CN102908459A - Anti-fatigue healthcare product or pharmaceutical composition and preparation method and application thereof - Google Patents
Anti-fatigue healthcare product or pharmaceutical composition and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides an anti-fatigue healthcare product or pharmaceutical composition and a preparation method and application thereof. The healthcare product or the pharmaceutical composition is prepared from, by weight, 50-90 parts of acanthopanax root, 30-50 parts of glossy ganoderma, 30-50 parts of wolfberry fruit and 60-110 parts of green tea. The healthcare product or the pharmaceutical composition is capable of effectively reducing organism serum urea nitrogen and blood lactic acid content and obviously prolonging load swimming time and has a good anti-fatigue effect. Besides, the healthcare product or the pharmaceutical composition is capable of increasing radiated mouse SOD (superoxide dismutase) activity, increasing white blood cells, lowering marrow micronucleus rate and increasing animal survival rate, has a remarkable anti-radiation function and is safe, non-toxic, good in taste and suitable for long-term taking.
Description
Technical field
The present invention relates to a kind of health product of resisting fatigue or pharmaceutical composition and its production and use.
Background technology
Along with the continuous quickening of people's rhythm of life, the problem of physical fatigue is perplexing popular crowd.When human body is under the fatigue state for a long time, can produce geromorphism and fatigue syndrome.Modern Chinese medicine thinks, fatigue is new sick kind that must pay attention to, is clinically commonly encountered diseases, frequently-occurring disease, and it is attributed to the subhealth state category, relates to the vital organs of the human body, mainly take spleen, liver, kidney as main.Can be by the medical treatment of Chinese medical discrimination prescription, to reach the purpose of prevention, treatment, control.
At present the traditional Chinese medical science is thought, fatigue is due to the deficiency syndrome and mental change (or not smooth) double factor of first QI consumed wound.Not only want the tonify deficiency righting in the treatment, tired the calming the nerves of also should dispelling.On the one hand, the tonify deficiency primordial QI reinforcing nourishes body, empty must the benefit, and canonical is prosperous, and is tired from removing; On the other hand, transfer smooth will, comfortable smooth meaning, refreshing must the peace, the heart is then peaceful, and fatigue can disappear.During Chinese traditional treatment, mainly be divided into following several respects:
1, control from spleen, replenishing QI to invigorate the spleen, tired the reposing of dispelling cures mainly the tired card of damage of the spleen
The tired card of damage of the spleen is many, and food and drink is excessive because of irregular diet, or sorrow ponders over too much, or it is heavy mixed and disorderly to work, dinner party frequently, due to the body forced labour.Can adopt Radix Panacis Quinquefolii or Radix Codonopsis, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Astragali Preparata, Rhizoma Dioscoreae, Fructus Amomi, Pericarpium Citri Reticulatae Viride, Pericarpium Citri Reticulatae, the Radix Aucklandiae, Endothelium Corneum Gigeriae Galli, Semen Raphani, Caulis Perillae, Herba Pogostemonis, Radix Glycyrrhizae etc. during treatment.Radix Panacis Quinquefolii or Radix Codonopsis, Rhizoma Atractylodis Macrocephalae (parched), Poria, Radix Astragali Preparata, Rhizoma Dioscoreae replenishing QI to invigorate the spleen in the side, activating the spleen during Fructus Amomi, Pericarpium Citri Reticulatae Viride, Pericarpium Citri Reticulatae, Radix Aucklandiae circulation of qi promoting are transferred, Caulis Perillae, Herba Pogostemonis removing dampness are amusing, Endothelium Corneum Gigeriae Galli, the Semen Raphani reason spleen that helps digestion, the Radix Glycyrrhizae QI invigorating and in.All medicines are played replenishing QI to invigorate the spleen altogether, the tired merit of reposing of dispelling.
