CN102813214A - Application of xanthoceras sorbifolia saponin in field of reducing lipid and losing weight and preparation method of xanthoceras sorbifolia saponin - Google Patents
Application of xanthoceras sorbifolia saponin in field of reducing lipid and losing weight and preparation method of xanthoceras sorbifolia saponin Download PDFInfo
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- CN102813214A CN102813214A CN2012103214056A CN201210321405A CN102813214A CN 102813214 A CN102813214 A CN 102813214A CN 2012103214056 A CN2012103214056 A CN 2012103214056A CN 201210321405 A CN201210321405 A CN 201210321405A CN 102813214 A CN102813214 A CN 102813214A
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Abstract
The invention aims to provide a method for preparing saponin from xanthoceras sorbifolia shells with high efficiency, high purity, high yield, short time consumption, simplicity in operation, low production cost and low equipment corrosion and xanthoceras sorbifolia saponin with efficient lipid reducing and weight losing effects. The saponin can be applied in the field of reducing lipid and losing weight, for example, the saponin is added into functional food in a certain ratio, and xanthoceras sorbifolia saponin functional food with a good effect can be obtained. According to the technical scheme, the method comprises the following steps of: extracting the crushed and degreased xanthoceras sorbifolia shells by using a certain quantity of solution, filtering, concentrating, extracting with ethyl acetate so as to remove impurities, extracting with saturated n-butanol, concentrating, filling into a column for chromatography, collecting a part of eluent, and concentrating and freeze-drying the eluent, thus obtaining the xanthoceras sorbifolia saponin. By adding the xanthoceras sorbifolia saponin into the food in a certain ratio, effects of reducing lipid and losing weight can be achieved.
Description
Technical field
The present invention provides bunkankasaponin application in the lowering blood-fat and reducing weight field and preparation method thereof, belongs to agro-processing technology, relevant lowering blood-fat and reducing weight such as functional food field.
Background technology
Shiny-leaved yellowhorn belongs to the Sapindaceae shiny-leaved yellowhorn and belongs to, and also is referred to as civil official fruit, xanthoceras sobifolia bunge etc., is the woody seeds of the distinctive preciousness of China.It is very high that shinyleaf yellowhorn shell contains the saponin amount, and colorimetric estimation reaches 4%.Bunkankasaponin belongs to the oleanane type saponin, is prevalent in the plants such as pulse family, Araliaceae, Carrot family, Primulaceae, Curcurbitaceae.Oside compound aboundresources and be easy to extract extensively exists in organs such as the stem of plant, leaf, flower, fruit, be one group at structure and different in nature materials, the platymiscium secondary metabolite.The shinyleaf yellowhorn shell saponin has the effect of anti-anoxic, anti-oxidant, antifatigue.
Oside compound adopts solvent extraction usually.Mixed solvent such as methanol-water that the general available water of saponin, methyl alcohol, ethanol, acetone or polarity are bigger extract.Though technology is stablized feasible, efficient is low, length consuming time, and the reagent consumption is big, and solvent is prone to residual, and environment is had certain infringement.Pancreatic lipase is to absorb fatty most important enzyme in the digestive system; Food fat decomposes under the effect of pancreatic lipase in digesting and assimilating process, generates aliphatic acid and 2-acylglycerol; These steatolysis products mix with bile salt, and dispersion could be by little intestinal absorption after forming particulate.If can suppress the pancreatic lipase activity, just can postpone and reduce the absorption of small intestine, thereby reach anti-fat effect for reducing fat food fat.Research shows that plant sapogenin glycoside can make the absorption of fat reduce through suppressing the pancreatic lipase activity, thereby reaches the anti-fat effect of lipopenicillinase.For example panax quinquefolium saponin is 90% to the active inhibiting rate of pancreatic lipase, and panaxoside reaches 96%-100% to the pancreatic lipase maximum inhibition, and total saponin from platycodon grandiflorum reaches 41.7% to pancreatic lipase pancreatic lipase maximum inhibition.Pancreatic lipase is the most important enzyme of hydrolysis meals class fat, and isoelectric point is 5.0 acidic protein molecule.The food fat of hydrolyzable 50% ~ 70% is so can suppress high fat of blood through suppressing the pancreatic lipase activity.Yet, be that the Weight-reducing and lipid-lowering food of composition does not have report as yet at present with the bunkankasaponin.
Summary of the invention
The purpose of this invention is to provide a kind of efficient, purity is high, yield is high, weak point consuming time, simple to operate, production cost is low, to saponin preparation method in the low shinyleaf yellowhorn shell of equipment corrosion and have efficient lowering blood-fat and reducing weight effect bunkankasaponin; This saponin can be used for the lowering blood-fat and reducing weight field; For example join by a certain percentage in the functional food, can obtain respond well bunkankasaponin functional food field.
