CN113138268B - Semen quality evaluation method, storage medium and electronic equipment - Google Patents
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- 210000000582 semen Anatomy 0.000 title claims abstract description 252
- 238000013441 quality evaluation Methods 0.000 title claims abstract description 57
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- 230000000694 effects Effects 0.000 abstract description 4
- 230000035558 fertility Effects 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- 230000009027 insemination Effects 0.000 description 4
- 230000035935 pregnancy Effects 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
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Abstract
The invention provides a semen quality evaluation method, a storage medium and electronic equipment, wherein the method comprises the following steps: detecting semen volume v, sperm concentration c and forward movement sperm proportion p of the semen to be evaluated; generating a semen quality evaluation model, wherein the semen volume v, the semen concentration c and the forward motile semen p are taken as independent variables, and the semen quality evaluation result is taken as a dependent variable; and obtaining a quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward motile sperms p and the evaluation model of the semen to be evaluated. According to the scheme, the semen evaluation result is obtained by not comparing each detection result with the corresponding parameter lower limit value, but integrating the detection results according to the evaluation model, so that the final judgment result of the semen quality to be evaluated is obtained, and the method has the effects of high accuracy and simple evaluation index.
Description
Technical Field
The invention relates to the technical field of semen quality research, in particular to a semen quality evaluation method, a storage medium and electronic equipment.
Background
There are many indicators for evaluating semen quality, such as liquefaction time, viscosity, semen volume, PH, sperm motility, sperm concentration, normal sperm proportion, etc. The index involved in semen quality is statistically about tens of. The detection index for determining the quality of semen is generally expressed in the form of measured values, but how to determine the quality of semen according to the peopleThe multi-index test value needs to have strong expertise to determine semen quality, and generally determines fertility according to semen index test results, for example, according to the related instructions in WHO (manual of human semen inspection and processing laboratory): the lower limit of the related numerical value of semen quality is defined as follows: the lower limit of the semen volume reference value is 1.5ml (5 th percentile, 95% confidence limit, 1.4-1.7); the lower limit of the reference value of the forward motile sperm is 32% (5 th percentile, 95% confidence interval is 31-34); the lower limit of the reference value of the sperm concentration is 15 x10 6 /ml (5 th percentile). If the detection result of any one of the above parameters does not meet the requirement of the lower limit value, it is directly determined that the semen quality is poor.
In practice, the above-mentioned evaluation criteria are very inaccurate, for example:
in the first case, if the semen volume is less than its reference lower limit, but the value of sperm concentration is high; in the second case, the semen volume and sperm concentration are both near the corresponding reference lower limits; in both cases, the number of sperms may be the same, and even the number of sperms in the first case may be more than the number of sperms in the second case, but in this case, the fertility in the first case is directly determined to be low according to the result that the semen volume is smaller than the reference lower limit value, and the fertility in the second case is qualified, which is obviously not practical.
Also for example, if the sperm cell concentration value is below its reference lower limit, but where the value of forward motile sperm cells is high, the fertility is better than in many cases (e.g., where the sperm cell concentration is equal to its reference lower limit and forward motile sperm cells is equal to its reference lower limit), and where it is not practical to determine that fertility is unacceptable only if the sperm cell concentration value is below its reference lower limit.
Based on the above, a semen quality evaluation method with high accuracy is needed.
Disclosure of Invention
The embodiment of the invention aims to provide a semen quality evaluation method, a storage medium and electronic equipment, so as to solve the technical problem of inaccurate semen quality evaluation in the prior art.
Therefore, the invention provides a semen quality evaluation method, which comprises the following steps:
detecting semen volume v, sperm concentration c and forward movement sperm p of the semen to be evaluated;
generating a semen quality evaluation model, wherein the semen volume v, the semen concentration c and the forward motile semen p are taken as independent variables, and the semen quality evaluation result is taken as a dependent variable;
and obtaining a quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward motile sperms p and the evaluation model of the semen to be evaluated.