2, control from liver, the yin nourishing easing the affected liver, the tired refreshment of dispelling cures mainly liver and hinders tired card
Liver hinder tired card many because of the food and drink dinner party too much, or operating pressure is large, or intemperance of emotions, considers excessively, or overfatigue, the change of Sentimental psychology surpasses due to the regulating power of liver.Nourishing the liver is the optimum selection of allaying tiredness and health preserving.Can adopt Radix Rehmanniae, Fructus Lycii, Fructus Corni, Radix Scrophulariae, Poria, Rhizoma Alismatis, Semen Ziziphi Spinosae, Radix Bupleuri, Radix Paeoniae Rubra, Flos Carthami, Radix Glycyrrhizae Preparata etc. during treatment.Radix Rehmanniae, Fructus Lycii, Fructus Corni, the nourishing the liver of Radix Scrophulariae YIN nourishing in the side; Poria, Rhizoma Alismatis damp eliminating spleen invigorating nourishing the liver, and get Poria and give birth to the wet merit of livering, the effect that the Rhizoma Alismatis diuretic expels the heat-evil; The Semen Ziziphi Spinosae yin nourishing is calmed the nerves; Radix Bupleuri soothing liver-QI gas is guiding drug; Radix Paeoniae Rubra, the Flos Carthami easing the affected liver of invigorating blood circulation is got the Flos Carthami nourishing blood to suppress the hyperactive liver, the soft liver of Radix Paeoniae Rubra removing heat from blood; The sweet Heibei provincial opera of Radix Glycyrrhizae Preparata is mended, and relaxes the property of medicine, coordinating the actions of various ingredients in a prescription.If wine the wounded increases Flos puerariae lobatae, Comprehensive relieving acute alcoholism and recuperating the spleen.All medicines are played the yin nourishing easing the affected liver altogether, the merit of the tired refreshment of dispelling.
3, control from the heart, the mind calming that nourishes heart, the tired benefit of dispelling is refreshing, cures mainly grieved tired card
Grieved tired card is how excessive because considering, and operating pressure is large, rhythm is fast, due to noise or the anxiety.Get Rhizoma Coptidis, Bulbus Lilii, Concha Margaritifera, Os Draconis, Concha Erosariae seu Cypraeae, Semen Ziziphi Spinosae, Cortex Albiziae, Poria cum Radix Pini, Radix Curcumae, Radix Bupleuri, Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Glycyrrhizae Preparata etc. during treatment.Rhizoma Coptidis, Bulbus Lilii clearing away heart-fire for tranquillization in the side, Concha Margaritifera, Os Draconis, Concha Erosariae seu Cypraeae tranquilizing mind, Semen Ziziphi Spinosae tranquilizing by nourishing the heart, Cortex Albiziae resolving stagnation for tranquilization, Poria cum Radix Pini's spleen-benefiting mind-tranquilizing, Radix Curcumae eliminating phlegm and relaxing heart, Radix Bupleuri soothing liver-QI for relieving depression, Rhizoma Chuanxiong, the Radix Salviae Miltiorrhizae smooth heart of invigorating blood circulation, Radix Glycyrrhizae Preparata mind tranquilizing and the heart calming, coordinating the actions of various ingredients in a prescription.All medicines are played the mind calming that nourishes heart altogether, tired benefit god's the merit of dispelling.
4, control from kidney, the kidney tonifying controlling nocturnal emission with astringent drugs is dispelled and is tiredly refreshed oneself, and cures mainly kidney and hinders tired card
Kidney is hindered the multifactor body of tired card and is possessed deficiency or for a long time labor prolonged illness, due to feeling exhausted.During treatment, can adopt Cordyceps 3 gram, Rhizoma Chuanxiong 5 grams, Herba Epimedii 10 grams, Herba Cistanches 12 grams, Rhizoma Smilacis Glabrae 15 grams, 1 of grass tortoise are 300 grams approximately, wherein, Cordyceps have mend the waist kidney, fall liver-fire, the effect for the treatment of lumbago and skelalgia, but all medicines share the kidney invigorating, invigorate blood circulation.
But above-mentioned all medicine flavour of a drug are more, and cost is higher, are unfavorable for the long term consumer use.At present, only yet there are no report with resisting fatigue behind Radix Et Caulis Acanthopanacis Senticosi, Fructus Lycii, Ganoderma, the green tea 4 flavor material combinations.
