CN106386346A - Bischofia javanica rapid reproducing method - Google Patents
Bischofia javanica rapid reproducing method Download PDFInfo
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- CN106386346A CN106386346A CN201610795522.4A CN201610795522A CN106386346A CN 106386346 A CN106386346 A CN 106386346A CN 201610795522 A CN201610795522 A CN 201610795522A CN 106386346 A CN106386346 A CN 106386346A
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- ramulus
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- bischofiae javanicae
- folium bischofiae
- beta pruning
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention relates to a Bischofia javanica rapid reproducing method. The Bischofia javanica rapid reproducing method includes: adopting a plant tissue culture principle to prepare cultivation soil, a nutriment solution, a pretreatment solution, and a rooting solution; completely watering the cultivation soil with the nutriment solution; using semi-lignified twigs of Bischofia javanica as reproducing materials, cutting the semi-lignified twigs into stem segments with buds; treating the stem segments with the pretreatment solution, and then inserting the stem segments into the cultivation soil; spraying the stem segments with the rooting solution after a week so as to promote rooting, performing spraying once every week till the stem segments take roots, wherein the new plants can be obtained through cultivation and management for 3-4 weeks, the survival rate can reach more than 90%, and the root systems are developed. The advantages of the invention are that the Bischofia javanica rapid reproducing method is simple and easy to operate, is economic in materials which can be acquired easily, is easy to master, is easy to promote, can achieve industrialized seedling production, and can solve the technical problems of Bischofia javanica rapid reproduction well.
Description
Technical field
The present invention relates under a kind of condition of tissue culture Ramulus et Folium Bischofiae Javanicae culture, belong to plant technology field.
Background technology
Ramulus et Folium Bischofiae Javanicae, Euphorbiaceae, evergreen or half aiphyllium, is often born in the spacious ground of low altitude area, especially with river bank embankment, moistens
Fertile sand loam is most widely suited.Distribution Fujian, Guangdong, Guangxi, Shaanxi, Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Hunan,
Hubei, Sichuan, Guizhou, Yunnan, the ground such as Taiwan.Root contains β-Amyrin, ursolic acid, cupreol.Sarcocarp can be made wine.Seed contains
Oil mass 30-54%, edible, also can make lubricating oil.Bark contains epifriedelanol acetate, freidelin, cupreol and Betula platyphylla Suk. fat
Sour methyl ester.Stem contains freidelin, friedelan 3 βol.Branch and leaf contain saccharide, aminoacid, phenolic constituent, flavonoid, Coumarinses.Leaf
Still contain acid potassium tartrate and Calcium d-tartrate 8-16%, acetic acid freidelin -3 α -ester, freidelin, friedelan 3 βol, freidelin 3
α -ol, cupreol, gallic acid.Bark can extract orchil.Leaf can make green manure, also can control innominate toxic swelling.Root has wind-dispelling
Detumescence effect, cures mainly rheumatic ostalgia, dysentery etc..Current modes of reproduction is still natural propagation, and resource utilization is low, tissue culture method
Large-scale production Ramulus et Folium Bischofiae Javanicae seedling can be reached so as to resource obtains reasonable development.
Content of the invention
The technical problem to be solved is to provide a kind of Ramulus et Folium Bischofiae Javanicae rapid propagation method, easy to operation,
Survival rate is high, and growth cycle is short, can obtain substantial amounts of Ramulus et Folium Bischofiae Javanicae seedling in a short time, contribute to the effectively utilizes of Ramulus et Folium Bischofiae Javanicae.
The purpose of the present invention is achieved through the following technical solutions:
A kind of autumn maple method for quickly breeding, comprises the following steps:
(1) take the stem section of Ramulus et Folium Bischofiae Javanicae, Ramulus et Folium Bischofiae Javanicae semi-lignified spray is cut into segment;
(2) configure compost, and irrigate compost with nutritional solution;And will be equipped with the container of described compost and be placed in screening
Sun, the place of ventilation;
(3) to Ramulus et Folium Bischofiae Javanicae stem section disinfection;
(4) Ramulus et Folium Bischofiae Javanicae stem section is carried out with pretreatment, described preprocessing process is:Upper pretreatment is dipped in Ramulus et Folium Bischofiae Javanicae beta pruning bottom
After liquid, its underpart is inserted in compost;
(5) taking root liquid is sprayed in the Ramulus et Folium Bischofiae Javanicae beta pruning of cuttage, spraying for the first time is in described Ramulus et Folium Bischofiae Javanicae beta pruning cuttage
Carry out after one week, spray weekly once later, until take root surviving;The same period carries out diseases prevention, mould proof, insect protected behaviour to described beta pruning
Make;
(6) after the Ramulus et Folium Bischofiae Javanicae beta pruning of cuttage is taken root and survived, topdressed, the same period prevents to described Ramulus et Folium Bischofiae Javanicae beta pruning
Sick, mould proof, insect protected operation;
(7), when allowing Ramulus et Folium Bischofiae Javanicae beta pruning grow to 15~40cm height in compost, carry out carrying soil field planting.
Specifically, described in step (1), the every segment of Ramulus et Folium Bischofiae Javanicae beta pruning has 2~12 true leaves, at least one terminal bud or lateral bud,
Ensure that beta pruning has enough proliferation activities.
Specifically, compost described in step (2) is one of following compost;
The compost that yellow soil is mixed homogeneously by volume with wood sawdust, yellow soil:Wood sawdust=3:1~2;
Or, the compost that yellow soil is mixed homogeneously by volume with fertile soil, yellow soil:Fertile soil=3:1~2;
Or, the compost that yellow soil is mixed homogeneously by volume with river sand, yellow soil:River sand=3:1~2;
Or, the compost that yellow soil is mixed homogeneously by volume with bagasse, yellow soil:Bagasse=3:1~2.
Compost according to above proportioning components mix homogeneously all can provide environment and the base needed for proliferation for beta pruning
Plinth nutrient, and material is economical and easily available, cost performance is high.
Specifically, described in step (2), the component proportion of nutritional solution is:Constituent content in every liter of nutritional solution is respectively
For:The ammonium nitrate of 410mg, the potassium nitrate of 475mg, the potassium dihydrogen phosphate of 42.5mg, the CALCIUM CHLORIDE DIHYDRATE of 110mg, 92.5mg
Bitter salt, the boric acid of 1.5mg, the Zinc vitriol of 2.1mg, the two molybdic acid hydrate sodium of 0.08mg, the seven of 14mg
Ferrous sulfate hydrate, the heteroauxing of 0.18mg, the carbamide of 48mg, balance of water.
The present invention uses the nutritional solution of individual components proportioning, can supplement the nutrients for beta pruning rapidly comprehensively, fast by plant cell
Speed absorb, can than existing nutritional solution more comprehensively, more fully supplement arboreal growth required for nutrition, more thoroughly excite and cut
The activity of branch cell, promotes beta pruning plant cell differentiation and division, can make the beta pruning of culture fast quick-recovery within the shorter time
The growth impetus, improves the survival rate of culture beta pruning.
Preferably, add the Polyvinylpyrrolidone that quality accounting is 1% in the compost described in step (2), permissible
Prevent from occurring browning in incubation.
Specifically, Ramulus et Folium Bischofiae Javanicae stem with bud described in step (3) disinfect for:Take Ramulus et Folium Bischofiae Javanicae stem with bud, in drift
Soak 3 minutes in white lead, wire tap water rinses 35~45 minutes, 70% ethanol postincubation 30 seconds, mercuric chloride process 15 minutes,
Use aseptic water washing 5~8 times afterwards.
Specifically, the pretreatment fluid described in step (4) is by indolebutyric acid and naphthalene acetic acid in mass ratio 3:2 are added to the water
The mixed solution of configuration, total soluble matters concentration 100~1000ppm;Can effectively facilitate at beta pruning wound the division of cell and point
Change, promote beta pruning wound to recover regeneration capacity.
Specifically, the component of taking root liquid described in step (5) and weight are:
Using the distinctive component formula of the present invention, beta pruning is sprayed with this taking root liquid, its active ingredient and nutrients help
Absorbed by beta pruning rapidly, composition and the battalion required for beta pruning is taken root can be supplemented than existing taking root liquid more comprehensively, more quickly
Support, promote plant cell differentiation and division, promote the new wound of new root and root system Rapid development, beta pruning can be made fast within the shorter time
Quick-recovery rootability.
Specifically, described in step (6), the key component of fertilizer topdressed and content are:0.3% carbamide and 0.3%
Ferrous sulfate be composited or 0.3% potassium dihydrogen phosphate with 0.1% many spirit bacterium be composited.
