CN115088681A - Method for obtaining sterile male worms of tomato leaf miner and application of sterile male worms in prevention and control of tomato leaf miner - Google Patents
Method for obtaining sterile male worms of tomato leaf miner and application of sterile male worms in prevention and control of tomato leaf miner Download PDFInfo
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Abstract
The invention provides a method for obtaining sterile male worms of tomato leaf miner and application of the sterile male worms in pest control, and relates to the technical field of agricultural pest control. The method for obtaining sterile male worms of tomato leaf miner provided by the invention adopts 137 The male pupa of the tomato leaf miner is subjected to Cs-gamma ray irradiation treatment to obtain the sterile male adult of the tomato leaf miner, wherein the irradiation period is 2-3d of the male pupa before eclosion, the irradiation dose is 200-plus-300 Gy, and the irradiation dose rate is 0.8-1.2 Gy/min. According to the method for preventing and controlling the tomato leaf miner, after the irradiation-treated tomato leaf miner male sterile adults are continuously and massively released in the field, the tomato leaf miner male sterile adults compete with wild male adults for mate, so that the survival rate of offspring is greatly reduced, and the population number of the tomato leaf miner is effectively reducedAmount of the compound (A).
Description
Technical Field
The invention relates to the technical field of agricultural pest control, in particular to a method for obtaining sterile male insects of tomato leaf miner and application of the sterile male insects in insect control.
Background
The tomato leaf miner Tuta absoluta (Meyrinck) belongs to Lepidotea Lepidotera Meyensis Gelechiideae, is an important pest on tomatoes, can damage nearly 40 plants including potatoes, eggplants and sweet peppers, is mainly damaged by larvae, can submerge leaf flesh to form a submerged channel and eat young fruits, top tips, axillary buds and tender stems, causes a great amount of yield reduction, and is called as Ebola virus on the tomatoes if the yield loss of the improper tomatoes is up to 100 percent. The insect is sourced from peru in south america and then rapidly spread to other latin american countries, and since first discovered in spain in 2006, short and short 3-4 years have spread throughout the countries in the mediterranean region, and has now successfully invaded south america, north america, europe, africa, asia including over 100 countries, and is an important devastating pest on tomato worldwide. The insect is discovered for the first time in 2017 in Xinjiang in China, the insect is damaged in Yunnan in the next year, and then invades a lot of provinces in succession, and because the tomato leaf miner has extremely high spreading invasion speed and extremely high harm capability, the insect is likely to cause great outbreaks in China in a short time, and seriously threatens the tomato planting industry, so that effective green prevention and control measures are urgently needed to be developed, and the healthy development of the tomato planting industry in China is guaranteed.
At present, the control method for tomato leaf miner mainly comprises the traditional chemical agent control method and the sex pheromone cluster trapping and killing method. The chemical agent prevention and control method mainly targets the larvae, the hidden leaves and hidden fruits of the tomato leaf miner larvae are harmful, so that the larvae are difficult to be killed by the chemical agent, and the insects have drug resistance to various agents, so that the prevention and control effect is not ideal; although the sex pheromone cluster trapping method targets the imago, the sex pheromone cluster trapping method can only trap and kill male imago, and the imago has the characteristic of multiple mating, so that the pest control effect is poor.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The first purpose of the present invention is to provide a method for obtaining sterile male worms of tomato leaf miner, so as to solve at least one of the above problems.
The second purpose of the invention is to provide the application of the method for obtaining the sterile male worms of tomato leaf miners in the prevention and control of the tomato leaf miners.
In a first aspect, the invention provides a method for obtaining sterile males of tomato leaf miner, which comprises the following steps:
by using 137 C-gamma ray irradiation treatment of male pupa of tomato leaf miner to obtain sterile male adult tomato leaf miner; the irradiation dose of the irradiation treatment is 200-300 Gy;
the irradiation dose rate of the irradiation treatment is 0.8-1.2 Gy/min.
As a further technical scheme, the male and female pupae are distinguished according to the characteristics that the reproductive orifices of the male and female tomato leaf miner worms and females are respectively arranged on the 8 th section and the 9 th section of the abdomen.
As a further technical scheme, the tomato leaf miner male pupa is 2-3d before eclosion.
