CN102488743A - Chinese traditional medicine composition, preparation method thereof and Chinese traditional medicine preparation - Google Patents
Chinese traditional medicine composition, preparation method thereof and Chinese traditional medicine preparation Download PDFInfo
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Abstract
The invention relates to the field of Chinese traditional medicines, and in particular relates to a Chinese traditional medicine composition, a preparation method thereof and a Chinese traditional medicine preparation. The total efficiency ratio for treating nephritis by virtue of the Chinese traditional medicine composition and the Chinese traditional medicine preparation reaches above 90%, and the Chinese traditional medicine composition and the Chinese traditional medicine preparation have the efficacies of tonifying kidney and spleen and removing blood stasis, can be used for treating nephritis, are stable in treatment effect, are innoxious and have no side effect, and are suitable for being taken for long term.
Description
Technical field
The present invention relates to the field of Chinese medicines, particularly a kind of Chinese medicine composition, method for preparing and Chinese medicine preparation.
Background technology
According to the statistics of World Health Organization (WHO), the public health that national governments carry out for the nephropathy patient drops into year by year and increases, and patient itself also will stand huge slight illness and bear huge economy expenditure simultaneously.Nephritis is nephropathy patient's principal element, is to cause the patient finally to develop into the primary factor of chronic renal failure in China.Study of evidence based medicine confirms that the kidney pathology extent of damage and decreased renal function degree are the principal elements of decision CKD patients'prognosis.Nearly 100 people get into the chronic renal failure phase in annual per 1,000,000 crowds of China according to statistics.This wherein nephritis can not get actively and efficacious therapy is wherein important link.The financial burden of nephritis serious threat human health and increase entire society is unfavorable for social development and human progress.
Kidney is a body excretion metabolism refuse, the vitals of keeping water, electrolyte and acid-base balance and producing multiple hormone.Thereby it is stable to keep organismic internal environment, and metabolism is normally carried out.Nephritis is from the viewpoint of modern medicine; Mainly be by this inflammatory reaction under immune-mediated situation of body; After a variety of causes caused antigen (like infected by microbes) to get into human body, body produced corresponding antibody, was combined into immune complex arrived and be deposited on kidney along with blood circulation different parts; The pathology damage that causes forms different nephritis types.Mainly be divided into acute nephritis and chronic nephritis clinically from the morbidity course of disease, general many sick concealments of chronic nephritis, state of an illness protracted course of disease often, patient's time more than half a year, is called chronic nephritis at most.Chronic nephritis clinical have albuminuria, hematuria and hypertension and renal function damages in various degree if the not good meeting of the state of an illness control renal failure occurs after several months or several years more.Cause by factors such as infection and the acute nephritis onset is many, clinical symptoms show many water breakthroughs swollen obvious, blood pressure increases, oliguria, urine protein and middle red blood cell of urine increase.Though acute nephritis is different with the chronic nephritis pathogenic factor, cause the machine-processed similar of kidney pathology damage.No matter any nephritis; If the state of an illness can not in time be corrected; The decline of carrying out property of renal function possibly cause renal failure, finally is developed to uremic performance, and uremia is the total clinical syndrome of nephropathy in various late periods; It is the final stage of carrying out property chronic renal failure; At this moment, human body causes General Symptoms owing to a large amount of in vivo retentions of uremic toxins present a plurality of visceral organ injuries such as digestion, circulation, breathing, nerve, muscle, blood except water, metabolic disturbance of electrolyte and acid base imbalance.
Chinese medicine thinks that the physiological function of kidney mainly comprises main growth promoter, main reproduction, the metabolism of main water liquid, the living medullization blood of main bone store essential substances, main improving inspiration by invigorating kidney-QI and moistens aspects such as supporting warm internal organs.These functions are equivalent to the part physiological function of urinary system in the modern medicine, reproduction, endocrine, nerve, blood and respiratory system.The physiological function of kidney is kidney essense, kidney qi, kidney yin, the coefficient result of kidney yang.That the essence of kidney, gas, yin, yang derive from is congenital, fill and contained the day after tomorrow, and promptly they derive from congenitally on the one hand, possess in father and mother; Simultaneously after birth; Depend on the nourishing of each essences of ZANG FU-organs gas negative and positive again,, keep normal physiological function to keep the Sheng of filling of essence in kidney negative and positive.Middle red blood cell of urine and albumen think that the outer of body cereal nutrient rushes down.Mainly be to cause envelope to be hidden admittedly because the kidney vital essence is lost void, vital essence leaks, and can not collect the essence of five ZANG-organs and six FU-organs gas, produces albuminuria and hematuria.
