BR112012023875A2 - uso de acromopeptidase para lise em temperatura ambiente - Google Patents

uso de acromopeptidase para lise em temperatura ambiente

Info

Publication number
BR112012023875A2
BR112012023875A2 BR112012023875A BR112012023875A BR112012023875A2 BR 112012023875 A2 BR112012023875 A2 BR 112012023875A2 BR 112012023875 A BR112012023875 A BR 112012023875A BR 112012023875 A BR112012023875 A BR 112012023875A BR 112012023875 A2 BR112012023875 A2 BR 112012023875A2
Authority
BR
Brazil
Prior art keywords
acromopeptidase
room temperature
sample
lysis
absence
Prior art date
Application number
BR112012023875A
Other languages
English (en)
Inventor
Becton Dickinson And Company
Danielle Hilligoss
Jamie Coady
Lisa M Keller
Samah Ramadan
Tobin J Hellyer
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of BR112012023875A2 publication Critical patent/BR112012023875A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

uso de acromopeptidase para lise em temperatura ambiente. um processo para detectar a presença ou ausência de bactérias gram positivas em uma amostra biológica. a amostra biológica pode ser obtida a partir de qualquer mamífero e contém, em um mínimo, componentes celulares. a amostra é combinada com um agente de lise enzimática como acromopeptidase, e lisado em temperatura ambiente. óxido férrico é então adicionado à amostra contendo acromopeptidase. um campo magnético é aplicado á amostra e ácidos nucléicos são extraídos dos componentes celulares. ácidos nucleicos alvos, se presentes, são amplificados usando técnicas como realçao em cadeia da polimera-se (pcr) e então usados para detectar a presença ou ausência de bactérias gram positivas.staphylococcus aureus e streptococcus agalactiae são exemplos de bactérias alvos detectadas pelos métodos da presente invenção.
BR112012023875A 2010-03-16 2011-03-15 uso de acromopeptidase para lise em temperatura ambiente BR112012023875A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31431810P 2010-03-16 2010-03-16
PCT/US2011/028494 WO2011115975A2 (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature

Publications (1)

Publication Number Publication Date
BR112012023875A2 true BR112012023875A2 (pt) 2015-09-22

Family

ID=44649786

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012023875A BR112012023875A2 (pt) 2010-03-16 2011-03-15 uso de acromopeptidase para lise em temperatura ambiente

Country Status (8)

Country Link
US (1) US20110275090A1 (pt)
EP (1) EP2547767A4 (pt)
JP (1) JP2013521804A (pt)
CN (1) CN102869773A (pt)
AU (1) AU2011227452B2 (pt)
BR (1) BR112012023875A2 (pt)
CA (1) CA2793144A1 (pt)
WO (1) WO2011115975A2 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2502761B (en) 2011-03-09 2015-09-30 Becton Dickinson Infusion Therapy Systems Inc Method and internal control composition for determining the efficacy of enzymatic cell lysis
WO2012178187A1 (en) 2011-06-23 2012-12-27 Paul Yager Reagent patterning in capillarity-based analyzers and associated systems and methods
WO2013117968A1 (en) 2012-02-10 2013-08-15 Reametrix Inc. Methods for lysing bacteria from sample and isolating cellular components therefrom, and kits therefor
EP2948249A1 (en) 2013-01-22 2015-12-02 University of Washington through its Center for Commercialization Sequential delivery of fluid volumes and associated devices, systems and methods
EP3320966B1 (en) 2016-11-14 2022-02-16 Airbus Operations GmbH Autoclave and method for welding thermoplastic composite parts
EP3681895A4 (en) * 2017-09-13 2021-05-26 Becton, Dickinson and Company METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES
WO2021217173A1 (en) * 2020-04-21 2021-10-28 Siemens Healthcare Diagnostics Inc. Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8414273D0 (en) * 1984-06-05 1984-07-11 Oxoid Ltd Identifying streptococcal grouping
JP2589729B2 (ja) * 1988-01-30 1997-03-12 極東製薬工業株式会社 染色体ｄｎａを用いた細菌の同定方法および該同定用キット
WO1992008133A1 (en) * 1990-10-29 1992-05-14 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
US5973138A (en) * 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
JP3172917B2 (ja) * 1999-07-23 2001-06-04 北海道 細菌検出方法
US20040002594A1 (en) * 2002-06-27 2004-01-01 John Krupey Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids
US7595176B2 (en) * 2003-05-30 2009-09-29 Georgia Tech Research Corporation Methods and reagents for quantitative analysis of Dehalococcoides species
US8298758B2 (en) * 2003-12-26 2012-10-30 Prima Meat Packers, Ltd. Method of multiplex microorganism detection
JP2008507296A (ja) * 2004-07-26 2008-03-13 ナノスフェアー　インコーポレイテッド 混合培養物中でメチシリン耐性黄色ブドウ球菌とメチシリン感受性黄色ブドウ球菌とを区別する方法
WO2007010641A1 (ja) * 2005-07-21 2007-01-25 Kyushu University 微生物検出法及び微生物検出キット
US20100099115A1 (en) * 2006-11-22 2010-04-22 Mach Patrick A Systems and methods for preparing and analyzing samples
JP2008148570A (ja) * 2006-12-14 2008-07-03 Hitachi Ltd 微生物検出システム
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090274577A1 (en) * 2008-05-05 2009-11-05 B-K Medical Aps Sterilization by treatment with reductant and oxidant

Also Published As

Publication number Publication date
US20110275090A1 (en) 2011-11-10
WO2011115975A2 (en) 2011-09-22
EP2547767A4 (en) 2013-12-25
AU2011227452B2 (en) 2015-04-09
AU2011227452A1 (en) 2012-10-04
CA2793144A1 (en) 2011-09-22
JP2013521804A (ja) 2013-06-13
CN102869773A (zh) 2013-01-09
EP2547767A2 (en) 2013-01-23
WO2011115975A3 (en) 2012-01-19

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Legal Events

Date Code Title Description
B15K Others concerning applications: alteration of classification

Ipc: C12N 15/10 (2006.01)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL