CN102735852B - Determination method of giant panda estradiol true peak value - Google Patents
Determination method of giant panda estradiol true peak value Download PDFInfo
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Abstract
The invention discloses a determination method of a giant panda estradiol true peak value. According to the invention, urine samples of female giant pandas at a spring breeding period are collected and are subjected to chemical analysis; conditions such as a time for an estradiol maximal peak or a subsequent estradiol peak to decrease with an amplitude reaching a value above 50%, whether a progesterone value is in an increasing trend during the decreasing period of the estradiol maximal peak or the subsequent estradiol peak, whether the giant pandas show a typical estrous peak behavior, and a ratio of the corresponding progesterone value of the estradiol maximal peak or the subsequent estradiol peak to a progesterone basic value are combined, and the giant panda estradiol true peak value is determined. The method provided by the invention is obtained on the basis of statistical analysis of estradiol variation regularities of 78 oestrus giant pandas which constructs a maximal datum quantity currently, such that the method has high reliability. The method assists in filling up a giant panda research vacancy, and provides a scientific basis for improving captive giant panda artificial insemination conception rate.
Description
Technical field
The present invention relates to a kind ofly for the in good time test-tube basis for estimation of captive animal, particularly relate to a kind of determination methods of giant panda estradiol real peak.
Background technology
Giant panda is the distinctive Rear Endangered Wildlife of China, himself fertility is on the low side, Female Giant Panda 1 year is oestrus for once, accept natural mating and only have 1～3 day in oestrus, its ovulation period may only have one day, and Captive Giant Panda natural mating ratio is on the low side, only have at present the giant panda of 50% left and right can natural mating, there is the giant panda of half left and right to complete breeding by artificial insemination, so, determine that in good time in good time breeding of giant panda opportunity is the gordian technique that giant panda breeds.
Before the mid-90 in last century, the judgement that giant panda is bred opportunity is in good time mainly observed according to the giant panda determination of the nature of the act of oestrusing, determine that by rule of thumb giant panda breeds opportunity in good time, cause holding unclear to the in good time artificial insemination time of giant panda, lack quantizating index, this is also the major reason that causes the Giant Panda in Reproduction inefficiency.
After the mid-90 in last century, by monitoring giant panda urine in oestrus estrogen Changing Pattern, the index of utilizing the estrogen index in good time to breed as giant panda starts to be applied in giant panda artificial insemination gradually, but, to adopting the estrogen index to have very large dispute as the in good time test-tube index of giant panda, there is no unified viewpoint.
The in good time artificial insemination viewpoint of giant panda proposed at present all proposes based on estrogen peak value time of occurrence, due to giant panda heat Hormone change curve this as the undulatory property curve, there will be a plurality of peak values, and other a plurality of factors such as the giant panda ovulation also relates to progestational hormone, the behavior of oestrusing, for how definitely judging whether the estrogen peak value is that real peak there is no any relevant report.
Summary of the invention
The technical problem to be solved in the present invention is a kind ofly to carry out the determination methods that in good time artificial insemination provides the giant panda estradiol real peak of criterion for giant panda.
For solving the problems of the technologies described above, the determination methods of a kind of giant panda estradiol of the present invention real peak comprises the following steps:
(1) during breeding spring, by the time requirement, Female Giant Panda is carried out to A urine sample collection device, observe the giant panda behavior of oestrusing, on urine sample, the freezing or stored refrigerated of the laggard row of mark, then adopt enzyme linked immunosorbent assay to detect estradiol value and progesterone value to urine sample simultaneously; The described mark be recorded on urine sample mainly comprises that Female Giant Panda exhales name, urination time etc.; Described estradiol value and progesterone value refer to estradiol and the progesterone concentration value size in urine sample; Described estradiol is a kind of estrogen main in urine sample, and described progesterone is a kind of progestational hormone main in urine sample;
(2) using the giant panda urination time as horizontal ordinate, using on estradiol value and the planimetric coordinates of progesterone value as ordinate, mark respectively urination time and corresponding estradiol value and the determined two groups of coordinate points of progesterone value thereof, two groups of coordinate points are connected in turn respectively, draw out estradiol value change curve and progesterone value change curve, find out estradiol peak-peak and corresponding progesterone value thereof; Described estradiol peak-peak is also the mxm. of estradiol;
(3) calculate according to the progesterone value before estradiol peak-peak time of occurrence the Female Giant Panda progesterone basic value of oestrusing, and calculate estradiol peak-peak or progesterone value corresponding to estradiol peak value thereafter and the ratio of progesterone basic value;
(4) according to estradiol value change curve and progesterone value change curve, estradiol peak-peak or estradiol peak value are thereafter reached to the whether in rising trend and giant panda of time used more than 50%, estradiol progesterone value from the process that peak-peak or peak value are thereafter descending in fall and whether occur that the oestrus corresponding progesterone value of peak behavior, estradiol peak-peak or estradiol peak value thereafter and the situations such as ratio of progesterone basic value of typical case combines, and judges giant panda estradiol real peak jointly simultaneously; Describedly occur that the moment of giant panda estradiol real peak is corresponding giant panda urination time.