Compared with prior art, the present invention has advantages below:Born with the Ramulus et Folium Bischofiae Javanicae nursery stock 20~30 that the present invention produces
Root, well developed root system, survival rate more than 90%;Operation is simple for this method for culturing seedlings, and material is economical and easily available, can factorial praluction,
Efficiently solve Ramulus et Folium Bischofiae Javanicae resource supply and demand contradiction, and easy to spread.The autumn maple method for quickly breeding providing by using the present invention,
Can be rapidly that the beta pruning being cultured supplements required composition and nutrition, excite the activity of beta pruning, promote the quick restoration ecosystem of beta pruning
The impetus, improves the survival rate of the beta pruning of tissue culture, and effect projects, and will not have prominent to environment after using
The substantive distinguishing features that go out and significantly improving.
Specific embodiment
For the ease of understanding the present invention, the present invention will be described more fully below, give the preferable of the present invention
Embodiment.But, the present invention can realize in many different forms however it is not limited to embodiments described herein.
On the contrary, provide the purpose of these embodiments be make to the disclosure understand more thoroughly comprehensive.
Unless otherwise defined, all of technology used herein and scientific terminology and the technical field belonging to the present invention
The implication that technical staff is generally understood that is identical.The term being used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body is it is not intended that in limiting the present invention.Term as used herein " and/or " include one or more
The arbitrary and all of combination of related Listed Items.
With reference to embodiment, the invention will be further described:
Embodiment 1
(1) take the stem section of Ramulus et Folium Bischofiae Javanicae, Ramulus et Folium Bischofiae Javanicae semi-lignified spray is cut into segment, every segment has 2~12 true leaves, extremely
A few terminal bud or lateral bud;
(2) yellow soil and fertile soil are used with volume ratio 3:2 ratio mix homogeneously configuration compost, and irrigated with nutritional solution
Compost;Then toward attaching polyethylene ketopyrrolidine in compost, and will be equipped with the container of the described compost having configured and put
Place in sunshade, ventilation;
Constituent content in every liter of nutritional solution is respectively:The ammonium nitrate of 410mg, the potassium nitrate of 475mg, the phosphoric acid of 42.5mg
Potassium dihydrogen, the CALCIUM CHLORIDE DIHYDRATE of 110mg, the bitter salt of 92.5mg, the boric acid of 1.5mg, seven hydrated sulfuric acid of 2.1mg
Zinc, the two molybdic acid hydrate sodium of 0.08mg, the green vitriol of 14mg, the heteroauxing of 0.18mg, the carbamide of 48mg, surplus
For water;
(3) to Ramulus et Folium Bischofiae Javanicae stem section disinfection:Take Ramulus et Folium Bischofiae Javanicae stem with bud, soak 3 minutes in bleaching powder, wire
Tap water rinses 40 minutes, 70% ethanol postincubation 30 seconds, and mercuric chloride is processed 15 minutes, finally uses aseptic water washing 5~8 times;
(4) Ramulus et Folium Bischofiae Javanicae stem section is carried out with pretreatment, described preprocessing process is:Upper pretreatment is dipped in Ramulus et Folium Bischofiae Javanicae beta pruning bottom
After liquid, in its underpart insertion compost;Described pretreatment fluid is by indolebutyric acid and naphthalene acetic acid in mass ratio 3:2 are added to the water configuration
Mixed solution, total soluble matters concentration 400ppm;
(5) taking root liquid is sprayed in the beta pruning of cuttage, spraying for the first time is to carry out after one week in described beta pruning cuttage, with
Spray weekly once afterwards, until take root surviving;The same period carries out diseases prevention, mould proof, insect protected operation to described beta pruning;Described taking root liquid
Component and weight be:
(6) after the beta pruning of cuttage is taken root and survived, topdressed, the same period carries out diseases prevention, mould proof, insect protected behaviour to described beta pruning
Make;The key component of the described fertilizer topdressing and carbamide that content is 0.3% and 0.3% ferrous sulfate are composited.
(7), when allowing beta pruning grow to 15~40cm height in compost, carry out carrying soil field planting.
