CN101664028B - Liquid based cell preservative fluid - Google Patents
Liquid based cell preservative fluid Download PDFInfo
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- CN101664028B CN101664028B CN 200910034575 CN200910034575A CN101664028B CN 101664028 B CN101664028 B CN 101664028B CN 200910034575 CN200910034575 CN 200910034575 CN 200910034575 A CN200910034575 A CN 200910034575A CN 101664028 B CN101664028 B CN 101664028B
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- liquid based
- based cell
- preservative fluid
- cell preservative
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Abstract
The invention discloses a liquid based cell preservative fluid which is used for matching with an imported or home-made film type liquid based cell film making machine for making films and belongs to the technical field of medicaments. The liquid based cell preservative fluid basically consists of the following components according to the ratios by volume: 10-30 percent of alcohols and the balance of double distilled water; the liquid based cell preservative fluid comprises the following components with the concentration expressed in percentage by mass/volume: 0.1-3.0 percent of phosphate, 0.1-2.0 percent of phenol and 0.1-2.0 percent of N-methyl-N-cyclohexyl-2-amino-3, 5-dibromobenzylamine. Firstly, the invention uses an ordinary biochemical reagent, and the price of consumptive materials is reduced in comparison with the cell preservative fluid produced by an imported auxiliary consumptive material Thinprer TCT. Secondly, because the N-methyl-N-cyclohexyl-2-amino-3, 5-dibromobenzylamine as a unique mucus decomposer is used, the cells wrapped by the mucus in a sample can be successfully separated, avoiding the accumulation and loss of positive cells. Smears prepared by using the liquid based cell preservative fluid to match with an automatic film making machine have clear and transparent backgrounds and uniformly distributed cell monolayers, and are convenient for the film reading of doctors in a pathology department.
Description
Technical field
The present invention relates to a kind of liquid based cell preservative fluid, support the use for import or domestic diaphragm type liquid foot cell pellet mill film-making.Belong to the medicament technical field.
Background technology
Cervical carcinoma is one of modal malignant tumour in present women's cancer.Early diagnosis cervical carcinoma and precancerous lesion are to improve the key of cervical carcinoma cure rate and survival, and cytolgical examination is the main method of widely examination cervix cancer of present clinical practice.It is a subject that changes to diagnose clinical disease with the observation of cell Structure and form.In decades cytolgical examination has become the main method of extensive generaI investigation and people at highest risk's follow-up observation of cervical carcinoma, and the stage middle-advanced cervical carcinoma incidence is significantly descended.
The smear that traditional cytolgical examination method conventional smear (being cervical smear) is made, cell is overlapping in a large number, and smear exists a large amount of red blood cells, leucocyte, mucus and the slough etc. that comes off, pathologist is very difficult when interpretation, the false negative rate of 15%-40% can occur.
The liquid based cytology inspection is a cytology new technology of invention at the end of the nineties in last century, and is higher to cervical carcinoma and diagnosing precancerous disease accuracy, is considered to the best approach of cervical carcinoma early diagnosis, early prevention.At present the cell-preservation liquid (PreserveCyt Solution) produced of the new Bai Shi of the U.S. (Thinprer TCT) is and the supporting special agent of its machine Thinprepre-2000, and it is expensive, and a people checks approximately 180 yuan of expenses.
Summary of the invention
The purpose of this invention is to provide a kind of liquid based cell preservative fluid, support the use for import or domestic diaphragm type liquid foot cell pellet mill film-making.
Technical scheme of the present invention: a kind of liquid based cell preservative fluid, it is comprised of alcohols, distilled water, phosphate, bactericidal agent phenol, mucus distintegrant Bromhexine Hydrochloride,
Alcohols: volume percentage 10%-30%, distilled water: the volume percentage supplies 100%;
By the mass body volume concentrations: phosphate-containing 0.1%-3.0% in the liquid based cell preservative fluid, contain phenol 0.1%-2.0%, contain Bromhexine Hydrochloride 0.1%-2.0%;
Described alcohols is selected a kind of in methyl alcohol, ethanol, propyl alcohol, the isopropyl alcohol or any two kinds; The mass concentration of alcohols is 95%-100%;
Described phosphate is selected any one in sodium phosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium phosphate, dipotassium hydrogen phosphate, the potassium dihydrogen phosphate.
The preferred mass volumetric concentration of described mucus distintegrant Bromhexine Hydrochloride in liquid based cell preservative fluid is 1.0%.
Know-why of the present invention:
Because the cast-off cells sample that gathers picks up from the woman cervix uteri position, usually contains a large amount of cervical mucus.The cervical mucus material is a kind of glycoprotein, and the connection between the molecule contains disulfide bond and claims again the S-S key.Bromhexine Hydrochloride, the common name bromhexine hydrochloride has the sticking phlegm effect of strong dissolving, can make the polysaccharide fiber element cracking in the phlegm, and the desaturation sputum is used for reducing phlegm eliminating the phlegm clinically.The present invention is used for the mucus distintegrant to Bromhexine Hydrochloride, and the cell that is wrapped up by mucus in the sample is successfully separated, and has avoided positive cell to pile up and lose, and guarantees effective cell quantity.
Beneficial effect of the present invention:
1. greatly reduce cost.Approximately 120 yuan/cover of the supporting consumptive material price of import, consumables cost of the present invention is about 10 yuan.2. add unique mucus distintegrant Bromhexine Hydrochloride, can successfully separate the cell that is wrapped up by mucus in the sample, avoided positive cell to pile up and lose, thereby improved effective cell quantity.3. add bactericidal agent phenol, can effectively kill the pathogene such as mould, bacterium, virus, Protection environment and staff are healthy.4. suitable phosphate buffering liquid concentration can guarantee the cell attitude that holds its shape, and fixing under the effect of alcohols, made sample is unchanged around preserving.Thereby provide a kind of with low cost, the liquid based cell preservative fluid that result of use is good.Use the cell-preservation liquid of the present invention's development, carry out that the smear background that automatic pelleter film-making makes is Clear ﹠ Transparent, cell monolayer evenly distributes, and is convenient to the sheet of reading of Pathologis.
