CN105708722A - Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product - Google Patents
Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product Download PDFInfo
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- CN105708722A CN105708722A CN201610147107.8A CN201610147107A CN105708722A CN 105708722 A CN105708722 A CN 105708722A CN 201610147107 A CN201610147107 A CN 201610147107A CN 105708722 A CN105708722 A CN 105708722A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention provides a factor composition and composite freeze-dried powder for promoting skin cell growth and a skin care product. The factor composition for promoting skin cell growth comprises EGF, bFGF and KGF; the composite freeze-dried powder for promoting skin cell growth is prepared from, by weight, 0.0003-0.0018% of the factor composition for promoting skin cell growth, 0.1-5% of asiaticoside, 5-28% of freeze-drying protecting agent and the balance water. By the adoption of the technical scheme, the three cell growth factors serve as ingredients for achieving main effects, and can achieve a synergistic effect to well promote growth of human body skin cells and improve the skin of people; through the freeze-drying process, bioactivity of the cell growth factors can be effectively guaranteed; the obtained skin care product has multiple effects and can comprehensively improve the skin of people.
Description
Technical field
The invention belongs to technical field of biological genetic engineering, particularly relate to and a kind of promote the factor composition of dermal cell growth, compound lyophilized powder and skin care item.
Background technology
In recent years, biology gene engineering technology obtains significant progress, directly influencing the every aspect of life sciences, all bring brand-new opportunity to develop also to cosmetics industry simultaneously, cosmetics develop from traditional synthesis cosmetics, natural cosmetics to biological gene cosmetics.In the biological gene cosmetics epoch, mainly using a series of cell growth factor as main composition, it is added directly to protective skin cream, milky lotion, eye cream, surfactant, facial film, repair in the cosmetics such as elite, to reach to repair the cosmetic results such as skin, crease-resistant, the light red blood streak.
And in the prior art, major part is single or the combination product of two kinds of cytokines, directly result in product efficacy comparatively single, it is impossible to the comprehensive skin improving people.Meanwhile, having biological activity cell growth factor owing to being added directly in the cosmetic base containing relatively complicated ingredient, so cannot ensure whether it there is also activity after mixing for a long time with cosmetic base, the effect of product is had a greatly reduced quality.
Summary of the invention
For above technical problem, the invention discloses and a kind of promote the factor composition of dermal cell growth, compound lyophilized powder and skin care item, there is the effect better promoting Growth of Cells, and the better biological activity keeping cell growth factor.
To this, the technical scheme is that
A kind of factor composition promoting dermal cell growth, including EGF(EpidermalGrowthFactor, epidermal growth factor), bFGF(basicFibroblastGrowthFactor, basic fibroblast growth factor) and KGF(KeratinocyteGrowthFactor, corneocyte somatomedin).
Wherein, EGF can promote reepithelialization, accelerates the synthesis rate of hyaluronic acid and glycoprotein, improves the microenvironment of Growth of Cells, and damaged skin, sensitive skin, trauma skin are had good restoration care effect by it, reduce the formation of cicatrix.BFGF can promote that fibroblastic growth promoter promotes collagen protein synthesis, repairs the collagen elastic fiber of aging fracture, thus preventing wrinkle to produce, dispel fine wrinkles, allows skin consolidation youth state.The biological functions such as KGF has promotion epithelial cell proliferation and differentiation, maintains cytoskeleton stable, protection radiation, strengthen skin barrier.This technical scheme considers the different biological natures of different cell growth factor, through many experiments, it has been found that using these three kinds of cell growth factor as the composition playing primary efficacy, its three can produce synergism each other, the growth of human skin cell can well be promoted, improve the skin of people.
As a further improvement on the present invention, described EGF, bFGF and KGF mass ratio be (1.5 ~ 2.5): (0.8 ~ 1.3): 1.
As a further improvement on the present invention, described EGF, bFGF and KGF mass ratio be 2:1:1.Adopt this technical scheme, there is the growth that better can well promote human skin cell, there is anti-anaphylaxis reparation, scar of dispelling anti-acne, anti-ageing wrinkle resistant, the multiple efficacies such as lifting of compacting, the omnibearing skin quality improving people。
The invention also discloses a kind of compound lyophilized powder promoting dermal cell growth; it includes promoting the factor composition of dermal cell growth, asiaticoside and freeze drying protectant as above; the percentage by weight of each component is: the described factor composition 0.0003 ~ 0.0018% promoting dermal cell growth; asiaticoside 0.1 ~ 5%; freeze drying protectant 5 ~ 28%, surplus is water.This technical scheme is by special frozen-dried protective agent prescription and lyophilizing technique; can effectively ensure that the biological activity of cell growth factor, and by three kinds of cell growth factor scientifically being arranged in pairs or groups in proportion, enable the various factor effectively complementary; synergism, more efficiently improves mankind's skin.Wherein, adopting low-temperature vacuum drying technology, make cell factor composition in room temperature long-term storage, can not easily lose biological activity, to guarantee in use, various cytokines play reparation effect of correspondence.