CN 03114205 discloses a kind of Chinese medicine composition of treating nephritis, and its Herba Epimedii, Rhizoma Curculiginis, crust halberd, the Cortex Eucommiae, Semen Cuscutae, Cornu Cervi Degelatinatum, Radix Panacis Quinquefolii, Radix Stephaniae Tetrandrae, the Rhizoma Anemarrhenae, Cortex Phellodendri, Radix Notoginseng, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Ganoderma are processed.CN 02148937 discloses a kind of Chinese medicine composition of treating chronic nephritis, and said composition is made up of the Radix Astragali, Poria, Herba Epimedii, Bombyx Batryticatus, Scorpio, Rhizoma Alismatis, Folium Pyrrosiae, Caulis Sinomenii.CN200410051010 discloses a kind of Chinese medicine composition of treating nephritis, and said composition is processed by Fructus Foeniculi, Folium Bambusae, Medulla Junci, Radix Stephaniae Tetrandrae, Fel Sus domestica.CN 200710115295 discloses a kind of Chinese medicine composition that is used to treat chronic nephritis, and said composition is formulated by the Radix Astragali, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Herba Leonuri, Rhizoma Dioscoreae, Pericarpium Arecae, Rhizoma Alismatis, Radix Aconiti Lateralis Preparata, Periostracum Cicadae.CN 200710115279 discloses a kind of Chinese medicine composition that is used to treat chronic nephritis, said composition by the Radix Rehmanniae, Carapax et Plastrum Testudinis, Fructus Corni, Cortex Moutan, Radix Achyranthis Bidentatae, the Radix Astragali, Fructus Lycii, Cordyceps, Poria, Herba Ephedrae, Cortex Mori, the Rhizoma Atractylodis Macrocephalae, windproof, Rhizoma Dioscoreae is formulated.CN200610124864 discloses a kind of treatment nephritis and uremic Chinese medicine composition, and said composition is processed by Radix humuli scandentis, Spora Lygodii, Radix Serissae, Castanea seguinii Dode (Involucrum Castaneae Seguinii), Rhizoma Phyllostachydis henonis and Pericarpium Lagenariae (pericarpium Lagenariae sicerariae).CN 200610011864 discloses a kind of Chinese medicine composition of treating chronic nephritis.This Chinese medicine composition is processed by Radix Ginseng, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Poria, Rhizoma Alismatis, Rhizoma Pinelliae, Rhizoma Et Radix Notopterygii, Radix Angelicae Pubescentis, windproof, Radix Bupleuri, Rhizoma Coptidis, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae, Radix Glycyrrhizae Preparata, Rhizoma Zingiberis Recens and Fructus Jujubae.CN 200710012175 discloses a kind of Chinese medicine composition that is used to treat nephritis, is processed by phellodendron bark, CORTEX ACANTHOPANACIS, Cortex Mori, Cortex Zingiberis, Cortex Sclerotii Poriae, Pericarpium Arecae, Pericarpium Citri Reticulatae.CN200710115606 discloses a kind of Chinese medicine composition that is used to treat chronic nephritis, and said composition is formulated by the Radix Astragali, Poria, Rhizoma Dioscoreae, Rhizoma Cibotii, Fructus Lycii, Pericarpium Citri Reticulatae, Semen Plantaginis, Radix Morindae Officinalis, Cornu Cervi, Fructus Corni.CN 200610165478 discloses a kind of Chinese medicine composition that is used for chronic nephritis, and it is processed by Radix Flemingiae Philippinensis, Herba Epimedii, Fructus Psoraleae, Cordyceps, Radix Ginseng Rubra, Rhizoma Polygonati, Radix Polygoni Multiflori Preparata, Radix Rehmanniae, Radix araliae armatae, Radix Notoginseng, Radix Et Rhizoma Rhei, Semen sojae atricolor, Rhizoma Smilacis Glabrae, Herba Leonuri, Rhizoma Alismatis, Rhizoma Imperatae.
Although it is simple that above-mentioned Chinese medicine composition has preparation; Characteristics such as easy to use, but the medicine of above-mentioned Chinese medicine composition is too much, and individual drug costs an arm and a leg; Medical material is rare; Prescription is excessive, and to cause making when becoming Chinese patent medicine in the per unit pharmaceutical composition active constituent content less, not only cause the waste in medicine source, the increase of patient's expenditure, and drug effect is not high; Botanical, animality medical material are different because of concocting method when the preparation Chinese medicine composition in the pharmaceutical composition, and effective ingredient is very easily loss in the preparation process, and the quality control of Chinese medicine composition is difficult to grasp.
Therefore, provide a kind of Chinese medicine composition, method for preparing and Chinese medicine preparation to have realistic meaning.
Summary of the invention
In view of this, the present invention provides a kind of Chinese medicine composition, method for preparing and Chinese medicine preparation.This Chinese medicine composition and Chinese medicine preparation are processed by four kinds of Chinese crude drugs, and the total effective rate of treatment nephritis reaches more than 96%, has the kidney tonifying spleen invigorating, and the effect of blood circulation promoting and blood stasis dispelling can be used for the treatment of nephritis, and stable curative effect, has no side effect, and is suitable for taking for a long time.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme:
The invention provides a kind of Chinese medicine composition of treating nephritis, it is characterized in that, process by following weight portion raw material:
The Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions.
Medical angle therefrom, urine protein are the pathological products that human body is precise and tinyly made a bet, vital essence leaks, and belong to Chinese medicine " smart (gas) ", " precise and tiny " category.The kidney master closes store essential substances gas, and the kidney qi abundance then can be regulated body " smart (gas) " the whole body organ of supplementing nutrition.The renal function imbalance, then precise and tiny making a bet, vital essence leaks, and forms urine protein.Therefore, should serve as that main discussing controlled nephritis with " kidney " in theory.Its pathogenesis mainly is that pathological changes is initial, and kidney loses void and causes closing store essential substances gas mistake department and cause not store essential substances of kidney, and vital essence leaks; Along with pathological changes is goed deep into, it is unfavorable that the vital essence consume causes the not handsome blood of gas the blood vessels operation to occur.The blood vessels stasis of blood stagnate cause that the venation impatency increases the weight of and cause a disease deepen into, therefore in the nephritis pathological process, pay attention to supplementing the kidney to control the nocturnal and utilize one's abilities with uniting of blood circulation promoting and blood stasis dispelling and on treating, obtains ideal curative effect.The glomerule venation is fine, meets the be right category of " grandson's network " of science of arteries and veins, and channels is fine to be easy to stasis of blood resistance, and stasis of blood resistance more very thereby with the theory of blood circulation promoting and blood stasis dispelling runs through treatment all the time in the pathological process.
The Chinese crude drug Radix Astragali is the root of leguminous herbaceous plant's Radix Astagali, Radix Astragali, has effects such as invigorating QI to consolidate the body surface resistance, diuretic detumescent, expelling pus and toxin by strengthening QI, granulation promoting.Modern study, the Radix Astragali contain various trace elements such as Saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.The enhancing human body immunity function arranged, protect the liver, diuresis, defying age, anti-stress, blood pressure lowering and antibacterial action more widely.As preferably, select Inner Mongolia Astragalis in the some embodiments of the present invention for use.
When the dry root that is classified as umbelliferae angelica, can enrich blood, can invigorate blood circulation again, both can stimulate the menstrual flow, again can be active.As preferably, select Gansu Min Radix Angelicae Sinensis in the some embodiments of the present invention for use.