Description of drawings
The cell-preservation liquid that Fig. 1 uses the present invention to develop carries out the smear that automatic pelleter film-making makes.
Embodiment
The compound method of embodiment 1, liquid based cell preservative fluid of the present invention is: according to the form below composition weighing and measuring
Methyl alcohol 100mL
Sodium phosphate 1.0g
Phenol 1.0g
Bromhexine Hydrochloride 10.0g
Distilled water 900mL
Stirring and dissolving gets final product.
The compound method of embodiment 2, liquid based cell preservative fluid of the present invention is: according to the form below composition weighing and measuring
Methyl alcohol 100mL
Sodium dihydrogen phosphate 5.0g
Phenol 3.0g
Bromhexine Hydrochloride 3.0g
Distilled water 900mL
Stirring and dissolving gets final product.
The compound method of embodiment 3, liquid based cell preservative fluid of the present invention is: according to the form below composition weighing and measuring
Ethanol 100mL
Sodium hydrogen phosphate 5.0g
Phenol 2.0g
Bromhexine Hydrochloride 5.0g
Distilled water 900mL
Stirring and dissolving gets final product.
The compound method of embodiment 4, liquid based cell preservative fluid of the present invention is: according to the form below composition weighing and measuring
Methyl alcohol 100mL
Ethanol 100mL
Potassium phosphate 1.0g
Phenol 2.0g
Bromhexine Hydrochloride 10.0g
Distilled water 800mL
Stirring and dissolving gets final product.
The compound method of embodiment 5, liquid based cell preservative fluid of the present invention is: according to the form below composition weighing and measuring
Isopropyl alcohol 200mL
Potassium dihydrogen phosphate 3.0g
Phenol 2.0g
Bromhexine Hydrochloride 10.0g
Distilled water 800mL
Stirring and dissolving gets final product.
The compound method of embodiment 6, liquid based cell preservative fluid of the present invention is: according to the form below composition weighing and measuring
Ethanol 300mL
Dipotassium hydrogen phosphate 30.0g
Phenol 3.0g
Bromhexine Hydrochloride 8.0g
Distilled water 700mL
Stirring and dissolving gets final product.
Claims (2)
1. a liquid based cell preservative fluid is characterized in that it is comprised of alcohols, distilled water, phosphate, bactericidal agent phenol, mucus distintegrant Bromhexine Hydrochloride,
Alcohols: volume percentage 10%-30%, distilled water: the volume percentage supplies 100%;
By the mass body volume concentrations: phosphate-containing 0.1%-3.0% in the liquid based cell preservative fluid, contain phenol 0.1%-2.0%, contain Bromhexine Hydrochloride 0.1%-2.0%;
Described alcohols is selected a kind of in methyl alcohol, ethanol, propyl alcohol, the isopropyl alcohol or any two kinds; The mass concentration of alcohols is 95%-100%;
Described phosphate is selected any one in sodium phosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium phosphate, dipotassium hydrogen phosphate, the potassium dihydrogen phosphate.
2. liquid based cell preservative fluid according to claim 1, it is characterized in that: the preferred mass volumetric concentration of described mucus distintegrant Bromhexine Hydrochloride in liquid based cell preservative fluid is 1.0%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200910034575 CN101664028B (en) | 2009-09-02 | 2009-09-02 | Liquid based cell preservative fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910034575 CN101664028B (en) | 2009-09-02 | 2009-09-02 | Liquid based cell preservative fluid |
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| CN101664028A CN101664028A (en) | 2010-03-10 |
| CN101664028B true CN101664028B (en) | 2013-04-03 |
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| CN 200910034575 Expired - Fee Related CN101664028B (en) | 2009-09-02 | 2009-09-02 | Liquid based cell preservative fluid |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999343B (en) * | 2010-10-26 | 2013-11-06 | 深圳华大基因健康科技有限公司 | Cell preserving fluid and preparation method and use thereof |
| CN102260731B (en) * | 2011-06-28 | 2015-03-25 | 合肥安旸生物医药有限公司 | Enzyme-labeling-liquid-based cytology staining kit for screening bladder cancer |
| CN102318597B (en) * | 2011-08-19 | 2013-04-03 | 张雪云 | Liquid-based cell preservation liquid composite and preparation method thereof |
| CN103875653B (en) * | 2014-02-11 | 2015-03-18 | 中国人民解放军北京军区总医院 | Endocervical cell preserving fluid and method for preparing endocervical cell specimen |
| CN104642300B (en) * | 2015-03-13 | 2017-01-25 | 上海御康医院有限公司 | Exfoliative cell sap base preserving fluid, method thereof for flaking and kit |
| CN109612800B (en) * | 2019-01-09 | 2021-12-21 | 济南金域医学检验中心有限公司 | Method for preparing non-gynecological liquid-based specimen |
| CN112029824A (en) * | 2020-09-15 | 2020-12-04 | 北京康美天鸿生物科技有限公司 | Nucleic acid preservation solution universally used for multiple samples |
-
2009
- 2009-09-02 CN CN 200910034575 patent/CN101664028B/en not_active Expired - Fee Related
Non-Patent Citations (3)
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| 丁宗博等.保健食品中水溶性多糖的测定.《预防医学文献信息》.1999,(第04期),353. * |
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| CN101664028A (en) | 2010-03-10 |
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