CN102803491A - 用于在酵母细胞表面上展示多肽的方法和组合物 - Google Patents

用于在酵母细胞表面上展示多肽的方法和组合物 Download PDF

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CN102803491A
CN102803491A CN2011800148893A CN201180014889A CN102803491A CN 102803491 A CN102803491 A CN 102803491A CN 2011800148893 A CN2011800148893 A CN 2011800148893A CN 201180014889 A CN201180014889 A CN 201180014889A CN 102803491 A CN102803491 A CN 102803491A
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polypeptide
nucleic acid
antibody polypeptide
fragment
antibody
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S.里卡尔特
G.莱隆德尔
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Oxyrane UK Ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800148893A 2010-01-21 2011-01-21 用于在酵母细胞表面上展示多肽的方法和组合物 Pending CN102803491A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29709310P 2010-01-21 2010-01-21
US61/297,093 2010-01-21
PCT/IB2011/000227 WO2011089527A1 (en) 2010-01-21 2011-01-21 Methods and compositions for displaying a poypeptide on a yeast cell surface

Publications (1)

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CN102803491A true CN102803491A (zh) 2012-11-28

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US (1) US20130096281A1 (https=)
EP (1) EP2526193A1 (https=)
JP (1) JP2013517761A (https=)
KR (1) KR20120118045A (https=)
CN (1) CN102803491A (https=)
BR (1) BR112012018163A2 (https=)
CA (1) CA2787677A1 (https=)
SG (1) SG182647A1 (https=)
WO (1) WO2011089527A1 (https=)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105722387A (zh) * 2013-09-18 2016-06-29 瑞泽恩制药公司 组氨酸工程改造的轻链抗体和用于生成所述抗体的经基因修饰的非人动物
CN106701606A (zh) * 2016-12-19 2017-05-24 广东启智生物科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
CN108085332A (zh) * 2017-12-27 2018-05-29 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法
CN114606151A (zh) * 2022-04-27 2022-06-10 南京工业大学 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用
CN115851460A (zh) * 2022-08-10 2023-03-28 丁辰 一种具有激活哺乳动物干细胞分化功能的菌株、组合物及其应用

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008120107A2 (en) 2007-04-03 2008-10-09 Oxyrane (Uk) Limited Glycosylation of molecules
US9598682B2 (en) 2009-09-29 2017-03-21 Vib Vzw Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
US20130053550A1 (en) 2009-11-19 2013-02-28 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
WO2012042387A2 (en) 2010-09-29 2012-04-05 Oxyrane Uk Limited De-mannosylation of phosphorylated n-glycans
US9347050B2 (en) 2010-09-29 2016-05-24 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated N-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
EP2739733B1 (en) * 2011-08-03 2017-11-22 Texas Biomedical Research Insitute Nucleic acid compositions, methods and kits for rapid pairing of affinity agents
EP3628326B1 (en) 2012-03-15 2024-02-28 Oxyrane UK Limited Methods and materials for treatment of pompe's disease
CN113354744A (zh) * 2013-12-20 2021-09-07 弗雷德哈钦森癌症研究中心 带标签的嵌合效应分子及其受体
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
US20160185633A1 (en) * 2014-12-30 2016-06-30 University Of Florida Research Foundation, Inc. Recovery of nutrients from water and wastewater by precipitation as struvite
EP3288570A4 (en) 2015-04-29 2018-11-21 Fred Hutchinson Cancer Research Center Modified stem cells and uses thereof
CN108779451A (zh) * 2016-01-08 2018-11-09 帝斯曼知识产权资产管理有限公司 解脂耶氏酵母的交配型转换
US10988759B2 (en) 2016-01-15 2021-04-27 University Of Washington High throughput protein-protein interaction screening in yeast liquid culture
FR3081169B1 (fr) 2018-05-15 2020-06-19 Messenger Biopharma Substitution de la coiffe des arn messagers par deux sequences d'arn introduites a leur extremite 5'
IL298006B2 (en) * 2020-05-11 2024-10-01 A Alpha Bio Inc High-throughput screening methods to identify small molecule targets
IL298616B2 (en) 2020-06-01 2024-11-01 A Alpha Bio Inc Methods for characterizing and engineering protein-protein interactions
WO2023051972A1 (en) 2021-09-29 2023-04-06 Miltenyi Biotec B.V. & Co. KG Method for the generation and selection of a producer cell
CN115786387B (zh) * 2022-09-19 2024-02-13 苏州泓迅生物科技股份有限公司 一种通用型酵母细胞表面展示质粒载体及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999036569A1 (en) * 1998-01-20 1999-07-22 The Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
WO2003029456A1 (en) * 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US20080171359A1 (en) * 2005-04-14 2008-07-17 Oxyrane (Uk) Ltd. Recombinant Yeasts for Synthesizing Epoxide Hydrolases
WO2008100816A2 (en) * 2007-02-09 2008-08-21 Medimmune, Llc Antibody libray display by yeast cell plasma membrane

