CN101185434A - Method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn - Google Patents
Method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn Download PDFInfo
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- CN101185434A CN101185434A CNA2007101508872A CN200710150887A CN101185434A CN 101185434 A CN101185434 A CN 101185434A CN A2007101508872 A CNA2007101508872 A CN A2007101508872A CN 200710150887 A CN200710150887 A CN 200710150887A CN 101185434 A CN101185434 A CN 101185434A
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- wheat
- methyl bromide
- stifling
- tck
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Abstract
The invention relates to a treatment method of a Tilletia controversa Kuhn (TCK) of wheat, in particular to a method to kill the TCK of the wheat through a methyl bromide fumigant. The method comprises that: the wheat with the TCK is enclosed into a closed space, and the methyl bromide with a weight ratio concentration of 98 percent is put into the closed space holding the wheat to fumigate the wheat. The fumigation is carried out in a preset dosage (180g/m<3>, 90g/m<3>) and at the ambient temperature (20 DEG C) and under the relative humidity of 60 to 70 percent; the fumigating time is 120h in the dosage of 180g/m<3> for one time, and 2 multiplied by 120h in the dosage of 90g/m<3> for two times. The method can kill the TCK of the wheat by 100 percent, and the operation is safe, reliable and can be widely popularized and applied.
Description
Technical field
The present invention relates to dwarf bunt of wheat bacterium (Tilletia controversa K ü hn, the method for TCK) handling, particularly a kind of method of applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn.
Background technology
TCK be in the wheat class disease harm maximum, be difficult to a kind of dangerous fungal disease preventing and treating and thoroughly eradicate.The germ systemic infection is downgraded wheat class plant, and the seed on the sick fringe is replaced by black powder.The general popular time can make wheat yield 30%～50%, and during serious harm, the underproduction can be up to more than 75%.Domestic someone once carried out investigation, stored the TCK epidemic disease wheat for 5 years in the silo, and its germ is biologically active still.TCK germ teleutospore and mycoceicidum can be with the wheat long-distance communications.Present about 29 countries of this disease five continents all over the world, the part producing region of some main wheat exported countrys of its Middle and North America (as 7 states of US West) and Europe is TCK epidemic-stricken areas.China classifies the TCK germ as always and forbids the quarantine harmful organisms that enters the territory.
The processing of TCK epidemic disease wheat mainly contains heating power facture, epoxy ethane fumigation, irradiation sterilization method, and the heating power facture is owing to handle back wheat quality decline, and disposal ability is limited and speed is slower, thereby is not used; Radiation treatment is because the electron beam irradiation device flexibility is relatively poor and the electron beam penetration capacity is more weak and not seen widespread use.The epoxyethane fumigation technology is comparatively ripe, but because oxirane is inflammable, explosive, practical application is affected, abroad the bactericidal effect of TCK in wheat seed and the soil once there was report in recent years with regard to Celfume, kill wheat TCK germ but whether this bactericidal effect can be suitable for, reach a conclusion after awaiting to test, analyzing.
Summary of the invention
The objective of the invention is: because diffusion, permeance property, processing safety and the insecticidal effect of Celfume is that other fumigant is incomparable, and has broad-spectrum bactericidal action, can the kill bacteria gemma, fungi and germ etc.Applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn, at first to study the influence of the parameters such as dosage, wheat environmental temperature and processing time that apply the methyl bromide fumigation agent to wheat TCK killing effect, find out 100% critical concentration of killing the Celfume of TCK, under the condition of constant temperature sealing, inorganic bromine content will reach safety and sanitation standard in the wheat after the methyl bromide fumigation agent is stifling simultaneously.
For achieving the above object, the invention provides a kind of method of applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn.
This method comprises: will be with the wheat of dwarf bunt bacterium the confined space of packing into, and be that 98% methyl bromide fumigation agent is rendered in the confined space that loads wheat and fumigated with weight ratio concentration.
This method comprises the once stifling processing of employing or twice stifling processing, adopts once stifling processing:
(1) dosage of wheat confined space: 180g/m is rendered in the methyl bromide fumigation agent
3
(2), environmental temperature: 20 ℃;
(3), relative moisture: 60～70%;
(4), fumigation time: 120h.
Adopt the stifling processing of secondary, stifling for the first time the processing:
(1) dosage of wheat confined space: 90g/m is rendered in the methyl bromide fumigation agent
3
(2), environmental temperature: 20 ℃;
(3), relative moisture: 60～70%;
(4), fumigation time: 120h;
Stifling for the second time processing the: repeat stifling for the second time the processing again according to for the first time stifling processing.