As a further technical scheme, the method for obtaining the male pupae of the tomato leaf miner 2-3d before eclosion comprises the following steps: picking up male pupa of tomato leaf miner with golden body color and black eyespot.
As a further technical scheme, the irradiation dose of the irradiation treatment is 300 Gy; the irradiation dose rate of the irradiation treatment is 1 Gy/min.
In a second aspect, the invention provides the application of the method in the control of tomato leaf miner.
In a third aspect, the invention provides a method for controlling tomato leaf miner, which comprises the following steps: and releasing the sterile male adult tomato leaf miner in the field.
As a further technical scheme, the radiation-treated tomato leaf miner male sterile adult is released in a ratio of sterile male worms to field wild male worms of 15:1-25: 1.
As a further technical proposal, the medicine is released every 8 to 12 days for 4 to 6 times.
As a further technical scheme, the medicine is released once every 10 days and 5 times.
Compared with the prior art, the invention has the following beneficial effects:
the method for obtaining sterile male worms of tomato leaf miner provided by the invention adopts 137 The male pupa of the tomato leaf miner is irradiated by the Cs-gamma rays to obtain the sterile male adult of the tomato leaf miner, compared with a radiation source 60 Co is more stable in irradiation dose and safer in operation, and compared with the method of using X-rays for irradiation, the method has the advantages that the absorbed dose is more uniform and the cost is lower, wherein the irradiation dose of irradiation treatment is 200-300Gy, and the irradiation dose rate of irradiation treatment is 0.8-1.2 Gy/min. Foreign researches show that the tomato leaf miner male pupa is treated by X rays (the irradiation dose is 200-250Gy and the irradiation dose rate is 0.679Gy/s) to obtain the tomato leaf miner sterile male imagoes, but compared with the method reported in foreign countries, the method for obtaining the tomato leaf miner sterile male imagoes provided by the invention has the advantages that the obtained sterile male imagoes have higher eclosion rate, stronger mating competitiveness, lower offspring survival rate and the ratio of the survival imagoes is biased to the males.
The method for obtaining the sterile male adult tomato leaf miner provided by the invention is applied to the prevention and control of the tomato leaf miner, after the irradiated sterile male adult tomato leaf miner is continuously released in a large amount in a field, the sterile male adult tomato leaf miner competes with a wild male adult to mate, so that the survival rate of offspring is greatly reduced, and the population number of the tomato leaf miner is effectively reduced. The invention realizes the suppression of the tomato leaf miner population by controlling the mating of adults, thereby not only overcoming the dilemma that the control effect of chemical agents is not ideal due to the characteristics of larval leaf miner and fruit miner as pests, but also solving the problem that the pest control effect of the tomato leaf miner with the characteristic of multiple mating is poor due to the fact that only male adults are trapped and killed by a sex pheromone cluster trapping and killing method, and providing a new green and effective control method for the control of the tomato leaf miner. Meanwhile, compared with the traditional control technology, the invention has the advantages of environmental friendliness, strong specificity, no harm to non-target organisms, lasting control effect, no resistance and the like.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a graph showing the effect of gamma irradiation treatment at different doses on male insects of the F0 generation tomato leaf miner 2-3d before eclosion;
FIG. 2 is the effect of different doses of gamma irradiation treatment on male worms of the F0 generation tomato leaf miner at 4-5d before eclosion;
FIG. 3 is the effect of different doses of gamma irradiation treatment on the tomato leaf miner generation F1;
FIG. 4 is the effect of gamma-ray irradiation at 300Gy dose on the fecundity of tomato leaf miner F1 generation and the hatchability of eggs of F2 generation.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to embodiments and examples, but those skilled in the art will understand that the following embodiments and examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. Those who do not specify the conditions are performed according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In a first aspect, the invention provides a method for obtaining sterile males of tomato leaf miner, which comprises the following steps:
by using 137 And (3) carrying out Cs-gamma ray irradiation treatment on the male pupae of the tomato leaf miner to obtain the sterile male adult of the tomato leaf miner. The irradiation dose of the irradiation treatment may be, for example, but not limited to, 200Gy, 250Gy, or 300 Gy;
the irradiation dose rate of the irradiation treatment can be, but is not limited to, 0.8Gy/min, 0.9Gy/min, 1Gy/min, 1.1Gy/min or 1.2Gy/min, for example.
