CN106350572A - Vaginitis joint detection kit - Google Patents
Vaginitis joint detection kit Download PDFInfo
- Publication number
- CN106350572A CN106350572A CN201610887124.5A CN201610887124A CN106350572A CN 106350572 A CN106350572 A CN 106350572A CN 201610887124 A CN201610887124 A CN 201610887124A CN 106350572 A CN106350572 A CN 106350572A
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- Prior art keywords
- swab
- reaction
- vaginitis
- detection kit
- detection plate
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- Pending
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- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 206010046914 Vaginal infection Diseases 0.000 title claims abstract description 23
- 201000008100 Vaginitis Diseases 0.000 title claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 11
- 239000003085 diluting agent Substances 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 8
- 102000003992 Peroxidases Human genes 0.000 claims description 5
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 5
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 4
- 108010000849 leukocyte esterase Proteins 0.000 claims description 4
- 229940005654 nitrite ion Drugs 0.000 claims description 4
- 101000588395 Bacillus subtilis (strain 168) Beta-hexosaminidase Proteins 0.000 claims description 3
- 102000005348 Neuraminidase Human genes 0.000 claims description 3
- 108010006232 Neuraminidase Proteins 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 239000000243 solution Substances 0.000 abstract description 7
- 238000005070 sampling Methods 0.000 abstract description 3
- 210000003756 cervix mucus Anatomy 0.000 abstract description 2
- 239000012895 dilution Substances 0.000 abstract description 2
- 238000010790 dilution Methods 0.000 abstract description 2
- 238000012549 training Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
- 210000001215 vagina Anatomy 0.000 description 8
- 230000008859 change Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 208000004926 Bacterial Vaginosis Diseases 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 208000037009 Vaginitis bacterial Diseases 0.000 description 2
- 206010047799 Vulvovaginitis trichomonal Diseases 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000037125 natural defense Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 206010003693 Atrophic vulvovaginitis Diseases 0.000 description 1
- 241000692822 Bacteroidales Species 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 201000007096 Vulvovaginal Candidiasis Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000006713 insertion reaction Methods 0.000 description 1
- 230000003780 keratinization Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- -1 n- acetylglucosamine glycosides Chemical class 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 201000010808 postmenopausal atrophic vaginitis Diseases 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physiology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a vaginitis joint detection kit. The vaginitis joint detection kit comprises a vaginal secretion sterile swab (1), a reaction detection plate (2), a dilution solution dropping bottle (3), a developing solution dropping bottle (4) and a reaction developing colorimetric card which are independently arranged, wherein a sampling hole (21) and a group of reaction holes (22) are formed in the reaction detection plate (2), and the sampling holes (21) are respectively connected with the reaction holes (22) through a drainage trough (23). The detection plate can detect various types of vaginitis, is low in cost, high in detection accuracy, convenient and fast to use and free from professional training for operating staff, and can be used by patients, thus being easy to popularize.
Description
Technical field
The invention belongs to external diagnosis reagent detection field, it is related to a kind of Combined detection kit for vaginitis.
Background technology
Normal healthy women vagina has natural defense function due to the feature of anatomical tissue to the intrusion of pathogen, such as vagina
Mouthful closure, vagina front and rear wall is close to, hypertrophy under the influence of estrogen for the vaginal epithelial cell and cells of superficial layer keratinization, vagina
Acid-base value keeps balance, so that the breeding of the pathogen of adaptation alkalescence is suppressed；However, neck tube mucus is in alkalescence, when vagina
When natural defense function is destroyed, pathogen be easy to invade, lead to colpitis, clinically common have bacterial vaginosis,
Monilial vaginitises, trichomonal vaginitis, senile vaginitiss, infantile vaginitis etc..
Currently used for women vaginitis detection mainly pass through two kinds by way of.First, being smear detection under secretion object lens, this
Plant detection method program complicated, waiting time is long；Second, being women self-detection method, such as Chinese patent
200420111847.9 disclosing a kind of " rapid diagnosis reagent case for bacteroidal colpitis ".But both approaches use all than
More complicated, layman's operation easier is larger, especially the collection of sample is required higher.For patient, not only can increase
Plus sense of discomfort during collecting sample, also can increase feeling of shame and the tension of oneself for young patient, if sample collection is not
When testing result also can be affected.Although having several vaginitiss joint-detection products, processing technology and operation in the market
Method is all more complicated, is not easy to carry out self detection personal.
Content of the invention
Technical problem: in order to solve the defect of prior art, the invention provides a kind of Combined detection kit for vaginitis.
