CN105005934A - Simple method for comprehensively evaluating quality of boar semen - Google Patents
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Abstract
The present invention relates to the technical field of culture and particularly discloses a simple method for comprehensively evaluating the quality of boar semen. The method comprises the following steps of: firstly, detecting four indexes, namely sperm density, semen quantity, sperm viability and malformation rate; secondly, calculating the total number of active sperms according to a calculation formula; and thirdly, evaluating the quality of the boar semen according to the total number of the active sperms. According to the method, the quality of the boar semen can be objectively and accurately evaluated.
Description
Technical field
The present invention relates to cultural technique field, be specifically related to a kind of simple and easy method of Comprehensive Evaluation quality of boar semen.
Background technology
After boar semen gathers, first semen quality will be evaluated, then could be further processed seminal fluid, thus guarantee has the sperm of sufficient amount and vigor for artificial insemination, and by boar health status and breeding potential can also be monitored to semen quality evaluation, thus also can optimize individual inheritance potentiality, play maximum fertility.
The leading indicator of evaluation quality of boar semen has several aspects such as color and luster, smell, ejaculate volume, vigor density and abnormal rate.Normal semen is milky or canescence, slightly fishy smell, if be normally mixed with urine in yellow; If be normally mixed with blood in pale red; If may be have inflammation to be mixed with purulence in yellowish-brown; Color exception or frowziness person can not use, and can directly get rid of.Ejaculate volume is relevant with the ability of boar production of sperm, is generally 200 ~ 400 milliliters, and sperm sum is about 20,000,000,000 ~ 80,000,000,000, and it is stronger that semen volume represents boar production of sperm ability more, and reproductive capacity is better.Vigor refers to the ability of sperm motility, i.e. the sperm of straight ahead motion accounts for the percent of total sperm.The height of sperm motility is related to the height of breeding sow conception rate and litter size.Sperm motility evaluation is general with " ten grades of systems ", namely calculates the sperm number of linearly forward travel in a visual field.100% is 1.0 grades, and 90% is 0.9 grade, and 80% is 0.8 grade, and the rest may be inferred.Research shows: when (being less than 24h) immediately carrying out evaluation and semen deposition after semen collection, and use sperm motility lower than 62.5%, litter size/farrowing rate will decline, and lower than 0.5 grade of person, should not use.Density refers to the sperm quantity contained in every milliliter of seminal fluid, in unit area, the larger sperm quantity of density is more, the sperm concentration every milliliter of normal boar is 2.0 ~ 3.0 hundred million sperms, and 5.0 hundred million sperms up to every milliliter had, it is used to the important evidence determining semen dilution rate.Defective sperm is the sperm that paramophia has no value for use, defective sperm has the sperm such as huge sperm, dwarf spermatozoa, docking, broken end, acrosome comes off, protoplasm, head are large, double end, two tail, folding tail, generally can not rectilinear motion, fertility is poor, but does not affect the density of sperm.Rate of teratosperm refers to that defective sperm accounts for total sperm percent, and lopsided smart rate generally more than 20%, otherwise should be able to not discard.Usual seminal fluid color and luster is good, density is large, vigor is high, abnormal rate is low, drag is large, then its conception rate is high; Contrary conception rate is low.
Pass judgment on the standard of semen quality, carry out comprehensive multianalysis, except indivedual index lower than minimum value need directly superseded except, index in range of normal value, the seminal fluid of one or more mass parameters met in quality of boar semen evaluation is not able to one and draws judged result, because can not guarantee it is the seminal fluid of high-quality completely.But in actual production; if need to assess certain adjuvant or the change of certain nutritive index to when improving the test of quality of boar semen; often can find the rising that several index has; but the index had is again in reduction simultaneously; such as semen volume improves; but vigor has reduction, or deformity is improved, but density has decline.Therefore, often when many index quality disunity, those skilled in the art can be caused to be difficult to make the evaluation of seminal fluid quality, the criterion disunity of conclusion, dispute is large.
Summary of the invention
Technical matters to be solved by this invention is, in order to overcome in prior art for problems such as judge boar semen standard disunity, method are not objective, accurate, provides a kind of simple and easy method of Comprehensive Evaluation quality of boar semen.
