.
Embodiment 18: repelling effect evaluation experimental 1
1. for reagent
The mosquito repellent product 1-13 that embodiment 5 to 17 is prepared.
2. for examination worm kind and strain
Aedes albopictus (Aedes albopictus), the normal strain of indoor feeding, after sprouting wings, the 4-5 days female adult mosquitos of not sucking blood are for measuring.
3. assay method
1) attack experiments experiment personnel all put on the disposable glove that the back side cuts 4cm * 4cm size windows, hands is stretched into mosquito cage that 300 mosquitoes are housed (2min in 40cm * 30cm * 30cm), the mosquito that comes to stop falling is qualified for attack more than 30 persons, and this person and this cage mosquito can be used for repellency test.
2) repellent experiments experiment personnel wear the altex glove that the back of the hand exposes 4cm * 4cm, press 1.5mgcm
-2Dosage, left hand is smeared reagent, and right hand blank stretches into hands in the mosquito cage after 2 hours and stops 2min, records respectively the mosquito bite number of times, calculates Effective Vate of Protection.
4. experimental result and conclusion
The Effective Vate of Protection of the mosquito repellent product 1-13 of embodiment 5-17 preparation all reaches more than 95%.And product of the present invention does not contain diethyltoluamide, and all components is the pure natural extract, and is safe and efficient.
Embodiment 19: the repelling effect control experiment
The experiment mosquito repellent product: Herba Artemisiae Annuae extract used is the prepared sample of embodiment 22; Method preparation according to embodiment 5.
Contrast mosquito repellent product 1: Herba Artemisiae Annuae extract used is commonsense method preparation (with reference to embodiment 2 methods, except without Oleum menthae); Method preparation according to embodiment 5.
Contrast mosquito repellent product 2: Herba Artemisiae Annuae extract used is commonsense method preparation (with reference to embodiment 2 methods, except without Oleum menthae); The Oleum menthae that adds Herba Artemisiae Annuae extract weight 0.1%.
Repelling effect evaluation methodology: with top embodiment 18.
Experimental result:
The Effective Vate of Protection of contrast anophelifuge mosquito repellent product 1 is 60%, and the Effective Vate of Protection of experiment mosquito repellent product is 98%, and the Effective Vate of Protection of contrast mosquito repellent product 2 is 62%.
As seen, the repelling effect of the supercritical extract take Oleum menthae as entrainer is not significantly better than common common Herba Artemisiae Annuae supercritical extract (using Oleum menthae to be entrainer), also obviously well directly in the common Herba Artemisiae Annuae extract with Oleum menthae.
Embodiment 20: the preparation efficiency comparative experiments of Herba Artemisiae Annuae extract
Equipment: 1L SFE CO
2The supercritical extraction instrument
1) do not add the test of Oleum menthae:
The 5KG Artemisia annua is pulverized as coarse powder (24 orders-65 order), carries out supercritical CO
2Extraction, extracting pressure is 30MPa, and extraction temperature is 60 ℃, and parsing pressure is 8MPa, and resolution temperature is 65 ℃, extraction time is 2 hours.Make Herba Artemisiae Annuae extract 20g.
2) add the Oleum menthae test:
The 5KG Artemisia annua is pulverized and to be coarse powder (24 orders-65 order), take the Oleum menthae of medical material amount 1% as entrainer, carries out supercritical CO
2Extraction, extracting pressure is 30MPa, and extraction temperature is 60 ℃, and parsing pressure is 8MPa, and resolution temperature is 65 ℃, extraction time is 2 hours.Make Herba Artemisiae Annuae extract 80g.
As seen, after adding Oleum menthae, the preparation efficiency of Herba Artemisiae Annuae extract obviously improves.
Although the specific embodiment of the present invention has obtained detailed description, it will be understood to those of skill in the art that.According to disclosed all instructions, can carry out various modifications and replacement to those details, these change all within protection scope of the present invention.Four corner of the present invention is provided by claims and any equivalent thereof.
Claims (15)
1. the preparation method of a Herba Artemisiae Annuae extract, comprise the use supercritical CO
2The step of extraction, wherein, entrainer used is Oleum menthae.
2. preparation method according to claim 1, wherein, the consumption of described Oleum menthae is 0.1%-30%, 0.2%-20%, 0.5%-10 or the 1%-5% of Herba Artemisiae Annuae weight.
3. preparation method according to claim 1 and 2 is characterized in that any one in following (1)-(6) or multinomial:
(1) extracting pressure is 15-35MPa;
(2) extraction temperature is 40 ℃-70 ℃;
(3) extraction time is 1-5 hour;
(4) resolving pressure is 5-10MPa;
(5) resolution temperature is 40 ℃-70 ℃.
4. the described preparation method of any one according to claim 1-3, wherein, Herba Artemisiae Annuae used is through pulverizing.
5. preparation method according to claim 4, wherein, the mean diameter after Herba Artemisiae Annuae is pulverized is 10-80 order, 24-65 order or 24-50 order.
6. the Herba Artemisiae Annuae extract that according to claim 1 to 5, the described preparation method of any one makes.
7. compositions, it comprises Herba Artemisiae Annuae extract claimed in claim 6; Alternatively, described compositions also comprises pharmaceutically or the upper acceptable carrier of physiology or adjuvant.
8. the anophelifuge daily chemical products or the anophelifuge that comprise Herba Artemisiae Annuae extract claimed in claim 6, for example bath foam, cosmetic water, handwashing liquid, fancy soap, perfume or mosquito-repellent water.
9. anophelifuge daily chemical products according to claim 8 or anophelifuge, wherein, the content of described Herba Artemisiae Annuae extract is 0.1%-10% (w/w).
10. according to claim 8 or 9 described anophelifuge daily chemical products or anophelifuges, its component and as follows according to the content of weight percent meter:
The auxiliary surfactant 0%-8% of first type surface activating agent 10%-20%, suds booster 2%-5%, pH adjusting agent is considered, viscosity modifier 0%-0.5%, pearling agent 0%-2%, stabilizing agent 0.05%-1%, essence 0-2% adds water to 100%.
11. anophelifuge daily chemical products according to claim 10 or anophelifuge, wherein,
described first type surface activating agent is selected from: fatty alcohol sulfate sodium, the fatty alcohol sulfate ammonium, the fatty alcohol sulfate triethanolamine, polyoxyethylene alkyl ether sulfate sodium, polyoxyethylene alkyl ether sulfate, polyoxyethylene alkyl ether sulfate magnesium, the polyoxyethylene alkyl ether sulfate triethanolamine, sulfosuccinate sodium, the sulfosuccinate ammonium, the sulfosuccinate triethanolamine, sarcosinate sodium, the sarcosinate ammonium, the sarcosinate triethanolamine, N-acyl group taurine, sodium isethionate, the hydroxyethylsulfonic acid. ammonium, with the hydroxyethylsulfonic acid. triethanolamine,
Described auxiliary surfactant is selected from: betanin, cocos nucifera oil both sexes sodium propionate and glucoside derivative;
Described suds booster is selected from: alkylolamides;
Described pH adjusting agent is selected from: citric acid;
Described viscosity modifier is selected from: the two stearates of PEG-6000, PEG-50 polypropylene glycol oleate, polyacrylic resin, cellulose ether, alkylolamides and HANSHENGJIAO;
Described pearling agent is selected from: ethylene glycol monostearate, Ethylene Glycol Distearate and T
iO
2Cover pigments, mica;
Described stabilizing agent is selected from: parabens, imidazolidinyl urea, dehydroacetic acid and sorbic acid;
Described essence is selected from: Fructus Citri Limoniae essence, Mint Essence, Fructus Citri tangerinae essence and strawberry essence.
12. anophelifuge daily chemical products according to claim 11 or anophelifuge is characterized in that any one or multinomial in the item of following (1)-(4):
(1) described first type surface activating agent is Dodecyl Polyoxyethylene Ether ammonium sulfate and fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium;
(2) described auxiliary surfactant is cocamido propyl betaine;
(3) the described suds booster viscosity modifier of holding concurrently is the fatty acid distribution of coconut oil diglycollic amide;
(4) described pH adjusting agent is citric acid.
13. the purposes of the described Herba Artemisiae Annuae extract of any one in preparation anophelifuge daily chemical products or anophelifuge in claim 1 to 5.
14. Oleum menthae is as supercritical CO
2The purposes of the entrainer in extraction preparation Herba Artemisiae Annuae extract.
15. the method for an anophelifuge or prevention mosquito bite comprises in the claim 1 to 5 of using effective dose the step of the described anophelifuge daily chemical products of any one in the described Herba Artemisiae Annuae extract of any one, compositions claimed in claim 7 or claim 8 to 12 or anophelifuge.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110333657.6A CN103083206B (en) | 2011-10-28 | 2011-10-28 | Sweet wormwood herb extract, preparation method and uses thereof |
| HK13108921.2A HK1181646A1 (en) | 2011-10-28 | 2013-07-30 | Extract of herba artemisiae annuae, method for preparing the same and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201110333657.6A CN103083206B (en) | 2011-10-28 | 2011-10-28 | Sweet wormwood herb extract, preparation method and uses thereof |
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| CN103083206A true CN103083206A (en) | 2013-05-08 |
| CN103083206B CN103083206B (en) | 2015-06-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201110333657.6A Active CN103083206B (en) | 2011-10-28 | 2011-10-28 | Sweet wormwood herb extract, preparation method and uses thereof |
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| CN (1) | CN103083206B (en) |
| HK (1) | HK1181646A1 (en) |
Cited By (6)
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| CN107858220A (en) * | 2017-11-20 | 2018-03-30 | 江门市蓬江区新科美日用工艺品厂有限公司 | A kind of perfumed soap and preparation method thereof |
| CN107929132A (en) * | 2017-12-08 | 2018-04-20 | 蒋琴 | The preparation method of sweet wormwood powder |
| CN108619048A (en) * | 2018-06-06 | 2018-10-09 | 钱龙风 | A kind of except mite mosquito-expelling bath lotion |
| CN108992388A (en) * | 2018-10-24 | 2018-12-14 | 黎桂连 | A kind of hand cleanser of expelling insect and relieving itching and preparation method thereof |
| CN109609267A (en) * | 2019-01-10 | 2019-04-12 | 德利宝(广州)香料有限公司 | A kind of preparation method of Artemisinin essence and dentifrice composition containing the essence |
| CN110623002A (en) * | 2019-09-18 | 2019-12-31 | 禹州市天源生物科技有限公司 | Preparation method of sweet wormwood wax oil insect repellent |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858220A (en) * | 2017-11-20 | 2018-03-30 | 江门市蓬江区新科美日用工艺品厂有限公司 | A kind of perfumed soap and preparation method thereof |
| CN107929132A (en) * | 2017-12-08 | 2018-04-20 | 蒋琴 | The preparation method of sweet wormwood powder |
| CN108619048A (en) * | 2018-06-06 | 2018-10-09 | 钱龙风 | A kind of except mite mosquito-expelling bath lotion |
| CN108992388A (en) * | 2018-10-24 | 2018-12-14 | 黎桂连 | A kind of hand cleanser of expelling insect and relieving itching and preparation method thereof |
| CN109609267A (en) * | 2019-01-10 | 2019-04-12 | 德利宝(广州)香料有限公司 | A kind of preparation method of Artemisinin essence and dentifrice composition containing the essence |
| CN110623002A (en) * | 2019-09-18 | 2019-12-31 | 禹州市天源生物科技有限公司 | Preparation method of sweet wormwood wax oil insect repellent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103083206B (en) | 2015-06-10 |
| HK1181646A1 (en) | 2013-11-15 |
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