Summary of the invention
The object of the present invention is to provide a kind of health product or pharmaceutical composition with good antifatigue effect.Another object of the present invention is to provide preparation method and the purposes of these health product or pharmaceutical composition.
The invention provides a kind of health product or pharmaceutical composition of resisting fatigue, it is the preparation that the crude drug by following weight proportion is prepared from:
50 ~ 90 parts of Radix Et Caulis Acanthopanacis Senticosis, 30 ~ 50 parts of Ganodermas, 30 ~ 50 parts of Fructus Lycii, 60 ~ 110 parts of green tea.
Further: it is the preparation that the crude drug by following weight proportion is prepared from:
60 ~ 80 parts of Radix Et Caulis Acanthopanacis Senticosis, 35 ~ 45 parts of Ganodermas, 35 ~ 45 parts of Fructus Lycii, 75 ~ 95 parts of green tea.
Further preferably, it is the preparation that the crude drug by following weight proportion is prepared from:
70 parts of Radix Et Caulis Acanthopanacis Senticosis, 40 parts of Ganodermas, 40 parts of Fructus Lycii, 85 parts of green tea.
Wherein, described preparation is medicinal tea, granule, capsule, pill, tablet or powder.
Further, in the described medicinal tea, the 1g medicinal tea contains polysaccharide and is not less than 0.15g in fructose.
The present invention also provides the preparation method of above-mentioned health product or pharmaceutical composition, and it comprises following operating procedure:
(1) by the proportioning weighting raw materials;
(2) get Radix Et Caulis Acanthopanacis Senticosi, Ganoderma, Fructus Lycii, extracting in water, water extraction liquid is concentrated, after leaving standstill, filters, and gets supernatant for subsequent use;
(3) step (2) gained supernatant is sprayed on the green tea, after the drying, it is prepared into preparation pharmaceutically commonly used.
Further, in the step (2), relative density was 1.2 ~ 1.3 when water extraction liquid was concentrated into 80 ℃; In the step (3), described green tea is the green tea that has passed through 20 ~ 40 mesh sieves.
Further, in the step (3), the design parameter of described drying is: first 80 ~ 105 ℃ of bakings 2 hours, again in 105 ~ 110 ℃ of bakings 3 ~ 4 hours.
The present invention also provides above-mentioned health product or pharmaceutical composition preparing resisting fatigue or/and the purposes in radiation-resistant health product or the medicine.
Wherein, described health product or medicine are health product or the medicines with enhance SOD activity, function of increasing leukocyte.
Health product of the present invention or pharmaceutical composition can effectively reduce body serum urea nitrogen and Serum lactic acid content, obviously prolong the swimming with a load attached to the body time, have good antifatigue effect; Simultaneously, it can also increase raying mice superoxide dismutase (SOD) activity, increases the leukocyte effect, and can reduce Bone Marrow Micronuclear Rates, increase animal dis motility rate, have significant radiation-resisting functional, and safety non-toxic, good mouthfeel is suitable for long-term taking.
The specific embodiment
Among the present invention, described Radix Et Caulis Acanthopanacis Senticosi, Ganoderma, Fructus Lycii all meet " the relevant regulations in the Chinese pharmacopoeia 2010 editions; Described green tea meets the relevant regulations of GB/T14456 " green tea ".
The preparation method of embodiment 1 health product of the present invention or pharmaceutical composition
(1) by following prescription weighting raw materials:
Radix Et Caulis Acanthopanacis Senticosi 700g, Ganoderma 400g, Fructus Lycii 400g, green tea 850g;
(2) Radix Et Caulis Acanthopanacis Senticosi, Ganoderma, Fructus Lycii are added the water saturates heart, decocted 1.5 hours with 10 times of water gagings; Decocted 1.5 hours with 8 times of water gagings again, filter; Merge filtrate twice, left standstill 12 hours, filter, filtrate is concentrated into relative density 1.2 ~ 1.3(80 ℃ survey), placement is spent the night, and the siphon supernatant is crossed 350 eye mesh screens, gets clear paste and treats that spray uses;
(3) with the green tea screening, cross 20 ~ 40 eye mesh screens;
(4) spray: in 40 ~ 50 rev/mins of blenders that constantly roll, clear paste spray above-mentioned to be sprayed on the green tea surface, 80 ~ 105 ℃ of bakings 2 hours, in 105 ~ 110 ℃ of bakings 3 ~ 4 hours, is namely got medicinal tea again.
After measured, in the above-mentioned medicinal tea, polyoses content is counted 0.2323g ~ 0.2719g with fructose in every 1g medicinal tea, considers that the practical situation of large production and medical material transport, preserve the impact on polyoses content, and polyoses content is not less than 0.15g in fructose in tentative every 1g medicinal tea.
The preparation method of embodiment 2 health product of the present invention or pharmaceutical composition
Press following weight proportion weighting raw materials:
Radix Et Caulis Acanthopanacis Senticosi 900g, Ganoderma 300g, Fructus Lycii 300g, green tea 600g;
Press embodiment 1 method clear paste is sprayed after green tea surface, drying, pulverize, add suitable microcrystalline Cellulose, encapsulated behind the mixing, namely get capsule.
The preparation method of embodiment 3 health product of the present invention or pharmaceutical composition
Press following weight proportion weighting raw materials:
Radix Et Caulis Acanthopanacis Senticosi 500g, Ganoderma 500g, Fructus Lycii 500g, green tea 1100g.
Press embodiment 1 method clear paste is sprayed after green tea surface, drying, pulverize, add suitable dextrin, granulate, pack namely gets granule.
The preparation method of embodiment 4 health product of the present invention or pharmaceutical composition
Press following weight proportion weighting raw materials:
Radix Et Caulis Acanthopanacis Senticosi 600g, Ganoderma 450g, Fructus Lycii 450g, green tea 950g;
Press embodiment 1 method clear paste is sprayed after green tea surface, drying, pulverize, add suitable adjuvant, general ball namely gets pill.
The preparation method of embodiment 5 health product of the present invention or pharmaceutical composition
Press following weight proportion weighting raw materials:
Radix Et Caulis Acanthopanacis Senticosi 800g, Ganoderma 350g, Fructus Lycii 350g, green tea 750g;
Press embodiment 1 method clear paste is sprayed after green tea surface, drying, after the pulverizing, granulate, tabletting namely gets tablet.
Below specify beneficial effect of the present invention by test example.
Test example 1 present composition antifatigue effect
1 material
1.1 be subjected to reagent: get 45g medicinal tea (embodiment 1 preparation), add boiling water 450ml and soaked 30 minutes, repeatedly twice, Total Water is 900ml.60 ℃ of air blast of soak are concentrated into 270ml, and namely the 6ml concentrated solution is equivalent to 1 gram medicinal tea.
1.2 animal: bull Kunming mouse
2 experimental techniques
2.1 dosage grouping: mice is divided into 4 groups at random by body weight, i.e. distilled water matched group and medicinal tea 0.75g/kg, 1.5g/kg, three dosage groups of 3.0g/kg (be equivalent to respectively recommend the human body amount of quoting 5,10,20 times).Each group is all by 2% volume gavage every day, continuous 30 days.
2.2 testing index: after 30 minutes, carry out respectively Loaned swimming test, serum urea nitrogen and Serum lactic acid content and measure in the last gavage.
2.3 statistical analysis: adopt variance analysis and q check, use rank test during heterogeneity of variance.
3 results
3.1 Loaned swimming test: the results are shown in Table 1.
Statistic analysis result shows: the swimming with a load attached to the body time of three dosage groups of medicinal tea of the present invention all significantly is longer than matched group, and (0.75g/kg organizes P＜0.05; 1.5g/kg, 3.0g/kg organizes P＜0.01).
Table 1 Loaned swimming test result
Compare * P＜0.05, * * P＜0.01 with matched group
3.2 serum urea nitrogen content: the results are shown in Table 2.
