CN103006460A - Skin-caring composition and preparation method and applications thereof - Google Patents
Skin-caring composition and preparation method and applications thereof Download PDFInfo
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- CN103006460A CN103006460A CN2012105834354A CN201210583435A CN103006460A CN 103006460 A CN103006460 A CN 103006460A CN 2012105834354 A CN2012105834354 A CN 2012105834354A CN 201210583435 A CN201210583435 A CN 201210583435A CN 103006460 A CN103006460 A CN 103006460A
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Abstract
The invention discloses a skin-caring composition and a preparation method and applications thereof. The skin-caring composition comprises 0.01-1 mg/ml tremella polysaccharide, 1-100 microgram/ml cell growth factors and a solvent for preparing each raw material solution with predetermined content. According to the skin-caring composition and the preparation method and applications thereof, the tremella polysaccharide and the cell growth factors are synergistically made into the skin-caring composition and preparation, the tremella polysaccharide and the cell growth factors are mutually complemented in function, the using effects of the two matters are larger than the sum of individual using effects due to the synergistic effect, so that unexpected effect is obtained in skin maintenance. The skin-caring composition provided by the invention not only can be used for daily skin maintenance, such as removing skin wrinkles, increasing skin elasticity and sheen, but also can be used for sensitive skin maintenance; and simultaneously, the skin-caring composition also can be used for recovering wound, removing scars and beautifying skin after the wound is healed or minimally invasive cosmetic surgery is performed.
Description
Technical field
The present invention relates to the skin care field, especially relate to a kind of skin care composition and method of making the same and application.
Background technology
People's aging comprises that skin aging was a kind of irreversible natural phenomena originally.Because the impact of the nervous factors such as life and work rhythm, environmental pollution and unsound living habit, this aging (at first showing as the aging of skin) phenomenon usually can occur ahead of time.Be embodied in wrinkle, the colour of skin inhomogeneous, coarse, lack flexibility, the so-called subhealth state skin such as the gloomy jaundice of the colour of skin, mottle freckle, drying, corse sweat pore.1997, a investigation report of World Health Organization (WHO) (WHO) showed: in the yellow, skin of face be in health status less than 10%, what be in morbid state surpasses 20%, and the skin that is in sub-health state accounts for 70% unexpectedly.
From biomedical angle, " subhealth state skin " is that various factors causes skin histology underactivity, is the class skin quality of boundary between health and morbid state.With advancing age, the fissionable skin progenitor cell number of skin basal layer gradually reduces, and the speed of skin renewal slows down; The metabolic activity of each level cell of skin reduces, and various albumen metabolic capacity synthetic and skin pigment descends.From external imagery, it show dark and gloomy simple and unadorned, with pigmentation, cutis laxa, wrinkle are deepened.Aging can't reverse, but can delay.For these years people expectation, by every possible means seek can slow down aging miraculous cure, but produce little effect so far.
Summary of the invention
The object of the invention is, provides a kind of skin care composition and method of making the same and application, with maintaining skin.
In a kind of embodiment in the present invention, provide a kind of skin care compositions, comprised tremella polysaccharide and the cell growth factor of 1～100 μ g/ml and the solvent that each raw material is assigned to predetermined content of 0.01～1mg/ml in this skin care compositions.
Employed tremella polysaccharide is the natural biological macromole that is extracted by Tremella among the present invention, have good biocompatibility, nontoxic, do not have immunogenicity, more stable than hyaluronic acid.Tremella polysaccharide is to be present in inside and outside the Tremella fructification born of the same parents and the macromolecular compound in the sporinite, has defying age, antioxidation and antineoplastic action.Tremella polysaccharide has stronger moisture-keeping functions than hyaluronic acid, and its moisture-retaining capacity is 1000 times of own wt.
Cell growth factor described in the present invention is the cell growth factor that the propagation of mice 3T3 cell is had obvious dose-dependence, comprising but be not limited to epidermal growth factor (EGF), acidity or basic fibroblast growth factor (a/bFGF) and keratinocyte growth factor (KGF) etc., these cell growth factor are the important cell growth regulators that contain in the Healthy People body fluid, has the widely active function with strengthening cell metabolic activity of promoting growth of cell, this cell growth factor can be induced the contrary skin progenitor cell that is divided into of Skin Cell simultaneously, but increase the number of skin basal layer somatoblast, accelerate the renewal speed of skin, but on clinical medicine accelerated wound healing, improve healing quality.
