CN102735852A - Determination method of giant panda estradiol true peak value - Google Patents
Determination method of giant panda estradiol true peak value Download PDFInfo
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Abstract
The invention discloses a determination method of a giant panda estradiol true peak value. According to the invention, urine samples of female giant pandas at a spring breeding period are collected and are subjected to chemical analysis; conditions such as a time for an estradiol maximal peak or a subsequent estradiol peak to decrease with an amplitude reaching a value above 50%, whether a progesterone value is in an increasing trend during the decreasing period of the estradiol maximal peak or the subsequent estradiol peak, whether the giant pandas show a typical estrous peak behavior, and a ratio of the corresponding progesterone value of the estradiol maximal peak or the subsequent estradiol peak to a progesterone basic value are combined, and the giant panda estradiol true peak value is determined. The method provided by the invention is obtained on the basis of statistical analysis of estradiol variation regularities of 78 oestrus giant pandas which constructs a maximal datum quantity currently, such that the method has high reliability. The method assists in filling up a giant panda research vacancy, and provides a scientific basis for improving captive giant panda artificial insemination conception rate.
Description
Technical field
The present invention relates to a kind of in good time test-tube basis for estimation of captive animal that is used for, particularly relate to a kind of determination methods of giant panda estradiol real peak.
Background technology
Giant panda is the distinctive rare and endangered wild animal of China; Himself fertility is on the low side, and female giant panda 1 year is oestrus for once, accepts natural mating and have only 1～3 day in oestrus; Its ovulation period possibly have only one day; And Captive Giant Panda natural mating ratio is on the low side, has only the giant panda about 50% can natural mating at present, has the giant panda about half to accomplish breeding through artificial insemination; So, confirm that giant panda breeding in good time in good time opportunity is the gordian technique that giant panda breeds.
Before the mid-90 in last century; The judgement that giant panda is bred opportunity is in good time mainly observed according to the giant panda determination of the nature of the act of oestrusing; Confirm that by rule of thumb giant panda breeds opportunity in good time; Cause the in good time artificial insemination time of giant panda is held unclear, the lacking quantitative index, this also is the major reason that causes the Giant Panda in Reproduction inefficiency.
After the mid-90 in last century; Through monitoring giant panda urine in oestrus estrogen Changing Pattern; Utilize the estrogen index to begin to be applied in the giant panda artificial insemination gradually as the index that giant panda breeds in good time; But, still there is not unified viewpoint to adopting the estrogen index to have very big dispute as the in good time test-tube index of giant panda.
The in good time artificial insemination viewpoint of giant panda that is proposed at present all proposes based on estrogen peak value time of occurrence; Because giant panda heat hormone change curve is originally as the undulatory property curve; A plurality of peak values can appear; And other a plurality of factors such as the giant panda ovulation also relates to progestational hormone, the behavior of oestrusing are for how judging definitely whether the estrogen peak value is that real peak does not still have any relevant report.
Summary of the invention
The technical matters that the present invention will solve is a kind ofly to carry out the determination methods that in good time artificial insemination provides the giant panda estradiol real peak of criterion for giant panda.