Embodiment 2
(1) take the stem section of Ramulus et Folium Bischofiae Javanicae, Ramulus et Folium Bischofiae Javanicae semi-lignified spray is cut into segment, every segment has 2~12 true leaves, extremely
A few terminal bud or lateral bud;
(2) yellow soil and wood sawdust are used with volume ratio 3:1 ratio mix homogeneously configuration compost, and irrigated with nutritional solution
Compost, then toward attaching polyethylene ketopyrrolidine in compost, and will be equipped with the container of the described compost having configured and puts
Place in sunshade, ventilation;
Constituent content in every liter of nutritional solution is respectively:The ammonium nitrate of 410mg, the potassium nitrate of 475mg, the phosphoric acid of 42.5mg
Potassium dihydrogen, the CALCIUM CHLORIDE DIHYDRATE of 110mg, the bitter salt of 92.5mg, the boric acid of 1.5mg, seven hydrated sulfuric acid of 2.1mg
Zinc, the two molybdic acid hydrate sodium of 0.08mg, the green vitriol of 14mg, the heteroauxing of 0.18mg, the carbamide of 48mg, surplus
For water;
(3) to Ramulus et Folium Bischofiae Javanicae stem section disinfection:Take Ramulus et Folium Bischofiae Javanicae stem with bud, soak 3 minutes in bleaching powder, wire
Tap water rinses 40 minutes, 70% ethanol postincubation 30 seconds, and mercuric chloride is processed 15 minutes, finally uses aseptic water washing 5~8 times;
(4) Ramulus et Folium Bischofiae Javanicae stem section is carried out with pretreatment, described preprocessing process is:Upper pretreatment is dipped in Ramulus et Folium Bischofiae Javanicae beta pruning bottom
After liquid, in its underpart insertion compost;Described pretreatment fluid is by indolebutyric acid and naphthalene acetic acid in mass ratio 3:2 are added to the water configuration
Mixed solution, total soluble matters concentration 600ppm;
(5) taking root liquid is sprayed in the beta pruning of cuttage, spraying for the first time is to carry out after one week in described beta pruning cuttage, with
Spray weekly once afterwards, until take root surviving;The same period carries out diseases prevention, mould proof, insect protected operation to described beta pruning;Described taking root liquid
Component and weight be:
(6) after the beta pruning of cuttage is taken root and survived, topdressed, the same period carries out diseases prevention, mould proof, insect protected behaviour to described beta pruning
Make;The key component of the described fertilizer topdressing and potassium dihydrogen phosphate that content is 0.3% and 0.1% many spirit bacterium be combined and
Become.
(7), when allowing beta pruning grow to 15~40cm height in compost, carry out carrying soil field planting.
The above embodiment only have expressed the several embodiments of the present invention, and its description is more concrete and detailed, but
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that the ordinary skill people for this area
For member, without departing from the inventive concept of the premise, some deformation can also be made and improve, these broadly fall into the present invention's
Protection domain.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (9)
1. a kind of autumn maple method for quickly breeding is it is characterised in that comprise the following steps:
(1) take the stem section of Ramulus et Folium Bischofiae Javanicae, Ramulus et Folium Bischofiae Javanicae semi-lignified spray is cut into segment;
(2) configure compost, and irrigate compost with nutritional solution;And will be equipped with the container of described compost and be placed in sunshade, logical
The place of wind;
(3) to Ramulus et Folium Bischofiae Javanicae stem section disinfection;
(4) Ramulus et Folium Bischofiae Javanicae stem section is carried out with pretreatment, described preprocessing process is:Upper pretreatment fluid is dipped in Ramulus et Folium Bischofiae Javanicae beta pruning bottom
Afterwards, its underpart is inserted in compost;
(5) taking root liquid is sprayed in the Ramulus et Folium Bischofiae Javanicae beta pruning of cuttage, spraying for the first time is in described Ramulus et Folium Bischofiae Javanicae beta pruning cuttage one week
After carry out, spray weekly once, until taking root and surviving later;The same period carries out diseases prevention, mould proof, insect protected operation to described beta pruning;
(6) after the Ramulus et Folium Bischofiae Javanicae beta pruning of cuttage is taken root and survived, topdressed, the same period carries out diseases prevention, prevents to described Ramulus et Folium Bischofiae Javanicae beta pruning
Mould, insect protected operation;
(7), when allowing Ramulus et Folium Bischofiae Javanicae beta pruning grow to 15~40cm height in compost, carry out carrying soil field planting.
2. autumn maple method for quickly breeding according to claim 1 is it is characterised in that Ramulus et Folium Bischofiae Javanicae beta pruning described in step (1)
Every segment has 2~12 true leaves, at least one terminal bud or lateral bud.
3. autumn maple method for quickly breeding according to claim 1 is it is characterised in that under described in step (2), compost is
One of row compost;
The compost that yellow soil is mixed homogeneously by volume with wood sawdust, yellow soil:Wood sawdust=3:1~2;
Or, the compost that yellow soil is mixed homogeneously by volume with fertile soil, yellow soil:Fertile soil=3:1~2;
Or, the compost that yellow soil is mixed homogeneously by volume with river sand, yellow soil:River sand=3:1~2;
Or, the compost that yellow soil is mixed homogeneously by volume with bagasse, yellow soil:Bagasse=3:1~2.
