CN101914483A - Liquid-based thin-layer cell preservation solution and use thereof - Google Patents
Liquid-based thin-layer cell preservation solution and use thereof Download PDFInfo
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- CN101914483A CN101914483A CN2010102707466A CN201010270746A CN101914483A CN 101914483 A CN101914483 A CN 101914483A CN 2010102707466 A CN2010102707466 A CN 2010102707466A CN 201010270746 A CN201010270746 A CN 201010270746A CN 101914483 A CN101914483 A CN 101914483A
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Abstract
The invention discloses liquid-based thin-layer cell preservation solution and use thereof. The preservation solution comprises 5 to 28 volume percent of aldehyde solution, 0.1 to 3 volume percent of acid solution, 0.1 to 6 mass volume percent of chloride, 0.1 to 3 mass volume percent of tris(hydroxymethyl)aminomethane and the balance volume percent of double distilled water. The preservation solution of the invention can remove red blood cells, preserve white blood cells, timely fix the original state of cells of diagnostic value, soften mucilage and epidermal tissues, weaken mucous effect and separate effective cells from mucilage. The microscopic examination piece manufactured by using the preservation solution has clear microscopic examination view field and high cellular staining contrast and is widely suitable for gynecological and non-gynecological exfoliative cytological specimens and manual, semi-automatic or fully-automatic liquid-based cell substrate making processes. The production cost of the preservation solution is low, so the application and promotion of a liquid-based thin-layer cell detection technique are convenient.
Description
Technical field
The present invention relates to a kind of cell treatment solution, relate in particular to a kind of liquid-based thin-layer cell preservation solution and uses thereof.
Background technology
Flaking method commonly used in the cell detection is Pasteur's cell smear method, and the doctor needs manual will being coated on the microgram with the cell sample that collector is obtained.The smear that this traditional-handwork method is made has only been collected maximum 20% cell, and the cell sample more than 80% then remains on the sampling thief and with sampling thief and is dropped.In addition, be coated with sector-meeting because of the influence of blood, mucus and inflammation tissue become muddiness and fixing untimely caused artefact more than 40%.These shortcomings are to have caused the inaccurate reason of most conventional Pasteur test result.
Liquid based thin-layer cell method of tableting is the advanced in the world cytolgical examination technology that grew up in recent years, different with conventional Pasteur's cell smear method is, liquid based thin-layer cell method of tableting is to insert in the bottle that cell preservation treatment solution is housed having cast-off cells sample collecting device, preserve treatment solution by cell the cast-off cells sample is carried out rinsing, adopt manual, semi or fully automatic method with sample dispersion, filtration back film-making again, carry out microscopic inspection and diagnosis at last.Liquid based thin-layer cell method of tableting is particularly suitable for cervical exfoliated cell is carried out the cytology specification diagnosis, be that a kind of cervical cancer cell is efficiently learned the inspection technology, check that with Pasteur's cell smear of traditional cervical smear specific energy obviously improves the satisfactory degree of sample and the recall rate of abnormal cervical cell mutually.
At present, the cell of import is preserved treatment solution and is cost an arm and a leg, about 180 yuan of a not good liquor based thin-layer cell inspection charge, and high like this check fee makes that this technology is difficult in the low income area promote; In the domestic like product, as the liquid based thin-layer cell flaking method among the patent of invention ZL200610021857.7 " simplified thin-layer liquid-based cytology detection method ", its cell-preservation liquid and treatment solution separate, and the film-making process is loaded down with trivial details, and efficient is low.
Summary of the invention
The purpose of this invention is to provide a kind of liquid-based thin-layer cell preservation solution that uses for manual, semi-automatic or full-automatic cell pelleter and uses thereof.
Liquid-based thin-layer cell preservation solution of the present invention comprises that volume percent is the aldehydes solution of 5%-28%; And volume percent is the acids solution of 0.1%-3%; And the quality volume percent is the muriate of 0.1%-6%; And the quality volume percent is the Tutofusin tris of 0.1%-3%; Remaining volume fraction is supplied by distilled water.Described aldehydes solution be selected from formaldehyde solution, glutaraldehyde solution, paraformaldehyde solution, acrolein solution, in dialdehyde solution, the mda solution any one or more than one, the mass concentration of described aldehydes solution is 25%-40%.Described acids solution is selected from any one or two kinds of hydrochloric acid soln, glacial acetic acid, and in the acids solution, the mass concentration of hydrochloric acid soln is 36%-38%, and the mass concentration of glacial acetic acid is more than or equal to 99%.Described muriate be selected from Repone K, sodium-chlor, the calcium chloride any one or more than one.
The volume percent of acids solution is preferably 1% among the present invention.