The beneficial effect that the present invention produces is: can 100% kill wheat TCK germ, handling safety, reliable, extensively popularization and application.
The present invention is in order to find out 100% conditional parameter of killing wheat TCK germ, once in laboratory, carried out preliminary experiment, under laboratory's condition, the TCK germ that will detect from the winter wheat of U.S.'s import is placed in the homemade stifling bucket stifling, take different Celfume dosage, temperature and processing time, draw different test datas at last, see (table 1).
| Treatment temperature ℃ | Processing time h | Dosage g/m 3 | Handle | Concentration actual measurement mean value g/m 3 | Light raw meat germination rate % | Short raw meat germination rate % | Short raw meat relative death rate % |
| 25 | 72 | 130 140 150 | 1 2 1 2 1 2 | 124.1 127.1 130.8 130.9 139.2 132.5 | 0 0.02 0 0 0 0 | 0.015 0.02 0.01 0.01 0.01 0 | 99.97 99.96 99.98 99.98 99.98 100 |
| 15 | 120 | 120 130 140 | 1 2 1 2 1 2 | 115.4 112.6 115.3 121.4 131.4 127.2 | 0.56 0.23 0.01 0 0 0 | 1.18 0.95 0.01 0.20 0 0.01 | 97.6 98.1 99.98 99.6 100 99.98 |
| 25 | 120 | 100 110 120 | 1 2 1 2 1 2 | 101.1 105.4 114.1 118.6 119.8 115.2 | 0 0 0 0 0 0 | 0.015 0 0.01 0 0 0 | 99.98 100 99.8 100 100 100 |
| 25 | 168 | 90 100 110 | 1 2 1 2 1 2 | 84.9 85.1 91.0 84.0 93.1 96.0 | 0.01 0 0 0 0 0 | 2.50 1.20 0.80 0.05 0.01 0 | 95.59 97.88 98.59 99.91 99.98 100 |
(table 1)
When temperature is 25 ℃ as can be seen from (table 1), have only, or 100% effect of killing TCK just appears in twice processing in 2 * 120 hours simultaneously with the methyl bromide fumigation of 1,20g,/m3 120 hours.That is to say, under 25 ℃ of conditions,, can 100% kill TCK with the methyl bromide fumigation of 1,20g,/m3 120 hours.Other conditions all cannot 100% be killed TCK.
Result of the test shows that Celfume has killing action to the TCK germ, and its effect raises with temperature and strengthens.By prolonging the processing time of Celfume, reduce the using dosage of Celfume, to reduce influence to wheat quality.
But temperature should be set at about 20 ℃ of constant temperature in reality is fumigated the epidemic disease wheat, because wheat can adsorb a part of Celfume in stifling, reduces the bactericidal effect of Celfume, and lower temperature also can reduce the bactericidal effect of Celfume simultaneously.Therefore wanting to reach 100% effect of killing TCK must improve the concentration of Celfume or prolong fumigation time.The parameter that draws with reference to preliminary experiment under the constant situation of fumigation time (120h), brings up to 180 with dosage
3, reached 100% sterilization effects, simultaneously can also be with dosage 180g/m
3Being divided into twice fumigates.
Embodiment
Celfume (the weight ratio concentration 98%) fumigant (gas) that this method adopts is produced by the Dead Sea, Lianyun Harbour Bromine Compounds Ltd..Adopt the sterilization effect of methyl bromide fumigation agent to wheat TCK in order to verify under above-mentioned stifling parameter condition, the present invention carries out following test to the wheat that has the TCK germ and handles:
Epidemic disease wheat (useful load 50%) is placed in the stifling bucket of stainless steel (self-control) that has seal cover, methyl bromide fumigation agent gas cylinder carrier pipe pin is inserted in the stifling bucket, according to the dosage (180g/m that sets
3, 90g/m
3) throw in, and under constant temperature (20 ℃), relative moisture (60～70%) condition, carry out:
180g/m
3Dosage, 120h are once stifling;
90g/m
3Dosage, 2 * 120h secondary is stifling.
Celfume is seen (table 2) to the sterilization effect of TCK under the 50% useful load condition.
| 20 ℃ of temperature T emperature | Processing time h Exposure time | Dosage g m -3 Dosage | Concentration actual measurement mean value gm -3 Average conentration | Light raw meat germination rate % Teliospores germination of T.foetida | TCK germination rate % Teliospores germination of T.controversa | TCK relative death rate % Relative mortality of TCK | ||
| Processed group Treated | Control group Control | Processed group Treated | Control group Control | |||||
| 120 2×120 | 180 90 | 139.95 92.05 | 0.035 0.000 | 26.50 26.50 | 0.000 0.000 | 12.40 12.40 | 100.00 100.00 | |
(table 2)
Use the methyl bromide fumigation agent and can 100% kill wheat TCK sterilization, its dosage, temperature relative moisture and processing time index choice all have in various degree influence, particularly dosage, temperature and processing time to sterilization effect.
