CN103004696A - Method for inducing Dysmicoccus neobrevipes Beardsley infertility - Google Patents
Method for inducing Dysmicoccus neobrevipes Beardsley infertility Download PDFInfo
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- CN103004696A CN103004696A CN2011102849781A CN201110284978A CN103004696A CN 103004696 A CN103004696 A CN 103004696A CN 2011102849781 A CN2011102849781 A CN 2011102849781A CN 201110284978 A CN201110284978 A CN 201110284978A CN 103004696 A CN103004696 A CN 103004696A
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Abstract
The invention discloses a method for inducing Dysmicoccus neobrevipes Beardsley infertility. The method comprises the following step of utilizing Co60-gamma ray to perform radiation treatment for various insect states of Dysmicoccus neobrevipes Beardsley, wherein the purpose is to enable various insect states of the Dysmicoccus neobrevipes Beardsley to be unable to continue breeding and developing; the radiation with a dosage more than 80 Gy can induce 1-instar nymph to not to produce F1-generation nymph; the radiation with a dosage more than 100 Gy can induce the 2-instar nymph and 3-instar nymph to not to produce F1-generation nymph; the radiation with a dosage more than 150 Gy can induce imageo without eggs to not to produce F1-generation nymph; and the radiation with a dosage of 200 Gy can induce imageo with eggs to not to produce F1-genration nymph. The method for inducing the Dysmicoccus neobrevipes Beardsley infertile provided by the invention is low in radiation dosage and strong in practicability; the method can be practically used in insect irradiation quarantine treatment; and moreover, a new method for the quarantine treatment measure of the trade fruit carrying the insects is provided.
Description
Technical field
The present invention relates to a kind of sterile method of new pineapple ash mealybug of inducing.
Background technology
New pineapple ash mealybug (Dysmicoccus neobrevipes Beardsley) belongs to Homoptera, Coccoidea (Coccoidea), and Pseudococcidae (Pseudococcidae), grey mealybug belongs to (Dysmicoccus).This insect belongs to pest in " People's Republic of China (PRC) enter the territory plant quarantine pest register ", frequently intercepting and capturing in the fruit that enters the territory.This worm mainly is distributed in the torrid zone, and a small amount of record is arranged in the subtropical zone, distribution is arranged, such as Fiji, Jamaica, Malay Archipelago, Mexico, Micronesia, Philippine and TaiWan, China etc. in the countries and regions of growth bromelia.This worm is found in Changjiang County, Hainan Province fiber crops district in 1998 first at the quarantine pest that China belongs to new incoming.Qin Zhen waits by force people (2010) by its risk in China has been carried out qualitative and quantitative analysis, finds that this mealybug is the pest of highly dangerous, is 2.12 at the integrated risk value R of China.Therefore, should actively take capable plant quarantine measure, process etc. such as quarantine, stop that it imports into, field planting and diffusion.
Find in the banana that enters the territory that at present the quarantine processing method that new pineapple ash mealybug is taked is generally the methyl bromide fumigation processing, but the people such as Sung B.K. report, the discovery quarantine harmful organisms is taked the quarantine processing mode of methyl bromide fumigation in the inward banana of Korea S, but stifling to the certain harmful effect of banana quality generation.According to staff's introduction of being engaged in line quarantine, banana quality has been subject to impact behind the methyl bromide fumigation simultaneously.And radiation treatment is a kind of quarantine treatment technology that rises after methyl bromide fumigation is about to be eliminated, and fruit irradiation quarantine treatment technology is day by day ripe at present.Therefore, utilize Co
60-gamma-rays carries out radiation treatment to new pineapple ash mealybug, has established the accurate dosage of new each worm attitude steriliation by irradiation of pineapple ash mealybug, implements the irradiation quarantine processing Technical Reference is provided in the hope of find new pineapple ash mealybug in trade fruit for the port.
Summary of the invention
The purpose of this invention is to provide a kind of sterile method of new pineapple ash mealybug of inducing.
Technical scheme of the present invention is summarized as follows:
Intercept and capture new pineapple ash mealybug in August, 2010 from the saba that enters the territory, breed many generations with japanese squash at laboratory rearing, the raising condition is 25 ℃, 60-80%RH, 14: 10 photoperiods (L: D) h.This worm is ovoviviparity, belongs to parthenogenesis, and female worm has 4 worm attitudes, be respectively 1 age nymph, 2 age nymph, 3 age nymph, adult.
Inoculate gently first about 100 new pineapple ash mealybug adult at each japanese squash with soft little writing brush type brush, produce F1 nymph in 1 age of generation after 5 days until adult, remove adult, with these in 1 age nymph in the same lower raising that imposes a condition, wait to grow the worm attitude to needs, collect the mealybug of some to soft toilet paper with the little writing brush type brush of softness, wrap and be placed in the ventilative micro-wave oven lunch box, bring to the irradiation that cobalt source radiation place carries out 0Gy, 50Gy, 80Gy, 100Gy, 150Gy, a 200Gy6 dose gradient, 3 repetitions of every gradient.Returned to the same day behind the irradiation, and the mealybug behind the irradiation carefully moved to place under the original condition on the fresh japanese squash that does not infect insect raise, and each is isolated between processing, and observes weekly the breeding situation.Can whether the adult whether nymph of observing nymph lethality behind the irradiation and survival can grow adult lethality to next worm attitude, the irradiation and survival can produce F1 be continued breeding for nymph, F1 for nymph; Contrast is because breeding quantity is excessive, produces F1 and adopts the method for indirect estimation for nymph quantity, has the pumpkin of mealybug evenly to be divided into some parts infection, chooses at random 2-3 part counting, multiply by total umber after average to be the contrast breeding amount.The general raising of mealybug behind the irradiation stops after 2 months observing, at this moment the equal dead of mealybug behind the irradiation.
The present invention find the above dosage radiation of 80Gy can induce 1 age nymph do not produce F1 for nymph, the above dosage radiation of 100Gy can induce 2 ages, 3 age nymph do not produce F1 for nymph, the above dosage radiation of 150Gy can be induced and is not with the ovum adult not produce F1 for nymph, and the 200Gy radiation can induce band ovum adult not produce F1 for nymph.
Therefore, the present invention utilizes Co
60-gamma-rays induces new pineapple ash mealybug sterile, has established the radiation hardness worm attitude of new pineapple ash mealybug and sterile irradiation dose thereof, implements the irradiation quarantine processing Technical Reference is provided in the hope of finds new pineapple ash mealybug in trade fruit for the port.
The present invention has actively useful effect:
1. along with the increase of dosage, the ratio that each worm attitude continues to grow obviously reduces.
The present invention finds that irradiation is comparatively obvious to the breeding development impact of each worm attitude of new pineapple ash mealybug, and along with the increase of irradiation dose, and the ratio for next worm attitude of growing obviously reduces (see Table 1, table 2, table 3, table 4).
2. the radiation hardness worm attitude of new pineapple ash mealybug is band ovum adult.
Band ovum adult produces F1 for nymph behind 150Gy irradiation, other worm attitudes all do not have F1 for generation through 150Gy and above dosage irradiation, shows, the radiation hardness worm attitude of new pineapple ash mealybug is for being with ovum adult (seeing Table 3).
3.200Gy can stop new all worm attitudes of pineapple ash mealybug to produce F1 generation.
4. irradiation technique is practicable for the quarantine processing method of new pineapple ash mealybug.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of sterile method of new pineapple ash mealybug of inducing, comprise the steps (seeing Table 1): grow behind the 1 age nymph irradiation be 2 age nymph large percentage, contrast has 92% nymph continuation growth, 60% nymph continuation growth is arranged behind the 50Gy irradiation, 52.3% nymph continuation growth is arranged behind the 80Gy irradiation, 40.5% nymph continuation growth is arranged behind the 100Gy irradiation, 26.8% nymph continuation growth is arranged behind the 150Gy irradiation, 18.6% nymph continuation growth is arranged behind the 200Gy irradiation; Continue to grow to 3 age nymph and the ratio of adult reduce gradually along with the increase of dosage, contrast has 79.6% nymph finally to sprout wings into adult, 50Gy irradiation has 10% nymph to sprout wings into adult, 80Gy irradiation has 3.7% nymph to sprout wings into adult, 100Gy irradiation has 1.7% nymph to sprout wings into adult, 150Gy irradiation only has 0.89% nymph to sprout wings into adult, and the nymph behind the 200Gy irradiation finally can not turn into adult; Only the nymph behind the 50Gy irradiation finally has 2 F1 to produce for nymph, grows generation F2 generation but can not continue breeding, and other dosage irradiation does not all have F1 to produce for nymph.
Embodiment 2
A kind of sterile method of new pineapple ash mealybug of inducing comprises the steps (seeing Table 2): along with the increase of irradiation dose, 2,3 age nymphal development be that the ratio of adult reduces gradually.Contrast has 94.5% nymph can turn into adult; 50Gy irradiation has 49.7% nymph to turn into adult, produces F1 for 23 of nymphs; 80Gy irradiation has 39% nymph to turn into adult, produces F1 for 3 of nymphs; 100Gy, 150Gy and 200Gy irradiation have respectively 30.1%, 26.6% and 23.4% nymph to turn into adult, all do not have F1 to produce for nymph.
Embodiment 3
A kind of sterile method of new pineapple ash mealybug of inducing comprises the steps (seeing Table 3): irradiation is not on comparatively obvious with the impact of ovum adult breeding growth.Average every the adult of contrast produces 112 of F1 nymphs, has 97.1% nymph to turn into adult, and continues to grow breeding F2 generation; 50Gy irradiation adult produces F1 for 75 of nymphs, has 76% nymph to turn into adult, and the adult of emergence produces F2 for 29 of nymphs; 80Gy irradiation adult produces F1 for 40 of nymphs, has 65% nymph to turn into adult, and the adult of emergence can not produce F2 generation; 100Gy irradiation adult produces F1 for 29 of nymphs, and nymph finally can not turn into adult; Adult behind 150Gy and the 200Gy irradiation all can not produce F1 for nymph.
Embodiment 4
A kind of sterile method of new pineapple ash mealybug of inducing comprises the steps (seeing Table 4): irradiation on the impact of growing with the breeding of ovum adult on not similar with the impact of ovum adult.Average every the adult of contrast produces 107 of F1 nymphs, has 94.5% nymph to turn into adult, and continues to grow breeding F2 generation; 50Gy irradiation adult produces F1 for 82 of nymphs, has 76.8% nymph to turn into adult, and the adult of emergence produces F2 for 38 of nymphs; 80Gy irradiation adult produces F1 for 53 of nymphs, has 60.4% nymph to turn into adult, and the adult of emergence can not produce F2 generation; 100Gy and 150Gy irradiation adult produce respectively F1 for 27 of nymphs and 9, but can not continue to turn into adult; Adult behind the 200Gy irradiation all can not produce F1 for nymph.
Table 1 irradiation is on 1 impact that age, the nymph breeding was grown
Table 2 irradiation is on 2,3 impacts that age, the nymph breeding was grown
The impact of table 3 irradiation on not growing with the breeding of ovum adult
The impact of table 4 irradiation on growing with the breeding of ovum adult
Claims (6)
1. induce the sterile method of new pineapple ash mealybug for one kind, it is characterized in that comprising the steps: to utilize Co
60-gamma-rays carries out radiation treatment to new each worm attitude of pineapple ash mealybug, its objective is that making new each worm attitude of pineapple ash mealybug can not continue to grow produces F1 generation.
2.80Gy above dosage radiation can induce 1 age nymph do not produce F1 for nymph.
3.100Gy above dosage radiation can induce 2 ages, 3 age nymph do not produce F1 for nymph.
4.150Gy can inducing, above dosage radiation is not with the ovum adult not produce F1 for nymph.
5.200Gy radiation can induce band ovum adult not produce F1 for nymph.
6. the radiation hardness worm attitude of new pineapple ash mealybug is band ovum adult.
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| CN2011102849781A CN103004696A (en) | 2011-09-23 | 2011-09-23 | Method for inducing Dysmicoccus neobrevipes Beardsley infertility |
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| CN2011102849781A CN103004696A (en) | 2011-09-23 | 2011-09-23 | Method for inducing Dysmicoccus neobrevipes Beardsley infertility |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115088681A (en) * | 2022-08-02 | 2022-09-23 | 浙江省农业科学院 | Method for obtaining sterile male worms of tomato leaf miner and application of sterile male worms in prevention and control of tomato leaf miner |
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| CN102084850A (en) * | 2010-12-05 | 2011-06-08 | 天津出入境检验检疫局动植物与食品检测中心 | Method for breeding dysmicoccus neobrevipes |
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Patent Citations (6)
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| US4212267A (en) * | 1978-12-08 | 1980-07-15 | Patterson Irvin G | Insect study station |
| KR100426462B1 (en) * | 2001-02-16 | 2004-04-17 | 대한민국 | Mass rearing methode and device of Orius spp |
| WO2009034178A1 (en) * | 2007-09-14 | 2009-03-19 | Faculté Universitaire des Sciences Agronomiques de Gembloux | Treatment method using biological control |
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| CN102077822A (en) * | 2010-12-17 | 2011-06-01 | 中华人民共和国天津出入境检验检疫局 | Method for circular emission reduction for fumigant |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115088681A (en) * | 2022-08-02 | 2022-09-23 | 浙江省农业科学院 | Method for obtaining sterile male worms of tomato leaf miner and application of sterile male worms in prevention and control of tomato leaf miner |
| CN115088681B (en) * | 2022-08-02 | 2024-03-08 | 浙江省农业科学院 | Method for obtaining sterile male worms of tomato leaf miner and application of sterile male worms in pest control |
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Application publication date: 20130403 |