Technical scheme: a kind of Combined detection kit for vaginitis that the present invention provides, including the vaginal secretion being independently arranged
Thing sterile swab, reaction detection plate, diluent drop bottle, nitrite ion drop bottle, reaction solution colorimetric card；Set on described reaction detection plate
There are a well and one group of reacting hole, described well is connected with reacting hole respectively by drainage trough.
As improvement, described vaginal secretionies sterile swab includes the swab body of hollow, swab head, pressurizing unit；Institute
State swab head and be movably connected on swab body one end；Described pressurizing unit includes press section and the push rod interconnecting, press section
Located at the swab body other end, this is internal located at swab for push rod；Pressurizing unit promotes pushrod movement by press section, and push rod promotes
Swab head is separated with swab body；By pressing press section, swab head is extruded from swab body interior, it is to avoid sampling process is subject to
To pollution.
Improve as another kind of, the quantity of described reacting hole is five；Five reaction in the holes fix peroxidase respectively
The solidfied material of reagent, the solidfied material of leukocyte esterase substrate reagent, the solidfied material of neuraminidase substrate reagent, n- acetylamino
The solidfied material of glucosidase substrate reagent and ph reagent paper；Detect 5 indexs, it is to avoid sample-adding causes different index colour developings simultaneously
Time difference.
Improve as another kind of, the well being dimensioned slightly smaller than reaction detection plate of the outlet of described diluent drop bottle
Size；Avoid being loaded the pollution of process sample and sample-adding is uneven.
Improve as another kind of, described drainage trough is from well to the downward-sloping setting of reacting hole, and every drainage trough
Inclination angle is identical.
Beneficial effect: the detection plate that the present invention provides can detect to multiple vaginitiss simultaneously, low cost, detection is accurately
Degree is high, convenient to use, to operator need not specialty training, can be by patient oneself using it is easy to promote.
This Combined detection kit for vaginitis can be used for lactobacilluss, vagina cleanness degree, bacterial vaginosis, mycotic the moon
Road is scorching, the joint inspection of trichomonal vaginitis.
Brief description
Fig. 1 is the structural representation of vaginal secretionies sterile swab；
Fig. 2 is the structural representation of reaction detection plate；
Fig. 3 is the front view of reaction detection plate；
Fig. 4 is the upward view of reaction detection plate；
Fig. 5 is the sectional view of reaction detection plate；
Fig. 6 is the structural representation of diluent drop bottle；
Fig. 7 is the structural representation of nitrite ion drop bottle；
Fig. 8 is the structural representation of reaction solution colorimetric card.
Specific embodiment
Below Combined detection kit for vaginitis of the present invention is further illustrated.
Combined detection kit for vaginitis, including the vaginal secretionies sterile swab 1 (see Fig. 1) being independently arranged, reaction inspection
Drafting board 2 (see Fig. 2-5), diluent drop bottle 3 (see Fig. 6), nitrite ion drop bottle 4 (see Fig. 7), reaction solution colorimetric card 5 (see Fig. 8).
Vaginal secretionies sterile swab 1 includes the swab body 11 of hollow, swab head 12, pressurizing unit 13；Swab head 12
It is movably connected on swab body 11 one end；Pressurizing unit 13 includes press section 14 and the push rod 15 interconnecting, and press section 14 sets
In swab body 11 other end, push rod 15 is in swab body 11；Pressurizing unit 13 promotes push rod 15 to transport by press section 14
Dynamic, push rod 15 promotes swab head 12 to separate with swab body 11.
Reaction detection plate 2 is provided with a well 21 and one group of reacting hole 22, and well 21 passes through drainage trough 23 respectively
It is connected with reacting hole 22.The quantity of reacting hole 22 is five；Consolidating of peroxidase reagent is fixed respectively in five reacting holes 22
Compound, the solidfied material of leukocyte esterase substrate reagent, the solidfied material of neuraminidase substrate reagent, n- acetylglucosamine glycosides
The solidfied material of zymolyte reagent and ph reagent paper；Detect 5 indexs, it is to avoid sample-adding causes the time difference of different index colour developings simultaneously.
Drainage trough 23 is from well 21 to the downward-sloping setting of reacting hole 22, and the inclination angle of every drainage trough 23 is identical.
The size of the well 21 being dimensioned slightly smaller than reaction detection plate 2 of the outlet of diluent drop bottle 3；Avoid being loaded
The pollution of journey sample and sample-adding are uneven.
