CN102885975A - Traditional Chinese medicine composition for treating diabetes mellitus - Google Patents
Traditional Chinese medicine composition for treating diabetes mellitus Download PDFInfo
- Publication number
- CN102885975A CN102885975A CN2012104335522A CN201210433552A CN102885975A CN 102885975 A CN102885975 A CN 102885975A CN 2012104335522 A CN2012104335522 A CN 2012104335522A CN 201210433552 A CN201210433552 A CN 201210433552A CN 102885975 A CN102885975 A CN 102885975A
- Authority
- CN
- China
- Prior art keywords
- parts
- radix
- chinese medicine
- medicine composition
- diabetes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003814 drug Substances 0.000 title claims abstract description 98
- 239000000203 mixture Substances 0.000 title claims abstract description 61
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 53
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims abstract description 10
- QQILFGKZUJYXGS-UHFFFAOYSA-N Indigo dye Chemical compound C1=CC=C2C(=O)C(C3=C(C4=CC=CC=C4N3)O)=NC2=C1 QQILFGKZUJYXGS-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 244000307697 Agrimonia eupatoria Species 0.000 claims abstract description 3
- 238000002360 preparation method Methods 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- 239000002674 ointment Substances 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 235000005903 Dioscorea Nutrition 0.000 claims description 3
- 244000281702 Dioscorea villosa Species 0.000 claims description 3
- 235000000504 Dioscorea villosa Nutrition 0.000 claims description 3
- 235000021355 Stearic acid Nutrition 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims description 3
- 235000004879 dioscorea Nutrition 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 229940075507 glyceryl monostearate Drugs 0.000 claims description 3
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 claims description 3
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 239000008117 stearic acid Substances 0.000 claims description 3
- 239000003871 white petrolatum Substances 0.000 claims description 3
- 235000016993 Agrimonia Nutrition 0.000 claims description 2
- 235000000177 Indigofera tinctoria Nutrition 0.000 claims description 2
- 241001038563 Pseudostellaria Species 0.000 claims description 2
- 229940097275 indigo Drugs 0.000 claims description 2
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 claims description 2
- 229960004274 stearic acid Drugs 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 23
- 230000008901 benefit Effects 0.000 abstract description 5
- 240000004307 Citrus medica Species 0.000 abstract description 2
- 235000002722 Dioscorea batatas Nutrition 0.000 abstract 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 abstract 1
- 240000001811 Dioscorea oppositifolia Species 0.000 abstract 1
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 abstract 1
- 241000501743 Gentiana macrophylla Species 0.000 abstract 1
- 241000112528 Ligusticum striatum Species 0.000 abstract 1
- 244000241872 Lycium chinense Species 0.000 abstract 1
- 235000015468 Lycium chinense Nutrition 0.000 abstract 1
- 235000000125 common agrimony Nutrition 0.000 abstract 1
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 1
- HYAFETHFCAUJAY-UHFFFAOYSA-N pioglitazone Chemical compound N1=CC(CC)=CC=C1CCOC(C=C1)=CC=C1CC1C(=O)NC(=O)S1 HYAFETHFCAUJAY-UHFFFAOYSA-N 0.000 description 44
- 239000008280 blood Substances 0.000 description 34
- 210000004369 blood Anatomy 0.000 description 34
- 229960005095 pioglitazone Drugs 0.000 description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 21
- 239000008103 glucose Substances 0.000 description 21
- 241000700159 Rattus Species 0.000 description 14
- 210000004185 liver Anatomy 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 229940079593 drug Drugs 0.000 description 11
- 201000010099 disease Diseases 0.000 description 10
- 210000002700 urine Anatomy 0.000 description 10
- 210000004072 lung Anatomy 0.000 description 9
- 210000000952 spleen Anatomy 0.000 description 9
- 102000001554 Hemoglobins Human genes 0.000 description 8
- 108010054147 Hemoglobins Proteins 0.000 description 8
- 208000002193 Pain Diseases 0.000 description 8
- 210000003734 kidney Anatomy 0.000 description 8
- 230000006870 function Effects 0.000 description 7
- 206010011224 Cough Diseases 0.000 description 6
- 210000001124 body fluid Anatomy 0.000 description 6
- 239000010839 body fluid Substances 0.000 description 6
- 238000003304 gavage Methods 0.000 description 6
- 102000009027 Albumins Human genes 0.000 description 5
- 108010088751 Albumins Proteins 0.000 description 5
- 235000009508 confectionery Nutrition 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 210000002784 stomach Anatomy 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 229960000583 acetic acid Drugs 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 208000001848 dysentery Diseases 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000012362 glacial acetic acid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 208000034507 Haematemesis Diseases 0.000 description 3
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 206010003549 asthenia Diseases 0.000 description 3
- 230000000994 depressogenic effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000002651 drug therapy Methods 0.000 description 3
- 208000001780 epistaxis Diseases 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 210000002216 heart Anatomy 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 3
- 206010000077 Abdominal mass Diseases 0.000 description 2
- 201000000736 Amenorrhea Diseases 0.000 description 2
- 206010001928 Amenorrhoea Diseases 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 2
- 208000002249 Diabetes Complications Diseases 0.000 description 2
- 206010012655 Diabetic complications Diseases 0.000 description 2
- 208000015220 Febrile disease Diseases 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 231100000540 amenorrhea Toxicity 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 229940127089 cytotoxic agent Drugs 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000006735 deficit Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- 208000002173 dizziness Diseases 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 201000006549 dyspepsia Diseases 0.000 description 2
- 230000008451 emotion Effects 0.000 description 2
- 229960004756 ethanol Drugs 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- 208000035861 hematochezia Diseases 0.000 description 2