For solving the problems of the technologies described above, the determination methods of a kind of giant panda estradiol of the present invention real peak may further comprise the steps:
(1) breed spring during, by the time requirement female giant panda is carried out A urine sample collection device, observe the giant panda behavior of oestrusing simultaneously, after the urine sample marked, carry out freezing or stored refrigerated, adopt EUSA to detect estradiol value and progesterone value to urine sample then; The said mark that is recorded on the urine sample comprises that mainly female giant panda exhales name, urination time etc.; Described estradiol value and progesterone value are meant estradiol and the progesterone concentration value size in urine sample; Said estradiol is a kind of estrogen main in the urine sample, and said progesterone is a kind of progestational hormone main in the urine sample;
(2) with the giant panda urination time as horizontal ordinate, with estradiol value and the planimetric coordinates of progesterone value as ordinate on; Mark urination time and corresponding estradiol value and the determined two groups of coordinate points of progesterone value thereof respectively; Two groups of coordinate points are connected in turn respectively; Draw out estradiol value change curve and progesterone value change curve, find out estradiol peak-peak and corresponding progesterone value thereof; Described estradiol peak-peak also is the mxm. of estradiol;
(3) calculate the female giant panda progesterone basic value of oestrusing according to the progesterone value before the estradiol peak-peak time of occurrence, and calculate estradiol peak-peak or the progesterone value of the correspondence of estradiol peak value thereafter and the ratio of progesterone basic value;
(4) according to estradiol value change curve and progesterone value change curve; Whether in rising trend estradiol peak-peak or estradiol peak value thereafter reached time used more than 50%, estradiol progesterone value from the process that peak-peak or peak value are thereafter descending and giant panda in fall the oestrus pairing progesterone value of peak behavior, estradiol peak-peak or estradiol peak value thereafter and the situation such as ratio of progesterone basic value of typical case whether occur and combine simultaneously, judge giant panda estradiol real peak jointly; The said moment that giant panda estradiol real peak occurs is corresponding giant panda urination time.
The determination methods of described a kind of giant panda estradiol real peak; If estradiol peak-peak fall in 10 hours～20 hours scopes reaches 50% or more, afterwards estradiol drop to basic value, estradiol peak-peak correspondence continuously the ratio of progesterone value and progesterone basic value greater than 2; Simultaneously typical case's peak behavior of oestrusing appears in rising trend and giant panda in the corresponding progesterone value of estradiol estradiol peak-peak from the process that peak-peak descends, and can confirm that then this estradiol peak-peak is a panda estradiol real peak.
The determination methods of described a kind of giant panda estradiol real peak; If estradiol peak-peak fall in 20 hours～31 hours scopes reaches 50% or more, afterwards estradiol drop to basic value, estradiol peak-peak correspondence continuously the ratio of progesterone value and progesterone basic value greater than 1.8; Simultaneously typical case's peak behavior of oestrusing appears in rising trend and giant panda in estradiol progesterone value from the process that peak-peak descends, and can confirm that then this estradiol peak-peak is a giant panda estradiol real peak.
The determination methods of described a kind of giant panda estradiol real peak; If estradiol from peak-peak descend time of 50% more than 20 hours, the ratio of the corresponding progesterone value of estradiol peak-peak and progesterone basic value is less than 1.8; Occur simultaneously the not in rising trend and giant panda of the corresponding progesterone value of one or more peak values and estradiol estradiol peak-peak from the process that peak-peak descends after the estradiol peak-peak and typical case's peak behavior of oestrusing do not occur, can confirm that then the estradiol peak-peak is a false peaks; At this moment; Giant panda estradiol real peak is a certain peak value after the estradiol peak-peak, and this peak value must possess simultaneously that fall reaches 50% or more in 10 hours～20 hours scopes, afterwards the estradiol ratio that drops to the corresponding progesterone value of basic value, this peak value and progesterone basic value continuously typical case's situation such as peak behavior of oestrusing appear in rising trend and giant panda greater than 2, in the estradiol progesterone value that this peak value is corresponding from the process of this peak value decline.
Described 10 hours～20 hours scopes are meant that the giant panda estradiol is from the descend main criterion of 50% used time of real peak; It is to sum up in the statistics through 78 routine giant panda heat glycol Changing Patterns; The data volume of 78 examples is maximum in the world up to now data volume considerably beyond any report amount in the past, 80% giant panda individuality is arranged in 78 examples all in this scope; The criterion of therefore, 10 hours～20 hours scopes has extreme high reliability.
The described giant panda typical case peak behavior of oestrusing comprises that (the special behavior of oestrusing appears to the reflection of tangible pressure tail in the peak period giant panda oestrusing; Promptly stimulate the giant panda rear quarters with bamboo pole, the quiet end reaction that stands, sticks up appears in giant panda) and behavior to be joined (quiet stand, stick up tail, trunk sinkage independently appear in giant panda).
