CN103007111A

CN103007111A - Chinese medicinal preparation for treating diarrhea of rabbits and preparation method of Chinese medicinal preparation

Info

Publication number: CN103007111A
Application number: CN2012105812497A
Authority: CN
Inventors: 杨高林
Original assignee: Qingdao Huaren Information Technology Development Co Ltd
Current assignee: Xinyang Agriculture and Forestry University
Priority date: 2012-12-27
Filing date: 2012-12-27
Publication date: 2013-04-03
Anticipated expiration: 2032-12-27
Also published as: CN103007111B

Abstract

本发明提供一种治疗兔腹泻的中药制剂，所述中药制剂包括：穿心莲、石榴皮、败酱草、地榆、白头翁、当归、白芨、薤白、焦神曲、白术、生地、白花蛇舌草、蒲公英、黄连、秦皮、大腹皮、党参、黄芩、土茯苓和青蒿。本发明针对兔子饲养中易发腹泻的问题，添加去燥湿，健脾胃、补中益气、增强抗病力的中药添加剂；不但能直接杀菌抑菌,而且能调节机体的免疫机能,具有非特异抗菌作用，并且不产生依赖性；还能促进兔生长，摒弃化学抗生素对兔体的伤害，间接影响食品安全的副作用，采用全新的中药添加剂，达到绿色食品纯天然的要求。The invention provides a traditional Chinese medicine preparation for treating diarrhea in rabbits. The traditional Chinese medicine preparation includes: andrographis paniculata, pomegranate peel, Patrinia, Burnet, Pulsatilla, Angelica, Bletilla striata, Scallops, Jiao Shenqu, Atractylodes macrocephala, Shengdi, Hedyotis diffusa, Dandelion, Coptis chinensis, Qinpi, Dabupi, Codonopsis, Scutellaria, Smilax and Artemisia annua. The invention aims at the problem of prone to diarrhea in raising rabbits, and adds traditional Chinese medicine additives to remove dampness, invigorate the spleen and stomach, invigorate the middle and replenish qi, and enhance disease resistance; it can not only directly sterilize and inhibit bacteria, but also regulate the immune function of the body, and has non-toxic It has specific antibacterial effect and does not produce dependence; it can also promote the growth of rabbits, abandon the harm of chemical antibiotics to the rabbit body, and indirectly affect the side effects of food safety. It adopts brand-new traditional Chinese medicine additives to meet the requirements of pure natural green food.

Description

A kind of Chinese medicine preparation for the treatment of rabbit diarrhoea and preparation method thereof

Technical field

The present invention relates to a kind of with animal, the plant Chinese medicine preparation as raw material production, particularly a kind of Chinese medicine preparation for the treatment of rabbit diarrhoea and preparation method thereof.

Background technology

In the breeding process of rabbit, diarrhoea is one of modal disease, and its onset speed is very fast, and pathogenic factor is complicated, is the Etiological that causes the rabbit death of the young north to the Great Wall.Therefore, must prevent the diarrhoea of north to the Great Wall rabbit, accomplish early to find early treatment.

The reason of 1 diarrhoea

North to the Great Wall rabbit diarrhoeal diseases mainly causes by antibacterial, virus, fungus, toxin, parasite and biological paathogenic factor, and the improper digestive disorders that causes of feedstuff and feeding and management also is the modal cause of disease.

1.1 the diarrhoea that dyspepsia causes

1 monthly age, above young north to the Great Wall rabbit was multiple, and main manifestations is for defecation is frequent, loose and watery stool is soft, be medicated porridge sample or watery stool, and spirit is depressed, loss of appetite, become thin gradually, be mixed with blood or gel-shaped mucus in the serious sick rabbit watery diarrhea thing, the conjunctiva cyanosis, rapid breathing is often collapsed and death.

1.2 antibacterial infects the diarrhoea that causes

Acute or the chronic disease that Escherichia coli and toxin thereof cause.Mainly betide the young rabbit of 25-70 age in days, the rabbit of falling ill shows as diarrhoea or constipation, and what have is watery diarrhea, and gel-shaped mucus is often arranged in the feces.During improper or weather drastic change, north to the Great Wall rabbit body resistance descends when feeding and management, and escherichia coli quantity can sharply increase, thereby causes morbidity.The acute infectious disease of the digestive tract that the extracellular toxin of A type clostridieum welchii causes, its sickness rate, mortality rate are all higher.The equal susceptible of the rabbit at each kind, age, wherein the young rabbit invasion rate at 1-3 monthly age is high.The diarrhoea that salmonellosis causes mainly betides young north to the Great Wall rabbit and conceived 25 days later female rabbits.

1.3 the diarrhoea that viral infection causes

Transmissible gastroenteritis is acute infectious intestinal disease.Main manifestations is anorexia and diarrhoea, especially in cold season, can infect rapidly the rabbit at various ages, the dehydration that the normal generation of the young rabbit of 20-30 age in days is serious and electrolyte forfeiture, and case fatality rate is higher.Young rabbit case fatality rate more than 3 monthly ages is lower.The epidemic diarrhea infectious intestinal disease not only betides young rabbit, and at adult rabbits generation is arranged also, and infection rate is 100%, and the M ﹠ M of young north to the Great Wall rabbit and young north to the Great Wall rabbit is all higher.Clinical manifestation is draining sample feces and dehydration, and annual each Ji Junke occurs.

1.4 the diarrhoea that parasitic infection causes

The internal parasitic disease that is caused by multiple coccidiosis.Clinically take become thin, anemia, hemafecia and growth retardation be as feature.Mainly young north to the Great Wall rabbit and the young north to the Great Wall rabbit with the 20-70 age in days infects the most serious.

Diarrhoea rabbit general abdominal part expands, hypourocrinia and be canescence, and gastrointestinal peristalsis strengthens, and has seasonal disease north to the Great Wall rabbit to present stomachache.Sick north to the Great Wall rabbit down in spirits, astasia, weakness, fervescence, disorderly thick by hair, poor appetite or absolutely useless, and diarrhoea appears, begin to be yellow loose stool, later stage is changed taupe or black watery stools into, have just in band blood or open-minded film is arranged, flavor is stench, most of sick north to the Great Wall rabbit hind leg, anus and afterbody are polluted by hair, and indivedual serious anuses stop up.Disease north to the Great Wall rabbit coolness of extremities, eye socket sink, and becomes thin rapidly, death in 2-3 days after the general morbidity, the sustainable 5-7 of some courses of disease days.

Rabbit is plant-eating animal, and the Ying Yicao that feeds is main, and concentrate is auxiliary, suitably mend with coarse fodder, be strictly on guard against that concentrate is too much or crude fiber content is little, this point is easily out in the cold, though crude fibre is little easy to digest, but can fill gastrointestinal tract, stimulating gastrointestinal road mucosa is safeguarded intestinal smooth muscle system tonicity, reduce caecum, colon load is avoided food to form agglomerate in gastrointestinal and is caused that diarrhoea appears in enteritis, and crude fiber content should maintain in the 12-6% scope in the daily ration.Although feeding antibiotic class medicine has greatly promoted the development of aquaculture, and be described as the milestone of animal nutrition in this century, but the abuse of antibiotics problem of spreading out more bad and more bad in recent years, not only affected feeding antibiotic drug effect and livestock products quality, and masses' consumer psychology has been produced negative effect.