- 208000006750 hematuria Diseases 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012567 medical material Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 238000010791 quenching Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 229960001052 streptozocin Drugs 0.000 description 2
- -1 sublimed preparation Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 230000035922 thirst Effects 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 230000008736 traumatic injury Effects 0.000 description 2
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 description 2
- 208000035408 type 1 diabetes mellitus 1 Diseases 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 241000427159 Achyranthes Species 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000219321 Caryophyllaceae Species 0.000 description 1
- 239000003390 Chinese drug Substances 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 208000005171 Dysmenorrhea Diseases 0.000 description 1
- 206010013935 Dysmenorrhoea Diseases 0.000 description 1
- 201000000297 Erysipelas Diseases 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010019909 Hernia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000006877 Insect Bites and Stings Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 206010022998 Irritability Diseases 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 206010025421 Macule Diseases 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- 206010027514 Metrorrhagia Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241000013557 Plantaginaceae Species 0.000 description 1
- 241001038562 Pseudostellaria heterophylla Species 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282894 Sus scrofa domesticus Species 0.000 description 1
- 206010046814 Uterine prolapse Diseases 0.000 description 1
- 206010047601 Vitamin B1 deficiency Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 206010051373 Wound haemorrhage Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 208000005946 Xerostomia Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 208000002894 beriberi Diseases 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical group OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000035568 catharsis Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 208000019902 chronic diarrheal disease Diseases 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 206010013781 dry mouth Diseases 0.000 description 1
- 206010013990 dysuria Diseases 0.000 description 1
- 230000007937 eating Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 238000009207 exercise therapy Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 208000017169 kidney disease Diseases 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000002175 menstrual effect Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000012666 negative regulation of transcription by glucose Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 210000003800 pharynx Anatomy 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 208000008128 pulmonary tuberculosis Diseases 0.000 description 1
- 230000001047 pyretic effect Effects 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 231100000019 skin ulcer Toxicity 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 230000004206 stomach function Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 210000003708 urethra Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a traditional Chinese medicine composition for treating diabetes mellitus, relating to the filed of the traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-50 parts of Chinese yam, 10-20 parts of poria, 5-20 parts of radix rehmanniae, 5-15 parts of rhizoma polygonati, 5-15 parts of radix pseudostellariae, 1-10 parts of Ligusticum wallichii Franch, 5-15 parts of Chinese wolfberry, 9-15 parts of radix achyranthis bidentatae, 9-15 parts of citron, 5-15 parts of hairyvein agrimony, 1.5-9 parts of radix ophiopogonis, 5.5-15 parts of fructus aurantii, 0-10 parts of radix paeoniae rubra, 0-7 parts of gentiana macrophylla and 0-5 parts of indigo naturalis. The traditional Chinese medicine composition provided by the invention not only has an outstanding treatment effect for diabetes mellitus, but also does not have any side effect and has the advantage of remarkably improving the living quality of patients.
Description
Technical field
The present invention relates to the Chinese medicine technical field, be specifically related to a kind of Chinese medicine composition for the treatment of diabetes.
Background technology
Diabetes are a kind of incretion metabolism diseases, are owing to the relative or absolute not enough carbohydate metabolism that causes of insulin in the body is disorderly, cause the water of sugar, protein, fat and secondary, the disease of metabolic disturbance of electrolyte.Diabetes are divided two types clinically: insulin dependent diabetes mellitus (IDDM) (being type i diabetes), non-insulin-dependent diabetes mellitus (being type ii diabetes).Wherein, the type ii diabetes rate is very high, accounts for about 90% of onset diabetes number.The harm of diabetes is mainly from complication, and its incidence rate is very high, has caused high fatality rate and high disability rate.Studies show that had the patient of 30%-40% to I haven't seen you for ages a kind of complication to occur behind the onset diabetes in 10 years.Common diabetic complication has nephropathy that diabetes cause, eyes pathological changes, nervous system lesion, cardiovascular pathological changes, fatty liver etc.
World Health Organization (WHO) announces recently, nineteen ninety-five, whole world diabetics was only about 3,000 ten thousand people, and to 1997 oneself increase to 1.35 hundred million, predict according to the authoritative sources, to reach 2.4 hundred million by 2010, by 2025, will have 3.3 hundred million people to be diagnosed as diabetes, and mortality rate is only second to malignant tumor and cardiovascular diseases, is the third-largest disease that jeopardizes human life's health.
Along with the raising of living standards of the people, the incidence rate of China's diabetics and mortality rate are also increasing severely year by year.The finding that distributes from China each department, the eighties China's diabetics sickness rate less than 1%, risen to 2.65% by 1997, and more increased rapidly with annual 0.1% speed in recent years, formed an ill colony that can not be ignored.