The Chinese crude drug Radix Achyranthis Bidentatae is the root of amaranthaceous plant Radix Achyranthis Bidentatae.Cure mainly soreness of waist and knee joint, flaccidity and weakness of the lower limbs, amenorrhea due to stagnation of blood, dysmenorrhea, puerperal abdominal pain due to blood stasis, lump in the abdomen, retention of placenta, pyretic stranguria, stranguria with blood, traumatic injury, carbuncle malignant boil, laryngopharynx swelling and pain.As preferably, in the some embodiments of the present invention, select Radix Achyranthis Bidentatae for use.
The Chinese crude drug Rhizoma Chuanxiong is a samphire Rhizoma Chuanxiong rhizome.Cure mainly menoxenia, amenorrhea dysmenorrhea, puerperal the stasis of blood stagnate that raw meat pain, lump in the abdomen lump, pain in chest and hypochondrium, headache are dizzy, anemofrigid-damp arthralgia, traumatic injury, ulcer sores.As preferably, in the some embodiments of the present invention, select Szechwan Ligusticum wallichii for use.
In embodiment more provided by the invention, the raw material of Chinese medicine composition provided by the invention comprises: the Radix Astragali 5～60 weight portions, Radix Angelicae Sinensis 1～12 weight portion, Radix Achyranthis Bidentatae 2.5～30 weight portions, Rhizoma Chuanxiong 2～20 weight portions.
In other embodiment provided by the invention, the raw material of Chinese medicine composition provided by the invention comprises: the Radix Astragali 15～60 weight portions, Radix Angelicae Sinensis 3～12 weight portions, Radix Achyranthis Bidentatae 7.5～30 weight portions, Rhizoma Chuanxiong 5～20 weight portions.
The present invention also provides a kind of method for preparing of treating the Chinese medicine composition of nephritis, and raw material comprises the Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions;
Get Radix Angelicae Sinensis, Rhizoma Chuanxiong residual liquid after vapor distillation is collected volatile oil and distilled, get first medicinal residues and add water through first decoction, filter back collection first filtrating, said first filtrates mixes the concentrated clear paste of winning in back with said distillation back residual liquid; Said first clear paste is 1.10～1.20 60～80 ℃ of following relative densities;
Get the Radix Astragali, Radix Achyranthis Bidentatae through 60～95% alcohol heating reflux, collect concentrate behind the extracting solution second clear paste; Get second medicinal residues and add water and decoct through second, filter the back collect second filtrating concentrate the 3rd clear paste; Said second clear paste or said the 3rd clear paste are 1.10～1.20 60～80 ℃ of following relative densities;
Get said first clear paste, said second clear paste mixes with said the 3rd clear paste, makes extractum;
Get said extractum and mix, promptly get with said volatile oil.
In the method for preparing of Chinese medicine composition provided by the invention, collect volatile oil, get Chinese medicine composition provided by the invention, kept the active constituents of medicine in the Chinese crude drug to greatest extent with above-mentioned extractum combination system.
As preferably, said vapor distillation is specially vapor distillation 1～2 time, each 2～8h.
As preferably, said first decocts and is specially, and in g/mL, the mass volume ratio of said first medicinal residues and water is 1: 6～12.
As preferably, said backflow is specially and refluxes each 1～3h 1～3 time.
As preferably, said second decocts and is specially, and in g/mL, the mass volume ratio of said second medicinal residues and water is 1: 6～12.
The Chinese medicine composition that the present invention also provides above-mentioned method for preparing to make.
The present invention also provides a kind of Chinese medicine preparation of treating nephritis, is processed by above-mentioned Chinese medicine composition and acceptable accessories.
In embodiment more provided by the invention, Chinese medicine preparation provided by the invention can be granule, capsule, tablet, pill or oral liquid.
The present invention provides a kind of Chinese medicine composition, method for preparing and Chinese medicine preparation.This Chinese medicine composition and Chinese medicine preparation select for use four kinds of raw materials to process: the Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions.Not only practice thrift the medicine source, reduced patient's expenditure, and the test of pesticide effectiveness shows; The total effective rate of Chinese medicine composition provided by the invention and Chinese medicine preparation treatment nephritis reaches more than 96%, has the kidney tonifying spleen invigorating, the effect of blood circulation promoting and blood stasis dispelling; Can be used for the treatment of nephritis; And stable curative effect, have no side effect, be suitable for taking for a long time.The method for preparing of Chinese medicine composition provided by the invention; Adopt and collect volatile oil, vinasse, alcohol reflux liquid, medicinal residues decocting liquid respectively; Above-mentioned vinasse, alcohol reflux liquid, the merging of medicinal residues decocting liquid are got extractum after concentrating; Merge with volatile oil then, make Chinese medicine composition provided by the invention, kept the active constituents of medicine in the Chinese crude drug to greatest extent.
Description of drawings
Fig. 1 shows that kidney of rats organizes HE dyeing light microscopic picture; Wherein, Fig. 1 (A) shows the treatment group, and Fig. 1 (B) shows matched group;
Fig. 2 shows that kidney of rats organizes MMP-9 immunohistochemistry picture; Wherein, Fig. 2 (A) shows the treatment group, and Fig. 2 (B) shows matched group;
Fig. 3 shows that kidney of rats organizes Col-IV immunohistochemistry picture; Wherein, Fig. 3 (A) shows the treatment group, and Fig. 3 (B) shows matched group;
Fig. 4 shows kidney of rats tissue T GF-β 1 immunohistochemistry picture; Wherein, Fig. 4 (A) shows the treatment group, and Fig. 4 (B) shows matched group;
Fig. 5 shows kidney of rats tissue T IMP-1 immunohistochemistry picture; Wherein, Fig. 5 (A) shows the treatment group, and Fig. 5 (B) shows matched group.
The specific embodiment
The invention discloses a kind of Chinese medicine composition, method for preparing and Chinese medicine preparation, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as and are included in the present invention.Method of the present invention and application are described through preferred embodiment; The related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use technology of the present invention.
The invention provides a kind of Chinese medicine composition of treating nephritis, it is characterized in that, process by following weight portion raw material:
The Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions.