Liquid-based thin-layer cell preservation solution is used for the processing to the sample that contains cast-off cells among the present invention, and the described sample that contains cast-off cells is that cervical secretions, dropsy of serous cavity, urine, sputum, cerebrospinal fluid, esophagus draw in the net, the segmental bronchus washing lotion.
Preservation treatment solution of the present invention can be removed red corpuscle, preserves white corpuscle, in time fixedly has the original form of the cell of diagnostic value; Mucus and face tissue are softened, dwindle the mucus effect, effective cell is separated from mucus; Cell is natural subsidence in preserving treatment solution, form individual layer tiling cell easily, the microscopy sheet that utilizes this preservation treatment solution to make has the microscopy visual field and good cell dyeing contrast gradient clearly, is widely used in exfoliative cytology sample and manual, semi or fully automatic liquid basal cell film-making processes such as various gynaecology and non-gynaecology.In addition, this production cost of preserving treatment solution is low, helps the application and the popularization of liquid based thin-layer cell detection technique.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is described in further detail:
The uterine neck normal squamous cell microscopy photo of Fig. 1 for handling through preservation treatment solution of the present invention;
The cervical lesions zone cell microscopy photo of Fig. 2 for handling through preservation treatment solution of the present invention.
Embodiment
Relational language among the present invention is described further:
Herein, term " volume percent " accounts for the per-cent of complete soln volume for the volume of solute, and it goes for liquid solute.
Herein, term " quality volume percent " is the quality of solute and the per-cent of complete soln volume, and it goes for solid solute, and mass unit is gram in the quality volume percent of this paper, and volume unit is a milliliter.
Herein, term " cast-off cells " also can be got or be scrubbed and obtain except that natural cast-off cells by artificial Qiao.
Liquid-based thin-layer cell preservation solution of the present invention comprises that volume percent is the aldehydes solution of 5%-28%; And volume percent is the acids solution of 0.1%-3%; And the quality volume percent is the muriate of 0.1%-6%; And the quality volume percent is the Tutofusin tris of 0.1%-3%; Remaining volume fraction is supplied by distilled water.Described aldehydes solution be selected from formaldehyde solution, glutaraldehyde solution, paraformaldehyde solution, acrolein solution, in dialdehyde solution, the mda solution any one or more than one, the mass concentration of described aldehydes solution is 25%-40%.Described acids solution is selected from any one or two kinds of hydrochloric acid soln, glacial acetic acid, and in the acids solution, the mass concentration of hydrochloric acid soln is 36%-38%, and the mass concentration of glacial acetic acid is more than or equal to 99%.Described muriate be selected from Repone K, sodium-chlor, the calcium chloride any one or more than one.
The volume percent of acids solution is preferably 1% among the present invention.
The mass concentration of aldehydes solution is 25%-40% among the present invention, can use the commercial reagent in public field, is not in 25%-40% as the mass concentration of reagent, can adopt conventional test method, and it is formulated in this scope.
In the acids solution, the mass concentration of hydrochloric acid soln is 36%-38% among the present invention, and the mass concentration of glacial acetic acid is more than or equal to 99%, and they can use the commercial reagent in public field.
Among the present invention, the preferred distilled water of water, it is through the resulting water of twice distillation.
Liquid-based thin-layer cell preservation solution of the present invention is used for the processing to the sample that contains cast-off cells, and the described sample that contains cast-off cells is that cervical secretions, dropsy of serous cavity, urine, sputum, cerebrospinal fluid, esophagus draw in the net, the segmental bronchus washing lotion.
The preparation method of liquid-based thin-layer cell preservation solution of the present invention is as follows: need to determine the preservation treatment solution cumulative volume of preparation, calculate also weighing or measure various components, these components are mixed the back stirring and dissolving can obtain this preservation treatment solution.
