CN110514849A - A kind of super early pregnancy detection method of Northeast Female tiger - Google Patents
A kind of super early pregnancy detection method of Northeast Female tiger Download PDFInfo
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Abstract
The present invention relates to a kind of super early pregnancy detection methods of Northeast Female tiger, step 1: the freshly voided urine of continuous acquisition tigress mating season；Step 2: the continuous acquisition tigress mate after the 1st week freshly voided urine；Step 3: the collected whole urine preparings of above two step are measured the progesterone level in urine sample；Step 4: the 1st week urine progesterone level surveyed after mating season and mating is compared and analyzed using SPSS statistical software；If the 1st week urine progesterone average level is significantly higher than the urine progesterone average level of mating season after tigress mating, so that it may judge this Northeast Female tiger successful pregnancy.This method can make Captive Population be more convenient for managing, it no longer needs that tiger is allowed to carry out secondary mating to avoid fighting, no longer need to pay close attention to tigress heat again, and better eating condition can be provided for the tigress of gestation, no longer need to be judged according to formalness tigress whether successful pregnancy, judge that effect is accurate.
Description
Technical field
The present invention relates to the methods for judging animal pregnancy, and in particular to a kind of super early pregnancy detection method of Northeast Female tiger.
Background technique
It is well known that artificial feeding is a kind of effective measures of China's northeastern tiger in situ conservation or ex situ conservation, to protection
The gene diversity and population scale of northeastern tiger are all a kind of fabulous measures.Although current China's artificial feeding Captive Population
There is preferable quantity size, but the quantity of wild northeastern tiger is still few, is already at Critical Condition；And it will manually raise
It is to expand one of the method for wild stocks that feeding northeastern tiger, which carries out open countryization,.However open countryization needs sufficiently large Artificial Population and foot
As guarantee, the Captive Population quality and quantity of the current artificial feeding of this point is not still able to satisfy to be needed the individual of enough health
It asks.For the population quantity and quality of further expansion northeastern tiger artificial feeding, it is also necessary to further strengthen artificial feeding northeast
The breeding scale of tiger.
Tiger post-coitum, administrative staff the most concern is tigress whether successful pregnancy, and it is more early judgement more convenient for management.
This is mainly reflected in the following aspects:
If 1, can determine the pregnancy status of tigress ahead of time, there is no need to allow male and female tiger to carry out secondary mating again, which reduce female
Recurrent phenomenon of fighting in male tiger mating gives these precious " provenance tiger " bring injuries to reduce；
2, raiser is for the needs of breeding, moment concern tigress whether heat, it is thus identified that the gestation of tigress, there is no need to again
Moment pays close attention to whether she heat phenomenon occurs again, so as to mitigate the work load of raiser；
3, once it is determined that gestation, raiser can reduce chicken and duck meat and supply and increase to provide better board and lodging condition for tigress
The supply for adding beef provides environment better colony house.
However, the gestation judgement of northeastern tiger is judged according to formalness observation at present, lack accuracy.Especially
The judgement for early pregnancy because the tigress gestation front and back bodily form varies less, be difficult to be observed according to formalness into
Row judgement, for Northeast Female tiger post-coitum whether successful pregnancy, effective method not yet in the short time.
Summary of the invention
It is an object of the invention to provide the methods that a kind of pair of Northeast Female tiger carries out super early pregnancy detection, make the raiser of northeastern tiger
No longer have no according to or only according to the variation of the tigress bodily form come blindly assume Northeast Female tiger gestation whether, to greatly mention
The early pregnancy judging nicety rate of high Northeast Female tiger contributes for the Reproduction of tiger with protection.
The technical solution of the invention is as follows:
A kind of super early pregnancy detection method of Northeast Female tiger, it the following steps are included:
Step 1: the freshly voided urine of continuous acquisition Northeast Female brave mating season, 1 time a day, differentiation and label are carried out, subzero 20 DEG C
Freezen protective；
Step 2: after to Northeast Female tiger mating, then the 1st week freshly voided urine after continuous acquisition its mating, often
It 1 time, carry out differentiation and label, subzero 20 DEG C of freezen protectives；
Step 3: the collected whole urine preparings of above two step are measured the progesterone level in each urine sample and are carried out
Record；
Step 4: the 1st week urine progesterone level surveyed after mating season and mating is carried out pair using SPSS statistical software
Than analysis, if numerical value meets normal distribution, independent sample T inspection is carried out respectively and is made a record；If numerical value belong to it is non-just
State distribution, then carry out Kruskal-Wallis H and Mann-Whitney U test and examine and make a record；
If it is average to be higher than the urine progesterone of mating season for the 1st week urine progesterone average level after Northeast Female tiger mating
Level, and reachPThe significance degree of < 0.05, so that it may judge this Northeast Female tiger successful pregnancy.