In the present invention with cell growth factor and the collaborative this skin care compositions of making of tremella polysaccharide, cell growth factor and tremella polysaccharide can mutually replenish on function, bring out the best in each other, the synergism of this two classes material make its effect that is used in conjunction with greater than its use separately add and effect, aspect maintaining skin, have unexpected effect.Skin care compositions of the present invention not only can be used for carrying out daily skin nursing, for example dispels wrinkle of skin, speckle, increases skin elasticity and gloss, can be used for being engaged in for a long time the people of outside work and has obvious sub-health population; Can also be used for the maintenance of sensitive skin; Simultaneously, after this skin care compositions also can be used for wound healing, Wicresoft's beautifying hand postoperative wound recovers, and removes cicatrix, beautifies skin.
Preferably, the cell growth factor that comprises tremella polysaccharide and 2～50 μ g/ml of 0.05～0.8mg/ml in the above-mentioned skin care compositions; More preferably, the cell growth factor that comprises tremella polysaccharide and 4～20 μ g/ml of 0.1～0.6mg/ml in the described skin care compositions, further preferably, the cell growth factor that comprises tremella polysaccharide and 10～15 μ g/ml of 0.1～0.5mg/ml in the described skin care compositions.
Preferably, also comprise food antiseptics 0.01～0.12m g/ml in the above-mentioned skin care compositions; Preferably, described food antiseptics is propylparaben and/or butoben, and more preferably, described food antiseptics is propylparaben 0.02～0.08mg/ml and butoben 0.02～0.08mg/ml.
Preferably, cell growth factor is selected from one or more in the group that is comprised of epidermal growth factor, acidity or basic fibroblast growth factor, keratinocyte growth factor in the above-mentioned skin care compositions, preferably, described cell growth factor is natural extract, artificial chemistry cell growth factor synthetic, source of human stem cell, that make by gene recombination technology or the derivant of described cell growth factor.
Preferably, tremella polysaccharide is natural extract or the tremella polysaccharide made by microbial fermentation in the above-mentioned skin care compositions, preferred bases dissolubility tremella polysaccharide, and its molecular weight is between 8～350kd.
Simultaneously, in one embodiment of the invention, a kind of preparation method of above-mentioned skin care compositions also is provided, has may further comprise the steps: preparation alkali solubility tremella polysaccharide content is 0.01～1mg/ml, and electric conductance is the alkali solubility tremella polysaccharide ultrafiltrate of 0～1mS/cm; Alkali solubility tremella polysaccharide ultrafiltrate mixed with optional food antiseptics behind autoclaving, be cooled to room temperature acquisition mixture A; To join among the mixture A after the cell growth factor filtration sterilization, stir and namely get the skin care compositions.
This preparation method provided by the present invention, by with tremella polysaccharide and cell growth factor respectively through hyperfiltration treatment, then merge through stirring, in the skin care compositions that obtains tremella polysaccharide and cell growth factor mix more even, in use better synergism and then raising skin care effect.
Preferably, the step of the described alkali solubility tremella polysaccharide ultrafiltrate of preparation comprises in the preparation method of above-mentioned skin care compositions: it is in 10～12 the pure water that dry tremella is joined pH in proportion, 100～140 ℃ of high temperature steamings 1～3 hour, mesh screen filters, get coarse filtration liquid and regulate pH to 6.8～7.2, with the doughnut membrane microfiltration of 0.4～0.5 μ m, get the clarification micro-filtrate; With the described micro-filtrate molecular cut off doughnut membrane ultrafiltration that is 250～350kd, get the first ultrafiltrate, with the doughnut membrane ultrafiltration of described the first ultrafiltrate with 8～12kd, with pure water repeatedly eluriate be 0～1mS/cm to electric conductance after, continue to be concentrated into tremella polysaccharide concentration between 0.01～1g/1000ml.
Preferably, doing filtration sterilization after the described cell growth factor of membrane filtration of employing 0.22 μ m in the preparation method of above-mentioned skin care compositions processes.
The prepared this skin care compositions of the present invention can be made into the skin care preparation.Wherein preferred skin care preparation is for after being used for maintaining daily skin, wound healing, after Wicresoft's cosmetic surgery and the preparation of allergic skin, and preferably, the cosmetic surgery of described Wicresoft is micropin or laser surgery.
Preferably, this skin care preparation provided by the present invention is liquid preparation, lyophilized formulations, gel or is adsorbed on and makes facial film or pad pasting on the inert solid support, preferably, inert solid support is selected from any in non-woven fabrics, filter paper, silk fabric, the bacterial fibers film.Preferably, when this skin care preparation is gel, can add 0.02～0.1% Acritamer 940.
