CN113138268A - Semen quality evaluation method, storage medium and electronic device - Google Patents
Semen quality evaluation method, storage medium and electronic device Download PDFInfo
- Publication number
- CN113138268A CN113138268A CN202010061733.1A CN202010061733A CN113138268A CN 113138268 A CN113138268 A CN 113138268A CN 202010061733 A CN202010061733 A CN 202010061733A CN 113138268 A CN113138268 A CN 113138268A
- Authority
- CN
- China
- Prior art keywords
- semen
- sperm
- quality
- quality evaluation
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000582 semen Anatomy 0.000 title claims abstract description 264
- 238000013441 quality evaluation Methods 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims abstract description 47
- 230000033001 locomotion Effects 0.000 claims abstract description 28
- 238000013210 evaluation model Methods 0.000 claims abstract description 22
- 230000001419 dependent effect Effects 0.000 claims abstract description 8
- 230000015654 memory Effects 0.000 claims description 9
- 238000011084 recovery Methods 0.000 claims description 5
- 230000006870 function Effects 0.000 claims description 3
- 238000001303 quality assessment method Methods 0.000 claims 2
- 238000011156 evaluation Methods 0.000 abstract description 19
- 238000001514 detection method Methods 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 2
- 230000008014 freezing Effects 0.000 description 12
- 238000007710 freezing Methods 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 7
- 230000035558 fertility Effects 0.000 description 7
- 230000035935 pregnancy Effects 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000009027 insemination Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000019100 sperm motility Effects 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001149 cognitive effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a semen quality evaluation method, a storage medium and an electronic device, wherein the method comprises the following steps: detecting the semen volume v, the sperm concentration c and the forward movement sperm ratio p of the semen to be evaluated; generating an evaluation model of semen quality, wherein the semen volume v, the sperm concentration c and the forward movement sperm p are used as independent variables, and the semen quality evaluation result is used as a dependent variable; and obtaining the quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward movement semen p and the evaluation model of the semen to be evaluated. According to the scheme, the semen evaluation result is obtained not by comparing each detection result with the corresponding parameter lower limit value independently, but the final judgment result of the quality of the semen to be evaluated is obtained by integrating the detection results according to the evaluation model, and the method has the effects of high accuracy and simple evaluation index.
Description
Technical Field
The invention relates to the technical field of semen quality research, in particular to a semen quality evaluation method, a storage medium and electronic equipment.
Background
There are many indicators for evaluating semen quality, such as liquefaction time, viscosity, semen volume, PH, sperm motility, sperm concentration, normal sperm ratio, etc. According to statistics, the seminal fluid qualityThe related indexes are about dozens. The detection index for determining the quality of semen is generally expressed in the form of an actual measurement value, but how to determine the quality of semen according to a plurality of detection values of the index requires strong professional knowledge, and generally, fertility is judged according to the detection result of the semen index, for example, according to the relevant instructions in WHO (human semen examination and processing laboratory manual): the lower limit of the semen quality related value is defined as follows: the lower limit of the reference semen volume is 1.5ml (5 th percentile, 95% confidence limit, 1.4-1.7); the lower limit of the reference value for the forward motile sperm was 32% (5 th percentile, 95% confidence interval 31-34); the lower limit of the reference value of sperm concentration is 15 x106Ml (5 th percentile). If the detection result of any one of the above parameters does not meet the requirement of the lower limit value, the semen quality is directly judged to be poor.
In fact, the above criteria are very inaccurate, for example:
in the first case, if the volume of semen is less than its reference lower limit value, but the value of the sperm concentration is high; in the second case, the semen volume and the sperm concentration are both close to the corresponding reference lower limit values; in the above two cases, the number of sperm cells may be the same, and even the number of sperm cells in the first case may be larger than that in the second case, but it is obviously not practical to directly judge that the fertility is lower in the first case and the fertility is acceptable in the second case based on the result that the volume of semen is smaller than the reference lower limit value.