Choosing medical herbs is safe feed additive, follow Traditional Chinese Medicine theory and modern animal nutrition principle always, have with food homology, consubstantiality, with the characteristics of usefulness, have the dual function of nutrition and medicine concurrently, its immunity substance mass-energy enhancing human body immunity function, improve animal anti-stress, anti-disease ability, improve breeding performonce fo animals, go back simultaneously toxic side effect little, have no drug resistance, be difficult for the advantages such as residual.Facts have proved, Chinese herbal feed additive is applied to the energy Effective Raise rate of animals delivered to the slaughter-house in the rabbit raising of the north to the Great Wall, and survival rate is improved north to the Great Wall Carnis Leporis mouthfeel, can directly affect ecological benefits, economic benefit and social benefit.

Chinese herbal feed additive, refer to take and take the subjects theory such as raising and feed industry and technology as support single and compound Chinese herbal medicine additive or the intermixture developed as the leading factor with the traditional theory that China concerns the physical property of large right Chinese herbal medicine (yin, yang, tremble with fear, freeze, temperature, heat), thing flavor (sour, sweet, bitter, peppery, salty) and material.

Because many drawbacks of feeding antibiotic class and chemical synthetic drug additive, its dosage of using and kind are all being controlled every year in countries in the world in animal husbandry, and the while is at the feed additive of trying to explore other safety and environmental protection type.Present rabbit meat poor hygienic, domestic standard and international standard disunity, drug residue exceeds standard, and it is keeping in check of restriction China's Carnis Leporis development that quality does not reach importer's requirement.China is main mainly with supporting rabbit on a small scale, abuses medicine, abuses antibiotic phenomenon general.Although made in recent years the medicine residual standard that much meets international standard, and constantly the use of standard feed additive is stipulated, but the consciousness at our detection method, monitoring system and foster rabbit family relatively lags behind, add poor management, become Carnis Leporis outlet and sold inside the country hidden danger, greatly reduce the market competitiveness of China's Carnis Leporis.

Summary of the invention

Technical problem to be solved by this invention is, to support on a small scale rabbit as main, abuses medicine for China's aquaculture, abuses antibiotic phenomenon general; And the consciousness that China has detection method, monitoring system and foster rabbit family now relatively lags behind, add poor management, become Carnis Leporis outlet and sold inside the country all undesirable, greatly reduce the shortcoming of the market competitiveness of China's Carnis Leporis, adopt brand-new Chinese medicine additive, reach the requirement of pollution-free food pure natural; Develop a kind of pure Chinese medicinal preparation for the treatment of rabbit diarrhoea, to solve above-mentioned technical barrier.

For solving the problems of the technologies described above, the invention provides a kind of Chinese medicine preparation for the treatment of rabbit diarrhoea, each crude drug comprises in the described Chinese medicine preparation: Herba Andrographis, Pericarpium Granati, Herba Patriniae, Radix Sanguisorbae, the Radix Pulsatillae, Radix Angelicae Sinensis, the Pseudobulbus Bletillae (Rhizoma Bletillae), Bulbus Allii Macrostemonis, Massa Medicata Fermentata (parched to brown), the Rhizoma Atractylodis Macrocephalae, the Radix Rehmanniae, Herba Hedyotidis Diffusae, Herba Taraxaci, Rhizoma Coptidis, Cortex Fraxini, Pericarpium Arecae, Radix Codonopsis, Radix Scutellariae, Rhizoma Smilacis Glabrae and Herba Artemisiae Annuae.

The Chinese medicine preparation of described treatment rabbit diarrhoea, the ratio of weight and number of each crude drug can be preferably in the described Chinese medicine preparation: Herba Andrographis 10～20 weight portions, Pericarpium Granati 10～20 weight portions, Herba Patriniae 10～20 weight portions, Radix Sanguisorbae 10～20 weight portions, the Radix Pulsatillae 10～20 weight portions, Radix Angelicae Sinensis 10～20 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 weight portions, Bulbus Allii Macrostemonis 10～20 weight portions, Massa Medicata Fermentata (parched to brown) 10～20 weight portions, the Rhizoma Atractylodis Macrocephalae 10～20 weight portions, the Radix Rehmanniae 10～20 weight portions, Herba Hedyotidis Diffusae 10～20 weight portions, Herba Taraxaci 10～20 weight portions, Rhizoma Coptidis 10～20 weight portions, Cortex Fraxini 10～20 weight portions, Pericarpium Arecae 10～20 weight portions, Radix Codonopsis 10～20 weight portions, Radix Scutellariae 10～20 weight portions, Rhizoma Smilacis Glabrae 10～20 weight portions and Herba Artemisiae Annuae 10～20 weight portions.

The Chinese medicine preparation of described treatment rabbit diarrhoea, the ratio of weight and number of each crude drug can also be preferably in its described Chinese medicine preparation: Herba Andrographis 10～15 weight portions, Pericarpium Granati 10～15 weight portions, Herba Patriniae 10～15 weight portions, Radix Sanguisorbae 10～15 weight portions, the Radix Pulsatillae 10～15 weight portions, Radix Angelicae Sinensis 10～15 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～15 weight portions, Bulbus Allii Macrostemonis 10～15 weight portions, Massa Medicata Fermentata (parched to brown) 10～15 weight portions, the Rhizoma Atractylodis Macrocephalae 10～15 weight portions, the Radix Rehmanniae 10～15 weight portions, Herba Hedyotidis Diffusae 10～20 weight portions, Herba Taraxaci 10～20 weight portions, Rhizoma Coptidis 10～20 weight portions, Cortex Fraxini 10～20 weight portions, Pericarpium Arecae 10～20 weight portions, Radix Codonopsis 10～20 weight portions, Radix Scutellariae 10～20 weight portions, Rhizoma Smilacis Glabrae 10～20 weight portions and Herba Artemisiae Annuae 10～20 weight portions.

The Chinese medicine preparation of described treatment rabbit diarrhoea, the ratio of weight and number of each crude drug also can be preferably in its described Chinese medicine preparation: Herba Andrographis 10～20 weight portions, Pericarpium Granati 10～20 weight portions, Herba Patriniae 10～20 weight portions, Radix Sanguisorbae 10～20 weight portions, the Radix Pulsatillae 10～20 weight portions, Radix Angelicae Sinensis 10～20 weight portions, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10～20 weight portions, Bulbus Allii Macrostemonis 10～20 weight portions, Massa Medicata Fermentata (parched to brown) 10～20 weight portions, the Rhizoma Atractylodis Macrocephalae 10～20 weight portions, the Radix Rehmanniae 10～20 weight portions, Herba Hedyotidis Diffusae 10～15 weight portions, Herba Taraxaci 10～15 weight portions, Rhizoma Coptidis 10～15 weight portions, Cortex Fraxini 10～15 weight portions, Pericarpium Arecae 10～15 weight portions, Radix Codonopsis 10～15 weight portions, Radix Scutellariae 10～15 weight portions, Rhizoma Smilacis Glabrae 10～15 weight portions and Herba Artemisiae Annuae 10～15 weight portions.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method for the treatment of the Chinese medicine preparation of rabbit diarrhoea, and the preparation process of its described Chinese medicine preparation is: the crude drug with described Chinese medicine additive shreds, refines into fine powder first; Mix again Mel and granulate, put into granulator and granulate.

The preparation method of the Chinese medicine preparation for the treatment of rabbit diarrhoea, when described Chinese medicine preparation granulation, granule was the cylinder of diameter 1cm～2cm when molding was granulated.

The preparation method of the Chinese medicine preparation of described treatment rabbit diarrhoea, its preparation process can be for: the first step first with described crude drug by described ratio of weight and number co-grinding, cross 100～120 mesh sieves, the rear fine powder that sieves is for subsequent use.