The determination methods of described a kind of giant panda estradiol real peak, its calculating of oestrusing female giant panda progesterone basic value is carried out according to the following steps:
1. choose all preceding progesterone values of giant panda estradiol peak-peak, find out the maximal value A in the selected progesterone value, calculate arithmetic mean value B, the standard deviation C of selected progesterone value, draw B+2C=D then;
If 2. A-D is for negative, the arithmetic mean value B of this moment is the progesterone basic value;
If 3. A-D then finds out all the progesterone values greater than D for just from the progesterone value of choosing, from residue progesterone value, find out maximal value A by the mode of 1. step
1, calculate the arithmetic mean value B that remains the progesterone value
1, standard deviation C
1, draw B then
1+ 2C
1=D
1
If 4. A
1-D
1Still for just, then repeat the 1. mode of step, up to A
n-D
nFor negative, the arithmetic mean value B of this moment
nBe the progesterone basic value, wherein n represents natural number.
The determination methods of described any giant panda estradiol real peak, it is following to the process that female giant panda carries out A urine sample collection device by the time requirement:
(1) observes female giant panda external genital, if after redness appears in external genital, then collect 1 part of female giant panda urina sanguinis every day;
(2) 3 parts of urine samples were collected in the beginning in the 4th day after rising continuously from estradiol every day at interval in about 8 hours;
(3) before and after female giant panda oestrused the peak behavior, every day is 5 parts of urine samples of collection in about 5 hours at interval;
(4) in case monitor the estradiol value downtrending is arranged, A urine sample collection device is shortened to about 3 hours interval time.
The determination methods of described any giant panda estradiol real peak, its each time the giant panda A urine sample collection device may further comprise the steps:
(1) cleans and dry beastly house hardened ground;
(2) giant panda that will collect urine sample is put into beastly house, and the staff waits for, in case giant panda is discharged urine, immediately giant panda is put into other beastly house;
(3) gather the fresh urine sample in ground with clean injectors, the freeze pipe of packing into, marked is put into freezing or cold storage plant then immediately.
Available data does not have any report and correlation technique and criterion about how judging giant panda estrogen real peak; The present invention will solve real peak how to judge the giant panda estradiol; Confirm criterion simultaneously, and the giant panda urination time that the estradiol real peak is corresponding is confirmed as the time of occurrence of estradiol real peak.
The giant panda urine sample gathering can directly have influence on the judgement to peak value interval time, and interval time, real peak value was missed in long meeting, and too short one side can increase workload and to the interference of animal, can reduce the feasibility of sampling on the other hand interval time.Uncertain and the disunity of giant panda urine sample gathering time of available data report, the acquisition time that has reaches 1 day at interval, can cause the error in judgement scope of time to peak bigger; And the sampling time interval scheme that the present invention adopts is about 3 hours in the peak period interval of oestrusing; Error in judgement scope to giant panda estradiol time to peak was narrowed down to about 1.5 hours, dwindled error in judgement greatly giant panda estradiol time to peak.
Existing public reported all detects the urine estrogen level merely to the judgement time of giant panda estrogen peak value, does not comprehensively judge and detect oestrus peak period progesterone level and behaviouristics.Judge the key whether estrogen real peak occurs is to judge whether estrogen real decline occurs, descend, actually or can not judge still that the estrogen fluctuation is real descends in case estrogen appears in monitoring.In order to address this problem one aspect of the present invention monitoring urine progesterone level in monitoring urine estradiol, confirm to quantize the true standard that descends of estradiol simultaneously, adopt the behaviouristics supplementary means to judge comprehensively whether giant panda urine estradiol is true decline.
Judge that the key whether estradiol real peak occurs is to judge whether estradiol real decline occurs; The present invention will confirm that the decline rate reference range of giant panda estradiol peak value quantizes to confirm whether the giant panda estradiol true decline occurs through statistical study giant panda heat as much as possible glycol data.
Suspicious peak value occurs in case monitor giant panda, through strengthening sampling density the time in SI is shortened to continuous sampling about 3 hours, the speed that the monitoring estradiol descends confirms whether estradiol is true decline.