Diabetes are called again diabetes, and the traditional Chinese medical science is thought the many because surfeit delicious food of its origin cause of formation, eating and drinking without temperance, and congenital defect, plain body is cloudy to be lost, or gives up completely to natural impulse, and disorder of emotion, or impairment caused by overstrain excessively causes cloudy body fluid deficiency consumption scorchingly then more decreases cloudy Tianjin, the two reciprocal causation.Liver is take blood as body, take gas as usefulness, and liver controlling conveyance and dispersion, the balance the body physiological function, the irritability catharsis, then happy, QI and blood is gentle, if depressed emotion or rage impairing liver, strongly fragrant and fire-transformation, liquid is hindered in the Tianjin of then burning, and viscera function is disorderly, thereby causes diabetes.Prevent and treat the diabetes key and be to keep the steady control that reaches its complication of blood glucose.The method of control blood glucose is not simple treatment, it more or not simple Drug therapy, but according to the World Health Organization (WHO) the five-term measure of control diabetes is proposed, namely diet control, exercise therapy, Drug therapy, diabetes education, blood sugar monitoring come integrated control.Wherein, Drug therapy is the key of control blood glucose.Although the chemical sugar-lowering medicine can be controlled hyperglycemia to a certain extent, because itself toxic and side effects, the organs such as the liver of human body, kidney, the heart, brain are caused serious infringement, increase the weight of the development of complication.TCM treatment of diabetes then is particular about the whole body conditioning, and giving consideration to both the incidental and fundamental is its advantage but often take effect slow.
Make a general survey of the research direction of world's new drug, mainly be single chemicals and preparations thereof of research and development the eighties in the past, begins to develop biotechnology and natural medicinal plants after the eighties, and the drug research direction is tending towards multiformity.In conjunction with the modern Chinese medicine theory of development, from improving the angle of curative effect of medication, reduction side effect, through Chinese medicine ingredients being screened the preparation that extraction and/or compatibility make, its side effect is little, and is safe, has the advantage that chemical synthetic drug can not be compared.
Summary of the invention
Large in order to overcome existing diabetes chemotherapeutic agent toxic and side effects, the prior art that the treating diabetes effect is difficult to continue is not enough, the invention provides a kind of Chinese medicine composition for the treatment of diabetes, this Chinese medicine composition good effect, and side effect is little.
The Chinese medicine composition that the present invention treats diabetes is made by the raw material of following weight portion: Rhizoma dioscoreae 10-50 part, Poria 10-20 part, Radix Rehmanniae 5-20 part, Rhizoma Polygonati 5-15 part, Radix Pseudostellariae 5-15 part, Rhizoma Chuanxiong 1-10 part, Fructus Lycii 5-15 part, Radix Achyranthis Bidentatae 9-15 part, Fructus Citri 9-15 part, Herba Agrimoniae 5-15 part, Radix Ophiopogonis 1.5-9 part, Fructus Aurantii 5.5-15 part, Radix Paeoniae Rubra 0-10 part, Radix Gentianae Macrophyllae 0-7 part, Indigo Naturalis 0-5 part.
Further preferably, the present invention's Chinese medicine composition for the treatment of diabetes is made by the raw material of following weight portion: 30 parts of Rhizoma dioscoreaes, 15 parts of Poria, 15 parts in the Radix Rehmanniae, 10 parts of Rhizoma Polygonatis, 12 parts of Radix Pseudostellariaes, 7 parts of Rhizoma Chuanxiongs, 12 parts of Fructus Lyciis, 13 parts of Radix Achyranthis Bidentataes, 14 parts of Fructus Citris, 10 parts of Herba Agrimoniaes, 5 parts of Radix Ophiopogonis, 11 parts of Fructus Aurantiis, 7 parts of Radix Paeoniae Rubra, 5 parts of Radix Gentianae Macrophyllae, 2 parts of Indigo Naturaliss.
Rhizoma dioscoreae refers to the Rhizoma Dioscoreaes that is produced from ground such as Anhui, modern Jiangsu, and the Rhizoma dioscoreae stem is with aubergine usually, cures mainly insufficiency of the spleen lack of appetite, incessant chronic diarrhea, and the spleen reinforcing nourishing the stomach is arranged, the lung benefiting that promotes the production of body fluid, the effect of the kidney invigorating arresting seminal emission.
Poria is commonly called as Poria, and sweet in the mouth, light, property are flat, enter the heart, lung, spleen channel; Be used as medicine have promoting diuresis to eliminate damp pathogen, the function of strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming; Modern medicine study: Poria energy enhancing human body immunity function, pachyman has obvious antitumor and protects the liver dirty effect.
The Radix Rehmanniae also is Radix Rehmanniae, the Scrophulariaceae herbaceos perennial; Sweet in the mouth, hardship, cold nature, GUIXIN, liver, kidney channel; The matter profit is fallen and is let out, and the nourishing YIN to relieve dryness effect of promoting the production of body fluid is arranged.
Rhizoma Polygonati is used as medicine with rhizome, has boosting qi and nourishing yin, spleen invigorating, lung moistening, kidney benefiting functions; Be used for the treatment of weakness of the spleen and stomach, fatigue and asthenia, xerostomia lack of appetite, deficiency of the lung cough caused by dryness, asthenia of essence and blood, the interior-heat disease such as quench one's thirst.
Radix Pseudostellariae has another name called Radix Ginseng, virgin ginseng; Tuber for Caryophyllaceae herbaceos perennial pseudostellaria heterophylla; Invigorating qi and benefiting blood arranged, promote the production of body fluid, the effect of tonifying the spleen and stomach; Be suitable for children's's summer heat of a specified duration do not move back, the disease of the weaknesses such as diet is depressed, the deficiency of the lung, cough, cardiopalmus and children's after being ill weak unable, spontaneous perspiration, night sweat, mouthful equality disease.
Rhizoma Chuanxiong, Xin Wenxiang is dry, walks and does not keep, can go loose, the up top, mountain peak that reaches; Enter again blood system, the descending sea of blood that reaches; The blood circulation promoting and blood stasis dispelling effect is extensive, the suitable various diseases of stagnation of blood stasis; Wind-expelling pain-stopping, effectiveness is excellent, can control the diseases such as wind syndrome of head headache, rheumatic arthralgia.
Fructus Lycii, sweet in the mouth, property is flat returns Liver Channel, kidney channel, lung meridian; Cure mainly: caused by liver and kidney deficiency, have a dizzy spell, the disease such as visual unclear, soreness of the waist and knees, impotence and seminal emission, cough due to consumptive disease, the taking fluids profusely of quenching one's thirst.
Radix Achyranthis Bidentatae is the plant of Amaranthaceae Achyranthes, and its property bitter but sweet flavor acid is flat, has promoting blood circulation to restore menstrual flow, invigorating the liver and kidney, bone and muscle strengthening, inducing diuresis for treating stranguria syndrome, draw the descending effect of blood (fire), be usually used in treating the gynaecopathia such as amenorrhea, dysmenorrhea, menoxenia, puerperal abdonimal pain of stagnation of blood stasis, traumatic injury, the soreness of waist and knee joint of suffering from a deficiency of the kidney, weakness of the lower extremities, hematuria, dysuria, Urethra astringent pain and intimately go up the cards such as headache that inflammation causes, dizzy, haematemesis, epistaxis.
Fructus Citri has another name called citron, is the Rutaceae citrus plant, and Fructus Citri acrid in the mouth, little hardship, acid are warm in nature; Return liver, lung, spleen channel; The fragrant row of gas is loose, can rise and can fall; Have depressed liver-energy dispersing and QI regulating, the chest stuffiness relieving reduces phlegm, dehumidifying and in effect, cure mainly distending pain in the chest and hypochondrium, cough with copious phlegm, gastral cavity abdomen painful abdominal mass pain, dyspepsia vomiting, edema beriberi.
Herba Agrimoniae, property is bitter, puckery, and is flat; GUIXIN, Liver Channel cure mainly astringing to arrest bleeding, dysentery relieving, parasite killing; Be widely used in various hemorrhage cards, for example: haematemesis, hematuria, have blood in stool, metrorrhagia, spitting of blood, epistaxis, dysentery, impairment caused by overstrain is taken off power, carbuncle, traumatic injury, the cards such as wound hemorrhage.
Have another name called Radix Ophiopogonis Radix Ophiopogonis, is Liliaceae Ophiopogon plant; Be used as medicine with tuber Radix Ophiopogonis; Nature,taste and action: sweet, little hardship, cold, Yin-nourishing and body fluid promoting, nourishing the lung to arrest cough, relieving restlessness clears away heart-fire; Cure mainly consumption of body fluid caused by febrile disease, vexed, thirsty, dry pharynx lung-heat, cough, pulmonary tuberculosis.
Fructus Aurantii, nature and flavor are bitter, acid, cold nature; Gui Jing: lung, spleen, liver, stomach, large intestine channel; Function cures mainly: the septum pectorale feeling of fullness, and distending pain over the hypochondrium, accumulation of food in the stomach and intes tine due to indigestion, distension and fullness in the abdomen, heavy behind the dysentery, proctoptosis, uterine prolapse.
Radix Paeoniae Rubra, function: clots absorbing, pain relieving, removing heat from blood, detumescence; Cure mainly: control that the stasis of blood stagnates that amenorrhea, hernia abdominal mass gather, stomachache, hypochondriac pain, epistaxis, dysentery, discharging fresh blood stool, conjunctival congestion, carbuncle, injury from falling down.
Radix Gentianae Macrophyllae has Eradicates rheumatism, Shujin network, the effect of clearind deficient heat; Cold in nature, salty in the mouth, the function clearing away heat-fire, removing pathogenic heat from blood and toxic substance from the body cures mainly pyretic toxicity and sends out the diseases such as speckle, haematemesis.
Indigo Naturalis, heat clearing away, removing heat from blood, detoxifcation; Control the epidemic febrile disease intenseness of heat, macule is spitted blood, is spat blood, pediatric epilepsy scared, and skin ulcer is swollen, erysipelas, snake bite and insect sting.
In order to express better Chinese medicine composition of the present invention, Chinese medicine composition of the present invention can be prepared into dosage form commonly used clinically.Such as, the preparations such as powderous preparations, powder, pill, sublimed preparation, unguentum, granule, oral liquid, syrup, tablet, capsule.Preferably, Chinese medicine composition of the present invention is prepared into powder, unguentum or water preparation according to conventional preparation technology.
The present invention also provides a kind of preparation method for preparing above-mentioned Chinese medicine composition unguentum, and it mainly comprises following step: at first, get Rhizoma dioscoreae, Poria, the Radix Rehmanniae, Rhizoma Polygonati, Radix Pseudostellariae, Rhizoma Chuanxiong, Fructus Lycii, Radix Achyranthis Bidentatae, Fructus Citri, Herba Agrimoniae, Radix Ophiopogonis, Fructus Aurantii, Radix Paeoniae Rubra, Radix Gentianae Macrophyllae and Indigo Naturalis are broken into coarse powder, 4-9 according to the coarse powder gross weight doubly adds the alcoholic solution that volumetric concentration is 40%-95%, reflux, extract, three times, return time are 2-5h, filter, filtrate recycling ethanol, cold filtration washes with water, namely gets Chinese medical concrete after the drying; Next adds white vaseline, glyceryl monostearate, stearic acid and distilled water and makes unguentum.Those skilled in the art can be at the technical clinically conventional Chinese medicine pharmaceutical dosage form for preparing of this preparation method, such as tablet, capsule etc.