Take this method can realize that the wheat that the scene is had the TCK germ handles:
The tote (as steamer, silo etc.) that at first will be loaded with the TCK wheat is airtight good with encapsulant, the carrier pipe pin that with weight ratio concentration is 98% methyl bromide fumigation agent (gas) gas cylinder inserts in the confined space that loads wheat, according to the dosage of setting the methyl bromide fumigation agent is rendered in the confined space, can be taked once stifling the processing or the stifling processing of secondary.
Adopt once stifling the processing:
(1) dosage of wheat confined space: 180g/m is rendered in the methyl bromide fumigation agent
3
(2) environmental temperature: 20 ℃;
(3) relative moisture: 60～70%;
(4) fumigation time: 120h.
Adopt the stifling processing of secondary, stifling for the first time the processing:
(1) dosage of wheat confined space: 90g/m is rendered in the methyl bromide fumigation agent
3
(2) environmental temperature: 20 ℃;
(3) relative moisture: 60～70%;
(4) fumigation time: 120h;
Stifling for the second time processing the: repeat stifling for the second time the processing again according to for the first time stifling processing.Just with single treatment dosage 180g/m
3Be divided into each 90g/m that is
3Dosage carries out twice and fumigates.
Because the residual main form with inorganic bromide ion of Celfume exists on the wheat after treatment, the usage amount of inorganic bromine content and methyl bromide fumigation agent is linear in the wheat.Therefore will carry out bromine content to the wheat after stifling the processing and detect, if bromine content is higher, can take to be mixed with the untreated state yield wheat ratio in 1: 2, the total bromine content of the wheat after being mixed significantly descends, and all can be reduced under the safety and sanitation standard.
Claims (3)
1. the method for an applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn, it is characterized in that, this method comprises: will be with the wheat of dwarf bunt bacterium the confined space of packing into, and be that 98% methyl bromide fumigation agent is rendered in the confined space that loads wheat and fumigated with weight ratio concentration.
2. the method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn as claimed in claim 1 is characterized in that, adopts once stifling the processing:
(1), the dosage of wheat confined space: 180g/m is rendered in the methyl bromide fumigation agent
3
(2), environmental temperature: 20 ℃;
(3), relative moisture: 60～70%;
(4), fumigation time: 120h.
3. the method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn as claimed in claim 1 is characterized in that: adopt that secondary is stifling to be handled, stifling for the first time the processing:
(1), the dosage of wheat confined space: 90g/m is rendered in the methyl bromide fumigation agent
3
(2), environmental temperature: 20 ℃;
(3), relative moisture: 60～70%;
(4), fumigation time: 120h;
Stifling for the second time processing the: repeat stifling for the second time the processing again according to for the first time stifling processing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101508872A CN101185434A (en) | 2007-12-11 | 2007-12-11 | Method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101508872A CN101185434A (en) | 2007-12-11 | 2007-12-11 | Method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn |
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| Publication Number | Publication Date |
|---|---|
| CN101185434A true CN101185434A (en) | 2008-05-28 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101508872A Pending CN101185434A (en) | 2007-12-11 | 2007-12-11 | Method for applying methyl bromide fumigation agent to kill Tilletia controversa Kuehn |
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| CN (1) | CN101185434A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004696A (en) * | 2011-09-23 | 2013-04-03 | 天津出入境检验检疫局动植物与食品检测中心 | Method for inducing Dysmicoccus neobrevipes Beardsley infertility |
| CN113367110A (en) * | 2021-07-27 | 2021-09-10 | 海口海关热带植物隔离检疫中心 | Steaming heat insecticidal quarantine treatment method for mangoes |
-
2007
- 2007-12-11 CN CNA2007101508872A patent/CN101185434A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004696A (en) * | 2011-09-23 | 2013-04-03 | 天津出入境检验检疫局动植物与食品检测中心 | Method for inducing Dysmicoccus neobrevipes Beardsley infertility |
| CN113367110A (en) * | 2021-07-27 | 2021-09-10 | 海口海关热带植物隔离检疫中心 | Steaming heat insecticidal quarantine treatment method for mangoes |
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Open date: 20080528 |