As another example, if the sperm cell concentration value is lower than the reference lower limit value, but the value of the forward-moving sperm cell is high, the fertility corresponding to this case is better than that in many cases (for example, the case where the sperm cell concentration is equal to the reference lower limit value and the forward-moving sperm cell is equal to the reference lower limit value), and it is not practical to judge that the fertility is not satisfactory only when the sperm cell concentration value is lower than the reference lower limit value.
Based on the above situation, a semen quality evaluation method with high accuracy is needed.
Disclosure of Invention
The embodiment of the invention aims to provide a semen quality evaluation method, a storage medium and electronic equipment, so as to solve the technical problem of inaccurate semen quality evaluation in the prior art.
Therefore, the invention provides a semen quality evaluation method, which comprises the following steps:
detecting the semen volume v, the sperm concentration c and the forward movement sperm p of the semen to be evaluated;
generating an evaluation model of semen quality, wherein the semen volume v, the sperm concentration c and the forward movement sperm p are used as independent variables, and the semen quality evaluation result is used as a dependent variable;
and obtaining the quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward movement semen p and the evaluation model of the semen to be evaluated.
Optionally, in the above-mentioned semen quality evaluation method, an evaluation model of semen quality is generated, wherein in the step of taking the semen volume v, the sperm concentration c and the forward motile sperm p as independent variables and taking the semen quality evaluation result as a dependent variable, in the evaluation model:
the product of the sperm concentration c and the forward moving sperm p as an overall value;
the semen quality evaluation result increases as the overall value increases, and the semen quality evaluation result increases as the forward moving sperm p increases.
Optionally, in the above method for evaluating semen quality, an evaluation model of semen quality is generated, wherein in the step of using the semen volume v, the sperm concentration c and the forward motile sperm p as independent variables and using the result of semen quality evaluation as dependent variables, the evaluation model is implemented by using the following functions:
J＝N*ln(c*p+M)+v；
wherein J is a quality evaluation result, and N and M are adjustable parameters.
Optionally, in the above semen quality evaluation method, in the evaluation model, N is 10 and M is 1.
Optionally, in the semen quality evaluation method, if the semen to be evaluated is in a frozen recovery state, the semen volume v is set to zero.
Optionally, the method for evaluating semen quality further includes the following steps:
and if the J value is smaller than the set threshold, judging that the quality of the semen to be evaluated is low.
Optionally, the method for evaluating semen quality further includes the following steps:
if the J value is greater than or equal to the set threshold value, and the semen volume v, the semen concentration c and the forward movement semen p are not lower than the corresponding minimum threshold values, judging that the semen quality to be evaluated is qualified;
and if the J value is greater than or equal to the set threshold value, and any one or more of the semen volume v, the sperm concentration c and the forward movement sperm p are lower than the corresponding lowest threshold value, judging that the semen quality to be evaluated is pending.
Optionally, in the semen quality evaluation method, the set threshold is in an interval of 60-65.
The invention also provides a computer-readable storage medium, wherein the storage medium is stored with program information, and the computer reads the program information and then executes the semen quality evaluation method.
The invention also provides an electronic device for semen quality evaluation, which comprises at least one processor and at least one memory, wherein program information is stored in the at least one memory, and the at least one processor reads the program information and then executes the semen quality evaluation method.
Compared with the prior art, the technical scheme provided by the invention at least has the following beneficial effects:
according to the semen quality evaluation method, the storage medium and the electronic equipment, after the semen volume v, the semen concentration c and the forward movement semen p of the semen to be evaluated are obtained through detection, the semen evaluation result is obtained not by comparing each detection result with the corresponding parameter lower limit value, but the final judgment result of the semen quality to be evaluated is obtained after the detection results are integrated according to the evaluation model. Compared with the evaluation mode of the semen quality in the prior art, the method has the advantages that the precision is high, the evaluation index is simplified, and the evaluation result can be read without professional background knowledge.