The determination methods of described a kind of giant panda estradiol real peak, if estradiol peak-peak fall in 10 hours～20 hours scopes reaches more than 50%, estradiol drops to progesterone value corresponding to basic value, estradiol peak-peak and the ratio of progesterone basic value is greater than 2 continuously afterwards, the process simultaneously descended from peak-peak at estradiol, progesterone value corresponding to estradiol peak-peak is in rising trend typical case's peak behavior of oestrusing occurs with giant panda, can determine that this estradiol peak-peak is panda estradiol real peak.
The determination methods of described a kind of giant panda estradiol real peak, if estradiol peak-peak fall in 20 hours～31 hours scopes reaches more than 50%, estradiol drops to progesterone value corresponding to basic value, estradiol peak-peak and the ratio of progesterone basic value is greater than 1.8 continuously afterwards, the process simultaneously descended from peak-peak at estradiol, progesterone value is in rising trend typical case's peak behavior of oestrusing occurs with giant panda, can determine that this estradiol peak-peak is giant panda estradiol real peak.
The determination methods of described a kind of giant panda estradiol real peak, if estradiol from peak-peak descend time of 50% more than 20 hours, progesterone value corresponding to estradiol peak-peak and the ratio of progesterone basic value is less than 1.8, after the estradiol peak-peak, occur that typical case's peak behavior of oestrusing does not appear in the not in rising trend and giant panda of progesterone value corresponding to estradiol peak-peak process that one or more peak values and estradiol descend from peak-peak, can determine that the estradiol peak-peak is false peaks simultaneously; Now, giant panda estradiol real peak is a certain peak value after the estradiol peak-peak, and this peak value must possess simultaneously, and fall in 10 hours～20 hours scopes reaches 50% or more, estradiol drops to that basic value, progesterone value that this peak value is corresponding are greater than 2 with the ratio of progesterone basic value, typical case's situations such as peak behavior of oestrusing appear in rising trend and giant panda in the estradiol progesterone value that this peak value is corresponding from the process of this peak value decline continuously afterwards.
Described 10 hours～20 hours scopes refer to that the giant panda estradiol is from the descend main criterion of 50% time used of real peak, it is to sum up in the statistics by 78 routine giant panda heat glycol Changing Patterns, the data volume of 78 examples is considerably beyond any report amount in the past, it is maximum data volume in the world up to now, 80% giant panda individuality is arranged in 78 examples all in this scope, the criterion of therefore, 10 hours～20 hours scopes has extreme high reliability.
The described giant panda typical case peak behavior of oestrusing comprises that (the special behavior of oestrusing appears to the reflection of obvious pressure tail in the peak period giant panda oestrusing, stimulate the giant panda rear quarters with bamboo pole, the quiet end reaction that stands, sticks up appears in giant panda) and behavior to be joined (quiet stand, stick up tail, trunk sinkage independently appear in giant panda).
The determination methods of described a kind of giant panda estradiol real peak, its calculating of oestrusing Female Giant Panda progesterone basic value is carried out according to the following steps:
1. choose the front all progesterone values of giant panda estradiol peak-peak, find out the maximal value A in selected progesterone value, calculate arithmetic mean value B, the standard deviation C of selected progesterone value, then draw B+2C=D;
If 2. A-D is for negative, arithmetic mean value B now is the progesterone basic value;
If 3. A-D, for just, finds out all progesterone values that are greater than D from the progesterone value of choosing, by the mode of 1. step, from residue progesterone value, find out maximal value A
1, calculate the arithmetic mean value B that remains the progesterone value
1, standard deviation C
1, then draw B
1+ 2C
1=D
1
If 4. A
1-D
1Still for just, repeat the 1. mode of step, until A
n-D
nFor negative, arithmetic mean value B now
nBe the progesterone basic value, wherein n represents natural number.
The determination methods of described any giant panda estradiol real peak, its process of Female Giant Panda being carried out to A urine sample collection device by the time requirement is as follows:
(1) observe the Female Giant Panda external genital, if after redness appears in external genital, collect 1 part of Female Giant Panda urina sanguinis every day;
(2) the 4th day from estradiol rises continuously starts, and every day, 3 parts of urine samples were collected at interval about 8 hours;
(3) before and after Female Giant Panda is oestrused the peak behavior, every day, 5 parts of urine samples were collected at interval about 5 hours;
(4) once monitor the estradiol value, downtrending is arranged, A urine sample collection device is shortened to about 3 hours interval time.
The determination methods of described any giant panda estradiol real peak, its each time the giant panda A urine sample collection device comprise the following steps:
(1) clean and dry beast and give up hardened ground;
(2) giant panda that will need to collect urine sample is put into the beast house, and the staff waits for, once giant panda is discharged urine, immediately giant panda is put into to other beast house;
(3) gather the fresh urine sample in ground by clean injectors, the freeze pipe of packing into, carry out mark, then puts into immediately freezing or cold storage plant.
Available data is without any report and correlation technique and criterion about how judging giant panda estrogen real peak, the present invention will solve the real peak that how to judge the giant panda estradiol, determine criterion, and corresponding giant panda urination time is defined as the time of occurrence of estradiol real peak by the estradiol real peak simultaneously.
The giant panda urine sample gathering can directly have influence on the judgement to peak value interval time, and interval time, real peak value was missed in long meeting, and too short one side can increase workload and, to the interference of animal, can reduce on the other hand the feasibility of sampling interval time.Uncertain and disunity of the giant panda urine sample gathering time of available data report, some acquisition times interval reaches 1 day, can cause the error in judgement scope of time to peak larger; And the sampling time interval scheme that the present invention adopts is about 3 hours in the peak period interval of oestrusing, make the error in judgement scope of giant panda estradiol time to peak was narrowed down to about 1.5 hours, greatly dwindled the error in judgement to giant panda estradiol time to peak.
Existing open report all detects merely the urine estrogen level to the judgement time of giant panda estrogen peak value, and detect oestrus peak period progesterone level and behaviouristics, does not comprehensively judge.Judge whether estrogen occurs the key of real peak is to judge whether estrogen real decline occurs, once Estrin drop appears in monitoring, actually or still can not judge the real decline of estrogen fluctuation.In order to address this problem one aspect of the present invention monitoring urine progesterone level in monitoring urine estradiol, determine simultaneously and quantize the true standard descended of estradiol, adopt the behaviouristics supplementary means comprehensively to judge whether giant panda urine estradiol is true decline.
Judge whether estradiol occurs that the key of real peak is to judge whether estradiol real decline occurs, the present invention will, by statistical study giant panda heat as much as possible glycol data, determine that the decline rate reference range of giant panda estradiol peak value quantizes to determine whether the giant panda estradiol true decline occurs.
Suspicious peak value occurs once monitor giant panda, by strengthening sampling density by continuous sampling about sampling interval time shorten to 3 hour, the speed that the monitoring estradiol descends determines whether estradiol is true decline.
Chengdu Panda Breeding Research Base has carried out statistical study to amounting to 78 routine giant panda estradiol Changing Patterns for many years, analyze estradiol from the real peak required shortest time distribution 50% or more that descends, draw out as shown in Figure 1 oestrus female giant panda estradiol from the real peak 50% time distribution frequency used that descends, adopt method of percentiles to determine the giant panda urine estradiol peak value 50% time reference value scope used that descends, the results are shown in Table 1:
Table 1. estradiol real peak 50% required time (h) that descends
By the statistics to 78 routine giant panda heat glycol Changing Patterns, find as can be seen from Table 1, giant panda urine estradiol is that 31.17 hours, minimum value are 5.83 hours from the descend maximal value of 50% required time of real peak, wherein 80% giant panda individuality is all in 9.83～19.86 hours scopes, in 10～20 hours scopes, be less than 20 hours; Analyze 16 routine giant pandas that the estradiol false peaks occurred simultaneously, analyze descend 50% required time and time thereof of estradiol peak value to distribute, the results are shown in Table 2:
Table 2. estradiol false peaks 50% required time (h) that descends
As can be seen from Table 2, for the individuality that the estradiol false peaks occurs, estradiol will be longer than real peak value individuality from false peaks 50% required time that descends, in 14.58～41.5 hours scopes, wherein 80% individuality is in 20～38.7 hours scopes, in 20～39 hours scopes, be greater than 20 hours.