The solution have the advantages that: this method can make artificial feeding Captive Population be more convenient for managing, and no longer need
Tiger is allowed to carry out the secondary injured risk of fighting to avoid male and female tiger that mates, it is no longer necessary to tigress whether again heat is paid close attention to,
And better eating condition can be provided to determine the tigress of gestation, it is no longer necessary to judge that tigress is according to formalness observation
No successful pregnancy judges that tigress successful pregnancy effect is accurate.It is not only the morning of this I grades of country of northeastern tiger protection animal in imminent danger
Phase cyesiognosis and captive breeding management provide great convenience, also to the protection of northeastern tiger and other treasure the early stage of animals on the brink of extinction
Gestation judgement has good reference.
In addition, the method for this carly fruit drop Northeast Female tiger successful pregnancy is at low cost, speed is fast, and a urine specimen is sent
Inspection expense only has 10-12 member (to detect valence with the radioimmunoassay isotope method of Co., Ltd, Beijing North Institute of Biological Technology
For lattice)；1st week whole totally 14, sample after one Northeast Female brave about 7 days or so mating season, mating season and mating
Left and right, such 150-200 member or so can determine whether tiger is pregnant；It can provide within time general 3-5 days testing result, 1
In week can definitive result, there is good practical operability.
The present invention is the 1st week method for carrying out pregnant detection, inspection after one kind can mate to Northeast Female tiger
It is accurate to survey result, high reliablity.Raising and breeding manager no longer need will also moment sight incessantly after tiger mating
Whether can because do not have successful pregnancy and again heat, observing time generally requires not wait (female east in several thoughtful 1-2 months if examining tiger
The inter oestrual period time is usually 1 month twice in the northern nogestational situation of tiger)；No longer because observation misjudgment make to have succeeded it is pregnant
The tiger being pregnent mates again, and male and female tiger is caused tiger injury caused by fighting occur because of pairing discord.The present invention is the breeding management of tiger
Great convenience is provided, also provides reference for the cyesiognosis of other animals and breeding management.
Specific embodiment
One, experimental subjects:
12 Adult female northeastern tigers (have breeding to undergo), number be respectively as follows: No. 202, No. 262, No. 312, No. 382,
No. 440, No. 520, No. 526, No. 536, No. 576, No. 654, No. 754, No. 1154.
Two, experimental method:
It the following steps are included:
Step 1: the acquisition above-mentioned each stud mating phase of 12 Adult female northeastern tigers, (the Northeast Female tiger having was only involved in
Primary mating, some participation multiple matings, the Northeast Female tiger for participating in multiple heat mating select its last time mating season)
Freshly voided urine, 1 time a day (if failed to collect certain 1 day for some reason or several days urines, then the time postpones), each 2ml is carried out
It distinguishes and marks, be frozen in subzero 20 DEG C of refrigerator；
Step 2: after to Northeast Female tiger mating, then the 1st week freshly voided urine after continuous acquisition its mating, often
It 1 time (if failed to collect certain 1 day for some reason or several days urines, then the time postpones), each 2ml carries out differentiation and label, cold
Freeze in subzero 20 DEG C of refrigerator；
Step 3: by each Northeast Female tiger entire mating season, (the Northeast Female tiger of multiple heat mating selects the last of it
Mating season) and mating after urine preparing collected by the 1st week, when using radioimmunoassay isotope method to two
Progesterone level in phase whole Northeast Female tiger urine is measured and is made a record, and respectively obtains the result of Tables 1 and 2.
The urine progesterone level (ng/mL) of 1 Northeast Female of table brave mating season
Table 1(is continuous) the urine progesterone level (ng/mL) of Northeast Female brave mating season
Table the 1st week urine progesterone level (ng/mL) of 2 Northeast Female tiger post-coitum
Table 2(is continuous) the 1st week urine progesterone level (ng/mL) of Northeast Female tiger post-coitum
Step 4: the 1st week progesterone level surveyed utilizes SPSS system after mating season and mating to every Northeast Female tiger
Meter software compares and analyzes respectively, if data fit normal distribution, carries out independent sample T inspection respectively and makes a record；
If data belong to Non-Gaussian Distribution, carries out Kruskal-Wallis H and Mann-Whitney U test and examine and do
Record easy to remember.All statistics all carry out two-tailed test, level of significance α=0.05 using SPSS software, and average data is recorded as average
Value ± standard error (Mean ±SE)。
Whether being farrowed according to final Northeast Female tiger, it is pregnant and two groups not pregnant to be divided into, according to the urine of above-mentioned Tables 1 and 2
Progesterone data statistics goes out gestation group and non-gestation group mating season and post-coitum the 1st week urine progesterone average level makes a record,
Obtain the result of table 3 and table 4.
