CN102973586A - Fishery chitosan immunoenhancer - Google Patents
Fishery chitosan immunoenhancer Download PDFInfo
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- CN102973586A CN102973586A CN 201210493799 CN201210493799A CN102973586A CN 102973586 A CN102973586 A CN 102973586A CN 201210493799 CN201210493799 CN 201210493799 CN 201210493799 A CN201210493799 A CN 201210493799A CN 102973586 A CN102973586 A CN 102973586A
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- chitosan
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- immunoenhancer
- fishery
- purified water
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Abstract
The invention provides a fishery chitosan immunoenhancer. The fishery chitosan immunoenhancer is prepared by taking dilute acetic acid, purified water and chitosan as raw materials, wherein the concentration of the dilute acetic acid is 0.1mol/L, the volume ratio of the dilute acetic acid and the purified water is 1:1, the concentration of the chitosan is 0.02-0.08g/ml, and the chitosan is of chitosan oligosaccharide with molecular weight of lower than 60 thousand. The fishery chitosan immunoenhancer has the following technical effects of being capable of improving the non-specific immunity protective capability of aquatic animals, enhancing the antiviral infection and anti-bacteria infection capabilities of the aquatic animals, thereby controlling the occurrence and prevalence of diseases.
Description
Technical field
The present invention relates to a kind of fishing chitosan immunostimulant, belong to the aquaculture of aquatic animal technical field.
Background technology
Chitosan all has the effect of Promote immunity function to people, animal, plant.The positive chemoattractant of chitosan, absorbing mononuclear cell swims out of from blood vessel, be gathered in and form phagocyte in the tissue, or directly stimulate local organization, and promote hyperplasia, then develop into macrophage, therefore can activate body immune system, promote humoral immunization and cellular immune function, be typical B cell activator, can improve the activity of phagocytic function and the hydrolytic enzyme of macrophage.
In the prior art, a kind of chitosan product that is applicable to aquaculture of aquatic animal is arranged not yet.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, a kind of fishing chitosan immunostimulant is provided.
Fishing of the present invention chitosan immunostimulant, be that spirit of vinegar, purified water and the chitosan of 0.1mol/L is that preparation of raw material forms by concentration, the volume ratio of wherein said spirit of vinegar, purified water is 1:1, the concentration of described chitosan is 0.02-0.08g/ml, and described chitosan is that molecular weight is lower than 60,000 oligo-chitosan.
The concentration of described chitosan is 0.05g/ml.
Fishing of the present invention chitosan immunostimulant has following technique effect: can improve the nonspecific immunity protective capability of aquatic animal, improve the ability that aquatic animal viral infection resisting and antibacterial infect, thus the generation of control disease and popular.
The specific embodiment
Embodiment 1
Fishing chitosan immunostimulant, formulated by 50ml spirit of vinegar (concentration is 0.1mol/L), 50ml purified water and 2g chitosan, the concentration of chitosan is 0.02g/ml, chitosan is the oligo-chitosan of molecular weight 50,000.
Embodiment 2
Fishing chitosan immunostimulant, formulated by 50ml spirit of vinegar (concentration is 0.1mol/L), 50ml purified water and 5g chitosan, the concentration of chitosan is 0.05g/ml, chitosan is the oligo-chitosan of molecular weight 40,000.
Embodiment 3
Fishing chitosan immunostimulant, formulated by 50ml spirit of vinegar (concentration is 0.1mol/L), 50ml purified water and 8g chitosan, the concentration of chitosan is 0.08g/ml, chitosan is the oligo-chitosan of molecular weight 30,000.
Embodiment 4
Show with the immune effect experimental result of the oligo-chitosan among the embodiment 1-3 to Carassius aurutus gibelio, Penaeus vannamei and Eriocheir sinensis, the oligo-chitosan of interpolation doses has certain potentiation to rate of body weight gain and the Immune interrelation enzyme work such as ACP, CAT, lysozyme of Carassius aurutus gibelio, Penaeus vannamei and Eriocheir sinensis in the feedstuff, survival rate is 100%, wherein use the concentration mix fodder of 0.05g/ml to throw something and feed 3 courses for the treatment of, 7 days each courses for the treatment of, effect is best, and its effect is better than beta glucan.Carassius aurutus gibelio, Penaeus vannamei and Eriocheir sinensis rate of body weight gain respectively high by 30.4% than matched group, 69.4%, 37.6%, acid phosphatase (ACP) respectively high by 22.7% than matched group, 97.6%, 96.2%, catalase (CAT) respectively high by 14.0% than matched group, 6.7%, 72.9%, lysozyme respectively high by 43.9% than matched group, 14.7%, 26.6%
In addition, through application attestation in the culture of Chinese mitten crab, the test tank oligo-chitosan feedstuff of throwing something and feeding, morbidity obviously reduces, 45.24 kilograms of average yield per mus, the average rate of recapture 77.3%, 30.6 kilograms of matched group average yield per mus, the average rate of recapture 29.3%, use the test tank of oligo-chitosan, the crab sickness rate greatly reduces, and can improve the culture of Chinese mitten crab survival rate, increases culture benefit.Through bacterial diseasees such as cultivation Carassius auratus morbidity pond, Penaeus vannamei morbidity pond, Macrobrachium rosenbergii morbidity ponds preferably healing effect is arranged all simultaneously.Therefore, in the aquaculture of aquatic animal, oligo-chitosan has the nonspecific immunity protective capability that improves aquatic animal, and bacteriosis of aquatic livestock is had preferably preventive and therapeutic effect.
Claims (2)
1. fishing chitosan immunostimulant, it is characterized in that, be that spirit of vinegar, purified water and the chitosan of 0.1mol/L is that preparation of raw material forms by concentration, the volume ratio of wherein said spirit of vinegar, purified water is 1:1, the concentration of described chitosan is 0.02-0.08g/ml, and described chitosan is that molecular weight is lower than 60,000 oligo-chitosan.
2. fishing according to claim 1 chitosan immunostimulant is characterized in that, the concentration of described chitosan is 0.05g/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210493799 CN102973586A (en) | 2012-11-28 | 2012-11-28 | Fishery chitosan immunoenhancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210493799 CN102973586A (en) | 2012-11-28 | 2012-11-28 | Fishery chitosan immunoenhancer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102973586A true CN102973586A (en) | 2013-03-20 |
Family
ID=47848121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210493799 Pending CN102973586A (en) | 2012-11-28 | 2012-11-28 | Fishery chitosan immunoenhancer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102973586A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108821412A (en) * | 2018-07-02 | 2018-11-16 | 河南牧业经济学院 | A kind of improver of water quality and preparation method thereof for aquaculture |
| CN108911161A (en) * | 2018-07-02 | 2018-11-30 | 河南牧业经济学院 | A kind of water conditioner and preparation method thereof for aquaculture |
-
2012
- 2012-11-28 CN CN 201210493799 patent/CN102973586A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108821412A (en) * | 2018-07-02 | 2018-11-16 | 河南牧业经济学院 | A kind of improver of water quality and preparation method thereof for aquaculture |
| CN108911161A (en) * | 2018-07-02 | 2018-11-30 | 河南牧业经济学院 | A kind of water conditioner and preparation method thereof for aquaculture |
| CN108911161B (en) * | 2018-07-02 | 2021-04-16 | 河南牧业经济学院 | Water quality regulator for aquaculture and preparation method thereof |
| CN108821412B (en) * | 2018-07-02 | 2021-04-30 | 河南牧业经济学院 | Water quality improver for aquaculture and preparation method thereof |
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| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130320 |