In embodiment more provided by the invention, the raw material of Chinese medicine composition provided by the invention comprises: the Radix Astragali 15～60 weight portions, Radix Angelicae Sinensis 3～12 weight portions, Radix Achyranthis Bidentatae 7.5～30 weight portions, Rhizoma Chuanxiong 5～20 weight portions.
The present invention also provides a kind of method for preparing of treating the Chinese medicine composition of nephritis, and raw material comprises the Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions;
Get Radix Angelicae Sinensis, Rhizoma Chuanxiong residual liquid after vapor distillation is collected volatile oil and distilled, get first medicinal residues and add water through first decoction, filter back collection first filtrating, said first filtrates mixes the concentrated clear paste of winning in back with said distillation back residual liquid; Said first clear paste is 1.10～1.20 60～80 ℃ of following relative densities;
Get the Radix Astragali, Radix Achyranthis Bidentatae through 60～95% alcohol heating reflux, collect concentrate behind the extracting solution second clear paste; Get second medicinal residues and add water and decoct through second, filter the back collect second filtrating concentrate the 3rd clear paste; Said second clear paste or said the 3rd clear paste are 1.10～1.20 60～80 ℃ of following relative densities;
Get said first clear paste, said second clear paste mixes with said the 3rd clear paste, makes extractum;
Get said extractum and mix, promptly get with said volatile oil.
As preferably, said vapor distillation is specially vapor distillation 1～2 time, each 2～8h.
As preferably, said first decocts and is specially, and in g/mL, the mass volume ratio of said first medicinal residues and water is 1: 6～12.
As preferably, said backflow is specially and refluxes each 1～3h 1～3 time.
As preferably, said second decocts and is specially, and in g/mL, the mass volume ratio of said second medicinal residues and water is 1: 6～12.
The Chinese medicine composition that the present invention also provides above-mentioned method for preparing to make.
The present invention also provides a kind of Chinese medicine preparation of treating nephritis, is processed by above-mentioned Chinese medicine composition and acceptable accessories.
In embodiment more provided by the invention, Chinese medicine preparation provided by the invention can be granule, capsule, tablet, pill or oral liquid.
The selection foundation of the test of pesticide effectiveness:
Selecting male SD rat for use according to the document relevant report is that to carry out the chronic nephritis animal model experiment be the animal experiment method of generally acknowledging at present to laboratory animal.Use the tail vein injection method, use amycin to calculate disposable injection 0.06% amycin of tail vein according to per kilogram of body weight 6mg.Observations that experimentize of preparation mRNA IN ADRIAMYCIN NEPHROPATHY animal model, the safety and the therapeutic effect of understanding medicine.This method is one of research nephritis curative effect animal model the most commonly used.
The treatment safety is observed, and the hematology of animal, hepatic and renal function are of paramount importance safety observation index.
Curative effect from treatment; Main pathological changes was glomerule infringement and tubular injury after the chronic nephritis pathological changes took place; What of urine protein become positive correlation with the glomerule lesion degree, urine N-acyl-β-D-anhydroglucose enzyme (NAG enzyme) detection by quantitative becomes positive correlation with renal tubules-ID range degree.
From the pathology index; The chronic nephritis pathological development finally causes renal fibrosis and renal failure occurs; The generation of renal failure is a tissue fibering from pathological angle representations; Tissue fibering is because the composition of component of organization such as composition mesentery substrate such as collagen, fibronectin obviously increases, and exceeds normal level.Wherein mesentery substrate index is the ratio that cellular matrix accounts for the glomerule gross area, and collagen-the 4th, one of main component in the mesentery substrate.Both numerical value increases are signs of lesion growth.More greatly then lesion degree is heavy more to that is to say mesentery substrate numerical value.
Transforming growth factor-the 1st promotes the outgrowth major cytokine of cellular matrix; Matrix metal stromatin enzyme-9 and matrix metalloprotease tissue type mortifier-the 1st, the major cytokine of regulating matrix metabolism; Wherein matrix metal stromatin enzyme-9 can degradation of cell substrate, reduces mesentery substrate; Thereby matrix metalloprotease tissue type mortifier-1 then suppresses the activity of matrix metal stromatin enzyme-9 causes the mesentery substrate degradation to reduce, and cellular matrix increases, and pathological changes increases the weight of.
Therefore observing from pathologic angle mainly is to comprise the following aspects: 1, renal tissue mesonephric glomerulus mesentery substrate index (mesentery substrate is at the glomerule proportion); 2, the expression of fibronectin; 3, the expression of transforming growth factor in the renal tissue-1, matrix metal stromatin enzyme-9 and matrix metalloprotease tissue type mortifier-1.
Express numerical value use brown or the sepia stained area as positive area.Appliance computer medical pathologies image analysis system is analyzed the result of the immunohistochemical staining of renal tissue section; Each tissue slice is gathered the positive staining area in 10 not overlapping visuals field under 200 times of optical microscopes; Calculate the AO numerical value of the pigmented section in each visual field then; Then data are gathered, use the SPSS statistical analysis software and analyze.
The present invention provides a kind of Chinese medicine composition, method for preparing and Chinese medicine preparation.This Chinese medicine composition and Chinese medicine preparation select for use four kinds of raw materials to process: the Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions.Not only practice thrift the medicine source, reduced patient's expenditure, and the test of pesticide effectiveness shows; The total effective rate of Chinese medicine composition provided by the invention and Chinese medicine preparation treatment nephritis reaches more than 96%, has the kidney tonifying spleen invigorating, the effect of blood circulation promoting and blood stasis dispelling; Can be used for the treatment of nephritis; And stable curative effect, have no side effect, be suitable for taking for a long time.The method for preparing of Chinese medicine composition provided by the invention; Adopt and collect volatile oil, vinasse, alcohol reflux liquid, medicinal residues decocting liquid respectively; Above-mentioned vinasse, alcohol reflux liquid, the merging of medicinal residues decocting liquid are got extractum after concentrating; Merge with volatile oil then, make Chinese medicine composition provided by the invention, kept the active constituents of medicine in the Chinese crude drug to greatest extent.
Used medical material or acceptable accessories all can be buied by market in Chinese medicine composition provided by the invention, method for preparing and the Chinese medicine preparation.
Below in conjunction with embodiment, further set forth the present invention:
The preparation of embodiment 1 Chinese medicine composition
Take by weighing raw material in following ratio:
Radix Astragali 5g Radix Angelicae Sinensis 120g
Radix Achyranthis Bidentatae 200g Rhizoma Chuanxiong 2g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 4h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 6 times of water gagings and decoct extraction 2 times; Each 1 hour, filter, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.1 that clear paste is measured relative density down at 80 ℃;
Step 2: it is 80 ℃ of reflux, extract, of 75% ethanol heating 3 times that the Radix Astragali, Radix Achyranthis Bidentatae add 12 times of amount percents by volume, each 1 hour, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.1 that clear paste is measured relative density down at 80 ℃; Medicinal residues add 6 times of water gagings and extract 3 times, each 1 hour, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.1 that clear paste is measured relative density down at 80 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, promptly get.
The preparation of embodiment 2 Chinese medicine compositions
Take by weighing raw material in following ratio:
Radix Astragali 60g Radix Angelicae Sinensis 50g
Radix Achyranthis Bidentatae 300g Rhizoma Chuanxiong 20g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 5h extract volatile oil; Water liquid after the distillation is collected, and medicinal residues add 9 times of water gagings and decoct extraction 1 time, each 1.5 hours; Filter; Filtrating merges aforementioned distillation back water liquid, is condensed into clear paste under normal pressure or the decompression state, and it is 1.20 that clear paste is measured relative density down at 60 ℃;
Step 2: it is 100 ℃ of reflux, extract, of 85% ethanol heating 1 time that the Radix Astragali, Radix Achyranthis Bidentatae add 7 times of amount percents by volume, each 3 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.20 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 9 times of water gagings and extract 1 time, each 3 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.20 that clear paste is measured relative density down at 60 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, promptly get.
The preparation of embodiment 3 Chinese medicine compositions
Take by weighing raw material in following ratio:
Radix Astragali 15g Radix Angelicae Sinensis 1g
Radix Achyranthis Bidentatae 164g Rhizoma Chuanxiong 200g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 2h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 7 times of water gagings and decoct extraction 1 time; Each 1 hour, filter, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.1 that clear paste is measured relative density down at 60 ℃;
Step 2: it is 90 ℃ of reflux, extract, of 90% ethanol heating 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 10 times of amount percents by volume, each 2 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.1 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 7 times of water gagings and extract 2 times, each 2 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.1 that clear paste is measured relative density down at 60 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, promptly get.
The preparation of embodiment 4 Chinese medicine compositions
Take by weighing raw material in following ratio:
Radix Astragali 300g Radix Angelicae Sinensis 3g
Radix Achyranthis Bidentatae 2.5g Rhizoma Chuanxiong 114g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 6h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 10 times of water gagings and decoct extraction 2 times; Each 1 hour, filter, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.1 that clear paste is measured relative density down at 80 ℃;
Step 2: it is 95 ℃ of reflux, extract, of 95% ethanol heating 1 time that the Radix Astragali, Radix Achyranthis Bidentatae add 8 times of amount percents by volume, each 1 hour, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.1 that clear paste is measured relative density down at 80 ℃; Medicinal residues add 10 times of water gagings and extract 1 time, each 1 hour, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.1 that clear paste is measured relative density down at 80 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, promptly get.
The preparation of embodiment 5 Chinese medicine compositions
Take by weighing raw material in following ratio:
Radix Astragali 480g Radix Angelicae Sinensis 12g
Radix Achyranthis Bidentatae 7.5g Rhizoma Chuanxiong 5g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 3h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 8 times of water gagings and decoct extraction 1 time; Each 2 hours, filter, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.1 that clear paste is measured relative density down at 60 ℃;
Step 2: it is 85 ℃ of reflux, extract, of 75% ethanol heating 3 times that the Radix Astragali, Radix Achyranthis Bidentatae add 11 times of amount percents by volume, each 1 hour, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.1 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 8 times of water gagings and extract 3 times, each 1 hour, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.1 that clear paste is measured relative density down at 60 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, promptly get.
The preparation of embodiment 6 Chinese medicine compositions
Take by weighing raw material in following ratio:
Radix Astragali 120g Radix Angelicae Sinensis 90g
Radix Achyranthis Bidentatae 240g Rhizoma Chuanxiong 75g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 8h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 12 times of water gagings and decoct extraction 2 times; Each 2 hours, filter, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.1 that clear paste is measured relative density down at 80 ℃;
Step 2: it is 80 ℃ of reflux, extract, of 60% ethanol heating 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 9 times of amount percents by volume, each 3 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.1 that clear paste is measured relative density down at 80 ℃; Medicinal residues add 12 times of water gagings and extract 2 times, each 3 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.1 that clear paste is measured relative density down at 80 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, promptly get.
The preparation of embodiment 7 tablets
Take by weighing raw material in following ratio:
Radix Astragali 120g Radix Angelicae Sinensis 24g
Radix Achyranthis Bidentatae 60g Rhizoma Chuanxiong 40g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 4h extract volatile oil; Water liquid after the distillation is collected, and medicinal residues add 11 times of water gagings and decoct extraction 2 times, each 1 hour; Filter; Filtrating merges aforementioned distillation back water liquid, is condensed into clear paste under normal pressure or the decompression state, and it is 1.20 that clear paste is measured relative density down at 60 ℃;
Step 2: it is that 75% alcohol heating reflux extracts 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 10 times of amount percents by volume, each 2 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.20 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 11 times of water gagings and extract 2 times, each 2 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.20 that clear paste is measured relative density down at 60 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, add right amount of auxiliary materials, granulation, tabletting, coating, packing promptly get Chinese medicine composition tablet of the present invention.
The preparation of embodiment 8 capsules
Take by weighing raw material in following ratio:
Radix Astragali 40g Radix Angelicae Sinensis 8g
Radix Achyranthis Bidentatae 25g Rhizoma Chuanxiong 10g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 7h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 7 times of water gagings and decoct extraction 1 time; Decocted 2 hours, and filtered, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.15 that clear paste is measured relative density down at 60 ℃;
Step 2: it is that 70% alcohol heating reflux extracts 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 8 times of amount percents by volume, each 2 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.15 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 7 times of water gagings and extract 2 times, each 1 hour, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.15 that clear paste is measured relative density down at 60 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, vacuum drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, add right amount of auxiliary materials, granulate, encapsulated, packing, promptly get Chinese medicinal composition capsules agent of the present invention.