Chengdu Panda Breeding Research Base has carried out statistical study to amounting to 78 routine giant panda estradiol Changing Patterns for many years; The analysis estradiol descends from real peak, and the required shortest time distributes more than 50%; Draw out oestrus as shown in Figure 1 female giant panda estradiol from the real peak 50% used time distribution frequency that descends; Adopt method of percentiles to confirm the giant panda urine estradiol peak value 50% used time reference value scope that descends, the result sees table 1:
Table 1. estradiol real peak 50% required time (h) that descends
Find by statistics as can be seen from Table 1 78 routine giant panda heat glycol Changing Patterns; Giant panda urine estradiol is that 31.17 hours, minimum of a value are 5.83 hours from the descend maximum of 50% required time of real peak; Wherein 80% giant panda individuality is all in 9.83～19.86 hours scopes; Promptly in 10～20 hours scopes, less than 20 hours; Analyze 16 routine giant pandas that the estradiol false peaks occurred simultaneously, analyze descend 50% required time and time thereof of estradiol peak value to distribute, the results are shown in Table 2:
Table 2. estradiol false peaks 50% required time (h) that descends
Can see from table 2; For the individuality that the estradiol false peaks occurs; Estradiol descends from false peaks, and will to be longer than the real peak value individual for 50% required time, and in 14.58～41.5 hours scopes, wherein 80% individuality is in 20～38.7 hours scopes; Promptly in 20～39 hours scopes, greater than 20 hours.
Utilize these data to judge that to differentiating giant panda urine estradiol is that fluctuation or true decline provide strong quantification reference data; In case monitoring giant panda urine estradiol occurs descending rapidly from a high value at short notice; If the estradiol fall reaches more than 50% in 10 hours～20 hours scope, then can judge this and reduce to true decline; And in a single day it is slow excessively the estradiol decline rate to occur, and the time of decline 50% surpasses 20 hours, can tentatively be judged as the estradiol fluctuation, but not true decline.
In the in good time test-tube public reported, only adopt giant panda estrogen peak value as a reference about giant panda existing, do not monitored the urine sample progesterone level simultaneously.The present invention monitors giant panda urine sample progesterone level, in case the decline of estradiol occurs simultaneously when detecting giant panda urine sample estradiol; Check that simultaneously progesterone level changes; If progesterone does not have rising trend, then be judged as the fluctuation of estradiol, but not true decline; If progesterone occur ascendant trend then estradiol true decline possibly appear; With the acquisition interval time that further shortens urine sample to per about 3 hours once, till the true fall time of full confirmation estradiol, thereby improved the accuracy that estradiol detects.
In order to quantize to confirm giant panda corresponding progesterone level difference when true and false estradiol peak value occurring, confirm the estradiol real peak in order to further quantification, compared the difference of the ratio of corresponding progesterone value of true and false estradiol peak value and progesterone basic value, the result sees table 3:
The progesterone value that the true and false estradiol peak value of table 3. is corresponding and the odds ratio of progesterone basic value are
Can see that from table 3 80% individuality is when the estradiol real peak occurring, its corresponding progesterone value level raises, and the ratio of progesterone value and its basic value is between 1.79～3.14, promptly greater than 1.8; And after false estradiol peak value occurring, its corresponding progesterone value level is lower, and 80% the individual progesterone value and the ratio of its basic value are between 0.94～2.13, and promptly less than 2, these data can quantize further to confirm whether estradiol is real peak.After if suspicious estradiol peak value occurring; Calculate the ratio of its corresponding progesterone value and progesterone basic value; If can confirm further that greater than 2 this estradiol peak value is a real peak; If this ratio is less than 1.8, then this estradiol peak value possibly be false peaks, if need further to combine the behaviouristics data to confirm greater than 1.8.
In the existing public reported; Whether behavior auxiliary judgment estrogen is the true relevant report that descends less than oestrusing about the employing giant panda; Only publish Science Press in 2006, the book number of the edition is p257, the author for Zhang Zhi and with " giant panda is protected theory and practice with moving " of Wei Fuwen in related description is arranged: the time of origin of 86.4% (19/22) giant panda natural mating first is after the estrogen peak value, and the time that giant panda can carry out natural mating appears at the peak of oestrusing usually.Should " giant panda protect theory and practice with moving " show to assist the employing giant panda peak behavior whether to occur oestrusing as the estrogen auxiliary reference foundation of decline whether truly.