The sterile male adult tomato leaf miner obtained by the method can greatly reduce the survival rate of offspring without influencing the eclosion and mating competitiveness of the male adult tomato leaf miner, and can be applied to the control of the tomato leaf miner.
In some preferred embodiments, male and female pupae can be distinguished according to the characteristics of tomato leaf miner male and female reproductive apertures in abdominal sections 8 and 9, respectively.
In some preferred embodiments, the tomato leaf miner male pupa is a tomato leaf miner male pupa 2-3d before eclosion.
The method for obtaining the male pupae of the tomato leaf miner 2-3d before eclosion comprises the following steps: picking up male pupa of tomato leaf miner with golden body color and black eyespot.
When selecting the pupa irradiation period, two indexes of male pupa sterility effect and male pupa damage need to be considered comprehensively, because the period of irradiation treatment of pupa is too early, although the ideal male pupa sterility effect can be achieved, the damage to pupa itself is too large, and the sterility effect is not ideal due to too late irradiation period, the invention selects the pupa in middle period for experiment. The inventor researches and discovers that the effect of irradiation treatment on the tomato leaf miner male pupa 2-3d before eclosion is better, the eclosion and mating competitiveness of the male worms can be further influenced, and the survival rate of offspring can be further reduced, because if the irradiation period is earlier, the tomato leaf miner male pupa 4-5d before eclosion is treated, the pupa death rate and the deformity ratio are obviously increased, the service life is obviously shortened, and the male worms are greatly damaged.
In some preferred embodiments, the irradiation dose of the irradiation treatment is 300Gy, and the irradiation dose rate of the irradiation treatment is 1 Gy/min.
Through further optimization and adjustment of the irradiation dose and the irradiation dose rate of irradiation treatment, the survival rate of the offspring of the male worms is further greatly reduced while the eclosion and mating competitiveness of the male worms are not influenced.
In a second aspect, the invention provides an application of a method for obtaining sterile male worms of tomato leaf miners in the prevention and control of the worms.
The tomato leaf miner sterile male adult obtained by the method can greatly reduce the survival rate of offspring while not influencing the eclosion and mating competitiveness of male worms, and can be used for controlling the male adult.
In a third aspect, the invention provides a method for controlling tomato leaf miner, comprising the following steps: and releasing the sterile male adult tomato leaf miner in the field.
After the irradiation-treated tomato leaf miner male adult male sterile adults are continuously and massively released in the field, the tomato leaf miner male sterile adults compete with wild male adults for mate, so that the survival rate of offspring is greatly reduced, and the population quantity of the tomato leaf miner is effectively reduced.
In some preferred embodiments, the number of sterile male adults that release the radiation-treated tomato leaf miner is: releasing the irradiated male sterile adults of the tomato leaf miner according to the ratio of the male sterile adults to the wild field males of 15:1-25:1 according to the number of the male sterile adults of the tomato leaf miner in the field.
In some preferred embodiments, the release is by: the release is carried out once every 8-12 days for 4-6 times, preferably once every 10 days for 5 times, and the release mode can more efficiently realize the control of the tomato leaf miner.
The invention is further illustrated by the following specific examples and comparative examples, but it should be understood that these examples are for purposes of illustration only and are not to be construed as limiting the invention in any way.
