Background technology
Semen quality is an important indicator of boar reproductive capacity, and semen quality is bad to be one of sterile major reason of boar.Produce for Pig Industry, quality of boar semen is bad to be a kind of problem of frequent appearance, and the bad boar of semen quality is when this is handed over, and often can not normally become pregnant because of sow causes returning feelings behind the insemination of sows; And when artificial insemination, semen quality is bad to be caused seminal fluid not use and discards.
During insemination of sows, enter the common total effectively sperm count that determines to enter genital tract of total semen volume, sperm concentration, sperm motility of genital tract.Therefore ejaculate volume is not enough, is largely determining total effectively deficiency of sperm count.Generally, the ejaculate volume of boar is not enough, and many because the production of sperm ability decline of boar causes, and therefore other index with seminal fluid has sizable correlation, the size of semen volume has also to a certain degree represented the height of the sexual function of boar.
Testis is kept the temperature of normal production of sperm ability than low 3~5 ℃ of its body temperature, if environment temperature is higher than 28 ℃, then the adjustment of the testicular vessel clump of boar and scrotum can't keep the normal temperature range of testis, thereby cause production of sperm power to descend, ejaculate volume also can reduce, and also can have influence on the indexs such as sperm concentration, vigor, sperm morphology simultaneously.Therefore, under summer high temperature environment, improve boar immunity, strengthen boar to the stress ability of summer high temperature environment, very important to the normal production of sperm ability of keeping testis.
" sow is good, and is good a brood of; Boar is good, a slope ", this has proved absolutely the importance of herd boar.Often there is following problem in each intensive pig farm herd boar but at present: semen quality descends; Sexual hypoesthesia; Leg is sick, hoof disease is multiple; Mortality is high.These problems cause very large economic loss for intensive pig farm, and for this reason, the raiser uses antibiotic to carry out medicinal health-care in a large number, although receive certain effect, medicament residue and the pathogenic microorganism resistance to the action of a drug strengthen gradually, cause the pig immunosupress, have endless trouble.
Summary of the invention
The invention provides boar plant feed addictive, premix and a batch.
Plant feed addictive provided by the present invention, its purpose is to strengthen herd boar physique, improves quality of boar semen, improves the reproductive capacity of herd boar.
At first, the invention provides a boar plant feed addictive, it contains the component of following weight parts:
The fruit of Chinese magnoliavine 20 ~ 40;
Pine needle 20 ~ 40;
Gorgon fruit 5 ~ 15;
Radix Glycyrrhizae 5 ~ 15;
The vine of multiflower knotweed 3 ~ 10.
Further, feed addictive of the present invention contains the component of following weight parts:
The fruit of Chinese magnoliavine 25;
Pine needle 30;
Gorgon fruit 10;
Radix Glycyrrhizae 10;
The vine of multiflower knotweed 5.
Wherein, described pig is preferably herd boar.
In a specific embodiments of the present invention, feed addictive of the present invention also can contain calcium carbonate and/or cornstarch as carrier.
The present invention also provides the preparation method of described feed addictive, and it is with the component grind into powder of formula rate, mixes rear sterilization.
The present invention also provides described feed addictive strengthening herd boar physique, improves quality of boar semen, improves the application in the herd boar reproductive capacity.
Particularly, add in the basal diet of herd boar with the addition of 500 ~ 1000mg/Kg, preferred, add in the basal diet of herd boar with the addition of 750mg/Kg.
Further, feed addictive of the present invention can add the premix of herd boar to, in corn bean pulp type premix, therefore the invention provides the premix that contains described feed addictive.
Further, feed addictive of the present invention can directly add in the batch of herd boar, maybe the premix that contains described feed addictive is added in the batch of herd boar, and therefore, the present invention also provides the batch that contains described feed addictive.
The present invention is theoretical and compatibility of drugs theory according to Chinese herbology, takes full advantage of the synergy of various Chinese herbal medicines, and reasonable compatibility is brought into play its comprehensive effect, develops compound Chinese herb feed additive.The fruit of Chinese magnoliavine contains abundant organic acid, vitamin, flavonoids, phytosterol and the lignan of potent reactivation is arranged, it also is one of minority medicinal material that has concurrently essence, gas, refreshing three large helps, the strong liver of the beneficial gas of energy, the efficient of promoting the cells exclude refuse, supply more oxygen, construction and uses energy, raising endurance.Pine needle is rich in protein, antibiotic, chlorophyll, string, plant enzyme, 8 seed amino acids and lot of trace ore deposit element, multivitamin isoreactivity material.Theoretical according to drug matching, the fruit of Chinese magnoliavine cooperates the nutritional labelings such as pine needle, Gorgon fruit, Radix Glycyrrhizae, the vine of multiflower knotweed, can obviously reduce the generation of harmful free radical, peroxide.Therefore plant additive of the present invention can strengthen herd boar physique to a certain extent, improves quality of boar semen, improves the reproductive capacity of herd boar.Licorice powder in the plant additive of the present invention also has special fragrance, and herd boar is had the food calling effect.Product of the present invention also have price low, produce and use the characteristics such as simple, that addition is few, additive effect is obvious, have no adverse reaction.