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6042828A (en) 1992-09-07 2000-03-28 Kyowa Hakko Kogyo Co., Ltd. Humanized antibodies to ganglioside GM2
US6699658B1 (en) * 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US7442772B2 (en) 1997-12-03 2008-10-28 Genentech, Inc. Antibodies to PRO361 polypeptide
WO2001042462A2 (en) 1999-12-08 2001-06-14 National University Of Singapore Phospholipase a2 inhibitory peptides from python reticulatus
ES2327830T3 (es) 2002-03-29 2009-11-04 Schering Corporation Anticuerpos monoclonales humanos anti-interleuquina-5 y metodos y composiciones que los contienen.
CA2501422C (en) 2004-04-29 2014-08-12 University Of Rochester Lymphoid chemokines in the diagnosis, monitoring and treatment of autoimmune disease
US7431927B2 (en) 2005-03-24 2008-10-07 Epitomics, Inc. TNFα-neutralizing antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999036569A1 (en) * 1998-01-20 1999-07-22 The Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
WO2003029456A1 (en) * 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US20080171359A1 (en) * 2005-04-14 2008-07-17 Oxyrane (Uk) Ltd. Recombinant Yeasts for Synthesizing Epoxide Hydrolases
WO2008100816A2 (en) * 2007-02-09 2008-08-21 Medimmune, Llc Antibody libray display by yeast cell plasma membrane

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BODER ET AL.: "Yeast surface dispaly for screening combination polypeptide libraries", 《NATURE BIOTECHNOLOGY》, vol. 15, 30 June 1997 (1997-06-30), pages 553 - 557 *
LIXI YUE ET AL.: "Construction of a new plasmid for surface display oncells of Yarrowia lipolytica", 《JOURNAL OF MICROBIOLOGICAL METHODS》, vol. 72, no. 2, 29 February 2008 (2008-02-29), pages 116 - 123, XP022420000 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105722387A (zh) * 2013-09-18 2016-06-29 瑞泽恩制药公司 组氨酸工程改造的轻链抗体和用于生成所述抗体的经基因修饰的非人动物
CN105722387B (zh) * 2013-09-18 2020-01-17 瑞泽恩制药公司 组氨酸工程改造的轻链抗体和用于生成所述抗体的经基因修饰的非人动物
CN106701606A (zh) * 2016-12-19 2017-05-24 广东启智生物科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
CN108085332A (zh) * 2017-12-27 2018-05-29 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
CN108085332B (zh) * 2017-12-27 2020-11-27 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法
CN114606151A (zh) * 2022-04-27 2022-06-10 南京工业大学 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用
CN114606151B (zh) * 2022-04-27 2023-05-19 南京工业大学 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用
CN115851460A (zh) * 2022-08-10 2023-03-28 丁辰 一种具有激活哺乳动物干细胞分化功能的菌株、组合物及其应用

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KR20120118045A (ko) 2012-10-25
CA2787677A1 (en) 2011-07-28
JP2013517761A (ja) 2013-05-20
WO2011089527A1 (en) 2011-07-28
US20130096281A1 (en) 2013-04-18
SG182647A1 (en) 2012-08-30
BR112012018163A2 (pt) 2019-09-24
EP2526193A1 (en) 2012-11-28

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Application publication date: 20121128