In a single day the present invention monitors giant panda and estradiol decline occurs; Progesterone rises; Observe the giant panda behavior of oestrusing simultaneously, if typical case's peak behavior of oestrusing appears in giant panda, but then the auxiliary judgment giant panda estradiol occurs and truly descends; Otherwise still can not full confirmation, this will further get rid of estradiol and progesterone and occur the situation that fluctuates simultaneously.
Because in a single day giant panda heat glycol presents fluctuation formula change curve, and the estradiol peak value occurs, its decline rate is very fast; Because impossible continuous acquisition urine sample on giant panda; There is certain time interval in sampling, although strengthened SF, still possibly miss real estradiol peak value sample; Cause a part cognition situation of real estradiol peak value to occur but being not necessarily, false peaks occurs from the estradiol peak-peak that numerical value is seen.In existing all public reported, all less than about how rejecting the technology contents of false peaks.
Because the present invention has carried out statistical study to 78 example giant panda estradiol Changing Patterns in oestrus of present maximum amount of data; Having drawn three kinds judges giant panda estradiol real peak very reliably and goes out the method for now; Wherein the individual urine estradiol of 80% giant panda is 10 hours～20 hours from the real peak 50% required time range that descends; 80% individuality is when the estradiol real peak occurring; Its corresponding progesterone value level raises, and the ratio of progesterone value and its basic value is greater than 1.8; And after the estradiol false peaks occurring; Its corresponding progesterone value level is lower, and the progesterone value of 80% individuality and the ratio of its basic value is less than 2, with these several time ranges as main criterion; In order to quantize definite giant panda estradiol real peak and to go out now; Have extreme high reliability, filled up the blank of giant panda research field, scientific basis is provided for improving Captive Giant Panda artificial insemination pregnancy rate.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.
Fig. 1 for oestrus of the present invention female giant panda estradiol from the real peak 50% used time distribution frequency synoptic diagram that descends;
Fig. 2 is the individual first kind of estradiol value of the female giant panda of the present invention and the change curve of progesterone value;
Fig. 3 is the individual second kind of estradiol value of the female giant panda of the present invention and the change curve of progesterone value;
Fig. 4 is the third estradiol value of the female giant panda individuality of the present invention and the change curve of progesterone value.
Identify among the figure: α is the coordinate points of giant panda estradiol real peak on the estradiol value change curve; β is the coordinate points corresponding with α on the progesterone value change curve; Ec ng/mg Cr is expressed as the estradiol concentration value that the creatinine assay method obtains, and Pg ng/mg Cr is expressed as the progesterone concentration value that the creatinine assay method obtains.
Embodiment
The present invention observes female giant panda vulva color every day during breed spring; The characteristic performance that giant panda vulva redness starts for giant panda oestruses; In case find that vulva is red and swollen; Can confirm tentatively that giant panda gets into oestrus, continue to observe every day the giant panda behavior of oestrusing, if frequent marking behavior occurs, baa can confirm then that behavior giant panda oestruses.
After giant panda oestruses and starts; Begin female giant panda urine sample is collected; The record panda exhales name, urination time, typical case's marks such as peak behavior of oestrusing whether occur on urine sample; And in time the giant panda urine sample of collecting is carried out assay, adopt EUSA to detect and exhale name and corresponding estradiol value and the progesterone value of urination time with giant panda; Originally collect 1 part of urina sanguinis every day; 3 parts of urine samples were collected in beginning in the 4th day every day after the estradiol value rose continuously, and about 8 hours at interval, the time was respectively 7:00, and 15:00 is about 23:00; Oestrus before and after the peak, every day, urine sample was increased to about 5, and about 5 hours at interval, acquisition time was respectively: 6:00, and 11:00,16:00,21:00 is about 1:00; In case monitor the estradiol value downtrending is arranged, A urine sample collection device is shortened to about 3 hours interval time, till the true decline of final affirmation estradiol value.
