CN102988195B

CN102988195B - Liposome anti-hair loss preparation

Info

Publication number: CN102988195B
Application number: CN201210524236.6A
Authority: CN
Inventors: 张年生; 祝贵财
Original assignee: BEIJING LAIMIRUIKE TECHNOLOGY DEVELOPMENT Co Ltd
Current assignee: BEIJING LAIMIRUIKE TECHNOLOGY DEVELOPMENT Co Ltd
Priority date: 2012-12-10
Filing date: 2012-12-10
Publication date: 2014-12-10
Anticipated expiration: 2032-12-10
Also published as: CN102988195A

Abstract

The invention relates to a liposome anti-hair loss cream, belonging to the technical field of cosmetics. The liposome anti-hair loss cream comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 0.003-3000.0 parts of phospholipid, 0.001-100.0 parts of vitamin H, 0.001-100.0 parts of ginkgetin, 0.001-10.0 parts of fibroin, 0.01-100.0 parts of collagen, 0.01-100.0 parts of histidine, and 0.001-200.0 parts of cholesterol. The liposome anti-hair loss cream provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1/200-1/300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The liposome anti-hair loss cream can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure hair health and increase the flexibility and elasticity.

Description

Liposome Anti-hair loss preparation

Technical field

The present invention relates to a kind of liposome anticreep hair cream, relate in particular to a kind of liposome anticreep hair cream that can increase hair elasticity, pliability, also can make anti-alopecia, belong to cosmetic product technical field.

Background technology

Skin is made up of epidermis, corium, subcutaneous fat and appendages.Epidermal area is outermost one deck of skin, is divided into from inside to outside: basal cell layer, prickle cell layer, granular cell layer, clear cell layer and horn cell layer.Skin appendages comprises hair, hair follicle, sebaceous gland and sweat gland.Skin mainly absorbs external substance by three approach, i.e. horny layer, pilosebaceous unit and the antiperspirant mouth of pipe.Its mesocuticle is the most important approach that skin absorbs, skin can only absorb some electrolyte under normal circumstances, small-molecule substance and liposoluble substance, can not directly absorb for some macro-molecular proteins, in daily life, most anticreep hair cream is aqueous solution, and epidermal growth factor, collagen protein, ginkgetin, the important life active compound such as histidine is all water miscible, and the exogenous lipid of horny layer secretion and the endogenous lipid of sebaceous gland Lipid Secretion are oily matter, and water soluble nutrients chance oiliness lipid is insoluble, as shown in Figure 1, therefore cause conventional water solublity anticreep hair cream utilization rate very little.

Summary of the invention

For overcoming the existing shortcoming of prior art, goal of the invention of the present invention is to provide a kind of liposome anticreep hair cream, and it,, taking liposome as carrying China, is carried to deep skin by various nutritional labelings, fully absorbed by human body, increase hair elasticity, pliability, also can make anti-alopecia.

For realizing described goal of the invention, a kind of liposome anticreep hair cream, it comprises the composition of following ratio of weight and number:

Preferably, also comprise the composition of following ratio of weight and number:

Compared with prior art, liposome anticreep hair cream provided by the invention, it,, taking liposome as carrying China, is carried to deep skin by various nutritional labelings, can increase hair elasticity, pliability, also can make anti-alopecia.

Brief description of the drawings

Fig. 1 is the schematic diagram of the skin absorption process of the skin care item that provide in prior art;

Fig. 2 is the schematic diagram of the skin absorption process of liposome anticreep hair cream provided by the invention.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is further illustrated.

Embodiment 1

This embodiment provides a kind of hair elasticity, pliability, and liposome anticreep hair cream that can anti-alopecia of increasing.Described liposome anticreep hair cream comprises the composition of the weight part ratio described in following table:

Composition	Concrete content mg
		Epithelical cell growth factor (EGF)	0.5-8.0
Human stem cell growth (SCGF)	0.01-0.1
		Phospholipid	0.003-3000.0
Biotin	0.001-100.0
		Ginkgetin	0.001-100.0
Fibroin	0.001-10.0
		Collagen protein	0.01-100.0
Histidine	0.01-100.0
		Cholesterol	0.001-200.0

