CN102783656A - Application of shiny-leaved yellowhorn flavone in lipid-lowering weight-losing field and preparation method of shiny-leaved yellowhorn flavone - Google Patents
Application of shiny-leaved yellowhorn flavone in lipid-lowering weight-losing field and preparation method of shiny-leaved yellowhorn flavone Download PDFInfo
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- CN102783656A CN102783656A CN2012103214041A CN201210321404A CN102783656A CN 102783656 A CN102783656 A CN 102783656A CN 2012103214041 A CN2012103214041 A CN 2012103214041A CN 201210321404 A CN201210321404 A CN 201210321404A CN 102783656 A CN102783656 A CN 102783656A
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Abstract
The invention aims at providing a preparation method of flavone in shiny-leaved yellowhorn shells and shiny-leaved yellowhorn flavone which has efficient lipid-lowering weight-losing effects. The preparation method of flavone is efficient, high in purity and yield, short in time consumption, simple to operate and low in production cost and corrosion of devices. The flavone can be used in the lipid-lowering weight-losing field. The flavone is added in functional food according to a certain proportion so as to obtain shiny-leaved yellowhorn flavone functional food with good effects. The technical scheme of the flavone includes: immersing and extracting the smashed shiny-leaved yellowhorn shells by using a certain amount of solvent, and filtering; and performing column chromatography after concentration, collecting parts of eluent, and performing freeze-drying to obtain the shiny-leaved yellowhorn flavone. The flavone is added in food according to a certain proportion so as to have lipid-lowering weight-losing effects.
Description
Technical field
The present invention provides shiny-leaved yellowhorn flavones application in the lowering blood-fat and reducing weight field and preparation method thereof, belongs to agro-processing technology, relevant lowering blood-fat and reducing weight such as functional food field.
Background technology
Shiny-leaved yellowhorn belongs to the Sapindaceae shiny-leaved yellowhorn and belongs to, and also is referred to as civil official fruit, xanthoceras sobifolia bunge etc., is the woody seeds of the distinctive preciousness of China.It is very high that shinyleaf yellowhorn shell contains the flavones amount, and colorimetric estimation reaches 4%.The shiny-leaved yellowhorn flavones belongs to the oleanane type flavones, is prevalent in the plants such as pulse family, Araliaceae, Carrot family, Primulaceae, Curcurbitaceae.Flavone compound aboundresources and be easy to extract extensively exists in organs such as the stem of plant, leaf, flower, fruit, be one group at structure and different in nature materials, the platymiscium secondary metabolite.The shinyleaf yellowhorn shell flavones has the effect of anti-anoxic, anti-oxidant, antifatigue.
Flavone compound adopts solvent extraction usually.Mixed solvent such as methanol-water that the general available water of flavones, methyl alcohol, ethanol, acetone or polarity are bigger extract.Though technology is stablized feasible, efficient is low, length consuming time, and the reagent consumption is big, and solvent is prone to residual, and environment is had certain infringement.Pancreatic lipase is to absorb fatty most important enzyme in the digestive system; Food fat decomposes under the effect of pancreatic lipase in digesting and assimilating process, generates aliphatic acid and 2-acylglycerol; These steatolysis products mix with bile salt, and dispersion could be by little intestinal absorption after forming particulate.If can suppress the pancreatic lipase activity, just can postpone and reduce the absorption of small intestine, thereby reach anti-fat effect for reducing fat food fat.Yet, be that the fat-weight-reducing food of composition does not have report as yet at present with the shiny-leaved yellowhorn flavones.
Summary of the invention
The purpose of this invention is to provide a kind of efficient, purity is high, yield is high, weak point consuming time, simple to operate, production cost is low, to flavones preparation method in the low shinyleaf yellowhorn shell of equipment corrosion and have efficient lowering blood-fat and reducing weight effect shiny-leaved yellowhorn flavones; This flavones can be used for the lowering blood-fat and reducing weight field; For example join by a certain percentage in the functional food, can obtain respond well shiny-leaved yellowhorn flavones functional food field.