Utilize these data to differentiating that judgement giant panda urine estradiol is that fluctuation or true decline provide strong quantification reference data, once monitoring giant panda urine estradiol occurs descending rapidly from a high value at short notice, if the estradiol fall reaches more than 50% in the scope of 10 hours～20 hours, can judge that this reduces to true decline; Once and occur that the estradiol decline rate is excessively slow, 50% the time of descending surpasses 20 hours, can tentatively be judged as the estradiol fluctuation, but not true decline.
In in good time test-tube open report, only adopt giant panda estrogen peak value as a reference about giant panda existing, do not monitored the urine sample progesterone level simultaneously.The present invention is when detecting giant panda urine sample estradiol, monitor giant panda urine sample progesterone level simultaneously, once the decline of estradiol occur, check that progesterone level changes simultaneously, if progesterone is without rising trend, be judged as the fluctuation of estradiol, but not true decline, if progesterone occur ascendant trend estradiol true decline may appear, by acquisition interval time of further shortening urine sample to every about 3 hours once, until the true fall time of full confirmation estradiol, thereby improved the accuracy that estradiol detects.
In order to quantize to determine giant panda corresponding progesterone level difference while true and false estradiol peak value occurring, determine the estradiol real peak in order to further quantification, compared the difference of the ratio of progesterone value that true and false estradiol peak value is corresponding and progesterone basic value, the results are shown in Table 3:
The progesterone value that the true and false estradiol peak value of table 3. is corresponding and the odds ratio of progesterone basic value are
As can be seen from Table 3,80% individuality is when the estradiol real peak occurring, and its corresponding progesterone value level raises, and the ratio of progesterone value and its basic value, between 1.79～3.14, is greater than 1.8; And, after false estradiol peak value occurring, its corresponding progesterone value level is lower, 80% individual progesterone value and the ratio of its basic value, between 0.94～2.13, are less than 2, and these data can further quantize to determine whether estradiol is real peak.After if suspicious estradiol peak value occurring, calculate the ratio of its corresponding progesterone value and progesterone basic value, can further confirm that this estradiol peak value is real peak if be greater than 2, if this ratio is less than 1.8, this estradiol peak value may be false peaks, if be greater than 1.8, need to determine further combined with the behaviouristics data.
In existing open report; less than oestrusing about the employing giant panda, whether behavior auxiliary judgment estrogen is the true relevant report descended; only Science Press in 2006, publish, the book number of the edition is p257, the author for Zhang Zhi and with " the giant panda in situ conservation theory and practice " of Wei Fuwen in related description is arranged: the time of origin of 86.4% (19/22) giant panda natural mating first is after the estrogen peak value, and the time that giant panda can carry out natural mating appears at the peak of oestrusing usually.Should " giant panda in situ conservation theory and practice " show, can assist adopt giant panda whether to occur oestrusing auxiliary reference foundation that whether the peak behavior truly descend as estrogen.
Once monitoring giant panda, the present invention occurs that estradiol descends, progesterone rises, observe the giant panda behavior of oestrusing simultaneously, if typical case's peak behavior of oestrusing appears in giant panda, but the auxiliary judgment giant panda occurs that estradiol truly descends, otherwise still can not full confirmation, this will further get rid of the situation that fluctuation appears in estradiol and progesterone simultaneously.
Because giant panda heat glycol presents the fluctuation-type change curve, once and the estradiol peak value occur, its decline rate is very fast, due to impossible continuous acquisition urine sample on giant panda, there is certain time interval in sampling, although strengthened sample frequency, still may miss real estradiol peak value sample, the estradiol peak-peak that causes the part individuality there will be to see from numerical value is the situation of real estradiol peak value not necessarily but, false peaks occurs.In existing all open reports, all less than about how rejecting the technology contents of false peaks.