Drawings
FIG. 1 is a flow chart of a semen quality evaluation method according to an embodiment of the present invention;
FIG. 2 is a flow chart of a semen quality evaluation method according to another embodiment of the present invention;
fig. 3 is a schematic diagram of a hardware connection relationship of the electronic device for semen quality evaluation according to an embodiment of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description of the present invention, and do not indicate or imply that the device or assembly referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the two components can be directly connected or indirectly connected through an intermediate medium, and the two components can be communicated with each other. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. In addition, the schemes in the following embodiments of the present invention may be combined according to actual needs as long as they do not conflict with each other.
Example 1
The embodiment provides a semen quality evaluation method, as shown in fig. 1, including the following steps:
and S101, detecting the semen volume v, the sperm concentration c and the forward movement sperm p of the semen to be evaluated. In the step, the semen detection equipment in the prior art can be adopted for realization, specifically, the semen volume can be directly measured by adopting a measuring cylinder, the sperm concentration can be obtained by adopting a mode of counting by adopting an artificial microscope and can be realized by adopting an Olympus microscope, the forward movement sperm proportion can be obtained by matching with a blood cell classifier for classification, and the blood cell classifier can be realized by adopting an Ailin blood cell classifier. Above, sperm concentration c is related to fertilization and pregnancy rates; forward moving sperm p may represent the degree of forward sperm motility, which correlates with pregnancy rate; the volume of semen v determines the amount of semen that can affect the total number of forward motile sperm. Therefore, the three very important indexes in the semen quality analysis are selected to obtain the semen quality evaluation result, and the semen quality condition can be effectively reflected to a certain extent.
And S102, generating an evaluation model of the semen quality, wherein the semen volume v, the sperm concentration c and the forward movement sperm p are used as independent variables, and the semen quality evaluation result is used as a dependent variable.
S103, obtaining a quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward movement semen p and the evaluation model of the semen to be evaluated.
In the above scheme, after the semen volume v, the semen concentration c and the forward movement semen p of the semen to be evaluated are obtained through detection, the semen evaluation result is obtained not by comparing each detection result with the corresponding parameter lower limit value, but the final judgment result of the semen quality to be evaluated is obtained by integrating the detection results according to the evaluation model. Compared with the evaluation mode of the semen quality in the prior art, the method has the advantages that the precision is high, the evaluation index is simplified, and the evaluation result can be read without professional background knowledge.
Preferably, in the evaluation model of the above scheme:
the product of the sperm concentration c and the forward moving sperm p as an overall value; the semen quality evaluation result increases as the overall value increases, and the semen quality evaluation result increases as the forward moving sperm p increases.
The semen concentration c and the forward moving sperm p in the semen jointly influence the number of forward moving sperm in a unit volume, so that the product of the concentration c and the forward moving sperm p is larger, the semen quality is better, and the corresponding J value is also larger, but in the process of realizing the invention, the inventor finds that the contribution rate of c p to the J value is gradually reduced along with the increase of c p, namely the derivative of J is reduced along with the increase of c p and is always larger than 0, which basically accords with the trend that the derivative in a mathematical model is f' (x) is 1/x of the whole body, and the inventor sets the semen quality score formula as follows on the basis of the finding: a reasonable value of N, M, Y was determined by defining the general range of J values as 0-100 for a reasonable description of semen quality. In order to ensure that N × ln (c × p + M) is equal to or greater than 0, the value of M should be 1. Since the number of forwardly moving sperm per unit volume of male semen is in a certain range, in practical applications, the value of c p is about 0-20000, and the actual value of ln (c p +1) is about 0-10, since we already defined the value of J to be substantially 0-100, the reasonable value of N should be 10. In addition, the semen volume v can be used as an independent factor to influence the semen quality, and in a certain range, the larger the semen volume c value is, the better the semen quality is, and the larger the corresponding J value is. Generally, the value of v ranges from 0 to 10, so the value of Y can be taken as the semen volume v to calculate the value of J, which means that the value of J increases with the increase of the semen volume in a certain range.
Thus, in combination with the above analysis, the model for semen quality scoring is expressed as:
j10 × ln (c × p +1) + v, ln is the natural logarithm, c is the sperm concentration (10)6/ml), p is forward motile sperm (%), v is semen volume (ml), and finallyThe J value obtained has no specific units.
