CN102757918A

CN102757918A - Thermophilic bacillus and application thereof

Info

Publication number: CN102757918A
Application number: CN2012102555956A
Authority: CN
Inventors: 崔荣
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-10-31
Filing date: 2012-07-24
Publication date: 2012-10-31
Anticipated expiration: 2032-07-24
Also published as: CN102757918B

Abstract

The invention relates to a novel microbe, and in particular relates to a thermophilic bacillus and an application thereof. The strain was conserved on Oct 27, 2011, the conservation number is CCTCCM2011366, and the conservation authority is the China Center for Type Culture Collection. The strain belongs to Geobacillus category, and can be reproduced at the temperature of 80 DEG C above. According to the invention, organic matters can be efficiently and rapidly decomposed into carbon dioxide and water harmless to the environment. The thermophilic bacillus has great potential in the application of garbage treatment and the like.

Description

A kind of bacillus acidocldarius and uses thereof

Technical field

The present invention relates to a kind of novel microorganism, specifically a kind of bacillus acidocldarius and uses thereof.

Background technology

As everyone knows; In daily life; Inevitably produce rubbish, in case and long storage time such as the chicken bone that produces in the kitchen, dish leaf, dish stalk, leftovers, pericarp, eggshell just rot easily; Emit bad smell and breed bacteria, people need at short notice food waste to be handled for this reason.And present stage mainly be to be transported to the Refuse bin to abandon or let alone to enter water drain and wash away to the processing mode of food waste, no matter adopt existing which kind of mode, all can't avoid outside rubbish is exposed to once more the secondary pollution that causes.This obviously can't thoroughly solve the handling problem of food waste.

Summary of the invention

The present invention proposes a kind of bacillus acidocldarius and uses thereof, can effectively solve the shortcoming and defect that exists in the prior art.

The present invention reaches through following measure:

A kind of bacillus acidocldarius; It is characterized in that this bacterial strain is that preservation date is on October 27th, 2011; Preserving number is the bacterial strain of CCTCC NO:M2011366; Its depositary institution is Chinese typical culture collection center, and this bacterial strain belongs to Geobacillus and belongs to, and its Latin formal name used at school is Geobacillus thermoglucosidasius ZHENG8.

Bacillus acidocldarius described in the present invention is from the soil of Weihai City, China Shandong Province, to separate to obtain, and the temperature condition during separation is more than 50 ℃, and this bacterial strain can be in growth more than 80 ℃; Grow down and breed and enliven at 50-60 ℃; This bacterial strain is a gram positive organism, good salt, good acid, thermophilic bacterium, in addition; It resolves into CO2 and water to organic waste in the time of 50-60 ℃, rate of decomposition can reach more than 90%.

The bacillus acidocldarius that the invention also discloses preserving number and be CCTCC NO:M2011366 is as the purposes in the refuse treatment.

The invention also discloses preserving number is the purposes of the bacillus acidocldarius of CCTCC NO:M2011366 as glycase production.

The invention also discloses preserving number is the purposes of the bacillus acidocldarius of CCTCC NO:M2011366 as proteolytic enzyme production.

The present invention can be decomposed into organism the carbonic acid gas and the water of environmental sound efficiently, fast, in the application of aspects such as refuse treatment, has great potential.

The preservation explanation of said bacterial classification:

The preservation date of said bacterial classification is on October 27th, 2011;

The depositary institution of said bacterial classification is Chinese typical culture collection center, is called for short CCTCC;

The deposit number of said bacterial classification is CCTCC NO:M2011366;

Embodiment:

Below in conjunction with embodiment the present invention is further described.

Bacillus acidocldarius described in the present invention is to separate acquisition more than 50 ℃ from organic soil, and can be in growth more than 80 ℃, and still growth and breeding are active under 50-60 ℃; This bacterial strain is a gram positive organism, good salt, good acid; Thermophilic bacterium; Through discovering, it resolves into CO2 and water to organic waste in the time of 50-60 ℃, and rate of decomposition can reach more than 90%.