The preparation method of the liposome anticreep hair cream that this embodiment provides is: 100mg phospholipid and 25mg cholesterol are dissolved in 30ml ethanol (or chloroform, methanol or dichloromethane etc.), even the solution I of vibrating, then put into eggplant-shape bottle, on water-bath Rotary Evaporators, constantly rotate under jolting, reduction vaporization desolventizes (35 DEG C ± 1 DEG C of bath temperature) to remove, and pass into every now and then nitrogen, until volatilization completely, then at room temperature dry 24 hours; Taking 3.0mg epidermal growth factor, 0.05mgSCGF (purchased from Peprotech company), 5mg fibroin, 5mg collagen protein, 20mg histidine, 25mg biotin and 25mg ginkgetin is dissolved in 40ml deionized water (or aqueous phosphatic PH=6.8) and on water-bath Rotary Evaporators constantly and shakes up to obtain aqueous solution II under rotation jolting; Then above-mentioned aqueous solution II is added in above-mentioned eggplant-shape bottle, on water-bath Rotary Evaporators, constantly under rotation jolting, obtain liposome emulsion, spy formula Ultrasound Instrument makes the abundant aquation 20-120 minute of lipid membrane under ice-water bath condition, until become transparently to emulsion, obtain carrying the liposome anticreep hair cream of an epidermal growth factor, human stem cell growth, fibroin, collagen protein, biotin and ginkgetin.

Fig. 2 is the schematic diagram of the skin absorption process of liposome anticreep hair cream provided by the invention.As shown in Figure 2, owing to sealing, the liposome adventitia of nutritional labeling is similar to human body cell outer membrane structure and composition, and therefore complete liposome can gap and the direct transdermal of skin subsidiary pipeline opening through horn cell, horn cell enter site of action.Liposome provides exogenous bilayer lipid membrane, make structural change between horn cell, hydrophobicity afterbody arrangement disorder in double-layer of lipoid, fat-soluble medicine can enter intercellular substance by diffusion and capillarity, horny layer humidifying and hydration are strengthened, thereby can be penetrated easily the horny layer of epidermis and arrive each position of skin.Liposome phospholipid and Stratum corneum lipids merge changes horny layer the Nomenclature Composition and Structure of Complexes, form a kind of flat grain structure, by lipid granule gap, liposomal encapsulated medicine or nutritional labeling are convenient to enter skin, via lipid exchange, fusion, safeguard the physiological function of skin, therefore the liposome of sealing nutritional labeling or medicine has the biomembranous characteristic of human body cell and function, affect the activity of cell, to participate in human body metabolism, maintain one of necessary basic substance of human normal physiological function, there is targeting as pharmaceutical carrier, long-lasting, pardon, lipotropy and hydrophilic.Because liposome has avirulence and immunogenicity, be applicable to biological vivo degradation, can protect the entrapped superficial growth factor and metallothionein, and can carry the nutritional labelings such as the superficial growth factor and fibroin and enter corium and subcutaneous tissue through horny layer.The nutritional labeling such as alleviation, controlled release superficial growth factor in corium and subcutaneous tissue.Because liposome has targeting, can improve the bioavailability at skin such as superficial growth factor.

The liposome anticreep hair cream that the present embodiment provides is on probation through 100 Mies of 30 years old to 45 years old, and after 20 days, 90% people's hair elasticity strengthens, pliability is good, and obviously no longer alopecia.

Embodiment 2

This embodiment provides a kind of liposome anticreep hair cream that increases hair elasticity, pliability, also can make hair restoration.Described liposome anticreep hair cream comprises the composition of the weight part ratio described in following table:

Composition	Concrete content mg/L
		Epithelical cell growth factor (EGF)	0.5-8.0
Human stem cell growth	0.01-0.1
		Lipoid	0.001-1000.0
Phospholipid	0.003-3000.0
		Fatty acid	0.001-1000.0
Biotin	0.001-100.0
		Ginkgetin	0.001-100.0
Fibroin	0.001-10.0
		Collagen protein	0.01-100.0
Histidine	0.01-100.0
		Vitamin E	0.001-200.0
Vitamin C	0.001-200.0
		Vitamin K	0.001-200.0
Cholesterol	0.001-200.0

The preparation method of the liposome anticreep hair cream that this embodiment provides is: by phosphatidase 11 00mg, lipoid 100mg, fatty acid 100mg, 25mg vitamin E, 25mg vitamin K and cholesterol 25mg, be dissolved in 40ml ethanol (or chloroform, methanol or dichloromethane etc.), even the solution I of vibrating, then put into eggplant-shape bottle, on water-bath Rotary Evaporators, constantly rotate under jolting, reduction vaporization desolventizes (35 DEG C ± 1 DEG C of bath temperature) to remove, and pass into every now and then nitrogen, until volatilization completely, then at room temperature dry 24 hours; Taking 3.0mg epidermal growth factor, 0.05mgSCGF (purchased from Peprotech company), 5mg fibroin, 5mg collagen protein, 20mg biotin, 20mg ginkgetin, 20mg histidine and 25mg vitamin C is dissolved in 50ml deionized water (or aqueous phosphatic PH=6.8) and on water-bath Rotary Evaporators constantly and shakes up to obtain aqueous solution II under rotation jolting; Then above-mentioned aqueous solution II is added in above-mentioned eggplant-shape bottle, on water-bath Rotary Evaporators, constantly under rotation jolting, obtain liposome emulsion, spy formula Ultrasound Instrument makes the abundant aquation 20-120 minute of lipid membrane under ice-water bath condition, until become transparently to emulsion, obtain carrying the liposome anticreep hair cream of the compositions such as an epidermal growth factor, human stem cell growth.Preferably, described phospholipid is lecithin.