The preparation of embodiment 9 granules
Take by weighing raw material in following ratio:
Radix Astragali 30g Radix Angelicae Sinensis 6g
Radix Achyranthis Bidentatae 15g Rhizoma Chuanxiong 10g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 5h extract volatile oil; Water liquid after the distillation is collected, and medicinal residues add 10 times of water gagings and decoct extraction 2 times, each 1 hour; Filter; Filtrating merges aforementioned distillation back water liquid, is condensed into clear paste under normal pressure or the decompression state, and it is 1.15 that clear paste is measured relative density down at 60 ℃;
Step 2: it is that 70% alcohol heating reflux extracts 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 10 times of amount percents by volume, each 2 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.15 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 10 times of water gagings and extract 2 times, each 2 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.15 that clear paste is measured relative density down at 60 ℃;
Step 3: the clear paste of combining step 1 and step 2 gained, mixing, spray drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, add right amount of auxiliary materials, granulation, drying, granulate, packing promptly get Chinese medicinal composition granules of the present invention.
The preparation of embodiment 10 pills
Take by weighing raw material in following ratio:
Radix Astragali 15g Radix Angelicae Sinensis 3g
Radix Achyranthis Bidentatae 15g Rhizoma Chuanxiong 20g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 4h extract volatile oil; Water liquid after the distillation is collected, and medicinal residues add 12 times of water gagings and decoct extraction 2 times, each 1 hour; Filter; Filtrating merges aforementioned distillation back water liquid, is condensed into clear paste under normal pressure or the decompression state, and it is 1.15 that clear paste is measured relative density down at 70 ℃;
Step 2: it is that 70% alcohol heating reflux extracts 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 10 times of amount percents by volume, each 2 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.15 that clear paste is measured relative density down at 70 ℃; Medicinal residues add 12 times of water gagings and extract 2 times, each 2 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.15 that clear paste is measured relative density down at 70 ℃;
Step 3: combining step: 1 with the clear paste of step 2 gained, mixing, spray drying obtains extract powder;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, add right amount of auxiliary materials, pill, polishing, coating, packing promptly get Chinese medicine composition pill of the present invention.
The preparation of embodiment 11 oral liquids
Take by weighing raw material in following ratio:
Radix Astragali 600g Radix Angelicae Sinensis 120g
Radix Achyranthis Bidentatae 300g Rhizoma Chuanxiong 400g
Method for preparing:
Step 1: Radix Angelicae Sinensis, Rhizoma Chuanxiong vapor distillation 6h extract volatile oil, and the water liquid after the distillation is collected, and medicinal residues add 6 times of water gagings and decoct extraction 2 times; Each 1 hour, filter, filtrating merges aforementioned distillation back water liquid; Be condensed into clear paste under normal pressure or the decompression state, it is 1.15 that clear paste is measured relative density down at 60 ℃;
Step 2: it is that 70% alcohol heating reflux extracts 2 times that the Radix Astragali, Radix Achyranthis Bidentatae add 8 times of amount percents by volume, each 2 hours, filters; Merge ethanol extract, extracting solution is condensed into clear paste after reclaiming ethanol, and it is 1.15 that clear paste is measured relative density down at 60 ℃; Medicinal residues add 6 times of water gagings and extract 2 times, each 2 hours, filter; Filtrating normal pressure or concentrating under reduced pressure become clear paste, and it is 1.15 that clear paste is measured relative density down at 60 ℃;
Step 3: combining step: 1 with the clear paste of step 2 gained;
Step 4: the clear paste of step 3 gained is added the volatile oil of step 1 gained, add right amount of auxiliary materials, configuration, packing promptly get Chinese medicine composition oral liquid of the present invention.
The test of pesticide effectiveness of embodiment 12 Chinese medicine compositions provided by the invention or Chinese medicine preparation
The treatment group: take the Chinese medicine preparation that Chinese medicine composition that the embodiment of the invention 1 to 6 provides or embodiment 7 to 11 provide, 2 times on the one, 2 months was 1 course of treatment, did not use other treatment this sick medicine and method in the course of treatment.
Matched group: do not take the Chinese medicine preparation that Chinese medicine composition that the embodiment of the invention 1 to 6 provides or embodiment 7 to 11 provide, also do not use other treatment this sick medicine and method in the course of treatment.
Table 1 nephropathy efficacy result
Influence to urine protein: the analysis and research through urine protein quantitation find, treats between preceding two groups difference and do not have significance (P＞0.05), and the situation of albuminuria appearance is similar between two groups, explains between two groups to have comparability.4 weeks of treatment back compared with 8 weeks, and the treatment group is evident in efficacy to the urine protein therapeutical effect, and curative effect difference statistical analysis has significance (P＜0.05) between two groups of the not treatment group comparisons.
Influence to the NAG enzyme: observe through two groups of treatment situation, the treatment group is evident in efficacy to the effect of urine NAG enzyme treatment, and curative effect difference statistical analysis has significance (P＜0.05) between two groups of the not treatment group comparisons.
Influence to safety indexes such as leukocyte, glutamate pyruvate transaminase, creatinine and blood urea nitrogens: through the aforementioned each item index of statistical analysis, 4 weeks and treatment back 8 all liver function indexs (glutamate pyruvate transaminase), renal function index (serum creatinine, blood urea nitrogen) and hematology's (numeration of leukocyte) are all in normal range before the treatment, after the treatment.Explain that pharmaceutical intervention does not cause obvious harm to the safety of experimental animal model.
Can know from table 1, all animals before treatment, in the therapeutic process and treatment back liver function index (glutamate pyruvate transaminase), renal function index (serum creatinine, blood urea nitrogen) and hematology's (numeration of leukocyte) all in normal range.Explain that pharmaceutical intervention does not cause obvious harm to the safety of experimental animal model.