Above-mentioned technical matters to be solved by this invention, is achieved by the following technical programs:
A kind of simple and easy method of Comprehensive Evaluation quality of boar semen, comprise following steps, first detect sperm concentration, semen volume, sperm motility and abnormal rate four indices, then calculate total effectively sperm count according to computing formula, then pass judgment on the quality of boar semen according to the size of total effectively sperm count.
The present invention is by many experiments and a large amount of summaries of experience, and having found out one only needs to detect the four indices such as sperm concentration, semen volume, sperm motility and abnormal rate, then passes judgment on the simple and easy method of quality of boar semen according to the total effectively sperm count of formulae discovery.It is objective, simple, accurate that the method is passed judgment on.Can be used for assessing certain adjuvant or the impact of certain nutritive index change on quality of boar semen.
Preferably, described computing formula is: total effectively sperm count=semen volume * density * vigor * (1-abnormal rate %).
Preferably, total effectively sperm count >=39, semen quality is qualified; Total effectively sperm count >=570, semen quality is best.
Preferably, the measuring method of described semen volume, for adopting electronic balance weighing seminal fluid, counts 1mL for every gram.
Preferably, the detection method of described sperm concentration is: measure with sperm concentration instrument.
Most preferably, the detection method of described sperm concentration is: adopt Denmark NucleoCounter SP-100 sperm concentration instrument to measure.
Preferably, the detection method of described sperm motility is: with constant temperature objective table, seminal fluid is heated to 35 ~ 37 DEG C, under 100 and 400 power microscopes, observe sperm, and namely the percent shared by sperm of straight ahead motion represents the vigor of sperm.
Preferably, the detection method of described abnormal rate is: eosin stains, measures with simple microscope.
Beneficial effect: the simple and easy method that the invention provides a kind of brand-new Comprehensive Evaluation quality of boar semen, the method only needs to detect the four indices such as sperm concentration, semen volume, sperm motility and abnormal rate, can evaluate quality of boar semen objective, accurately; In addition, the method is simple, quick, can be widely used in certain adjuvant of assessment or the impact of certain nutritive index change on quality of boar semen.
Embodiment
Explain the present invention further below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.
Embodiment 1
For the total effectively sperm count described in inspection the inventive method passes judgment on the reliability of quality of boar semen.Choose total effectively sperm count of different numerical value, sow normal and identical with 20 fecundity respectively carries out artificial insemination, detects conception rate and nest litter size.Result is as shown in table 1:
The total effectively sperm count of table 1 passes judgment on the failtests result data table of quality of boar semen
Note: numeral represents with ± standard deviation, " * " represents and checks significant difference (P<0.05) by T
Data as can be seen from table 1, total effectively sperm count is larger, and the data of conception rate and nest litter size are also larger, also just illustrates that quality of boar semen is higher.Therefore, can illustrate that method of the present invention is reliable, total effectively sperm count effectively, accurately can pass judgment on quality of boar semen.
Embodiment 2
The test situation that the organic trace element carried out at boar company water platform seed farm boar station 2-6 month in 2014 affects quality of boar semen:
Table 2. water platform seed farm organic trace element test findings
Data before and after test, after test, volume decreases 7.54ml, vigor reduces 0.01, but density improves 0.02, abnormal rate improves 0.13%, four indices test before and test after all in scope good for use, how to assess organic trace element to the impact of quality of boar semen according to this four indices, seem some difficulty, but from total effectively sperm count, effective sperm count after test decreases 11.07, can illustrate that organic trace element does not help the quality improving boar semen.
Embodiment 3
The test that the vitamin superpacket carried out at company Shi Wang in spring bis-boar stations 4-8 month in 2014 affects quality of boar semen
Table 3. stone hopes two vitamin superpacket test findings
Data before and after test, after test, boar semen volume adds 45.03ml, vigor improves 0.01, abnormal rate improves 2.51%, but density reduces 0.25, before and after test, four indices is all in scope good for use, how to assess vitamin superpacket to the impact of quality of boar semen according to this four indices, seem some difficulty, but from effective sperm count, after test, total effectively sperm count adds 26.56, shows that vitamin superpacket has certain help to the semen quality improving boar.
Embodiment 4
The different-energy hydraulic test situation that 8-12 month in 2014 carries out at the peaceful boar station, pig farm of pure and fresh branch office
Table 4. peaceful field energy hydraulic test result
Data before and after test, after test, semen volume reduces 0.757ml, density decreases 0.031, but vigor improves 0.008, abnormal rate improves 0.67%, before and after test, four indices is all in scope good for use, how to be difficult to judge the impact of energy level on boar semen kind according to these four conventional indexs, but from effective sperm count, after test, total effectively sperm count adds 3.57, show that the energy level of this test group has certain help to the semen quality improving boar, but effect is very limited.
Claims (7)
1. the simple and easy method of a Comprehensive Evaluation quality of boar semen, it is characterized in that, comprise following steps, first sperm concentration, semen volume, sperm motility and abnormal rate four indices is detected, then calculate total effectively sperm count according to computing formula, then pass judgment on the quality of boar semen according to the size of total effectively sperm count.
2. simple and easy method according to claim 1, is characterized in that, described computing formula is:
Total effectively sperm count=semen volume * density * vigor * (1-abnormal rate %).
3. simple and easy method according to claim 1, is characterized in that, total effectively sperm count >=39, and semen quality is qualified; Total effectively sperm count >=570, semen quality is best.
4. simple and easy method according to claim 1, is characterized in that, the measuring method of described semen volume, for adopting electronic balance weighing seminal fluid, counts 1 mL for every gram.
5. simple and easy method according to claim 1, is characterized in that, the detection method of described sperm concentration is: measure with sperm concentration instrument.
6. simple and easy method according to claim 1, it is characterized in that, the detection method of described sperm motility is: with constant temperature objective table, seminal fluid is heated to 35 ~ 37 DEG C, under 100 and 400 power microscopes, observe sperm, namely the percent shared by sperm of straight ahead motion represents the vigor of sperm.
7. simple and easy method according to claim 1, is characterized in that, the detection method of described abnormal rate is: eosin stains, measures with simple microscope.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107144518A (en) * | 2017-05-03 | 2017-09-08 | 安徽省农业科学院畜牧兽医研究所 | A kind of detection method of the effective sperm count of pig semen |
| CN108835031A (en) * | 2018-06-14 | 2018-11-20 | 华南农业大学 | A kind of method of the high reproductive performance herd boar of breeding |
| CN112163758A (en) * | 2020-09-25 | 2021-01-01 | 河北科技师范学院 | Method for evaluating semen grade of cock breeders of long tail chickens on dam |
| CN112586438A (en) * | 2020-11-04 | 2021-04-02 | 中国农业大学 | Method for evaluating fertility of sires |
| CN113138268A (en) * | 2020-01-19 | 2021-07-20 | 山东山大附属生殖医院有限公司 | Semen quality evaluation method, storage medium and electronic device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107144518A (en) * | 2017-05-03 | 2017-09-08 | 安徽省农业科学院畜牧兽医研究所 | A kind of detection method of the effective sperm count of pig semen |
| CN108835031A (en) * | 2018-06-14 | 2018-11-20 | 华南农业大学 | A kind of method of the high reproductive performance herd boar of breeding |
| CN113138268A (en) * | 2020-01-19 | 2021-07-20 | 山东山大附属生殖医院有限公司 | Semen quality evaluation method, storage medium and electronic device |
| CN113138268B (en) * | 2020-01-19 | 2024-03-12 | 山东山大附属生殖医院有限公司 | Semen quality evaluation method, storage medium and electronic equipment |
| CN112163758A (en) * | 2020-09-25 | 2021-01-01 | 河北科技师范学院 | Method for evaluating semen grade of cock breeders of long tail chickens on dam |
| CN112586438A (en) * | 2020-11-04 | 2021-04-02 | 中国农业大学 | Method for evaluating fertility of sires |
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Application publication date: 20151028 |