The above-mentioned skin care compositions skin surface that spreads upon wish maintenance gets final product in the present invention.Preferably a kind of, effect preferably method may further comprise the steps: (1) thoroughly cleaning is facial; (2) carry out the position that maintenance processes at needs and drip skin care compositions of the present invention, consumption can be 0.05～0.5ml/cm
2(3) process in the position row micropin maintenance that is coated with skin care compositions of the present invention, available electronic micro-needle cosmetic instrument is processed in the micropin maintenance, and (for example Korea S produces, model MCURE) carries out skin care, regulate the micropin degree of depth 0.25～2mm, frequency of vibration per minute 2000 times, till absorbing fully to the skin care compositions; (4) after the skin care compositions absorbs fully, apply the facial film (can be positioned in the refrigerator before this facial film use and lower the temperature) that contains skin care compositions of the present invention, take off after 15～20 minutes, need not washing.Weekly did 1 micropin and process at 15～30 days a course for the treatment of of this skin care compositions provided by the present invention, and the maintenance of finishing a course for the treatment of can obtain preferably skin effects.
In the present invention with cell growth factor and collaborative this skin care compositions and the preparation of making of tremella polysaccharide, cell growth factor and tremella polysaccharide can mutually replenish on function, bring out the best in each other, the synergism of this two classes material make its effect that is used in conjunction with greater than its use separately add and effect, aspect maintaining skin, have unexpected effect.Skin care compositions of the present invention not only can be used for carrying out daily skin nursing, for example dispels wrinkle of skin, increases skin elasticity and gloss, can be used for being engaged in for a long time the people of outside work and has obvious sub-health population; Can also be used for the maintenance of sensitive skin; Simultaneously, after this skin care compositions also can be used for wound healing, Wicresoft's beautifying hand postoperative wound recovers, and removes cicatrix, beautifies skin.
Except purpose described above, feature and advantage, the present invention also has other purpose, feature and advantage.The below is with reference to the specific embodiment, and the present invention is further detailed explanation.
The specific embodiment
Below in conjunction with specific embodiment embodiments of the invention are elaborated, but following embodiment and accompanying drawing only are to understand the present invention, and can not limit the present invention, the multitude of different ways that the present invention can be defined by the claims and cover is implemented.
Further specify beneficial effect of the present invention below with reference to embodiment 1-8 and Comparative Examples 1-3.
Embodiment 1:
Raw material: contain in 1000ml: growth factor-21 mg (epidermal growth factor (EGF)), Tremella 3.5.g and an amount of distilled water.
Preparation method:
(1) dry tremella being joined in proportion 1000mlpH is in 10 the pure water, 100 ℃ of high temperature steamings 3 hours, and mesh screen filters, and gets coarse filtration liquid and regulates pH to 6.8, with the doughnut membrane microfiltration of 0.4 μ m, gets the clarification micro-filtrate; With the described micro-filtrate molecular cut off doughnut membrane ultrafiltration that is 250kd, get the first ultrafiltrate, with described the first ultrafiltrate doughnut membrane ultrafiltration with 8kd, with pure water repeatedly eluriate be 0.1mS/cm to electrical conductivity after, continue to be concentrated into tremella polysaccharide concentration at 1g/1000ml;
(2) with described alkali solubility tremella polysaccharide ultrafiltrate through 121 ℃, 1.1kg/cm
2Be cooled to room temperature behind the high pressure steam sterilization and obtain mixture A; (3) join among the described mixture A after cell growth factor being adopted the membrane filtration degerming of 0.22 μ m, stir and namely get described skin care compositions.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415; Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 1mg/ml and cell growth factor and the distilled water of 1 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 8～250kd.
Embodiment 2:
Raw material: contain in 1000ml: cell growth factor 10 0mg (wherein; Epidermal growth factor (EGF) 50mg, fibroblast growth factor (FGF) 25mg, keratinocyte growth factor (KGF) 25mg), Tremella 0.035g, butoben 0.1g, and an amount of distilled water.
Preparation method:
(1) dry tremella being joined in proportion 1000ml pH is in 12 the pure water, 140 ℃ of high temperature steamings 1 hour, and mesh screen filters, and gets coarse filtration liquid and regulates pH to 7, with the doughnut membrane microfiltration of 0.45 μ m, gets the clarification micro-filtrate; With the described micro-filtrate molecular cut off doughnut membrane ultrafiltration that is 350kd, get the first ultrafiltrate, with described the first ultrafiltrate doughnut membrane ultrafiltration with 12kd, with pure water repeatedly eluriate be 0.1mS/cm to electrical conductivity after, continue to be concentrated into tremella polysaccharide concentration at 0.01g/1000ml;
(2) with described alkali solubility tremella polysaccharide ultrafiltrate through 121 ℃, 1.1kg/cm
2Be cooled to room temperature behind the high pressure steam sterilization and obtain mixture A;
(3) join among the described mixture A after cell growth factor being adopted the membrane filtration degerming of 0.22 μ m, stir and namely get described skin care compositions.、
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415 Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.01mg/ml and cell growth factor and the distilled water of 1 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 12～350kd.