Its technical scheme is: the shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof; It is characterized in that adopting following steps: (1) shinyleaf yellowhorn fruit shell is pulverized; Use the solution of the volume of 60%-70% as 10-20 times of milliliter of shinyleaf yellowhorn shell opaque amount gram number; 70-75 ℃ of following lixiviate 120～240 minutes filtered; (2) filtrating is condensed into paste and crosses column chromatography, collect eluent, eluent is concentrated freeze-dried, promptly get the shiny-leaved yellowhorn flavones; (4) the shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight functional food field: the ratio in 0.5%-1.1% (mass ratio) joins in the food, and food comprises bread and cheese and health food, products such as optional capsule, pulvis.
Described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and shinyleaf yellowhorn shell is pulverized order and counted the 5-20 order in the step 1, and shinyleaf yellowhorn shell needs degreasing degreasing.
Described shiny-leaved yellowhorn flavones contains 5%-10% methyl alcohol in the application in lowering blood-fat and reducing weight field and preparation method thereof in the used solution in the step 1.
Described shiny-leaved yellowhorn flavones concentrates in the step 2 and can adopt decompression to concentrate in the application in lowering blood-fat and reducing weight field and preparation method thereof; The decompression thickening temperature is 60～70 ℃, under 0.1～0.7 atmospheric pressure; Also can adopt the normal pressure heating.
Described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and used filler is the X-8 macroreticular resin in the step 3, and eluent is 75% ethanol.
Described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, wants mixing and stirring after adding functional food in the step 4 to.
Described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and this functional food lowering blood-fat and reducing weight mode is to be approach to suppress the pancreatic lipase activity in the step 4.
The present invention compared with prior art, the mixed solution that the technology that is adopted has used ethanol and methyl alcohol to form has reduced the consumption of methyl alcohol, has reduced dissolvent residual, has improved the extraction performance of solvent, has improved the recovery rate of flavones.
Compare with traditional handicraft, technology is simple, and recovery rate is high (to be compared with simple ethanol extraction; Recovery rate has increased about 20%), yield high (compare with simple ethanol extraction, yield has improved about 18%); Methanol usage significantly reduces (under the identical recovery rate, than simple methyl alcohol extraction, methanol usage has reduced about 33%); Production process is simple, and is easy to operate, is prone to industrialization, mechanization, automated production.And the shiny-leaved yellowhorn flavones that the method obtains is active through suppressing pancreatic lipase, can realize functions of lowering blood-fat and reducing weight preferably.The shiny-leaved yellowhorn flavones is through testing discovery to active inhibition of pancreatic lipase, and the shiny-leaved yellowhorn flavones can suppress the pancreatic lipase activity and reach 40%, proves tangible functions of lowering blood-fat and reducing weight.
Preferred forms embodiment 1: shinyleaf yellowhorn fruit shell is crushed to the 5-20 order after the degreasing, and with ethanol content 20%, 500 milliliters of the solution of methanol content 10% soak 50g shinyleaf yellowhorn shell powder, and 70 ℃ of following lixiviates 120 minutes are filtered; (2) will filtrate under the normal pressure after boiling removes about many milliliters of ethanol, water and methyl alcohol to 150; Change decompression into and concentrate, it is 70 ℃ that decompression concentrates condition, 0.1 atmospheric pressure; Shiny-leaved yellowhorn flavones medicinal extract is added on the column chromatography filler; Use 75% ethanol elution then, collect the part eluent, measuring flavones content after the eluent freeze-drying is 62%; (3) the shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight functional food field: the shiny-leaved yellowhorn flavones is joined mixing and stirring in the ground rice in the ratio of 0.5% (mass ratio), can suppress the pancreatic lipase activity and reach 25%.
Embodiment 2: shinyleaf yellowhorn fruit shell is crushed to the 5-20 order after the degreasing, and with ethanol content 40%, 1000 milliliters of the solution of methanol content 5% soak 50g shinyleaf yellowhorn shell powder, and 70 ℃ of following lixiviates 240 minutes are filtered; (2) will filtrate under the normal pressure after boiling removes about many milliliters of ethanol, water and methyl alcohol to 200; Change decompression into and concentrate, it is 70 ℃ that decompression concentrates condition, 0.1 atmospheric pressure; After being condensed into medicinal extract is added on the column chromatography filler; Use 75% ethanol elution then, collect the part eluent, measuring flavones content after the eluent freeze-drying is 67%; (3) the shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field: the shiny-leaved yellowhorn flavones is joined mixing and stirring in the bread and cheese in the ratio of 1.1% (mass ratio), can suppress the pancreatic lipase activity and reach 38 %.