The inventor has confirmed that the accuracy of the model meets the requirements and is higher than the common standard in the prior art through analysis of mass semen sample quality data of a Shandong human sperm bank, combination of characteristics of a plurality of semen detection indexes according to mathematical knowledge and strict assumption, derivation and verification.
In addition, in the above scheme, if the semen to be evaluated is in a frozen resuscitation state, the semen volume v therein is zero. In the process of freezing and recovering the semen sample, namely, the model of the semen quality freezing and recovering evaluation result is as follows: j10 × ln (c × p +1), and the quality of the thawed semen can be evaluated by the above model. The theoretical range of the J value is [0, + ∞ ") as can be seen from the above function, but the calculated J value is only about 100 at the maximum due to the limitation of each semen detection index, so that when the J value is not less than 100, the semen quality is particularly good, and the J value hardly becomes a factor for restricting the pregnancy rate, and therefore, the statistical analysis can be performed on the condition that the J value is not less than 100 according to the condition that the J value is not less than 100. In conclusion, in the practical application of the J value, the value range is [0,100], and the semen quality condition can be evaluated according to the J value fraction.
Further, as shown in fig. 2, the above method may further include the following steps:
and S104, if the J value is smaller than a set threshold, judging that the quality of the semen to be evaluated is low.
S105, if the J value is larger than or equal to the set threshold value, and the semen volume v, the semen concentration c and the forward movement semen p are not lower than the corresponding lowest threshold values, judging that the quality of the semen to be evaluated is qualified;
and S105, if the J value is greater than or equal to the set threshold value, and any one or more of the semen volume v, the sperm concentration c and the forward movement sperm p is lower than the corresponding lowest threshold value, judging that the semen quality to be evaluated is undetermined.
Wherein the set threshold is in the interval of 60-65, preferably 60.
The above scheme provides a standard for quantifying the evaluation result of the semen to be evaluated, and in order to better express the quality of the male semen, the primary evaluation of the quality of the male semen is realized by adopting a percentage system mode, so that the application of the method in the fields of clinic, human sperm bank and scientific research is facilitated, and the method is easier to understand and accept by common people.
The corresponding semen quality score is calculated through a J value formula, the general situation of the semen quality is simply and clearly evaluated in a score form, and the understanding and the acceptance of non-professional persons and ordinary persons on the male semen quality situation are facilitated. Meanwhile, the fertility of the male can be accurately and effectively judged by combining other semen related detection indexes and clinical conditions. The J value is more accurate in assessing male semen quality and is more acceptable to patients, as a result of clinical testing and statistical analysis of data associated with the human sperm bank, as compared to the J value score. And (4) conclusion: the J value formula scores the semen quality reasonably, the score is calculated by the semen quality scoring formula, the general condition of the semen quality is clearly and simply reflected by a percent system form and is easily understood by common people, the general condition can be divided into J less than 60, and the semen quality is lower than the general level; if J is more than or equal to 60 minutes, the semen quality is normal; j is more than or equal to 80 minutes, the quality of the semen before freezing reaches the selection standard of the human semen bank; j' is more than or equal to 70 minutes, and the forward movement sperm is not less than 40 percent, so the frozen and recovered semen can be used for artificial insemination or in vitro fertilization-embryo transplantation. The J value is basically consistent with the requirements of WHO's Manual of human semen examination and treatment laboratory (5 th edition) on semen volume, semen concentration and forward movement semen in semen quality indexes, and can be further popularized to the application in the fields of clinic, human sperm bank and scientific research.
The lower limit of the values related to semen quality is specified in WHO manual for human semen examination and treatment laboratories (5 th edition) as follows:
the lower limit of the reference semen volume is 1.5ml [ 5 th percentile, 95% confidence limit (CI), 1.4-1.7 ];
the lower limit of the reference value of the forward motile sperm (PR) is 32% (5 th percentile, 95% confidence interval 31-34);
the lower limit of the reference value of sperm concentration is 15 x106Ml (5 th percentile)The number of bits, 95% confidence interval is 12-16 x106). Substituting the above lower limit into the model yields:
J＝10*ln(12*31+1)+1.4＝60.6
indicating that the semen quality can just reach the normal level, which is consistent with the standard of setting the threshold value for 60 points provided in the scheme.
