CN102998786B - 用于显微镜的自动聚焦方法和设备 - Google Patents

用于显微镜的自动聚焦方法和设备 Download PDF

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Publication number
CN102998786B
CN102998786B CN201210346424.4A CN201210346424A CN102998786B CN 102998786 B CN102998786 B CN 102998786B CN 201210346424 A CN201210346424 A CN 201210346424A CN 102998786 B CN102998786 B CN 102998786B
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CN
China
Prior art keywords
microscope
mark
beam path
focus
spacing
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN201210346424.4A
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English (en)
Chinese (zh)
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CN102998786A (zh
Inventor
曼佛瑞德·库斯特
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Leica Microsystems Schweiz AG
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Leica Microsystems Schweiz AG
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Publication of CN102998786A publication Critical patent/CN102998786A/zh
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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G02B21/025—Objectives with variable magnification
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0012—Surgical microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/245—Devices for focusing using auxiliary sources, detectors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
    • G02B7/28—Systems for automatic generation of focusing signals
    • G02B7/282—Autofocusing of zoom lenses
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N7/00—Television systems
    • H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Surgery (AREA)
  • Microscoopes, Condenser (AREA)
  • Automatic Focus Adjustment (AREA)
CN201210346424.4A 2011-09-15 2012-09-14 用于显微镜的自动聚焦方法和设备 Expired - Fee Related CN102998786B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011082756A DE102011082756A1 (de) 2011-09-15 2011-09-15 Autofokussierverfahren und -einrichtung für ein Mikroskop
DE102011082756.0 2011-09-15

Publications (2)

Publication Number Publication Date
CN102998786A CN102998786A (zh) 2013-03-27
CN102998786B true CN102998786B (zh) 2017-11-03

Family

ID=47751009

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201210346424.4A Expired - Fee Related CN102998786B (zh) 2011-09-15 2012-09-14 用于显微镜的自动聚焦方法和设备

Country Status (4)

Country Link
US (1) US9036021B2 (enExample)
JP (1) JP6139834B2 (enExample)
CN (1) CN102998786B (enExample)
DE (1) DE102011082756A1 (enExample)

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US8958147B2 (en) * 2013-06-14 2015-02-17 Computer Power Supply, Inc. Apparatus for aiding manual, mechanical alignment of optical equipment
WO2015133176A1 (ja) * 2014-03-05 2015-09-11 株式会社 日立ハイテクノロジーズ 顕微分光装置
JP3220165U (ja) * 2015-09-24 2019-02-21 シナプティヴ メディカル (バルバドス) インコーポレイテッドSynaptive Medical (Barbados) Inc. 電動全視野適応型顕微鏡
JP2018537709A (ja) 2015-11-11 2018-12-20 スコピオ ラボズ リミテッド 異なる照明条件下で画像を生成するためのコンピュータ顕微鏡及び方法
DE102016108226A1 (de) * 2016-05-03 2017-11-09 Carl Zeiss Microscopy Gmbh Mikroskop
KR20170137364A (ko) * 2016-06-03 2017-12-13 삼성전자주식회사 전자기파 집속장치 및 이를 포함하는 광학장치
JP6800698B2 (ja) * 2016-10-21 2020-12-16 株式会社キーエンス 拡大観察装置および拡大観察装置の制御方法
US11160451B2 (en) 2016-11-22 2021-11-02 Sony Olympus Medical Solutions Inc. Apparatus, method, program, and system
GB201704770D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Predictive focus tracking apparatus and methods
DE102017101188B4 (de) * 2017-01-23 2021-09-30 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zum Mikroskopieren einer Probe
NL2018857B1 (en) 2017-05-05 2018-11-09 Illumina Inc Systems and methods for improved focus tracking using a light source configuration
NL2018853B1 (en) 2017-05-05 2018-11-14 Illumina Inc Systems and methods for improved focus tracking using a hybrid mode light source
NL2018854B1 (en) 2017-05-05 2018-11-14 Illumina Inc Systems and methodes for improved focus tracking using blocking structures
EP3514594B1 (en) * 2018-01-19 2025-01-08 Leica Instruments (Singapore) Pte. Ltd. Method for automatedly aligning a stand for a microscope, stand for a microscope and microscope assembly
DE102018119343B4 (de) * 2018-08-08 2022-11-03 Carl Zeiss Meditec Ag Verfahren zur Kalibrierung von Objekten in einem Referenzkoordinatensystem und Verfahren zum Tracking von Objekten
EP3850329A4 (en) * 2018-09-10 2022-06-22 Fluidigm Canada Inc. APPARATUS AND METHOD FOR AUTO-FOCUSING SAMPLE IMAGING
DE102019113975B4 (de) 2019-05-24 2023-10-19 Abberior Instruments Gmbh Verfahren und Vorrichtung zum Überwachen des Fokuszustands eines Mikroskops sowie Mikroskop
CN114585958B (zh) * 2019-10-19 2024-05-28 美国赛库莱特生物有限公司 显微镜和用于使流体通道中的荧光标记成像的方法
KR20220118301A (ko) * 2019-12-31 2022-08-25 일루미나, 인코포레이티드 광학 샘플 분석에서의 오토포커스 기능
JP7625904B2 (ja) * 2021-03-09 2025-02-04 横河電機株式会社 オートフォーカス装置及び顕微鏡装置
JP7036396B1 (ja) * 2021-12-30 2022-03-15 株式会社 Zido オートフォーカス装置
CN119535760A (zh) * 2024-12-07 2025-02-28 华中科技大学 一种多焦点、多波段通道的自动聚焦共聚焦显微成像系统

Citations (1)

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CN1238840A (zh) * 1996-11-22 1999-12-15 莱卡显微镜系统股份公司 距离测量方法和距离测量仪

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DE3328821C2 (de) 1983-08-10 1986-10-02 Fa. Carl Zeiss, 7920 Heidenheim Autofokus für Mikroskope
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JPS6374272A (ja) * 1986-09-17 1988-04-04 Victor Co Of Japan Ltd オ−トフオ−カス装置
WO1992006359A1 (en) * 1990-10-09 1992-04-16 Metronics, Inc. Laser autofocus apparatus and method
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JPH05346533A (ja) * 1992-06-12 1993-12-27 Sony Corp 焦点検出方法
JP3537155B2 (ja) * 1993-05-07 2004-06-14 オリンパス株式会社 手術用顕微鏡
CH692254A5 (de) 1996-06-29 2002-04-15 Zeiss Carl Mikroskop mit einer Autofokus-Anordnung.
JP2000075213A (ja) * 1998-08-27 2000-03-14 Olympus Optical Co Ltd 手術用顕微鏡
DE10106698A1 (de) 2001-02-14 2002-08-29 Leica Microsystems Verfahren und Vorrichtung zum automatischen Fokussieren eines optischen Gerätes
DE10113084A1 (de) 2001-03-17 2002-09-19 Leica Microsystems Autofokus-Mikroskopsystem
JP2003241079A (ja) * 2002-02-22 2003-08-27 Mitsutoyo Corp 画像観察装置
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US7301133B2 (en) * 2005-01-21 2007-11-27 Photon Dynamics, Inc. Tracking auto focus system
DE102005024063B3 (de) * 2005-05-25 2006-07-06 Soft Imaging System Gmbh Verfahren und Vorrichtung zur optischen Abtastung einer Probe
DE102006040636B3 (de) 2006-05-15 2007-12-20 Leica Microsystems (Schweiz) Ag Autofokus-System und Verfahren zum Autofokussieren
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Also Published As

Publication number Publication date
US20130070076A1 (en) 2013-03-21
DE102011082756A1 (de) 2013-03-21
US9036021B2 (en) 2015-05-19
JP6139834B2 (ja) 2017-05-31
CN102998786A (zh) 2013-03-27
JP2013065015A (ja) 2013-04-11

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Granted publication date: 20171103