Optionally, in the above semen quality evaluation method, an evaluation model of semen quality is generated, wherein in the step of taking the semen volume v, the semen concentration c and the forward motile sperm p as independent variables and the semen quality evaluation result as dependent variables, the evaluation model is:
the product of the sperm concentration c and the forward motile sperm p is taken as an overall value;
the semen quality evaluation results increase with the increase of the overall value, and the semen quality evaluation results increase with the increase of the forward motile sperm p.
Optionally, in the semen quality evaluation method, an evaluation model of semen quality is generated, wherein in the step of taking the semen volume v, the semen concentration c and the forward motile semen p as independent variables and taking the semen quality evaluation result as dependent variables, the evaluation model is implemented by adopting the following functions:
J＝N*ln(c*p+M)+v；
wherein J is a quality evaluation result, and N and M are adjustable parameters.
Optionally, in the semen quality evaluation method, in the evaluation model, n=10, m=1.
Optionally, in the above semen quality evaluation method, if the semen to be evaluated is in a freeze recovery state, the semen volume v therein is set to zero.
Optionally, the semen quality evaluation method further includes the following steps:
and if the J value is smaller than the set threshold value, judging that the semen to be evaluated is low in quality.
Optionally, the semen quality evaluation method further includes the following steps:
if the J value is larger than or equal to the set threshold value, and the semen volume v, the sperm concentration c and the forward motile sperm p are not lower than the corresponding minimum threshold values, judging that the semen quality to be evaluated is qualified;
and if the J value is greater than or equal to the set threshold value and any one or more of the semen volume v, the sperm concentration c and the forward motile sperm p is lower than the corresponding minimum threshold value, judging that the semen quality to be evaluated is pending.
Optionally, in the semen quality evaluation method, the set threshold is in a range of 60-65.
The invention also provides a computer readable storage medium, wherein the storage medium stores program information, and the computer executes the semen quality evaluation method after reading the program information.
The invention also provides a semen quality evaluation electronic device, which comprises at least one processor and at least one memory, wherein program information is stored in at least one memory, and the semen quality evaluation method is executed after the program information is read by at least one processor.
Compared with the prior art, the technical scheme provided by the invention has at least the following beneficial effects:
according to the semen quality evaluation method, the storage medium and the electronic equipment, after the semen volume v, the semen concentration c and the forward movement semen p of the semen to be evaluated are detected, the semen evaluation result is obtained by not comparing each detection result with the corresponding parameter lower limit value, but integrating the detection results according to the evaluation model. Compared with the evaluation mode of semen quality in the prior art, the method has the effect of high accuracy, simplifies the evaluation index, and can read the evaluation result without professional background knowledge.
Drawings
FIG. 1 is a flow chart of a semen quality evaluation method according to an embodiment of the invention;
FIG. 2 is a flowchart of a semen quality evaluation method according to another embodiment of the present invention;
fig. 3 is a schematic diagram of a hardware connection relationship of the semen quality evaluation electronic device according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of description of the present invention, and are not to indicate or imply that the apparatus or component to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between the two components. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art. In addition, the respective aspects in the following embodiments of the present invention may be combined according to actual needs as long as they do not conflict with each other.
Example 1
The embodiment provides a semen quality evaluation method, as shown in fig. 1, comprising the following steps:
and S101, detecting the semen volume v, the sperm concentration c and the forward movement sperm p of the semen to be evaluated. In the step, the semen detection equipment in the prior art can be adopted, specifically, the semen volume can be directly measured by a measuring cylinder, the sperm concentration can be obtained by adopting an artificial microscope counting mode, the Olympic microscope can be adopted, the forward movement sperm proportion can be obtained by matching with a blood cell sorter for sorting, and the blood cell sorter can be realized by adopting an Ailin blood cell sorter. The sperm concentration c is related to fertilization and pregnancy rates above; forward motile sperm p may represent a degree of forward sperm motility that correlates with pregnancy rate; the semen volume v determines the quantity of semen, and can influence the total number of forward motile sperms. Therefore, three very important indexes in semen quality analysis are selected to obtain the semen quality evaluation result, and the semen quality condition can be effectively reflected to a certain extent.
S102, generating a semen quality evaluation model, wherein the semen volume v, the semen concentration c and the forward movement sperms p are taken as independent variables, and the semen quality evaluation result is taken as a dependent variable.
S103, obtaining a quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward motile sperms p and the evaluation model of the semen to be evaluated.
In the above scheme, after the semen volume v, the semen concentration c and the forward motile semen p of the semen to be evaluated are detected, the semen evaluation result is obtained not by comparing each detection result with the corresponding parameter lower limit value, but by integrating the detection results according to the evaluation model, the final judgment result of the semen quality to be evaluated is obtained. Compared with the evaluation mode of semen quality in the prior art, the method has the effect of high accuracy, simplifies the evaluation index, and can read the evaluation result without professional background knowledge.
Preferably, in the evaluation model of the above scheme:
the product of the sperm concentration c and the forward motile sperm p is taken as an overall value; the semen quality evaluation results increase with the increase of the overall value, and the semen quality evaluation results increase with the increase of the forward motile sperm p.
The concentration of sperm c in semen and the quantity of forward motile sperm p in unit volume are jointly influenced, so that the larger the product is, the better the semen quality is, the larger the corresponding J value is, but the inventor finds that the contribution rate of c p to the J value gradually decreases along with the increase of c p in the process of realizing the invention, namely the derivative of J decreases along with the increase of c p and is always larger than 0, which basically accords with the integral trend that the derivative in a mathematical model is f' (x) =1/x, and the inventor determines a semen quality scoring formula based on the finding: the general range of J values was determined to be 0-100 in order to reasonably describe semen quality in the form of j=n (c×p+m) +y, and thus a reasonable value of N, M, Y was determined. In order to ensure that n×ln (c×p+m) > 0, the value of M should be 1. Because the number of forward motile sperm in the unit volume of the male semen has a certain range, in practical application, the value of c is about 0-20000, and the actual value of ln (c is p+1) is about 0-10, because we have determined the general range of J to be 0-100, the reasonable value of N should be 10. In addition, the semen volume v can be used as an independent factor to influence the semen quality, and in a certain range, the larger the semen volume c value is, the better the semen quality is, and the corresponding J value is also larger. Generally, the value of v is in the range of 0 to 10, so the value of Y can be calculated as the semen volume v, and the value of J is calculated to be within a certain range, and the value of J increases with the increase of the semen volume.
Thus, in summary of the above analysis, the model of semen quality score is expressed as:
j=10×ln (c×p+1) +v, ln is natural logarithm, c is sperm concentration (10) 6 Per ml), p is forward motile sperm (%), v is semen volume (ml), and the final J value is not specifically unity.
According to the model, the inventor has determined that the accuracy of the model meets the requirements and is higher than the common standard in the prior art after strict assumption, deduction and verification according to mathematical knowledge and by combining the characteristics of a plurality of semen detection indexes through analysis of mass semen sample quality data of a Shandong human sperm library.
In addition, in the above scheme, if the semen to be evaluated is in a frozen recovery state, the semen volume v therein is zero. In the process of freezing and recovering semen samples, namely, a model of semen quality freezing and recovering evaluation results is as follows: j=10×ln (c×p+1), the quality of semen after freeze recovery can be assessed by the above model. The above functions can obtain the theoretical range of J value of [0, ++ ], but the maximum calculated J value is only about 100 due to the limitation of each semen detection index, so that when the J value is more than or equal to 100, the semen quality is particularly good, and the factor for restricting the pregnancy rate is hardly caused, so that the condition that the J value is more than or equal to 100 can be statistically analyzed and processed according to the condition that the J value is=100. In summary, in practical application of the J value, the value range is [0,100], and the semen quality condition can be evaluated according to the J value fraction.
Further, as shown in fig. 2, the above method may further include the following steps:
and S104, if the J value is smaller than the set threshold value, judging that the semen to be evaluated is low in quality.