The preparation method of the Chinese medicine preparation of described treatment rabbit diarrhoea, its preparation process can for: second step adds unsifted coarse granule medicine in the ethanol of 10 times of weight portions, heating and refluxing extraction 3 times, each 1～2 hour, filtrate is merged, get medicinal liquid, with the medicinal liquid stand for standby use; Extract medicinal liquids with above-mentioned three times and merge, being concentrated into liquor strength is 0.6g crude drug/mL, behind the sucking filtration, when the relative density of filtrate is about 20 ℃ 1.08; Be decompressed to 0.03-0.05MPa, temperature remains on 70-80 ℃ extractum, extractum is put into oven for drying make powdery medicated powder.

The preparation method of the Chinese medicine preparation of described treatment rabbit diarrhoea, its preparation process can be mixed with the fine powder that sieves for the medicated powder that: the 3rd step obtains after will refining; The medicated powder that mixes adds proper honey and stirs into pasty state, puts into granulator and granulates.

The Chinese medicine preparation of described treatment rabbit diarrhoea, its described preparation formulation can be granule, powder, plaster or ointment; Can mix in the concentrated feed during use or Direct-fed.

The present invention is directed to and easily send out diarrhoea in the rabbit raising, add and go dampness, the Chinese medicine additive of strengthening the spleen and stomach, invigorating the spleen and replenishing QI, enhancing premunition; Direct bactericidal not only, and can regulate the immune function of body, have non-specific antibacterial action, and do not produce dependency; Can also promote the rabbit growth, abandon chemobiotic to the injury of rabbit body, brand-new Chinese medicine additive is adopted in the side effect of remote-effects food safety, reaches the requirement of pollution-free food pure natural.Selected Chinese herbal medicine not only contains abundant aminoacid, vitamin, trace element and unknown growth regulator, can directly promote the digestibility and utilization of nutrient substance, especially to the targeted curative effect of the diarrhoea of rabbit.Chinese herbal medicine additive can improve digestibility, the utilization rate of nutrient substance, strengthens the metabolism of poultry, and blood circulation promoting improves the speed of growth.

The specific embodiment

In the raising of rabbit, diarrhoea is the arch-criminal who causes the rabbit rate of animals delivered to the slaughter-house low always.And use in a large number antibiotic, and cause again the rabbit meat health not up to standard, drug residue exceeds standard, and quality does not reach importer's requirement, becomes vicious cycle.The scale of the existing fr raising rabbits of China is less, and it is general to abuse the phenomenon of medicine.Although made in recent years the use regulation of the also continuous standard feed additive of medicine residual standard that much meets international standard, but the consciousness at our detection method, monitoring system and foster rabbit family relatively lags behind, add poor management, become Carnis Leporis outlet and sold inside the country hidden danger.

Chinese herbal medicine not only self can be killed or the pathogenic microorganisms such as anti-bacteria or parasite, also non-specific antimicrobial all active factors of adjustable motivation body are comprehensive kills or suppresses pathogenic microorganism, search to the bottom, namely reach anti-microbial effect from enhancing body organ-tissue antibacterial ability and structure metabolism two aspects that act on antibacterial.As, Flos Lonicerae, Fructus Forsythiae, Herba Taraxaci etc. have broad-spectrum antibacterial action; The tool antivirus actions such as Rhizoma Coptidis, Radix Isatidis, Flos Lonicerae; The tool antifungic actions such as Radix Sophorae Flavescentis, Rhizoma Smilacis Glabrae, Cortex Dictamni; The anti-spirillum effects of tool such as Artemisia annua L., the Radix Rehmanniae, Cortex Phellodendri; Folium Isatidis has the effect of the pathogenetic bacterias such as anti-dysentery bacterium, meningococcus and leptospira; Fructus Capsici has anti-Staphylococcus aureus, escherichia coli, bacillus subtilis and aspergillus niger, penicillium sp effect.Xiao Sang alkali in Rhizoma Coptidis, the Cortex Phellodendri has significant inhibition to multiple gram positive bacteria and negative bacterium, is strong to the bacteriostasis of dysentery bacterium, staphylococcus aureus especially; Chinese medicine formula Cortex Fraxini, Rhizoma Atractylodis, Cortex Phellodendri, the Radix Pulsatillae etc. can effectively prevent and treat Pullorum Disease has obvious inhibitory or killing effect to Salmonella, according to another report, add 1% Chinese herbal compound preparation in the daily ration and have stronger anticoccidial effect, and long-term interpolation is better than the short-term additive effect.

Chinese herbal medicine itself is not hormone, but can play the regulating action similar to hormone to body, and can prevent. end, alleviate or eliminate the toxic and side effects of exogenous hormone.As, the tool estrogen-like effectss such as Rhizoma Cyperi, Radix Angelicae Sinensis, Radix Glycyrrhizae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Fructus Cnidii; The tool androgen-like actions such as excessive sheep mat, Radix Ginseng, Cordyceps: the tool epinephrine sample effects such as Herba Asari, Radix Aconiti Lateralis Preparata, Fructus Evodiae, Rhizoma Alpiniae Officinarum, Fructus Schisandrae Chinensis; The tool thyroliberin sample effects such as Cornu Bubali, Herba Andrographis, Radix Tripterygii Wilfordii; The carbamylmethylcholine sample effects such as Semen Ziziphi Spinosae, Fructus Lycii, Bulbus Allii.

The general abdominal part of sick rabbit expands, hypourocrinia and be canescence, and gastrointestinal peristalsis strengthens, and has the seasonal disease rabbit to present stomachache.Sick rabbit down in spirits, astasia, weakness, fervescence, disorderly thick by hair, poor appetite or absolutely useless, and diarrhoea appears, begin to be yellow loose stool, later stage is changed taupe or black watery stools into, have just in band blood or open-minded film is arranged, flavor is stench, most of sick rabbit hind legs, anus and afterbody are polluted by hair, and indivedual serious anuses stop up.Disease rabbit coolness of extremities, eye socket sink, and becomes thin rapidly, death in 2-3 days after the general morbidity, the sustainable 5-7 of some courses of disease days, this becomes raised the topmost loss in family.

Rabbit is plant-eating animal, and the Ying Yicao that feeds is main, and concentrate is auxiliary, suitably mend with coarse fodder, be strictly on guard against that concentrate is too much or crude fiber content is little, this point is easily out in the cold, though crude fibre is little easy to digest, but can fill gastrointestinal tract, stimulating gastrointestinal road mucosa is safeguarded intestinal smooth muscle system tonicity, reduce caecum, colon load is avoided food to form agglomerate in gastrointestinal and is caused that diarrhoea appears in enteritis, and crude fiber content should maintain in the 12-6% scope in the daily ration.

Choosing medical herbs is safe feed additive, follow Traditional Chinese Medicine theory and modern animal nutrition principle always, have with food homology, consubstantiality, with the characteristics of usefulness, have the dual function of nutrition and medicine concurrently, its immunity substance mass-energy enhancing human body immunity function, improve animal anti-stress, anti-disease ability, improve breeding performonce fo animals, go back simultaneously toxic side effect little, have no drug resistance, be difficult for the advantages such as residual.Facts have proved, Chinese herbal feed additive is applied to the energy Effective Raise rate of animals delivered to the slaughter-house in the rabbit raising, and survival rate is improved the Carnis Leporis mouthfeel, can directly affect ecological benefits, economic benefit and social benefit.

Specific embodiment 1: get Herba Andrographis 1000g, Pericarpium Granati 1000g, Herba Patriniae 1000g, Radix Sanguisorbae 1000g, Radix Pulsatillae 1000g, Radix Angelicae Sinensis 1000g, Pseudobulbus Bletillae (Rhizoma Bletillae) 1000g, Bulbus Allii Macrostemonis 1000g, Massa Medicata Fermentata (parched to brown) 1000g, Rhizoma Atractylodis Macrocephalae 1000g, Radix Rehmanniae 1000g, Herba Hedyotidis Diffusae 1000g, Herba Taraxaci 1000g, Rhizoma Coptidis 1000g, Cortex Fraxini 1000g, Pericarpium Arecae 1000g, Radix Codonopsis 1000g, Radix Scutellariae 1000g, Rhizoma Smilacis Glabrae 1000g and the agent of Herba Artemisiae Annuae 1000g granulation.