Due to the present invention to current maximum amount of data 78 the example oestrus giant panda estradiol Changing Patterns carried out statistical study, shown that three kinds judge very reliably giant panda estradiol real peak and go out the method for now, wherein the urine estradiol of 80% giant panda individuality is 10 hours～20 hours from the real peak 50% required time range that descends, 80% individuality is when the estradiol real peak occurring, its corresponding progesterone value level raises, and the ratio of progesterone value and its basic value is greater than 1.8; And after the estradiol false peaks occurring, its corresponding progesterone value level is lower, the progesterone value of 80% individuality and the ratio of its basic value are less than 2, using these time ranges as main criterion, in order to quantize definite giant panda estradiol real peak and to go out now, there is extreme high reliability, filled up the blank of giant panda research field, for improving Captive Giant Panda artificial insemination pregnancy rate, provide scientific basis.
The accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 be oestrus of the present invention female giant panda estradiol from the real peak 50% time distribution frequency schematic diagram used that descends;
The first estradiol value that Fig. 2 is Female Giant Panda individuality of the present invention and the change curve of progesterone value;
The second estradiol value that Fig. 3 is Female Giant Panda individuality of the present invention and the change curve of progesterone value;
The third estradiol value that Fig. 4 is Female Giant Panda individuality of the present invention and the change curve of progesterone value.
In figure, identify: the coordinate points that α is giant panda estradiol real peak on estradiol value change curve, β is the coordinate points corresponding with α on progesterone value change curve, Ec ng/mg Cr is expressed as the estradiol concentration value that the creatinine assay method obtains, and Pg ng/mg Cr is expressed as the Concentration of Progesterone value that the creatinine assay method obtains.
Embodiment
The present invention observes Female Giant Panda vulva color every day during breed spring, the feature performance that giant panda vulva redness starts for giant panda oestruses, once find the vulva redness, can tentatively determine that giant panda enters oestrus, continue to observe every day the giant panda behavior of oestrusing, if occur, frequent marking behavior, baa can determine that behavior giant panda oestruses.
After giant panda oestruses and starts, start the Female Giant Panda urine sample is collected, recording panda on urine sample exhales name, urination time, typical case's marks such as peak behavior of oestrusing whether occur, and in time the giant panda urine sample of collecting is carried out to assay, adopt enzyme linked immunosorbent assay to detect with giant panda and exhale name and corresponding estradiol value and the progesterone value of urination time; Originally collect 1 part of urina sanguinis every day; Within the 4th day, start after the estradiol value rises continuously, collect 3 parts of urine samples every day, interval is about 8 hours, and the time is respectively 7:00,15:00,23:00 left and right; Oestrus before and after peak, every day, urine sample was increased to 5 left and right, and interval is about 5 hours, and acquisition time is respectively: 6:00,11:00,16:00,21:00,1:00 left and right; Once monitor the estradiol value, downtrending is arranged, A urine sample collection device is shortened to about 3 hours interval time, until finally confirm the true decline of estradiol value.
The giant panda A urine sample collection device is all that hardened ground from clean is collected each time, and after first clean and drying beast house ground, the giant panda that need are collected to urine sample is put into beast and gives up; Then the staff waits for, once giant panda is discharged urine, immediately giant panda is put into to other beast house, by clean injectors, gathers the fresh urine sample in ground, and the freeze pipe of packing into, carry out mark, puts into immediately the freezing preservation of freezer compartment of refrigerator or reefer stored refrigerated.
Using the giant panda urination time as horizontal ordinate, using on estradiol value and the planimetric coordinates of progesterone value as ordinate, mark respectively urination time and corresponding estradiol value and the determined two groups of coordinate points of progesterone value thereof, two groups of coordinate points are connected in turn respectively, draw out estradiol value change curve and progesterone value change curve, find out estradiol peak-peak or progesterone value corresponding to estradiol peak value thereafter, calculate the Female Giant Panda progesterone basic value of oestrusing.
The calculating of the Female Giant Panda progesterone basic value of oestrusing is carried out according to the following steps:
1. choose front progesterone value corresponding to all giant panda estradiol values of giant panda estradiol peak-peak, find out the maximal value A in selected progesterone value, calculate arithmetic mean value B, the standard deviation C of selected progesterone value, then draw B+2C=D;
If 2. A-D is for negative, arithmetic mean value B now is the progesterone basic value;
If 3. A-D, for just, finds out all progesterone values that are greater than D from the progesterone value of choosing, by the mode of 1. step, from residue progesterone value, find out maximal value A
1, calculate the arithmetic mean value B that remains the progesterone value
1, standard deviation C
1, then draw B
1+ 2C
1=D
1
If 4. A
1-D
1Still for just, repeat the 1. mode of step, until A
n-D
nFor negative, arithmetic mean value B now
nBe the progesterone basic value, wherein n is natural number.