The using method of this test kit is:
(1) secretions of position after posterior fornix or vagina are taken with vaginal secretionies sterile swab 1；
(2) swab head 12 is squeezed in diluent drop bottle 3 dilution, after the slight concussion several seconds, diluent drop bottle 3 gone out sample
In the well 21 of mouth insertion reaction detection plate 2, reaction detection plate 2 is disposed vertically, extruding diluent drop bottle 3 makes diluted sample
Liquid respectively enters in reacting hole 22 along drainage trough 23；
(3) contrast reaction solution colorimetric card carries out colorimetric, determines whether the positive, can determine whether by color change
With vaginitiss and correlation circumstance；Reaction solution colorimetric card 5 is matched with reaction detection plate, by test strip reaction result with
Color on colorimetric card compares and obtains testing result.Wherein, detection lactobacilluss are to produce color by peroxidase method
Change；Detection vagina cleanness degree is to produce color change by leukocyte esterase method；Detection bacterium vaginosis are by saliva
Acid-enzyme hydrolysis method produces color change, or produces color change by BV-blue test and peroxidase method；Detection mycotic vagina
Inflammation can produce color change by n- acetyl glucosaminidase method；Detection infusorian is by n- acetyl glucosaminidase
Color change and ph value comprehensive descision that method produces.
Claims (5)
1. a kind of Combined detection kit for vaginitis it is characterised in that: include the vaginal secretionies sterile swab that is independently arranged
(1), reaction detection plate (2), diluent drop bottle (3), nitrite ion drop bottle (4), reaction solution colorimetric card (5)；Described reaction detection
Plate (2) is provided with a well (21) and one group of reacting hole (22), described well (21) pass through drainage trough (23) respectively with
Reacting hole (22) connects.
2. a kind of Combined detection kit for vaginitis according to claim 1 it is characterised in that: described vaginal secretionies are no
The swab body (11) of bacterium swab (1) inclusion hollow, swab head (12), pressurizing unit (13)；Described swab head (12) activity is even
It is connected to swab body (11) one end；Described pressurizing unit (13) includes press section (14) and the push rod (15) interconnecting, pressing
Located at swab body (11) other end, push rod (15) is in swab body (11) in portion (14)；Pressurizing unit (13) passes through pressing
Portion (14) promotes push rod (15) motion, and push rod (15) promotes swab head (12) to separate with swab body (11).
3. a kind of Combined detection kit for vaginitis according to claim 1 it is characterised in that: described reacting hole (22)
Quantity is five；Solidfied material, the leukocyte esterase substrate reagent of peroxidase reagent is fixed respectively in five reacting holes (22)
Solidfied material, the solidfied material of neuraminidase substrate reagent, the solidfied material of n- acetyl glucosaminidase substrate reagent and ph
Reagent paper.
4. a kind of Combined detection kit for vaginitis according to claim 1 it is characterised in that: described diluent drop bottle
(3) size of the well (21) being dimensioned slightly smaller than reaction detection plate (2) of outlet.
5. a kind of Combined detection kit for vaginitis according to claim 1 it is characterised in that: described drainage trough (23) from
Well (21) is to the downward-sloping setting of reacting hole (22), and the inclination angle of every drainage trough (23) is identical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887124.5A CN106350572A (en) | 2016-10-10 | 2016-10-10 | Vaginitis joint detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887124.5A CN106350572A (en) | 2016-10-10 | 2016-10-10 | Vaginitis joint detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106350572A true CN106350572A (en) | 2017-01-25 |
Family
ID=57865722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610887124.5A Pending CN106350572A (en) | 2016-10-10 | 2016-10-10 | Vaginitis joint detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106350572A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107099455A (en) * | 2017-05-18 | 2017-08-29 | 广州然益生物科技有限公司 | One kind visualization microbial biochemical identification kit and its authentication method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201514383U (en) * | 2009-09-24 | 2010-06-23 | 姜学英 | Fast detection device for vaginitises |
| CN102253206A (en) * | 2011-06-23 | 2011-11-23 | 泰普生物科学（中国）有限公司 | Combined detection kit for vaginitis |
| CN205215271U (en) * | 2015-12-01 | 2016-05-11 | 董占龙 | Medical sampling swab |
-
2016
- 2016-10-10 CN CN201610887124.5A patent/CN106350572A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201514383U (en) * | 2009-09-24 | 2010-06-23 | 姜学英 | Fast detection device for vaginitises |
| CN102253206A (en) * | 2011-06-23 | 2011-11-23 | 泰普生物科学（中国）有限公司 | Combined detection kit for vaginitis |
| CN205215271U (en) * | 2015-12-01 | 2016-05-11 | 董占龙 | Medical sampling swab |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107099455A (en) * | 2017-05-18 | 2017-08-29 | 广州然益生物科技有限公司 | One kind visualization microbial biochemical identification kit and its authentication method |
| CN107099455B (en) * | 2017-05-18 | 2019-11-29 | 广东环凯微生物科技有限公司 | A kind of visualization microbial biochemical identification kit and its identification method |
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Application publication date: 20170125 |