The present invention also asks for protection the purposes of above-mentioned Chinese medicine composition in preparation treatment diabetes medicament.Drug effect embodiment 15 of the present invention shows, there is significant synergism in Chinese medicine composition Chinese medicine composition of the present invention in treating diabetes, it is compared with the positive control drug pioglitazone, not only can significantly reduce fasting glucose and 2h-plasma glucose, and can be remarkable.This shows that Chinese medicine composition of the present invention not only can significantly improve the symptom of diabetes in treating diabetes, can also delay the development of diabetes, the generation of complication.
In a word, the present invention compared with prior art has following advantage:
1) compares with the positive control drug pioglitazone, pharmaceutical composition Chinese medicine composition of the present invention is not only more remarkable aspect reduction fasting glucose and 2h-plasma glucose, and also significantly be better than positive drug aspect reduction glycolated hemoglobin and the urine trace albumin, this shows that the present composition has significant synergism in treating diabetes, not only can significantly improve the symptom of diabetes, can also delay the development of diabetes, the generation of complication.
2) compare with the chemotherapeutic agent of current treatment diabetes, Chinese medicine composition of the present invention is natural pure Chinese medicinal preparation, untoward reaction and side effect significantly reduce, and Chinese medicine composition effect of the present invention is comprehensive, medication effect is better, significantly improve the compliance of diabetics, and improved Quality of Life.
3) existing Remedies for diabetes diabetes initial stage therapeutic effect still can, but along with obvious drug resistance problem all appears in the prolongation for the treatment of time, the therapeutic effect of diabetes descends.Contain the multi-medicament component in the Chinese medicine composition of the present invention, action target spot is numerous, has effectively solved the Commpensation And Adaptation of Remedies for diabetes, and its therapeutic effect to diabetes does not descend because of the treatment time prolongation.
The specific embodiment
Below further describe the present invention by specific embodiment, the present invention is not limited only to following examples.Within the scope of the invention or within not breaking away from content of the present invention, spirit and scope, to change, combination or the replacement that the present invention carries out, be apparent for a person skilled in the art, and be included within the scope of the present invention.
First's Chinese medicinal composition preparation of the present invention, preparation and using method thereof
Embodiment 1-6 Chinese medicine composition composition powders
Table 1 Chinese medicine composition composition powders prescription of the present invention
Embodiment 1 preparation method: get each medical material in the compositions, grind to form powdery according to common process, cross 100 mesh sieves, little fire drying is for subsequent use, namely gets powder.During use, with directly taking after mixing it with water behind the water dissolution.Embodiment 2-6 preparation method is with embodiment 1.
Embodiment 7-12 Chinese medicine composition water preparation
Table 2 Chinese medicine composition water preparation prescription of the present invention
Embodiment 7 preparation methoies: get each medical material in the prescription, according to conventional decocting for Chinese herbal medicine technological operation, filtering medicinal residues and get final product.This water preparation (or claiming decoction) when being used for treating diabetes, take 2-3 time by every day.Embodiment 8-12 preparation method and using method are with embodiment 7.
Embodiment 13-18 Chinese medicine composition unguentum of the present invention
Table 3 Chinese medicine composition unguentum prescription of the present invention
Embodiment 13 preparation technologies: at first, each traditional Chinese medicine powder of getting recipe quantity is broken into coarse powder, adding volumetric concentrations according to 5 times of the coarse powder gross weight is 80% alcoholic solution, reflux, extract, three times, the each 3h of return time filters, filtrate recycling ethanol, cold filtration washes with water, namely gets Chinese medical concrete after the drying; Next adds 100g white vaseline, 20g glyceryl monostearate, 120g stearic acid and an amount of distilled water and makes unguentum.
Embodiment 14-18 preparation technology is with embodiment 13.
Second portion Chinese medicine composition pharmacodynamic study of the present invention
Embodiment 15: Chinese medicine composition of the present invention causes the therapeutical effect of diabetes rat model to streptozotocin
1, experimental diabetic animal models preparation
Body weight 160-180gWistar rat, male and female half and half, feed first take high-sugar-fat-diet (albumen select matter 5%, carbohydrate 60% wherein sucrose as 30%, fat 32% wherein refines Adeps Sus domestica as 30%) after 4 weeks, rat fasting 18h.Lumbar injection 0.6% streptozotocin (STZ) 30mg/kg, STZ is dissolved in pH4.0, and in the 0.1mol/L citric acid-sodium citrate buffer, each dose is finished in 10min.Blank group rats by intraperitoneal injection equal-volume citric acid-sodium citrate buffer, the normal raising.Docked afterwards in 5 days and get blood survey whole blood sugar, the success take blood glucose value level＞10.0mmol/L person as modeling.
2, experiment grouping and administration
Modeling success rat is divided into model control group at random according to blood sugar level, pioglitazone group, Chinese medicine composition A group, Chinese medicine composition B group, Chinese medicine composition C group, totally 6 groups of Chinese medicine composition D groups, 10 every group.Each group gives respectively following medicine:
Model control group: gavage gives the equal-volume normal saline;
The pioglitazone group: gavage gives pioglitazone 2.5mg/kgd;
Chinese medicine A group: the crude drug amount that gavage gives embodiment 1 preparation is the Chinese medicine composition composition powders of 1g/kgd;
Chinese medicine B group: the crude drug amount that gavage gives embodiment 7 preparations is the Chinese medicine composition water preparation of 3g/kgd;
Chinese medicine C group: the crude drug amount that gavage gives embodiment 13 preparations is the Chinese medicine composition unguentum of 3g/kgd;
Chinese medicine D group: the crude drug amount that gavage gives embodiment 18 preparations is 10g/kgd Chinese medicine composition unguentum
Above-mentioned administration group is administered once every day, continuous 10 weeks.Fasting glucose, the rear 2h blood glucose of feed, glycolated hemoglobin (HbAlc) and urine trace albumin are measured in blood sampling.
3, index determining
3.1 the mensuration of blood glucose: after modeling success rat random packet, according to dosage gastric infusion in continuous 10 weeks, is normally raised.All rats are respectively at getting weekly tail vein detection fasting glucose (FBG), 2h blood glucose (PBG) after the feed before treating and after the treatment in 4 weeks.The blood sample that takes out is put into protein precipitant, after room temperature is placed 7min, centrifugal 5min(3000r/min), get supernatant, survey whole blood sugar with glucose oxidase method.
3.2 the mensuration of glycolated hemoglobin (HbAlc): (grouping, the same blood sugar detection of administration) fasting 12h after the last administration, etherization, eye socket is got blood, presses description mensuration HbAlc in the test kit.
3.3 the mensuration of microalbumin in the urine:
Reagent: a, 10%(v/v) glacial acetic acid solution (PH2.8).
B, 0.303mol/L glycine-glacial acetic acid buffer (PH3.0): take by weighing the 22.72g glycine, be diluted to 1000ml with 10% glacial acetic acid solution, add NaN
3100mg, the room temperature sealing can be stablized 1 year.
C, bromophenol blue (1.924mmol/L) stock solution: accurately take by weighing 257,36mgBPB, molten to 200ml with dehydrated alcohol, 4 ℃ of refrigerators can be stablized 1 year.
D, bromophenol blue (0.231mmol/L) developer: get the 60mlBPB stock solution, add 2.5mlTriton X-100, be diluted to 500ml with glycine-glacial acetic acid buffer, the room temperature sealing can be preserved 1 year.
The collection of specimen and detection: in the 1st, 3,7 and 10 weeks rat is put in the metabolic cage respectively and raises, collect 12 hours overnight urine, accurate recording urine amount.Get 4ml, after sodium azide was processed, centrifugal (2000r/min) 10min got supernatant and puts-20 ℃ of Refrigerator store urinaryalbumin to be measured.Get the albumin standards 400 of respective concentration in the cup of correspondence, respectively add 200 developers, mixing (preventing bubble) is used ultraviolet spectrophotometer, measures absorbance A under 600nm.
4, experimental result and discussion
4.1 the impact of Chinese medicine composition blood glucose in diabetic rats of the present invention
Table 1 Chinese medicine composition of the present invention is on the impact of diabetes rat fasting glucose and the rear 2h blood glucose of feed
*Compare with model group, P＜0.05,
*Compare P＜0.01 with model group;
#Compare with the pioglitazone group, P＜0.05,
##Compare P＜0.01 with the pioglitazone group.
Above-mentioned experimental result shows, Chinese medicine composition is compared with pioglitazone in that to reduce aspect fasting glucose and the 2h-plasma glucose effect better.Be embodied in:
1) no matter be fasting glucose or 2h-plasma glucose, pioglitazone group and Chinese medicine A, B, C, D group have significant difference with model group, and Chinese drug-treated group and model group relatively have utmost point significant difference;
2) compare with the pioglitazone group, each group of Chinese medicine is having utmost point significant difference aspect the reduction fasting glucose;
3) compare with the pioglitazone group, Chinese medicine A, B, C group are having significant difference aspect the reduction 2h-plasma glucose, and Chinese medicine D group has been compared significant difference with the pioglitazone group.
4.2 Chinese medicine composition of the present invention is on the impact of diabetes rat glycolated hemoglobin
Table 2 Chinese medicine composition of the present invention is on the impact of diabetes rat HbAlc
*Compare with model group, P＜0.05,
*Compare P＜0.01 with model group;
#Compare with the pioglitazone group, P＜0.05,
##Compare P＜0.01 with the pioglitazone group.
Experimental result shows, Chinese medicine composition is compared with the pioglitazone group in that to reduce aspect the glycolated hemoglobin effect better, has remarkable synergism between each composition of Chinese medicine composition.Be embodied in:
1) compare with model control group, the glycolated hemoglobin no difference of science of statistics after the treatment of pioglitazone group, the saccharification hemoglobin content of each group of Chinese medicine all has significant difference;
2) compare with model control group, the saccharification hemoglobin content of each group of Chinese medicine all has significant difference, and wherein Chinese medicine C group and Chinese medicine D group have utmost point significant difference.
