CN102993300A - 鸭坦布苏病毒多克隆抗体及其制备方法 - Google Patents
鸭坦布苏病毒多克隆抗体及其制备方法 Download PDFInfo
- Publication number
- CN102993300A CN102993300A CN2012104806696A CN201210480669A CN102993300A CN 102993300 A CN102993300 A CN 102993300A CN 2012104806696 A CN2012104806696 A CN 2012104806696A CN 201210480669 A CN201210480669 A CN 201210480669A CN 102993300 A CN102993300 A CN 102993300A
- Authority
- CN
- China
- Prior art keywords
- tembusu virus
- duck tembusu
- polyclonal antibody
- protein
- duck
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000686770 Duck Tembusu virus Species 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims description 15
- 210000002966 serum Anatomy 0.000 claims abstract description 24
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 13
- 239000000427 antigen Substances 0.000 claims abstract description 7
- 108091007433 antigens Proteins 0.000 claims abstract description 7
- 102000036639 antigens Human genes 0.000 claims abstract description 7
- 239000002671 adjuvant Substances 0.000 claims description 9
- 230000036039 immunity Effects 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- 239000000839 emulsion Substances 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 3
- 230000003053 immunization Effects 0.000 claims description 3
- 238000002649 immunization Methods 0.000 claims description 3
- 230000002163 immunogen Effects 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 13
- 230000004224 protection Effects 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 101710204837 Envelope small membrane protein Proteins 0.000 abstract description 8
- 101710145006 Lysis protein Proteins 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 4
- 241000710831 Flavivirus Species 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 241000272525 Anas platyrhynchos Species 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- 238000010254 subcutaneous injection Methods 0.000 description 7
- 239000007929 subcutaneous injection Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000000945 filler Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 241000272522 Anas Species 0.000 description 4
- 101150013191 E gene Proteins 0.000 description 4
- 229920002684 Sepharose Polymers 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000001524 infective effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000010255 intramuscular injection Methods 0.000 description 3
- 239000007927 intramuscular injection Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000010612 desalination reaction Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000012149 elution buffer Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000283707 Capra Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 1
- 101800001030 Non-structural protein 2A Proteins 0.000 description 1
- 101710144111 Non-structural protein 3 Proteins 0.000 description 1
- 101800001020 Non-structural protein 4A Proteins 0.000 description 1
- 101800001019 Non-structural protein 4B Proteins 0.000 description 1
- 101710144121 Non-structural protein 5 Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 240000007711 Peperomia pellucida Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 101710194807 Protective antigen Proteins 0.000 description 1
- 101000933967 Pseudomonas phage KPP25 Major capsid protein Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 102000018265 Virus Receptors Human genes 0.000 description 1
- 108010066342 Virus Receptors Proteins 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012913 medium supplement Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910001453 nickel ion Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210480669.6A CN102993300B (zh) | 2012-11-22 | 2012-11-22 | 鸭坦布苏病毒多克隆抗体及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201210480669.6A CN102993300B (zh) | 2012-11-22 | 2012-11-22 | 鸭坦布苏病毒多克隆抗体及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102993300A true CN102993300A (zh) | 2013-03-27 |
CN102993300B CN102993300B (zh) | 2014-07-16 |
Family
ID=47922463
