WO2012042374A3 - Method of determining number or concentration of molecules - Google Patents

Method of determining number or concentration of molecules Download PDF

Info

Publication number
WO2012042374A3
WO2012042374A3 PCT/IB2011/002438 IB2011002438W WO2012042374A3 WO 2012042374 A3 WO2012042374 A3 WO 2012042374A3 IB 2011002438 W IB2011002438 W IB 2011002438W WO 2012042374 A3 WO2012042374 A3 WO 2012042374A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
concentration
sample
determining
determining number
Prior art date
Application number
PCT/IB2011/002438
Other languages
French (fr)
Other versions
WO2012042374A2 (en
Inventor
Anssi Jussi Nikolai Taipale
Sten Linnarsson
Original Assignee
Anssi Jussi Nikolai Taipale
Sten Linnarsson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1016608.0A external-priority patent/GB201016608D0/en
Priority claimed from GBGB1022111.7A external-priority patent/GB201022111D0/en
Application filed by Anssi Jussi Nikolai Taipale, Sten Linnarsson filed Critical Anssi Jussi Nikolai Taipale
Publication of WO2012042374A2 publication Critical patent/WO2012042374A2/en
Publication of WO2012042374A3 publication Critical patent/WO2012042374A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of determining the number or concentration of molecules in a sample, a method of tracking a substance in a reaction or system, and applications of such methods. Use of nucleic acid as a tracer. The methods of determining the number or concentration involves either modifying the molecules with random oligonucleotide tags to create modified molecules which differ from each other; or taking a sample which is of such a small size that molecules in the sample will be different.
PCT/IB2011/002438 2010-10-01 2011-09-29 Method of determining number or concentration of molecules WO2012042374A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB1016608.0A GB201016608D0 (en) 2010-10-01 2010-10-01 Method of determining number or concentration of moecules
GB1016608.0 2010-10-01
GB1022111.7 2010-12-30
GBGB1022111.7A GB201022111D0 (en) 2010-12-30 2010-12-30 Method of determining number or concentration of molecules

Publications (2)

Publication Number Publication Date
WO2012042374A2 WO2012042374A2 (en) 2012-04-05
WO2012042374A3 true WO2012042374A3 (en) 2012-09-07

Family

ID=45044629

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/002438 WO2012042374A2 (en) 2010-10-01 2011-09-29 Method of determining number or concentration of molecules

Country Status (1)

Country Link
WO (1) WO2012042374A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9598736B2 (en) 2013-08-28 2017-03-21 Cellular Research, Inc. Massively parallel single cell analysis

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2572003A4 (en) 2010-05-18 2016-01-13 Natera Inc Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
ES2523140T3 (en) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Increased confidence in allele identifications with molecular count
BR112013020220B1 (en) 2011-02-09 2020-03-17 Natera, Inc. METHOD FOR DETERMINING THE PLOIDIA STATUS OF A CHROMOSOME IN A PREGNANT FETUS
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP2626433B1 (en) * 2012-02-09 2017-04-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for linking nucleic acids in a microsome from endoplasmatic reticuli
ES2776673T3 (en) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Methods and uses for molecular tags
CN104364392B (en) 2012-02-27 2018-05-25 赛卢拉研究公司 For the composition and kit of numerator counts
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
DK2828218T3 (en) 2012-03-20 2020-11-02 Univ Washington Through Its Center For Commercialization METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
CA2883901C (en) 2012-09-04 2023-04-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2912197B1 (en) 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
EP2749654A1 (en) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analysis of composition of nucleic acid mixtures
US9816088B2 (en) 2013-03-15 2017-11-14 Abvitro Llc Single cell bar-coding for antibody discovery
US10633691B2 (en) 2013-09-30 2020-04-28 Di Wu Methods to profile molecular complexes or single cells via proximity dependant barcoding
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
EP4389888A3 (en) 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
EP3087204B1 (en) 2013-12-28 2018-02-14 Guardant Health, Inc. Methods and systems for detecting genetic variants
KR102313470B1 (en) * 2014-02-05 2021-10-18 프라운호퍼-게젤샤프트 추르 푀르데룽 데어 안제반텐 포르슝 에 파우 Error-free sequencing of DNA
AU2015249846B2 (en) 2014-04-21 2021-07-22 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
CA2961210A1 (en) 2014-09-15 2016-03-24 Abvitro, Inc. High-throughput nucleotide library sequencing
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
US11535882B2 (en) 2015-03-30 2022-12-27 Becton, Dickinson And Company Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP4428863A2 (en) 2015-05-11 2024-09-11 Natera, Inc. Methods and compositions for determining ploidy
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
US10619186B2 (en) 2015-09-11 2020-04-14 Cellular Research, Inc. Methods and compositions for library normalization
EP3387152B1 (en) 2015-12-08 2022-01-26 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
CN108603228B (en) 2015-12-17 2023-09-01 夸登特健康公司 Method for determining tumor gene copy number by analyzing cell-free DNA
EP3452614B1 (en) 2016-05-02 2023-06-28 Becton, Dickinson and Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP4407625A3 (en) 2016-05-26 2024-10-23 Becton, Dickinson and Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
SG11201903158RA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for cell label classification
US11164659B2 (en) 2016-11-08 2021-11-02 Becton, Dickinson And Company Methods for expression profile classification
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10722880B2 (en) 2017-01-13 2020-07-28 Cellular Research, Inc. Hydrophilic coating of fluidic channels
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
CN111492068A (en) 2017-12-19 2020-08-04 贝克顿迪金森公司 Particles associated with oligonucleotides
EP3781714A1 (en) 2018-04-14 2021-02-24 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
ES2945191T3 (en) 2018-05-03 2023-06-29 Becton Dickinson Co High-throughput multi-omics sample analysis
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
BR112021000409A2 (en) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. METHODS AND REAGENTS TO CHARACTERIZE GENOMIC EDITION, CLONAL EXPANSION AND ASSOCIATED APPLICATIONS
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116635533A (en) 2020-11-20 2023-08-22 贝克顿迪金森公司 Profiling of high and low expressed proteins
WO2024003114A1 (en) 2022-06-29 2024-01-04 Actome Gmbh Detection of biomolecules in single cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008025656A1 (en) * 2008-05-28 2009-12-03 Genxpro Gmbh Method for quantitative analysis of nicoleic acids, markers therefor and their use
WO2010021936A1 (en) * 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US20110160078A1 (en) * 2009-12-15 2011-06-30 Affymetrix, Inc. Digital Counting of Individual Molecules by Stochastic Attachment of Diverse Labels