As a further improvement on the present invention, the percentage by weight of each component is: the described factor composition 0.0004 ~ 0.0016% promoting dermal cell growth, asiaticoside 1 ~ 4%, freeze drying protectant 7 ~ 25%, surplus is water.
As a further improvement on the present invention, described freeze drying protectant includes mannitol and trehalose, described mannitol and the trehalose percentage by weight in the compound lyophilized powder of described promotion dermal cell growth and is: mannitol 5 ~ 15%, trehalose 2 ~ 10%.
As a further improvement on the present invention, the described compound lyophilized powder promoting dermal cell growth adopts following steps to prepare:
Step S1: EGF stock solution, bFGF stock solution and KGF stock solution are mixed, and is added thereto to asiaticoside, obtain cytokine composite solution；
Step S2: configuration freeze drying protectant, and through membrane filtration, obtain freeze drying protectant coarse filtration solution；
Step S3: described cytokine composite solution and freeze drying protectant coarse filtration solution being mixed, add water constant volume, through filtering; it is filled in aseptic cillin bottle, partly detains plug with plug, in-60 DEG C to-20 DEG C frozen dryings; obtaining compound lyophilized powder, tamponade, gland obtains finished product.
As a further improvement on the present invention, in step S2, described filter membrane is 600 orders.
As a further improvement on the present invention, in step S3, through 0.22 μm of membrane filtration during filtration.
The invention also discloses a kind of skin care item promoting dermal cell growth, including the compound lyophilized powder and the solvent that promote dermal cell growth as above.Adopt this technical scheme, after lyophilized powder and solvent are mixed, coordinate micropin to use, there is the effect of the more omnibearing skin quality improving people.
As a further improvement on the present invention, component and percentage by weight thereof that described solvent comprises are: hyaluronic acid 0.02 ~ 0.1%, glycerol 3 ~ 8%, surplus is water.
Compared with prior art, the invention have the benefit that
First, adopt technical scheme, it is contemplated that the different biological natures of different cell growth factor, these three kinds of cell growth factor are as the composition playing primary efficacy, its three can produce synergism each other, it is possible to well promotes the growth of human skin cell, improves the skin of people.
Second; adopt technical scheme; by special frozen-dried protective agent prescription and lyophilizing technique; can effectively ensure that the biological activity of cell growth factor; and by three kinds of cell growth factor are scientifically arranged in pairs or groups in proportion; enable the various factor effectively complementary, synergism, more efficiently improves mankind's skin.
3rd, adopt the skin care item of technical scheme, there is anti-anaphylaxis reparation, scar of dispelling anti-acne, anti-ageing wrinkle resistant, the multiple efficacies such as lifting of compacting, can the omnibearing skin quality improving people。
Accompanying drawing explanation
Fig. 1 is the Cell proliferation results figure that the embodiment of the present invention 1 adds the factor composition 48h promoting dermal cell growth in NIH3T3 cell.
Fig. 2 is the Cell proliferation results figure that comparative example 1 of the present invention adds EGF factor 48h in NIH3T3 cell.
Fig. 3 is the Cell proliferation results figure that comparative example 2 of the present invention adds PBS48h in NIH3T3 cell.
Detailed description of the invention
Below the preferably embodiment of the present invention is described in further detail.
Embodiment 1
A kind of factor composition promoting dermal cell growth, including EGF, bFGF and KGF, described EGF, bFGF and KGF mass ratio be 2:1:1.EGF stock solution, bFGF stock solution and KGF stock solution are mixed according to above content ratio, obtains the factor composition of described promotion dermal cell growth.
Joining in NIH3T3 cell by the factor composition of the above-mentioned promotion dermal cell growth obtained, addition is observation of cell proliferative conditions after 1ng/ml, 48h, and result is as shown in Figure 1.
Comparative example 1
Adding the EGF factor in NIH3T3 cell, addition is observation of cell proliferative conditions after 1ng/ml, 48h, and result is as shown in Figure 2.
