CN102816819B - 提高人胰岛素前体融合蛋白酶切转换成b30缺苏氨酸人胰岛素产率的方法 - Google Patents
提高人胰岛素前体融合蛋白酶切转换成b30缺苏氨酸人胰岛素产率的方法 Download PDFInfo
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CN103290083B (zh) * | 2013-07-04 | 2015-05-06 | 珠海联邦制药股份有限公司 | 一种人胰岛素酯的制备方法 |
CN105802989B (zh) * | 2014-12-31 | 2020-05-19 | 江苏众红生物工程创药研究院有限公司 | 毕赤酵母表达重组蛋白的载体、基因、方法及应用 |
CN105153294B (zh) * | 2015-08-31 | 2019-02-12 | 济南康和医药科技有限公司 | 一种重组胰岛素及胰岛素类似物前体纯化方法 |
CN105111304B (zh) * | 2015-09-30 | 2018-12-14 | 山东阿华生物药业有限公司 | 重组人胰岛素前体的纯化和酶切转换方法 |
CN106749616B (zh) * | 2016-12-19 | 2021-09-03 | 华润昂德生物药业有限公司 | B30位苏氨酸缺失人胰岛素晶体的制备方法 |
CN108164594B (zh) * | 2017-12-08 | 2020-03-31 | 珠海冀百康生物科技有限公司 | 一种去b链30位氨基酸残基的胰岛素前体沉淀的回收方法 |
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CN1059703C (zh) * | 1993-04-10 | 2000-12-20 | 北京大学 | 人胰岛素前体基因在大肠杆菌中的直接表达及后加工方法 |
CN1125081C (zh) * | 1999-09-08 | 2003-10-22 | 中国科学院上海生物化学研究所 | 重组天然和新型人胰岛素及其制备方法 |
CN100424179C (zh) * | 2001-11-19 | 2008-10-08 | 诺沃挪第克公司 | 制备胰岛素化合物的方法 |
EP1907419B1 (en) * | 2005-07-08 | 2011-01-26 | Biocon Limited | Preparation of insulin conjugates |
CA2690565A1 (en) * | 2007-06-13 | 2008-12-24 | Sterrenbeld Biotechnologie North America, Inc. | Process for producing exogenous protein in the milk of transgenic mammals |
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Address after: Shandong city of Liaocheng province Dong'e 252201 Jiao County Street No. 78 Co-patentee after: EAST CHINA University OF SCIENCE AND TECHNOLOGY Patentee after: Huarun Onde Biopharmaceutical Co.,Ltd. Co-patentee after: SHAN DONG DONG-E E-JIAO Co.,Ltd. Address before: Shandong city of Liaocheng province Dong'e 252201 Jiao County Street No. 78 Co-patentee before: EAST CHINA University OF SCIENCE AND TECHNOLOGY Patentee before: ANGDE BIOLOGY PHARMACEUTICAL CO.,LTD. Co-patentee before: SHAN DONG DONG-E E-JIAO Co.,Ltd. Address after: Shandong city of Liaocheng province Dong'e 252201 Jiao County Street No. 78 Co-patentee after: EAST CHINA University OF SCIENCE AND TECHNOLOGY Patentee after: ANGDE BIOLOGY PHARMACEUTICAL CO.,LTD. Co-patentee after: SHAN DONG DONG-E E-JIAO Co.,Ltd. Address before: Shandong city of Liaocheng province Dong'e 252201 Jiao County Street No. 78 Co-patentee before: EAST CHINA University OF SCIENCE AND TECHNOLOGY Patentee before: SHANDONG EHUA BIOLOGICAL PHARMACEUTICAL Co.,Ltd. Co-patentee before: SHAN DONG DONG-E E-JIAO Co.,Ltd. |
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