The liposome anticreep hair cream that the present embodiment provides is on probation through 100 Mies of 30 years old to 45 years old, and after 10 days, 90% people's hair elasticity strengthens, pliability is good, and obviously no longer alopecia.

In addition, can liposome and solution separating be opened with SephadexG50 chromatographic column if needed.

Liposome anticreep hair cream provided by the invention is a kind of most advanced and sophisticated high-tech carrier for active principle, formed by phospholipid bimolecular, include the miniature vesicle of water purification, the quantitative nutritional labeling such as epidermal growth factor, human stem cell of energy encapsulation in vesicle, its average diameter is 100 nanometers, is 1/200～1/300 of human body cell diameter, can penetrate easily human body skin top layer, with cell fusion, performance effect.Therefore, liposome anticreep hair cream provided by the invention is the natural nutriment of hair follicle development, both can be used for treating natural on-off cycles of hair growth and grown the alopecia person who hinders, also can be used as the daily indispensable nursing product of hair healthy adult, for delaying cell aging, prevent the generation of excessive alopecia.

The using method of liposome anticreep hair cream provided by the invention:

1, after hair washing, this product is evenly spread upon on scalp and hair and stopped after 5 minutes, clear water is cleaned;

2 or temperature bathe 10 minutes effects better; 3, consumption is too much unsuitable, smears all and is as the criterion with skin and hair.

Below the effect that describes component of the present invention, preparation method in conjunction with the embodiments in detail and reach, but embodiment illustrates the present invention for demonstrating.Description is only for explaining claims.But protection scope of the present invention is not limited to description.In the technical scope that any those skilled in the art of being familiar with disclose in the present invention, the variation that can expect easily or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.

Claims

1. a liposome anticreep hair cream, prepare by the following method: 100mg phospholipid and 25mg cholesterol are dissolved in 30ml ethanol, chloroform, methanol or dichloromethane, even the solution I of vibrating, then put into eggplant-shape bottle, constantly rotate under jolting on water-bath Rotary Evaporators, reduction vaporization is to remove desolventizing, bath temperature is 35 DEG C ± 1 DEG C, and pass into every now and then nitrogen, until volatilization completely, then at room temperature dry 24 hours; Taking 3.0mg epidermal growth factor, 0.05mg human stem cell growth, 5mg fibroin, 5mg collagen protein, 20mg histidine, 25mg biotin and 25mg ginkgetin is dissolved in 40ml deionized water and on water-bath Rotary Evaporators constantly and shakes up to obtain aqueous solution II under rotation jolting; Then above-mentioned aqueous solution II is added in above-mentioned eggplant-shape bottle, on water-bath Rotary Evaporators, constantly under rotation jolting, obtain liposome emulsion, use spy formula Ultrasound Instrument under ice-water bath condition, to make the abundant aquation 20-120 minute of lipid membrane, until that emulsion becomes is transparent.

2. a liposome anticreep hair cream, prepare by the following method: by phosphatidase 11 00mg, lipoid 100mg, fatty acid 100mg, 25mg vitamin E, 25mg vitamin K and cholesterol 25mg, be dissolved in 40ml ethanol, chloroform, methanol or dichloromethane, even the solution I of vibrating, then put into eggplant-shape bottle, on water-bath Rotary Evaporators, constantly rotate under jolting, reduction vaporization is to remove desolventizing, bath temperature is 35 DEG C ± 1 DEG C, and pass into every now and then nitrogen, until volatilization completely, then at room temperature dry 24 hours; Taking 3.0mg epidermal growth factor, 0.05mg human stem cell growth, 5mg fibroin, 5mg collagen protein, 20mg biotin, 20mg ginkgetin, 20mg histidine and 25mg vitamin C is dissolved in 50ml deionized water and on water-bath Rotary Evaporators constantly and shakes up to obtain aqueous solution II under rotation jolting; Then above-mentioned aqueous solution II is added in above-mentioned eggplant-shape bottle, on water-bath Rotary Evaporators, constantly under rotation jolting, obtain liposome emulsion, use spy formula Ultrasound Instrument under ice-water bath condition, to make the abundant aquation 20-120 minute of lipid membrane, until that emulsion becomes is transparent.