Comparative example 2
NIH3T3 cell adds PBS(phosphatebuffersaline, phosphate buffered saline(PBS)) as control sample, observation of cell proliferative conditions after 48h, result is as shown in Figure 3.
Being can be seen that by Fig. 1 ~ Fig. 3 contrast, comparative example 2 does not add the experiment of cytokine, and cell proliferation is slow；Comparative example 1 adds the sample of EGF, and cell has cultivation effect faster；And with the addition of the embodiment 1 comprising tri-kinds of cytokine samples of EGF, bFGF, KGF, greatly speed up than the above two cell proliferation rate；Fig. 1 and Fig. 2 contrasts visible, and under adding identical concentration, the cell proliferation rate of embodiment 1 is considerably beyond comparative example 1；Visible, play synergism between the cytokine of EGF, bFGF and KGF of embodiment 1, promoted that cell is bred faster, there is better effect.
Embodiment 2
A kind of compound lyophilized powder promoting dermal cell growth, the quality of its main component is: EGF0.04g, bFGF0.02g, KGF0.02g, asiaticoside 25g, mannitol 250g, trehalose 500g.
Prepared by employing following steps:
Step S1: EGF stock solution, bFGF stock solution and KGF stock solution are mixed, and is added thereto to asiaticoside, obtain cytokine composite solution；
Step S2: configuration freeze drying protectant, joins mannitol and trehalose in deionized water and mixes, and through 600 order membrane filtrations, obtain freeze drying protectant coarse filtration solution；
Step S3: described cytokine composite solution and freeze drying protectant coarse filtration solution are mixed; add water constant volume; degerming through 0.22 μm of membrane filtration, it is settled to 5000ml, in aseptic subpackaged 1000 bottles of cillin bottles; 5ml/ bottle; partly detain plug with plug, in-60 DEG C to-20 DEG C frozen dryings 20-40 hour, obtain compound lyophilized powder; tamponade, gland obtains finished product.
Compound lyophilized powder obtained above is adopted to be dissolved in solvent, wherein, solvent adopts following steps configuration: 1.5g hyaluronic acid, 150g glycerol are dissolved in filtration sterilization in 5000ml water for injection or deionized water, in aseptic subpackaged 1000 bottles of cillin bottles, obtain the solvent of 5ml/ bottle.
One bottle of compound lyophilized powder is dissolved in one bottle of solvent, is promoted the skin care item of dermal cell growth.
Embodiment 3
A kind of compound lyophilized powder promoting dermal cell growth, the quality of its main component is: EGF0.02g, bFGF0.009g, KGF0.011g, asiaticoside 50g, mannitol 500g, trehalose 250g.
Prepared by employing following steps:
Step S1: EGF stock solution, bFGF stock solution and KGF stock solution are mixed, and is added thereto to asiaticoside, obtain cytokine composite solution；
Step S2: configuration freeze drying protectant, joins mannitol and trehalose in deionized water and mixes, and through 600 order membrane filtrations, obtain freeze drying protectant coarse filtration solution；
Step S3: described cytokine composite solution and freeze drying protectant coarse filtration solution are mixed; add water constant volume; degerming through 0.22 μm of membrane filtration, it is settled to 5000ml, in aseptic subpackaged 1000 bottles of cillin bottles; 5ml/ bottle; partly detain plug with plug, in-60 DEG C to-20 DEG C frozen dryings 20-40 hour, obtain compound lyophilized powder; tamponade, gland obtains finished product.
Compound lyophilized powder obtained above is adopted to be dissolved in solvent, wherein, solvent adopts following steps configuration: 1.5g hyaluronic acid, 150g glycerol are dissolved in filtration sterilization in 5000ml water for injection or deionized water, in aseptic subpackaged 1000 bottles of cillin bottles, obtain the solvent of 5ml/ bottle.
One bottle of compound lyophilized powder is dissolved in one bottle of solvent, is promoted the skin care item of dermal cell growth.
Embodiment 4
A kind of compound lyophilized powder promoting dermal cell growth, the quality of its main component is: EGF0.01g, bFGF0.006g, KGF0.004g, asiaticoside 200g, mannitol 750g, trehalose 150g.