The giant panda A urine sample collection device all is that hardened ground after the cleaning is collected each time, after cleaning earlier and drying beastly house ground, need is collected the giant panda of urine sample and is put into beastly house; The staff waits for then, in case giant panda is discharged urine, immediately giant panda is put into other beastly house, gathers the fresh urine sample in ground with clean injectors, the freeze pipe of packing into, and marked is put into freezing preservation of freezer compartment of refrigerator or reefer stored refrigerated immediately.
With the giant panda urination time as horizontal ordinate, with estradiol value and the planimetric coordinates of progesterone value as ordinate on; Mark urination time and corresponding estradiol value and the determined two groups of coordinate points of progesterone value thereof respectively; Two groups of coordinate points are connected in turn respectively; Draw out estradiol value change curve and progesterone value change curve, find out the corresponding progesterone value of estradiol peak-peak or estradiol peak value thereafter, calculate the female giant panda progesterone basic value of oestrusing.
The calculating of the female giant panda progesterone basic value of oestrusing is carried out according to the following steps:
1. choose the preceding corresponding progesterone value of all giant panda estradiol values of giant panda estradiol peak-peak, find out the maximal value A in the selected progesterone value, calculate arithmetic mean value B, the standard deviation C of selected progesterone value, draw B+2C=D then;
If 2. A-D is for negative, the arithmetic mean value B of this moment is the progesterone basic value;
If 3. A-D then finds out all the progesterone values greater than D for just from the progesterone value of choosing, from residue progesterone value, find out maximal value A by the mode of 1. step
1, calculate the arithmetic mean value B that remains the progesterone value
1, standard deviation C
1, draw B then
1+ 2C
1=D
1
If 4. A
1-D
1Still for just, then repeat the 1. mode of step, up to A
n-D
nFor negative, the arithmetic mean value B of this moment
nBe the progesterone basic value, wherein n is a natural number.
According to estradiol value change curve and progesterone value change curve; Whether in rising trend estradiol peak-peak or estradiol peak value thereafter reached time used more than 50%, estradiol progesterone value from the process that peak-peak or peak value are thereafter descending and giant panda in fall the oestrus pairing progesterone value of peak behavior, estradiol peak-peak or estradiol peak value thereafter and the situation such as ratio of progesterone basic value of typical case whether occur and combine simultaneously, judge giant panda estradiol real peak jointly.The method of judging giant panda estradiol real peak has following three kinds of situation respectively.
First kind of situation: as shown in Figure 2; If estradiol peak-peak fall in 10 hours～20 hours scopes reaches 50% or more, afterwards estradiol drop to basic value, estradiol peak-peak correspondence continuously the ratio of progesterone value and progesterone basic value greater than 2; Simultaneously typical case's peak behavior of oestrusing appears in rising trend and giant panda in estradiol progesterone value from the process that peak-peak descends; Can confirm that then this estradiol peak-peak is a giant panda estradiol real peak; Be the α coordinate points among Fig. 2, β is the progesterone value coordinate points corresponding with α.
Second kind of situation: as shown in Figure 3; If estradiol peak-peak fall in 20 hours～31 hours scopes reaches 50% or more, afterwards estradiol drop to basic value, estradiol peak-peak correspondence continuously the ratio of progesterone value and progesterone basic value greater than 1.8; Simultaneously typical case's peak behavior of oestrusing appears in rising trend and giant panda in estradiol progesterone value from the process that peak-peak descends; Can confirm that then this estradiol peak-peak is a giant panda estradiol real peak; Be the α coordinate points among Fig. 3, β is the progesterone value coordinate points corresponding with α.
The third situation: as shown in Figure 4; If estradiol from peak-peak descend time of 50% more than 20 hours, the ratio of the corresponding progesterone value of estradiol peak-peak and progesterone basic value is less than 1.8; Occur simultaneously the not in rising trend and giant panda of one or more peak values and estradiol progesterone value from the process that peak-peak descends after the estradiol peak-peak and typical case's peak behavior of oestrusing do not occur, can confirm that then this estradiol peak-peak is a false peaks; At this moment; Giant panda estradiol real peak is a certain peak value after the estradiol peak-peak; And this peak value must possess simultaneously, and fall reaches more than 50% in 10 hours～20 hours scopes, afterwards the estradiol ratio that drops to the corresponding progesterone value of basic value, this peak value and progesterone basic value continuously typical case's situation such as peak behavior of oestrusing appear in rising trend and giant panda greater than 2, in the estradiol progesterone value that this peak value is corresponding from the process of this peak value decline; Be the α coordinate points among Fig. 4, β is the progesterone value coordinate points corresponding with α.
Be corresponding giant panda urination time the now that goes out of said giant panda estradiol real peak.
Claims (6)
1. the determination methods of a giant panda estradiol real peak may further comprise the steps:
(1) breed spring during, by the time requirement female giant panda is carried out A urine sample collection device, observe the giant panda behavior of oestrusing simultaneously, after the urine sample marked, carry out freezing or stored refrigerated, adopt EUSA to detect estradiol value and progesterone value to urine sample then;
(2) with the giant panda urination time as horizontal ordinate, with estradiol value and the planimetric coordinates of progesterone value as ordinate on; Mark urination time and corresponding estradiol value and the determined two groups of coordinate points of progesterone value thereof respectively; Two groups of coordinate points are connected in turn respectively; Draw out estradiol value change curve and progesterone value change curve, find out estradiol peak-peak time of occurrence and corresponding progesterone value thereof;
(3) calculate the female giant panda progesterone basic value of oestrusing according to the progesterone value before the estradiol peak-peak time of occurrence, and calculate estradiol peak-peak or the progesterone value of the correspondence of estradiol peak value thereafter and the ratio of progesterone basic value;
(4) according to estradiol value change curve and progesterone value change curve; Whether in rising trend estradiol peak-peak or estradiol peak value thereafter reached time used more than 50%, estradiol progesterone value from the process that peak-peak or peak value are thereafter descending and giant panda in fall the oestrus pairing progesterone value of peak behavior, estradiol peak-peak or estradiol peak value thereafter and the situation such as ratio of progesterone basic value of typical case whether occur and combine simultaneously, judge giant panda estradiol real peak jointly.
2. the determination methods of a kind of giant panda estradiol real peak according to claim 1; It is characterized in that: if estradiol peak-peak fall in 10 hours～20 hours scopes reaches 50% or more, afterwards estradiol drop to basic value, estradiol peak-peak correspondence continuously the ratio of progesterone value and progesterone basic value greater than 2; Simultaneously typical case's peak behavior of oestrusing appears in rising trend and giant panda in estradiol progesterone value from the process that peak-peak descends, and can confirm that then this estradiol peak-peak is a giant panda estradiol real peak.
3. the determination methods of a kind of giant panda estradiol real peak according to claim 1; It is characterized in that: if estradiol peak-peak fall in 20 hours～31 hours scopes reaches 50% or more, afterwards estradiol drop to basic value, estradiol peak-peak correspondence continuously the ratio of progesterone value and progesterone basic value greater than 1.8; Simultaneously typical case's peak behavior of oestrusing appears in rising trend and giant panda in estradiol progesterone value from the process that peak-peak descends, and can confirm that then this estradiol peak-peak is a giant panda estradiol real peak.
4. the determination methods of a kind of giant panda estradiol real peak according to claim 1; It is characterized in that: if estradiol from peak-peak descend time of 50% more than 20 hours, the ratio of the corresponding progesterone value of estradiol peak-peak and progesterone basic value is less than 1.8; Occur simultaneously the not in rising trend and giant panda of one or more peak values and estradiol progesterone value from the process that peak-peak descends after the estradiol peak-peak and typical case's peak behavior of oestrusing do not occur, can confirm that then this estradiol peak-peak is a false peaks; At this moment; Giant panda estradiol real peak is a certain peak value after the estradiol peak-peak, and this peak value must possess simultaneously that fall reaches 50% or more in 10 hours～20 hours scopes, afterwards the estradiol ratio that drops to the corresponding progesterone value of basic value, this peak value and progesterone basic value continuously typical case's situation such as peak behavior of oestrusing appear in rising trend and giant panda greater than 2, in the estradiol progesterone value that this peak value is corresponding from the process of this peak value decline.