Otherness (the ng/ of urine progesterone average level in 3 pregnant female northeastern tiger mating season of table and post-coitum the 1st week
ML)
The otherness (ng/mL) of not pregnant the 1st week urine progesterone average level of brave mating season and post-coitum of table 4
By two above table, by the 1st week after every Northeast Female tiger acquisition mating urine progesterone average level and hand over
Urine progesterone average level with the phase compares, if the 1st week urine progesterone average level is significant after mating (P<
0.05) it is higher than the urine progesterone level of mating season, so that it may judge the Northeast Female tiger successful pregnancy, otherwise explanation is failed pregnant
It is pregnent.
Three, experimental result statisticallys analyze:
The progesterone average level such as table of 1st week urine after the Northeast Female of pregnant (post-coitum farrowing) brave mating season and mating
Shown in 3, the 1st week after mating, rising all occurs in the average level of progesterone in the Northeast Female tiger urine of all Pregnancy Success
Height, and it is this be raised through statistics reached it is significant (P< 0.05) or extremely significant (P< 0.01) it is horizontal.
The progesterone of the 1st week urine is average after the Northeast Female of not pregnant (post-coitum is not farrowed) brave mating season and mating
It is horizontal as shown in table 4, the 1st week after mating, the average level of progesterone in the Northeast Female tiger urine of all failed gestation
All be not apparent from raising (P> 0.05), or even there is the average progesterone level of two individuals (No. 262 and No. 382) decline occur.
Four, experimental result is summarized:
This method is a kind of feasible method for judging Northeast Female tiger extreme early gestation, if the after the mating of Northeast Female tiger
When 1 week urine progesterone average level is significantly higher than the urine progesterone average level of mating season, that is, it is pregnant to can determine whether that it has succeeded
It is pregnent.Not can determine whether the objective scientific method of gestation, the method at tiger post-coitum the 1st week still before this will be
The extreme early cyesiognosis and captive breeding management of this I grades of country of northeastern tiger protection animal in imminent danger provide great convenience, to it
Its kind tiger and other early pregnancy judgements for treasuring animals on the brink of extinction also have good reference.
Claims (5)
1. a kind of super early pregnancy detection method of Northeast Female tiger, it is characterised in that: it the following steps are included:
Step 1: the freshly voided urine of continuous acquisition Northeast Female brave mating season, 1 time a day, differentiation and label are carried out, subzero 20 DEG C
Freezen protective；
Step 2: after to Northeast Female tiger mating, then the 1st week freshly voided urine after continuous acquisition its mating, often
It 1 time, carry out differentiation and label, subzero 20 DEG C of freezen protectives；
Step 3: the collected whole urine preparings of above two step are measured the progesterone level in each urine sample and are carried out
Record；
Step 4: the 1st week urine progesterone level surveyed after mating season and mating is carried out pair using SPSS statistical software
Than analysis, if numerical value meets normal distribution, independent sample T inspection is carried out respectively and is made a record；If numerical value belong to it is non-just
State distribution, then carry out Kruskal-Wallis H and Mann-Whitney U test and examine and make a record；
If it is average to be higher than the urine progesterone of mating season for the 1st week urine progesterone average level after Northeast Female tiger mating
Level, and reachPThe significance degree of < 0.05, so that it may judge this Northeast Female tiger successful pregnancy.
2. the super early pregnancy detection method of Northeast Female tiger as described in claim 1, it is characterised in that in the first step, such as fail for some reason
Collect certain 1 day or several days urines, then the time postpones.
3. the super early pregnancy detection method of Northeast Female tiger as described in claim 1, it is characterised in that in second step, such as fail for some reason
Collect certain 1 day or several days urines, then the time postpones.
4. the super early pregnancy detection method of Northeast Female tiger as described in claim 1, it is characterised in that in third step, measure in urine
The method of progesterone level be radioimmunoassay isotope method.
5. the super early pregnancy detection method of Northeast Female tiger as described in claim 1, it is characterised in that in the 4th step, all statistics are all
Using two-tailed test, level of significance α=0.05, average data is recorded as average value ± standard error.
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|---|---|---|---|---|
| CN101995470A (en) * | 2009-08-31 | 2011-03-30 | 万积成 | Colloid gold immunochromatographic assay diagnosis method for early gestation of sows and detector |
| CN102735852A (en) * | 2012-06-12 | 2012-10-17 | 成都大熊猫繁育研究基地 | Determination method of giant panda estradiol true peak value |
| CN103207279A (en) * | 2013-03-21 | 2013-07-17 | 成都大熊猫繁育研究基地 | Quick panda progesterone concentration determination method |
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