CN102869773A - 无色肽酶用于室温下裂解的用途 - Google Patents

无色肽酶用于室温下裂解的用途 Download PDF

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Publication number
CN102869773A
CN102869773A CN2011800203890A CN201180020389A CN102869773A CN 102869773 A CN102869773 A CN 102869773A CN 2011800203890 A CN2011800203890 A CN 2011800203890A CN 201180020389 A CN201180020389 A CN 201180020389A CN 102869773 A CN102869773 A CN 102869773A
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Prior art keywords
biological sample
nucleic acid
sample
cracking
achromopeptidase
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CN2011800203890A
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English (en)
Chinese (zh)
Inventor
D·海利高斯
L·M·科勒
S·拉马丹
J·寇迪
T·J·赫雷尔
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Becton Dickinson and Co
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Becton Dickinson and Co
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Publication of CN102869773A publication Critical patent/CN102869773A/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800203890A 2010-03-16 2011-03-15 无色肽酶用于室温下裂解的用途 Pending CN102869773A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31431810P 2010-03-16 2010-03-16
US61/314,318 2010-03-16
PCT/US2011/028494 WO2011115975A2 (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature

Publications (1)

Publication Number Publication Date
CN102869773A true CN102869773A (zh) 2013-01-09

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CN2011800203890A Pending CN102869773A (zh) 2010-03-16 2011-03-15 无色肽酶用于室温下裂解的用途

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US (1) US20110275090A1 (enExample)
EP (1) EP2547767A4 (enExample)
JP (1) JP2013521804A (enExample)
CN (1) CN102869773A (enExample)
AU (1) AU2011227452B2 (enExample)
BR (1) BR112012023875A2 (enExample)
CA (1) CA2793144A1 (enExample)
WO (1) WO2011115975A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10654021B2 (en) 2016-11-14 2020-05-19 Airbus Operations Gmbh Autoclave and method for welding thermoplastic composite parts
CN118834934A (zh) * 2024-08-19 2024-10-25 希莱乐检(郑州)生物科技有限公司 用于结核分枝杆菌核酸释放的裂解液及其应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8597884B2 (en) 2011-03-09 2013-12-03 Becton, Dickinson And Company Process controls for molecular assay
US9528987B2 (en) 2011-06-23 2016-12-27 University Of Washington Reagent patterning in capillarity-based analyzers and associated systems and methods
WO2013117968A1 (en) 2012-02-10 2013-08-15 Reametrix Inc. Methods for lysing bacteria from sample and isolating cellular components therefrom, and kits therefor
EP2948249A1 (en) 2013-01-22 2015-12-02 University of Washington through its Center for Commercialization Sequential delivery of fluid volumes and associated devices, systems and methods
CN111278844B (zh) * 2017-09-13 2023-12-19 贝克顿·迪金森公司 用于使用氧化铁颗粒提取核酸的方法和组合物
WO2021217173A1 (en) * 2020-04-21 2021-10-28 Siemens Healthcare Diagnostics Inc. Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof

Citations (3)

* Cited by examiner, † Cited by third party
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US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
US5508164A (en) * 1990-10-29 1996-04-16 Dekalb Genetics Corporation Isolation of biological materials using magnetic particles
US20040002594A1 (en) * 2002-06-27 2004-01-01 John Krupey Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

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GB8414273D0 (en) * 1984-06-05 1984-07-11 Oxoid Ltd Identifying streptococcal grouping
JP2589729B2 (ja) * 1988-01-30 1997-03-12 極東製薬工業株式会社 染色体dnaを用いた細菌の同定方法および該同定用キット
US5973138A (en) * 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
JP3172917B2 (ja) * 1999-07-23 2001-06-04 北海道 細菌検出方法
US7595176B2 (en) * 2003-05-30 2009-09-29 Georgia Tech Research Corporation Methods and reagents for quantitative analysis of Dehalococcoides species
WO2005064016A1 (ja) * 2003-12-26 2005-07-14 Prima Meat Packers, Ltd. 微生物の多重検出方法
US20060057613A1 (en) * 2004-07-26 2006-03-16 Nanosphere, Inc. Method for distinguishing methicillin resistant S. aureus from methicillin sensitive S. aureus in a mixed culture
AU2006271104B2 (en) * 2005-07-21 2011-01-27 Morinaga Milk Industry Co., Ltd. Method for detection of microorganism and kit for detection of microorganism
JP2010510524A (ja) * 2006-11-22 2010-04-02 スリーエム イノベイティブ プロパティズ カンパニー 試料を調製及び分析するシステム並びに方法
JP2008148570A (ja) * 2006-12-14 2008-07-03 Hitachi Ltd 微生物検出システム
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090274577A1 (en) * 2008-05-05 2009-11-05 B-K Medical Aps Sterilization by treatment with reductant and oxidant

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5508164A (en) * 1990-10-29 1996-04-16 Dekalb Genetics Corporation Isolation of biological materials using magnetic particles
US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
US20040002594A1 (en) * 2002-06-27 2004-01-01 John Krupey Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10654021B2 (en) 2016-11-14 2020-05-19 Airbus Operations Gmbh Autoclave and method for welding thermoplastic composite parts
CN118834934A (zh) * 2024-08-19 2024-10-25 希莱乐检(郑州)生物科技有限公司 用于结核分枝杆菌核酸释放的裂解液及其应用
CN118834934B (zh) * 2024-08-19 2025-05-16 希莱乐检(郑州)生物科技有限公司 用于结核分枝杆菌核酸释放的裂解液及其应用

Also Published As

Publication number Publication date
CA2793144A1 (en) 2011-09-22
JP2013521804A (ja) 2013-06-13
US20110275090A1 (en) 2011-11-10
AU2011227452A1 (en) 2012-10-04
WO2011115975A3 (en) 2012-01-19
WO2011115975A2 (en) 2011-09-22
EP2547767A4 (en) 2013-12-25
AU2011227452B2 (en) 2015-04-09
BR112012023875A2 (pt) 2015-09-22
EP2547767A2 (en) 2013-01-23

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Application publication date: 20130109