5. according to the determination methods of the described any giant panda estradiol real peak of claim 1～4, it is following to the process that female giant panda carries out A urine sample collection device by the time requirement:
(1) observes female giant panda external genital, if after redness appears in external genital, then collect 1 part of female giant panda urina sanguinis every day;
(2) 3 parts of urine samples were collected in the beginning in the 4th day after rising continuously from estradiol every day at interval in about 8 hours;
(3) before and after female giant panda oestrused the peak behavior, every day is 5 parts of urine samples of collection in about 5 hours at interval;
(4) in case monitor the estradiol value downtrending is arranged, A urine sample collection device is shortened to about 3 hours interval time.
6. the determination methods of a kind of giant panda estradiol real peak according to claim 5, its each time the giant panda A urine sample collection device may further comprise the steps:
(1) cleans and dry beastly house hardened ground;
(2) giant panda that will collect urine sample is put into beastly house, and the staff waits for, in case giant panda is discharged urine, immediately giant panda is put into other beastly house;
(3) gather the fresh urine sample in ground, the freeze pipe of packing into, marked, freezing immediately then or stored refrigerated with clean injectors.
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| CN103126783A (en) * | 2013-03-06 | 2013-06-05 | 成都大熊猫繁育研究基地 | Method for optimizing timely artificial insemination for pandas by aid of LH (luteinizing hormone) results |
| CN103126728A (en) * | 2013-03-06 | 2013-06-05 | 成都大熊猫繁育研究基地 | Determination method of normal or delayed ovulation in pandas |
| CN103207280A (en) * | 2013-03-21 | 2013-07-17 | 成都大熊猫繁育研究基地 | Method for predicting panda expected delivery date |
| CN108401916A (en) * | 2018-05-07 | 2018-08-17 | 中国大熊猫保护研究中心 | A kind of giant panda colony house |
| CN110514849A (en) * | 2019-08-24 | 2019-11-29 | 牡丹江师范学院 | A kind of super early pregnancy detection method of Northeast Female tiger |
| CN112083156A (en) * | 2020-09-18 | 2020-12-15 | 成都大熊猫繁育研究基地 | Prediction method of panda estrone peak time and application thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103126783A (en) * | 2013-03-06 | 2013-06-05 | 成都大熊猫繁育研究基地 | Method for optimizing timely artificial insemination for pandas by aid of LH (luteinizing hormone) results |
| CN103126728A (en) * | 2013-03-06 | 2013-06-05 | 成都大熊猫繁育研究基地 | Determination method of normal or delayed ovulation in pandas |
| CN103126728B (en) * | 2013-03-06 | 2014-03-19 | 成都大熊猫繁育研究基地 | Determination method of normal or delayed ovulation in pandas |
| CN103207280A (en) * | 2013-03-21 | 2013-07-17 | 成都大熊猫繁育研究基地 | Method for predicting panda expected delivery date |
| CN103207280B (en) * | 2013-03-21 | 2014-01-01 | 成都大熊猫繁育研究基地 | Method for predicting panda expected delivery date |
| CN108401916A (en) * | 2018-05-07 | 2018-08-17 | 中国大熊猫保护研究中心 | A kind of giant panda colony house |
| CN110514849A (en) * | 2019-08-24 | 2019-11-29 | 牡丹江师范学院 | A kind of super early pregnancy detection method of Northeast Female tiger |
| CN112083156A (en) * | 2020-09-18 | 2020-12-15 | 成都大熊猫繁育研究基地 | Prediction method of panda estrone peak time and application thereof |
| CN112083156B (en) * | 2020-09-18 | 2021-06-29 | 成都大熊猫繁育研究基地 | Prediction method of panda estrone peak time and application thereof |
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