If J is less than 60, at least one value of c, p and v is lower than the lower limit of the WHO reference value, and the value has statistical significance, which indicates that the semen quality is low, and corresponding measures can be taken according to clinical conditions;
if J is more than or equal to 60, but indexes lower than the lower limit of the reference value exist in c, p and v, if the difference is not obvious, the loss caused by the other indexes can be compensated to a certain extent; at this time, the semen quality can be considered to be in a qualified state;
if J is more than or equal to 60, but c, p and v are extreme values, the semen quality needs to be evaluated according to the clinical practical situation by combining the value taking situation, and the quality of the semen to be evaluated is in an undetermined state.
And c, p and v reach or exceed the lower limit level of the reference value, J is more than 60, and the semen quality is basically normal when the value is higher than the set threshold value. Obviously, the semen quality is evaluated through the model in the scheme, wherein the dividing point for judging whether the semen quality to be evaluated is qualified is J-60, and the value is consistent with the passing value of people for the percentage system scoring in the prior art. Therefore, for ordinary people, when seeing the semen evaluation result, the semen evaluation result can be judged to be qualified when J is greater than or equal to 60, further conversion is not needed, and the evaluation result can be more conveniently understood by people without professional knowledge.
The inventor verifies the feasibility of the model, and the method mainly comprises the following aspects that the J value can be used as an index for evaluating the semen quality and is applied to the fields of clinic and human sperm bank.
(1) Clinical application
The J value integrates three indexes of the volume of the semen, the sperm concentration and the forward movement sperm of the male in a percent mode and is related to the pregnancy rate. Under the condition of not needing strong background knowledge, the semen quality can be known at what level according to the J value, and the problem of difficult understanding or deviation caused by cognitive level is reduced. In addition, the J value is combined with other semen detection indexes to play a positive role in male fertility assessment.
(2) Application of human sperm bank
The human sperm bank is aimed at curing sterility, preventing genetic disease and providing reproductive insurance, etc. and utilizes the ultra-low temp. freezing technology to collect, detect, store and provide sperm. The human sperm bank is closely related to the quality of male semen, and the J value is used for evaluating the quality of the semen, and can be used for screening the quality of the semen of a sperm donator volunteer, evaluating the quality of a semen sample entering the bank, evaluating the semen freezing and reviving effect, evaluating the quality of the semen of a self-semen keeper and the like.
Is aimed at the volunteers who donate sperm
The requirement of the human sperm bank on the sperm quality of volunteers is higher. The requirements of the prior human sperm bank on the quality of the semen before freezing are that the semen volume v is more than or equal to 2.0ml, and the semen concentration c is more than or equal to 50 x106And/ml, the forward movement sperm p is more than or equal to 50 percent, and the lower limit value is substituted into the model to obtain:
J＝10*ln(50*50+1)+2.0＝80.2；
indicating that the semen quality just reached the level of sperm inclusion criteria before freezing, at this time, coinciding with the excellent decision threshold (80) defined in our model.
If J is more than or equal to 80 and is used as a standard for evaluating whether the semen quality meets the requirements of the human sperm bank, whether the semen of the volunteer in the sperm bank meets the standard or not is divided, the standard is compared with the current standard of the human sperm bank, and the obtained result is shown in the table 1:
TABLE 1 comparison of sperm quality analysis and J-value (human grade) of the human sperm bank in Shandong at a certain age
From table 1, it can be seen:
sensitivity of J value of
With a specificity of
The coincidence rate is
Tests show that the J value provided by the invention has higher sensitivity and specificity and excellent consistency with the current standard. Therefore, J is more than or equal to 80, which indicates that the semen quality of the semen meets the requirement of the semen quality of the human semen bank basically; j is less than 80, the semen quality is not up to the requirement. Namely J-80 can be used as the standard whether the semen quality meets the semen quality requirement before freezing in the semen bank.