Embodiment 3:
Raw material: contain in 1000ml: cell growth factor 2mg (wherein; Epidermal growth factor (EGF) 1mg, fibroblast growth factor (FGF) 0.5mg, keratinocyte growth factor (KGF) 0.5mg), Tremella 2.8g, propylparaben 2mg, butoben 8mg, and an amount of distilled water.
(1) dry tremella being joined in proportion 1000ml pH is in 10 the pure water, 100 ℃ of high temperature steamings 1 hour, and mesh screen filters, and gets coarse filtration liquid and regulates pH to 6.8, with the doughnut membrane microfiltration of 0.4 μ m, gets the clarification micro-filtrate; With the described micro-filtrate molecular cut off doughnut membrane ultrafiltration that is 250kd, get the first ultrafiltrate, with described the first ultrafiltrate doughnut membrane ultrafiltration with 8kd, with pure water repeatedly eluriate be 0～1mS/cm to electrical conductivity after, continue to be concentrated into tremella polysaccharide concentration at 0.8mg/ml;
(2) described alkali solubility tremella polysaccharide ultrafiltrate is mixed with optional food antiseptics behind autoclaving, be cooled to room temperature acquisition mixture A;
(3) join among the described mixture A after cell growth factor being adopted the membrane filtration degerming of 0.22 μ m, stir and namely get described skin care compositions.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415 Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.8mg/ml and cell growth factor, 2mg/ml propylparaben, 8mg/ml butoben and the distilled water of 2 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 8～250kd.
Embodiment 4:
Raw material: contain in 1000ml: cell growth factor 10 mg (wherein; Epidermal growth factor (EGF) 5mg, fibroblast growth factor (FGF) 2.5mg, keratinocyte growth factor (KGF) 2.5mg), Tremella 0.175g, propylparaben 8mg, butoben 2mg, and an amount of distilled water.
(1) dry tremella being joined in proportion 1000ml pH is in 10 the pure water, 121 ℃ of high temperature steamings 1 hour, and mesh screen filters, and gets coarse filtration liquid and regulates pH to 7.2, with the doughnut membrane microfiltration of 0.45 μ m, gets the clarification micro-filtrate; With the described micro-filtrate molecular cut off doughnut membrane ultrafiltration that is 300kd, get the first ultrafiltrate, with described the first ultrafiltrate doughnut membrane ultrafiltration with 8kd, with pure water repeatedly eluriate be 0.1mS/cm to electrical conductivity after, continue to be concentrated into tremella polysaccharide concentration at 0.05g/1000ml;
(2) described alkali solubility tremella polysaccharide ultrafiltrate is mixed with optional food antiseptics behind autoclaving, be cooled to room temperature acquisition mixture A;
(3) join among the described mixture A after cell growth factor being adopted the membrane filtration degerming of 0.22 μ m, stir and namely get described skin care compositions.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A490*0.009415 in the sample Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.05mg/ml and cell growth factor, 8mg/ml propylparaben, 2mg/ml butoben and the distilled water of 15 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 8～300kd.
Embodiment 5:
Raw material: contain in 1000ml: cell growth factor 4mg (wherein; Epidermal growth factor (EGF) 2mg, fibroblast growth factor (FGF) 1mg, keratinocyte growth factor (KGF) 1mg), Tremella 2.1g, propylparaben 0.05g, butoben 0.05g, and an amount of distilled water.
Preparation method: with embodiment 3.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415; Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.6mg/ml and cell growth factor, 5mg/ml propylparaben, 5mg/ml butoben and the distilled water of 4 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 8～250kd.
Embodiment 6:
Raw material: contain in 1000ml: cell growth factor 20mg (wherein; Epidermal growth factor (EGF) 10mg, fibroblast growth factor (FGF) 5mg, keratinocyte growth factor (KGF) 5mg), Tremella 2.1g, propylparaben 0.05g, butoben 0.05g, and an amount of distilled water.
Preparation method: with embodiment 4.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415 Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.6mg/ml and cell growth factor, 5mg/ml propylparaben, 5mg/ml butoben and the distilled water of 20 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 8～300kd.
Embodiment 7:
Raw material: contain in 1000ml: growth factor-21 5mg (wherein; Epidermal growth factor (EGF) 10mg, fibroblast growth factor (FGF) 2.5mg, keratinocyte growth factor (KGF) 2.5mg), Tremella 1.4.g, propylparaben 0.05g, butoben 0.05g, and an amount of distilled water.
Preparation method: with embodiment 3.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415; Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain in the prepared skin care compositions after testing the tremella polysaccharide of 0.4mg/ml and 15 μ g/ml cell growth factor, 5mg/ml propylparaben, 5mg/ml butoben and distilled water, wherein the tremella polysaccharide molecular weight is between 8～250kd.
Embodiment 8:
Raw material: contain in 1000ml: growth factor-21 5mg (wherein; Epidermal growth factor (EGF) 10mg, fibroblast growth factor (FGF) 2.5mg, keratinocyte growth factor (KGF) 2.5mg), Tremella 0.35g propylparaben 0.05g, butoben 0.05g, and an amount of distilled water.
Preparation method: with embodiment 4.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415; Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.1mg/ml and cell growth factor, 5mg/ml propylparaben, 5mg/ml butoben and the distilled water of 15 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 8～300kd.
Comparative Examples 1:
Raw material: contain in 1000ml:
Cell growth factor 10 0mg (wherein; Epidermal growth factor (EGF) 50mg, fibroblast growth factor (FGF) 25mg, keratinocyte growth factor (KGF) 25mg), butoben 0.1g, and distilled water.
Preparation method: with embodiment 2, in (1) and (2).
Product content detects: growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260 contains cell growth factor and the distilled water of 100 μ g/ml after testing in the prepared skin care compositions.
Comparative Examples 2:
Raw material: contain in 1000ml: the distilled water of Tremella 3.5g and surplus, press embodiment 2 preparation tremella polysaccharides, membrance concentration to tremella polysaccharide content is 1mg/ml, stirs and namely gets described skin care compositions.
Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415, contain after testing tremella polysaccharide and the distilled water of 1mg/ml in the prepared skin care compositions.
Comparative Examples 3:
Raw material: contain in 1000ml: growth factor-21 10mg (wherein; Epidermal growth factor (EGF) 60mg, fibroblast growth factor (FGF) 25mg, keratinocyte growth factor (KGF) 25mg), Tremella 0.035g, propylparaben 0.05g, butoben 0.05g, butoben 0.1g, and the distilled water of surplus.
Preparation method: with embodiment 2.Product content detects: tremella polysaccharide phenol sulfuric acid colorimetric method for determining, in the 1ml sample liquid, add 1ml phenol reagent and 5ml concentrated sulphuric acid, and shake up, room temperature was placed 30 minutes, measured A at wavelength 490nm
490Tremella polysaccharide content (mg/ml)=A in the sample
490* 0.009415 Growth factor content is measured with ultraviolet absorption method, growth factor content (mg/ml)=1.55*Abs280-0.76*Abs260, contain after testing the tremella polysaccharide of 0.01mg/ml and cell growth factor and the distilled water of 110 μ g/ml in the prepared skin care compositions, wherein the tremella polysaccharide molecular weight is between 12～350kd.
One. stability test
Prepared drug sample carries out respectively following test among employing claim 1-8 and the Comparative Examples 1-3:
Method of testing: under normal temperature condition, the interior maintenance in time limit of observing 24 months, stablize, and the results are shown in Table 1 with prepared skin care compositions.
Table 1. formulation stability is observed, MAIN OUTCOME MEASURES: whether solution is muddy, the appearance precipitation
| Embodiment | 1 | 3 | 6 | 12 | 24 |
| Embodiment 1 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 2 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 3 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 4 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 5 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 6 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 7 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Embodiment 8 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Comparative Examples 1 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Comparative Examples 2 | Clarification | Clarification | Clarification | Clarification | Clarification |
| Comparative Examples 3 | Clarification | Clarification | Clarification | Clarification | Clarification |
By content in the table 1 as can be known, having good stability of skin care combination provided by the present invention, and use the prepared skin care compositions of above-described embodiment after 24 months, effective maintaining skin still, this shows, the effective life of skin care compositions provided by the present invention, can reach 24 months at least, even longer.