4. autumn maple method for quickly breeding according to claim 1 is it is characterised in that the group of nutritional solution described in step (2)
Distribution ratio is:Constituent content in every liter of nutritional solution is respectively:The ammonium nitrate of 410mg, the potassium nitrate of 475mg, 42.5mg's
Potassium dihydrogen phosphate, the CALCIUM CHLORIDE DIHYDRATE of 110mg, the bitter salt of 92.5mg, the boric acid of 1.5mg, seven hydrations of 2.1mg
Zinc sulfate, the two molybdic acid hydrate sodium of 0.08mg, the green vitriol of 14mg, the heteroauxing of 0.18mg, the carbamide of 48mg,
Balance of water.
5. autumn maple method for quickly breeding according to claim 1 is it is characterised in that in the compost described in step (2)
Add the Polyvinylpyrrolidone that quality accounting is 1%.
6. autumn maple method for quickly breeding according to claim 1 is it is characterised in that Ramulus et Folium Bischofiae Javanicae band bud described in step (3)
Stem section disinfect for:Take Ramulus et Folium Bischofiae Javanicae stem with bud, soak 3 minutes in bleaching powder, wire tap water rinses 35~45 points
Clock, 70% ethanol postincubation 30 seconds, mercuric chloride is processed 15 minutes, finally uses aseptic water washing 5~8 times.
7. autumn maple method for quickly breeding according to claim 1 is it is characterised in that the pretreatment fluid described in step (4)
It is by indolebutyric acid and naphthalene acetic acid in mass ratio 3:2 mixed solutions being added to the water configuration, total soluble matters concentration 100~
1000ppm.
8. autumn maple method for quickly breeding according to claim 1 is it is characterised in that the taking root liquid described in step (5)
Component and weight are:
9. autumn maple method for quickly breeding according to claim 1 is it is characterised in that the fertilizer that topdresses described in step (6)
The key component of material and content are:0.3% carbamide and 0.3% ferrous sulfate be composited or 0.3% potassium dihydrogen phosphate
It is composited with 0.1% many spirit bacterium.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107306743A (en) * | 2017-08-02 | 2017-11-03 | 合肥雪立歆农业科技有限公司 | A kind of big walnut tree planting method for transplanting |
CN107836264A (en) * | 2017-10-19 | 2018-03-27 | 界首市金玖江农业发展有限公司 | A kind of method of mountain area plantation Chinese bandoline wood |
CN110432151A (en) * | 2019-09-11 | 2019-11-12 | 安徽农业大学 | A kind of tissue culture and rapid propagation method of Bischofia javanica Bl |
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CN102726202A (en) * | 2012-07-19 | 2012-10-17 | 湖南省森林植物园 | Method for rapidly propagating taxus chinensis seedlings |
CN104255455A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Method for quickly reproducing bischofia javanica |
CN104429807A (en) * | 2014-12-10 | 2015-03-25 | 江苏省林业科学研究院 | Transplanting method of mature large trees |
CN104663210A (en) * | 2015-02-14 | 2015-06-03 | 河北省林业科学研究院 | Elm cutting propagation method |
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Patent Citations (4)
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CN102726202A (en) * | 2012-07-19 | 2012-10-17 | 湖南省森林植物园 | Method for rapidly propagating taxus chinensis seedlings |
CN104255455A (en) * | 2014-09-12 | 2015-01-07 | 南京通泽农业科技有限公司 | Method for quickly reproducing bischofia javanica |
CN104429807A (en) * | 2014-12-10 | 2015-03-25 | 江苏省林业科学研究院 | Transplanting method of mature large trees |
CN104663210A (en) * | 2015-02-14 | 2015-06-03 | 河北省林业科学研究院 | Elm cutting propagation method |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107306743A (en) * | 2017-08-02 | 2017-11-03 | 合肥雪立歆农业科技有限公司 | A kind of big walnut tree planting method for transplanting |
CN107836264A (en) * | 2017-10-19 | 2018-03-27 | 界首市金玖江农业发展有限公司 | A kind of method of mountain area plantation Chinese bandoline wood |
CN110432151A (en) * | 2019-09-11 | 2019-11-12 | 安徽农业大学 | A kind of tissue culture and rapid propagation method of Bischofia javanica Bl |
CN110432151B (en) * | 2019-09-11 | 2022-03-25 | 安徽农业大学 | Tissue culture and rapid propagation method of Chongyang wood |
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Application publication date: 20170215 |