4.3 the impact of Chinese medicine composition diabetes rat microdose urine protein of the present invention
Table 3 Chinese medicine composition of the present invention is on the impact of diabetes rat microdose urine protein
| Group | n | Absorbance A (600nm) |
| Model control group | 10 | 0.6841±0.057 |
| The pioglitazone group | 10 | 0.5496±0.102 * |
| Chinese medicine A group | 10 | 0.5144±0.301 * |
| Chinese medicine B group | 10 | 0.5037±0.124 **# |
| Chinese medicine C group | 10 | 0.4867±0.187 **# |
| Chinese medicine D group | 10 | 0.4201±0.155 **## |
*Compare with model group, P＜0.05,
*Compare P＜0.01 with model group;
#Compare with the pioglitazone group, P＜0.05,
##Compare P＜0.01 with the pioglitazone group.
Experimental result shows, Chinese medicine composition has good synergism aspect the diabetes rat microdose urine protein affecting, and its therapeutic effect significantly is better than positive control drug pioglitazone group.This shows that Chinese medicine composition is delaying to have outstanding treatment advantage aspect diabetes PD, the minimizing diabetic complication.Be in particular in:
1) compare with model group, the urine trace albumin content of each group of pioglitazone group and Chinese medicine all has significant difference, and wherein each group of Chinese medicine has utmost point significant difference;
2) compare with the pioglitazone group, the urine trace albumin content of Chinese medicine B, C, D group has significant difference.
Claims (4)
1. Chinese medicine composition for the treatment of diabetes is characterized in that being made by the raw material of following weight portion:
Rhizoma dioscoreae 10-50 part, Poria 10-20 part, Radix Rehmanniae 5-20 part, Rhizoma Polygonati 5-15 part, Radix Pseudostellariae 5-15 part, Rhizoma Chuanxiong 1-10 part, Fructus Lycii 5-15 part, Radix Achyranthis Bidentatae 9-15 part, Fructus Citri 9-15 part, Herba Agrimoniae 5-15 part, Radix Ophiopogonis 1.5-9 part, Fructus Aurantii 5.5-15 part, Radix Paeoniae Rubra 0-10 part, Radix Gentianae Macrophyllae 0-7 part, Indigo Naturalis 0-5 part.
2. the Chinese medicine composition for the treatment of diabetes as claimed in claim 1 is characterized in that being made by the raw material of following weight portion: 30 parts of Rhizoma dioscoreaes, 15 parts of Poria, 15 parts in the Radix Rehmanniae, 10 parts of Rhizoma Polygonatis, 12 parts of Radix Pseudostellariaes, 7 parts of Rhizoma Chuanxiongs, 12 parts of Fructus Lyciis, 13 parts of Radix Achyranthis Bidentataes, 14 parts of Fructus Citris, 10 parts of Herba Agrimoniaes, 5 parts of Radix Ophiopogonis, 11 parts of Fructus Aurantiis, 7 parts of Radix Paeoniae Rubra, 5 parts of Radix Gentianae Macrophyllae, 2 parts of Indigo Naturaliss.
3. the Chinese medicine composition for the treatment of diabetes as claimed in claim 1 or 2, it is characterized in that: described Chinese medicine composition is powder, unguentum or water preparation.
4. the preparation method of the Chinese medicine composition unguentum for the treatment of diabetes as claimed in claim 3, it is characterized in that may further comprise the steps: at first, get Rhizoma dioscoreae, Poria, the Radix Rehmanniae, Rhizoma Polygonati, Radix Pseudostellariae, Rhizoma Chuanxiong, Fructus Lycii, Radix Achyranthis Bidentatae, Fructus Citri, Herba Agrimoniae, Radix Ophiopogonis, Fructus Aurantii, Radix Paeoniae Rubra, Radix Gentianae Macrophyllae and Indigo Naturalis and be broken into coarse powder, 4-9 according to the coarse powder gross weight doubly adds the alcoholic solution that volumetric concentration is 40%-95%, reflux, extract, three times, return time is 2-5h, filter, filtrate recycling ethanol, cold filtration, wash with water, namely get Chinese medical concrete after the drying; Secondly, add white vaseline, glyceryl monostearate, stearic acid and distilled water and make unguentum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210433552.2A CN102885975B (en) | 2012-11-05 | 2012-11-05 | Traditional Chinese medicine composition for treating diabetes mellitus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210433552.2A CN102885975B (en) | 2012-11-05 | 2012-11-05 | Traditional Chinese medicine composition for treating diabetes mellitus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102885975A true CN102885975A (en) | 2013-01-23 |
| CN102885975B CN102885975B (en) | 2014-01-08 |
Family
ID=47529792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210433552.2A Active CN102885975B (en) | 2012-11-05 | 2012-11-05 | Traditional Chinese medicine composition for treating diabetes mellitus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102885975B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103223111A (en) * | 2013-05-18 | 2013-07-31 | 崔新明 | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof |
| CN103432367A (en) * | 2013-08-17 | 2013-12-11 | 广州市云桥生物科技有限公司 | Traditional Chinese medicine effective part composition for treating diabetes |
| CN103989940A (en) * | 2014-06-09 | 2014-08-20 | 杨献华 | Traditional Chinese medicine composition for treating diabetes mellitus |
| CN104606436A (en) * | 2015-01-08 | 2015-05-13 | 季一艮 | Traditional Chinese medicine composition for treating senility diabetes mellitus |
| CN104771709A (en) * | 2015-04-27 | 2015-07-15 | 青岛辰达生物科技有限公司 | Traditional Chinese medicine composition for treating diabetes with renal injury and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090193A (en) * | 1993-05-03 | 1994-08-03 | 孙志远 | The medicine and the production method of treatment diabetes |