A kind of bacillus acidocldarius described in the present invention, thermophilic specifically glucose glucosides ground bacillus, its Latin name is called Geobacillus thermoglucosidasius ZHENG8, and whole base sequences of its 16SrDNA are shown in sequence table 1.

A kind of bacillus acidocldarius proposed by the invention is a kind of new thermophilic acidophilic acid halophilism bacterium that under condition more than 50 ℃, is obtained from soil; The present inventor is attempting the thermophilic microorganism that the soil around the Residential areas exists under temperature more than 50 ℃ is retrieved; The result found 30-40 ℃ of common microbial culture temperature down increment slowly, and 60-70 ℃ especially under the temperature about 65 ℃ growing multiplication very active; The present inventor has carried out the phylogenetic systematics analysis based on the base sequence of the 16SrDNA of this bacterium, and the result finds that it is close that this bacterium belongs to gram-positive soil bacteria bacillus Geobacillus, is bacterium independently on the genus level at least.

The present inventor is with its called after HyenaA bacterium.

Bacterium of the present invention separates acquisition from the soil of Residential areas, Weihai; The concrete separation method of this bacterial strain is: in 10 milliliters of nutrient solutions, add the above-mentioned soil of about 0.1 gram; 50 ℃ keep using the Carnis Bovis seu Bubali cream substratum, the Congo red substratum of Mierocrystalline cellulose, casein substratum under the temperature; Separate repeatedly and purifying in the starch culture-medium, obtain aimed strain; Said substratum is prepared by following weight proportion by following each component:

Carnis Bovis seu Bubali cream 5g;

Albumin glue 10g;

Sodium-chlor 5g;

Agar 20g;

Water 1000ml;

The pH value of said substratum is 7.0.

Microbiological property and the taxonomy of gained bacterium are described below, the character of bacterial classification: Gram-positive, and bacillus, aerobic; Produce gemma, optimum growth temperature 60-70 ℃, bacterium colony is circular on the LB substratum, and colony diameter is about 1.0-2.0 ㎜; Oyster white, smooth surface is opaque.

This bacterium is 60-70 ℃ of following active growth, but in not breeding below 10 ℃; It breeds active under the high temperature more than 65 ℃, even if propagation is still arranged under 80 ℃; The optimal pH of propagation is acid, and the PH scope that can breed is 5-7, and in addition, it has stronger halophilism; Various albumen such as BSA and casein and starch there is resolving power.

According to the above content, judge that mikrobe of the present invention belongs to eubacterium, be thermophilic acidophilic acid halophilism bacterium; In addition; According to the base sequence of 16SRDHA in the sequence table, judge it and the nearly edge of GGeobacillus thermoglucosidasius, belong to but form independently.

The bacillus acidocldarius that the invention also discloses preserving number and be CCTCC NO:M2011366 is further described the present invention below in conjunction with embodiment as the purposes in the refuse treatment:

Embodiment 1:

In processing vessel, drop into dish leaf 2.5kg; Add and contain wood powder of this mikrobe and the mixture 1000g of mikrobe, wherein the mass percent of mikrobe in mixture is 20%, behind the material thorough mixing in the container; Make it under 60 ℃ condition; React after 12 hours, reaction result is for fully to be decomposed into CO2 and water with the dish leaf, and rate of decomposition is 96%.

Embodiment 2:

The surplus soup 2kg of leftovers that in processing vessel, adds common dining room; In container, add this mikrobe then; Make the content of this mikrobe in the said mixture remain on 25%-30%, after reacting 12 hours under 60 ℃ the condition, residue in the inspect vessel; The surplus soup of leftovers is broken down into CO2 and water, and rate of decomposition is 90%.

Embodiment 3:

In processing vessel, add steamed bun 2kg; In container, add the liquid 1000ml that contains this mikrobe then; Said liquid is the mixture of substratum and this mikrobe, the mixture in the processing vessel is reacted 8 hours, residue in the detection receptacle under 60 ℃ condition; The steamed bun that drops into is broken down into CO2 and water, and rate of decomposition is 95%.