Two, effective percentage test
Method of testing: seeking respectively facial skin has wrinkle, mottle, and the experimenter who leaves Wicresoft, the rear cicatrix of operation on the skin.The experimenter is divided into 11 groups, carries out from the body controlled trial with the skin care compositions that contains above-mentioned 11 compound compositions respectively, observe it to improving the appearance effect of face's wrinkle and gloss:
The using method of skin care compositions of the present invention is as follows: (1) thoroughly cleaning is facial; (2) carry out the position that maintenance processes at needs and drip skin care compositions of the present invention, consumption can be 0.1ml/cm
2(3) (for example Korea S produces with electronic micro-needle cosmetic instrument, model MCURE), regulate micropin degree of depth 0.25mm, frequency of vibration per minute 2000 times, process in the position row maintenance that is coated with skin care compositions of the present invention, till absorbing fully to the skin care compositions; (3) after the skin care compositions absorbs fully, apply the facial film (can be positioned in the refrigerator before this facial film use and lower the temperature) that contains skin care compositions of the present invention, take off after 15～20 minutes, need not washing.
Test result: according to asking 15 days that smear skin care compositions of the present invention, effective percentage is summarized as follows table 1.
Table 1. Wicresoft Precerving liquid is to improving the effect of face's wrinkle and gloss
Annotate: refer to the skin after burn and scald heals more
By content in the table 1 as can be known, the effective percentage of the prepared skin care compositions of the present invention is apparently higher than the prepared skin care compositions of Comparative Examples 1-3.Simultaneously, by optimizing the content of tremella polysaccharide and cell growth factor in the prepared skin care compositions, but by both synergism Effective Raise skin protection effective percentage.
Three, Clinic Case
By data in the table 1 as can be known, the maintenance effect of the skin care compositions that embodiment of the invention 1-8 is prepared is all better, and wherein obviously to be better than embodiment 1 suitable with 2 effects with 2, embodiment 1 for embodiment 3-8.In the present invention in order to save the length of application documents.Below will be only in conjunction with the embodiments in 8 the skin care compositions carry out clinical experiment.
Trial volunteer: the age, there were obvious wrinkle, dark and gloomy lacklustre people in face from 35～65 years old men and women.
The using method of skin care compositions of the present invention is as follows: (1) thoroughly cleaning is facial; (2) carry out the position that maintenance processes at needs and drip skin care compositions of the present invention, consumption can be 0.1ml/cm
2(3) (for example Korea S produces with electronic micro-needle cosmetic instrument, model MCURE), regulate micropin degree of depth 0.25mm, frequency of vibration per minute 2000 times, process in the position row micropin maintenance that is coated with skin care compositions of the present invention, till absorbing fully to the skin care compositions; (3) after the skin care compositions absorbs fully, apply the facial film (can be positioned in the refrigerator before this facial film use and lower the temperature) that contains skin care compositions of the present invention, take off after 15～20 minutes, need not washing, massage out to the greatest extent elite Liquid Absorption.
Application example 1
Soup so-and-so, woman, 38 years old, certain company clerk.There is obvious crows-feet at the canthus, both sides, and the colour of skin is dim.The prepared skin care compositions-treated of operational approach one skidding embodiment 1 of guiding by the present invention, opposite side is done from the body controlled observation.After 3 days, process a side wrinkle through maintenance and shoal, both sides contrasts obviously, ruddy, the high resilience of skin after 15 days.
Application example 2
What so-and-so, woman, 56 years old, certain city civil servant.Xerosis cutis shows slightly lax, processes by guide row of the present invention maintenance, and after 13 days, skin is plentiful, high resilience and gloss.
Application example 3
Zhu, the woman, 47 years old, certain city civil servant, palor is dark and gloomy, matt, and there is obvious crows-feet at the canthus, both sides.Process by guide row maintenance of the present invention, the both sides crows-feet obviously shoals after 14 days, and cross-reference is obvious, the ruddy high resilience of skin.
Application example 4
Tang so-and-so, woman, 39 years old, certain programmer of computer company.The decree stricture of vagina on both sides is obvious, guides capable maintenance to process and maintenance by the present invention, and the decree stricture of vagina disappears substantially after one month.
Application example 5
Deng, woman, 46 years old, certain Top Management.Two cheeks have obvious mottle, guide capable maintenance to process by the present invention, 1 time weekly, apply ointment or plaster skin-care facial mask of the present invention after the processing after 20～30 minutes, hemorrhage and red and swollen disappearance.After this smear the prepared skin care compositions of the embodiment of the invention 1 every day and apply ointment or plaster by the skin-care facial mask of the prepared skin care compositions preparation of embodiment 1, after one month, mottle disappears substantially.