Embodiment 1:
According to the form below weighing or measure each component,
Mass concentration is 25% formaldehyde solution 50mL
Mass concentration is 36% hydrochloric acid soln 1mL
Repone K 1g
Tutofusin tris 1g
Distilled water 949mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 2:
According to the form below weighing or measure each component,
Mass concentration is 30% glutaraldehyde solution 280mL
Mass concentration is 36% hydrochloric acid soln 30mL
Sodium-chlor 60g
Tutofusin tris 30g
Distilled water 690mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 3:
According to the form below weighing or measure each component,
Mass concentration is 35% paraformaldehyde solution 100mL
Glacial acetic acid 20mL
Calcium chloride 10g
Tutofusin tris 10g
Distilled water 880mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 4:
According to the form below weighing or measure each component,
Mass concentration is 40% acrolein solution 175mL
Glacial acetic acid 25mL
Sodium-chlor 30g
Tutofusin tris 15g
Distilled water 800mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 5:
According to the form below weighing or measure each component,
Mass concentration is 28% the solution of dialdehyde 150mL
Mass concentration is 38% hydrochloric acid soln 15mL
Repone K 50g
Tutofusin tris 20g
Distilled water 835mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 6:
According to the form below weighing or measure each component,
Mass concentration is 33% mda solution 80mL
Glacial acetic acid 20mL
Calcium chloride 8g
Tutofusin tris 2g
Distilled water 900mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 7:
According to the form below weighing or measure each component,
Mass concentration is 38% formaldehyde solution 30mL
Mass concentration is 36% the solution of dialdehyde 30mL
Mass concentration is 37% hydrochloric acid soln 20mL
Repone K 22g
Tutofusin tris 26g
Distilled water 920mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 8:
According to the form below weighing or measure each component,
Mass concentration is 29% mda solution 70mL
Mass concentration is 35% acrolein solution 150mL
Glacial acetic acid 10mL
Mass concentration is 36% hydrochloric acid soln 10mL
Calcium chloride 12g
Sodium-chlor 15g
Tutofusin tris 20g
Distilled water 760mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 9:
According to the form below weighing or measure each component,
Mass concentration is 25% mda solution 70mL
Mass concentration is 40% paraformaldehyde solution 80mL
Glacial acetic acid 0.5mL
Mass concentration is 37% hydrochloric acid soln 0.5mL
Sodium-chlor 10g
Tutofusin tris 14g
Distilled water 849mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Embodiment 10:
According to the form below weighing or measure each component,
Mass concentration is 30% formaldehyde solution 25mL
Mass concentration is 25% paraformaldehyde solution 25mL
Glacial acetic acid 25mL
Mass concentration is 38% hydrochloric acid soln 5mL
Calcium chloride 10g
Tutofusin tris 30g
Distilled water 920mL
Each component is mixed the back stirring and dissolving, can obtain this preservation treatment solution.
Experimental example:
Adopt conventional gynecologial examination method, extract divide a word with a hyphen at the end of a line zone cast-off cells and use preservation treatment solution of the present invention (being prepared) that sample is handled of woman uterus by embodiment 3.Utilize full-automatic LCT liquid base film-making coloring system to carry out film-making and microscopy is taken pictures.More than all operation only be existing known technology.
Shown in Fig. 1 or 2, this microscopy sheet does not exist red corpuscle and mucous interference, microscopy gets a clear view and cell dyeing contrast gradient height, illustrates that liquid-based thin-layer cell preservation solution of the present invention has good treatment effect to sample, and reliable cell microscopy picture can be provided for the diagnosis of disease.
Claims (7)
1. liquid-based thin-layer cell preservation solution, it is characterized in that: it comprises that volume percent is the aldehydes solution of 5%-28%; And volume percent is the acids solution of 0.1%-3%; And the quality volume percent is the muriate of 0.1%-6%; And the quality volume percent is the Tutofusin tris of 0.1%-3%; Remaining volume integral is than being supplied by water.
2. preservation treatment solution according to claim 1, it is characterized in that: described aldehydes solution be selected from formaldehyde solution, glutaraldehyde solution, paraformaldehyde solution, acrolein solution, in dialdehyde solution, the mda solution any one or more than one, the mass concentration of described aldehydes solution is 25%-40%.
3. preservation treatment solution according to claim 1 is characterized in that: described acids solution is selected from any one or two kinds of hydrochloric acid soln, glacial acetic acid, and the mass concentration of described hydrochloric acid soln is 36%-38%, and the mass concentration of glacial acetic acid is more than or equal to 99%.
4. preservation treatment solution according to claim 1 is characterized in that: described muriate be selected from Repone K, sodium-chlor, the calcium chloride any one or more than one.
5. preservation treatment solution according to claim 1 is characterized in that: the volume percent of described acids solution is 1%.
6. the purposes of the described liquid-based thin-layer cell preservation solution of claim 1, it is characterized in that: it is used for the processing to the sample that contains cast-off cells.
7. the purposes of liquid-based thin-layer cell preservation solution according to claim 6 is characterized in that: the described sample that contains cast-off cells is that cervical secretions, dropsy of serous cavity, urine, sputum, cerebrospinal fluid, esophagus draw in the net, the segmental bronchus washing lotion.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091501A (en) * | 2011-10-28 | 2013-05-08 | 黄伏生 | Preparation method of liquid-based cell system quality control substance |
| CN103461320A (en) * | 2013-08-19 | 2013-12-25 | 安徽信灵检验医学科技有限公司 | Non-isotonic preserving fluid for liquid-based thin-layer exfoliative cells |
| CN103884549A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Sputum cell treatment liquid and application thereof |
| CN103875653A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Endocervical cell preserving fluid and method for preparing endocervical cell specimen |
| CN104745670A (en) * | 2015-04-16 | 2015-07-01 | 黄小军 | Preparation method of cell chip and application of cell chip in tumour screening field |
| CN105779559A (en) * | 2016-03-28 | 2016-07-20 | 天津智达康华生物科技有限公司 | Liquid-based cell treatment solution |
| CN106442078A (en) * | 2016-08-25 | 2017-02-22 | 四川金域医学检验中心有限公司 | Alkali solution based cell preservation treating fluid and preparation method thereof |
| CN107202888A (en) * | 2017-07-11 | 2017-09-26 | 广州江元医疗科技有限公司 | A kind of p16 for cervical liquid-based cellsINK4aImmune labeled colour reagent box |
| CN109612800A (en) * | 2019-01-09 | 2019-04-12 | 济南金域医学检验中心有限公司 | A kind of flaking method of non-gynecological liquid-based specimens |
| CN109668771A (en) * | 2019-02-26 | 2019-04-23 | 长沙协大生物科技有限公司 | A kind of liquid-based cast-off cells dyeing liquor and its colouring method |
| CN116649330A (en) * | 2023-07-17 | 2023-08-29 | 深圳市森盈智能科技有限公司 | Liquid-based exfoliated cell preservation liquid, kit and application |
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| CN101222927A (en) * | 2005-05-18 | 2008-07-16 | Mpex医药有限公司 | Aerosolized fluoroquinolones and uses thereof |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091501A (en) * | 2011-10-28 | 2013-05-08 | 黄伏生 | Preparation method of liquid-based cell system quality control substance |
| CN103091501B (en) * | 2011-10-28 | 2016-08-03 | 黄伏生 | Liquid basal cell mass of system controls the preparation method of thing |
| CN103461320A (en) * | 2013-08-19 | 2013-12-25 | 安徽信灵检验医学科技有限公司 | Non-isotonic preserving fluid for liquid-based thin-layer exfoliative cells |
| CN103461320B (en) * | 2013-08-19 | 2015-09-02 | 安徽信灵检验医学科技有限公司 | An a kind of conserving liquid such as not grade for liquid base thin layer cast-off cells |
| CN103875653B (en) * | 2014-02-11 | 2015-03-18 | 中国人民解放军北京军区总医院 | Endocervical cell preserving fluid and method for preparing endocervical cell specimen |
| CN103875653A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Endocervical cell preserving fluid and method for preparing endocervical cell specimen |
| CN103884549A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Sputum cell treatment liquid and application thereof |
| CN104745670A (en) * | 2015-04-16 | 2015-07-01 | 黄小军 | Preparation method of cell chip and application of cell chip in tumour screening field |
| CN105779559A (en) * | 2016-03-28 | 2016-07-20 | 天津智达康华生物科技有限公司 | Liquid-based cell treatment solution |
| CN106442078A (en) * | 2016-08-25 | 2017-02-22 | 四川金域医学检验中心有限公司 | Alkali solution based cell preservation treating fluid and preparation method thereof |
| CN106442078B (en) * | 2016-08-25 | 2019-09-10 | 四川金域医学检验中心有限公司 | A kind of alkalinity liquid basal cell saves treatment fluid and preparation method thereof |
| CN107202888A (en) * | 2017-07-11 | 2017-09-26 | 广州江元医疗科技有限公司 | A kind of p16 for cervical liquid-based cellsINK4aImmune labeled colour reagent box |
| CN109612800A (en) * | 2019-01-09 | 2019-04-12 | 济南金域医学检验中心有限公司 | A kind of flaking method of non-gynecological liquid-based specimens |
| CN109668771A (en) * | 2019-02-26 | 2019-04-23 | 长沙协大生物科技有限公司 | A kind of liquid-based cast-off cells dyeing liquor and its colouring method |
| CN116649330A (en) * | 2023-07-17 | 2023-08-29 | 深圳市森盈智能科技有限公司 | Liquid-based exfoliated cell preservation liquid, kit and application |
| CN116649330B (en) * | 2023-07-17 | 2024-03-01 | 深圳市森盈智能科技有限公司 | Liquid-based exfoliated cell preservation liquid, kit and application |
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Address after: 350000, Fuzhou, Fujian Province, Mawei District, 1 Confucian Road, the new mainland science and Technology Park (FTA test area) Patentee after: FUJIAN XILONG BIOTECHNOLOGY CO.,LTD. Address before: 350000 new world science park, 1 Confucian Avenue, Mawei District, Fujian, Fuzhou Patentee before: FUJIAN NEWLAND LIFE TECHNOLOGIES (HOLDINGS) Co.,Ltd. |
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