| CN1443544A (en) * | 2002-03-07 | 2003-09-24 | 张世均 | Chinese medicine Jiangtang Tongmailing for curing diabetes and preventing and curing complicating diseases and its preparation method |
| CN101485834A (en) * | 2009-02-19 | 2009-07-22 | 张家寅 | Chinese medicine for treating diabetes |
| CN101720919A (en) * | 2008-10-22 | 2010-06-09 | 沈正芳 | Medlar mulberry hypoglycemic tea |
-
2012
- 2012-11-05 CN CN201210433552.2A patent/CN102885975B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1090193A (en) * | 1993-05-03 | 1994-08-03 | 孙志远 | The medicine and the production method of treatment diabetes |
| CN1443544A (en) * | 2002-03-07 | 2003-09-24 | 张世均 | Chinese medicine Jiangtang Tongmailing for curing diabetes and preventing and curing complicating diseases and its preparation method |
| CN101720919A (en) * | 2008-10-22 | 2010-06-09 | 沈正芳 | Medlar mulberry hypoglycemic tea |
| CN101485834A (en) * | 2009-02-19 | 2009-07-22 | 张家寅 | Chinese medicine for treating diabetes |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103223111A (en) * | 2013-05-18 | 2013-07-31 | 崔新明 | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof |
| CN103223111B (en) * | 2013-05-18 | 2014-07-30 | 崔新明 | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof |
| CN103432367A (en) * | 2013-08-17 | 2013-12-11 | 广州市云桥生物科技有限公司 | Traditional Chinese medicine effective part composition for treating diabetes |
| CN103989940A (en) * | 2014-06-09 | 2014-08-20 | 杨献华 | Traditional Chinese medicine composition for treating diabetes mellitus |
| CN104606436A (en) * | 2015-01-08 | 2015-05-13 | 季一艮 | Traditional Chinese medicine composition for treating senility diabetes mellitus |
| CN104771709A (en) * | 2015-04-27 | 2015-07-15 | 青岛辰达生物科技有限公司 | Traditional Chinese medicine composition for treating diabetes with renal injury and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102885975B (en) | 2014-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102048933B (en) | Chinese medicinal composition for treating infantile diarrhea and preparation method thereof | |
| CN101797326B (en) | Chinese materia medica preparation for treating borborygmus diarrhoea and preparation method thereof | |
| CN102205107B (en) | Chinese medicinal preparation for treating duodenal ulcer and preparation method thereof | |
| CN103520572B (en) | A kind of Chinese medicine composition being used for the treatment of atopic dermatitis and preparation method thereof | |
| CN103223111B (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof | |
| CN102784363A (en) | Traditional Chinese medicine composition for treating pancreatic cancer and preparation method thereof | |
| CN102885975B (en) | Traditional Chinese medicine composition for treating diabetes mellitus | |
| CN103690784B (en) | The Chinese medicine composition for the treatment of Complicating with Hypentersion nephropathy and preparation method | |
| CN103041256B (en) | Chinese herb preparation for curing recurrent respiratory tract infection of children | |
| CN104083640B (en) | Traditional Chinese medicinal composition for treating diabetes mellitus and preparation method thereof | |
| CN105497816A (en) | Traditional Chinese medicine preparation for treating atherosclerosis and preparation method thereof | |
| CN103041257B (en) | Chinese materia medica preparation for curing high fever and respiratory tract infection of children | |
| CN106994141B (en) | Traditional Chinese medicine composition for treating constipation and preparation method thereof | |
| CN103705860B (en) | A kind of Chinese medicine composition for the treatment of infant jaundice and preparation method thereof | |
| CN105343593A (en) | Spica prunellae health oral liquid capable of decreasing blood pressure and blood lipid as well as preparation method of spica prunellae health oral liquid | |
| CN103041255B (en) | Chinese materia medica preparation for curing swollen sore throat and respiratory tract infection of children | |
| CN106214787A (en) | A kind of Chinese medicine composition treating diabetes and preparation method thereof and purposes | |
| CN103989940A (en) | Traditional Chinese medicine composition for treating diabetes mellitus | |
| CN104689151A (en) | Traditional Chinese medicine composition for treating diabetes and use of composition | |
| CN104352748A (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof | |
| CN104436022A (en) | Hemsleya-macrosperma-containing traditional Chinese medicine composition for treating diabetes mellitus | |
| CN103007145A (en) | Traditional Chinese medicine composition for treating children rotavirus enteritis and preparation method of composition | |
| CN103191297B (en) | Traditional Chinese medicine composition for treating male infertility and preparation method thereof | |
| CN103041258B (en) | Chinese materia medica preparation for curing respiratory tract infection with poor appetite of children | |
| CN106902250A (en) | A kind of compound medicine of traditional Chinese and western medicine therapeutic alliance diabetes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: NANTONG HAOYOUXIAN AQUATIC FOOD CO., LTD. Free format text: FORMER OWNER: YANG XIANHUA Effective date: 20140730 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 276017 LINYI, SHANDONG PROVINCE TO: 226200 NANTONG, JIANGSU PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20140730 Address after: 226200 Qidong City, Jiangsu Province, Lu Lu, the Marine Economic Development Zone, four Patentee after: Nantong good and fresh aquatic food Co., Ltd. Address before: 276017, Shandong Linyi hi tech Industrial Development Zone, West building, the third floor of the West Di science and Technology Information Service Center Patentee before: Yang Xianhua |