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201210480669.6A Active CN102993300B (zh) | 2012-11-22 | 2012-11-22 | 鸭坦布苏病毒多克隆抗体及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102993300B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107202883A (zh) * | 2017-05-24 | 2017-09-26 | 徐成蔚 | 检测鸭坦布苏病毒的侧向流免疫层析测定产品及相关试剂与制备方法 |
CN108676078A (zh) * | 2018-05-23 | 2018-10-19 | 江苏省农业科学院 | 引起坦布苏病毒抗体依赖增强作用的抗原的应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102586193A (zh) * | 2012-02-10 | 2012-07-18 | 中国农业科学院上海兽医研究所 | 抗鸭坦布苏病毒的单克隆抗体、杂交瘤细胞株及其应用 |
-
2012
- 2012-11-22 CN CN201210480669.6A patent/CN102993300B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102586193A (zh) * | 2012-02-10 | 2012-07-18 | 中国农业科学院上海兽医研究所 | 抗鸭坦布苏病毒的单克隆抗体、杂交瘤细胞株及其应用 |
Non-Patent Citations (5)
Title |
---|
WANG C ET AL: "AFK09756.1", 《NCBI GENBANK》 * |
YAN,P ET AL: "AEL99350.1", 《NCBI GENBANK》 * |
姬希文等: "鸭坦布苏病毒E蛋白中和性单克隆抗体的制备", 《中国家禽》 * |
姬希文等: "鸭坦布苏病毒E蛋白的原核表达", 《中国动物传染病学报》 * |
杨少艳等: "鸭坦布苏病毒E基因的克隆表达及初步应用", 《浙江农业学报》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107202883A (zh) * | 2017-05-24 | 2017-09-26 | 徐成蔚 | 检测鸭坦布苏病毒的侧向流免疫层析测定产品及相关试剂与制备方法 |
CN107202883B (zh) * | 2017-05-24 | 2019-06-04 | 徐成蔚 | 检测鸭坦布苏病毒的侧向流免疫层析测定产品及相关试剂与制备方法 |
CN108676078A (zh) * | 2018-05-23 | 2018-10-19 | 江苏省农业科学院 | 引起坦布苏病毒抗体依赖增强作用的抗原的应用 |
Also Published As
Publication number | Publication date |
---|---|
CN102993300B (zh) | 2014-07-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101900731A (zh) | 一种区别检测猪瘟疫苗免疫与野毒感染抗体的elisa试剂盒及其制备方法 | |
CN107899008B (zh) | 一种猪流行性腹泻、猪传染性胃肠炎、猪丁型冠状病毒病三联亚单位疫苗 | |
CN109182380A (zh) | 杆状病毒表达的猪瘟e2亚单位疫苗的制备方法及应用 | |
CN101955545A (zh) | 一种多靶点重组基因及其蛋白在防治幽门螺旋杆菌感染中的应用 | |
CN102864157A (zh) | 一种副猪嗜血杆菌免疫保护性抗原 | |
CN102977194B (zh) | 一种鸭坦布苏病毒e蛋白基因及其应用 | |
CN108066755A (zh) | 一种抗羊包虫病感染的基因工程亚单位疫苗及其制备方法和应用 | |
CN102181457B (zh) | 密码子优化的艰难梭菌外毒素b氨基端基因序列及其核酸疫苗 | |
CN104974249A (zh) | 鸭疫里默氏菌OmpA/MotB截短重组蛋白、其抗体及制备方法和应用 | |
CN102993300B (zh) | 鸭坦布苏病毒多克隆抗体及其制备方法 | |
CN102167735A (zh) | 日本血吸虫SjTollip重组抗原蛋白及其制备方法和用途 | |
CN102443053A (zh) | 猪链球菌2型hp0245基因编码蛋白作为保护性抗原的应用 | |
CN101747416B (zh) | 拟态弧菌OmpU蛋白B细胞抗原多表位串联肽及其构建方法与用途 | |
CN104059134B (zh) | 败血性巴氏杆菌毒素重组蛋白及其应用 | |
CN101481675B (zh) | 一种抗猪瘟多表位dna疫苗及构建方法和应用 | |
CN102978219B (zh) | 一种弧菌交叉保护性抗原及其制备方法和应用 | |
CN105481953B (zh) | 作为猪繁殖与呼吸综合征病毒疫苗抗原的靶细胞特异性融合蛋白和疫苗组合物 | |
CN102240399B (zh) | 鳜传染性脾肾坏死病毒(isknv)orf093蛋白的应用 | |
CN104862331B (zh) | 一种可溶性表达马红球菌致病基因VapA蛋白的方法 | |
CN104610455A (zh) | 一种鸭坦布苏病毒基因工程亚单位疫苗 | |
CN108503696A (zh) | 一种酵母细胞表达的寨卡病毒亚单位疫苗 | |
CN105777909A (zh) | 一种猪趋化因子介导的a型口蹄疫靶向性复合表位蛋白及疫苗 | |
CN112279925B (zh) | 一种融合蛋白及一种犬弓形虫亚单位疫苗及其疫苗组合物 | |
CN103361317B (zh) | 一种猕猴IFN-γ的单克隆抗体杂交瘤细胞及制备方法和应用 | |
CN107653231A (zh) | 鸭源性冠状病毒弱毒株ibvdcv35及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB02 | Change of applicant information |
Address after: Chengyang Hongdao street Xuechi East Road 266114 Shandong city of Qingdao Province Applicant after: QINGDAO BOITE BIOPHARMACEUTICAL Co.,Ltd. Applicant after: QINGDAO VLAND BIOTECH Inc. Address before: Chengyang Hongdao street Xuechi East Road 266114 Shandong city of Qingdao Province Applicant before: QINGDAO BOITE BIOPHARMACEUTICAL Co.,Ltd. Applicant before: QINGDAO KDN PHARMACEUTICAL Co.,Ltd. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee |
Owner name: QINGDAO WEILAN BIOLOGICAL PRODUCT CO., LTD. Free format text: FORMER NAME: QINGDAO BOITE BIOPHARMACEUTICAL CO., LTD. |
|
CP01 | Change in the name or title of a patent holder |
Address after: Chengyang Hongdao street Xuechi East Road 266114 Shandong city of Qingdao Province Patentee after: QINGDAO VLAND BIOTECH GROUP CO.,LTD. Patentee after: QINGDAO VLAND BIOTECH Inc. Address before: Chengyang Hongdao street Xuechi East Road 266114 Shandong city of Qingdao Province Patentee before: QINGDAO BOITE BIOPHARMACEUTICAL Co.,Ltd. Patentee before: QINGDAO VLAND BIOTECH Inc. |
|
CP03 | Change of name, title or address |
Address after: 266000 Keyun Road 85, High tech Zone, Qingdao, Shandong Province Patentee after: Qingdao Blue Animal Health Group Co.,Ltd. Patentee after: QINGDAO VLAND BIOTECH Inc. Address before: 266114 Xuechi Road East, Hongdao Street, Chengyang District, Qingdao City, Shandong Province Patentee before: QINGDAO VLAND BIOTECH GROUP CO.,LTD. Patentee before: QINGDAO VLAND BIOTECH Inc. |
|
CP03 | Change of name, title or address |