Can find out by The above results; Medicine of the present invention has no adverse effects for hemogram, the hepatic and renal function of test rat; Have no side effect, can obviously reduce mRNA IN ADRIAMYCIN NEPHROPATHY rat excretion quantity of urinary protein, protection test rat renal tubular function; Suppressing the hardened progress of renal tissue, is a kind of medicine of ideal treatment chronic nephritis therefore.
The pathology result of the test is seen table 2 in this test, and pathology picture such as Fig. 1 are to shown in Figure 5.
Table 2 pathology result
Influence to mesentery substrate exponential sum collagen-4: the analysis and research through the different treatment groups of mRNA IN ADRIAMYCIN NEPHROPATHY rat are found; Compare between the group after the treatment; The treatment group reduces particularly collagen-4 of mesentery substrate hypertrophy; Therapeutical effect to mRNA IN ADRIAMYCIN NEPHROPATHY kidney of rats bead is evident in efficacy, and curative effect difference statistical analysis has significance (P＜0.05) between two groups of the not treatment group comparisons.
Influence to renal tissue cytokine such as transforming growth factor-1: the analysis and research through transforming growth factor-1 are found; Relatively treatment group is evident in efficacy to transforming growth factor-1 inhibitory action after the treatment, and curative effect difference statistical analysis has significance (P＜0.05) between two groups of the not treatment group comparisons.
Influence to renal tissue matrix metabolism enzyme: evident in efficacy to matrix metalloproteinase-9 with the effect of matrix metalloprotease tissue type mortifier-1 through relatively treatment group after the statistical analysis treatment; Can regulate the regulating action of matrix metalloproteinase; Suppress the expression of matrix metalloprotease tissue type mortifier-1 in renal tissue when can raise matrix metalloproteinase-9, and curative effect difference statistical analysis has significance (P＜0.05) between two groups of the not treatment group comparisons.
Can find out by The above results; Medicine of the present invention is obvious for the renal tissue effect of test rat; Can confirm from cytokine, tissue metabolism's enzyme and the ratio of mesentery substrate glomerule; Treatment group medicine can effectively suppress the Fibrotic progress of renal tissue, is a kind of medicine of ideal treatment chronic nephritis therefore.
The clinical test of pesticide effectiveness of embodiment 13 Chinese medicine compositions provided by the invention or Chinese medicine preparation
Case is selected: from Chinese medicine, analyze the clinical disease of chronic nephritis and the characteristics that pathological changes takes place, most clinically its etiology and pathogenesis of patient should be controlled from deficiency of spleen and stomach, blood-vessel obstructive opinion.Therefore syndrome-classification standard in we " the new Chinese medicine clinical research guideline " developed and published in 2002 according to national drug and food Surveillance Authority; Selection possesses chronic nephritis patient patient 60 examples of deficiency of spleen and stomach, blood-vessel obstructive syndrome; Male's 28 examples wherein, women's 32 examples, the oldest person 64 years old; Reckling 16 years old, average 38 years old; The course of disease the shortest 5 months, the longest 12 years, average 4.3 years.From present-day medical knowledge, albuminuria is one of important indicator of chronic nephritis pathological changes weight and efficacy determination.Explain that all in normal range medicine is to the human body toxic and side effects for routine blood test, liver function (glutamate pyruvate transaminase), renal function (serum creatinine, blood urea nitrogen) before and after whole case patient treatments.Urine protein qualitative (+) before the patient treatment-(++ ++), average twenty-four-hour urine protein quantification 3.86 ± 1.22 grams.
Therapeutic Method: oral recipe drug of the present invention, 2 times on the one, 1 month course of treatment.One month was 1 course of treatment, stop using in the course of treatment other treatment this sick medicine and method.
Curative effect determinate standard:
Recovery from illness: symptoms such as edema and relevant sign disappear basically, and urine protein examination continues negative, and the twenty-four-hour urine protein quantification continues less than 0.3 gram, and renal function is normal.
Produce effects: symptoms such as edema and relevant sign disappear basically, and the urine protein examination continuous decrease is more than 50%, and renal function normally or normal basically.
Effectively: symptoms such as edema and relevant sign are clearly better, and urine protein examination continuous decrease one "+" or twenty-four-hour urine protein quantification continuous decrease are more than 25%.
Invalid: clinical manifestation and lab testing all do not have the obvious improvement or the person of increasing the weight of.
Result of the test: the result of this test lists in the table 3.
Table 3 efficacy result
| Total case load | Recovery from illness | Produce effects | Effectively | Invalid |
| 60 | 5 | 36 | 17 | 2 |
Can be found out that by The above results Chinese medicine composition provided by the invention and Chinese medicine preparation are 68.33% to the treatment obvious effective rate of chronic nephritis, effective percentage is 96.67%; Have the kidney tonifying spleen invigorating, blood circulation promoting and blood stasis dispelling, effect; Can be used for the treatment of chronic nephritis, stable curative effect has no side effect; Being suitable for taking for a long time, is a kind of medicine of ideal treatment chronic nephritis therefore.
The clinical test of pesticide effectiveness of embodiment 14 Chinese medicine compositions provided by the invention or Chinese medicine preparation
Case is selected: select in case 52 examples in outpatient service or ward male 28 examples, women 24 examples; 6～33 years old age, average 15.6 years old; The course of disease 1～10 day.(1) meets State Administration of Traditional Chinese Medicine and publish " new Chinese medicine clinical research guideline "-acute nephritis diagnostic criteria; (2) course of disease is in 10 days; (3) eliminating has severe renal functional lesion person; (4) eliminating has other kidney diseases; (5) eliminating can not be adhered to the person of taking traditional Chinese medicine.(5) the dialectical blood stasis due to renal deficiency disease characteristics that meet of patient.
Curative effect determinate standard:
Recovery from illness: symptoms such as edema and relevant sign disappear basically, and urine protein examination continues negative, and the twenty-four-hour urine protein quantification continues less than 0.3 gram, and renal function is normal.
Produce effects: symptoms such as edema and relevant sign disappear basically, and the urine protein examination continuous decrease is more than 50%, and renal function normally or normal basically.