Specific embodiment 2: get Herba Andrographis 1000g, Pericarpium Granati 1000g, Herba Patriniae 1000g, Radix Sanguisorbae 1000g, Radix Pulsatillae 1000g, Radix Angelicae Sinensis 1000g, Pseudobulbus Bletillae (Rhizoma Bletillae) 1000g, Bulbus Allii Macrostemonis 1000g, Massa Medicata Fermentata (parched to brown) 1000g, Rhizoma Atractylodis Macrocephalae 1000g, Radix Rehmanniae 1000g, Herba Hedyotidis Diffusae 1000g, Herba Taraxaci 1000g, Rhizoma Coptidis 1000g, Cortex Fraxini 1000g, Pericarpium Arecae 1000g, Radix Codonopsis 1000g, Radix Scutellariae 1000g, Rhizoma Smilacis Glabrae 1000g and Herba Artemisiae Annuae 1000g, first described crude drug is pressed described ratio of weight and number co-grinding, cross 100～120 mesh sieves, the rear fine powder that sieves is for subsequent use, again the described ratio of weight and number of unsifted coarse granule is added in the ethanol of 10 times of weight portions, heating and refluxing extraction 3 times, each 1～2 hour, filtrate is merged, get medicinal liquid, with the medicinal liquid stand for standby use; Extract medicinal liquids with above-mentioned three times and merge, being concentrated into liquor strength is 0.8g crude drug/mL, behind the sucking filtration, when the relative density of filtrate is about 20 ℃ 1.06; Be decompressed to 0.03-0.06MPa, temperature remains on 70-80 ℃ extractum, extractum is put into oven for drying make powdery medicated powder; Mix twice medicated powder and mix Mel, put into granulator and make 1-2 centimetre granule.

Specific embodiment 3: get Herba Andrographis 1000g, Pericarpium Granati 1000g, Herba Patriniae 1000g, Radix Sanguisorbae 1000g, Radix Pulsatillae 1000g, Radix Angelicae Sinensis 1000g, Pseudobulbus Bletillae (Rhizoma Bletillae) 1000g, Bulbus Allii Macrostemonis 1000g, Massa Medicata Fermentata (parched to brown) 1000g, Rhizoma Atractylodis Macrocephalae 1000g, Radix Rehmanniae 1000g, Herba Hedyotidis Diffusae 1000g, Herba Taraxaci 1000g, Rhizoma Coptidis 1000g, Cortex Fraxini 1000g, Pericarpium Arecae 1000g, Radix Codonopsis 1000g, Radix Scutellariae 1000g, Rhizoma Smilacis Glabrae 1000g and Herba Artemisiae Annuae 1000g shredded 100～120 mesh sieves with the above-mentioned raw materials medical material, the rear fine powder that sieves is for subsequent use, coarse granule is put into the acid and alkali-resistance infusion kettle, at room temperature, steeped 20 days with the above ethanol of 70 degree, the coarse granule medicinal residues that dipping is good squeeze to pulverize and filter, and get filtrate after the separation; Described filtrate heating is concentrated into pasty state, puts into drying in oven, grind to form fine powder after the cooling; Add the above-mentioned fine powder that sieves, be mixed into powder, mix twice medicated powder and become powder.

Specific embodiment 4: first with crude drug Herba Andrographis 1000g, Pericarpium Granati 1000g, Herba Patriniae 1000g, Radix Sanguisorbae 1000g, Radix Pulsatillae 1000g, Radix Angelicae Sinensis 1000g, Pseudobulbus Bletillae (Rhizoma Bletillae) 1000g, Bulbus Allii Macrostemonis 1000g, Massa Medicata Fermentata (parched to brown) 1000g, Rhizoma Atractylodis Macrocephalae 1000g, Radix Rehmanniae 1000g, Herba Hedyotidis Diffusae 1000g, Herba Taraxaci 1000g, Rhizoma Coptidis 1000g, Cortex Fraxini 1000g, Pericarpium Arecae 1000g, Radix Codonopsis 1000g, Radix Scutellariae 1000g, Rhizoma Smilacis Glabrae 1000g and Herba Artemisiae Annuae 1000g, shredded 100～120 mesh sieves, the rear fine powder that sieves is for subsequent use, coarse granule is put into the acid and alkali-resistance infusion kettle, at room temperature, steeped 15～30 days with the above ethanol of 90 degree, liquid and medicinal residues that dipping is good carry out squeeze and filter, get filtrate after the separation; The heating of described filtrate is concentrated, when the relative density of filtrate is about 20 ℃ 1.06; Be decompressed to 0.03-0.06MPa, temperature remains on 70-80 ℃ extractum, makes extractum.

The various properties of Chinese materia medica in the Chinese medicine additive:

Its sweet in the mouth of Radix Angelicae Sinensis and weighing, therefore specially can enrich blood, its gas is light and hot, thus again can promoting the circulation of blood, invigorating middle warmer has, in the row benefit is arranged, really the gas medicine in the blood, the also panacea in the blood.About mending to mend then of assistant, thus can support nutrition blood, the QI invigorating spermatogenesis, settling five organs, body by force, beneficial mind, all tangible deficient diseases, nothing is unsuitable.Assistant logical to attack then, therefore the pain relieving constipation of dispelling, relieving rigidity of muscles and joints is controlled contracture, paralysis, the card such as dry, puckery.Nutrient QI being deficient and exterior syndrome being not relieved person, assistant is with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, the Biao Weire that can fall apart greatly, and show not hold back the person, and help with Radix Et Rhizoma Rhei and so on, again can consolidating superficial resistance.Precisely because gas Xin Erdong, therefore want its quiet person when keeping away it, the sliding benefaction of property is defecated and is not consolidated the person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.

The Radix Astragali is take tonify deficiency as main, can invigorating QI to consolidate the body surface resistance, and diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.Be used for that insufficiency of vital energy and blood, skin infection cave in, difficulty in discharging of pus or burst for a long time and do not hold back the person.The Radix Astragali has the function of good promoting pus discharge and tissue regeneration strengthening, i.e. the abscess suppuration granulation promoting of for a long time disunion.Modern medicine study shows, the Radix Astragali includes and multiple antibiotic effective ingredient, and immunologic function that can enhancing body, therefore can also be used for the generation of some infectious disease of prevention." book on Chinese herbal medicine meets former " carries: " Radix Astragali can all void of tonifying five ZANG-organs, controls the stringy pulse spontaneous perspiration, rushes down YIN-fire, removes lung-heat, losslessly then sends out, and has antiperspirant then to end." be the good medicine of promoting resistance and defence disease.

Radix Scutellariae has heat clearing and damp drying, and removing heat from blood is antiabortive, detoxicating functions.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.The clinical practice of Radix Scutellariae is antibiotic not worse than Rhizoma Coptidis, and does not develop immunity to drugs.We are used in the fungal culture miscellaneous bacteria and infect special severity by the strong characteristics of broad-spectrum antibacterial action, and are fine with Radix Scutellariae extracting solution effect." Bencao Jingshu ": Radix Scutellariae, its property is respectful clearly, so evil removing: so bitter in the mouth dampness; So YIN-cold wins heat, therefore main all heat.All hot persons, pathogenic heat with all over heat also, jaundice, intestinal be out-of-the-way, let out dysentery, the disease of all damp and hot victory is also analysed it, then all diseases are from recover.Bitter cold can be except damp and hot, thus small intestinal profit and water from pursuing, the source is clearly then flowed also clean.The blood person of closing, excess-heat is at blood system, and amenorrhea is made us obstructed in instant heating human blood chamber, damp and hot solution, then Ying Qi is clearly and voluntarily also.Malignant boil cellulitis erosion person, heat in blood is then stayed knot, and is that carbuncle stain is mashed also: the acute scleritis person, the fire of moxibustion blood trouble also, the removing heat from blood heat extraction, then spontaneous recovery also.

The Rhizoma Atractylodis Macrocephalae can strengthen shrinkage amplitude, the contraction frequency of intestinal smooth muscle significantly, prolongs intestinal smooth muscle contraction time under anaerobic conditions, adjusts the gastrointestinal motility function, has antiulcer, protects the liver the effect with the enhancing human body immunity function.Rhizoma Atractylodis Macrocephalae bitter in the mouth, sweet, temperature.Return spleen, stomach warp.Cure mainly invigorating the spleen and benefiting QI, the dampness diuretic, hidroschesis, antiabortive.Be used for insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, phlegm retention vertigo and palpitation, edema, spontaneous perspiration, frequent fetal movement.The Rhizoma Atractylodis Macrocephalae has cytotoxicity to oncocyte, can reduce the rate of increase of oncocyte, lowers the aggressive of tumor tissue, improves the ability of body antitumor reaction." property of medicine opinion " carries it: monarch, and sweet in the mouth, suffering, nontoxic.Can main large migratory arthralgia, diarrhea due to disorder of qi for many years, trusted subordinate's distending pain, the broken dyspepsia that disappears, sputum is removed in appetizing, except cold and heat, lets out under ending.Interarea light is pleased, and preserves youthful looks, and goes to deceive.The swelling of harnessing the river is full, tells vomiting, and cold type of pain in the abdomen is vomited and diarrhoea incessantly, and the insufficiency of stomach-QI cold-dysentery.

Contain the multiple health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, inulin in the Herba Taraxaci plant, the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting are arranged.Herba Taraxaci contains protein, fat, carbohydrate, trace element and vitamin etc. simultaneously, and abundant nutritive value is arranged, and is raw-eaten, stir-fry and eat, cook soup, is the plant of dietotherapeutic.The Dandelion Compositae belongs to herbaceos perennial.It is the plant of dietotherapeutic.According to the Compendium of Material Medica record, the flat sweet and slightly bitter taste of its property has heat-clearing and toxic substances removing, dispersing swelling and dissipating binds and galgctogogue action, and is very effective to the treatment mastitis.No matter fry in shallow oil juice oral, or the smash to mud external application, effect is all arranged.In addition, Herba Taraxaci also has the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting, is widely used in clinical.Compendium of Material Medica has a cloud: " the tender Seedling of Herba Taraxaci is edible, eats that to control infectious disease especially good raw." the successive dynasties Medical monographs such as Shennong's Herbal, Tang Materia Medica, " Chinese medicine voluminous dictionary " all give high evaluation.

Herba Artemisiae Annuae is feverfew Herba Artemisiae annuae Artemisia annua L. dry aerial parts.The Herba Artemisiae Annuae clearing away summer-heat is except steaming preventing the attack (or recurrence) of malaria.Be used for the pathogenic summer-heat heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to YIN-deficiency consumptive fever, malaria cold and heat, jaundice due to damp-heat.It is a kind of antimalarial drug of cheapness.Herba Artemisiae Annuae decocting liquid has stronger bacteriostasis to staphylococcus epidermidis, micrococcus catarrhalis, anthrax bacillus, diphtheria corynebacterium, and staphylococcus aureus, bacillus pyocyaneus, dysentery bacterium, tuberculosis stalk bacterium etc. are also had certain inhibitory action.Herba Artemisiae Annuae volatile oil has bacteriostasis to all dermatophytosises when 0.25% concentration, when 1% concentration, all dermatophytosises are had bactericidal action.Herba Artemisiae Annuae have the effect of resisiting influenza virus.Sodium artesunate is to golden Fructus Vitis viniferae, shigella flexneri, escherichia coli, micrococcus catarrhalis, and first type and paratyphoid B stalk bacterium all have certain antibacterial action.Sitosterol in the Herba Artemisiae Annuae and stigmasterol also have antivirus action.

Pseudobulbus Bletillae (Rhizoma Bletillae) tonifying the lung, hemostasis, detumescence, granulation promoting, sore.Control lung and hinder hemoptysis, epistaxis, incised wound is hemorrhage, swollen ulcer drug, ulcer pain, the soup fire is burnt, rhagadia manus et pedis." herbal classic ": " main carbuncle malignant boil loses cellulitis, the dead flesh of impairment of YIN, pathogen in the stomach, the harmful wind miliaria is slow not to be received." antibiotic, antifungic action: the immersion of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol has inhibitory action to staphylococcus aureus, bacillus subtilis, Bacillus tuberculosis; The water logging agent has inhibitory action to microsporon audouini; Contained 3 biphenyl classes and 2 the luxuriant and rich with fragrance compounds of two hydrogen of Pseudobulbus Bletillae (Rhizoma Bletillae) have inhibitory action to staphylococcus aureus, bacillus subtilis, Candida albicans ATCC1057 and Trichophyton QM248.

Cortex Fraxini English name: CORTEX FRAXINI.Latin plant animal mineral name: Fraxinus rhynchophylla Hance F.Chinensis Roxb.var.rhynchopylla (Hance) Hemsl.] and the nature and flavor hardship, cold.Heat clearing and damp drying Chinese medicine.Cure mainly: heat clearing and damp drying, liver heat removing and eyesight improving, antiasthmatic-antitussive.Be used for pyretic toxicity dysentery, conjunctival congestion and swelling pain, the living cataracta of order.Antibacterial action: Cortex Fraxini decoct pastille flat band method, 1:50 is to staphylococcus aureus, dysentery bacterium, 1:20 has inhibitory action to escherichia coli etc. and in vitro tests shows, Cortex Fraxini is inhibited to various bacteria such as staphylococcus epidermidis, micrococcus catarrhalis, bacillus pyocyaneus. and anti-inflammatory and analgesic effect: eaculin has inhibitory action to experimental arthritis, and faint analgesic activity is arranged.

Its Chinese patent medicine bitter in the mouth, light of Herba Hedyotidis Diffusae, cold in nature.Main effect is heat-clearing and toxic substances removing, medicinal powder for relieving pain knot, promoting urination to remove dampness.Outstanding kind treatment all kinds inflammation.In clinical practice, if find that the Herba Hedyotidis Diffusae compatibility is proper, can treat various diseases.

The Rhizoma Smilacis Glabrae detoxicating and resolving stagnation of pathogens, dispelling wind and removing obstruction in the collateral, dampness removing is let out turbid.Cure mainly syphilis, sore throat, carbuncle malignant boil, scrofula, carcinoma, muscles and bones spasm and pain, edema, stranguria with turbid discharge, the beriberi of having loose bowels, eczema scabies, mercurialism." detailed outline ": strengthening the spleen and stomach, bone and muscle strengthening, curing rheumatism, sharp joint, stopping leak rushes down.Control the contracture osteodynia; The malignant boil carbuncle.Separate hydrargyrum powder, Vermilion poison.