S105, if the J value is larger than or equal to the set threshold value, and the semen volume v, the sperm concentration c and the forward motile sperm p are not lower than the corresponding minimum threshold values, judging that the semen quality to be evaluated is qualified;
and S105, if the J value is larger than or equal to the set threshold value and any one or more of the semen volume v, the sperm concentration c and the forward motile sperm p is lower than the corresponding minimum threshold value, judging that the semen quality to be evaluated is pending.
Wherein the set threshold is in the interval of 60-65, preferably 60.
In the scheme, the standard of quantifying the evaluation result of the semen to be evaluated is given, and in order to better express the condition of the quality of the male semen, the preliminary evaluation of the quality of the male semen is realized by adopting a percentage score mode, so that the application in the clinical, human sperm library and scientific research fields is facilitated, and the common crowd can understand and accept the semen more easily.
The corresponding semen quality score is calculated through a J value formula, and the general condition of semen quality is simply and clearly evaluated in a score form, so that the understanding and acceptance of non-professional persons and common people on the male semen quality condition are facilitated. Meanwhile, other semen related detection indexes and clinical conditions can be combined to accurately and effectively judge male fertility. Statistical analysis of data from clinical trials and human sperm pool correlation can be compared to the J value score, which is more accurate in judging male semen quality and more acceptable to patients. Conclusion: the J value formula is reasonable in integral grading of semen quality, the obtained fraction is calculated through the semen quality grading formula, the general condition of semen quality is clearly and simply reflected in a percentage form and is easily understood by common people, the general condition can be divided into J < 60, and the semen quality is lower than the general level; j is more than or equal to 60 minutes, the semen quality is normal; j is more than or equal to 80 minutes, the semen quality before freezing reaches the human sperm pool selection standard; j'. Gtoreq.70 min, and forward motile sperm not less than 40%, the frozen resuscitated semen can be used for artificial insemination for semen supply or in vitro fertilization-embryo transfer. The J value is basically consistent with the related requirements of WHO manual of human semen inspection and treatment laboratory (5 th edition) on semen volume, semen concentration and forward motile sperms in semen quality indexes, and can be further popularized to the application in the fields of clinic, human sperm library and scientific research.
The lower limit on the semen quality-related values according to WHO manual for human semen inspection and treatment laboratory (5 th edition) is specified as follows:
the lower limit of the semen volume reference value is 1.5ml [ 5 th percentile, 95% confidence limit (CI), 1.4-1.7 ];
the lower limit of the reference value for forward motile sperm (PR) is 32% (5 th percentile, 95% confidence interval 31-34);
the lower limit of the reference value of the sperm concentration is 15 x10 6 Per ml (5 th percentile, 95% confidence interval 12-16 x 10) 6 ). Substituting the lower limit into the model can obtain:
J＝10*ln(12*31+1)+1.4＝60.6
the semen quality can just reach the normal level, which is consistent with the 60-minute set threshold standard in the scheme.
If J is less than 60, at least one value of c, p and v is lower than the lower limit of the WHO reference value, and has statistical significance, which indicates that the semen quality is lower, and corresponding measures can be taken according to clinical conditions;
if J is more than or equal to 60, but indexes lower than the lower limit of the reference value are contained in c, p and v, if the difference is not obvious, the other indexes can be understood to compensate the loss caused by the indexes to a certain extent; at this time, the semen quality can be considered to be in a qualified state;
if J is more than or equal to 60, but the condition that c, p and v take extreme values occurs, the condition of taking the extreme values must be combined, and then the semen quality is evaluated according to the clinical actual condition, and at the moment, the semen quality to be evaluated is in a pending state.
And c, p and v reach or exceed the lower limit level of the reference value, J is more than 60, and the semen quality is basically normal when the J is higher than the set threshold. Obviously, the semen quality is evaluated through the model in the scheme, wherein the demarcation point of whether the semen quality to be evaluated is qualified is J=60, and the value is consistent with the passing score of people in the prior art under the condition of scoring the percentage system. Therefore, when the semen evaluation result is seen by ordinary people, the person can easily understand that the semen evaluation result is qualified when J is more than or equal to 60, and the person does not need to further convert the semen evaluation result, so that the person without professional knowledge can understand the evaluation result more conveniently.
The inventor verifies the feasibility of the model, and the model mainly comprises the following aspect that J value can be used as an index for evaluating semen quality and applied to the clinical and human sperm library fields.
(1) Clinical application
The J value is in the form of percentage, and integrates three indexes of male semen volume, sperm concentration and forward movement sperm, wherein the indexes relate to pregnancy rate. Under the condition that strong background knowledge is not needed, the level of semen quality can be known according to the J value, and the problem of difficulty in understanding or deviation caused by cognitive level is reduced. In addition, the J value is used in combination with other semen detection indexes, and plays a positive role in male fertility assessment.
(2) Application of human sperm library
The human sperm library is used for treating sterility, preventing genetic diseases, providing reproduction insurance and the like, and the sperm is collected, detected, stored and provided by utilizing an ultralow temperature freezing technology. The J value is used as the evaluation of semen quality of human sperm library and male semen quality, and can be used for screening semen quality of donated volunteers, evaluating the quality of pooled semen samples, evaluating semen freezing recovery effect, evaluating semen quality of self-seminal preservers and the like.
(1) For volunteers donated with semen
The human sperm pool is more demanding on the quality of volunteer semen. The current human sperm pool has the semen quality requirement before freezing that the semen volume v is more than or equal to 2.0ml and the sperm concentration c is more than or equal to 50 x10 6 And (3) per ml, the forward movement sperm p is more than or equal to 50%, and the lower limit value is substituted into the model to obtain the product:
J＝10*ln(50*50+1)+2.0＝80.2；
it is shown that the pre-frozen semen quality just reaches the level of the sperm selection criteria, which is now consistent with the excellent decision threshold (80) defined in our model.
If J is more than or equal to 80, the semen quality of the volunteer in the sperm pool is judged to reach the standard required by the human sperm pool, and the standard is compared with the existing standard in the human sperm pool to obtain the results shown in Table 1:
TABLE 1 comparison of semen quality analysis and J values for Shandong human sperm pool at a certain year (mankind)
From table 1:
the sensitivity of the J value is
Specificity is as follows
The coincidence rate is as follows
The test shows that the J value provided by the invention has higher sensitivity and specificity and has excellent consistency with the current standard. Thus, J is more than or equal to 80, which indicates that the semen quality basically meets the semen quality requirement of a human sperm pool; j is less than 80, and the semen quality is not up to the requirement. I.e., j=80, can be used as a criterion as to whether the semen quality meets the semen quality requirements before freezing in the sperm pool.
(2) Semen specimen for frozen resuscitation
Because semen samples of the donated volunteers need to be divided into freezing tubes and stored in liquid nitrogen at the temperature of minus 196 ℃, the ministry of health prescribes that frozen semen for external semen supply is used for artificial insemination or in vitro fertilization-embryo transfer, the total number of forward motile sperms in each semen after freezing and recovering is not less than 40 percent, and the total number of forward motile sperms in each semen is not less than 12x10 6 。
Since the frozen resuscitated semen sample is calculated in portions and the mass and volume of the frozen resuscitated semen sample are not basically related, the semen mass after resuscitated can be evaluated by a semen mass frozen resuscitated score J' value, and the model is as follows: j' =10×ln (c×p+1).
Where ln is the natural logarithm and c is the sperm concentration (10 6 Per ml), p is forward motile sperm (%), no specific unit of formula. In general, each semen sample is 1ml, c×p is the total number of forward motile sperm in the semen sample, and when c×p is the required lower limit, n×p=1200, the above model is substituted: j' =10×ln (1200+1) =70.9.
The freezing recovery of semen just meets the required standard, and the freezing recovery is matched with a well-set threshold (70) in the scheme.