According to estradiol value change curve and progesterone value change curve, estradiol peak-peak or estradiol peak value are thereafter reached to the whether in rising trend and giant panda of time used more than 50%, estradiol progesterone value from the process that peak-peak or peak value are thereafter descending in fall and whether occur that the oestrus corresponding progesterone value of peak behavior, estradiol peak-peak or estradiol peak value thereafter and the situations such as ratio of progesterone basic value of typical case combines, and judges giant panda estradiol real peak jointly simultaneously.The method of judgement giant panda estradiol real peak has respectively following three kinds of situations.
The first situation: as shown in Figure 2, if estradiol peak-peak fall in 10 hours～20 hours scopes reaches more than 50%, estradiol drops to progesterone value corresponding to basic value, estradiol peak-peak and the ratio of progesterone basic value is greater than 2 continuously afterwards, the process simultaneously descended from peak-peak at estradiol, progesterone value is in rising trend typical case's peak behavior of oestrusing occurs with giant panda, can determine that this estradiol peak-peak is giant panda estradiol real peak, be the α coordinate points in Fig. 2, β is the progesterone value coordinate points corresponding with α.
The second situation: as shown in Figure 3, if estradiol peak-peak fall in 20 hours～31 hours scopes reaches more than 50%, estradiol drops to progesterone value corresponding to basic value, estradiol peak-peak and the ratio of progesterone basic value is greater than 1.8 continuously afterwards, the process simultaneously descended from peak-peak at estradiol, progesterone value is in rising trend typical case's peak behavior of oestrusing occurs with giant panda, can determine that this estradiol peak-peak is giant panda estradiol real peak, be the α coordinate points in Fig. 3, β is the progesterone value coordinate points corresponding with α.
The third situation: as shown in Figure 4, if estradiol from peak-peak descend time of 50% more than 20 hours, progesterone value corresponding to estradiol peak-peak and the ratio of progesterone basic value is less than 1.8, after the estradiol peak-peak, occur process that one or more peak values and estradiol descend from peak-peak that typical case's peak behavior of oestrusing does not appear in the not in rising trend and giant panda of progesterone value, can determine that this estradiol peak-peak is false peaks simultaneously; Now, giant panda estradiol real peak is a certain peak value after the estradiol peak-peak, and this peak value must possess the in rising trend and giant panda of fall in 10 hours～20 hours scopes reaches more than 50%, estradiol drops to that progesterone value corresponding to basic value, this peak value and the ratio of progesterone basic value are greater than 2 continuously afterwards, this peak value is corresponding the process that descends from this peak value at estradiol progesterone value simultaneously and typical case's situations such as peak behavior of oestrusing occur, be the α coordinate points in Fig. 4, β is the progesterone value coordinate points corresponding with α.
Be corresponding giant panda urination time the now that goes out of described giant panda estradiol real peak.
Claims (6)
1. the determination methods of a giant panda estradiol real peak comprises the following steps:
(1) during breeding spring, by the time requirement, Female Giant Panda is carried out to A urine sample collection device, observe the giant panda behavior of oestrusing, on urine sample, the freezing or stored refrigerated of the laggard row of mark, then adopt enzyme linked immunosorbent assay to detect estradiol value and progesterone value to urine sample simultaneously;
(2) using the giant panda urination time as horizontal ordinate, using on estradiol value and the planimetric coordinates of progesterone value as ordinate, mark respectively urination time and corresponding estradiol value and the determined two groups of coordinate points of progesterone value thereof, two groups of coordinate points are connected in turn respectively, draw out estradiol value change curve and progesterone value change curve, find out estradiol peak-peak time of occurrence and corresponding progesterone value thereof;
(3) calculate according to the progesterone value before estradiol peak-peak time of occurrence the Female Giant Panda progesterone basic value of oestrusing, and calculate estradiol peak-peak or progesterone value corresponding to estradiol peak value thereafter and the ratio of progesterone basic value;
(4) according to estradiol value change curve and progesterone value change curve, estradiol peak-peak or estradiol peak value are thereafter reached to the whether in rising trend and giant panda of time used more than 50%, estradiol progesterone value from the process that peak-peak or peak value are thereafter descending in fall and whether occur that the oestrus corresponding progesterone value of peak behavior, estradiol peak-peak or estradiol peak value thereafter and the situations such as ratio of progesterone basic value of typical case combines, and judges giant panda estradiol real peak jointly simultaneously.