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008025656A1 (en) * 2008-05-28 2009-12-03 Genxpro Gmbh Method for quantitative analysis of nicoleic acids, markers therefor and their use
WO2010021936A1 (en) * 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US20110160078A1 (en) * 2009-12-15 2011-06-30 Affymetrix, Inc. Digital Counting of Individual Molecules by Stochastic Attachment of Diverse Labels

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A. M. SMITH ET AL: "Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples", NUCLEIC ACIDS RESEARCH, vol. 38, no. 13, 11 May 2010 (2010-05-11), pages E142 - E142, XP055017285, ISSN: 0305-1048, DOI: 10.1093/nar/gkq368 *
GLENN K FU ET AL: "Counting individual DNA molecules by the stochastic attachment of diverse labels", PNAS, 31 May 2011 (2011-05-31), pages 9026 - 9031, XP055017200, Retrieved from the Internet <URL:http://www.pnas.org/content/108/22/9026.full.pdf#page=1&view=FitH> [retrieved on 20120123], DOI: 10.1073/pnas.1017621108/-/DCSupplemental *
HUBERT HUG ET AL: "Measurement of the number of molecules of a single mRNA species in a complex mRNA preparation.", JOURNAL OF THEORETICAL BIOLOGY, vol. 221, no. 4, 1 April 2003 (2003-04-01), pages 615 - 624, XP055017448, ISSN: 0022-5193 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9598736B2 (en) 2013-08-28 2017-03-21 Cellular Research, Inc. Massively parallel single cell analysis

Also Published As

Publication number Publication date
WO2012042374A2 (en) 2012-04-05

Similar Documents

Publication Publication Date Title
WO2012042374A3 (en) Method of determining number or concentration of molecules
EP4219745A3 (en) Spatially encoded biological assays using a microfluidic device
MX2015001939A (en) Microcapsule compositions and methods.
WO2013154999A3 (en) Method of preparation of nanopore and uses thereof
WO2015089511A3 (en) Modified nucleic acid molecules and uses thereof
MX2018013367A (en) Methods for tagging dna-encoded libraries.
WO2012166742A3 (en) Systems and methods for genetic and biological analysis
WO2015002677A8 (en) Alkaline activation for immobilization of dna taggants
WO2014028378A3 (en) Methods and systems for detecting biological components
WO2012058638A3 (en) Nucleic acid nanostructure barcode probes
MX365093B (en) Systems and methods for detection of cells using engineered transduction particles.
WO2013050881A3 (en) Direct nucleic acid analysis
MX2014005009A (en) Nanoparticle smart tags in subterranean applications.
WO2014152281A3 (en) Ribosomal ribonucleic acid hybridization for organism identification
EP2314680A4 (en) Method for amplification of target nucleic acid sequence, method for detection of mutation by using the method, and reagents for use in the methods
WO2011011305A3 (en) A system and method for identifying and providing user-specific psychoactive content
WO2011127001A3 (en) Quantum dot-based optical sensors for rapid detection and quantitative analysis of biomolecules and biological materials
CL2013001621A1 (en) Nucleic acid regulatory molecule with definite specificity, expression construction comprising it, vector; microorganism, plant or animal cell; and method for the production of said molecule.
GB2515944A (en) Peptide arrays
WO2014059352A3 (en) Polymers having orthogonal reactive groups and uses thereof
WO2012155097A3 (en) Systems and methods for producing an evaporation barrier in a reaction chamber
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
WO2012092382A3 (en) Role of microrna in t cell immune response
BR112016010159A2 (en) uses of a nucleic acid pool and microarray chip, microarray chip, methods for detecting beneficial pathogens and / or microorganisms in an organic sample and treating a diseased grass, and
WO2012113476A3 (en) Method and system for displaying content on a display of a client

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11788224

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11788224

Country of ref document: EP

Kind code of ref document: A2