② aiming at frozen and resuscitated semen sample
Because the semen specimen of the sperm donor volunteer needs to be separately packaged in a freezing tube and stored in liquid nitrogen at-196 ℃, the hygiene department stipulates that the frozen semen for the artificial insemination of the semen or the in vitro fertilization-embryo transplantation is applied to the external semen supply, the number of the forward movement sperms is not less than 40 percent after the frozen resuscitation, and the total number of the forward movement sperms in each semen is not less than 12x106。
Because the semen sample of frozen resuscitation is calculated according to the portion, the quality and the volume of the semen sample are basically irrelevant, so the semen quality after the evaluation of resuscitation can be expressed by the value of the semen quality frozen resuscitation score J', and the model is as follows: j' ═ 10 × ln (c × p + 1).
Wherein ln is the natural logarithm, and c is the sperm concentration (10)6And/ml), p is forward movement sperm (%), and the formula has no specific unit. Generally, each semen sample is 1ml, where c × p is the total number of forward-motile sperm in the semen sample, and when c × p is equal to the required lower limit, n × p is 1200, and substituted into the sampleThe model is as follows: j' ═ 10 × ln (1200+1) ═ 70.9.
It is stated that the freeze-recovery of semen just meets the required criteria, which at this point coincides with the well-established threshold (70) in the protocol.
In practical work, the semen sample with J being more than or equal to 80 before freezing can be subjected to freezing recovery experiment, and if J' is more than or equal to 70 after freezing recovery and p is more than or equal to 40, the semen sample can be used for artificial insemination or in-vitro fertilization-embryo transplantation.
③ for preserving sperm
The method can be combined to calculate the J value and the J' value of the semen quality, so that a keeper can better know the semen quality of the keeper and the frozen and recovered semen sample, the influence of the frozen and recovered process on the semen quality is reflected to a certain degree, and the assisted reproduction is convenient to carry out.
According to the scheme provided by the embodiment, the evaluation result of the semen quality can be defined according to the dividing standard of the scores in the percentage system, the form is more easily accepted and understood by people, in other words, the semen related detection indexes are expressed in the form of the scores, the degree of the scores represents the quality of the semen, the evaluation result is more concise, accurate and easy to understand, and the general population can more clearly know the semen quality.
Example 2
The embodiment provides a computer-readable storage medium, wherein program information is stored in the storage medium, and after the program information is read by a computer, the method for evaluating the semen quality according to any one of the embodiments 1 is executed.
Example 3
The present embodiment provides an electronic device for semen quality evaluation, as shown in fig. 3, comprising at least one processor 301 and at least one memory 302, wherein program information is stored in at least one of the memories 302, and after the program information is read by at least one of the processors 301, the semen quality evaluation method according to any of the embodiments 1 is executed.
In fig. 3, one processor 301 is taken as an example. The semen quality evaluation electronic device may further include: an input device 303 and an output device 304. The processor 301, the memory 302, the input device 303 and the output device 304 may be connected by a bus or other means, and fig. 3 illustrates the connection by a bus as an example.
The product can execute the method provided by the embodiment of the application, and has the corresponding functional modules and beneficial effects of the execution method. For technical details that are not described in detail in this embodiment, reference may be made to the methods provided in the embodiments of the present application.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A semen quality evaluation method is characterized by comprising the following steps:
detecting the semen volume v, the sperm concentration c and the forward movement sperm p of the semen to be evaluated;
generating an evaluation model of semen quality, wherein the semen volume v, the sperm concentration c and the forward movement sperm p are used as independent variables, and the semen quality evaluation result is used as a dependent variable;
and obtaining the quality evaluation result of the semen to be evaluated according to the semen volume v, the semen concentration c, the forward movement semen p and the evaluation model of the semen to be evaluated.