Embodiment 4:

In processing vessel, add rice 2kg; In container, add the liquid 1000ml that contains this mikrobe then; Said liquid is the mixture of substratum and this mikrobe, the mixture in the processing vessel is reacted 8 hours, residue in the detection receptacle under 50 ℃-60 ℃ condition; The rice that drops into is broken down into CO2 and water, and rate of decomposition is 96%.

Embodiment 5:

In processing vessel, add edible oil 200g, in container, add the liquid 1000ml that contains this mikrobe then, said liquid is the mixture of substratum and this mikrobe; Mixture in the processing vessel was reacted 8 hours under 50 ℃-60 ℃ condition; Residue in the detection receptacle, input edible oil is decomposed more than 80%, and the interior residue of detection receptacle after 24 hours is carried out in reaction; The edible oil that drops into is broken down into CO2 and water, and rate of decomposition is more than 95%.

ACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCGGGCGGAAGC

TTGCTTCCGCTTGGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCT

GCCCGTAAGACCGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAAC

ACCGAAGACCGCATGGTCTTTGGTTGAAAGGCGGCTTCGGCTGCCACTTA

CGGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAA

GGCGACGATGCGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTG

AGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAA

TGGACGAAAGTCTGACGGAGCGACGCCGCGTGAGCGAAGAAGGTCTTCGG

ATCGTAAAGCTCTGTTGTTAGGGAAGAAGAAGTGCCGTTCGAATAGGGCG

GCACGGTGACGGTACCTAACGAGAAAGCCCCGGCTAACTACGTGCCAGCA

GCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAA

AGCGCGCGCAGGCGGTCCCTTAAGTCTGATGTGAAAGCCCACGGCTTAAC

CGTGGAGGGTCATTGGAAACTGGGGGACTTGAGTGCAGAAGAGGAGAGCG

GAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGT

GGCGAAGGCGGCTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTG

GGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG

TGCTAAGTGTTAGAGGGGTTACCCCTTTAGTGCTGTAGCTAACGCGTTAA

GCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGA

CGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCG

AAGAACCTTACCAGGTCTTGACATCCCCTGACAACCCTGGAGACAGGGCG

TTCCTCCCTTCGGGAGGACAGGGTGACAGGTGGTGCATGGTTGTCGTCAG

CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGC

CCCTAGTTGCCAGCATTCAGTTGGGCACTCTAGGGGGACTGCCGGTGACA

AACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCT

GGGCTACACACGTGCTACAATGGGCGGTACAAAGGGCTGCGAACCCGCGA

GGGGGAGCGAATCCCAAAAAGCCGCTCTCAGTTCGGATTGCAGGCTGCAA

CTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGC

GGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAG

CTTGCAACACCCGAAGTCGGTGAGGTAACCCGCAAGGGAGCCAGCCGCCG

AAGGTGGGGCAAGTGATTGGG

Claims

1. bacillus acidocldarius; It is characterized in that this bacterial strain is that preservation date is on October 27th, 2011; Preserving number is the bacterial strain of CCTCC M 2011366, and its depositary institution is Chinese typical culture collection center, and this bacterial strain belongs to Geobacillus and belongs to; Its Chinese name is thermophilic glucoside ground bacillus, and the Latin formal name used at school is Geobacillus thermoglucosidasius.

2. a kind of bacillus acidocldarius according to claim 1 is characterized in that this bacterial strain is a Gram-positive, and bacillus is aerobic; Produce gemma, optimum growth temperature 60-70 ℃, bacterium colony is circular on the LB substratum, and colony diameter is about 1.0-2.0 ㎜; Oyster white, smooth surface is opaque.

3. like the purposes of claim 1,2 described a kind of bacillus acidocldariuses, it is characterized in that said bacterial strain is used for the purposes that organic waste is handled.

4. like the purposes of claim 1,2 described a kind of bacillus acidocldariuses, it is characterized in that said bacterial strain is used for the purposes that glycase is produced.

5. like the purposes of claim 1,2 described a kind of bacillus acidocldariuses, it is characterized in that said bacterial strain is used for the purposes that proteolytic enzyme is produced.