Application example 6
Zhang, woman, 45 years old, certain manager of pharmaceuticals.Severe allergy skin, the colour of skin is pale, apply ointment or plaster by the facial film of the prepared skin care compositions preparation of embodiment 1, every day 1 note, the irritated disappearance is of pink and healthy-looking after 7 days.
Application example 7
Grandson, woman, 38 years old, certain teacher of colleges and universities.The pox speckle is obvious on the face, guides capable maintenance to process by the present invention, 1 time weekly, applies ointment or plaster skin-care facial mask by the prepared skin care compositions preparation of embodiment 1 after maintenance is processed after 20～30 minutes, hemorrhage and red and swollen disappearance.After this smear the prepared skin care compositions of embodiments of the invention 1 every day and apply ointment or plaster by the facial film of the prepared skin care compositions preparation of embodiment 1, after one month, the pox speckle disappears substantially.
Application example 8
Chen, man, 22 years old, certain senior of colleges and universities.Skin is seriously tanned severely after beach swimming in the summer swimming, smears of the present inventionly by the prepared skin care compositions of embodiment 1, and applies ointment or plaster by the pad pasting of the prepared skin care compositions preparation of embodiment 1, and burning sensation goes down after 30 minutes, the red and swollen disappearance.
Application example 9
Hu, the woman, 48 years old, certain Top Management belonged to severe allergy skin, postoperative is processed in the row maintenance, apply the facial film that is prepared by embodiment 1 prepared skin care compositions, the petechia disappears substantially after 30 minutes, and obvious allergic phenomena does not occur, smear every day later on of the present inventionly by the prepared skin care compositions of embodiment 1, skin recovers normal fully after a couple of days.
Application example 10
Huang, 65 years old, the retired old man, cutis laxa, wrinkle is obvious.Process by the method row maintenance that the present invention guides, wrinkle shoals after 10 days, and wrinkle disappears substantially after 1 month.
By content among the above-mentioned application example 1-10 as can be known, the prepared this skin care composition effect of the present invention is obvious, can effectively improve the skin present situation, not only is fit to normal skin, also is applicable to simultaneously allergic skin.
Be the preferred embodiments of the present invention only below, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a skin care compositions is characterized in that, comprises tremella polysaccharide and the cell growth factor of 1～100 μ g/ml and the solvent that each raw material is assigned to predetermined content of 0.01～1mg/ml in the described skin care compositions.
2. skin care compositions according to claim 1 is characterized in that, comprises the cell growth factor of tremella polysaccharide and 2～50 μ g/ml of 0.05～0.8mg/ml in the described skin care compositions; Preferably, the cell growth factor that comprises tremella polysaccharide and 4～20 μ g/ml of 0.1～0.6mg/ml in the described skin care compositions, more preferably, the cell growth factor that comprises tremella polysaccharide and 10～15 μ g/ml of 0.1～0.5mg/ml in the described skin care compositions.
3. skin care compositions according to claim 1 is characterized in that, described skin care compositions also comprises food antiseptics 0.01～0.12mg/ml; Preferably, described food antiseptics is propylparaben and/or butoben, and more preferably, described food antiseptics is propylparaben 0.02～0.08m g/ml and butoben 0.02～0.08mg/ml.
4. skin care compositions according to claim 1, it is characterized in that, described cell growth factor is selected from by epidermal growth factor, acidity or basic fibroblast growth factor, in the group that keratinocyte growth factor forms one or more, preferably, described cell growth factor is natural extract, artificial chemistry synthesizes, source of human stem cell, by the cell growth factor of gene recombination technology manufacturing or the derivant of described cell growth factor, preferably, described tremella polysaccharide is natural extract, or the tremella polysaccharide of making by microbial fermentation, preferred described tremella polysaccharide molecular weight is between 8～350kd.
5. the preparation method of each described skin care compositions in the claim 1 to 4 is characterized in that, may further comprise the steps:
Preparation alkali solubility tremella polysaccharide content is 0.01～1mg/ml, and electrical conductivity is the alkali solubility tremella polysaccharide ultrafiltrate of 0～1mS/cm;
Described alkali solubility tremella polysaccharide ultrafiltrate mixed with optional food antiseptics behind autoclaving, be cooled to room temperature acquisition mixture A;
Namely get described skin care compositions with joining after the cell growth factor filtration sterilization among the described mixture A, stirring.
6. skin care compositions according to claim 5 is characterized in that, the step for preparing described alkali solubility tremella polysaccharide ultrafiltrate comprises:
It is in 10～12 the pure water that dry tremella is joined pH in proportion, 100～140 ℃ of high temperature steamings 1～3 hour, and mesh screen filters, and gets coarse filtration liquid and regulates pH to 6.8～7.2, with the doughnut membrane microfiltration of 0.4～0.5 μ m, gets the clarification micro-filtrate; With the described micro-filtrate molecular cut off doughnut membrane ultrafiltration that is 250～350kd, get the first ultrafiltrate, with the doughnut membrane ultrafiltration of described the first ultrafiltrate with 8～12kd, with pure water repeatedly eluriate be 0～1mS/cm to electrical conductivity after, continue to be concentrated into tremella polysaccharide concentration between 0.01～1g/1000ml.
7. skin care compositions according to claim 5 is characterized in that, adopts the described cell growth factor of membrane filtration of 0.22 μ m to do the filtration sterilization processing.
8. each described skin care compositions application in the skin care preparation in the claim 1 to 4.
9. application according to claim 8, it is characterized in that, described skin care preparation is for after being used for maintaining daily skin, wound healing, after Wicresoft's cosmetic surgery and the preparation of allergic skin, and preferably, the cosmetic surgery of described Wicresoft is micropin or laser beautifying operation.
10. application according to claim 8, it is characterized in that, described skin care preparation is liquid preparation, lyophilized formulations, gel or is adsorbed on and makes facial film or pad pasting on the inert solid support, preferably, described inert solid support is selected from any in non-woven fabrics, filter paper, silk fabric, the bacterial fibers film.
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| CN103409479A (en) * | 2013-08-23 | 2013-11-27 | 秘唐生物科技（上海）有限公司 | High polymer for enhancing natural water replenishing capability of skin and activating energy of cells and preparation method thereof |
| CN104800894A (en) * | 2015-04-30 | 2015-07-29 | 重庆市海洁消毒卫生用品有限责任公司 | Formula and preparation method of human lubricant |
| CN104815350A (en) * | 2015-04-16 | 2015-08-05 | 福州百正医药科技有限公司 | Tremella polysaccharide dressing and preparing method of product of tremella polysaccharide dressing |
| CN105708722A (en) * | 2016-03-15 | 2016-06-29 | 深圳市真兴病毒技术有限公司 | Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product |
| CN105708778A (en) * | 2016-02-02 | 2016-06-29 | 广州赛莱拉干细胞科技股份有限公司 | Skin care composition, skin cream and preparation method thereof of skin cream |
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| CN106110304A (en) * | 2016-07-28 | 2016-11-16 | 广州赛莱拉干细胞科技股份有限公司 | One is dispelled scar compositions and dressing |
| CN107308061A (en) * | 2017-07-31 | 2017-11-03 | 广州美中生物科技有限公司 | Repair composition, skin care item of skin and preparation method thereof |
| WO2018196109A1 (en) * | 2017-04-27 | 2018-11-01 | 苏州纳通生物纳米技术有限公司 | Application of micro-needle in skin cleaning |
| WO2024088060A1 (en) * | 2022-10-28 | 2024-05-02 | 上海家化联合股份有限公司 | Composition for regulating and controlling skin micro-ecological diversity |
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| CN104800894A (en) * | 2015-04-30 | 2015-07-29 | 重庆市海洁消毒卫生用品有限责任公司 | Formula and preparation method of human lubricant |
| CN105708778A (en) * | 2016-02-02 | 2016-06-29 | 广州赛莱拉干细胞科技股份有限公司 | Skin care composition, skin cream and preparation method thereof of skin cream |
| CN105708778B (en) * | 2016-02-02 | 2018-09-21 | 广州赛莱拉干细胞科技股份有限公司 | A kind of skin care compositions and a kind of face cream and preparation method thereof |
| CN105708722A (en) * | 2016-03-15 | 2016-06-29 | 深圳市真兴病毒技术有限公司 | Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product |
| CN106110303A (en) * | 2016-07-28 | 2016-11-16 | 广州赛莱拉干细胞科技股份有限公司 | One is dispelled scar compositions and dressing |
| CN106110304A (en) * | 2016-07-28 | 2016-11-16 | 广州赛莱拉干细胞科技股份有限公司 | One is dispelled scar compositions and dressing |
| WO2018196109A1 (en) * | 2017-04-27 | 2018-11-01 | 苏州纳通生物纳米技术有限公司 | Application of micro-needle in skin cleaning |
| CN107308061A (en) * | 2017-07-31 | 2017-11-03 | 广州美中生物科技有限公司 | Repair composition, skin care item of skin and preparation method thereof |
| WO2024088060A1 (en) * | 2022-10-28 | 2024-05-02 | 上海家化联合股份有限公司 | Composition for regulating and controlling skin micro-ecological diversity |
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