Its technical scheme is: bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof; It is characterized in that adopting following steps: (1) shinyleaf yellowhorn fruit shell is pulverized; Use the solution of the volume of 20%-40% as 10-20 times of milliliter of shinyleaf yellowhorn shell opaque amount gram number; 70-75 ℃ of following lixiviate 120～240 minutes filtered; (2) it is subsequent use filtrating to be condensed into paste, after the ethyl acetate extraction removal of impurities, uses water saturated extracting n-butyl alcohol, concentrate medicinal extract; (3) medicinal extract is crossed column chromatography, collect eluent, eluent is concentrated freeze-dried, promptly get bunkankasaponin; (4) bunkankasaponin is in the application in lowering blood-fat and reducing weight functional food field: the ratio in 0.5%-1.1% (mass ratio) joins in the food, and food comprises bread and cheese and health food, products such as optional capsule, pulvis.
Described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and shinyleaf yellowhorn shell is pulverized order and counted the 5-20 order in the step 1, and shinyleaf yellowhorn shell needs degreasing degreasing.
Described bunkankasaponin contains 5%-10% methyl alcohol in the application in lowering blood-fat and reducing weight field and preparation method thereof in the used solution in the step 1.
Described bunkankasaponin concentrates in the step 2 and can adopt decompression to concentrate in the application in lowering blood-fat and reducing weight field and preparation method thereof; The decompression thickening temperature is 60～70 ℃, under 0.1～0.7 atmospheric pressure; Also can adopt the normal pressure heating.
Described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and used filler is the AB-8 macroreticular resin in the step 3, and eluent is 75% ethanol.
Described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, wants mixing and stirring after adding functional food in the step 4 to.
Described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and this functional food lowering blood-fat and reducing weight mode is to be approach to suppress the pancreatic lipase activity in the step 4.
The present invention compared with prior art, the mixed solution that the technology that is adopted has used ethanol and methyl alcohol to form has reduced the consumption of methyl alcohol, has reduced dissolvent residual, has improved the extraction performance of solvent, has improved the recovery rate of saponin.
Compare with traditional handicraft, technology is simple, and recovery rate is high (to be compared with simple ethanol extraction; Recovery rate has increased about 20%), yield high (compare with simple ethanol extraction, yield has improved about 13%); Methanol usage significantly reduces (under the identical recovery rate, than simple methyl alcohol extraction, methanol usage has reduced about 42%); Production process is simple, and is easy to operate, is prone to industrialization, mechanization, automated production.And the bunkankasaponin that the method obtains is active through suppressing pancreatic lipase, can realize functions of lowering blood-fat and reducing weight preferably.Bunkankasaponin is through testing discovery to active inhibition of pancreatic lipase, and bunkankasaponin can suppress the pancreatic lipase activity and reach 50%, proves tangible functions of lowering blood-fat and reducing weight.
Preferred forms embodiment 1: shinyleaf yellowhorn fruit shell is crushed to the 5-20 order after the degreasing, and with ethanol content 20%, 500 milliliters of the solution of methanol content 10% soak 50g shinyleaf yellowhorn shell powder, and 70 ℃ of following lixiviates 120 minutes are filtered; (2) will filtrate under the normal pressure after boiling removes about many milliliters of ethanol, water and methyl alcohol to 150; Change decompression into and concentrate, it is 70 ℃ that decompression concentrates condition, 0.1 atmospheric pressure; After 100 milliliters of ethyl acetate extraction removal of impurities; Water saturated n-butanol with 2 times of volumes divides 3 extractions again, collects the n-butanol phase, concentrates; (3) bunkankasaponin medicinal extract is added on the column chromatography filler, uses 75% ethanol elution then, collect the part eluent; (4) bunkankasaponin is in the application in lowering blood-fat and reducing weight functional food field: bunkankasaponin is joined mixing and stirring in the ground rice in the ratio of 0.5% (mass ratio), can suppress the pancreatic lipase activity and reach 45%.
Embodiment 2: shinyleaf yellowhorn fruit shell is crushed to the 5-20 order after the degreasing, and with ethanol content 40%, 1000 milliliters of the solution of methanol content 5% soak 50g shinyleaf yellowhorn shell powder, and 70 ℃ of following lixiviates 240 minutes are filtered; (2) will filtrate under the normal pressure after boiling removes about many milliliters of ethanol, water and methyl alcohol to 200, and changing decompression into and concentrating, it is 70 ℃ that decompression concentrates condition, 0.1 atmospheric pressure.Be condensed into postlyophilization; (3) bunkankasaponin medicinal extract is added on the column chromatography filler, uses 75% ethanol elution then, collect the part eluent; (4) bunkankasaponin is in the application in lowering blood-fat and reducing weight field: bunkankasaponin is joined mixing and stirring in the bread and cheese in the ratio of 1.1% (mass ratio), can suppress the pancreatic lipase activity and reach 58 %.