Statistic analysis result shows, the serum urea nitrogen content of medicinal tea 1.5g/kg of the present invention, 3.0g/kg group significantly is lower than matched group (P＜0.01), and 0.75g/kg group and matched group comparing difference are without significance (P〉0.05).
Table 2 serum urea nitrogen content results
Compare *＜0.01 with matched group
3.3 the Serum lactic acid content measurement result sees Table 3.
Statistic analysis result shows, the Serum lactic acid content of three dosage groups of medicinal tea of the present invention all significantly is lower than matched group, and (1.5g/kg organizes P＜0.05; 0.75g/kg, 3.0g/kg organizes P＜0.01)
Table 3 Serum lactic acid content result
Compare * P＜0.05 * * P＜0.01 with matched group
4 brief summaries
The above experiment by an exercise test and two biochemical indicators shows, health product of the present invention or pharmaceutical composition can effectively reduce body serum urea nitrogen and Serum lactic acid content, obviously prolong the swimming with a load attached to the body time, illustrate that health product of the present invention or pharmaceutical composition have good antifatigue effect.
The health-care effect research of test example 2 health product of the present invention or pharmaceutical composition
One, materials and methods
1, sample treatment: get 50g medicinal tea (embodiment 1 preparation), adding boiling water 1000ml soaked after 3 hours, collect supernatant, adding boiling water 1000ml in the residue soaks after 3 hours and collects supernatant, repeat 3 times, merge 4 supernatant and be concentrated into 2000ml in 50 ± 2 ℃, every milliliter of concentrated solution is equivalent to medicinal tea 0.25g, puts Refrigerator store for subsequent use.Distilled water diluting becomes desired concn before use.
2, experimental animal: Kunming mouse
3, irradiation bomb:
60The Co gamma-rays
4, test method: choose body weight 22-28 gram Kunming mouse, be divided at random blank group, irradiation matched group and three health product of the present invention or pharmaceutical composition group (hereinafter to be referred as medicinal tea group of the present invention).Medicinal tea treated animal of the present invention respectively by 5,10,30 times dosage recommending the amount of drinking tea 9g/ people/day (0.75,1.5,4.5g/Kg body weight) gavage every day once, totally 5 weeks.Blank group, radiation control animals are used the distilled water gavage every day.4th week is used radiation matched group and three medicinal tea group experimental animals after gavage
60The Co gamma-rays carries out irradiation, and dosage is 1Gy/ time, once a day, and totally 10 times.Irradiation is given an example: 80cm, absorbed dose rate: 66.16cGy/min.20 hours statistics surviving animals numbers behind the last irradiation, put to death the animal extracting vein blood and measure superoxide dismutase (SOD) activity and leukocyte count (WBC), and get bone marrow of sternum by " foodsafety is independently learned assessment process " prescriptive procedure film-making, dyeing, microscopy.
Two, result of the test
See Table 4-7.Results of statistical analysis shows, medicinal tea 1.5g of the present invention and 4.5g/Kg dosage can increase raying mice superoxide dismutase (SOD) activity, and 4.5g/Kg can reduce its Bone Marrow Micronuclear Rates, increase the animal dis motility rate, and certain increase leukocyte effect is arranged.
Table 4 Determination of erythrocyte superoxide dismutase activity result
*: compare P＜0.05 with the blank group
*: compare P＜0.05 with the radiation matched group
Table 5 micronucleus test result
*: with blank group ratio religion P＜0.05
*: compare P＜0.05 with the radiation matched group
Table 6 leukocyte count measurement result
*: with blank group ratio religion P＜0.05
*: compare P＜0.05 with the radiation matched group
Table 7 mouse survival rate
*: with blank group ratio religion P＜0.05
*: compare P＜0.05 with the radiation matched group
Conclusion:
Above-mentioned experimental result as can be known, health product of the present invention or pharmaceutical composition can increase raying mice superoxide dismutase (SOD) activity, increase the leukocyte effect, and can reduce Bone Marrow Micronuclear Rates, increase animal dis motility rate, the result shows, health product of the present invention or pharmaceutical composition have significant radiation-resisting functional.
The toxicological study of test example 3 health product of the present invention or pharmaceutical composition
Tested medicine: the medicinal tea of getting embodiment 1 preparation is an amount of, the boiling water that adds 50 times of amounts soaks and filters and collect filtrate after 1 hour, and residue extracts twice with sending out to soak again, merge 3 soaked extracting solution in 60 ± 2 ℃ of vacuum-concentrcteds to desired concn, Refrigerator store is for subsequent use.
1, acute toxicity test
1.1 test method
40 of one-level Kunming mouses, 20 of one-level male SD rats; Mice is divided into 4 groups at random, and 10 every group, male and female half and half.Male SD rat is divided into 4 groups at random, 5 every group.Embodiment 1 medicinal tea dosage is respectively 1875,3750,7500 and 15000mg/Kg.bw.All by gavage of 1% volume, observed for two weeks after the contamination.
1.2 result of the test
Within the whole observation period, each contamination group is without animal dead, and also without unusual performance, namely medicinal tea of the present invention is to the acute oral LD of Kunming mouse and SD rat
50All greater than 15000mg/Kg.bw.
1.3 conclusion
According to the classification of food acute toxicity dosage, medicinal tea of the present invention is actual nontoxic level.
2, Salmonella reversion test
2.1 test method
Add and the dressing plate infiltration method that does not add rats'liver S9 with TA97, TA97, TA100 and four kinds of test strains of TA102.Medicinal tea of the present invention is established four dosage groups of 40,200,1000 and 5000 μ g/ wares, and feminine gender and positive control are established in each test simultaneously.The positive control that does not add S9 is 4-nitroquinoline oxide (NQNO), and the positive control that adds the S9 test is 2-amido fluorenes (2-AF).Each test dose is made three parallel wares, and repeated trials once.
2.2 result of the test sees the following form.
Table 8 Salmonella reversion test result
No matter add and the test that does not add S9, average the returning of positive control ware becomes the bacterium colony number average greater than the twice of negative control; And average the returning of each dosage group of medicinal tea of the present invention becomes the bacterium colony number average in the twice of negative control.
2.3 conclusion
Medicinal tea of the present invention is negative result in Salmonella reversion test, namely without mutagenic action.
3, mouse marrow cell micro nuclear test
3.1 test method
50 of one-level Kunming mouses, male and female half and half are divided into 5 groups at random.I.e. three medicinal tea groups of the present invention (1250,2500 and 5000mg/Kg.bw), a negative control group (distilled water) and a positive controls (cyclophosphamide 40mg/Kg.bw).Respectively at 0h and twice per os gastric infusion of 24h, 30h puts to death mice, gets the bone marrow of sternum picture, fixing rear Giemsa dyeing, and every Mus is observed 1000 polychromatic erythrocytes, the cell number of record paper micronucleus under the mirror.Diversity with micronuclear rates between each tested group of t check analysis and matched group.
3.2 the results are shown in following table.
Table 9 mouse microkernel test result
* compare P＜0.01 with negative control.
Statistic analysis result, the average micronucleus of positive controls are apparently higher than negative control (P＜0.01), and the micronuclear rates of medicinal tea group of the present invention and negative control are relatively without significant difference.
3.3 conclusion
Medicinal tea of the present invention is negative result in mouse marrow cell micro nuclear test.
4, mouse sperm deformity test
4.1 test method
Male mice in kunming, 5 every group, totally 5 groups.I.e. three medicinal tea groups of the present invention (1250,2500 and 5000mg/Kg.bw), a negative control group (distilled water) and a positive controls (cyclophosphamide 40mg/Kg.bw).Every day, gavage was given tested medicine, continuously 5d.35d puts to death animal after gavage first, gets the bilateral epididymis and makes the sperm smear, fixing after Yihong dyeing.The Microscopic observation sperm morphology, every animal is observed 1000 complete sperms, records all kinds of teratospermia numbers, calculates rate of teratosperm.
4.2 result of the test sees the following form
Table 10 sperm malformation test result
* compare P＜0.01 with negative control
The average rate of teratosperm of positive controls is apparently higher than negative control (P＜0.01), and each group of medicinal tea of the present invention compares with negative control, the difference not statistically significant.
4.3 conclusion
Medicinal tea of the present invention is negative result in the mouse sperm deformity test.
5 tertogenicity tests
5.1 test method
75 of the female sd inbred rats of sexual maturity and not copulation, 25 of male SD rats.Mate by 2:1 and to spend the night, make vaginal smear examination morning next day, looks into the person that sees the sperm and be considered as copulation and location become pregnant " 0 " day.Pregnant Mus is divided into 4 groups, i.e. three medicinal tea groups of the present invention (1250,2500 and 5000mg/Kg.bw), a negative control group (distilled water), 10 every group.In 7 ~ 16 days pregnancy periods per os gastric infusion, gestation pregnant Mus of Femur blood vessel sacrificed by exsanguination on the 20th makes fetal toxicity and teratogenecity and detects.
5.2 result of the test sees the following form.
Table 11 medicinal tea of the present invention is on the impact of rat motherhood situation and fetal development
Table 12 medicinal tea of the present invention is on the impact of rat embryo internal organs and skeleton deformity
Annotate: be lopsided percentage rate in the bracket
The result shows, each dosage group of medicinal tea of the present invention and negative control group are relatively, give a birth pregnant Mus number, average every nest tire number, absorb tire and the indexs such as still birth rate, embryo's body weight and body are long, tail is long, embryo's internal organs and skeleton deformity incidence rate all without significant difference, show medicinal tea of the present invention to rat without fetal toxicity and teratogenecity.
5.3 conclusion
Medicinal tea of the present invention is negative result in the Teratogenicity test.
6 30 days feeding trials
6.1 test method
Use 80 of one-level SD rats, be divided into 4 groups, be i.e. three medicinal tea groups of the present invention (1250,2500 and 5000mg/Kg.bw), a negative control group (distilled water), 20 every group, male and female half and half.Tested material was mixed the forage feed animal 30 days, make clinical examination, internal organs weighing, hematology and blood biochemical by the regulation of GB15193.13-94 and learn inspection, histopathologic examination.
6.2 result of the test sees the following form.
Table 13 medicinal tea of the present invention is on the impact (n=20) of rat average weight gain and food utilization
Table 14 medicinal tea of the present invention is learned the impact (n=20) of index on rat blood
Table 15 medicinal tea of the present invention is on the impact (n=20) of rat biochemical indicator
Table 16 medicinal tea of the present invention is on the impact (n=20) of Rats Organs and Tissues coefficient
Above-mentioned experiment shows, rat was fed through 30 days, each dosage group growth promoter of medicinal tea of the present invention is good, body weight gain, food utilization, hematological indices, biochemical indicator and organ coefficient are showed no unusually, each internal organs gross anatomy observation and pathological anatomy observe internal organs such as showing the heart, liver, spleen, lung, kidney, adrenal gland, thymus, thyroid, gastrointestinal, testis and ovary and negative control group compares, and all finds obviously unusual and toxic pathological changes.
6.3 conclusion
The maximal non-toxic Dosages of medicinal tea of the present invention in 30 days feeding trials of rat〉5000mg/Kg.
7 brief summaries
Medicinal tea of the present invention belongs to the avirulence product, has good oral administration safety.
In sum, health product of the present invention or pharmaceutical composition can effectively reduce body serum urea nitrogen and Serum lactic acid content, obviously prolong the swimming with a load attached to the body time, have good antifatigue effect; Simultaneously, it can also increase raying mice superoxide dismutase (SOD) activity, increases the leukocyte effect, and can reduce Bone Marrow Micronuclear Rates, increase animal dis motility rate, have significant radiation-resisting functional, and safety non-toxic, good mouthfeel is suitable for long-term taking.
Claims (10)
1. the health product of a resisting fatigue or pharmaceutical composition is characterized in that: it is the preparation that the crude drug by following weight proportion is prepared from:
50 ~ 90 parts of Radix Et Caulis Acanthopanacis Senticosis, 30 ~ 50 parts of Ganodermas, 30 ~ 50 parts of Fructus Lycii, 60 ~ 110 parts of green tea.