2. the determination methods of a kind of giant panda estradiol real peak according to claim 1, it is characterized in that: if estradiol peak-peak fall in 10 hours～20 hours scopes reaches more than 50%, estradiol drops to progesterone value corresponding to basic value, estradiol peak-peak and the ratio of progesterone basic value is greater than 2 continuously afterwards, the process simultaneously descended from peak-peak at estradiol, progesterone value is in rising trend typical case's peak behavior of oestrusing occurs with giant panda, can determine that this estradiol peak-peak is giant panda estradiol real peak.
3. the determination methods of a kind of giant panda estradiol real peak according to claim 1, it is characterized in that: if estradiol peak-peak fall in 20 hours～31 hours scopes reaches more than 50%, estradiol drops to progesterone value corresponding to basic value, estradiol peak-peak and the ratio of progesterone basic value is greater than 1.8 continuously afterwards, the process simultaneously descended from peak-peak at estradiol, progesterone value is in rising trend typical case's peak behavior of oestrusing occurs with giant panda, can determine that this estradiol peak-peak is giant panda estradiol real peak.
4. the determination methods of a kind of giant panda estradiol real peak according to claim 1, it is characterized in that: if estradiol from peak-peak descend time of 50% more than 20 hours, progesterone value corresponding to estradiol peak-peak and the ratio of progesterone basic value is less than 1.8, after the estradiol peak-peak, occur process that one or more peak values and estradiol descend from peak-peak that typical case's peak behavior of oestrusing does not appear in the not in rising trend and giant panda of progesterone value, can determine that this estradiol peak-peak is false peaks simultaneously; Now, giant panda estradiol real peak is a certain peak value after the estradiol peak-peak, and this peak value must possess simultaneously, and fall in 10 hours～20 hours scopes reaches 50% or more, estradiol drops to that basic value, progesterone value that this peak value is corresponding are greater than 2 with the ratio of progesterone basic value, typical case's situations such as peak behavior of oestrusing appear in rising trend and giant panda in the estradiol progesterone value that this peak value is corresponding from the process of this peak value decline continuously afterwards.
5. according to the determination methods of the described any giant panda estradiol real peak of claim 1～4, its process of Female Giant Panda being carried out to A urine sample collection device by the time requirement is as follows:
(1) observe the Female Giant Panda external genital, if after redness appears in external genital, collect 1 part of Female Giant Panda urina sanguinis every day;
(2) the 4th day from estradiol rises continuously starts, and every day, 3 parts of urine samples were collected at interval about 8 hours;
(3) before and after Female Giant Panda is oestrused the peak behavior, every day, 5 parts of urine samples were collected at interval about 5 hours;
(4) once monitor the estradiol value, downtrending is arranged, A urine sample collection device is shortened to about 3 hours interval time.
6. the determination methods of a kind of giant panda estradiol real peak according to claim 5, its each time the giant panda A urine sample collection device comprise the following steps:
(1) clean and dry beast and give up hardened ground;
(2) giant panda that will need to collect urine sample is put into the beast house, and the staff waits for, once giant panda is discharged urine, immediately giant panda is put into to other beast house;
(3) gather the fresh urine sample in ground by clean injectors, the freeze pipe of packing into, carry out mark, then freezing or stored refrigerated immediately.
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| CN103126728B (en) * | 2013-03-06 | 2014-03-19 | 成都大熊猫繁育研究基地 | Determination method of normal or delayed ovulation in pandas |
| CN103126783B (en) * | 2013-03-06 | 2014-05-07 | 成都大熊猫繁育研究基地 | Method for optimizing timely artificial insemination for pandas by aid of LH (luteinizing hormone) results |
| CN103207280B (en) * | 2013-03-21 | 2014-01-01 | 成都大熊猫繁育研究基地 | Method for predicting panda expected delivery date |
| CN108401916A (en) * | 2018-05-07 | 2018-08-17 | 中国大熊猫保护研究中心 | A kind of giant panda colony house |
| CN110514849A (en) * | 2019-08-24 | 2019-11-29 | 牡丹江师范学院 | A kind of super early pregnancy detection method of Northeast Female tiger |
| CN112083156B (en) * | 2020-09-18 | 2021-06-29 | 成都大熊猫繁育研究基地 | Prediction method of panda estrone peak time and application thereof |
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