In actual work, a semen sample with J more than or equal to 80 before freezing can be subjected to a freezing recovery experiment, and if J' more than or equal to 70 and p more than or equal to 40 after freezing recovery, the semen sample can be used for insemination artificial insemination or in vitro fertilization-embryo transplantation.
(3) For self-refined preservator
The method can be combined to calculate the J value and the J' value of the semen quality, so that a preservative can better know the semen quality and the frozen resuscitated semen sample, the influence of the frozen resuscitated process on the semen quality is reflected to a certain extent, and the auxiliary reproduction is facilitated.
According to the scheme provided by the embodiment, the semen quality evaluation result can be defined according to the score dividing standard in the percentile, and the method is more easily accepted and understood by people, in other words, the semen related detection index is expressed in the form of the score, the score is high or low, the semen quality is good or bad, and the evaluation result is more concise, accurate and easy to understand, so that the general population can know the semen quality more clearly.
Example 2
The present embodiment provides a computer-readable storage medium having program information stored therein, and after reading the program information, a computer executes the semen quality evaluation method according to any one of the embodiments 1.
Example 3
The present embodiment provides an electronic device for evaluating semen quality, as shown in fig. 3, including at least one processor 301 and at least one memory 302, where at least one memory 302 stores program information, and at least one processor 301 executes the semen quality evaluation method according to any one of the embodiments 1 after reading the program information.
One processor 301 is illustrated in fig. 3. The semen quality evaluation electronics may further include: an input device 303 and an output device 304. The processor 301, memory 302, input device 303, and output device 304 may be connected by a bus or other means, for example in fig. 3.
The memory 302 serves as a non-volatile computer-readable storage medium that can be used to store non-volatile software programs, non-volatile computer-executable programs, and modules. The processor 301 executes various functional applications of the server and data processing by executing nonvolatile software programs, instructions and modules stored in the memory 302, that is, implements the semen quality evaluation method according to any one of the above-described aspects 1.
The product can execute the method provided by the embodiment of the application, and has the corresponding functional modules and beneficial effects of the execution method. Technical details not described in detail in this embodiment may be found in the methods provided in the embodiments of the present application.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (5)
1. The semen quality evaluation method is characterized by comprising the following steps of:
detecting semen volume v, sperm concentration c and forward movement sperm p of the semen to be evaluated;
generating a semen quality evaluation model, wherein the semen volume v, the semen concentration c and the forward motile semen p are taken as independent variables, and the semen quality evaluation result is taken as a dependent variable;
obtaining a quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward motile sperms p and the evaluation model of the semen to be evaluated;
in the evaluation model: the product of the sperm concentration c and the forward motile sperm p is taken as an overall value; the semen quality evaluation result increases with the increase of the overall value, and the semen quality evaluation result increases with the increase of the forward motile sperm p;
the evaluation model is realized by adopting the following functions:
J＝N*ln(c*p+M)+v；
wherein J is a quality evaluation result, n=10, m=1;
if the J value is smaller than the set threshold value, judging that the semen to be evaluated is low in quality;
if the J value is larger than or equal to the set threshold value, and the semen volume v, the sperm concentration c and the forward motile sperm p are not lower than the corresponding minimum threshold values, judging that the semen quality to be evaluated is qualified;
and if the J value is greater than or equal to the set threshold value and any one or more of the semen volume v, the sperm concentration c and the forward motile sperm p is lower than the corresponding minimum threshold value, judging that the semen quality to be evaluated is pending.
2. The semen quality evaluation method according to claim 1, wherein:
and if the semen to be evaluated is in a freezing recovery state, setting the semen volume v in the semen to be evaluated to be zero.
3. The semen quality evaluation method according to claim 2, wherein:
the set threshold is in the interval of 60-65.
4. A computer-readable storage medium, wherein program information is stored in the storage medium, and a computer executes the semen quality evaluation method according to any one of claims 1 to 3 after reading the program information.
5. Semen quality evaluation electronic device, characterized in that it comprises at least one processor and at least one memory, at least one of said memories having stored therein program information, at least one of said processors executing the semen quality evaluation method according to any of claims 1-3 after reading said program information.
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