CN102988492B - Cistanche cell disruption method and buccal tablet prepared from cistanche powder obtained by cistanche cell disruption method - Google Patents
Cistanche cell disruption method and buccal tablet prepared from cistanche powder obtained by cistanche cell disruption method Download PDFInfo
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- CN102988492B CN102988492B CN201210488774.4A CN201210488774A CN102988492B CN 102988492 B CN102988492 B CN 102988492B CN 201210488774 A CN201210488774 A CN 201210488774A CN 102988492 B CN102988492 B CN 102988492B
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Abstract
The invention relates to a cistanche cell disruption method. The cistanche cell disruption method provided by the invention comprises the following steps of: cleaning and slicing cistanche, and drying; carrying out coarse grinding at low temperature of minus 60 to 0 DEG C; placing coarse powder into a negative pressure machine, evacuating negative pressure and then instantly releasing negative pressure, and continuously repeating the process for 3-10 times; carrying out low temperature vacuum drying; and sterilizing. Cistanche powder after wall disruption is subjected to tabletting under the vacuum or nitrogen-filled environment to be prepared into buccal tablets, and oxidation is avoided, so that the buccal tablets are convenient to take and are more beneficial to absorption of a human body.
Description
Technical field
The invention belongs to medicinal plants applied research technical field, be specifically related to buccal tablet prepared by a kind of wall-breaking method of Herba Cistanches cell and the Herba Cistanches powder of the method acquisition.
Background technology
Herba Cistanches is the fleshy stem of the dry zone scale leaf of orobanchaceae plant cistanche (Cistanche deserticola Y.C.Ma), has kidney invigorating and YANG supporting, benefiting essence-blood, moisturizes the effects such as logical intestinal, slow down aging.Herba Cistanches is a kind of parasitic plant that colonizes in desert trees sacsaoul, red building root.Be distributed in the Inner Mongol, Ningxia, Gansu and Xinjiang, the good reputation that have " desert Radix Ginseng ", has high medical value, Cistanche deserticola Orobanchaceae endangered species, being the traditional rare Chinese medicine of China, is also one of tonification medicine that in successive dynasties kidney invigorating and YANG supporting class prescription, usage frequency is the highest.Its benefit property is gentle, long-term taking preferably.And contain multiple to the useful nutritional labeling of human body, trace element and vitamin etc.
The consumption of Herba Cistanches increases year by year, but its output can not meet the speed of domestic and international demand growth far away.Particularly prepare in Herba Cistanches medicine or health product process, often use more extensive processing extracting method, as heated and using liquid carrying to follow the example of, all can not realize abundant extraction.And leaching process can cause destruction in various degree to effective ingredient, thus the drug effect of greatly reducing.This is also the significant wastage to limited Herba Cistanches resource.
In order to save material, improve curative effect, there is in recent years a kind of Technology of plant cell wall breaking.Cell wall breaking is a kind of superfine powder process technology, and Chinese crude drug cell wall breaking micronizing process technology can fully discharge medical material effective ingredient.At present, both at home and abroad to most machinery broken wall law, breaking cellular wall by temperature difference method and the breaking wall by fermentation methods of adopting of Chinese crude drug.Because Herba Cistanches sugar content is higher, these method effects are all not ideal.Low and the long processing time of sporoderm-broken rate, sterilizing is difficult, and particularly process temperature is high; After breaking cellular wall, effective ingredient is free, is directly exposed in air, very easily by airborne dioxygen oxidation, effective ingredient is destroyed large.
Summary of the invention
For above-mentioned deficiency, the present invention relates to a kind of Herba Cistanches cell wall breaking method.Said method comprising the steps of:
Step 1, cleans Herba Cistanches after section, dry;
Step 2, carries out coarse powder under-60 ℃ of-0 ℃ of low temperature;
Step 3, puts into Negative pressure machine by coarse powder, abrupt release negative pressure after the negative pressure of finding time, 3-10 time continuously;
Step 4, low-temperature vacuum drying.
The gas being filled with when preferably, described step 3 discharges negative pressure is nitrogen.
Preferably, described step 4 is vacuum drying at the temperature of-40 ℃-0 ℃.
Preferably, described method also comprises step 5, is filled with nitrogen autoclaving.
Preferred, described step 5 is for being filled with nitrogen, autoclaving under the pressure of 300Mpa-750Mpa, and at abrupt release pressure.
Preferably, described step 2 is under negative pressure, to carry out coarse powder.
Preferred, described nitrogen is substituted by other noble gases.
Preferred, described nitrogen is substituted by other noble gases.
The invention still further relates to buccal tablet prepared by Herba Cistanches powder that a kind of Herba Cistanches cell wall breaking method obtains.
When preferably, described buccal tablet is packed, be filled with nitrogen.
Herba Cistanches powder prepared by the Herba Cistanches cell wall breaking method the present invention relates to, sporoderm-broken rate can reach more than 99%.And whole preparation process keeps cryogenic vacuum and carries out under the protection of nitrogen or noble gas, preserves to greatest extent the effective ingredient of Herba Cistanches, and the Herba Cistanches powder after breaking cellular wall does not need further ultra micro efflorescence always, the Herba Cistanches powder direct compression of formation.And the wall cell disruption powder of this Herba Cistanches is prepared as to buccal tablet at the environment lower sheeting of vacuum or inflated with nitrogen, and avoid oxidation, be convenient to take, be more conducive to absorption of human body.
The specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to description word.
Embodiment 1 takes 500g Herba Cistanches, cleans after section, dry; Carry out coarse powder, can use common Chinese medicine material crushing machine, higher for fear of local temperature in crushing process, under-40 ℃ of low temperature, carry out coarse powder; Coarse powder is put into Negative pressure machine, and negative pressure (vacuum-0.02--0.1Mpa) abrupt release negative pressure after 20 minutes, continuous 5 times, is conducive to being dried and cell wall being cracked and break of Herba Cistanches like this.
Embodiment 2 takes 500g Herba Cistanches, cleans after section, dry; Coarse powder under-10 ℃ of low temperature; Coarse powder is put into Negative pressure machine, negative pressure (vacuum-0.02--0.1Mpa) abrupt release negative pressure after 10 minutes, the gas being filled with while discharging negative pressure is nitrogen, continuous 3 times, those skilled in the art should be understood that, the nitrogen using in the present invention also can replace with noble gas, but considers preparation cost, preferred nitrogen; Vacuum drying at the temperature of-20 ℃, HIGH PRESSURE TREATMENT sterilizing in 30 minutes under 600Mpa, and abrupt release pressure, also can be used cobalt-60 radiation sterilization, but the condition of the wayward low temperature of cobalt-60 radiation sterilization or starvation, in order to reduce the oxidation of Herba Cistanches powder, preferably autoclaving is carried out sterilizing to Herba Cistanches powder, and Herba Cistanches is carried out after negative pressure abrupt release pressure, when autoclaving, adopt again high pressure abrupt release pressure, be more conducive to breaking cellular wall, make breaking cellular wall more complete.
Embodiment 3 takes 500g Herba Cistanches, cleans after section, dry;-40 ℃ and carry out coarse powder under negative pressure (vacuum-0.02--0.1Mpa); Coarse powder is put into Negative pressure machine, negative pressure (vacuum-0.02--0.1Mpa) abrupt release negative pressure after 10 minutes, continuous 3 times; HIGH PRESSURE TREATMENT sterilizing in 30 minutes abrupt release pressure under 300Mpa.
Embodiment 4 takes 500g Herba Cistanches, cleans after section, dry;-60 ℃ and carry out coarse powder under negative pressure (vacuum-0.02--0.1Mpa); Coarse powder is put into Negative pressure machine, negative pressure (vacuum-0.02--0.1Mpa) abrupt release negative pressure after 10 minutes, the gas being filled with while discharging negative pressure is nitrogen, continuous 3 times; Under 300Mpa, be filled with the sterilizing in 30 minutes of nitrogen HIGH PRESSURE TREATMENT, and abrupt release pressure.
The Herba Cistanches powder use HPLC(high performance liquid chromatography that embodiment 5 measures Herba Cistanches raw material and prepares by embodiment 1,2,3,4) measure the content of verbascoside in Herba Cistanches powder, getting the suspension of the Herba Cistanches powder of each embodiment 1,2,3,4 preparations laminates respectively, under 150 power microscopes, observe, record in each visual field not breaking wall of pollen cell number.The results are shown in following table 1:
Table 1
| The content of verbascoside | Sporoderm-broken rate (%) | |
| Herba Cistanches raw material | 100% | -- |
| Embodiment 1 | 90.2% | 92% |
| Embodiment 2 | 101.1% | 99% |
| Embodiment 3 | 97.7% | 99% |
| Embodiment 4 | 99.4% | 99% |
Embodiment 5, by the Herba Cistanches powder direct compression of embodiment 1,2,3,4 preparations, adopts negative pressure packaging machine to carry out negative pressure packing, and is filled with nitrogen, completes packing, product.
Herba Cistanches is unfavorable for superfine grinding because of its sugar content more, and the present invention carries out coarse powder-freezing-moment negative pressure-sterilizing-tabletting-negative pressure inflated with nitrogen packing-breaking cellular wall buccal tablet finished product repeatedly by Herba Cistanches.Feature according to Herba Cistanches buccal tablet of the present invention is: the buccal product that make with the wall cell disruption powder tabletting of Herba Cistanches, and its dose is few, and taking convenience has retained all substances of Herba Cistanches the inside.Active substance that like this can retained product is unlikely to destroy, and sterilizing raw material, by tablet machine tabletting, does not wherein add any adjuvant and additive, keeps exclusive local flavor and the characteristic of Herba Cistanches, prevents and other material generation chemical reaction.Again the raw material of sterilizing is packed and is filled with nitrogen by negative pressure packaging machine, complete packing, product.Because the Herba Cistanches powder after breaking cellular wall is easy to oxidized, so use negative pressure in packing, itself and oxygen are completely cut off, be filled with peculiar taste and nutrition that nitrogen can keep Herba Cistanches powder, avoid Herba Cistanches that the reactions such as oxidation occur, can make product preserve for a long time and not lose efficacy.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the general concept that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment describing.
Claims (8)
1. a Herba Cistanches cell wall breaking method, is characterized in that, described method mainly comprises the following steps:
Step 1, cleans Herba Cistanches after section, dry;
Step 2, carries out coarse powder under-60 ℃ of-0 ℃ of low temperature;
Step 3, puts into Negative pressure machine by coarse powder, abrupt release negative pressure after the negative pressure of finding time, 3-10 time continuously;
The gas being filled with while wherein, discharging negative pressure is nitrogen.
Step 4, vacuum drying at the temperature of-40 ℃-0 ℃.
2. Herba Cistanches cell wall breaking method as claimed in claim 1, is characterized in that, described method finally also comprises and is filled with the autoclaved step of nitrogen.
3. Herba Cistanches cell wall breaking method as claimed in claim 2, is characterized in that, autoclaving under the pressure of 300Mpa-750Mpa, and at abrupt release pressure.
4. Herba Cistanches cell wall breaking method as claimed in claim 1, is characterized in that, described step 2 is under negative pressure, to carry out coarse powder.
5. Herba Cistanches cell wall breaking method as claimed in claim 1, is characterized in that, described nitrogen is substituted by other noble gases.
6. Herba Cistanches cell wall breaking method as claimed in claim 2, is characterized in that, described nitrogen is substituted by other noble gases.
7. buccal tablet prepared by the Herba Cistanches powder that an employing obtains as the Herba Cistanches cell wall breaking method as described in arbitrary in claim 1-6.
8. buccal tablet prepared by the Herba Cistanches powder that Herba Cistanches cell wall breaking method as claimed in claim 7 obtains, is characterized in that, is filled with nitrogen during described buccal tablet packing.
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| CN103027957B (en) * | 2012-12-26 | 2014-09-24 | 孟令刚 | Cistanche cell disruption method and buccal tablet prepared by cistanche micropowder obtained by method |
| CN104738614B (en) * | 2013-12-31 | 2016-09-21 | 台建祥 | A kind of wolfberry health food and production technology thereof |
| CN104544098A (en) * | 2015-01-31 | 2015-04-29 | 张翔 | Cistanche essential tablet and preparation method thereof |
| CN105011159A (en) * | 2015-06-25 | 2015-11-04 | 孟令刚 | Herba cistanches powder making process and preparation and food prepared by utilizing the powder making process |
| CN108970777A (en) * | 2018-09-05 | 2018-12-11 | 湖南仟鹤康生物科技有限公司 | A kind of vacuum ultrasonic wall-breaking machine |
| CN111265569A (en) * | 2019-10-22 | 2020-06-12 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Preparation method of cistanche superfine powder |
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