Example 1 selection of tomato leaf miner male pupa irradiation period
The population of the tomato leaf miner is collected from a tomato field (east longitude 102 degrees 57 '78', north latitude 24 degrees 34 '16') of Yuxi city, Yunnan province in 2019 in 6 months. After the collected population is brought back to a laboratory, tomato plants are taken as host plants, and are raised in an artificial climate box (the temperature is 25 +/-1 ℃, the relative humidity is 60% +/-5%, the photoperiod is 16L:8D) for continuous generation, so as to establish a stable population. Selecting a large number of pupae, distinguishing female pupae according to the characteristics of female and male tomato leaf miner on the 8 th and 9 th abdominal sections respectively, selecting male pupae with dark red eye spots and yellow-green body color as male pupae before eclosion 4-5d, and selecting male pupae with black eye spots and yellow-yellow body color as male pupae before eclosion 2-3 d. Gently picking male pupa of tomato leaf miner with writing brush and placing in flat-bottomed glass tubes (diameter 1.5cm, length 8cm), placing 5 pupas in each glass tube, and using 137 The irradiation treatment is carried out by Cs-gamma rays, wherein the dosage rate is 1Gy/min, the irradiation dose is set to be 100, 200, 300 and 400Gy, and the male worms without the irradiation treatment are used as control. Each dose of 5 male pupae was 1 replicate, for a total of 10 replicates. Observing the emergence condition of the male pupae of the tomato leaf miner of the irradiation treatment group control group, and counting the emergence rate (emergence number/pupae number), the malformation ratio (malformation number/emergence number) and the service life of the male adults after emergence.
As shown in fig. 1 and fig. 2 (different lower case letters indicate that the difference is significant (P <0.05)) through Duncan's new repolarization method, when 2-3d tomato leaf miner male pupae before eclosion is selected for irradiation, compared with a control group, gamma-ray irradiation does not affect the eclosion rate of F0 generation, while the malformation rate is increased and the service life is reduced with the increase of irradiation dose, but the male worm is greatly injured only when the irradiation dose reaches 400 Gy; when the male pupae 4-5d before eclosion are selected for irradiation, compared with a control group, with the increase of irradiation dose, the eclosion rate and the service life are obviously reduced, the malformation ratio is obviously increased, and huge damage is caused to the male insects.
Therefore, the method selects the optimal period of the radiation period of the male pupa of the tomato leaf miner 2-3d before eclosion.
Example 2 preliminary screening of radiation dose of Male pupae of tomato leaf miner
The male pupae before 2-3d of emergence are subjected to gamma-ray irradiation treatment with the dosage of 100, 200, 300 and 400Gy, the male pupae of the F0 generation which is normally subjected to emergence are respectively paired with the single heads of the female imagoes which are not treated, the paired male and female imagoes which are not subjected to irradiation treatment are used as a control, the paired male and female imagoes are placed in a flat-bottomed glass tube (the diameter is 3cm, the length is 20cm) with tomato leaves for mating for 10d, and 10 pairs are respectively matched for each group of treatment. During the period, the leaves were changed every day and 20 eggs were gently picked up on fresh leaves with a brush pen under a microscope and placed in a petri dish to observe the hatching. When eggs hatch into larvae, fresh tomato leaves are added periodically for feeding by the larvae until they pupate and emerge as adults. During the period, F1 generation egg hatching rate, larva survival rate, the ratio of egg development to imago (number of imago/egg) and the ratio of male and female imago (number of female imago/number of male imago) are counted. The results are shown in fig. 3 (different lower case letters indicate that the difference is significant (P <0.05)) through the Duncan's new double-polarization method test, the gamma-ray irradiation is not favorable for the survival of the F1 generations, the hatchability of the eggs of the F1 generations after 100, 200, 300 and 400Gy gamma-ray irradiation treatment is respectively 70.28%, 47.49%, 31.98% and 16.60% of the control group, the larva survival rate is respectively 82.99%, 62.71%, 58.27% and 31.92% of the control group, the proportion of eggs developing into adults is respectively 58.90%, 29.86%, 14.11% and 3.27% of the control group, and the ratio of the survival adults is biased to male adults with the increase of the irradiation dose, and no female adult survives when the dose reaches 400 Gy. Therefore, the hatchability of the F1 generation egg, the pupation rate of the larva, the ratio of the egg to the adult (female adult/male adult) and the ratio of the egg to the adult are all obviously reduced along with the increase of the irradiation dose.
According to the influence of gamma-ray irradiation treatment with different doses on various biological parameter indexes of generations F0 and F1 of tomato leaf miner, the invention discovers that 300Gy irradiation treatment can greatly reduce the survival rate of generation F1 without influencing the eclosion of generation F0 male worms, so that the 300Gy is primarily screened as the optimal dose of the radiation sterility.