Claims (7)
1. the shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof; It is characterized in that adopting following steps: (1) shinyleaf yellowhorn fruit shell is pulverized; The volume that uses 60%-70% is the shinyleaf yellowhorn shell opaque amount gram number 10-20 solution of milliliter doubly, and 70-75 ℃ of following lixiviate 120～240 minutes filtered; (2) filtrating is condensed into paste and crosses column chromatography, collect eluent, eluent is concentrated freeze-dried, promptly get the shiny-leaved yellowhorn flavones; (4) the shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight functional food field: the ratio in 0.5%-1.1% (mass ratio) joins in the food, and food comprises bread and cheese and health food, products such as optional capsule, pulvis.
2. described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and shinyleaf yellowhorn shell is pulverized order and counted the 5-20 order in the step 1, and shinyleaf yellowhorn shell needs degreasing degreasing.
3. described shiny-leaved yellowhorn flavones contains 5%-10% methyl alcohol in the application in lowering blood-fat and reducing weight field and preparation method thereof in the used solution in the step 1.
4. described shiny-leaved yellowhorn flavones concentrates in the step 2 and can adopt decompression to concentrate in the application in lowering blood-fat and reducing weight field and preparation method thereof; The decompression thickening temperature is 60～70 ℃, under 0.1～0.7 atmospheric pressure; Also can adopt the normal pressure heating.
5. described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and used filler is the X-8 macroreticular resin in the step 3, and eluent is 75% ethanol.
6. described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, wants mixing and stirring after adding functional food in the step 4 to.
7. described shiny-leaved yellowhorn flavones is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and this functional food lowering blood-fat and reducing weight mode is to be approach to suppress the pancreatic lipase activity in the step 4.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918800A (en) * | 2013-06-04 | 2014-07-16 | 北京农学院 | Application of xanthoceras sorbifolia oil to prepare anti-fatigue blend oil |
| CN104473157A (en) * | 2014-11-25 | 2015-04-01 | 马绍两 | Application of flavone extract of xanthoceras sorbifolia in sugar-reducing and fat-reducing fields and extraction method of flavone extract |
Citations (3)
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| CN101537052A (en) * | 2009-04-24 | 2009-09-23 | 山东大学 | Hubei caval vine general flavone extract as well as preparation method and application thereof |
| CN101974094A (en) * | 2010-07-26 | 2011-02-16 | 南京泽朗医药科技有限公司 | Method for comprehensively extracting cudrania tricuspidata polysaccharides and flavones |
| CN102250195A (en) * | 2011-07-05 | 2011-11-23 | 杨柏珍 | Method for producing xanthoceraside |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101537052A (en) * | 2009-04-24 | 2009-09-23 | 山东大学 | Hubei caval vine general flavone extract as well as preparation method and application thereof |
| CN101974094A (en) * | 2010-07-26 | 2011-02-16 | 南京泽朗医药科技有限公司 | Method for comprehensively extracting cudrania tricuspidata polysaccharides and flavones |
| CN102250195A (en) * | 2011-07-05 | 2011-11-23 | 杨柏珍 | Method for producing xanthoceraside |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918800A (en) * | 2013-06-04 | 2014-07-16 | 北京农学院 | Application of xanthoceras sorbifolia oil to prepare anti-fatigue blend oil |
| CN103918800B (en) * | 2013-06-04 | 2015-12-09 | 北京农学院 | The application of shinyleaf yellowhorn oil in preparation antifatigue ready-mixed oil |
| CN104473157A (en) * | 2014-11-25 | 2015-04-01 | 马绍两 | Application of flavone extract of xanthoceras sorbifolia in sugar-reducing and fat-reducing fields and extraction method of flavone extract |
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Application publication date: 20121121 |