The specific embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Prepare plant feed addictive 100kg of the present invention, formed by fruit of Chinese magnoliavine 25kg, pine needle 30kg, Gorgon fruit 10kg, Radix Glycyrrhizae 10kg, vine of multiflower knotweed 5kg and cornstarch 20kg respectively.With described component grind into powder, mix rear sterilization, namely get plant feed addictive of the present invention.
Wherein, the fruit of Chinese magnoliavine originates in the north, Heilongjiang Province available from medicinal material wholesale market, Harbin, is wild fructus schisandrae dry mature fruit, crosses 40 mesh sieves after pulverizing.All the other medicinal materials all can be in the commercially available acquisition of pharmacy.
Experimental animal and experimental design
Select Xiping, Henan to test clinical health, 250 kilograms ~ 300 kilograms of body weight according to 12 of the large white race boars on Feng Muye Co., Ltd original seed pig farm.1 group is the blank group, the basal diet of feeding; 2,3 and 4 groups is test group.2 groups of plant feed addictives that add the preparation of 500mg/Kg present embodiment are low dose group; 3 groups of plant feed addictives that add the preparation of 750mg/Kg present embodiment are middle dosage group; 4 groups of plant feed addictives that add the preparation of 1000mg/Kg present embodiment are high dose group.The plant feed addictive of research variable concentrations is mainly measured the indexs such as semen volume, former precision, sperm motility rate to the impact of quality of boar semen.
Experimental basis Diet Nutrient content all meets or exceeds NRC(1994) standard, diet forms and trophic level sees Table 1.
The composition of the basic diet of table 1 and trophic level
1)Premix provides for every kilogram of diet: VA 7000IU, VD32500IU, VE 36mg, VK32mg, VB120.025mg, VB25.6mg, VB64mg, nicotinic acid 38mg, folic acid 1.1mg, calcium pantothenate 10mg, biotin 0.16mg, Cu 10mg, Fe 70mg, Mn 100mg, Zn 70mg, I 0.55mg, Se 0.12mg.
2)Except metabolizable energy, each value of all the other trophic level is measured value.
Undertaken by the pig farm Routine Management.Carry out simultaneously immunization campaign, expelling parasite.Enter and just try after date, each component corresponding daily ration of feeding.Free choice feeding is freely drunk water, and carries out immunity by the pig farm normal procedure.Testing program sees Table 2.
Table 2 experimental design scheme
2. the collection of seminal fluid
Seminal fluid adopts the artificial vagina collection, and seminal fluid was transported to the laboratory in 37 ℃ of preservations after gathering within 30 minutes, then with 4 layers of clean filtered through gauze to being preheating in 37 ℃ the container, with the body material that removes photoresist.After diluted, at room temperature place 1 hour, and then be stored in 17 ℃ the water-bath.
3. detect index and method
Semen quality checks
Semen volume checks: directly with the smart cup of collection scale reading.
Former precision inspection: with physiological saline seminal fluid was diluted according to 1: 5, then on the sperm concentration analyzer, directly measure.
Sperm motility checks: under 18 ℃~25 ℃ of room temperatures, get a seminal fluid and add cover glass on slide, put test under microscope sperm motility situation, and the estimation forward motile sperm accounts for the percentage of sperm in the whole visual field.
Sperm motility rate checks: adopt the blue intravital stain of Yihong-aniline and twice counting method inspection of hemacytometer.Get first a seminal fluid sample drop on slide, add an Eosin Y dye liquor mixing 5 minutes, add 2 of the blue dye liquors of aniline and mix smear, observe under 1000 power microscopes after natural drying, the sperm of living is white in color because dyestuff can not enter, and Necrospermia is dyed to pink colour; Twice counting method of hemacytometer remembers that for the first time Necrospermia counts A1, and the note sperm count A2 that lives adds up 500 sperms, at least according to formula A(% in every sample for the second time)=the sperm percentage of living A1/(A1+A2) * 100 calculated.For avoiding damaging sperm, the same gentleness of dye liquor and seminal fluid etc. is oozed, and the pH value is 6.8.