2. health product according to claim 1 or pharmaceutical composition is characterized in that: it is the preparation that the crude drug by following weight proportion is prepared from:
60 ~ 80 parts of Radix Et Caulis Acanthopanacis Senticosis, 35 ~ 45 parts of Ganodermas, 35 ~ 45 parts of Fructus Lycii, 75 ~ 95 parts of green tea.
3. health product according to claim 1 or pharmaceutical composition is characterized in that: it is the preparation that the crude drug by following weight proportion is prepared from:
70 parts of Radix Et Caulis Acanthopanacis Senticosis, 40 parts of Ganodermas, 40 parts of Fructus Lycii, 85 parts of green tea.
4. according to claim 1-the 3 described health product of any one or pharmaceutical composition, it is characterized in that: described preparation is medicinal tea, granule, capsule, pill, tablet or powder.
5. health product according to claim 4 or pharmaceutical composition, it is characterized in that: in the described medicinal tea, the 1g medicinal tea contains polysaccharide and is not less than 0.15g in fructose.
6. the preparation method of the described health product of claim 1-3 any one or pharmaceutical composition, it is characterized in that: it comprises following operating procedure:
(1) by the proportioning weighting raw materials;
(2) get Radix Et Caulis Acanthopanacis Senticosi, Ganoderma, Fructus Lycii, extracting in water, water extraction liquid is concentrated, after leaving standstill, filters, and gets supernatant for subsequent use;
(3) step (2) gained supernatant is sprayed on the green tea, after the drying, it is prepared into preparation pharmaceutically commonly used.
7. preparation method according to claim 6, it is characterized in that: in the step (2), relative density was 1.2 ~ 1.3 when water extraction liquid was concentrated into 80 ℃; In the step (3), described green tea is the green tea that has passed through 20 ~ 40 mesh sieves.
8. preparation method according to claim 6, it is characterized in that: in the step (3), the design parameter of described drying is: first 80 ~ 105 ℃ of bakings 2 hours, again in 105 ~ 110 ℃ of bakings 3 ~ 4 hours.
9. the described health product of claim 1-5 any one or pharmaceutical composition are preparing resisting fatigue or/and the purposes in radiation-resistant health product or the medicine.
10. purposes according to claim 9, it is characterized in that: described health product or medicine are health product or the medicines with enhance SOD activity, function of increasing leukocyte.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104244877A CN102908459A (en) | 2012-10-30 | 2012-10-30 | Anti-fatigue healthcare product or pharmaceutical composition and preparation method and application thereof |
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| CN103932185A (en) * | 2014-04-21 | 2014-07-23 | 信阳师范学院 | Compound chewable tablet containing dendrobium officinale, cordyceps militaris, ganoderma lucidum and tea leaves and preparation method thereof |
| CN104352749A (en) * | 2014-09-29 | 2015-02-18 | 于惊涛 | Drug or food composition with antagonistic effect on toxicity of organic pollutants in environment |
| CN104382030A (en) * | 2014-12-04 | 2015-03-04 | 高秀媛 | Healthcare food for adjusting liver and kidney function and preparation process of healthcare food |
| CN105029394A (en) * | 2014-04-15 | 2015-11-11 | 四川万安石斛产业开发有限公司 | Anti-fatigue health-caring product or medicine composition |
| CN106387596A (en) * | 2016-08-31 | 2017-02-15 | 南京禾宇化工有限公司 | Effervescent tablets capable of refreshing mind and restoring consciousness and preparation method of effervescent tablets |
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| CN104382030A (en) * | 2014-12-04 | 2015-03-04 | 高秀媛 | Healthcare food for adjusting liver and kidney function and preparation process of healthcare food |
| CN106387596A (en) * | 2016-08-31 | 2017-02-15 | 南京禾宇化工有限公司 | Effervescent tablets capable of refreshing mind and restoring consciousness and preparation method of effervescent tablets |
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Application publication date: 20130206 |