CN102845478A - Trichoderma harzianum chlamydospore granules and preparation method thereof - Google Patents
Trichoderma harzianum chlamydospore granules and preparation method thereof Download PDFInfo
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- CN102845478A CN102845478A CN201210291951XA CN201210291951A CN102845478A CN 102845478 A CN102845478 A CN 102845478A CN 201210291951X A CN201210291951X A CN 201210291951XA CN 201210291951 A CN201210291951 A CN 201210291951A CN 102845478 A CN102845478 A CN 102845478A
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Abstract
The invention discloses trichoderma harzianum chlamydospore granules and a preparation method thereof. The trichoderma harzianum chlamydospore granules comprise a vegetative mass having a mass-volume ratio of 13Kg: 10L, and a fresh fermentation bacterium liquid prepared from trichoderma harzianum, wherein the trichoderma harzianum is prepared by uniformly mixing diatomite and bran according to a mass ratio. The preparation method comprises the following steps of uniformly mixing the vegetative mass and the fresh fermentation bacterium liquid prepared from trichoderma harzianum by stirring, taking a wet semi-finished product, and carrying out drying crushing by a drying pulverizer. The trichoderma harzianum chlamydospore granules have chlamydospore content of 108cfu/mL, a low price, a long shelf life, strong stress resistance and low production cost, independently provides nutrients to soil, prevents external factor interference, and is suitable for mechanized application. The preparation method of the trichoderma harzianum chlamydospore granules has the advantages of low production cost, simple production processes, long product shelf life, strong stress resistance and good environmental benefits.
Description
Technical field
The present invention relates to biological field, in particular, relate to a kind of Trichoderma chlamydospore preparation and preparation method thereof.
Background technology
Trichoderma, as a kind of microorganism that extensively is present in occurring in nature, since energy Adsorption of Heavy Metals, degrading pesticide residues and cyanide pollution etc., Chang Zuowei restoration of the ecosystem agent.Can produce multiple bioactivator (such as cell wall degradation enzyme and secondary metabolite) with antagonism during in addition owing to Trichoderma and plant symbiosis, can promote growing and inducing and the inducing plant autoimmune function of plant, so the biological control agents of its Chang Zuowei plant disease is extensively concerned.So far, utilize Trichoderma to carry out the existing nearly 80 years history of biological control research, its disease-resistant short fruit of coming into force has obtained extensively certainly, is the widely used biocontrol microorganisms in the whole world.Nowadays produce upper trichoderma commonly used and mostly be the conidium preparation, the conidium preparation that soon Trichoderma fermentation is made carries out seed pelleting or soil treatment.Had in the world more than 120 kind of Trichoderma commercialization preparation to put goods on the market, but the active ingredient of these Trichoderma preparations mostly is conidium.
Because Trichoderma conidium preparation, shelf life (racks store time) is short, and germination rate is low, a little less than the anti-adversity ability.Therefore in soil, be subject to easily the impact of complicated factor, and under the overall situation of modern mechanical production, the effect of practical application is not very good.
Summary of the invention
For the technical problem that exists in the above-mentioned prior art, the invention provides a kind of Trichoderma chlamydospore granule, cheap, have shelf life length, high-output stress-resistance, the characteristics such as production cost is low, and realized in soil, providing autotrophy, prevented the external factor interference, and the triple purposes that are adapted to mechanized application.
The present invention also provides the preparation method of above-mentioned Trichoderma chlamydospore granule, and production cost is low, production technology is simple, product shelf phase length, high-output stress-resistance, environmentally friendly.
To achieve the above object of the invention, the technical solution adopted in the present invention is as follows:
A kind of Trichoderma chlamydospore granule comprises: mass volume ratio is a kind of trophosome of 13 Kg:10L and fresh zymocyte liquid by trichoderma strain preparation, and wherein said trophosome is to be that diatomite and the wheat bran of 10:3 evenly mixes by mass ratio.
Described trichoderma strain comprises the antagonistic Trichoderma bacterial strain, trichoderma harzianum strain (
Trichoderma harzianumSH2303).
The preparation method of above-mentioned Trichoderma chlamydospore granule, step is as follows:
(1) takes by weighing respectively diatomite and wheat bran according to mass ratio, mix the making trophosome;
(2) prepare fresh zymocyte liquid by trichoderma strain;
(3) according to mass ratio with described trophosome and described zymocyte liquid stirring and evenly mixing, then take out moistening semi-finished product, through drying and crushing machine drying and crushing.
The preparation method of described zymocyte liquid is as follows: with the Trichoderma inoculation on the PDA medium, cultivate after 3 days, beat from colony edge with card punch (diameter 0.5 cm) and to get 3～5 of bacterium sheets, be inoculated in the PDA liquid nutrient medium, under 28 ℃, 140 r/min shaking table conditions, cultivated 5 days, obtain the Trichoderma zymotic fluid.
The purpose that the present invention can realize preventing and treating soil-borne disease and promote plant growth by above-mentioned trichoderma strain, overcome Traditional Wood mould conidium preparation, shelf life is short, the characteristics that anti-adversity ability is weak, greatly improved the storage time of Trichoderma biological prevention and control agent, reduced production cost, using for Trichoderma biological prevention and control agent large tracts of land provides possibility.
Description of drawings
Fig. 1 is Trichoderma chlamydospore granule schematic diagram disclosed in this invention;
Fig. 2 is at the forward and backward schematic diagram that affects on emergence of corn rate and plant height of the Trichoderma chlamydospore granule of application drawing 1.
Embodiment
Below in conjunction with the drawings and specific embodiments technical scheme of the present invention is elaborated; present embodiment is implemented under take technical solution of the present invention as prerequisite; provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Shown in Figure 1 is a kind of Trichoderma chlamydospore granule schematic diagram provided by the present invention, and in the present embodiment, this spore granule is to be mixed by fresh zymocyte liquid 10 L that the trophosome of 13 kg and antagonistic Trichoderma bacterial strain prepare to form.Wherein trophosome is to be that the diatomite of 10 kg and wheat bran that quality is 3 kg evenly mix by quality.
The preparation method of Trichoderma chlamydospore granule shown in Figure 1 is:
At first, take by weighing diatomite and wheat bran that mass ratio is 10:3, both are evenly mixed, make trophosome;
Secondly, with the Antagonistic Trichoderma inoculation on the PDA medium, cultivate after 3 days, beat from colony edge with card punch (diameter 0.5 cm) and to get 3～5 of bacterium sheets, be inoculated in the PDA liquid nutrient medium, under 28 ℃, 140 r/min shaking table conditions, cultivated 5 days, obtain the fresh zymocyte liquid of Antagonistic Trichoderma;
At last, according to mass volume ratio be 13 Kg:10L with above-mentioned trophosome and zymocyte liquid stirring and evenly mixing, then take out moistening semi-finished product, through drying and crushing machine drying and crushing.
The Trichoderma chlamydospore granule chlamydospore content of being prepared by the inventive method reaches 10
8Cfu/mL can use at the different times of crop.For example, can before corn seeding, use Trichoderma chlamydospore granule.Trichoderma chlamydospore granule is spread fertilizer over the fields before Mechanization sowing in the earth's surface, etc. instituting an inquiry emergence of corn rate and corn plant height after the emergence of corn.As shown in Figure 2, the histogram on the left side represents not use emergence of corn rate and the plant height of Trichoderma chlamydospore granule, and the histogram on the right represents to use emergence of corn rate and the plant height behind the Trichoderma chlamydospore granule.The result shows, use Trichoderma chlamydospore granule after, emergence of corn rate and plant height all are significantly improved.
Claims (4)
1. Trichoderma chlamydospore granule, it is characterized in that, comprise: mass volume ratio is a kind of trophosome of 13 Kg:10L and fresh zymocyte liquid by trichoderma strain preparation, and wherein said trophosome is to be that diatomite and the wheat bran of 10:3 evenly mixes by mass ratio.