The Herba Patriniae heat-clearing and toxic substances removing, removing heat from blood, eliminating carbuncle evacuation of pus, stasis-dispelling and pain-killing.Be used for acute appendicitis, the high heat of lung abscess is coughed vomiting bloody pus, pyretic toxicity skin ulcer furuncle, and furuncle is bitterly swollen, breast abdomen pain, appendicitis, enteritis, dysentery, puerperal abdonimal pain, dysmenorrhea.Also can make the domestic animal feedstuff.Herba Ixeritis chinensis belongs to medium herbage.The tender succulence of stem and leaf, sheep, goat happiness food when blue or green aquatic foods.According to herdsman's reflection, it has the effect of fattening spring to little poultry.Cattle, horse also search for food on a small quantity, but can not utilize after withered.In addition, it still raises pigs, supports the good feedstuff of rabbit, foster fowl.Its nutritive value is also higher, according to one's analysis, contains the crude fibre of higher thick protein and low amount at flowering fruit bearing stage.In various amino acid whose content, higher with the content of lysine, threonine, valine.Contain 7018 milligrams of vitamin Cs in the 100 gram herbs.

Pericarpium Arecae formal name used at school: Pericarpium Arecae (drawing); Dry peel for babassu Semen Arecae Areca catechu L..Acrid in the mouth; Slightly warm in nature.Return spleen; Stomach; Large intestine; Small intestine meridian.Modern study, these product have excited gastrointestinal smooth muscle, short digestive tract power effect, and the effect such as the fibrinolysis of promotion is arranged." Kaibao Bencao ": the main cold and hot gas abdomen of making a psychological attack, large intestine is stopped up poison, expectorant diaphragm, the belching of acid from stomach.And fry in shallow oil with Jiang Yantong, enter to dredge the gas medicine good." detailed outline ": lowering the adverse flow of QI, aqueous vapor edema in the skin that disappears, beriberi is stopped up contrary, subtertian malaria feeling of fullness, nausea malignant histocytosis feeling of distension and oppression." book on Chinese herbal medicine is new again ": eliminating the pathogens from the lung, easing stomach-QI, dampness removing chases after wind, wide intestinal detumescence, reason waist beriberi, treating malaria disease dysentery.

The Rhizoma Coptidis heat clearing and damp drying, eliminating fire and detoxication.Be used for damp and hot feeling of fullness, the vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion, the carbuncle furuncle is quenched one's thirst in toothache; External treatment eczema, eczema, auditory meatus is suppurated.The kind clear part of the body cavity above the diaphragm housing the heart and lungs of Rhizoma Coptidis (processed with wine) is burning hot.Be used for conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.Be used for cold and heat and tie mutually, damp and hot middle resistance, feeling of fullness vomiting.Cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.Be used for incoordination between the liver and stomach, the vomiting acid regurgitation.Be used for the diseases such as retention of damp-heat in the interior, the intestines and stomach are damp and hot, vomiting, dysentery.Join Radix Scutellariae, Radix Et Rhizoma Rhei etc., can control the card of retention of damp-heat in the interior.To the damp and hot the intestines and stomach of being reluctant to leave, often cooperate the Rhizoma Pinelliae, Caulis Bambusae In Taenia; Join the Radix Aucklandiae, Radix Scutellariae, Radix Puerariae etc. to control dysentery.

Herba Andrographis has heat-clearing and toxic substances removing, antiinflammatory, reducing swelling and alleviating pain effect.Cure mainly bacillary dysentery, urinary tract infection, acute tonsillitis, enteritis, pharyngolaryngitis, pneumonia and influenza etc., external can be treated furuncle toxic swelling, trauma infection contamination etc.Heat-clearing and toxic substances removing, the removing heat from blood detumescence.Control acute bacillary dysentery, gastroenteritis, flu, epidemic encephalitis, tracheitis, pneumonia, pertussis, pulmonary tuberculosis, lung abscess, cholecystitis, hypertension, epistaxis, oropharynx swells and ache, furuncle carbuncle, burn due to hot liquid or fire, venom." Guangxi Chinese herbal medicine ": the hemostasis removing heat from blood, removing toxic substances and promoting tissue regeneration is controlled lung abscess, stomatitis.

The effect that Radix Rehmanniae has clearing away heat and cooling blood, tonifying YIN to promote the production of body fluid.Li Shizhen (1518-1593 A.D.) to the evaluation of Radix Rehmanniae is: " one hundred days of clothes are rosy cheeks, make light of one's life by commiting suicide not old in 3 years." Radix Rehmanniae heat clearing away, promote the production of body fluid, moisturize, laxation, removing blood stasis with potent drugs, tissue regeneration promoting, pain relieving, regulating menstruation, the incised wound stasis of blood, removing heat from blood, hemostasis; Bright Radix Rehmanniae sweet in the mouth bitterness Great Cold acts on similarly to Radix Rehmanniae, and the power of YIN nourishing is slightly inferior, but clearing away heat and promoting production of body fluid, the power of cooling blood for hemostasis is stronger.

Massa Medicata Fermentata mixes the processed goods that forms by fermentation for whole wheat flour and other drug (Herba Artemisiae Annuae, Herba Xanthii, the buds such as Herba polygoni hydropiperis, Semen Armeniacae Amarum, Semen Phaseoli).Get Massa Medicata Fermentata and put to fry in the pot to appearance and be coke black, inner coke yellow, take out, spray the water that disappears, cool, be Massa Medicata Fermentata (parched to brown).Massa Medicata Fermentata forms through fermentation, and the product of all fermentations have the digestant effect of strengthening the spleen and stomach, therefore, for bad, the feeling of stuffiness in chest abdominal distention of the digestion due to the diet internal injury effect is arranged quite.

Radix Sanguisorbae property of medicine picric acid; Cold; Nontoxic, return liver; Lung; Kidney; Large intestine channel, effect cooling blood for hemostasis, heat-clearing and toxic substances removing, detumescence sore.Effect classification hemorrhage; Blood-cooling hemostatic.Cure mainly haematemesis, spitting of blood, epistaxis, hematuria is had blood in stool, hemorrhoidal bleeding, dysentery, metrorrhagia, leucorrhea with red and white discharge, sore, carbuncle and painful swelling, eczema, pudendal pruritus, burn due to hot liquid or fire, snake bite and insect sting." detailed outline ": Radix Sanguisorbae, except part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels heat, control the defecation blood disorder.Hemostasis is got and is cut off sheet and fry usefulness, and its tip then can promoting the circulation of blood, can not know.The Yang Shiying cloud: all skin ulcer pain persons add Radix Sanguisorbae, and the person of itching adds Radix Scutellariae.

Pericarpium Granati has relieving diarrhea with astringents, hemostasis, the effect of anthelmintic." property of medicine opinion " carries it: " control muscles and bones wind, the waist foot is unsuccessful, the anxious pain of walking contraction.Main astringing intestine to stop diarrhea ends red white dysentery.Extracting juice ends under the order tear, controls Lou essence." supplement to the Herbal carries it: " main ascarid.Decoction." " the southern regions of the Yunnan Province book on Chinese herbal medicine " carries it: " controls with the passing of time watery diarrhea, simmers clothes with frying Saccharum Sinensis Roxb., controls again the dysentery pus and blood, the large intestine hematochezia." " fried in shallow oil with Fructus Aristolochiae and controlled children's's infantile malnutrition worm." experiment in vitro shows: Pericarpium Granati is to escherichia coli; bacterium sonnei; Bacillus proteus; Bacillus typhi; Salmonella paratyphi; bacillus pyocyaneus, 7 kinds of Grain-negative enteral such as vibrio cholera pathogenic bacterium all have inhibitory action. in vitro, the Pericarpium Granati decoct all has antibacterial action to 4 kinds of dysentery bacteriums, but difference to some extent on the degree, with to shigella effect the strongest (1:320), to Amur, bacterium flexneri takes second place (1:80), and to bacterium sonnei effect relatively poor (1:20). the antibacterial effect of flat band method is lower than test tube method. and its antibacterial action mechanism is relevant with its contained volume tannic acid.

Radix Pulsatillae bitter in the mouth, cold in nature; Return stomach, large intestine channel; Has heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, the effect of removing dampness and killing parasites; Cure mainly pyretic toxicity dysentery, epistaxis, internal hemorrhoid with bleeding, leukorrhagia, pudendal pruritus, the carbuncle skin ulcer, the scrofula Radix Pulsatillae is the key medicine of controlling amebic dysentery, alone larger dose namely produces effect.Commonly use into square Radix Pulsatillae Decoction, namely take this product as principal agent, cooperate Rhizoma Coptidis, Cortex Phellodendri, Cortex Fraxini to form, both the available amebic dysentery of controlling, the also available bacillary dysentery of controlling." book on Chinese herbal medicine justice " carries it: Radix Pulsatillae mildly bitter flavor and light, and the clear light weight of gas, " herbal classic " though the bitter temperature of meaning is so mad easily to cure mainly pyrexial malaria, and Zhong Jing and specially to control diarrhea of heat type with tenesmus, then the Bi Feiwen medicine as can be known.Its main pyretic toxicity dysentery admittedly can let out though say hardship, and elevate a turnable ladder taste clearing heat in QI system makes and does not sag, and then tenesmus all removes, really the tangible true essence of this medicine.

Bulbus Allii Macrostemonis is hot, bitter, temperature.Return lung, stomach, large intestine channel.Regulate the flow of vital energy, the chest stuffiness relieving activates yang, eliminating stagnation.Control the thorough back of the body of obstruction of qi in the chest and cardialgia, the gastral cavity painful abdominal mass is not relaxed, and retches, and is heavy behind the dysentery, furuncle.Activating YANG and eliminating stagnation, the circulation of qi promoting intestinal stasis relieving.Be used for thoracic obstruction pain, the phlegm retention cough with asthma is let out after the dysentery heavy.Supplement to the Herbal carries it: in the accent, main chronic diarrhea is not recovered, normal evil person in the large abdomen, but cook it more.

Chinese medicine additive pharmacology toxicity test

The pharmacology pharmacodynamic experiment:

1, acute toxicity test

The application rabbit carries out acute toxicity testing and shows: rabbit oral administration gavage Chinese medicine preparation granule of the present invention, under 488.8g crude drug/kg dosage, the light activity minimizing appears in rabbit after the administration, recover normal about 1 hour, Continuous Observation is 7 days after the administration, none animal dead, its overall health of patients, diet, to take the photograph water, urine and body weight gain all normal, and experimental result shows: the maximum dosage-feeding of rabbit oral administration gavage Chinese medicine preparation granule of the present invention is 488.8g crude drug/kg/d (LD50＞488.8g crude drug/kg).Chinese medicine preparation clinical application every day total amount of the present invention is 0.1g crude drug/kg/d; Point out this medicine acute toxicity low, clinical drug safety.

2, long term toxicity test

Select rabbit, give variable concentrations (18.0,6.0, the Chinese medicine preparation powder of the present invention of 2.0g crude drug/kg), every day gavage once, continuous 90 days, 24 hours each groups are lived and are killed 1/2 animal (male and female half and half) after the last administration, live extremely after all the other 1/2 animals continuation observed for 2 weeks.Duration of test is observed outward appearance, general behavior, food ration, the body weight change of animal, after the administration 90 days and carry out hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) in 2 weeks of drug withdrawal and the index checkings such as blood biochemical (AST, ALT, ALP, Glu, BUN, Crea, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology.Result of the test shows: preparation of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight gain all change without unusual; Three dosage groups and matched group hematological examination, blood biochemical are learned, the urine biochemical analysis is all in normal range, between group without significant difference; Each is organized the main organs histopathological examination and shows no obvious abnormalities.The These parameters drug withdrawal also has no change after 2 weeks.According to result of the test Chinese medicine preparation of the present invention high, medium and low three dosage (18.0,6.0, the 2.0g crude drug/kg) administration in continuous 90 days has no significant effect rabbit, without clear and definite toxicity target organ and sensitive indicator, the convalescent period observation also has no the retardance toxic reaction, and the dosage safety of pointing out Chinese medicine preparation of the present invention to use is higher.

3, animal local excitation test:

Skin anaphylactic test of the present invention and rabbit rectal application irritation test.The result shows: skin anaphylactic test, high dose group 0.8g (medicated powder)/kg, low dose group 0.1g (medicated powder)/kg, be equivalent to respectively to intend 20 times, 2.5 times of clinical people's consumption [0.04g (medicated powder)/kg], through the skin administration, be showed no animal and produce allergy; Rectal application irritation test high dose group 5.0g (medicated powder)/kg, low dose group 3.2g (medicated powder)/kg, be equivalent to respectively to intend 125,80 times of clinical people's consumption, observe mucous membrane of rectum all without phenomenons such as hyperemia, edema in setting time, histopathologic examination and blank group be no significant difference relatively, have no and obvious irritative response occurs, every group of its overall health of patients of Some Animals of retaining, body weight, breathing, circulation, central nervous system and extremity activity etc., all Non Apparent Abnormality reactions.

Pharmacological experiment

The curative effect of Chinese medicine preparation granule of the present invention is proved by following pharmacodynamics test and clinical prerun:

One. on the have loose bowels impact of model of rabbit due to the Oleum Ricini

Get 40 of rabbit, male and female half and half are divided into model control group, antibiotic (cereobiogen sheet, galactenzyme raw cook or yeast tablet group), granule high dose group of the present invention, low dose group at random.Gavage gives relative medicine 0.2ml/10gB.W. by organizing respectively, and model group gives the equal-volume normal saline, once a day, and continuous three days.After the last administration 40 minutes, every animal gavage gives the 0.2ml Oleum Ricini.Then be placed with the cell isolation cage of filter paper under rabbit being put into, one of every lattice.Observation index: muck counting; The filter paper that spreads one 10cm * 10cm area under every cell is made the muck counting.React the degree of having loose bowels with muck grain number, per hour changed filter paper once in front 1～6 hour, changed paper once in 7～24 hours, record the accumulation muck sum of each animal in 24 hours, compare with model control group, organize a t check with SPSS software.The results are shown in Table one.

The anti-diarrhea effect of showing to have loose bowels due to a pair of Oleum Ricini (X ± SD)

Annotate: compare * * P＜0.01, * * * P＜0.001 with model control group

From table one as seen, medicine of the present invention can significantly suppress due to the Oleum Ricini rabbit and has loose bowels.

Two. to the intestinal propulsion exercise test

Get 40 of rabbit, male and female half and half are divided into Normal group, antibiotic group (cereobiogen sheet, galactenzyme raw cook or yeast tablet group), high dose group of the present invention, low dose group at random.Gavage gives respectively relative medicine 0.2ml/10gB.W. by organizing respectively, and Normal group gives the equal-volume normal saline, once a day, and continuous four days.Each organizes rabbit beginning fasting 24 hours in the 3rd day after administration.Tested the 4th day, after the administration 1 hour, gavage 5% Insta-Char 0.2ml/10gB.W..After 20 minutes, take off cervical vertebra and put to death animal, open the abdominal cavity, separate mesentery, on the clip to pylorus, down to the intestinal tube between the ileocecus, place on the flat pallet, gently small intestinal is pulled into straight line, measure Length of intestine as " small intestinal total length ", the distance from pylorus to the prepared Chinese ink forward position is as " prepared Chinese ink is at the enteral advance distance ".Calculate the prepared Chinese ink ink propulsive rate, organize a t check with SPSS software.The results are shown in Table two.

The impact that table two moves on intestinal propulsion (X ± SD)

Annotate: compare * * P＜0.01, * * * P＜0.001 with Normal group.

By table two as seen, the present invention and the intestinal propulsion function all had obvious inhibitory action.

Illustrate:

Diarrhoea rabbit general abdominal part expands, hypourocrinia and be canescence, and gastrointestinal peristalsis strengthens, and has seasonal disease north to the Great Wall rabbit to present stomachache.Sick north to the Great Wall rabbit down in spirits, astasia, weakness, fervescence, disorderly thick by hair, poor appetite or absolutely useless, and diarrhoea appears, begin to be yellow loose stool, later stage is changed taupe or black watery stools into, have just in band blood or open-minded film is arranged, flavor is stench, most of sick north to the Great Wall rabbit hind leg, anus and afterbody are polluted by hair, and indivedual serious anuses stop up.Disease rabbit coolness of extremities, eye socket sink, and becomes thin rapidly, death in 2-3 days after the general morbidity, the sustainable 5-7 of some courses of disease days.

The personnel that feed often observe, and find that the disease rabbit in time moves to drying, warm isolation rabbit home, ascertain the reason, and take corresponding nursing intervention, prevent from infecting.In daily ration, add preventive medicine.

Rabbit home and place disappear the raising apparatus with hundred poisoning solution every other day will be with 3% lysol solution thorough disinfection once, and feces, the duty in time eliminated in cage, the house are changed the pad grass, maintenance rabbit home cleaning, dry and good ventilation.

Claims

1. a Chinese medicine preparation for the treatment of rabbit diarrhea, characterized in that, each bulk drug in the described Chinese medicine preparation comprises: Andrographis paniculata, pomegranate peel, Patrinia, Burnet, Pulsatilla, Angelica, Bletilla striata, Scallops, Jiao Shenqu, Atractylodes macrocephala, Habitat, Hedyotis diffusa, Dandelion, Coptis chinensis, Qinpi, Dabupi, Codonopsis pilosula, Scutellaria baicalensis, Smilax smilax and Artemisia annua.

2. The Chinese medicine preparation for treating rabbit diarrhea according to claim 1, characterized in that, the weight-to-number ratio of each crude drug in the Chinese medicine preparation is: 10-20 parts by weight of Andrographis paniculata, 10-20 parts by weight of pomegranate peel, 10-20 parts by weight of pomegranate peel, 10-20 parts by weight of Salmonella, 10-20 parts by weight of Burnet, 10-20 parts by weight of Pulsatilla sinensis, 10-20 parts by weight of Angelica sinensis, 10-20 parts by weight of Bletilla striata, 10-20 parts by weight of Scallops, 10-20 parts by weight of Jiao Shenqu 10-20 parts by weight of Atractylodes macrocephala, 10-20 parts by weight of raw land, 10-20 parts by weight of Hedyotis diffusa, 10-20 parts by weight of dandelion, 10-20 parts by weight of coptis, 10-20 parts by weight of bark, 10-20 parts by weight, 10-20 parts by weight of Codonopsis pilosula, 10-20 parts by weight of Scutellaria baicalensis, 10-20 parts by weight of Smilax smilax and 10-20 parts by weight of Artemisia annua.

3. The Chinese medicine preparation for treating rabbit diarrhea according to claim 1, characterized in that, the ratio of parts by weight of each crude drug in the Chinese medicine preparation is: 10-15 parts by weight of Andrographis paniculata, 10-15 parts by weight of pomegranate peel, 10-15 parts by weight of pomegranate peel, 10-15 parts by weight of Salmonella, 10-15 parts by weight of Burnet, 10-15 parts by weight of Pulsatilla sinensis, 10-15 parts by weight of Angelica sinensis, 10-15 parts by weight of Bletilla striata, 10-15 parts by weight of Scallops, 10-15 parts by weight of Jiao Shenqu 10-15 parts by weight of Atractylodes macrocephala, 10-15 parts by weight of raw land, 10-20 parts by weight of Hedyotis diffusa, 10-20 parts by weight of dandelion, 10-20 parts by weight of coptis, 10-20 parts by weight of bark, 10-20 parts by weight, 10-20 parts by weight of Codonopsis pilosula, 10-20 parts by weight of Scutellaria baicalensis, 10-20 parts by weight of Smilax smilax and 10-20 parts by weight of Artemisia annua.

4. The Chinese medicine preparation for treating rabbit diarrhea according to claim 1, characterized in that, the ratio of parts by weight of each crude drug in the Chinese medicine preparation is: 10-20 parts by weight of Andrographis paniculata, 10-20 parts by weight of pomegranate peel, 10-20 parts by weight of pomegranate peel, 10-20 parts by weight of Salmonella, 10-20 parts by weight of Burnet, 10-20 parts by weight of Pulsatilla sinensis, 10-20 parts by weight of Angelica sinensis, 10-20 parts by weight of Bletilla striata, 10-20 parts by weight of Scallops, 10-20 parts by weight of Jiao Shenqu 10-20 parts by weight of Atractylodes macrocephala, 10-20 parts by weight of raw land, 10-15 parts by weight of Hedyotis diffusa, 10-15 parts by weight of dandelion, 10-15 parts by weight of coptis, 10-15 parts by weight of bark, 10-15 parts by weight, 10-15 parts by weight of Codonopsis pilosula, 10-15 parts by weight of Scutellaria baicalensis, 10-15 parts by weight of Smilax smilax and 10-15 parts by weight of Artemisia annua.

5. A Chinese medicine preparation for the treatment of rabbit diarrhea as described in any one of claims 1-4, characterized in that, the preparation step of said Chinese medicine preparation is: earlier the crude drug of said Chinese medicine preparation is chopped, refined into a fine powder; then mixed with honey to granulate, and put into a granulator to granulate.

6. According to the preparation method of the traditional Chinese medicine preparation for treating rabbit diarrhea described in claim 5, it is characterized in that, when said Chinese medicine preparation is made into granules, the granules are cylinders with a diameter of 1 cm to 2 cm during granulation.

7. according to the preparation method of the traditional Chinese medicine preparation for the treatment of rabbit diarrhea described in claim 5, it is characterized in that, its preparation step is: the first step first described crude drug is mixed and pulverized by described weight and number ratio, crosses 100 ~ 120 mesh sieve, fine powder after sieving.

8. according to the preparation method of the Chinese medicine preparation for the treatment of rabbit diarrhea described in claim 5, it is characterized in that, its preparation step is: the second step adds the unsifted coarse grain medicine in the ethanol of 10 times by weight, heats to reflux Extract 3 times, each time for 1-2 hours, combine the filtrates to obtain the medicinal solution, and put the medicinal solution aside for later use; combine the above three extracted medicinal solutions, concentrate until the concentration of the medicinal solution is 0.6g crude drug/mL, and after suction filtration, The relative density of the filtrate is about 1.08 at 20°C; the extract is decompressed to 0.03-0.05MPa and the temperature is kept at 70-80°C, and the extract is dried in an oven to make powdered medicinal powder.

9. according to the preparation method of the Chinese medicine preparation of the treatment rabbit diarrhea described in claim 5, it is characterized in that, its preparation step is: the 3rd step mixes the medicated powder obtained after refining with the fine powder of sieving; Mixed medicated powder is added Stir an appropriate amount of honey into a paste, and put it into a granulator to granulate.

10. The Chinese medicine preparation for treating rabbit diarrhea according to any one of claims 1-4, wherein the dosage form of the Chinese medicine preparation is granule, powder, plaster or ointment.