Effectively: symptoms such as edema and relevant sign are clearly better, and urine protein examination continuous decrease one "+" or twenty-four-hour urine protein quantification continuous decrease are more than 25%.
Invalid: clinical manifestation and lab testing all do not have the obvious improvement or the person of increasing the weight of.
Efficacy result is seen table 4.
Table 4 efficacy result
| Total case load | Recovery from illness | Produce effects | Effectively | Invalid |
| 52 | 23 | 19 | 9 | 1 |
Can find out by The above results; Chinese medicine composition provided by the invention and Chinese medicine preparation are 98.1% to the treatment total effective rate of chronic nephritis; Can be used for the treatment of acute nephritis, stable curative effect has no side effect; Being suitable for taking for a long time, is a kind of medicine of ideal treatment acute nephritis equally therefore.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (10)
1. a Chinese medicine composition of treating nephritis is characterized in that, is processed by following weight portion raw material:
The Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions.
2. Chinese medicine composition according to claim 1 is characterized in that, said raw material comprises: the Radix Astragali 15～60 weight portions, Radix Angelicae Sinensis 3～12 weight portions, Radix Achyranthis Bidentatae 7.5～30 weight portions, Rhizoma Chuanxiong 5～20 weight portions.
3. a method for preparing of treating the Chinese medicine composition of nephritis is characterized in that, raw material comprises the Radix Astragali 5～600 weight portions, Radix Angelicae Sinensis 1～120 weight portion, Radix Achyranthis Bidentatae 2.5～300 weight portions, Rhizoma Chuanxiong 2～200 weight portions;
Get Radix Angelicae Sinensis, Rhizoma Chuanxiong residual liquid after vapor distillation is collected volatile oil and distilled, get first medicinal residues and add water through first decoction, filter back collection first filtrating, said first filtrates mixes the concentrated clear paste of winning in back with said distillation back residual liquid; Said first clear paste is 1.10～1.20 60～80 ℃ of following relative densities;
Get the Radix Astragali, Radix Achyranthis Bidentatae through 60～95% alcohol heating reflux, collect concentrate behind the extracting solution second clear paste; Get second medicinal residues and add water and decoct through second, filter the back collect second filtrating concentrate the 3rd clear paste; Said second clear paste or said the 3rd clear paste are 1.10～1.20 60～80 ℃ of following relative densities;
Get said first clear paste, said second clear paste mixes with said the 3rd clear paste, makes extractum;
Get said extractum and mix, promptly get with said volatile oil.
4. method for preparing according to claim 3 is characterized in that, said vapor distillation is specially vapor distillation 1～2 time, each 2～8h.
5. method for preparing according to claim 3 is characterized in that, said first decoction is specially, and in g/mL, the mass volume ratio of said first medicinal residues and water is 1: 6～12.
6. method for preparing according to claim 3 is characterized in that, said backflow is specially and refluxes each 1～3h 1～3 time.
7. method for preparing according to claim 3 is characterized in that, said second decoction is specially, and in g/mL, the mass volume ratio of said second medicinal residues and water is 1: 6～12.
8. the Chinese medicine composition that makes according to each described method for preparing of claim 3 to 7.
9. a Chinese medicine preparation of treating nephritis is characterized in that, is processed by claim 1,2 or 8 described Chinese medicine compositions and acceptable accessories.
10. Chinese medicine preparation according to claim 9 is characterized in that, it is granule, capsule, tablet, pill or oral liquid.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105169309A (en) * | 2015-08-31 | 2015-12-23 | 宋晓梅 | Traditional Chinese medicine decoction for treating nephritis and preparation method thereof |
| CN106110001A (en) * | 2016-07-12 | 2016-11-16 | 佛山杰致信息科技有限公司 | A kind of Chinese medicine composition treating nephritis and preparation method thereof |
| CN106890275A (en) * | 2017-04-07 | 2017-06-27 | 苏州大学附属第医院 | A kind of Traditional Chinese medicine compound composition of delaying chronic kidney trouble progress and its clinical practice |
| CN108403989A (en) * | 2018-05-25 | 2018-08-17 | 童世新 | A kind of auxiliary oral traditional Chinese medicine preparation of kidney failure dialysis |
| CN110585381A (en) * | 2019-09-27 | 2019-12-20 | 山东佳硒雅生物有限公司 | Medicinal and edible composition for expelling toxoplasma gondii and preparation method thereof |
| CN110801466A (en) * | 2019-09-26 | 2020-02-18 | 志海医药科技(南京)有限公司 | Traditional Chinese medicine composition, preparation method and traditional Chinese medicine preparation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101085281A (en) * | 2007-06-26 | 2007-12-12 | 北京艺信堂医药研究所 | Traditional Chinese medicine for curing chronic nephritis |
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2011
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101085281A (en) * | 2007-06-26 | 2007-12-12 | 北京艺信堂医药研究所 | Traditional Chinese medicine for curing chronic nephritis |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105169309A (en) * | 2015-08-31 | 2015-12-23 | 宋晓梅 | Traditional Chinese medicine decoction for treating nephritis and preparation method thereof |
| CN106110001A (en) * | 2016-07-12 | 2016-11-16 | 佛山杰致信息科技有限公司 | A kind of Chinese medicine composition treating nephritis and preparation method thereof |
| CN106890275A (en) * | 2017-04-07 | 2017-06-27 | 苏州大学附属第医院 | A kind of Traditional Chinese medicine compound composition of delaying chronic kidney trouble progress and its clinical practice |
| CN108403989A (en) * | 2018-05-25 | 2018-08-17 | 童世新 | A kind of auxiliary oral traditional Chinese medicine preparation of kidney failure dialysis |
| CN110801466A (en) * | 2019-09-26 | 2020-02-18 | 志海医药科技(南京)有限公司 | Traditional Chinese medicine composition, preparation method and traditional Chinese medicine preparation |
| CN110585381A (en) * | 2019-09-27 | 2019-12-20 | 山东佳硒雅生物有限公司 | Medicinal and edible composition for expelling toxoplasma gondii and preparation method thereof |
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