Prepared by employing following steps:
Step S1: EGF stock solution, bFGF stock solution and KGF stock solution are mixed, and is added thereto to asiaticoside, obtain cytokine composite solution；
Step S2: configuration freeze drying protectant, joins mannitol and trehalose in deionized water and mixes, and through 600 order membrane filtrations, obtain freeze drying protectant coarse filtration solution；
Step S3: described cytokine composite solution and freeze drying protectant coarse filtration solution are mixed; add water constant volume; degerming through 0.22 μm of membrane filtration, it is settled to 5000ml, in aseptic subpackaged 1000 bottles of cillin bottles; 5ml/ bottle; partly detain plug with plug, in-60 DEG C to-20 DEG C frozen dryings 20-40 hour, obtain compound lyophilized powder; tamponade, gland obtains finished product.
Compound lyophilized powder obtained above is adopted to be dissolved in solvent, wherein, solvent adopts following steps configuration: 1.5g hyaluronic acid, 150g glycerol are dissolved in filtration sterilization in 5000ml water for injection or deionized water, in aseptic subpackaged 1000 bottles of cillin bottles, obtain the solvent of 5ml/ bottle.
One bottle of compound lyophilized powder is dissolved in one bottle of solvent, is promoted the skin care item of dermal cell growth.
Through detection, the compound lyophilized powder promoting dermal cell growth of embodiment 2 ~ embodiment 4 meets the indices in " the cosmetics health specification 2007 " of country's promulgation, and said composition, without any noxious substance, has no side effect.The people that the product of the present invention exists various problem to skin being tried out, user is 100 people altogether, and after using at least 3 times, skin all improves significantly, and example 5 below ~ embodiment 7 is only several Application Example.
Embodiment 5
Liu, 45 years old, the crows-feet of face, wrinkles on one's forehead, decree stricture of vagina were obvious, and speckle occur in many places, and the colour of skin is dark yellow.Use the skin care item promoting dermal cell growth of embodiment 2, coordinate micropin to carry out facial-care, every two weeks 1 time, every time after nursing, also use the skin care item of the promotion dermal cell growth of embodiment 2 carry out for three days on end smear reparation.After the nursing of 3 times, the microgroove at canthus reduces a lot, and before deeper wrinkle is also desalinated, and the colour of skin has been absent from dark yellow phenomenon.
Embodiment 6
Chen, 38 years old, face's red blood streak was obvious, and sensitive skin, easy allergy, long-term face is general red.Use the skin care item promoting dermal cell growth of embodiment 3, coordinate micropin to carry out facial-care, every two weeks 1 time, nurse every time after, also use the product of the present invention carry out for three days on end smear reparation.After the nursing of 3 times, skin red blood streak be improved significantly, be continuing with after 5 times, red blood streak disappear.
Embodiment 7
Thanking certain, 18 years old, face, due to long comedo, corse sweat pore, especially position, T district, including forehead, nose, left deeper pox print cicatrix.Use the skin care item promoting dermal cell growth of embodiment 4, coordinate micropin to carry out facial-care, every two weeks 1 time, nurse every time after, also use the product of the present invention carry out for three days on end smear reparation.After the nursing of 3 times, skin be improved significantly, skin become compact, pox print cicatrix desalinate gradually, be continuing with after 5 times, skin becomes brilliant white, pox print cicatrix substantially smooth.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, it is impossible to assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, it is also possible to make some simple deduction or replace, protection scope of the present invention all should be considered as belonging to.
Claims (10)
1. the factor composition promoting dermal cell growth, it is characterised in that: include EGF, bFGF and KGF.
2. the factor composition of promotion dermal cell growth according to claim 1, it is characterised in that: described EGF, bFGF and KGF mass ratio be (1.5 ~ 2.5): (0.8 ~ 1.3): 1.
3. the factor composition of promotion dermal cell growth according to claim 2, it is characterised in that: described EGF, bFGF and KGF mass ratio be 2:1:1.
4. the compound lyophilized powder promoting dermal cell growth; it is characterized in that: it includes the promotion factor composition of dermal cell growth as described in claim 1 ~ 3 any one, asiaticoside and freeze drying protectant; the percentage by weight of each component is: the described factor composition 0.0003 ~ 0.0018% promoting dermal cell growth; asiaticoside 0.1 ~ 5%; freeze drying protectant 5 ~ 28%, surplus is water.
5. the compound lyophilized powder of promotion dermal cell growth according to claim 4; it is characterized in that: the percentage by weight of each component is: the described factor composition 0.0004 ~ 0.0016% promoting dermal cell growth; asiaticoside 1 ~ 4%, freeze drying protectant 7 ~ 25%, surplus is water.
