CN102766533B - 一种水酶法提取大豆油脂生物酶破乳的方法 - Google Patents
一种水酶法提取大豆油脂生物酶破乳的方法 Download PDFInfo
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Abstract
Description
大豆 | 黑龙江农业科学院培植的垦农42,其中蛋白含量为41.6%,油脂含量为21.3%,含水率为10.6%,灰分4.3%。 |
Alcalase碱性内切蛋白酶 | novo公司(1.2×105U/mL) |
仪器 | 生产厂家 |
CX-500 型超声波清洗机 | 北京医疗设备二厂 |
电子分析天平 | 梅特勒-托利多仪器(上海)有限公司 |
锤片式粉碎机 | 中国天津泰斯特仪器有限公司 |
LDZ5-2型台式低速离心机 | 上海安亭科学仪器厂 |
电热恒温水浴锅 | 余姚市东方电工仪器厂 |
TGL-16G高速台式离心机 | 上海安亭科学仪器厂 |
精密电动搅拌机 | 江苏省金坛市荣华仪器 |
索氏抽提器 | 天津玻璃仪器厂 |
电热恒温鼓风干燥箱 | 上海一恒科学仪器有限公司 |
大豆 | 黑龙江农业科学院培植的垦农42,其中蛋白含量为41.6%,油脂含量为21.3%,含水率为10.6%,灰分4.3%。 |
仪器 | 生产厂家 |
CX-500 型超声波清洗机 | 北京医疗设备二厂 |
电子分析天平 | 梅特勒-托利多仪器(上海)有限公司 |
锤片式粉碎机 | 中国天津泰斯特仪器有限公司 |
LDZ5-2型台式低速离心机 | 上海安亭科学仪器厂 |
电热恒温水浴锅 | 余姚市东方电工仪器厂 |
TGL-16G高速台式离心机 | 上海安亭科学仪器厂 |
精密电动搅拌机 | 江苏省金坛市荣华仪器 |
索氏抽提器 | 天津玻璃仪器厂 |
电热恒温鼓风干燥箱 | 上海一恒科学仪器有限公司 |
超声细胞破碎机 | 宁波新芝生物科技股份有限公司 |
变量 | 自由度 | 平方和 | 均方 | F值 | Pr>F |
x 1 | 1 | 164.4094 | 164.4094 | 141.0005 | 0.0001 |
x 2 | 1 | 362.673 | 362.673 | 311.0349 | 0.0001 |
x 3 | 1 | 30.04436 | 30.04436 | 25.76659 | 0.0005 |
x 1 2 | 1 | 233.4168 | 233.4168 | 200.1825 | 0.0001 |
x 1 x2 | 1 | 8.20125 | 8.20125 | 7.03354 | 0.0242 |
x 1 x3 | 1 | 33.53805 | 33.53805 | 28.76284 | 0.0003 |
x 2 2 | 1 | 195.7064 | 195.7064 | 167.8413 | 0.0001 |
x 2x3 | 1 | 60.5 | 60.5 | 51.8859 | 0.0001 |
x 3 2 | 1 | 146.8259 | 146.8259 | 125.9206 | 0.0001 |
回归 | 9 | 1141.8259 | 126.8091 | 108.7538 | 0.0001 |
剩余 | 10 | 11.6602 | 1.16602 | ||
失拟 | 5 | 1.973652 | 0.39473 | 0.203752 | 0.9472 |
总和 | 19 | 1152.942 |
因素 | 水平值 | 实际值 |
超声温度 | 0.39227 | 71.9614 |
超声时间 | 0.72154 | 3.7215 |
料液比 | 0.20429 | 2.1021 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105349246A (zh) * | 2015-11-12 | 2016-02-24 | 东北农业大学 | 一种从文冠果中同步提取油脂及蛋白肽的方法 |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102911781A (zh) * | 2012-11-12 | 2013-02-06 | 东北农业大学 | 一种水酶法结合亚临界萃取大豆油脂的方法 |
CN102911782A (zh) * | 2012-11-13 | 2013-02-06 | 东北农业大学 | 一种水酶法提取大豆油脂破乳的方法 |
CN102994209B (zh) * | 2012-12-17 | 2014-07-09 | 东北农业大学 | 一种水酶法同步提取大豆油脂与大豆蛋白的方法 |
CN104893817B (zh) * | 2015-06-03 | 2018-08-17 | 东北农业大学 | 一种水酶法提取大豆油复合破乳的方法 |
CN106986917A (zh) * | 2017-04-12 | 2017-07-28 | 岭南师范学院 | 一种从茶油油层中分离蛋白质的方法 |
CN108641804B (zh) * | 2018-04-13 | 2021-12-31 | 华侨大学 | 一种超声辅助水酶法提取黄秋葵籽油和蛋白质的方法 |
CN108690704A (zh) * | 2018-05-31 | 2018-10-23 | 镇江虎瑞生物科技有限公司 | 一种微波法制备菜籽油的工艺 |
CN113046170B (zh) * | 2021-03-26 | 2022-03-11 | 大连奥川生物科技有限公司 | 富含呋喃类脂不皂化物的鳄梨油制备方法和应用 |
Citations (3)
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CN1632127A (zh) * | 2003-12-25 | 2005-06-29 | 黑龙江省大豆技术开发研究中心 | 一种酶改性大豆磷脂的生产方法 |
CN101602979A (zh) * | 2009-06-09 | 2009-12-16 | 东北农业大学 | 一种大豆油脂的提取方法 |
CN101974364A (zh) * | 2009-09-27 | 2011-02-16 | 东北农业大学 | 一种从大豆乳状液中提取大豆油脂的方法 |
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CN1632127A (zh) * | 2003-12-25 | 2005-06-29 | 黑龙江省大豆技术开发研究中心 | 一种酶改性大豆磷脂的生产方法 |
CN101602979A (zh) * | 2009-06-09 | 2009-12-16 | 东北农业大学 | 一种大豆油脂的提取方法 |
CN101974364A (zh) * | 2009-09-27 | 2011-02-16 | 东北农业大学 | 一种从大豆乳状液中提取大豆油脂的方法 |
Non-Patent Citations (2)
Title |
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热处理对大豆乳状液破乳工艺的研究;王文睿等;《食品工业科技》;20120229(第4期);285-287 * |
王文睿等.热处理对大豆乳状液破乳工艺的研究.《食品工业科技》.2012,(第4期),285-287. |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105349246A (zh) * | 2015-11-12 | 2016-02-24 | 东北农业大学 | 一种从文冠果中同步提取油脂及蛋白肽的方法 |
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