4. results and analysis
As can be seen from Table 3, compare with control group, test 2 groups and 4 groups ejaculate volume, density and vigor significant difference of test, testing 3 groups ejaculate volume, density and vigor, compare difference with control group extremely remarkable, and test 2 groups and test that to compare smart amount, density and vigor difference between 4 groups not remarkable.
The horizontal herd boar semen quality of the different interpolations of table 3 plant additive
Group |
Ejaculate volume cm
3 |
Density/hundred million mL |
Vigor % |
1 group |
165.83±71.62a |
2.25±0.75a |
74.89±5.32a |
2 groups |
172.36±54.25a |
2.53±1.12a |
80.36±2.36a |
3 groups |
236.08±70.45aa |
2.78±0.36aa |
82.65±5.65aa |
4 groups |
189.36±65.35a |
2.65±0.86a |
81.74±6.32a |
Annotate: with the column data shoulder motes
aRepresentative is compared significant difference with control group,
AaRepresentative differs extremely remarkable with control group;
Embodiment 2
Prepare plant feed addictive 100kg of the present invention, formed by fruit of Chinese magnoliavine 20kg, pine needle 40kg, Gorgon fruit 8kg, Radix Glycyrrhizae 10kg, vine of multiflower knotweed 8kg and maize cob meal 14kg respectively.With described component grind into powder, mix rear sterilization, namely get plant feed addictive of the present invention.
The feed addictive that the makes addition with 750mg/Kg is added in the basal diet of herd boar, and the plant feed addictive of research present embodiment preparation is to the research of the aspects such as the smart amount of herd boar, density and vigor, and method is with embodiment 1.
Not add the negative control group of feed feed addictive, as shown in Table 4, the result shows that the smart amount of vegetalitas additive group herd boar, density compares significant difference with vigor.
The horizontal herd boar semen quality of the different interpolations of table 4 plant additive
Group |
Ejaculate volume cm
3 |
Density/hundred million mL |
Vigor % |
Control group |
156.65±75.42
a |
2.18±0.65
a |
73.49±5.28
a |
Test group |
162.16±55.45
a |
2.43±0.82
a |
79.36±1.36
a |
Annotate: with the column data shoulder motes
aRepresentative is compared significant difference with control group,
AaRepresentative differs extremely remarkable with control group
Embodiment 3
Prepare plant feed addictive 100kg of the present invention, formed by fruit of Chinese magnoliavine 30kg, pine needle 35kg, Gorgon fruit 10kg, Radix Glycyrrhizae 10kg, vine of multiflower knotweed 8kg and maize cob meal 7kg respectively.With described component grind into powder, mix rear sterilization, namely get plant feed addictive of the present invention.
The feed addictive that the makes addition with 750mg/Kg is added in the basal diet of herd boar, and the plant feed addictive of research present embodiment preparation is to the research of the aspects such as the smart amount of herd boar, density and vigor, and method is with embodiment 1.
Not add the negative control group of feed feed addictive, as shown in Table 5, the result shows that the smart amount of vegetalitas additive group herd boar, density compares significant difference with vigor.
The horizontal herd boar semen quality of the different interpolations of table 5 plant additive
Group |
Ejaculate volume cm
3 |
Density/hundred million mL |
Vigor % |
Control group |
159.56±74.32
a |
2.08±0.63
a |
72.86±5.18
a |
Test group |
161.99±54.45
a |
2.33±0.62
a |
78.36±1.38
a |
Annotate: with the column data shoulder motes
aRepresentative is compared significant difference with control group,
AaRepresentative differs extremely remarkable with control group
Embodiment 4
Prepare plant feed addictive 100kg of the present invention, formed by fruit of Chinese magnoliavine 40kg, pine needle 40kg, Gorgon fruit 5kg, Radix Glycyrrhizae 5kg, vine of multiflower knotweed 3kg and calcium carbonate 7kg respectively.With described component grind into powder, mix rear sterilization, namely get plant feed addictive of the present invention.
The feed addictive that the makes addition with 750mg/Kg is added in the basal diet of herd boar, and the plant feed addictive of research present embodiment preparation is to the research of the aspects such as the smart amount of herd boar, density and vigor, and method is with embodiment 1.
Not add the negative control group of feed feed addictive, as shown in Table 6, the result shows that the smart amount of vegetalitas additive group herd boar, density compares significant difference with vigor.
The horizontal herd boar semen quality of the different interpolations of table 6 plant additive
Group |
Ejaculate volume cm
3 |
Density/hundred million mL |
Vigor % |
Control group |
158.34±72.15
a |
2.63±0.89
a |
71.78±5.06
a |
Test group |
163.49±53.46
a |
2.73±082
a |
79.36±1.58
a |
Annotate: with the column data shoulder motes
aRepresentative is compared significant difference with control group,
AaRepresentative differs extremely remarkable with control group
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.