Summary of the invention
The present invention is directed to deficiency of the prior art, a kind of trees dormancy activator of breaking plant dormancy and preparation method thereof is provided.
In order to solve the problems of the technologies described above, the present invention is solved by following technical proposals.
A kind of trees dormancy activator, contain the component of following percentage by weight:
6-benzyl aminoadenine 0.001-0.01%;
Gibberellin 0.001-0.02%;
Thiocarbamide 1.0-2.0%;
Zinc sulphate 1.0-3.0%;
Potassium nitrate 2.0-5.0%;
Surfactant 0.1-1.0%;
95% ethanol 0.1-1.5%;
Distilled water surplus.
6-benzyl aminoadenine (6-BA) is a kind of basic element of cell division, can promote nucleic acid, protein to synthesize, and accelerates cell division, promotes the lateral bud growth, promotes chlorophyll to synthesize, and the induced dormancy blastogenesis is long.
Gibberellin (GA3) can synthesize by regulating plant tumor growth element on the one hand, can promote that on the other hand bound form growth hormone is released to free type growth hormone, breaks vegetable nutritorium's dormancy, and Promoting plant growth is grown.
Thiocarbamide (NH
2)
2CS is organic matter-sulphide, can change endogenous hormones and the distribution of carbohydrate in plant corpus in the plant corpus, and it is active to improve POD, helps to break the plant corpus resting state.
The Zn of zinc sulphate
2+Being the component of tryptophan synthetase in the plant corpus, is multiple dehydrase, kinase whose activator, and the content of zinc in plant corpus is lower, replenishes an amount of zinc, can accelerate the physiological metabolism activity of plant.
The K of potassium nitrate
+Be the activator of plurality of enzymes in the plant corpus, can effectively regulate carbohydrate, proteometabolism and respiration; Participate in energy metabolism and matter transportation, Promoting plant growth.
As preferably, surfactant is Tween-20, and chemical name is polyoxyethylene 20 sorbitan monolaurate.The polysorbate class is a large class non-ionic surface active agent, has emulsification, diffusion, solubilising, the effect such as stable.
The preparation method of trees dormancy activator may further comprise the steps:
The first step takes by weighing each component in proportion, respectively thiocarbamide, zinc sulphate, potassium nitrate is added an amount of distilled water and fully dissolves, and is for subsequent use;
Second step is got 6-benzyl aminoadenine, and with a little 95% ethanol, fully after the dissolving, adding distil water is prepared 0.1% 6-benzyl aminoadenine solution, and is for subsequent use;
The 3rd step, get gibberellin, with a little 95% ethanol, fully after the dissolving, adding distil water is prepared 0.2% Gibberellins solution, and storage temperature is that 4 ℃ (putting in the cold compartment of refrigerator) are for subsequent use;
The 4th step with above solution for subsequent use, mixed successively, added surfactant and fully stirred, and added remaining distilled water;
In the 5th step, the solution sterilization that the 4th step made is made trees dormancy activator, the tinning sealing.
The invention provides a kind of trees dormancy activator, with multiple active ingredient with breaking dormancy, by the proper proportion preparation, additive effect by each constituent, and reach the Endogenous Hormone Contents in Vitro of short dormancy in the tree body is reduced, the endogenous hormones that promotes physiological metabolism to accelerate raises, and in the short period of time, makes trees change the effect of active growth state over to from resting state.Has broad spectrum activity, characteristics simple for use.Spray during use in the tree surface and get final product.
Embodiment
Below by embodiment the present invention is described in further detail.
Embodiment 1
A kind of preparation method of trees dormancy activator may further comprise the steps:
The first step takes by weighing each component,
6-benzyl aminoadenine 10mg;
Gibberellin 10mg;
Thiocarbamide 1.0g;
Zinc sulphate 1.0g;
Potassium nitrate 2.0g;
Tween-20 0.1g;
95% ethanol 10ml;
Distilled water 1.0L.
Respectively thiocarbamide, zinc sulphate, potassium nitrate are added 100ml distilled water and fully dissolve, for subsequent use;
Second step is got 6-benzyl aminoadenine, adds 95% ethanol 5ml, and fully after the dissolving, adding distil water 100ml dilution shakes up, and is for subsequent use;
The 3rd step, get gibberellin, add 95% ethanol 5ml, fully after the dissolving, adding distil water 100ml dilution shakes up, and storage temperature is that 4 ℃ (putting in the cold compartment of refrigerator) are for subsequent use;
The 4th step with above solution for subsequent use, mixed successively, added surfactant and fully stirred, and added remaining distilled water;
In the 5th step, the solution sterilization that the 4th step made is made trees dormancy activator, the tinning sealing.
Embodiment 2
A kind of preparation method of trees dormancy activator may further comprise the steps:
The first step takes by weighing each component,
6-benzyl aminoadenine 100mg;
Gibberellin 200mg;
Thiocarbamide 2.0g;
Zinc sulphate 3.0g;
Potassium nitrate 5.0g;
Tween-20 1.0g;
95% ethanol 10ml;
Distilled water 1.0L.
Respectively thiocarbamide, zinc sulphate, potassium nitrate are added 100ml distilled water and fully dissolve, for subsequent use;
Second step is got 6-benzyl aminoadenine, adds 95% ethanol 5ml, and fully after the dissolving, adding distil water 100ml dilution shakes up, and is for subsequent use;
The 3rd step, get gibberellin, add 95% ethanol 5ml, fully after the dissolving, adding distil water 100ml dilution shakes up, and storage temperature is that 4 ℃ (putting in the cold compartment of refrigerator) are for subsequent use;
The 4th step with above solution for subsequent use, mixed successively, added surfactant and fully stirred, and added remaining distilled water;
In the 5th step, the solution sterilization that the 4th step made is made trees dormancy activator, the tinning sealing.
Embodiment 3
A kind of preparation method of trees dormancy activator may further comprise the steps:
The first step takes by weighing each component,
6-benzyl aminoadenine 50mg;
Gibberellin 100mg;
Thiocarbamide 1.5g;
Zinc sulphate 1.5g;
Potassium nitrate 2.5g;
Tween-20 0.5g;
95% ethanol 10ml;
Distilled water 1.0L.
Respectively thiocarbamide, zinc sulphate, potassium nitrate are added 100ml distilled water and fully dissolve, for subsequent use;
Second step is got 6-benzyl aminoadenine, adds 95% ethanol 5ml, and fully after the dissolving, adding distil water 100ml dilution shakes up, and is for subsequent use;
The 3rd step, get gibberellin, add 95% ethanol 5ml, fully after the dissolving, adding distil water 100ml dilution shakes up, and storage temperature is that 4 ℃ (putting in the cold compartment of refrigerator) are for subsequent use;
The 4th step with above solution for subsequent use, mixed successively, added surfactant and fully stirred, and added remaining distilled water;
In the 5th step, the solution sterilization that the 4th step made is made trees dormancy activator, the tinning sealing.
Embodiment 4
On February 27th, 2012, base, safe nursery in Hangzhou, respectively 120 strains were as subjects with 5 years fragrant camphor trees of life, sweet osmanthus, beech trees, and observation sprays example 1, example 2 and example 3 and obtains trees dormancy activator to the impact of germination rate.One week is rear with the test stone of rudiment number as the action effect of trees dormancy activator.
Experimental group: choose 3 years fragrant camphor trees of life, sweet osmanthus, each 90 strain of beech tree, 30 is one group, and first group sprays example 1 and make trees dormancy activator, and second group sprays example 2 and make trees dormancy activator, and the 3rd group sprays example 3 and make trees dormancy activator.
Control group: choose 30 fragrant camphor trees of life of residue, sweet osmanthus, each 30 strain of beech tree, spray clear water.
Result of the test sees the following form:
Seeds |
First group of germination rate % |
Second group of germination rate |
The 3rd group of germination rate |
Contrast germination rate % |
Fragrant camphor tree |
70.7 |
60.3 |
74.8 |
51.3 |
Sweet osmanthus |
67.3 |
72.2 |
81.6 |
43.4 |
The beech tree |
80.6 |
78.1 |
86.2 |
46.1 |
Conclusion: spray germination rate behind the trees dormancy activator apparently higher than the germination rate that sprays behind the clear water, trees dormancy activator can be broken plant dormancy, promotes rudiment.
Embodiment 5
On February 27th, 2012, base, safe nursery in Zhejiang Hangzhou Yuhang, with life in 3 years and 15 years living sweet osmanthus, life in 3 years and each 20 strain of 10 years living beech trees are as subjects, spray example 3 and make trees dormancy activator, two weeks are rear with the test stone of rudiment number as the action effect of trees dormancy activator.
Experimental group: choose life in 3 years and 15 years living sweet osmanthus, each 10 strain of beech tree, spray example 3 and make trees dormancy activator;
Control group: choose residue 3 years living sweet osmanthus, beech and set each 10 strain, spray clear water.
Result of the test sees the following form:
Seeds |
Spray rear germination rate % |
Contrast germination rate % |
3 years living sweet osmanthus |
87.6 |
51.2 |
15 years living sweet osmanthus |
71.4 |
34.2 |
3 years living beech trees |
86.2 |
46.1 |
10 years living beech trees |
72.3 |
39.7 |
Conclusion: for earsh and strong age tree, spray germination rate behind the trees dormancy activator apparently higher than the germination rate that sprays behind the clear water, trees dormancy activator can be broken plant dormancy, promotes rudiment.
The above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to the covering scope of patent of the present invention.