6. the compound lyophilized powder promoting dermal cell growth according to claim 4 or 5; it is characterized in that: described freeze drying protectant includes mannitol and trehalose; described mannitol and the trehalose percentage by weight in the compound lyophilized powder of described promotion dermal cell growth is: mannitol 5 ~ 15%, trehalose 2 ~ 10%.
7. the compound lyophilized powder of promotion dermal cell growth according to claim 6, it is characterised in that: adopt following steps to prepare:
Step S1: EGF stock solution, bFGF stock solution and KGF stock solution are mixed, and is added thereto to asiaticoside, obtain cytokine composite solution；
Step S2: configuration freeze drying protectant, and through membrane filtration, obtain freeze drying protectant coarse filtration solution；
Step S3: described cytokine composite solution and freeze drying protectant coarse filtration solution being mixed, add water constant volume, through filtering, is filled in aseptic cillin bottle, partly detains plug with plug, in-60 DEG C to-20 DEG C frozen dryings, obtain compound lyophilized powder.
8. the compound lyophilized powder of promotion dermal cell growth according to claim 7, it is characterised in that: in step S2, described filter membrane is 600 orders；In step S3, through 0.22 μm of membrane filtration during filtration.
9. the skin care item promoting dermal cell growth, it is characterised in that: include the compound lyophilized powder promoting dermal cell growth as described in claim 4 ~ 8 any one and solvent.
10. the skin care item of promotion dermal cell growth according to claim 9, it is characterised in that: component and percentage by weight thereof that described solvent comprises be: hyaluronic acid 0.02 ~ 0.1%, glycerol 3 ~ 8%, surplus is water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610147107.8A CN105708722A (en) | 2016-03-15 | 2016-03-15 | Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610147107.8A CN105708722A (en) | 2016-03-15 | 2016-03-15 | Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product |
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| CN105708722A true CN105708722A (en) | 2016-06-29 |
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| CN201610147107.8A Pending CN105708722A (en) | 2016-03-15 | 2016-03-15 | Factor composition and composite freeze-dried powder for promoting skin cell growth and skin care product |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375004A (en) * | 2017-07-20 | 2017-11-24 | 房俊英 | A kind of colored lyophilized powder producing method |
| CN108265023A (en) * | 2016-12-30 | 2018-07-10 | 深圳华大基因研究院 | A kind of enhancer of proliferation and its application |
| CN108379215A (en) * | 2018-03-30 | 2018-08-10 | 广州赛隽生物科技有限公司 | A kind of cosmetic composition for having effects that the freeze-drying compound powder of skin whitening and including the freeze-drying compound powder |
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| CN1561960A (en) * | 2004-04-02 | 2005-01-12 | 西南生物工程产业化中试基地有限公司 | Method for preparing biological beauty skin-care product containing keratin cell growth factor-2 and recombined human keratin cell growth factor-2 |
| CN101791283A (en) * | 2010-02-05 | 2010-08-04 | 张德文 | Cosmetic capable of eliminating striae gravidarum and scars |
| CN103006460A (en) * | 2012-12-31 | 2013-04-03 | 涂桂洪 | Skin-caring composition and preparation method and applications thereof |
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2016
- 2016-03-15 CN CN201610147107.8A patent/CN105708722A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1561960A (en) * | 2004-04-02 | 2005-01-12 | 西南生物工程产业化中试基地有限公司 | Method for preparing biological beauty skin-care product containing keratin cell growth factor-2 and recombined human keratin cell growth factor-2 |
| CN101791283A (en) * | 2010-02-05 | 2010-08-04 | 张德文 | Cosmetic capable of eliminating striae gravidarum and scars |
| CN103006460A (en) * | 2012-12-31 | 2013-04-03 | 涂桂洪 | Skin-caring composition and preparation method and applications thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108265023A (en) * | 2016-12-30 | 2018-07-10 | 深圳华大基因研究院 | A kind of enhancer of proliferation and its application |
| CN108265023B (en) * | 2016-12-30 | 2021-12-03 | 深圳华大生命科学研究院 | Proliferation promoter and application thereof |
| CN107375004A (en) * | 2017-07-20 | 2017-11-24 | 房俊英 | A kind of colored lyophilized powder producing method |
| CN108379215A (en) * | 2018-03-30 | 2018-08-10 | 广州赛隽生物科技有限公司 | A kind of cosmetic composition for having effects that the freeze-drying compound powder of skin whitening and including the freeze-drying compound powder |
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