EXAMPLE 3 verification and determination of optimal dose for radiation infertility
1) Influence of mating competitiveness of F0 generation tomato leaf miner male imagoes
Setting 3 combinations of normal female adults, normal male adults and irradiation male adults in a ratio of 1:1:0, 1:0:1 and 1:1:1, respectively releasing 5 normal female adults, 5 normal male adults, 5 irradiation male adults, 5 normal female adults, 5 normal male adults and 5 irradiation male adults in a mating device according to the ratio, and recording the egg production and the egg hatching rate of each combination, wherein the irradiation male adults are the male adults emerged after the irradiation treatment of male pupae by gamma rays with the dosage of 300 Gy. Each group was treated for 6 replicates. And (4) calculating the competitive mating index (C) of the male adults subjected to the irradiation treatment. The specific calculation method is as follows:
wherein N is the number of normal male adults; s is the number of irradiated male adults; ha is the hatching rate of the normal mating of male and female adults; hs is the hatching rate of the mating eggs of the normal female adult and the irradiation male adult; ee is the hatching rate of the eggs of the mixed male adult and the normal female adult in a certain S/N ratio; e is an expected value of the egg hatching rate of the mating of the mixed male adult and the normal female adult in a certain S/N ratio.
The experimental result is shown in table 1, the oviposition amount and the egg hatchability of the male adults irradiated by the gamma ray with the dosage of 300Gy can be obviously reduced, and when the ratio of the normal female adults to the normal male adults to the irradiated male adults is 1:1:1, compared with the situation that only the normal female adults exist, the oviposition amount is reduced by 19.05%, and the egg hatchability is reduced by 31.21%; the mating competition value is 1.07, namely the irradiated male worms have the same mating competition power with the normal male worms. This indicates that 300Gy dose gamma-ray irradiation treatment does not affect the mating competitiveness of male adults, can compete with wild male adults for female adults, and can greatly reduce the population number when released in large quantities in the field.
Influence of irradiation treatment in dosage of 1300 Gy on mating competitiveness of male adult tomato leaf miner
Note: u represents normal adult males, and I represents adult males subjected to gamma-ray irradiation treatment with 300Gy dosage. The data in the table are mean ± sem. The letters in the same column indicate significant differences (P <0.05) as tested by Duncan's new complex range method.
2) Influence of growth and development of F1 generation tomato leaf miner
Irradiating the tomato leaf miner male pupae 2-3d before eclosion by using gamma rays with the dose of 300Gy, enabling the eclosion F0 generation male adults and normal female adults to mate and lay eggs, taking eggs generated by the mating of the normal female and male adults as a contrast, picking 200 eggs and placing only 1 egg on each tomato leaf blade for each group of treatment, observing the whole life cycle of the adult from egg development, and recording the F1 generation egg hatching rate, the larva survival rate, the pupal eclosion rate, the development period of each stage and the proportion of the female adults (the number of the female adults/the number of all the adults).
The experimental results are shown in table 2, the F1 generation egg hatchability, the larva survival rate, the pupa eclosion rate and the female adult proportion of the male insects treated by the gamma ray irradiation with 300Gy dose are all obviously reduced, and the 4 biological parameters are respectively only 36.14%, 65.83%, 71.90% and 33.33% of normal offspring; compared with normal offspring, the development period of eggs, larvae and pupae of the F1 generation after irradiation is obviously prolonged. Therefore, the population irradiated by the gamma ray with the dose of 300Gy is not easy to survive, the number of female adults is greatly reduced, and the population continuation is not facilitated.
Influence of gamma-ray irradiation of tomato leaf miner male insect on F1 generation at dose of 2300 Gy
Note: the data in the table are mean ± sem. Indicates significant differences at P <0.001 levels as measured by the t-test.
3) Influence of F1 generation tomato leaf miner fertility and F2 generation egg hatchability
The F1 generation female and male adults after 300Gy dose gamma ray irradiation treatment are respectively paired with uniheads of normal male and female adults without irradiation treatment, the normal male and female adults without irradiation treatment are used as a control, the paired adults are placed in a flat-bottom glass tube (the diameter is 3cm, the length is 20cm) provided with tomato leaves for mating until the adults die, and 15 pairs are respectively matched for each treatment. During the period, the leaves are changed every day, the egg laying amount of each group is counted under a microscope, 20 eggs are gently picked on fresh leaves by using a writing brush and placed in a culture dish, and the hatching rate of F2 generation eggs is counted.