2. The semen quality evaluation method according to claim 1, wherein an evaluation model of semen quality is generated in which in the step of taking the semen volume v, the sperm concentration c, and the forward-motile sperm p as independent variables and the semen quality evaluation result as dependent variables, in the evaluation model:
the product of the sperm concentration c and the forward moving sperm p as an overall value;
the semen quality evaluation result increases as the overall value increases, and the semen quality evaluation result increases as the forward moving sperm p increases.
3. The semen quality evaluation method according to claim 2, wherein an evaluation model of the semen quality is generated, wherein in the step of taking the semen volume v, the sperm concentration c and the forward motile sperm p as independent variables and the semen quality evaluation result as a dependent variable, the evaluation model is implemented by using the following functions:
J＝N*ln(c*p+M)+v；
wherein J is a quality evaluation result, and N and M are adjustable parameters.
4. The semen quality evaluation method according to claim 3, characterized in that:
in the evaluation model, N is 10 and M is 1.
5. The semen quality evaluation method according to claim 4, characterized in that:
and if the semen to be evaluated is in a frozen recovery state, setting the semen volume v to be zero.
6. The semen quality evaluation method according to any one of claims 1 to 5, further comprising the steps of:
and if the J value is smaller than the set threshold, judging that the quality of the semen to be evaluated is low.
7. The semen quality evaluation method according to claim 6, further comprising the steps of:
if the J value is greater than or equal to the set threshold value, and the semen volume v, the semen concentration c and the forward movement semen p are not lower than the corresponding minimum threshold values, judging that the semen quality to be evaluated is qualified;
and if the J value is greater than or equal to the set threshold value, and any one or more of the semen volume v, the sperm concentration c and the forward movement sperm p are lower than the corresponding lowest threshold value, judging that the semen quality to be evaluated is pending.
8. The semen quality evaluation method according to claim 7, characterized in that:
the set threshold is in the interval of 60-65.
9. A computer-readable storage medium, wherein program information is stored in the storage medium, and a computer reads the program information and executes the semen quality evaluation method according to any one of claims 1 to 8.
10. An electronic device for semen quality assessment, comprising at least one processor and at least one memory, wherein at least one memory stores program information, and at least one processor reads the program information and executes the semen quality assessment method according to any one of claims 1 to 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010061733.1A CN113138268B (en) | 2020-01-19 | 2020-01-19 | Semen quality evaluation method, storage medium and electronic equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010061733.1A CN113138268B (en) | 2020-01-19 | 2020-01-19 | Semen quality evaluation method, storage medium and electronic equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113138268A true CN113138268A (en) | 2021-07-20 |
| CN113138268B CN113138268B (en) | 2024-03-12 |
Family
ID=76808961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010061733.1A Active CN113138268B (en) | 2020-01-19 | 2020-01-19 | Semen quality evaluation method, storage medium and electronic equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113138268B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10185919A (en) * | 1996-12-27 | 1998-07-14 | Fuso Yakuhin Kogyo Kk | Evaluation method for man's sperm fertility |
| CN105005934A (en) * | 2015-07-06 | 2015-10-28 | 广东温氏食品集团股份有限公司 | Simple method for comprehensively evaluating quality of boar semen |
| CN107144518A (en) * | 2017-05-03 | 2017-09-08 | 安徽省农业科学院畜牧兽医研究所 | A kind of detection method of the effective sperm count of pig semen |
| RU2700454C1 (en) * | 2018-11-07 | 2019-09-17 | Федеральное государственное бюджетное учреждение высшего образования "Южно-Уральский государственный аграрный университет" | Method for prediction of boar male sperm quality |
-
2020
- 2020-01-19 CN CN202010061733.1A patent/CN113138268B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10185919A (en) * | 1996-12-27 | 1998-07-14 | Fuso Yakuhin Kogyo Kk | Evaluation method for man's sperm fertility |
| CN105005934A (en) * | 2015-07-06 | 2015-10-28 | 广东温氏食品集团股份有限公司 | Simple method for comprehensively evaluating quality of boar semen |
| CN107144518A (en) * | 2017-05-03 | 2017-09-08 | 安徽省农业科学院畜牧兽医研究所 | A kind of detection method of the effective sperm count of pig semen |
| RU2700454C1 (en) * | 2018-11-07 | 2019-09-17 | Федеральное государственное бюджетное учреждение высшего образования "Южно-Уральский государственный аграрный университет" | Method for prediction of boar male sperm quality |
Non-Patent Citations (5)
| Title |
|---|
| WU JUNQING ET AL.,: "Reference value of semen quality in Chinese young men", CONTRACEPTION, 3 June 2002 (2002-06-03), pages 365 - 369 * |
| 余玲玲;陈小剑;江明华;丁琴丽;杨海蔚;: "精子动态参数在精液质量分析中的应用", 医学研究杂志, no. 06, 15 June 2008 (2008-06-15) * |
| 武俊青, 高尔生, 杨秋英, 梁翠玲, 陶建国, 薄立伟, 李玉仙, 周玉梅, 姚康寿, 陆卫群, 陈路, 杜银梅: "中国年轻男性精液质量主成分分析", 中国卫生统计, no. 04, pages 217 * |
| 王奇玲;: "因子分析方法在精液质量综合评价中的应用", 中华男科学杂志, no. 08, 20 August 2012 (2012-08-20) * |
| 高尔生, 武俊青, 杨秋英, 李文英, 徐晓, 付占海, 郭健, 蔡文娟, 唐锋, 陆卫群: "562例正常男子精液质量的综合评价", 中国公共卫生, no. 06, pages 674 - 675 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113138268B (en) | 2024-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Björndahl et al. | The sixth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen: ensuring quality and standardization in basic examination of human ejaculates | |
| Sharma et al. | TUNEL as a test for sperm DNA damage in the evaluation of male infertility | |
| Lu et al. | Computer‐aided sperm analysis: past, present and future | |
| Mclaughlin et al. | Kidney biopsy in systemic lupus erythematosus | |
| Schaff et al. | Novel centrifugal technology for measuring sperm concentration in the home | |
| Hinting et al. | Capacity of objectively assessed sperm motility characteristics in differentiating between semen of fertile and subfertile men | |
| CN115482925A (en) | Infection detection and differentiation system and method | |
| Tomlinson et al. | CASA in the medical laboratory: CASA in diagnostic andrology and assisted conception | |
| Douglas et al. | A novel approach to improving the reliability of manual semen analysis: a paradigm shift in the workup of infertile men | |
| Comhaire et al. | OPINION: Objective semen analysis: has the target been reached? | |
| WO2022100040A1 (en) | Method and device for detecting specific protein reaction | |
| Lammers et al. | Comparison of two automated sperm analyzers using 2 different detection methods versus manual semen assessment | |
| Dias et al. | Sperm assessment: Traditional approaches and their indicative value | |
| CN112837818B (en) | Model for evaluating liver fibrosis degree of hepatitis B patient | |
| Mortimer et al. | Workshop report: clinical CASA--the quest for consensus | |
| Centola | Comparison of manual microscopic and computer-assisted methods for analysis of sperm count and motility | |
| CN113138268A (en) | Semen quality evaluation method, storage medium and electronic device | |
| Mortimer | Laboratory standards in routine clinical andrology | |
| RU2587333C1 (en) | Method for prediction of pregnancy in vitro fertilisation and embryo transfer | |
| CN110554204B (en) | Method for diagnosing/early warning perinatal fatty liver dairy cows by using 3 serum biochemical indexes | |
| Sokol et al. | Comparison of two methods for the measurement of sperm concentration | |
| Hall et al. | A pilot study to determine the feasibility of collecting amniotic fluid samples from women during labour and measuring amniotic fluid lactate at point of care | |
| RU2582979C1 (en) | Method of updating stage of advanced cervical cancer | |
| Macleod et al. | Assessment of the conventional criteria of semen quality by computer-assisted image analysis: evaluation of the Hamilton–Thorn motility analyser in the context of a service andrology laboratory | |
| Maree | Standard Semen Analysis: Computer-Assisted Semen Analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |