CN104473157A - Application of flavone extract of xanthoceras sorbifolia in sugar-reducing and fat-reducing fields and extraction method of flavone extract - Google Patents
Application of flavone extract of xanthoceras sorbifolia in sugar-reducing and fat-reducing fields and extraction method of flavone extract Download PDFInfo
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Abstract
The invention discloses an application of a flavone extract of xanthoceras sorbifolia in the sugar-reducing and fat-reducing fields and an extraction method of the flavone extract, aims at providing an extraction method of flavone in xanthoceras sorbifolia shells, which is efficient, high in yield, short in time consumption, simple to operate, low in production cost, and low in corrosion on equipment, and an extract with a health function. The flavone can be used in the sugar-reducing and fat-reducing fields. The technical scheme is as follows: drying the xanthoceras sorbifolia shells in an oven to a constant weight, crushing the xanthoceras sorbifolia shells into a powder, adding petroleum ether and de-fatting for three times, and adding the filter residue in ethanol solution with a certain volume; and by taking an ionic liquid as a phase transfer catalyst, reacting for 3-25min at 40-80 DEG C to extract a flavone compound, concentrating the extract to obtain a concentrate, adding equal-mass AB-8 macroporous resin in the concentrate, mixing and uniformly stirring, drying and then loading the flavone compound on the AB-8 resin, washing the flavone compound to colourless, then leaching the flavone compound by the ethanol solution, collecting the eluent, carrying out reduced pressure concentration on the eluent and drying to obtain the product.
Description
Technical field
The invention provides the application of shiny-leaved yellowhorn chromocor extract in hypoglycemic field and extracting method thereof, belong to agro-processing technology, the relevant hypoglycemic field such as health food.
Background technology
Shiny-leaved yellowhorn (Xanthoceras sorbifolia.Bunge), have another name called civil official fruit ancient times, civil official spends, existing name shiny-leaved yellowhorn, belong to Sapindaceae machaka or arbor, be the distinctive woody oleiferous plants crop of northern China, be mainly distributed in the Inner Mongol, Shanxi, Shaanxi, in Gansu, also all there is distribution in Ningxia, Shandong, Hebei, Shenyang.Originate in China, also introduced by countries in the world now.Shiny-leaved yellowhorn is the woody oil crops of the distinctive excellent preciousness of China, in each side such as biodiesel exploitation water and soil conservation green lumber cultivation and plants ' aesthetics fields, has great Development volue.Shiny-leaved yellowhorn adaptability is extremely strong, and cold-resistant, drought resisting, impoverishment tolerant ability are strong.In recent years, due to the industrial nutritive value that it is higher, more and more receive the concern of people, nowadays China plants a large amount of wood of shiny-leaved yellowhorn, research direction mainly concentrates in its wood, seed coat, leaf, carpopodium, shell, kernel, chemical composition mainly based on grease, aliphatic acid, saponin(e, terpene, steroid and cumarin, but is studied less to flavones.And shell was not reasonably utilized as discarded object.Research shows, containing number of chemical compositions such as aliphatic acid saponin(e sterol flavones in shinyleaf yellowhorn fruit shell, flavones is the phenolic compound based on benzochromone ring, mostly be crystal-type solid or amorphous powder, color and structure have certain relation, general in yellow, generally exist with glycoside form at occurring in nature, and owing to combining sugared kind number of locations and the difference of connecting mode, various flavonoid glycoside can be formed.Flavone compound has caused the extensive attention of people, because it is almost present in all green plantss, is many medium-height grass the effective elements of the medicines.Research shows that Flavonoid substances has anti-oxidant and effect that is scavenging free radicals; The anti-ageing antitumor antitumaous effect of protection cardio-cerebrovascular; Many physiological functions such as immunity moderation ability and reducing blood sugar and blood fat.The function of flavone compound is approved by people, therefore flavone compound also extensive use.
Ionic liquid is as a kind of novel green solvent and catalyst, have that liquid state range is wide, soluble end is wide, vapour pressure is zero, good stability, Acidity of Aikalinity is adjustable, product is easily separated and a series of particular advantages such as reusable edible, day by day be subject to people's attention in organic synthesis and the application study in extracting, become one of focus of Green Chemistry research.And microwave is as the novel extraction ancillary technique of one, in extraction field, play important role all the time.Microwave can not only make reaction rate improve several times even thousands of times, and also have simple to operate, accessory substance is few, and productive rate is high, easy purifying and advantages of environment protection, can say that the appearance of microwave brings a qualitative leap.The advantage of set ionic liquid of the present invention and microwave technology, in microwave reactor, take ionic liquid as phase transfer catalyst, extracts chromocor compound.Easy and simple to handle, highly shortened the reaction time, good reaction selectivity, productive rate is also higher, is a kind of environmentally friendly technique.
Summary of the invention
The object of the present invention is to provide a kind of method that environmental friendliness, reaction time are short, chromocor compound is extracted in the simple catalysis of technique, its equipment is simple, the chemical reaction thoroughly application of the shiny-leaved yellowhorn chromocor extract of time-saving energy-saving in hypoglycemic field and extracting method thereof again.
The present invention is realized by following scheme: the present invention discloses the application of a kind of shiny-leaved yellowhorn chromocor extract in hypoglycemic field and extracting method thereof, and the method comprises the steps: successively
1) shinyleaf yellowhorn shell is dried to constant weight in baking oven, pulverizes to obtain powder, in the ratio benzinum of 1:10 by shinyleaf yellowhorn shell powder degreasing 15min, and centrifuging and taking lower sediment, dry;
2) in microwave reactor, with the shinyleaf yellowhorn shell powder after degreasing for raw material, take ionic liquid as phase transfer catalyst, ethanol is solvent, be placed in the microwave reactor of condensation reflux unit and infrared probe and react, at 40 ~ 80 DEG C, reaction 3min ~ 25min obtains flavone extractive;
3) obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying;
4) shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 0.6%-1.2% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
Further, the application of above-mentioned shiny-leaved yellowhorn chromocor extract in hypoglycemic field and extracting method thereof, described ionic liquid is bromo 1-alkyl-3-methylimidazole, 1-alkyl-3-methyl imidazolium tetrafluoroborate, 1-alkyl-3-methylimidazole perfluoro octyl sulfonic acid salt, 1-alkyl-3-methylimidazole hexafluorophosphate.
Further, the application of above-mentioned shiny-leaved yellowhorn chromocor extract in hypoglycemic field and extracting method thereof, the alkyl in described ionic liquid used is ethyl, isopropyl, butyl, amyl group, hexyl, heptyl, octyl group, dodecyl.
Further, the application of above-mentioned shiny-leaved yellowhorn chromocor extract in hypoglycemic field and extracting method thereof, the described mass ratio adding ionic-liquid catalyst quality and shiny-leaved yellowhorn benevolence is 1 ~ 16:100, and at 40 ~ 80 DEG C, reaction 3min ~ 25min obtains flavone extractive;
Further, the application of above-mentioned shiny-leaved yellowhorn chromocor extract in hypoglycemic field and extracting method thereof, every gram of described shinyleaf yellowhorn shell powder adds 3 ~ 30 milliliters of alcohol solvents.
Compared with prior art, technical scheme provided by the invention has following technological merit: employing ionic liquid is catalyst, is applied to by microwave radiation preparation in shinyleaf yellowhorn shell in extracting flavonoids, avoids high temperature to the destruction of active ingredient; Decrease the time of extraction, and present higher extraction efficiency, utilize enrichment and the purifying of AB-8 macroreticular resin, the purity of the general flavone of extraction can be made to reach more than 60%, the fields such as food, health products, medicine, cosmetics can be directly applied to.
Detailed description of the invention
Below in conjunction with detailed description of the invention, claim of the present invention is described in further detail; but do not form any limitation of the invention; the amendment of anyone limited number of time made within the scope of the claims in the present invention, still within claims of the present invention.
Embodiment 1
Get the shiny-leaved yellowhorn powder 10g after pulverizing, add 100ml benzinum by shinyleaf yellowhorn shell powder degreasing 15min, centrifuging and taking lower sediment, dry; Take the shinyleaf yellowhorn shell powder 5g after drying defatted respectively, ethanolic solution 30ml, ionic liquid bromo 1-isopropyl-3-methylimidazole 0.5g, adds in the round-bottomed flask with agitator, thermometer, reflux condensing tube, magnetic agitation, reacts 15min and obtains flavone extractive at 45 DEG C; Obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying.In product, general flavone yield is 3.23%, and general flavone purity is 61.43%.Shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 0.6% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
Embodiment 2
Get the shiny-leaved yellowhorn powder 10g after pulverizing, add 100ml benzinum by shinyleaf yellowhorn shell powder degreasing 15min, centrifuging and taking lower sediment, dry; Take the shinyleaf yellowhorn shell powder 5g after drying defatted respectively, ethanolic solution 30ml, ionic liquid bromo 1-ethyl-3-methylimidazole 0.4g, adds in the round-bottomed flask with agitator, thermometer, reflux condensing tube, magnetic agitation, reacts 10min and obtains flavone extractive at 60 DEG C; Obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying.In product, general flavone yield is 3.52%, and general flavone purity is 60.43%.Shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 0.8% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
Embodiment 3
Get the shiny-leaved yellowhorn powder 10g after pulverizing, add 100ml benzinum by shinyleaf yellowhorn shell powder degreasing 15min, centrifuging and taking lower sediment, dry; Take the shinyleaf yellowhorn shell powder 5g after drying defatted respectively, ethanolic solution 30ml, ionic liquid bromo 1-hexyl-3-methylimidazole 0.3g, adds in the round-bottomed flask with agitator, thermometer, reflux condensing tube, magnetic agitation, reacts 14min and obtains flavone extractive at 65 DEG C; Obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying.In product, general flavone yield is 4.22%, and general flavone purity is 60.78%.Shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 0.9% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
Embodiment 4
Get the shiny-leaved yellowhorn powder 10g after pulverizing, add 100ml benzinum by shinyleaf yellowhorn shell powder degreasing 15min, centrifuging and taking lower sediment, dry; Take the shinyleaf yellowhorn shell powder 5g after drying defatted respectively, ethanolic solution 30ml, ionic liquid bromine 1-butyl-3-methylimidazole hexafluorophosphate 0.1g, adds in the round-bottomed flask with agitator, thermometer, reflux condensing tube, magnetic agitation, reacts 8min and obtains flavone extractive at 65 DEG C; Obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying.In product, general flavone yield is 3.12%, and general flavone purity is 60.49%.Shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 1.0% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
Embodiment 5
Get the shiny-leaved yellowhorn powder 10g after pulverizing, add 100ml benzinum by shinyleaf yellowhorn shell powder degreasing 15min, centrifuging and taking lower sediment, dry; Take the shinyleaf yellowhorn shell powder 5g after drying defatted respectively, ethanolic solution 30ml, ionic liquid bromo 1-heptyl-3-methylimidazole 0.6g, adds in the round-bottomed flask with agitator, thermometer, reflux condensing tube, magnetic agitation, reacts 12min and obtains flavone extractive at 70 DEG C; Obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying.In product, general flavone yield is 4.12%, and general flavone purity is 60.59%.Shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 1.1% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
Claims (5)
1. the application of shiny-leaved yellowhorn chromocor extract in hypoglycemic field and an extracting method thereof, is characterized in that adopting following steps:
1) shinyleaf yellowhorn shell is dried to constant weight in baking oven, pulverizes to obtain powder, in the ratio benzinum of 1:10 by shinyleaf yellowhorn shell powder degreasing 15min, and centrifuging and taking lower sediment, dry;
2) in microwave reactor, with the shinyleaf yellowhorn shell powder after degreasing for raw material, take ionic liquid as phase transfer catalyst, ethanol is solvent, and at 40 ~ 80 DEG C, reaction 3min ~ 25min obtains flavone extractive;
3) obtain concentrate after extract is concentrated, the AB-8 macroreticular resin quality such as adding in concentrate mix and stirs, and goes up AB-8 resin after dry, be washed to colourless, again with ethanolic solution drip washing, collect eluent, namely eluent reduced pressure concentration also obtains product after drying;
4) shiny-leaved yellowhorn flavones is in the application of functions of reducing sugar and reducing fat field of food: join in food in the ratio of 0.6%-1.2% (mass ratio), food comprises bread and cheese and health food, the products such as optional capsule, pulvis.
2. the application of shiny-leaved yellowhorn chromocor extract as claimed in claim 1 in hypoglycemic field and extracting method thereof, it is characterized in that, described ionic liquid is bromo 1-alkyl-3-methylimidazole, 1-alkyl-3-methyl imidazolium tetrafluoroborate, 1-alkyl-3-methylimidazole perfluoro octyl sulfonic acid salt, 1-alkyl-3-methylimidazole hexafluorophosphate.
3. method as claimed in claim 2, is characterized in that: the alkyl in ionic liquid used is ethyl, isopropyl, butyl, amyl group, hexyl, heptyl, octyl group, dodecyl.
4. the application of shiny-leaved yellowhorn chromocor extract as claimed in claim 1 in hypoglycemic field and extracting method thereof, it is characterized in that, the described mass ratio adding ionic-liquid catalyst quality and shiny-leaved yellowhorn benevolence is 1 ~ 16:100, and at 40 ~ 80 DEG C, reaction 3min ~ 25min obtains flavone extractive.
5. the application of shiny-leaved yellowhorn chromocor extract as claimed in claim 1 in hypoglycemic field and extracting method thereof, it is characterized in that, every gram of described shinyleaf yellowhorn shell powder adds 3 ~ 30 milliliters of alcohol solvents.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106177101A (en) * | 2015-04-29 | 2016-12-07 | 甘肃长瑞文冠果产业发展有限公司 | The new application at middle mongolian medicine shinyleaf yellowhorn fruit shell position |
| CN112042845A (en) * | 2020-10-15 | 2020-12-08 | 松林 | Xanthoceras sorbifolia leaf beverage and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350001A (en) * | 2001-10-31 | 2002-05-22 | 杨柏珍 | Fruit shell capable of being used to extract shinyleaf yellowhorn general saponin, coarse fat, coarse protein and sugar |
| CN102783656A (en) * | 2012-09-04 | 2012-11-21 | 山东理工大学 | Application of shiny-leaved yellowhorn flavone in lipid-lowering weight-losing field and preparation method of shiny-leaved yellowhorn flavone |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350001A (en) * | 2001-10-31 | 2002-05-22 | 杨柏珍 | Fruit shell capable of being used to extract shinyleaf yellowhorn general saponin, coarse fat, coarse protein and sugar |
| CN102783656A (en) * | 2012-09-04 | 2012-11-21 | 山东理工大学 | Application of shiny-leaved yellowhorn flavone in lipid-lowering weight-losing field and preparation method of shiny-leaved yellowhorn flavone |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106177101A (en) * | 2015-04-29 | 2016-12-07 | 甘肃长瑞文冠果产业发展有限公司 | The new application at middle mongolian medicine shinyleaf yellowhorn fruit shell position |
| CN112042845A (en) * | 2020-10-15 | 2020-12-08 | 松林 | Xanthoceras sorbifolia leaf beverage and preparation method thereof |
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