The experimental results are shown in fig. 4 (wherein, in fig. 4, the egg production amounts of the male adult and the normal female adult (300M +0F) of the F1 generation after the irradiation treatment of the F1 generation and the normal female adult (300M +0F) of the 300Gy gamma ray and the female adult and the normal male adult (300F +0M) of the F1 generation after the irradiation treatment are mated, and the egg production amounts of the male adult and the normal female adult after the irradiation treatment of the F2 generation in fig. 4 are shown by different lower case letters to be obviously different (P <0.05) through the Duncan new double-pole difference method test, compared with a control group, the egg production amounts of the male adult and the normal female adult of the F1 generation after the irradiation treatment of the 300Gy dosage and the female adult and the F1 generation after the irradiation treatment are obviously reduced, namely 15.84% and 14.98% of the control group, and the F2 generation of eggs after the irradiation treatment is basically not hatched. The result shows that the sterility effect of the male insect of the tomato leaf miner caused by the 300Gy gamma ray irradiation can be continued to a plurality of generations, the inheritance is realized, and the population quantity of offspring can be greatly reduced.
According to the method, through the determination of the mating competitiveness of male adults of tomato leaf miner in F0 generation, the growth, development and propagation conditions of tomato leaf miner in F1 generation and the hatching rate of eggs in F2 generation, the fact that the male pupae of the tomato leaf miner is irradiated by the gamma rays with 300Gy dosage is found, the extremely low survival rate of offspring can be caused while the mating competitiveness of the male adults is not influenced, the population quantity is greatly reduced, and the 300Gy dosage is determined to be the optimal dosage of the radiation sterility indeed.
Example 4 field Release of sterile Male adults of tomato leaf miner
In a greenhouse where the tomato leaf miner is harmful, releasing the sterile male adult tomato leaf miner treated by irradiation according to the ratio of sterile male adult to field wild male adult of 15:1-25:1 in a greenhouse where the tomato leaf miner is harmful, and releasing for 4-6 times every 8-12 days. The released sterile male adults compete with wild male adults for mate, so that the survival rate of offspring is greatly reduced, the quantity of the tomato leaf miner population is effectively reduced, and the control effect of suppressing and even eradicating the tomato leaf miner population is achieved.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A method for obtaining sterile male worms of tomato leaf miners is characterized by comprising the following steps:
by using 137 C-gamma ray irradiation treatment of male pupa of tomato leaf miner to obtain sterile male adult tomato leaf miner; the irradiation dose of the irradiation treatment is 200-300 Gy; the irradiation dose rate of the irradiation treatment is 0.8-1.2 Gy/min.
2. The method of claim 1, wherein male and female pupae are differentiated according to the characteristics of tomato leaf miner male and female reproductive apertures in abdominal sections 8 and 9, respectively.
3. The method of claim 1, wherein the tomato leaf miner male pupa is a tomato leaf miner male pupa from 2-3d before eclosion.
4. The method of claim 3, wherein the pre-emergence 2-3d tomato leaf miner male pupae are obtained by a method comprising: picking up male pupa of tomato leaf miner with golden body color and black eyespot.
5. The method according to claim 1, characterized in that the irradiation treatment has an irradiation dose of 300 Gy; the irradiation dose rate of the irradiation treatment is 1 Gy/min.
6. Use of the method of any one of claims 1 to 5 for the control of tomato leaf miner.
7. A method for controlling tomato leaf miner is characterized by comprising the following steps: releasing sterile male adults of tomato leaf miner as claimed in any one of claims 1-5 in the field.
8. The method for controlling tomato leaf miner as claimed in claim 7, wherein the tomato leaf miner male sterile adults are released in a ratio of sterile male worms to field wild male worms of 15:1-25: 1.
9. The method for controlling tomato leaf miner as claimed in claim 7, wherein the release is performed once every 8-12 days and 4-6 times.
10. The method for controlling tomato leaf miner as claimed in claim 8, wherein the release is performed once every 10 days and 5 times.
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