Claims (7)
1. bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof; It is characterized in that adopting following steps: (1) shinyleaf yellowhorn fruit shell is pulverized; The volume that uses 20%-40% is the shinyleaf yellowhorn shell opaque amount gram number 10-20 solution of milliliter doubly, and 70-75 ℃ of following lixiviate 120～240 minutes filtered; (2) it is subsequent use filtrating to be condensed into paste, after the ethyl acetate extraction removal of impurities, uses water saturated extracting n-butyl alcohol, concentrate medicinal extract; (3) medicinal extract is crossed column chromatography, collect eluent, eluent is concentrated freeze-dried, promptly get bunkankasaponin; (4) bunkankasaponin is in the application in lowering blood-fat and reducing weight functional food field: the ratio in 0.5%-1.1% (mass ratio) joins in the food, and food comprises bread and cheese and health food, products such as optional capsule, pulvis.
2. described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and shinyleaf yellowhorn shell is pulverized order and counted the 5-20 order in the step 1, and shinyleaf yellowhorn shell needs degreasing degreasing.
3. described bunkankasaponin contains 5%-10% methyl alcohol in the application in lowering blood-fat and reducing weight field and preparation method thereof in the used solution in the step 1.
4. described bunkankasaponin concentrates in the step 2 and can adopt decompression to concentrate in the application in lowering blood-fat and reducing weight field and preparation method thereof; The decompression thickening temperature is 60～70 ℃, under 0.1～0.7 atmospheric pressure; Also can adopt the normal pressure heating.
5. described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and used filler is the AB-8 macroreticular resin in the step 3, and eluent is 75% ethanol.
6. described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, wants mixing and stirring after adding functional food in the step 4 to.
7. described bunkankasaponin is in the application in lowering blood-fat and reducing weight field and preparation method thereof, and this functional food lowering blood-fat and reducing weight mode is to be approach to suppress the pancreatic lipase activity in the step 4.
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| CN2012103214056A CN102813214A (en) | 2012-09-04 | 2012-09-04 | Application of xanthoceras sorbifolia saponin in field of reducing lipid and losing weight and preparation method of xanthoceras sorbifolia saponin |
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| CN2012103214056A CN102813214A (en) | 2012-09-04 | 2012-09-04 | Application of xanthoceras sorbifolia saponin in field of reducing lipid and losing weight and preparation method of xanthoceras sorbifolia saponin |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350001A (en) * | 2001-10-31 | 2002-05-22 | 杨柏珍 | Can be used for extracting xanthoceras sorbifolia Bunge total saponin, crude fat, crude protein, and sugar |
| CN1626545A (en) * | 2004-07-07 | 2005-06-15 | 杨柏珍 | Compound extracted from xanthoceras sorbifolia shells and stems, extraction method and application |
| CN102250195A (en) * | 2011-07-05 | 2011-11-23 | 杨柏珍 | Method for producing xanthoceraside |
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- 2012-09-04 CN CN2012103214056A patent/CN102813214A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1350001A (en) * | 2001-10-31 | 2002-05-22 | 杨柏珍 | Can be used for extracting xanthoceras sorbifolia Bunge total saponin, crude fat, crude protein, and sugar |
| CN1626545A (en) * | 2004-07-07 | 2005-06-15 | 杨柏珍 | Compound extracted from xanthoceras sorbifolia shells and stems, extraction method and application |
| CN102250195A (en) * | 2011-07-05 | 2011-11-23 | 杨柏珍 | Method for producing xanthoceraside |
Non-Patent Citations (5)
| Title |
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| 《第二次世界中西医结合大会论文摘要集》 20021231 郑毅男 西洋参皂甙的分离及其抑制胰脂肪酶活性的研究 第497页第1-8行 , * |
| 《食品科学》 20091231 李光勋等 正交试验优选文冠果壳中文冠果壳苷提取工艺 59页左栏第4行-60页左栏16行 第30卷, 第2期 * |
| 李光勋等: "正交试验优选文冠果壳中文冠果壳苷提取工艺", 《食品科学》, vol. 30, no. 2, 31 December 2009 (2009-12-31) * |
| 王先科等: "植物皂甙降低机体胆固醇的作用及其机理研究进展", 《江西农业学报》, vol. 22, no. 1, 31 December 2010 (2010-12-31), pages 132 - 135 * |
| 郑毅男: "西洋参皂甙的分离及其抑制胰脂肪酶活性的研究", 《第二次世界中西医结合大会论文摘要集》, 31 December 2002 (2002-12-31) * |
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