2. Trichoderma chlamydospore granule according to claim 1 is characterized in that described trichoderma strain comprises antagonistic Trichoderma bacterial strain and trichoderma harzianum strain.
3. the preparation method of Trichoderma chlamydospore granule according to claim 1, it is characterized in that step is as follows: (1) takes by weighing respectively diatomite and wheat bran according to mass ratio, mixes the making trophosome;
(2) prepare fresh zymocyte liquid by trichoderma strain;
(3) according to mass ratio with described trophosome and described zymocyte liquid stirring and evenly mixing, then take out moistening semi-finished product, through drying and crushing machine drying and crushing.
4. the preparation method of Trichoderma chlamydospore granule according to claim 3, it is characterized in that, the preparation method of described zymocyte liquid is as follows: with the Trichoderma inoculation on the PDA medium, cultivate after 3 days, beat from colony edge with card punch and to get 3～5 of bacterium sheets, be inoculated in the PDA liquid nutrient medium, under 28 ℃, 140 r/min shaking table conditions, cultivated 5 days, obtain the Trichoderma zymotic fluid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210291951XA CN102845478A (en) | 2012-08-16 | 2012-08-16 | Trichoderma harzianum chlamydospore granules and preparation method thereof |
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| CN201210291951XA CN102845478A (en) | 2012-08-16 | 2012-08-16 | Trichoderma harzianum chlamydospore granules and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555648A (en) * | 2013-08-27 | 2014-02-05 | 云南省烟草公司临沧市公司 | Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore |
| CN104788175A (en) * | 2015-03-30 | 2015-07-22 | 上海交通大学 | Biological trichoderma viride granule as well as preparation method and application thereof |
Citations (5)
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|---|---|---|---|---|
| CN1283599A (en) * | 1999-08-09 | 2001-02-14 | 王井宝 | Quick-acting disease-resistant organic fungus fertilizer and its preparing process |
| CN1557164A (en) * | 2004-01-18 | 2004-12-29 | 沈阳农业大学 | Multifunctional biological control bacteria agent and its preparation method |
| CN1594544A (en) * | 2004-07-01 | 2005-03-16 | 上海交通大学 | Method for producing trichoderma chlamydosporium |
| CN101024816A (en) * | 2007-02-02 | 2007-08-29 | 沈阳农业大学 | Method for preparing Trichderma bacteria conididium powder and use thereof |
| CN102388922A (en) * | 2011-10-21 | 2012-03-28 | 上海交通大学 | Preparation method and application of wettable powder for preventing and controlling Sphaerotheca fuliginea trichoderma of greenhouse cucumbers |
-
2012
- 2012-08-16 CN CN201210291951XA patent/CN102845478A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1283599A (en) * | 1999-08-09 | 2001-02-14 | 王井宝 | Quick-acting disease-resistant organic fungus fertilizer and its preparing process |
| CN1557164A (en) * | 2004-01-18 | 2004-12-29 | 沈阳农业大学 | Multifunctional biological control bacteria agent and its preparation method |
| CN1594544A (en) * | 2004-07-01 | 2005-03-16 | 上海交通大学 | Method for producing trichoderma chlamydosporium |
| CN101024816A (en) * | 2007-02-02 | 2007-08-29 | 沈阳农业大学 | Method for preparing Trichderma bacteria conididium powder and use thereof |
| CN102388922A (en) * | 2011-10-21 | 2012-03-28 | 上海交通大学 | Preparation method and application of wettable powder for preventing and controlling Sphaerotheca fuliginea trichoderma of greenhouse cucumbers |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555648A (en) * | 2013-08-27 | 2014-02-05 | 云南省烟草公司临沧市公司 | Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore |
| CN104788175A (en) * | 2015-03-30 | 2015-07-22 | 上海交通大学 | Biological trichoderma viride granule as well as preparation method and application thereof |
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Application publication date: 20130102 |